Prupe.1G314800_v2.0.a1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001447
Physical Location & Seq
Forward (+)
30423350 .. 30424435
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G314800.1

Sequence Viewer

Length: 900 bp
ATGAACGATACAGAGTTGCTATGGAGAGCTTCCATGACTCCTAGGATTTCTGAATATCCATTCCATCGGGTTCCCAAGGTTACTTTTATGTTCATGACAAGGGGACCTGTTGTTTTGGCCCCGTTATGGGAGAAGTTCTTCAAAGGGCATAGAGGCTTGTACTCAATCTATGTGCACTCAAAACCATCCTACAATGCATCATCACACCCTGAAAGTCCAGTTTTTCATGGCCGGAGAATTCCTAGTCAGGAAGTAGAATGGGGAAGGGTGAGCCAGATTGAGGCTGAGCGCCGCCTGTTGGCGAATGCTCTTCTTGACATCGCAAACCAACGTGTTGTTCTGCTCTCAGAATCATGCATCCCCCTCTACAACTTCTACACTGTCTACTCTTACCTCATACACTCCAACGAAACTTTCGTGGAGGTTTATGATGATCCAAGCGTAGACGGACGTGACCGATATTATTTCATTGACTACCCAGGAATCACATTGGATCAATGGAGCAAAGGGTCACAGTGGTTTGAAATTGATAGAGACCTTGCCATTGAAGTTGTCTCCGATCGAATATACTTCCCACTTTTCCTCAGATGCAAAGGCGAATGCTTTGCAGAAGAACATTATTTACCAACATTTGTGCACATGAAATTTGCAGCAAACAGTGCCTACAGGTCTTTGACCTGGGCTGACTGGACCAAGGGTGGCGCGCACCCTACCGAGTACCCGAGCACAAATGTCACATTTGAGCTTTTGAATAGCCTGAGAAATGGTTATGGTCGGAGATGTGAATATAATGGAAGGAGCTCAGATGTCTGTTTCCTATTCGCCAGGAAGTTCCCGCCTACAACATTGGATGGCCTTCTGAGAATTGCACCAAATATCATGCACTTCAGCAACGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

300

Amino Acids

34.82

Weight (kDa)

6.8

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 384, 444
AccII CGCG 1 cut(s) 704
AciI CCGC 2 cut(s) 292, 836
AclWI GGATC 2 cut(s) 428, 501
AcoI YGGCCR 1 cut(s) 229
AcsI RAATTY 2 cut(s) 237, 644
AcuI CTGAAG 1 cut(s) 871
AfaI GTAC 2 cut(s) 161, 719
AfiI CCNNNNNNNGG 4 cut(s) 126, 127, 280, 298
AflIII ACRYGT 1 cut(s) 331
AgsI TTSAA 4 cut(s) 142, 524, 548, 751
AjiI CACGTC 1 cut(s) 452
AjnI CCWGG 3 cut(s) 478, 677, 824
AjuI GAANNNNNNNTTGG 4 cut(s) 321, 353, 398, 430
AluBI AGCT 3 cut(s) 29, 745, 801
AluI AGCT 3 cut(s) 29, 745, 801
Alw21I GWGCWC 4 cut(s) 177, 639, 728, 803
Alw26I GTCTC 2 cut(s) 528, 559
Alw44I GTGCAC 2 cut(s) 173, 635
AlwI GGATC 2 cut(s) 428, 501
Ama87I CYCGRG 1 cut(s) 721
AoxI GGCC 3 cut(s) 117, 229, 853
ApaLI GTGCAC 2 cut(s) 173, 635
ApeKI GCWGC 1 cut(s) 650
ApoI RAATTY 2 cut(s) 237, 644
Asp700I GAANNNNTTC 1 cut(s) 137
AspA2I CCTAGG 1 cut(s) 41
