Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000740

Physical Location & Seq

Genomic Coordinates Forward (+)

344908 .. 348847

Gene Structure 3940 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G02030.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1989 bp

ATGGCGGCCACCGGATTTCACACGCTCTCTGCCGTCGGATTATTCAACGGAGAGAAGCACAAGCACCCCCTTGGAGTAACCCTACCCCCGGCACGCCGATTCTCCGCTTCTGTCACCGTTTTTAGCTCGAGGCTTGTGGACTACGAGCAGCCATTCGTTAAGAGCTTCTGGAACTACCTCGAGGCGGCTAAGGATTTCATCAGACCGGAAGACAACGGCCCATCTCGCTGGTTCTCTCCCCTCGAAAGAAGCAAGGATCCATGTGACGGTGCCCCGCTCCTTCTCTTCCTACCTGGTATCGACGGAAATGGACTCGGGCTAATAAGGCAGCACCAGAAACTTGGACAGATGTTTGATATCTGGTGCTTGCACATACCAGCGAGCAATCGCTCTTCTTTCACAGATCTAGTCGCCATGGTTGAAACAACTGTCAAATATGAAAATCAACGATCACCTGGGAAACCCATATATCTTGTTGGAGAATCTCTCGGCGCCTGCATTGCACTCGCTGTTGCAGCCTGTAACCCTGATATTGATCTTGTCTTGATTCTTTCTAACCCAGCTACATCATTTGGGCATTCTTCGCTGCAACATCTTGCTCCCCTGGTTAAAGCCTTGCCAGACCAACTTAATCTTGCATTTCCTTCCGTGCTAAGCTTGATTCCAGGTGGCCCTTTAAAAAGGATGGTTGCTCACTGGGTCAGAGGACTTCCGGAGACGGAAACAGCTGCAAACATTTTTCAAGATTTGGTGATAACATCCACATTAACCTCTATCCTAGCTGATACATTTCGGAGAGAAACTCTTCTGTGGAAGCTCAAGTTGCTTGATGCCGCTGCTATTTTTGCCAATGCACATCTACATCTAGTACAAGCTCAGACTCTAATTCTGTCCAGCGGAAATGACCAAATACTACCCAGTAAATATGAAGGCAAACGACTTCGGAAAAAACTGCTTAAATGTGAAGTCCGCTCATTCAAAGACAACGGCCATTGCCTTTTTCTGGAGGACGGCATTGATCTGGTCAGTATCATAAAAGCCACTTCTTTTTACCGGCGTGGGTCTCGTCAGGATTACGTCTCCGACTACATCCCACCAACCATCTCTGAGTTTAACAAAAGTTATGGGATCAACAGGCTTCTTGAGGTGATCATGGGCCCTGTATTTCTGTCGACGACAGAAGATGGGAAAGTGGTGAGGGGTCTTGGGGGAATTCCATCAGAAGGACCTGTACTGTTGGTGGGCAATCACATGCTACTGGCGAGTGACAAAATATCACTCCCAGGACAATTTGTTCACGAGAGGAACATCAATTTGCGGCCTCTTGTGCACCCCATGATGTTTTCGAGACTGAGAGATGGATTGTTACCTGATGTGTCTGTTTATGACATGCTCAGGATGATGGGGTCGGTCCCCATCTCGGGCACTCACCTCCATAATCTCTTGTCTGCTAAATCCCATATTCTGTTGTTTCCAGGAGGCATCCGCGAAGCTCTACACCAATACAAGTTGATGTGGCCAGAGAAAGCAGAGTTTGTTAGAGCTGCAGCCAAATTTGGAGCCAAAATAGTCCCCTTTTGTGGGGTTGGGGAAGATGATTTTTTAAAAGTGGTCGTAGACTACAATGACCAGATAAAGGTTCCGTTGGTGAAAGAGGTGCTAAAAAGAGTGACAGCGGAAGGCCCGGAGGTGAGGGGAAGCCTGGAAGGAGAAGAGGGGAACCAAGACTTCCACATGCCAGGAGTGATACCAAAGTGTCCAGGGAGATACTATTATTACTTTGGGAAGGAGATAAAGACGGGAGCAGAAGAGTTGAGAGACAGAGACAAGGCAAAGGAGGTGTACGCAGACGTGAAGAAGGAGGTCGAGAGATGCATAAAGTTTGTGAAGCAGAGAAGGGAGGAGGATCCTTACAGACCTCTCTTGCCCCGTCTCAAATATCACCTGCAGCACGGCCTCCTCTCCCAAGTCCCCACTTTCCACTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010287 GO:0016101 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0019432 GO:0033306 GO:0034308 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:1901576 GO:1901615 GO:1903173

