FvH4_4g26972

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001447
Physical Location & Seq
Reverse (-)
28150172 .. 28152408
2237 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26972.t1

Sequence Viewer

Length: 1239 bp
ATGAAGGCTCAGAATCAAAACCCTTCGAAGTTCTTCACGAAGCAAATCAATGTCCAAGTCCTCTCTTATTTCCTCATCTTTGGTGGTGGTTTAGTCCTTGGAATCGTACTTGGCTCCTTTTATCTGAAGGACTCTTCCTTTAGTCTTCAAATAGCCGAAATGTCATTCTCTTCTTCTCCTTCTTCTTCTGCTTCATCTCCATCCGATAATGCAAATATTTCACATATGCTAGTTAGTCCAAATGACACAGTCACTATGTCTAACATAACCGAAGTTCATATAGGAGCATTAGAGGAATTCCTAAAACCGCCTAAGGTTTGGCACGACATGGATGATGAGGAATTGCTCTGGAGAGCTTCCATGGCCCCTCGGATACTCCAATATCCATTCAAGCGAGTTCCGAAAGTTGCTTTCATGTTCTTGACAAGAGGGCCTATTCATTTGGCACCATTGTGGGAGAAGTTCTTCCAAGGGCATCAAGGCTTATACTCGATCTATATCCACTCAGATATGTATTACAATGAATCCTCTCACCCGGAAAGTCCAATTTTTCGTGGCAGACGAATCCCAAGTCAGAGAGTAGATTGGGGACGAGTGAACTTGATTGAAGCCGAGAGCCGCCTAATAGCGAATGCTCTTCTCGACGTCTCAAACCAACGTTTTGTTCTGCTTTCGGAAGCATGCATCCCCCTCTTCAACTTCTCCACTGTGTACTCCTACCTCATAAACACCCACCAGACTTTTGTTCAAGTCTATGATGATCCAAGCGGAGTTGGGCGTGGCCGATATAATGAAAGCTACTACCCTCAAATCACATTGGACCAGTGGAGGAAAGGGTCGCAGTGGGTTGAAATAGATAGAGACATTGCCATTGAAGTTGTTTCAGATCAAAAATACTACACAACTTTCCAGAAACTATGCAGAGGCTATTGCTTTGCAGATGAGCATTACTTGCCAACATTTATGAACATAAGATTTCCAACAAAGAACTCAAATAGGAGTGTGACCTGGGTTGATTGGTCTACGGGCGGACCTCATCCTGTCGAGTATGAGAGCACAGATGTCACGGTTGAGCTTCTGAATAAGCTGAGGCATGGCTCCTGGTGTGAATACAATGGAAGGGGCACAAATGTTTGTTTCTTGTTCGCGCGAAAATTCCCGCTAAGTTCTTTGGATACCCTGCTGAAGATTGCACCACAGGTAATGCAGTTTAACACAGACATTGATAAGGAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

413

Amino Acids

47.42

Weight (kDa)