AspLEI GCGC 3 cut(s) 291, 704, 706
AspS9I GGNCC 3 cut(s) 104, 118, 690
AsuHPI GGTGA 1 cut(s) 280
AvaI CYCGRG 1 cut(s) 721
AvaII GGWCC 2 cut(s) 104, 690
AvrII CCTAGG 1 cut(s) 41
BaeGI GKGCMC 2 cut(s) 177, 639
BanII GRGCYC 1 cut(s) 803
Bbv12I GWGCWC 4 cut(s) 177, 639, 728, 803
BbvI GCAGC 1 cut(s) 662
BccI CCATC 3 cut(s) 72, 193, 845
BcgI CGANNNNNNTGC 2 cut(s) 587, 621
BciT130I CCWGG 3 cut(s) 480, 679, 826
BcoDI GTCTC 2 cut(s) 528, 559
BfaI CTAG 2 cut(s) 42, 243
BfmI CTRYAG 1 cut(s) 664
BfoI RGCGCY 1 cut(s) 292
BisI GCNGC 2 cut(s) 292, 651
BlnI CCTAGG 1 cut(s) 41
BlpI GCTNAGC 1 cut(s) 285
BlsI GCNGC 2 cut(s) 293, 652
Bme1390I CCNGG 3 cut(s) 480, 679, 826
Bme18I GGWCC 2 cut(s) 104, 690
BmeT110I CYCGRG 1 cut(s) 721
BmgBI CACGTC 1 cut(s) 452
BmgT120I GGNCC 3 cut(s) 104, 118, 690
BmiI GGNNCC 3 cut(s) 72, 105, 120
BmrFI CCNGG 3 cut(s) 480, 679, 826
BmsI GCATC 3 cut(s) 206, 366, 578
Bpu1102I GCTNAGC 1 cut(s) 285
BsaI GGTCTC 1 cut(s) 528
BsaJI CCNNGG 5 cut(s) 41, 75, 478, 678, 693
Bsc4I CCNNNNNNNGG 4 cut(s) 126, 127, 280, 298
Bse1I ACTGG 2 cut(s) 218, 692
BseBI CCWGG 3 cut(s) 480, 679, 826
BseDI CCNNGG 5 cut(s) 41, 75, 478, 678, 693
BseGI GGATG 3 cut(s) 185, 357, 856
BseLI CCNNNNNNNGG 4 cut(s) 126, 127, 280, 298
BseMII CTCAG 6 cut(s) 276, 360, 598, 749, 816, 851
BseNI ACTGG 2 cut(s) 218, 692
BsePI GCGCGC 1 cut(s) 702
BseSI GKGCMC 2 cut(s) 177, 639
BseXI GCAGC 1 cut(s) 662
Bsh1236I CGCG 1 cut(s) 704
Bsh1285I CGRYCG 1 cut(s) 562
BshFI GGCC 3 cut(s) 119, 231, 855
BsiEI CGRYCG 1 cut(s) 562
BsiHKAI GWGCWC 4 cut(s) 177, 639, 728, 803
BsiHKCI CYCGRG 1 cut(s) 721
BsiSI CCGG 1 cut(s) 232
BslFI GGGAC 1 cut(s) 117
BslI CCNNNNNNNGG 4 cut(s) 126, 127, 280, 298
BsmAI GTCTC 2 cut(s) 528, 559
BsmFI GGGAC 1 cut(s) 117
BsmI GAATGC 2 cut(s) 310, 605
BsnI GGCC 3 cut(s) 119, 231, 855
Bso31I GGTCTC 1 cut(s) 528
BsoBI CYCGRG 1 cut(s) 721
Bsp1286I GDGCHC 4 cut(s) 177, 639, 728, 803
Bsp143I GATC 3 cut(s) 433, 493, 559
Bsp1720I GCTNAGC 1 cut(s) 285
BspACI CCGC 2 cut(s) 292, 836
BspANI GGCC 3 cut(s) 119, 231, 855
BspCNI CTCAG 6 cut(s) 277, 359, 597, 750, 815, 852
BspFNI CGCG 1 cut(s) 704