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6 DAGAT Hydrolase_4

Protein Analysis

662

Amino Acids

73.98

Weight (kDa)

8.8

Isoelectric Point (pI)

40.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_1	PF00561	92 - 337	2.7e-08	alpha/beta hydrolase fold
Hydrolase_4	PF12146	131 - 336	6.1e-10	Serine aminopeptidase, S33
Abhydrolase_6	PF12697	131 - 340	1.9e-08	Alpha/beta hydrolase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000740)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02030 AT3G02030 AT3G26820 AT3G26820 AT3G26840 AT5G41120 AT5G41120 AT5G41120 AT5G41130 AT5G41130 AT5G41130
fragaria_vesca	FvH4_3g35400 FvH4_3g39220 FvH4_3g39220 FvH4_3g39270 FvH4_3g39280 FvH4_3g39280 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060
malus_domestica	MD09G1005800.v1.1 MD17G1009900.v1.1
prunus_persica	Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1
pyrus_communis	pycom111g00420
rosa_chinensis	RchiOBHm_Chr2g0176241 RchiOBHm_Chr5g0070671 RchiOBHm_Chr5g0070681 RchiOBHm_Chr5g0070691
rosa_laevigata	RLG00000022394 RLG00000036149 RLG00000036150 RLG00000036152
rosa_multiflora	Rmu_co8466433.1_g000001 Rmu_sc0000249.1_g000001 Rmu_sc0000405.1_g000016 Rmu_sc0005197.1_g000009 Rmu_sc0005197.1_g000015 Rmu_sc0036323.1_g000001
rosa_roxburghii	Rroxscaffold_1G00010480 Rroxscaffold_1G00010530 Rroxscaffold_1G00010550 Rroxscaffold_1G00010560 Rroxscaffold_2G00076650 Rroxscaffold_5G00357250
rosa_rugosa	Rorug02G0592200 Rorug02G0592300 Rorug05G0407500 Rorug05G0407800
rosa_samantha	Rh2AG670700 Rh2BG682200 Rh2CG645000 Rh2DG695800 Rh5AG463200 Rh5AG463300 Rh5AG463400 Rh5BG481200 Rh5CG505400 Rh5CG505500 Rh5DG493300 Rh5DG493600 Rh5DG493700
rosa_wichuraiana	Rw2G055020 Rw5G043110 Rw5G043120 Rw5G043130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1953
AbsI	CCTCGAGG	1 cut(s)	179
Acc36I	ACCTGC	1 cut(s)	1953
AccB1I	GGYRCC	2 cut(s)	269, 491
AccBSI	CCGCTC	2 cut(s)	277, 972
AccI	GTMKAC	2 cut(s)	1172, 1617
AccII	CGCG	1 cut(s)	1488
AccIII	TCCGGA	1 cut(s)	712
AclWI	GGATC	5 cut(s)	251, 264, 1136, 1901, 1914
AcoI	YGGCCR	3 cut(s)	6, 988, 1517
AcsI	RAATTY	2 cut(s)	1212, 1553
AcyI	GRCGYC	1 cut(s)	492
AfaI	GTAC	3 cut(s)	870, 1233, 1844
AgsI	TTSAA	4 cut(s)	46, 422, 743, 979
AjiI	CACGTC	1 cut(s)	1852
