Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0000740

Physical Location & Seq

Genomic Coordinates Reverse (-)

81541101 .. 81547069

Gene Structure 5969 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG645000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1881 bp

ATGGCCTCCAACGCACTCTCTCTCCTCCCCTCCTGCTGCCGCTCCCCGTCCATTTTCCGACATGAACCGACGTCTTCACCATTACTATCCACAAGGTCCAAGCTGGCTAGGACCACCAGATTGGCCATTTCCACAGACCAAACTCCAGTCTTCACGACGTCAACGGCAACTGCTTTGTCAGAGTCAGACAAGTCCAAGATCAAGACCGAGGAAGAGCACTTGGCTCTGGAGGACGACGTTGGTGATGATCAAGCATCGGAAGAGCGTGCGAGCTTAAACCAGTTCTTTGAGCAAGCCAAGGGTCTTCTCAGATCGGACGACCAGTACGGGCAGTACGGCACTGGAACACCTCGCTGGTTCTCGCCTTTAGACTGCGGGAGCGGGACCCCCTCCAACCATGATTCCCCGCTCCTTCTCTTTTTGCCTGGAATTGATGGCACCGGACTTGGCCTTGTAAGGCATCATCAGAAACTTGGAAAGATTTTTGAGGTCTCGTGCTTGCATATCCCTCCTAAGGATCGAACACCGTTTATAGACTTAGTGAAGCTGGTTGGGAGAACAATTCGTTCAGAGCATGATGCCTCACCCGATCGTCCCATATATCTTGTTGGAGAATCTCTTGGAGCATGCCTTGCACTCTCTGTGGCAGCCCTTAACCCGGATATCGACCTTGAGCTTATTTTAGCAAATCCAGCAACATCCTTCAGCAAGTCACCACTTCAAGCTGTATTACCTTTACTGCAGTTCATGCCTGACTCTCCCAATCTTAGCCTTCCTCATATACTGAGTACAATTACAGGTGCAATGGTGGCTAGTCTGGAGAAGGGAGTTGGAGGGCTTCCACTGCCACAGACAGTTGGAAAGCTATCACGGGATATTATTGAACCATCAATATCTAACCTTTCTGTTCTTGCTGATATCTTACCCAGAGAAACACTTCTCTGGAAGCTACAAATGATCAGTACAGCTTCTACATATGCCAATTCACGCCTCCATGCTGTAAAAGCTCATACCGTGATAGTATCCAGTGGAAGAGATCCATTGCTACCTAGTCAAGAGGAAGGTCAAAGACTCAAAAGCCTGCTTGCAACTTGTCAGGTCCGTACGTTTGAAGACTGCGGCCATTTCCTCTTCTTGGAAGATGCTTTTGATTTGCTAACTGTCATCAAGAATGTTGGTATCTATCGTCGTTCAAAAGAACGAGACTTTGTTTCAGATTACATACCACCATCACCTTCCGAAGTTAAAGGCATAATGGAAAAAAACAGATTGGTTAACATAATCATGAGCCCAGTGATGCTCTCAACTTTAGCAGACGGAAAGATAGTGAGGGGCTTTGCCGGGATTCCTACAGAGGGTCCTGTATTGTATGTTGGTTATCACATGTTACTGGGATTTGAAATAGTTCCCTTGGTGACTCAACTGTTCGAACAAAAAAACGTCCATCTGCGAGGGATAGCACATCCAATGATGTTTCTCAAAACGAAAAATGGAAGTTTACCTGAGTTAACATTATACGATGATTTCAGACTTATGGGCGCTGTTCCTGTCTCGGGAAAAAACTTTTTCAAACTTCTCTCTACAAAGTCTCATGTCCTACTCTATCCAGGTGGAGTGCGTGAGGCTCTTCACCGGAAGGGGGAAGCATATAAGTTGTTCTGGCCGGAACGGTCAGAATTTGTGAGGATGGCAGCTAGATTTGGAGCCAAAATCGTACCTTTTGGTGCTGTTGGAGAAGATGATATTGGTGATGTAAGGCTACAAACCTCACTTTATCTTTTGTATTTAGTAATATGCCTTTCCATTAAATATTTATTGAAGCTTGAAGTAGGTGTTACTGTGTTATCAAAATTACTCTTATACCGGATTGTGGTTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010287 GO:0016101 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0019432 GO:0033306 GO:0034308 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:1901576 GO:1901615 GO:1903173