BspHI TCATGA 1 cut(s) 93
BspLI GGNNCC 3 cut(s) 72, 105, 120
BspPI GGATC 2 cut(s) 428, 501
BspQI GCTCTTC 1 cut(s) 315
BspTNI GGTCTC 1 cut(s) 528
BsrI ACTGG 2 cut(s) 218, 692
BssECI CCNNGG 5 cut(s) 41, 75, 478, 678, 693
BssHII GCGCGC 1 cut(s) 702
BssMI GATC 3 cut(s) 433, 493, 559
BssT1I CCWWGG 3 cut(s) 41, 75, 693
Bst2UI CCWGG 3 cut(s) 480, 679, 826
Bst4CI ACNGT 3 cut(s) 382, 516, 659
Bst6I CTCTTC 1 cut(s) 315
BstAPI GCANNNNNTGC 1 cut(s) 659
BstC8I GCNNGC 1 cut(s) 704
BstDEI CTNAG 6 cut(s) 285, 346, 584, 758, 802, 860
BstF5I GGATG 3 cut(s) 185, 357, 856
BstFNI CGCG 1 cut(s) 704
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 3 cut(s) 291, 704, 706
BstKTI GATC 3 cut(s) 436, 496, 562
BstMAI GTCTC 2 cut(s) 528, 559
BstMBI GATC 3 cut(s) 433, 493, 559
BstMCI CGRYCG 1 cut(s) 562
BstMWI GCNNNNNNNGC 1 cut(s) 659
BstNI CCWGG 3 cut(s) 480, 679, 826
BstSCI CCNGG 3 cut(s) 478, 677, 824
BstSFI CTRYAG 1 cut(s) 664
BstSLI GKGCMC 2 cut(s) 177, 639
BstUI CGCG 1 cut(s) 704
BstV1I GCAGC 1 cut(s) 662
BsuRI GGCC 3 cut(s) 119, 231, 855
BtgZI GCGATG 1 cut(s) 304
BtrI CACGTC 1 cut(s) 452
BtsCI GGATG 3 cut(s) 185, 357, 856
BtsIMutI CAGTG 3 cut(s) 378, 521, 664
Cac8I GCNNGC 1 cut(s) 704
CciI TCATGA 1 cut(s) 93
CfoI GCGC 3 cut(s) 291, 704, 706
Cfr13I GGNCC 3 cut(s) 104, 118, 690
Csp6I GTAC 2 cut(s) 160, 718
CviAII CATG 6 cut(s) 34, 94, 227, 354, 640, 880
CviQI GTAC 2 cut(s) 160, 718
DdeI CTNAG 6 cut(s) 285, 346, 584, 758, 802, 860
DpnI GATC 3 cut(s) 435, 495, 561
DpnII GATC 3 cut(s) 433, 493, 559
EaeI YGGCCR 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Ecl136II GAGCTC 1 cut(s) 801
Eco130I CCWWGG 3 cut(s) 41, 75, 693
Eco24I GRGCYC 1 cut(s) 803
Eco31I GGTCTC 1 cut(s) 528
Eco47I GGWCC 2 cut(s) 104, 690
Eco53kI GAGCTC 1 cut(s) 801
Eco57I CTGAAG 1 cut(s) 871
Eco88I CYCGRG 1 cut(s) 721
EcoICRI GAGCTC 1 cut(s) 801
EcoO109I RGGNCCY 1 cut(s) 104
EcoRI GAATTC 1 cut(s) 237
EcoRII CCWGG 3 cut(s) 478, 677, 824
EcoT14I CCWWGG 3 cut(s) 41, 75, 693
EcoT22I ATGCAT 2 cut(s) 199, 359
EcoT38I GRGCYC 1 cut(s) 803
ErhI CCWWGG 3 cut(s) 41, 75, 693
FaeI CATG 6 cut(s) 37, 97, 230, 357, 643, 883