AjnI	CCWGG	9 cut(s)	292, 454, 603, 664, 1282, 1474, 1701, 1738, 1759
AloI	GAACNNNNNNTCC	2 cut(s)	1278, 1310
Alw21I	GWGCWC	1 cut(s)	1332
Alw26I	GTCTC	7 cut(s)	710, 1068, 1084, 1342, 1812, 1818, 1937
Alw44I	GTGCAC	1 cut(s)	1328
AlwI	GGATC	5 cut(s)	251, 264, 1136, 1901, 1914
Ama87I	CYCGRG	4 cut(s)	127, 179, 314, 1420
Aor13HI	TCCGGA	1 cut(s)	712
AoxI	GGCC	9 cut(s)	6, 217, 670, 988, 1156, 1319, 1517, 1681, 1954
ApaI	GGGCCC	1 cut(s)	1160
ApaLI	GTGCAC	1 cut(s)	1328
ApeKI	GCWGC	9 cut(s)	148, 328, 515, 586, 728, 836, 1544, 1547, 1948
ApoI	RAATTY	2 cut(s)	1212, 1553
Asp700I	GAANNNNTTC	1 cut(s)	804
AspLEI	GCGC	1 cut(s)	494
AspS9I	GGNCC	7 cut(s)	218, 671, 1156, 1157, 1226, 1411, 1682
AsuC2I	CCSGG	2 cut(s)	89, 1685
AsuHPI	GGTGA	9 cut(s)	106, 444, 763, 1159, 1207, 1421, 1660, 1702, 1934
AvaI	CYCGRG	4 cut(s)	127, 179, 314, 1420
AvaII	GGWCC	2 cut(s)	1226, 1411
BaeGI	GKGCMC	4 cut(s)	274, 1160, 1332, 1427
BaeI	ACNNNNGTAYC	2 cut(s)	1739, 1772
BalI	TGGCCA	1 cut(s)	1519
BamHI	GGATCC	2 cut(s)	256, 1906
BanI	GGYRCC	2 cut(s)	269, 491
BanII	GRGCYC	1 cut(s)	1160
BarI	GAAGNNNNNNTAC	2 cut(s)	1215, 1247
BauI	CACGAG	1 cut(s)	1298
BbsI	GAAGAC	1 cut(s)	216
Bbv12I	GWGCWC	1 cut(s)	1332
BbvI	GCAGC	9 cut(s)	160, 340, 527, 573, 715, 823, 1531, 1559, 1960
BccI	CCATC	8 cut(s)	229, 679, 1109, 1178, 1225, 1352, 1396, 1424
BceAI	ACGGC	5 cut(s)	17, 232, 1003, 1027, 1969
BcgI	CGANNNNNNTGC	2 cut(s)	487, 521
BciT130I	CCWGG	9 cut(s)	294, 456, 605, 666, 1284, 1476, 1703, 1740, 1761
BclI	TGATCA	1 cut(s)	1149
BcnI	CCSGG	2 cut(s)	89, 1685
BcoDI	GTCTC	7 cut(s)	710, 1068, 1084, 1342, 1812, 1818, 1937
BfaI	CTAG	3 cut(s)	407, 779, 866
BfmI	CTRYAG	2 cut(s)	1545, 1946
BfoI	RGCGCY	1 cut(s)	495
BfuAI	ACCTGC	1 cut(s)	1953
BglII	AGATCT	1 cut(s)	403
BlpI	GCTNAGC	1 cut(s)	653
Bme18I	GGWCC	2 cut(s)	1226, 1411
BmeT110I	CYCGRG	4 cut(s)	127, 179, 314, 1420
BmgBI	CACGTC	1 cut(s)	1852
BmgT120I	GGNCC	7 cut(s)	218, 671, 1156, 1157, 1226, 1411, 1682
BmiI	GGNNCC	9 cut(s)	258, 271, 493, 1158, 1413, 1561, 1641, 1721, 1908
BmrI	ACTGGG	2 cut(s)	706, 912
BmsI	GCATC	3 cut(s)	820, 1491, 1862
BmuI	ACTGGG	2 cut(s)	706, 912
BpiI	GAAGAC	1 cut(s)	216
BplI	GAGNNNNNCTC	4 cut(s)	471, 503, 787, 819
BpmI	CTGGAG	1 cut(s)	1025
Bpu10I	CCTNAGC	2 cut(s)	189, 1394
Bpu1102I	GCTNAGC	1 cut(s)	653