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6 DAGAT Hydrolase_4

Protein Analysis

626

Amino Acids

68.79

Weight (kDa)

7.26

Isoelectric Point (pI)

41.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_1	PF00561	174 - 381	5.2e-09	alpha/beta hydrolase fold
Abhydrolase_6	PF12697	177 - 384	4.8e-08	Alpha/beta hydrolase family
Hydrolase_4	PF12146	193 - 380	2.8e-07	Serine aminopeptidase, S33
DAGAT	PF03982	506 - 582	7.4e-09	Diacylglycerol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000740)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02030 AT3G02030 AT3G26820 AT3G26820 AT3G26840 AT5G41120 AT5G41120 AT5G41120 AT5G41130 AT5G41130 AT5G41130
fragaria_vesca	FvH4_3g35400 FvH4_3g39220 FvH4_3g39220 FvH4_3g39270 FvH4_3g39280 FvH4_3g39280 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060
malus_domestica	MD09G1005800.v1.1 MD17G1009900.v1.1
prunus_persica	Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1
pyrus_communis	pycom111g00420
rosa_chinensis	RchiOBHm_Chr2g0176241 RchiOBHm_Chr5g0070671 RchiOBHm_Chr5g0070681 RchiOBHm_Chr5g0070691
rosa_laevigata	RLG00000022394 RLG00000036149 RLG00000036150 RLG00000036152
rosa_multiflora	Rmu_co8466433.1_g000001 Rmu_sc0000249.1_g000001 Rmu_sc0000405.1_g000016 Rmu_sc0005197.1_g000009 Rmu_sc0005197.1_g000015 Rmu_sc0036323.1_g000001
rosa_roxburghii	Rroxscaffold_1G00010480 Rroxscaffold_1G00010530 Rroxscaffold_1G00010550 Rroxscaffold_1G00010560 Rroxscaffold_2G00076650 Rroxscaffold_5G00357250
rosa_rugosa	Rorug02G0592200 Rorug02G0592300 Rorug05G0407500 Rorug05G0407800
rosa_samantha	Rh2AG670700 Rh2BG682200 Rh2CG645000 Rh2DG695800 Rh5AG463200 Rh5AG463300 Rh5AG463400 Rh5BG481200 Rh5CG505400 Rh5CG505500 Rh5DG493300 Rh5DG493600 Rh5DG493700
rosa_wichuraiana	Rw2G055020 Rw5G043110 Rw5G043120 Rw5G043130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	74, 161
AccB1I	GGYRCC	1 cut(s)	437
AccBSI	CCGCTC	3 cut(s)	42, 381, 409
AciI	CCGC	5 cut(s)	40, 375, 381, 407, 1119
AclWI	GGATC	2 cut(s)	525, 1031
AcoI	YGGCCR	3 cut(s)	123, 1120, 1661
AcsI	RAATTY	1 cut(s)	1676
AcuI	CTGAAG	1 cut(s)	688
AcyI	GRCGYC	2 cut(s)	71, 158