6.27

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 136 - 364 9.6e-75 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 648
AccB1I GGYRCC 1 cut(s) 445
AccI GTMKAC 1 cut(s) 1022
AccII CGCG 2 cut(s) 1148, 1150
AciI CCGC 5 cut(s) 308, 619, 768, 1029, 1160
AclI AACGTT 1 cut(s) 658
AclWI GGATC 1 cut(s) 755
AcoI YGGCCR 1 cut(s) 781
AcsI RAATTY 2 cut(s) 296, 1154
AcuI CTGAAG 2 cut(s) 146, 1205
AcyI GRCGYC 1 cut(s) 645
AfaI GTAC 3 cut(s) 108, 713, 1235
AgsI TTSAA 7 cut(s) 149, 391, 608, 697, 749, 851, 875
AjnI CCWGG 2 cut(s) 1007, 1100
AjuI GAANNNNNNNTTGG 2 cut(s) 648, 680
AleI CACNNNNGTG 1 cut(s) 451
AluBI AGCT 4 cut(s) 356, 798, 1075, 1087
AluI AGCT 4 cut(s) 356, 798, 1075, 1087
Alw21I GWGCWC 1 cut(s) 1058
Alw26I GTCTC 2 cut(s) 652, 855
AlwI GGATC 1 cut(s) 755
AoxI GGCC 3 cut(s) 363, 431, 781
ApoI RAATTY 2 cut(s) 296, 1154
ArsI GACNNNNNNTTYG 2 cut(s) 556, 588
Asp700I GAANNNNTTC 2 cut(s) 32, 464
AspLEI GCGC 1 cut(s) 1150
AspS9I GGNCC 4 cut(s) 364, 431, 820, 1031
AsuC2I CCSGG 1 cut(s) 536
AsuHPI GGTGA 1 cut(s) 524
AsuII TTCGAA 1 cut(s) 26
AvaII GGWCC 2 cut(s) 820, 1031
AxyI CCTNAGG 1 cut(s) 312
BaeGI GKGCMC 1 cut(s) 1127
BanI GGYRCC 1 cut(s) 445
BbsI GAAGAC 1 cut(s) 137
Bbv12I GWGCWC 1 cut(s) 1058
BbvCI CCTCAGC 1 cut(s) 1088
BccI CCATC 1 cut(s) 208
BciT130I CCWGG 2 cut(s) 1009, 1102
BciVI GTATCC 2 cut(s) 366, 1168
BcnI CCSGG 1 cut(s) 536
BcoDI GTCTC 2 cut(s) 652, 855
BfaI CTAG 2 cut(s) 230, 1237
BfuI GTATCC 2 cut(s) 366, 1168
BisI GCNGC 1 cut(s) 619
BlsI GCNGC 1 cut(s) 620
BmcAI AGTACT 1 cut(s) 1235
Bme1390I CCNGG 3 cut(s) 536, 1009, 1102
Bme18I GGWCC 2 cut(s) 820, 1031
BmgT120I GGNCC 4 cut(s) 364, 431, 820, 1031
BmiI GGNNCC 4 cut(s) 115, 366, 447, 1099
BmrFI CCNGG 3 cut(s) 536, 1009, 1102
BmsI GCATC 2 cut(s) 484, 693
BpiI GAAGAC 1 cut(s) 137
BpmI CTGGAG 1 cut(s) 370
Bpu10I CCTNAGC 1 cut(s) 1088
Bpu14I TTCGAA 1 cut(s) 26
BpuMI CCSGG 1 cut(s) 536
BsaBI GATNNNNATC 1 cut(s) 497
BsaHI GRCGYC 1 cut(s) 645
BsaJI CCNNGG 5 cut(s) 97, 360, 368, 469, 1008
Bse1I ACTGG 1 cut(s) 823
Bse21I CCTNAGG 1 cut(s) 312
Bse3DI GCAATG 1 cut(s) 864
Bse8I GATNNNNATC 1 cut(s) 497
BseBI CCWGG 2 cut(s) 1009, 1102
BseDI CCNNGG 5 cut(s) 97, 360, 368, 469, 1008
BseGI GGATG 4 cut(s) 200, 337, 684, 1036
BseJI GATNNNNATC 1 cut(s) 497
BseMI GCAATG 1 cut(s) 864
BseMII CTCAG 3 cut(s) 23, 519, 1079
BseNI ACTGG 1 cut(s) 823
BseSI GKGCMC 1 cut(s) 1127