BpuEI	CTTGAG	2 cut(s)	803, 1163
BpuMI	CCSGG	2 cut(s)	89, 1685
BsaBI	GATNNNNATC	1 cut(s)	534
BsaHI	GRCGYC	1 cut(s)	492
BsaI	GGTCTC	1 cut(s)	1068
BsaJI	CCNNGG	7 cut(s)	70, 87, 414, 455, 603, 1282, 1760
BsaWI	WCCGGW	3 cut(s)	11, 205, 712
Bse118I	RCCGGY	1 cut(s)	1053
Bse1I	ACTGG	3 cut(s)	701, 918, 1263
Bse3DI	GCAATG	2 cut(s)	498, 991
Bse8I	GATNNNNATC	1 cut(s)	534
BseAI	TCCGGA	1 cut(s)	712
BseBI	CCWGG	9 cut(s)	294, 456, 605, 666, 1284, 1476, 1703, 1740, 1761
BseDI	CCNNGG	7 cut(s)	70, 87, 414, 455, 603, 1282, 1760
BseGI	GGATG	5 cut(s)	690, 758, 1089, 1404, 1482
BseJI	GATNNNNATC	1 cut(s)	534
BseMI	GCAATG	2 cut(s)	498, 991
BseMII	CTCAG	4 cut(s)	890, 1098, 1343, 1408
BseNI	ACTGG	3 cut(s)	701, 918, 1263
BseRI	GAGGAG	2 cut(s)	1916, 1949
BseSI	GKGCMC	4 cut(s)	274, 1160, 1332, 1427
BseXI	GCAGC	9 cut(s)	160, 340, 527, 573, 715, 823, 1531, 1559, 1960
BseYI	CCCAGC	1 cut(s)	559
Bsh1236I	CGCG	1 cut(s)	1488
BshFI	GGCC	9 cut(s)	8, 219, 672, 990, 1158, 1321, 1519, 1683, 1956
BshNI	GGYRCC	2 cut(s)	269, 491
BsiHKAI	GWGCWC	1 cut(s)	1332
BsiHKCI	CYCGRG	4 cut(s)	127, 179, 314, 1420
BsiSI	CCGG	6 cut(s)	12, 89, 206, 713, 1054, 1685
BslFI	GGGAC	3 cut(s)	1397, 1556, 1955
BsmAI	GTCTC	7 cut(s)	710, 1068, 1084, 1342, 1812, 1818, 1937
BsmBI	CGTCTC	3 cut(s)	710, 1084, 1937
BsmFI	GGGAC	3 cut(s)	1397, 1556, 1955
BsmI	GAATGC	1 cut(s)	577
BsnI	GGCC	9 cut(s)	8, 219, 672, 990, 1158, 1321, 1519, 1683, 1956
Bso31I	GGTCTC	1 cut(s)	1068
BsoBI	CYCGRG	4 cut(s)	127, 179, 314, 1420
Bsp120I	GGGCCC	1 cut(s)	1156
Bsp1286I	GDGCHC	4 cut(s)	274, 1160, 1332, 1427
Bsp13I	TCCGGA	1 cut(s)	712
Bsp143I	GATC	8 cut(s)	256, 403, 449, 535, 1018, 1128, 1149, 1906
Bsp1720I	GCTNAGC	1 cut(s)	653
Bsp19I	CCATGG	1 cut(s)	414
BspANI	GGCC	9 cut(s)	8, 219, 672, 990, 1158, 1321, 1519, 1683, 1956
BspCNI	CTCAG	4 cut(s)	889, 1099, 1344, 1407
BspEI	TCCGGA	1 cut(s)	712
BspFNI	CGCG	1 cut(s)	1488
BspLI	GGNNCC	9 cut(s)	258, 271, 493, 1158, 1413, 1561, 1641, 1721, 1908
BspMAI	CTGCAG	2 cut(s)	1549, 1950
BspMI	ACCTGC	1 cut(s)	1953
BspPI	GGATC	5 cut(s)	251, 264, 1136, 1901, 1914
BspQI	GCTCTTC	1 cut(s)	397
BspT107I	GGYRCC	2 cut(s)	269, 491
BspTNI	GGTCTC	1 cut(s)	1068
BsrBI	CCGCTC	2 cut(s)	277, 972
BsrDI	GCAATG	2 cut(s)	498, 991
BsrFI	RCCGGY	1 cut(s)	1053
BsrI	ACTGG	3 cut(s)	701, 918, 1263