AfaI	GTAC	6 cut(s)	326, 335, 790, 964, 1105, 1716
AfiI	CCNNNNNNNGG	7 cut(s)	514, 658, 1135, 1355, 1553, 1639, 1870
AflIII	ACRYGT	1 cut(s)	1383
AgsI	TTSAA	8 cut(s)	722, 884, 1112, 1194, 1400, 1570, 1819, 1826
AjnI	CCWGG	2 cut(s)	424, 1606
AjuI	GAANNNNNNNTTGG	2 cut(s)	1728, 1760
Alw21I	GWGCWC	1 cut(s)	219
Alw26I	GTCTC	4 cut(s)	496, 1197, 1555, 1593
AlwI	GGATC	2 cut(s)	525, 1031
Ama87I	CYCGRG	1 cut(s)	1552
AoxI	GGCC	5 cut(s)	3, 123, 448, 1120, 1661
ApeKI	GCWGC	3 cut(s)	36, 647, 1691
ApoI	RAATTY	1 cut(s)	1676
Asp700I	GAANNNNTTC	2 cut(s)	936, 1404
AspLEI	GCGC	1 cut(s)	1541
AspS9I	GGNCC	5 cut(s)	96, 111, 384, 1099, 1358
AsuC2I	CCSGG	2 cut(s)	659, 1342
AsuHPI	GGTGA	8 cut(s)	69, 254, 576, 705, 1224, 1426, 1622, 1760
AsuII	TTCGAA	1 cut(s)	1428
AvaI	CYCGRG	1 cut(s)	1552
AvaII	GGWCC	5 cut(s)	96, 111, 384, 1099, 1358
AxyI	CCTNAGG	1 cut(s)	513
BalI	TGGCCA	1 cut(s)	125
BanI	GGYRCC	1 cut(s)	437
BanII	GRGCYC	1 cut(s)	1292
BauI	CACGAG	1 cut(s)	493
BbsI	GAAGAC	4 cut(s)	66, 142, 296, 1119
Bbv12I	GWGCWC	1 cut(s)	219
BbvI	GCAGC	3 cut(s)	23, 659, 1703
BccI	CCATC	5 cut(s)	428, 895, 1237, 1452, 1681
BceAI	ACGGC	2 cut(s)	180, 352
BciT130I	CCWGG	2 cut(s)	426, 1608
BciVI	GTATCC	1 cut(s)	1033
BclI	TGATCA	2 cut(s)	247, 957
BcnI	CCSGG	2 cut(s)	659, 1342
BcoDI	GTCTC	4 cut(s)	496, 1197, 1555, 1593
BfaI	CTAG	4 cut(s)	108, 813, 1050, 1695
BfmI	CTRYAG	2 cut(s)	740, 1350
BfoI	RGCGCY	1 cut(s)	1542
BfuI	GTATCC	1 cut(s)	1033
BisI	GCNGC	5 cut(s)	37, 40, 648, 1120, 1692
BlsI	GCNGC	5 cut(s)	38, 41, 649, 1121, 1693
Bme1390I	CCNGG	4 cut(s)	426, 659, 1342, 1608
Bme18I	GGWCC	5 cut(s)	96, 111, 384, 1099, 1358
BmeT110I	CYCGRG	1 cut(s)	1552
BmgT120I	GGNCC	5 cut(s)	96, 111, 384, 1099, 1358
BmiI	GGNNCC	5 cut(s)	385, 386, 439, 1359, 1705
BmrFI	CCNGG	4 cut(s)	426, 659, 1342, 1608
BmrI	ACTGGG	2 cut(s)	1286, 1400
BmsI	GCATC	5 cut(s)	263, 469, 568, 1132, 1287
BmuI	ACTGGG	2 cut(s)	1286, 1400
BpiI	GAAGAC	4 cut(s)	66, 142, 296, 1119
BpmI	CTGGAG	3 cut(s)	129, 248, 839
Bpu14I	TTCGAA	1 cut(s)	1428
BpuEI	CTTGAG	1 cut(s)	692
BpuMI	CCSGG	2 cut(s)	659, 1342
BsaHI	GRCGYC	2 cut(s)	71, 158
BsaI	GGTCTC	1 cut(s)	496