Bsh1236I CGCG 2 cut(s) 1148, 1150
BshFI GGCC 3 cut(s) 365, 433, 783
BshNI GGYRCC 1 cut(s) 445
BsiHKAI GWGCWC 1 cut(s) 1058
BsiSI CCGG 1 cut(s) 536
BslFI GGGAC 1 cut(s) 603
BsmAI GTCTC 2 cut(s) 652, 855
BsmBI CGTCTC 1 cut(s) 652
BsmFI GGGAC 1 cut(s) 603
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 3 cut(s) 365, 433, 783
Bsp119I TTCGAA 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 1058, 1127
Bsp143I GATC 3 cut(s) 492, 760, 886
Bsp19I CCATGG 1 cut(s) 360
BspACI CCGC 5 cut(s) 308, 619, 768, 1029, 1160
BspANI GGCC 3 cut(s) 365, 433, 783
BspCNI CTCAG 3 cut(s) 22, 518, 1080
BspFNI CGCG 2 cut(s) 1148, 1150
BspLI GGNNCC 4 cut(s) 115, 366, 447, 1099
BspPI GGATC 1 cut(s) 755
BspQI GCTCTTC 1 cut(s) 642
BspT104I TTCGAA 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 445
BsrDI GCAATG 1 cut(s) 864
BsrI ACTGG 1 cut(s) 823
BssECI CCNNGG 5 cut(s) 97, 360, 368, 469, 1008
BssMI GATC 3 cut(s) 492, 760, 886
BssNI GRCGYC 1 cut(s) 645
BssT1I CCWWGG 3 cut(s) 97, 360, 469
Bst2UI CCWGG 2 cut(s) 1009, 1102
Bst4CI ACNGT 3 cut(s) 250, 709, 1069
Bst6I CTCTTC 4 cut(s) 139, 175, 642, 698
BstACI GRCGYC 1 cut(s) 645
BstAPI GCANNNNNTGC 1 cut(s) 952
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 682
BstDEI CTNAG 5 cut(s) 9, 312, 505, 1088, 1163
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 4 cut(s) 200, 337, 684, 1036
BstFNI CGCG 2 cut(s) 1148, 1150
BstHHI GCGC 1 cut(s) 1150
BstKTI GATC 3 cut(s) 495, 763, 889
BstMAI GTCTC 2 cut(s) 652, 855
BstMBI GATC 3 cut(s) 492, 760, 886
BstMWI GCNNNNNNNGC 2 cut(s) 362, 952
BstNI CCWGG 2 cut(s) 1009, 1102
BstNSI RCATGY 1 cut(s) 684
BstSCI CCNGG 3 cut(s) 534, 1007, 1100
BstSLI GKGCMC 1 cut(s) 1127
BstUI CGCG 2 cut(s) 1148, 1150
BstV2I GAAGAC 1 cut(s) 137
Bsu36I CCTNAGG 1 cut(s) 312
BsuI GTATCC 2 cut(s) 366, 1168
BsuRI GGCC 3 cut(s) 365, 433, 783
BtgI CCRYGG 1 cut(s) 360
BtsCI GGATG 4 cut(s) 200, 337, 684, 1036
BtsI GCAGTG 1 cut(s) 848
BtsIMutI CAGTG 3 cut(s) 705, 830, 848
Cac8I GCNNGC 1 cut(s) 682
CfoI GCGC 1 cut(s) 1150
Cfr13I GGNCC 4 cut(s) 364, 431, 820, 1031
Csp6I GTAC 3 cut(s) 107, 712, 1234
CviAII CATG 5 cut(s) 328, 361, 415, 681, 1094
CviQI GTAC 3 cut(s) 107, 712, 1234
DdeI CTNAG 5 cut(s) 9, 312, 505, 1088, 1163
DpnI GATC 3 cut(s) 494, 762, 888
DpnII GATC 3 cut(s) 492, 760, 886
EaeI YGGCCR 1 cut(s) 781
Eam1104I CTCTTC 4 cut(s) 139, 175, 642, 698
EarI CTCTTC 4 cut(s) 139, 175, 642, 698