BssAI	RCCGGY	1 cut(s)	1053
BssECI	CCNNGG	7 cut(s)	70, 87, 414, 455, 603, 1282, 1760
BssMI	GATC	8 cut(s)	256, 403, 449, 535, 1018, 1128, 1149, 1906
BssNI	GRCGYC	1 cut(s)	492
BssSI	CACGAG	1 cut(s)	1298
BssT1I	CCWWGG	2 cut(s)	70, 414
Bst2BI	CACGAG	1 cut(s)	1298
Bst2UI	CCWGG	9 cut(s)	294, 456, 605, 666, 1284, 1476, 1703, 1740, 1761
Bst4CI	ACNGT	4 cut(s)	118, 269, 430, 1236
Bst6I	CTCTTC	5 cut(s)	290, 397, 810, 1707, 1803
BstACI	GRCGYC	1 cut(s)	492
BstC8I	GCNNGC	4 cut(s)	94, 368, 382, 496
BstDEI	CTNAG	6 cut(s)	189, 653, 876, 1107, 1352, 1394
BstDSI	CCRYGG	1 cut(s)	414
BstF5I	GGATG	5 cut(s)	690, 758, 1089, 1404, 1482
BstFNI	CGCG	1 cut(s)	1488
BstH2I	RGCGCY	1 cut(s)	495
BstHHI	GCGC	1 cut(s)	494
BstKTI	GATC	8 cut(s)	259, 406, 452, 538, 1021, 1131, 1152, 1909
BstMAI	GTCTC	7 cut(s)	710, 1068, 1084, 1342, 1812, 1818, 1937
BstMBI	GATC	8 cut(s)	256, 403, 449, 535, 1018, 1128, 1149, 1906
BstMWI	GCNNNNNNNGC	8 cut(s)	225, 325, 500, 515, 583, 823, 845, 1327
BstNI	CCWGG	9 cut(s)	294, 456, 605, 666, 1284, 1476, 1703, 1740, 1761
BstNSI	RCATGY	3 cut(s)	1255, 1393, 1738
BstSFI	CTRYAG	2 cut(s)	1545, 1946
BstSLI	GKGCMC	4 cut(s)	274, 1160, 1332, 1427
BstUI	CGCG	1 cut(s)	1488
BstV1I	GCAGC	9 cut(s)	160, 340, 527, 573, 715, 823, 1531, 1559, 1960
BstV2I	GAAGAC	1 cut(s)	216
BstX2I	RGATCY	3 cut(s)	256, 403, 1906
BstXI	CCANNNNNNTGG	2 cut(s)	228, 341
BstYI	RGATCY	3 cut(s)	256, 403, 1906
BsuRI	GGCC	9 cut(s)	8, 219, 672, 990, 1158, 1321, 1519, 1683, 1956
BtgI	CCRYGG	1 cut(s)	414
BtrI	CACGTC	1 cut(s)	1852
BtsCI	GGATG	5 cut(s)	690, 758, 1089, 1404, 1482
BtsIMutI	CAGTG	1 cut(s)	694
BveI	ACCTGC	1 cut(s)	1953
Cac8I	GCNNGC	4 cut(s)	94, 368, 382, 496
CfoI	GCGC	1 cut(s)	494
Cfr10I	RCCGGY	1 cut(s)	1053
Cfr13I	GGNCC	7 cut(s)	218, 671, 1156, 1157, 1226, 1411, 1682
CsiI	ACCWGGT	1 cut(s)	292
Csp6I	GTAC	3 cut(s)	869, 1232, 1843
CviAII	CATG	7 cut(s)	261, 415, 1153, 1252, 1336, 1390, 1735
CviQI	GTAC	3 cut(s)	869, 1232, 1843
DdeI	CTNAG	6 cut(s)	189, 653, 876, 1107, 1352, 1394
DinI	GGCGCC	1 cut(s)	493
DpnI	GATC	8 cut(s)	258, 405, 451, 537, 1020, 1130, 1151, 1908
DpnII	GATC	8 cut(s)	256, 403, 449, 535, 1018, 1128, 1149, 1906
DraI	TTTAAA	2 cut(s)	678, 1605
EaeI	YGGCCR	3 cut(s)	6, 988, 1517
Eam1104I	CTCTTC	5 cut(s)	290, 397, 810, 1707, 1803