FaqI GGGAC 1 cut(s) 117
FatI CATG 6 cut(s) 33, 93, 226, 353, 639, 879
FauI CCCGC 1 cut(s) 843
FblI GTMKAC 2 cut(s) 384, 444
Fnu4HI GCNGC 2 cut(s) 292, 651
FokI GGATG 3 cut(s) 172, 344, 863
FriOI GRGCYC 1 cut(s) 803
Fsp4HI GCNGC 2 cut(s) 292, 651
FspBI CTAG 2 cut(s) 42, 243
GlaI GCGC 3 cut(s) 290, 703, 705
GluI GCNGC 2 cut(s) 292, 651
HaeII RGCGCY 1 cut(s) 292
HaeIII GGCC 3 cut(s) 119, 231, 855
HapII CCGG 1 cut(s) 232
HhaI GCGC 3 cut(s) 291, 704, 706
Hin1II CATG 6 cut(s) 37, 97, 230, 357, 643, 883
Hin6I GCGC 3 cut(s) 289, 702, 704
HinP1I GCGC 3 cut(s) 289, 702, 704
HinfI GANTC 3 cut(s) 37, 350, 483
HpaII CCGG 1 cut(s) 232
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 4 cut(s) 175, 385, 445, 637
Hpy188I TCNGA 7 cut(s) 52, 349, 559, 587, 777, 805, 861
Hpy188III TCNNGA 3 cut(s) 94, 248, 314
Hpy8I GTNNAC 4 cut(s) 175, 385, 445, 637
HpyAV CCTTC 3 cut(s) 258, 789, 866
HpyCH4III ACNGT 3 cut(s) 382, 516, 659
HpyCH4IV ACGT 2 cut(s) 331, 451
HpyCH4V TGCA 9 cut(s) 175, 197, 357, 591, 608, 637, 650, 869, 883
HpyF10VI GCNNNNNNNGC 1 cut(s) 659
HpyF3I CTNAG 6 cut(s) 285, 346, 584, 758, 802, 860
HpySE526I ACGT 2 cut(s) 331, 451
Hsp92II CATG 6 cut(s) 37, 97, 230, 357, 643, 883
HspAI GCGC 3 cut(s) 289, 702, 704
Kzo9I GATC 3 cut(s) 433, 493, 559
LguI GCTCTTC 1 cut(s) 315
LmnI GCTCC 2 cut(s) 501, 798
Lsp1109I GCAGC 1 cut(s) 662
LweI GCATC 3 cut(s) 206, 366, 578
MaeI CTAG 2 cut(s) 42, 243
MaeII ACGT 2 cut(s) 331, 451
MaeIII GTNAC 4 cut(s) 79, 452, 510, 733
MalI GATC 3 cut(s) 435, 495, 561
MboI GATC 3 cut(s) 433, 493, 559
MboII GAAGA 3 cut(s) 130, 302, 623
MhlI GDGCHC 4 cut(s) 177, 639, 728, 803
MluCI AATT 4 cut(s) 237, 525, 644, 864
MlyI GAGTC 1 cut(s) 31
MmeI TCCRAC 2 cut(s) 429, 755
MnlI CCTC 6 cut(s) 146, 274, 374, 404, 415, 593
Mph1103I ATGCAT 2 cut(s) 199, 359
MroXI GAANNNNTTC 1 cut(s) 137
MspI CCGG 1 cut(s) 232
MspR9I CCNGG 3 cut(s) 480, 679, 826
Mva1269I GAATGC 2 cut(s) 310, 605
MvaI CCWGG 3 cut(s) 480, 679, 826
MvnI CGCG 1 cut(s) 704
MwoI GCNNNNNNNGC 1 cut(s) 659
NdeII GATC 3 cut(s) 433, 493, 559
NlaIII CATG 6 cut(s) 37, 97, 230, 357, 643, 883
NlaIV GGNNCC 3 cut(s) 72, 105, 120