BsaJI	CCNNGG	3 cut(s)	207, 297, 1410
BsaWI	WCCGGW	3 cut(s)	440, 1632, 1863
BsaXI	ACNNNNNCTCC	2 cut(s)	6, 36
Bsc4I	CCNNNNNNNGG	7 cut(s)	514, 658, 1135, 1355, 1553, 1639, 1870
Bse1I	ACTGG	7 cut(s)	146, 280, 322, 346, 1026, 1292, 1395
Bse21I	CCTNAGG	1 cut(s)	513
Bse3DI	GCAATG	2 cut(s)	810, 1040
BseBI	CCWGG	2 cut(s)	426, 1608
BseDI	CCNNGG	3 cut(s)	207, 297, 1410
BseGI	GGATG	3 cut(s)	698, 1462, 1692
BseLI	CCNNNNNNNGG	7 cut(s)	514, 658, 1135, 1355, 1553, 1639, 1870
BseMI	GCAATG	2 cut(s)	810, 1040
BseMII	CTCAG	3 cut(s)	322, 776, 1494
BseNI	ACTGG	7 cut(s)	146, 280, 322, 346, 1026, 1292, 1395
BseRI	GAGGAG	1 cut(s)	14
BseXI	GCAGC	3 cut(s)	23, 659, 1703
Bsh1285I	CGRYCG	1 cut(s)	592
BshFI	GGCC	5 cut(s)	5, 125, 450, 1122, 1663
BshNI	GGYRCC	1 cut(s)	437
BsiEI	CGRYCG	1 cut(s)	592
BsiHKAI	GWGCWC	1 cut(s)	219
BsiHKCI	CYCGRG	1 cut(s)	1552
BsiSI	CCGG	6 cut(s)	441, 659, 1341, 1633, 1664, 1864
BsiWI	CGTACG	1 cut(s)	1103
BslFI	GGGAC	2 cut(s)	397, 579
BslI	CCNNNNNNNGG	7 cut(s)	514, 658, 1135, 1355, 1553, 1639, 1870
BsmAI	GTCTC	4 cut(s)	496, 1197, 1555, 1593
BsmFI	GGGAC	2 cut(s)	397, 579
BsnI	GGCC	5 cut(s)	5, 125, 450, 1122, 1663
Bso31I	GGTCTC	1 cut(s)	496
BsoBI	CYCGRG	1 cut(s)	1552
Bsp119I	TTCGAA	1 cut(s)	1428
Bsp1286I	GDGCHC	2 cut(s)	219, 1292
Bsp143I	GATC	7 cut(s)	198, 247, 311, 517, 589, 957, 1036
BspACI	CCGC	5 cut(s)	40, 375, 381, 407, 1119
BspANI	GGCC	5 cut(s)	5, 125, 450, 1122, 1663
BspCNI	CTCAG	3 cut(s)	321, 777, 1495
BspHI	TCATGA	1 cut(s)	1284
BspLI	GGNNCC	5 cut(s)	385, 386, 439, 1359, 1705
BspMAI	CTGCAG	1 cut(s)	744
BspPI	GGATC	2 cut(s)	525, 1031
BspQI	GCTCTTC	3 cut(s)	207, 255, 1632
BspT104I	TTCGAA	1 cut(s)	1428
BspT107I	GGYRCC	1 cut(s)	437
BspTNI	GGTCTC	1 cut(s)	496
BsrBI	CCGCTC	3 cut(s)	42, 381, 409
BsrDI	GCAATG	2 cut(s)	810, 1040
BsrI	ACTGG	7 cut(s)	146, 280, 322, 346, 1026, 1292, 1395
BssECI	CCNNGG	3 cut(s)	207, 297, 1410
BssMI	GATC	7 cut(s)	198, 247, 311, 517, 589, 957, 1036
BssNI	GRCGYC	2 cut(s)	71, 158
BssSI	CACGAG	1 cut(s)	493
BssT1I	CCWWGG	2 cut(s)	297, 1410
Bst2BI	CACGAG	1 cut(s)	493
Bst2UI	CCWGG	2 cut(s)	426, 1608