EarI	CTCTTC	5 cut(s)	290, 397, 810, 1707, 1803
Eco130I	CCWWGG	2 cut(s)	70, 414
Eco24I	GRGCYC	1 cut(s)	1160
Eco31I	GGTCTC	1 cut(s)	1068
Eco32I	GATATC	1 cut(s)	358
Eco47I	GGWCC	2 cut(s)	1226, 1411
Eco88I	CYCGRG	4 cut(s)	127, 179, 314, 1420
EcoO109I	RGGNCCY	2 cut(s)	1157, 1226
EcoRI	GAATTC	1 cut(s)	1212
EcoRII	CCWGG	9 cut(s)	292, 454, 603, 664, 1282, 1474, 1701, 1738, 1759
EcoRV	GATATC	1 cut(s)	358
EcoT14I	CCWWGG	2 cut(s)	70, 414
EcoT22I	ATGCAT	1 cut(s)	1877
EcoT38I	GRGCYC	1 cut(s)	1160
EgeI	GGCGCC	1 cut(s)	493
EheI	GGCGCC	1 cut(s)	493
ErhI	CCWWGG	2 cut(s)	70, 414
Esp3I	CGTCTC	3 cut(s)	710, 1084, 1937
FaeI	CATG	7 cut(s)	264, 418, 1156, 1255, 1339, 1393, 1738
FaqI	GGGAC	3 cut(s)	1397, 1556, 1955
FatI	CATG	7 cut(s)	260, 414, 1152, 1251, 1335, 1389, 1734
FauI	CCCGC	1 cut(s)	282
FbaI	TGATCA	1 cut(s)	1149
FblI	GTMKAC	2 cut(s)	1172, 1617
FokI	GGATG	5 cut(s)	697, 745, 1076, 1411, 1469
FriOI	GRGCYC	1 cut(s)	1160
FspBI	CTAG	3 cut(s)	407, 779, 866
GlaI	GCGC	1 cut(s)	493
GsaI	CCCAGC	1 cut(s)	563
GsuI	CTGGAG	1 cut(s)	1025
HaeII	RGCGCY	1 cut(s)	495
HaeIII	GGCC	9 cut(s)	8, 219, 672, 990, 1158, 1321, 1519, 1683, 1956
HapII	CCGG	6 cut(s)	12, 89, 206, 713, 1054, 1685
HhaI	GCGC	1 cut(s)	494
Hin1I	GRCGYC	1 cut(s)	492
Hin1II	CATG	7 cut(s)	264, 418, 1156, 1255, 1339, 1393, 1738
Hin6I	GCGC	1 cut(s)	492
HinP1I	GCGC	1 cut(s)	492
HincII	GTYRAC	1 cut(s)	1173
HindII	GTYRAC	1 cut(s)	1173
HindIII	AAGCTT	1 cut(s)	655
HinfI	GANTC	6 cut(s)	99, 312, 482, 547, 661, 880
HpaII	CCGG	6 cut(s)	12, 89, 206, 713, 1054, 1685
HphI	GGTGA	9 cut(s)	106, 444, 763, 1159, 1207, 1421, 1660, 1702, 1934
Hpy166II	GTNNAC	6 cut(s)	139, 1173, 1297, 1330, 1618, 1843
Hpy188I	TCNGA	9 cut(s)	38, 203, 704, 795, 879, 945, 1084, 1108, 1222
Hpy8I	GTNNAC	6 cut(s)	139, 1173, 1297, 1330, 1618, 1843
Hpy99I	CGWCG	3 cut(s)	38, 305, 1177
HpyAV	CCTTC	9 cut(s)	290, 654, 923, 1217, 1673, 1700, 1780, 1852, 1890
HpyCH4III	ACNGT	4 cut(s)	118, 269, 430, 1236
HpyCH4IV	ACGT	2 cut(s)	1077, 1851
HpyF10VI	GCNNNNNNNGC	8 cut(s)	225, 325, 500, 515, 583, 823, 845, 1327
HpyF3I	CTNAG	6 cut(s)	189, 653, 876, 1107, 1352, 1394
HpySE526I	ACGT	2 cut(s)	1077, 1851
Hsp92I	GRCGYC	1 cut(s)	492
Hsp92II	CATG	7 cut(s)	264, 418, 1156, 1255, 1339, 1393, 1738
HspAI	GCGC	1 cut(s)	492
KasI	GGCGCC	1 cut(s)	491