NmuCI GTSAC 3 cut(s) 452, 510, 733
NsiI ATGCAT 2 cut(s) 199, 359
PagI TCATGA 1 cut(s) 93
PauI GCGCGC 1 cut(s) 702
PciSI GCTCTTC 1 cut(s) 315
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PctI GAATGC 2 cut(s) 310, 605
PdmI GAANNNNTTC 1 cut(s) 137
PfeI GAWTC 2 cut(s) 350, 483
PkrI GCNGC 2 cut(s) 293, 652
Ple19I CGATCG 1 cut(s) 562
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
PpuMI RGGWCCY 1 cut(s) 104
Psp124BI GAGCTC 1 cut(s) 803
Psp5II RGGWCCY 1 cut(s) 104
Psp6I CCWGG 3 cut(s) 478, 677, 824
PspGI CCWGG 3 cut(s) 478, 677, 824
PspN4I GGNNCC 3 cut(s) 72, 105, 120
PspPI GGNCC 3 cut(s) 104, 118, 690
PspPPI RGGWCCY 1 cut(s) 104
PsrI GAACNNNNNNTAC 2 cut(s) 606, 638
PteI GCGCGC 1 cut(s) 702
PvuI CGATCG 1 cut(s) 562
RsaI GTAC 2 cut(s) 161, 719
RsaNI GTAC 2 cut(s) 160, 718
SacI GAGCTC 1 cut(s) 803
SapI GCTCTTC 1 cut(s) 315
SatI GCNGC 2 cut(s) 292, 651
Sau3AI GATC 3 cut(s) 433, 493, 559
Sau96I GGNCC 3 cut(s) 104, 118, 690
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 3 cut(s) 480, 679, 826
SduI GDGCHC 4 cut(s) 177, 639, 728, 803
SfaNI GCATC 3 cut(s) 206, 366, 578
SfcI CTRYAG 1 cut(s) 664
SinI GGWCC 2 cut(s) 104, 690
Sse9I AATT 4 cut(s) 237, 525, 644, 864
SsiI CCGC 2 cut(s) 292, 836
SspMI CTAG 2 cut(s) 42, 243
SstI GAGCTC 1 cut(s) 803
StyD4I CCNGG 3 cut(s) 478, 677, 824
StyI CCWWGG 3 cut(s) 41, 75, 693
TaaI ACNGT 3 cut(s) 382, 516, 659
TaiI ACGT 2 cut(s) 334, 454
TaqI TCGA 1 cut(s) 562
TaqII GACCGA 1 cut(s) 471
TasI AATT 4 cut(s) 237, 525, 644, 864
TatI WGTACW 1 cut(s) 159
TauI GCSGC 1 cut(s) 294
TfiI GAWTC 2 cut(s) 350, 483
TscAI CASTG 3 cut(s) 385, 521, 664
TseFI GTSAC 3 cut(s) 452, 510, 733
TseI GCWGC 1 cut(s) 650
Tsp45I GTSAC 3 cut(s) 452, 510, 733
TspDTI ATGAA 5 cut(s) 17, 82, 215, 457, 656
TspGWI ACGGA 1 cut(s) 462
TspRI CASTG 3 cut(s) 385, 521, 664
VneI GTGCAC 2 cut(s) 173, 635
VpaK11BI GGWCC 2 cut(s) 104, 690
XapI RAATTY 2 cut(s) 237, 644
XmaJI CCTAGG 1 cut(s) 41
XmiI GTMKAC 2 cut(s) 384, 444
XmnI GAANNNNTTC 1 cut(s) 137
XspI CTAG 2 cut(s) 42, 243
Zsp2I ATGCAT 2 cut(s) 199, 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.