EciI GGCGGA 1 cut(s) 1044
Eco130I CCWWGG 3 cut(s) 97, 360, 469
Eco47I GGWCC 2 cut(s) 820, 1031
Eco57I CTGAAG 2 cut(s) 146, 1205
Eco81I CCTNAGG 1 cut(s) 312
EcoO109I RGGNCCY 1 cut(s) 431
EcoRI GAATTC 1 cut(s) 296
EcoRII CCWGG 2 cut(s) 1007, 1100
EcoT14I CCWWGG 3 cut(s) 97, 360, 469
EcoT22I ATGCAT 1 cut(s) 686
ErhI CCWWGG 3 cut(s) 97, 360, 469
Esp3I CGTCTC 1 cut(s) 652
FaeI CATG 5 cut(s) 331, 364, 418, 684, 1097
FaqI GGGAC 1 cut(s) 603
FatI CATG 5 cut(s) 327, 360, 414, 680, 1093
FauI CCCGC 1 cut(s) 1167
FauNDI CATATG 1 cut(s) 225
FblI GTMKAC 1 cut(s) 1022
Fnu4HI GCNGC 1 cut(s) 619
FokI GGATG 4 cut(s) 187, 344, 671, 1023
Fsp4HI GCNGC 1 cut(s) 619
FspBI CTAG 2 cut(s) 230, 1237
GlaI GCGC 1 cut(s) 1149
GluI GCNGC 1 cut(s) 619
GsuI CTGGAG 1 cut(s) 370
HaeIII GGCC 3 cut(s) 365, 433, 783
HapII CCGG 1 cut(s) 536
HhaI GCGC 1 cut(s) 1150
Hin1I GRCGYC 1 cut(s) 645
Hin1II CATG 5 cut(s) 331, 364, 418, 684, 1097
Hin6I GCGC 1 cut(s) 1148
HinP1I GCGC 1 cut(s) 1148
HinfI GANTC 5 cut(s) 13, 102, 131, 524, 564
HpaII CCGG 1 cut(s) 536
HphI GGTGA 1 cut(s) 524
Hpy166II GTNNAC 3 cut(s) 598, 712, 1023
Hpy188III TCNNGA 5 cut(s) 37, 349, 421, 641, 910
Hpy8I GTNNAC 3 cut(s) 598, 712, 1023
Hpy99I CGWCG 1 cut(s) 647
HpyAV CCTTC 4 cut(s) 33, 121, 189, 1113
HpyCH4III ACNGT 3 cut(s) 250, 709, 1069
HpyCH4IV ACGT 2 cut(s) 645, 658
HpyCH4V TGCA 6 cut(s) 212, 684, 921, 938, 1193, 1207
HpyF10VI GCNNNNNNNGC 2 cut(s) 362, 952
HpyF3I CTNAG 5 cut(s) 9, 312, 505, 1088, 1163
HpySE526I ACGT 2 cut(s) 645, 658
Hsp92I GRCGYC 1 cut(s) 645
Hsp92II CATG 5 cut(s) 331, 364, 418, 684, 1097
HspAI GCGC 1 cut(s) 1148
Kzo9I GATC 3 cut(s) 492, 760, 886
LguI GCTCTTC 1 cut(s) 642
LmnI GCTCC 3 cut(s) 119, 284, 1103
LweI GCATC 2 cut(s) 484, 693
MaeI CTAG 2 cut(s) 230, 1237
MaeII ACGT 2 cut(s) 645, 658
MaeIII GTNAC 3 cut(s) 250, 1003, 1063
MalI GATC 3 cut(s) 494, 762, 888
MboI GATC 3 cut(s) 492, 760, 886
MhlI GDGCHC 2 cut(s) 1058, 1127
MluCI AATT 4 cut(s) 296, 341, 546, 1154
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 1004
Mph1103I ATGCAT 1 cut(s) 686
MroXI GAANNNNTTC 2 cut(s) 32, 464
MseI TTAA 1 cut(s) 1212
MslI CAYNNNNRTG 1 cut(s) 451
MspI CCGG 1 cut(s) 536
MspR9I CCNGG 3 cut(s) 536, 1009, 1102
Mva1269I GAATGC 1 cut(s) 637
MvaI CCWGG 2 cut(s) 1009, 1102
MvnI CGCG 2 cut(s) 1148, 1150
MwoI GCNNNNNNNGC 2 cut(s) 362, 952
NciI CCSGG 1 cut(s) 536
NcoI CCATGG 1 cut(s) 360