Kpn2I	TCCGGA	1 cut(s)	712
Ksp22I	TGATCA	1 cut(s)	1149
Kzo9I	GATC	8 cut(s)	256, 403, 449, 535, 1018, 1128, 1149, 1906
LguI	GCTCTTC	1 cut(s)	397
LmnI	GCTCC	4 cut(s)	282, 604, 1559, 1802
Lsp1109I	GCAGC	9 cut(s)	160, 340, 527, 573, 715, 823, 1531, 1559, 1960
LweI	GCATC	3 cut(s)	820, 1491, 1862
MabI	ACCWGGT	1 cut(s)	292
MaeI	CTAG	3 cut(s)	407, 779, 866
MaeII	ACGT	2 cut(s)	1077, 1851
MaeIII	GTNAC	7 cut(s)	76, 112, 263, 521, 1265, 1365, 1669
MalI	GATC	8 cut(s)	258, 405, 451, 537, 1020, 1130, 1151, 1908
MbiI	CCGCTC	2 cut(s)	277, 972
MboI	GATC	8 cut(s)	256, 403, 449, 535, 1018, 1128, 1149, 1906
MflI	RGATCY	3 cut(s)	256, 403, 1906
MhlI	GDGCHC	4 cut(s)	274, 1160, 1332, 1427
MlsI	TGGCCA	1 cut(s)	1519
MluCI	AATT	5 cut(s)	885, 1212, 1289, 1312, 1553
MluNI	TGGCCA	1 cut(s)	1519
Mly113I	GGCGCC	1 cut(s)	492
MlyI	GAGTC	2 cut(s)	306, 874
MmeI	TCCRAC	3 cut(s)	16, 457, 1107
Mox20I	TGGCCA	1 cut(s)	1519
Mph1103I	ATGCAT	1 cut(s)	1877
MroI	TCCGGA	1 cut(s)	712
MroXI	GAANNNNTTC	1 cut(s)	804
MscI	TGGCCA	1 cut(s)	1519
MseI	TTAA	8 cut(s)	159, 609, 630, 677, 767, 957, 1113, 1604
Msp20I	TGGCCA	1 cut(s)	1519
MspA1I	CMGCKG	4 cut(s)	728, 836, 897, 1676
MspI	CCGG	6 cut(s)	12, 89, 206, 713, 1054, 1685
Mva1269I	GAATGC	1 cut(s)	577
MvaI	CCWGG	9 cut(s)	294, 456, 605, 666, 1284, 1476, 1703, 1740, 1761
MvnI	CGCG	1 cut(s)	1488
MwoI	GCNNNNNNNGC	8 cut(s)	225, 325, 500, 515, 583, 823, 845, 1327
NarI	GGCGCC	1 cut(s)	492
NciI	CCSGG	2 cut(s)	89, 1685
NcoI	CCATGG	1 cut(s)	414
NdeII	GATC	8 cut(s)	256, 403, 449, 535, 1018, 1128, 1149, 1906
NlaIII	CATG	7 cut(s)	264, 418, 1156, 1255, 1339, 1393, 1738
NlaIV	GGNNCC	9 cut(s)	258, 271, 493, 1158, 1413, 1561, 1641, 1721, 1908
NmeAIII	GCCGAG	1 cut(s)	468
NmuCI	GTSAC	4 cut(s)	112, 263, 1265, 1669
NsiI	ATGCAT	1 cut(s)	1877
NspI	RCATGY	3 cut(s)	1255, 1393, 1738
PaeR7I	CTCGAG	2 cut(s)	127, 179
PaqCI	CACCTGC	1 cut(s)	1953
PciSI	GCTCTTC	1 cut(s)	397
PctI	GAATGC	1 cut(s)	577
PdmI	GAANNNNTTC	1 cut(s)	804
PfeI	GAWTC	4 cut(s)	99, 482, 547, 661
PfoI	TCCNGGA	1 cut(s)	1474
PleI	GAGTC	2 cut(s)	306, 874
PluTI	GGCGCC	1 cut(s)	495
PpsI	GAGTC	2 cut(s)	306, 874
PpuMI	RGGWCCY	1 cut(s)	1226
Psp5II	RGGWCCY	1 cut(s)	1226
Psp6I	CCWGG	9 cut(s)	292, 454, 603, 664, 1282, 1474, 1701, 1738, 1759
PspFI	CCCAGC	1 cut(s)	559