Bst4CI	ACNGT	7 cut(s)	528, 856, 1015, 1162, 1425, 1671, 1840
Bst6I	CTCTTC	5 cut(s)	207, 255, 1027, 1136, 1632
BstACI	GRCGYC	2 cut(s)	71, 158
BstAPI	GCANNNNNTGC	2 cut(s)	632, 748
BstBI	TTCGAA	1 cut(s)	1428
BstC8I	GCNNGC	8 cut(s)	105, 267, 271, 294, 500, 628, 1082, 1086
BstDEI	CTNAG	6 cut(s)	308, 513, 538, 767, 785, 1503
BstF5I	GGATG	3 cut(s)	698, 1462, 1692
BstH2I	RGCGCY	1 cut(s)	1542
BstHHI	GCGC	1 cut(s)	1541
BstKTI	GATC	7 cut(s)	201, 250, 314, 520, 592, 960, 1039
BstMAI	GTCTC	4 cut(s)	496, 1197, 1555, 1593
BstMBI	GATC	7 cut(s)	198, 247, 311, 517, 589, 957, 1036
BstMCI	CGRYCG	1 cut(s)	592
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 632, 692, 748, 809, 844, 1004
BstNI	CCWGG	2 cut(s)	426, 1608
BstNSI	RCATGY	2 cut(s)	630, 1387
BstSCI	CCNGG	4 cut(s)	424, 657, 1340, 1606
BstSFI	CTRYAG	2 cut(s)	740, 1350
BstV1I	GCAGC	3 cut(s)	23, 659, 1703
BstV2I	GAAGAC	4 cut(s)	66, 142, 296, 1119
BstX2I	RGATCY	1 cut(s)	1036
BstXI	CCANNNNNNTGG	1 cut(s)	121
BstYI	RGATCY	1 cut(s)	1036
Bsu36I	CCTNAGG	1 cut(s)	513
BsuI	GTATCC	1 cut(s)	1033
BsuRI	GGCC	5 cut(s)	5, 125, 450, 1122, 1663
BtsCI	GGATG	3 cut(s)	698, 1462, 1692
BtsI	GCAGTG	1 cut(s)	842
BtsIMutI	CAGTG	4 cut(s)	339, 842, 1033, 1299
Cac8I	GCNNGC	8 cut(s)	105, 267, 271, 294, 500, 628, 1082, 1086
CciI	TCATGA	1 cut(s)	1284
CfoI	GCGC	1 cut(s)	1541
Cfr13I	GGNCC	5 cut(s)	96, 111, 384, 1099, 1358
Csp6I	GTAC	6 cut(s)	325, 334, 789, 963, 1104, 1715
CviAII	CATG	9 cut(s)	62, 398, 575, 627, 748, 995, 1285, 1384, 1592
CviQI	GTAC	6 cut(s)	325, 334, 789, 963, 1104, 1715
DdeI	CTNAG	6 cut(s)	308, 513, 538, 767, 785, 1503
DpnI	GATC	7 cut(s)	200, 249, 313, 519, 591, 959, 1038
DpnII	GATC	7 cut(s)	198, 247, 311, 517, 589, 957, 1036
EaeI	YGGCCR	3 cut(s)	123, 1120, 1661
Eam1104I	CTCTTC	5 cut(s)	207, 255, 1027, 1136, 1632
EarI	CTCTTC	5 cut(s)	207, 255, 1027, 1136, 1632
Eco130I	CCWWGG	2 cut(s)	297, 1410
Eco24I	GRGCYC	1 cut(s)	1292
Eco31I	GGTCTC	1 cut(s)	496
Eco32I	GATATC	2 cut(s)	664, 919
Eco47I	GGWCC	5 cut(s)	96, 111, 384, 1099, 1358
Eco57I	CTGAAG	1 cut(s)	688
Eco81I	CCTNAGG	1 cut(s)	513
Eco88I	CYCGRG	1 cut(s)	1552
EcoO109I	RGGNCCY	2 cut(s)	384, 1358