NdeI CATATG 1 cut(s) 225
NdeII GATC 3 cut(s) 492, 760, 886
NlaIII CATG 5 cut(s) 331, 364, 418, 684, 1097
NlaIV GGNNCC 4 cut(s) 115, 366, 447, 1099
NmeAIII GCCGAG 1 cut(s) 637
NmuCI GTSAC 3 cut(s) 250, 1003, 1063
NsiI ATGCAT 1 cut(s) 686
NspI RCATGY 1 cut(s) 684
NspV TTCGAA 1 cut(s) 26
OliI CACNNNNGTG 1 cut(s) 451
PaeI GCATGC 1 cut(s) 684
PciSI GCTCTTC 1 cut(s) 642
PcsI WCGNNNNNNNCGW 1 cut(s) 559
PctI GAATGC 1 cut(s) 637
PdmI GAANNNNTTC 2 cut(s) 32, 464
PfeI GAWTC 4 cut(s) 13, 102, 524, 564
PflFI GACNNNGTC 1 cut(s) 248
PkrI GCNGC 1 cut(s) 620
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
Psp1406I AACGTT 1 cut(s) 658
Psp6I CCWGG 2 cut(s) 1007, 1100
PspGI CCWGG 2 cut(s) 1007, 1100
PspN4I GGNNCC 4 cut(s) 115, 366, 447, 1099
PspPI GGNCC 4 cut(s) 364, 431, 820, 1031
PsyI GACNNNGTC 1 cut(s) 248
RsaI GTAC 3 cut(s) 108, 713, 1235
RsaNI GTAC 3 cut(s) 107, 712, 1234
RseI CAYNNNNRTG 1 cut(s) 451
SapI GCTCTTC 1 cut(s) 642
SaqAI TTAA 1 cut(s) 1212
SatI GCNGC 1 cut(s) 619
Sau3AI GATC 3 cut(s) 492, 760, 886
Sau96I GGNCC 4 cut(s) 364, 431, 820, 1031
ScaI AGTACT 1 cut(s) 1235
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 3 cut(s) 536, 1009, 1102
SduI GDGCHC 2 cut(s) 1058, 1127
SfaNI GCATC 2 cut(s) 484, 693
SfuI TTCGAA 1 cut(s) 26
SinI GGWCC 2 cut(s) 820, 1031
SmiMI CAYNNNNRTG 1 cut(s) 451
SphI GCATGC 1 cut(s) 684
Sse9I AATT 4 cut(s) 296, 341, 546, 1154
SsiI CCGC 5 cut(s) 308, 619, 768, 1029, 1160
SspI AATATT 1 cut(s) 217
SspMI CTAG 2 cut(s) 230, 1237
StyD4I CCNGG 3 cut(s) 534, 1007, 1100
StyI CCWWGG 3 cut(s) 97, 360, 469
TaaI ACNGT 3 cut(s) 250, 709, 1069
TaiI ACGT 2 cut(s) 648, 661
TaqI TCGA 4 cut(s) 26, 491, 642, 1044
TasI AATT 4 cut(s) 296, 341, 546, 1154
TatI WGTACW 2 cut(s) 711, 1233
TauI GCSGC 1 cut(s) 621
TfiI GAWTC 4 cut(s) 13, 102, 524, 564
Tru1I TTAA 1 cut(s) 1212
Tru9I TTAA 1 cut(s) 1212
TscAI CASTG 3 cut(s) 712, 830, 848
TseFI GTSAC 3 cut(s) 250, 1003, 1063
Tsp45I GTSAC 3 cut(s) 250, 1003, 1063
TspDTI ATGAA 8 cut(s) 17, 183, 266, 403, 428, 537, 807, 980
TspRI CASTG 3 cut(s) 712, 830, 848
Tth111I GACNNNGTC 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 820, 1031
XapI RAATTY 2 cut(s) 296, 1154
XceI RCATGY 1 cut(s) 684
XmiI GTMKAC 1 cut(s) 1022
XmnI GAANNNNTTC 2 cut(s) 32, 464
XspI CTAG 2 cut(s) 230, 1237
ZraI GACGTC 1 cut(s) 646
ZrmI AGTACT 1 cut(s) 1235
Zsp2I ATGCAT 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.