PspGI	CCWGG	9 cut(s)	292, 454, 603, 664, 1282, 1474, 1701, 1738, 1759
PspN4I	GGNNCC	9 cut(s)	258, 271, 493, 1158, 1413, 1561, 1641, 1721, 1908
PspOMI	GGGCCC	1 cut(s)	1156
PspPI	GGNCC	7 cut(s)	218, 671, 1156, 1157, 1226, 1411, 1682
PspPPI	RGGWCCY	1 cut(s)	1226
PspXI	VCTCGAGB	2 cut(s)	127, 179
PstI	CTGCAG	2 cut(s)	1549, 1950
PsuI	RGATCY	3 cut(s)	256, 403, 1906
PvuII	CAGCTG	1 cut(s)	728
RsaI	GTAC	3 cut(s)	870, 1233, 1844
RsaNI	GTAC	3 cut(s)	869, 1232, 1843
SalI	GTCGAC	1 cut(s)	1171
SapI	GCTCTTC	1 cut(s)	397
SaqAI	TTAA	8 cut(s)	159, 609, 630, 677, 767, 957, 1113, 1604
Sau3AI	GATC	8 cut(s)	256, 403, 449, 535, 1018, 1128, 1149, 1906
Sau96I	GGNCC	7 cut(s)	218, 671, 1156, 1157, 1226, 1411, 1682
SchI	GAGTC	2 cut(s)	306, 874
SduI	GDGCHC	4 cut(s)	274, 1160, 1332, 1427
SexAI	ACCWGGT	1 cut(s)	292
SfaNI	GCATC	3 cut(s)	820, 1491, 1862
SfcI	CTRYAG	2 cut(s)	1545, 1946
SfoI	GGCGCC	1 cut(s)	493
Sfr274I	CTCGAG	2 cut(s)	127, 179
SinI	GGWCC	2 cut(s)	1226, 1411
SlaI	CTCGAG	2 cut(s)	127, 179
SmlI	CTYRAG	4 cut(s)	127, 179, 818, 1142
SmoI	CTYRAG	4 cut(s)	127, 179, 818, 1142
Sse9I	AATT	5 cut(s)	885, 1212, 1289, 1312, 1553
SspDI	GGCGCC	1 cut(s)	491
SspMI	CTAG	3 cut(s)	407, 779, 866
StyI	CCWWGG	2 cut(s)	70, 414
TaaI	ACNGT	4 cut(s)	118, 269, 430, 1236
TaiI	ACGT	2 cut(s)	1080, 1854
TaqI	TCGA	7 cut(s)	128, 180, 243, 300, 1172, 1346, 1866
TaqII	GACCGA	1 cut(s)	1399
TasI	AATT	5 cut(s)	885, 1212, 1289, 1312, 1553
TatI	WGTACW	2 cut(s)	868, 1231
TauI	GCSGC	4 cut(s)	8, 188, 836, 1321
TfiI	GAWTC	4 cut(s)	99, 482, 547, 661
Tru1I	TTAA	8 cut(s)	159, 609, 630, 677, 767, 957, 1113, 1604
Tru9I	TTAA	8 cut(s)	159, 609, 630, 677, 767, 957, 1113, 1604
TscAI	CASTG	1 cut(s)	701
TseFI	GTSAC	4 cut(s)	112, 263, 1265, 1669
TseI	GCWGC	9 cut(s)	148, 328, 515, 586, 728, 836, 1544, 1547, 1948
Tsp45I	GTSAC	4 cut(s)	112, 263, 1265, 1669
TspDTI	ATGAA	3 cut(s)	187, 453, 942
TspGWI	ACGGA	5 cut(s)	63, 318, 637, 734, 1632
TspRI	CASTG	1 cut(s)	701
VneI	GTGCAC	1 cut(s)	1328
VpaK11BI	GGWCC	2 cut(s)	1226, 1411
XapI	RAATTY	2 cut(s)	1212, 1553
XceI	RCATGY	3 cut(s)	1255, 1393, 1738
XhoI	CTCGAG	2 cut(s)	127, 179
XmiI	GTMKAC	2 cut(s)	1172, 1617
XmnI	GAANNNNTTC	1 cut(s)	804
XspI	CTAG	3 cut(s)	407, 779, 866
Zsp2I	ATGCAT	1 cut(s)	1877

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.