EcoRII	CCWGG	2 cut(s)	424, 1606
EcoRV	GATATC	2 cut(s)	664, 919
EcoT14I	CCWWGG	2 cut(s)	297, 1410
EcoT38I	GRGCYC	1 cut(s)	1292
ErhI	CCWWGG	2 cut(s)	297, 1410
FaeI	CATG	9 cut(s)	65, 401, 578, 630, 751, 998, 1288, 1387, 1595
FaqI	GGGAC	2 cut(s)	397, 579
FatI	CATG	9 cut(s)	61, 397, 574, 626, 747, 994, 1284, 1383, 1591
FauI	CCCGC	3 cut(s)	368, 374, 414
FauNDI	CATATG	1 cut(s)	976
FbaI	TGATCA	2 cut(s)	247, 957
Fnu4HI	GCNGC	5 cut(s)	37, 40, 648, 1120, 1692
FokI	GGATG	3 cut(s)	685, 1449, 1699
FriOI	GRGCYC	1 cut(s)	1292
Fsp4HI	GCNGC	5 cut(s)	37, 40, 648, 1120, 1692
FspBI	CTAG	4 cut(s)	108, 813, 1050, 1695
GlaI	GCGC	1 cut(s)	1540
GluI	GCNGC	5 cut(s)	37, 40, 648, 1120, 1692
GsuI	CTGGAG	3 cut(s)	129, 248, 839
HaeII	RGCGCY	1 cut(s)	1542
HaeIII	GGCC	5 cut(s)	5, 125, 450, 1122, 1663
HapII	CCGG	6 cut(s)	441, 659, 1341, 1633, 1664, 1864
HhaI	GCGC	1 cut(s)	1541
Hin1I	GRCGYC	2 cut(s)	71, 158
Hin1II	CATG	9 cut(s)	65, 401, 578, 630, 751, 998, 1288, 1387, 1595
Hin6I	GCGC	1 cut(s)	1539
HinP1I	GCGC	1 cut(s)	1539
HincII	GTYRAC	3 cut(s)	162, 1276, 1509
HindII	GTYRAC	3 cut(s)	162, 1276, 1509
HindIII	AAGCTT	1 cut(s)	1820
HinfI	GANTC	7 cut(s)	182, 401, 614, 755, 1071, 1345, 1417
HpaI	GTTAAC	2 cut(s)	1276, 1509
HpaII	CCGG	6 cut(s)	441, 659, 1341, 1633, 1664, 1864
HphI	GGTGA	8 cut(s)	69, 254, 576, 705, 1224, 1426, 1622, 1760
Hpy166II	GTNNAC	4 cut(s)	162, 1276, 1499, 1509
Hpy188III	TCNNGA	9 cut(s)	154, 202, 227, 818, 943, 1055, 1168, 1285, 1554
Hpy8I	GTNNAC	4 cut(s)	162, 1276, 1499, 1509
Hpy99I	CGWCG	4 cut(s)	73, 160, 239, 1191
HpyAV	CCTTC	7 cut(s)	422, 712, 782, 817, 1055, 1245, 1630
HpyCH4III	ACNGT	7 cut(s)	528, 856, 1015, 1162, 1425, 1671, 1840
HpyCH4IV	ACGT	5 cut(s)	71, 158, 237, 1106, 1440
HpyCH4V	TGCA	5 cut(s)	502, 635, 742, 803, 1088
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 632, 692, 748, 809, 844, 1004
HpyF3I	CTNAG	6 cut(s)	308, 513, 538, 767, 785, 1503
HpySE526I	ACGT	5 cut(s)	71, 158, 237, 1106, 1440
Hsp92I	GRCGYC	2 cut(s)	71, 158
Hsp92II	CATG	9 cut(s)	65, 401, 578, 630, 751, 998, 1288, 1387, 1595
HspAI	GCGC	1 cut(s)	1539
KflI	GGGWCCC	1 cut(s)	384
Ksp22I	TGATCA	2 cut(s)	247, 957
KspAI	GTTAAC	2 cut(s)	1276, 1509
Kzo9I	GATC	7 cut(s)	198, 247, 311, 517, 589, 957, 1036
LguI	GCTCTTC	3 cut(s)	207, 255, 1632
LmnI	GCTCC	5 cut(s)	47, 378, 414, 623, 1703
Lsp1109I	GCAGC	3 cut(s)	23, 659, 1703
LweI	GCATC	5 cut(s)	263, 469, 568, 1132, 1287
MaeI	CTAG	4 cut(s)	108, 813, 1050, 1695
MaeII	ACGT	5 cut(s)	71, 158, 237, 1106, 1440
MaeIII	GTNAC	4 cut(s)	711, 1386, 1414, 1834
MalI	GATC	7 cut(s)	200, 249, 313, 519, 591, 959, 1038
MbiI	CCGCTC	3 cut(s)	42, 381, 409
MboI	GATC	7 cut(s)	198, 247, 311, 517, 589, 957, 1036
MflI	RGATCY	1 cut(s)	1036
MhlI	GDGCHC	2 cut(s)	219, 1292
MlsI	TGGCCA	1 cut(s)	125
MluCI	AATT	6 cut(s)	429, 561, 792, 982, 1676, 1850
MluNI	TGGCCA	1 cut(s)	125
MlyI	GAGTC	4 cut(s)	191, 749, 1065, 1411
MmeI	TCCRAC	7 cut(s)	33, 82, 417, 589, 811, 838, 1711
Mox20I	TGGCCA	1 cut(s)	125
MroXI	GAANNNNTTC	2 cut(s)	936, 1404
MscI	TGGCCA	1 cut(s)	125
MseI	TTAA	7 cut(s)	275, 654, 1245, 1275, 1508, 1806, 1879
MslI	CAYNNNNRTG	1 cut(s)	1283
Msp20I	TGGCCA	1 cut(s)	125
MspI	CCGG	6 cut(s)	441, 659, 1341, 1633, 1664, 1864
MspR9I	CCNGG	4 cut(s)	426, 659, 1342, 1608
MvaI	CCWGG	2 cut(s)	426, 1608
MwoI	GCNNNNNNNGC	7 cut(s)	11, 632, 692, 748, 809, 844, 1004
NciI	CCSGG	2 cut(s)	659, 1342
NdeI	CATATG	1 cut(s)	976
NdeII	GATC	7 cut(s)	198, 247, 311, 517, 589, 957, 1036
NlaIII	CATG	9 cut(s)	65, 401, 578, 630, 751, 998, 1288, 1387, 1595
NlaIV	GGNNCC	5 cut(s)	385, 386, 439, 1359, 1705
NmuCI	GTSAC	2 cut(s)	711, 1414
NspI	RCATGY	2 cut(s)	630, 1387
NspV	TTCGAA	1 cut(s)	1428
PaeI	GCATGC	1 cut(s)	630
PagI	TCATGA	1 cut(s)	1284
PciI	ACATGT	1 cut(s)	1383
PciSI	GCTCTTC	3 cut(s)	207, 255, 1632
PdmI	GAANNNNTTC	2 cut(s)	936, 1404
PfeI	GAWTC	3 cut(s)	401, 614, 1345
Pfl23II	CGTACG	1 cut(s)	1103
PkrI	GCNGC	5 cut(s)	38, 41, 649, 1121, 1693
Ple19I	CGATCG	1 cut(s)	592
PleI	GAGTC	4 cut(s)	190, 749, 1065, 1411
PpsI	GAGTC	4 cut(s)	190, 749, 1065, 1411
PpuMI	RGGWCCY	2 cut(s)	384, 1358
PscI	ACATGT	1 cut(s)	1383
Psp5II	RGGWCCY	2 cut(s)	384, 1358
Psp6I	CCWGG	2 cut(s)	424, 1606
PspGI	CCWGG	2 cut(s)	424, 1606
PspLI	CGTACG	1 cut(s)	1103
PspN4I	GGNNCC	5 cut(s)	385, 386, 439, 1359, 1705
PspPI	GGNCC	5 cut(s)	96, 111, 384, 1099, 1358
PspPPI	RGGWCCY	2 cut(s)	384, 1358
PstI	CTGCAG	1 cut(s)	744
PsuI	RGATCY	1 cut(s)	1036
PvuI	CGATCG	1 cut(s)	592
RsaI	GTAC	6 cut(s)	326, 335, 790, 964, 1105, 1716
RsaNI	GTAC	6 cut(s)	325, 334, 789, 963, 1104, 1715
RseI	CAYNNNNRTG	1 cut(s)	1283
SapI	GCTCTTC	3 cut(s)	207, 255, 1632
SaqAI	TTAA	7 cut(s)	275, 654, 1245, 1275, 1508, 1806, 1879
SatI	GCNGC	5 cut(s)	37, 40, 648, 1120, 1692
Sau3AI	GATC	7 cut(s)	198, 247, 311, 517, 589, 957, 1036
Sau96I	GGNCC	5 cut(s)	96, 111, 384, 1099, 1358
SchI	GAGTC	4 cut(s)	191, 749, 1065, 1411
ScrFI	CCNGG	4 cut(s)	426, 659, 1342, 1608
SduI	GDGCHC	2 cut(s)	219, 1292
SfaNI	GCATC	5 cut(s)	263, 469, 568, 1132, 1287
SfcI	CTRYAG	2 cut(s)	740, 1350
SfuI	TTCGAA	1 cut(s)	1428
SinI	GGWCC	5 cut(s)	96, 111, 384, 1099, 1358
SmiMI	CAYNNNNRTG	1 cut(s)	1283
SmlI	CTYRAG	1 cut(s)	671
SmoI	CTYRAG	1 cut(s)	671
SphI	GCATGC	1 cut(s)	630
Sse9I	AATT	6 cut(s)	429, 561, 792, 982, 1676, 1850
SsiI	CCGC	5 cut(s)	40, 375, 381, 407, 1119
SspI	AATATT	1 cut(s)	1811
SspMI	CTAG	4 cut(s)	108, 813, 1050, 1695
StyD4I	CCNGG	4 cut(s)	424, 657, 1340, 1606
StyI	CCWWGG	2 cut(s)	297, 1410
TaaI	ACNGT	7 cut(s)	528, 856, 1015, 1162, 1425, 1671, 1840
TaiI	ACGT	5 cut(s)	74, 161, 240, 1109, 1443
TaqI	TCGA	3 cut(s)	520, 666, 1428
TaqII	GACCGA	1 cut(s)	221
TasI	AATT	6 cut(s)	429, 561, 792, 982, 1676, 1850
TatI	WGTACW	2 cut(s)	788, 962
TauI	GCSGC	2 cut(s)	42, 1122
TfiI	GAWTC	3 cut(s)	401, 614, 1345
Tru1I	TTAA	7 cut(s)	275, 654, 1245, 1275, 1508, 1806, 1879
Tru9I	TTAA	7 cut(s)	275, 654, 1245, 1275, 1508, 1806, 1879
TscAI	CASTG	4 cut(s)	346, 849, 1033, 1299
TseFI	GTSAC	2 cut(s)	711, 1414
TseI	GCWGC	3 cut(s)	36, 647, 1691
Tsp45I	GTSAC	2 cut(s)	711, 1414
TspDTI	ATGAA	2 cut(s)	78, 736
TspGWI	ACGGA	2 cut(s)	1091, 1332
TspRI	CASTG	4 cut(s)	346, 849, 1033, 1299
VpaK11BI	GGWCC	5 cut(s)	96, 111, 384, 1099, 1358
XapI	RAATTY	1 cut(s)	1676
XceI	RCATGY	2 cut(s)	630, 1387
XmnI	GAANNNNTTC	2 cut(s)	936, 1404
XspI	CTAG	4 cut(s)	108, 813, 1050, 1695
ZraI	GACGTC	2 cut(s)	72, 159

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.