Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0000740

Physical Location & Seq

Genomic Coordinates Reverse (-)

77508318 .. 77512326

Gene Structure 4009 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG493700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1368 bp

ATGCCTCACTCACCCAATCTTAGCTTTCTTCATATACTCAGTTCAATGACTGGTGCAATGATGGCTAATTTGGAGAAAGAAGTTGAAAGGCTATCGCCAGATCTTGTTGCACCATCAATAGCTTACCTCTCTGTTATTGCTGACATCTTACCCATAGAAACGCTTCTGTGGAAGCTTCAAATGATTAATTCAGCTTCATCGTTTGCCAATTCACGCCTCCATGCTGTAAAAGCTCAAACCTTGATACTTTCCAGTGGAAGGGATCCATTGCTACCTAGTCAAGAGGAAGGTCTAATAAGACTCCAACAGCTGCTTGCAAATTGTGAGATCTGTAGGTTTGAAGACTGCGGCCATTTCCTCTTCTTGGAAGATGCTTTTGATTTGGTAACTGTCATCAAGCAGGTTGGTGTCTATCGTCGTTCAAAAGAACGAGATTTTGTTTTAGATTACATACCACCATCCCCTTCCGAATTAAGAACTTTACGGGACAATAACAGATGGATTAACATCATGAGCCCAGTGATGCTTTCAACTTTAGCAGATGAGACGATAGTGAGGGGCTTTGCTGGGATTCCATCAGAGGGTCCTGTATTGTATGTCGGTTATCACATGTTACTGGGATCTGATATCGCTCCATTGGTAGCACAGTTGTGGGAACAAAAAGGCATCCATCTGCGAGGGATTGCACATCCAATGATGTTTATGAAATCGAAACAAGGAAATTTAGTTGAGTTACCCTTTTCCGACTATTTCAGACTGATGGGTGCTGTTCCTGTCTCGGGAAAAAACTTTTTCAGGCTACTCTCTACAAAGTCTCATATCCTACTGTATCCTGGTGGAATTCGTGAGGCTCTTCACAGAAAGGGGGAAGCATACAAGTTATTCTGGCCAGAACGGTCAGAGTTTGTGAGGATGGCAGCTAGATTTGGAGCGAAAATCGTACCCTTTGGTACAGTTGGAGAAGATGATTTTTTTGACATATTACTCGATTATGAAGACCAGATGAAAATTCCTTTCCTCAAGAAATATATAGATGCATTATCGGATGAAGTTGGAAAGCTGAGGACTACCGTTGATGGGGAGGTAGGAAACCAAGATGTATACTTCCCAGGAATTCTACCAAAATCTCCCGGCCGGTTTTATTATTATTTTGGGAAAACAATTGAAACAGACGGGAGGAAGCAGGAATTACGAGACAGGGAGAAAGCTCAAGAGTTGTATTTACAAGTGAAATCAGAGGTTGAGAACTGCATTGCTTATTTGCAGGAAAAAAGAGAGAGTGATCCTTACAGAAGCCTTGTGTCTCGGCTACTCTTCCAGGCCAGACATGGCTTTACATCTGAAGTTCCTGCATTTGACCTCGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010287 GO:0016101 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0019432 GO:0033306 GO:0034308 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:1901576 GO:1901615 GO:1903173

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6 DAGAT Hydrolase_4

Protein Analysis

455

Amino Acids

51.76

Weight (kDa)

6.0

Isoelectric Point (pI)

50.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGAT	PF03982	248 - 333	1.5e-09	Diacylglycerol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000740)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02030 AT3G02030 AT3G26820 AT3G26820 AT3G26840 AT5G41120 AT5G41120 AT5G41120 AT5G41130 AT5G41130 AT5G41130
fragaria_vesca	FvH4_3g35400 FvH4_3g39220 FvH4_3g39220 FvH4_3g39270 FvH4_3g39280 FvH4_3g39280 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060
malus_domestica	MD09G1005800.v1.1 MD17G1009900.v1.1
prunus_persica	Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1
pyrus_communis	pycom111g00420
rosa_chinensis	RchiOBHm_Chr2g0176241 RchiOBHm_Chr5g0070671 RchiOBHm_Chr5g0070681 RchiOBHm_Chr5g0070691
rosa_laevigata	RLG00000022394 RLG00000036149 RLG00000036150 RLG00000036152
rosa_multiflora	Rmu_co8466433.1_g000001 Rmu_sc0000249.1_g000001 Rmu_sc0000405.1_g000016 Rmu_sc0005197.1_g000009 Rmu_sc0005197.1_g000015 Rmu_sc0036323.1_g000001
rosa_roxburghii	Rroxscaffold_1G00010480 Rroxscaffold_1G00010530 Rroxscaffold_1G00010550 Rroxscaffold_1G00010560 Rroxscaffold_2G00076650 Rroxscaffold_5G00357250
rosa_rugosa	Rorug02G0592200 Rorug02G0592300 Rorug05G0407500 Rorug05G0407800
rosa_samantha	Rh2AG670700 Rh2BG682200 Rh2CG645000 Rh2DG695800 Rh5AG463200 Rh5AG463300 Rh5AG463400 Rh5BG481200 Rh5CG505400 Rh5CG505500 Rh5DG493300 Rh5DG493600 Rh5DG493700
rosa_wichuraiana	Rw2G055020 Rw5G043110 Rw5G043120 Rw5G043130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	391
AccI	GTMKAC	1 cut(s)	1101
AciI	CCGC	1 cut(s)	348
AclWI	GGATC	4 cut(s)	257, 270, 628, 1277
AcoI	YGGCCR	3 cut(s)	349, 887, 1132
AcsI	RAATTY	4 cut(s)	721, 840, 1008, 1113
AcuI	CTGAAG	1 cut(s)	1362
AfaI	GTAC	2 cut(s)	942, 952
AfiI	CCNNNNNNNGG	5 cut(s)	258, 364, 581, 779, 1077
AflIII	ACRYGT	1 cut(s)	609
AgsI	TTSAA	7 cut(s)	45, 86, 179, 341, 423, 531, 1166
AjnI	CCWGG	3 cut(s)	832, 1108, 1317
AleI	CACNNNNGTG	1 cut(s)	649
AluBI	AGCT	9 cut(s)	24, 122, 175, 194, 233, 310, 920, 1060, 1208
AluI	AGCT	9 cut(s)	24, 122, 175, 194, 233, 310, 920, 1060, 1208
Alw26I	GTCTC	5 cut(s)	539, 781, 819, 1188, 1308
AlwI	GGATC	4 cut(s)	257, 270, 628, 1277
Ama87I	CYCGRG	1 cut(s)	778
AoxI	GGCC	4 cut(s)	349, 887, 1132, 1320
ApeKI	GCWGC	2 cut(s)	310, 917
ApoI	RAATTY	4 cut(s)	721, 840, 1008, 1113
AseI	ATTAAT	1 cut(s)	186
Asp700I	GAANNNNTTC	1 cut(s)	162
AspS9I	GGNCC	1 cut(s)	584
AsuC2I	CCSGG	1 cut(s)	1131
AsuHPI	GGTGA	1 cut(s)	3
AvaI	CYCGRG	1 cut(s)	778
AvaII	GGWCC	1 cut(s)	584
BalI	TGGCCA	1 cut(s)	889
BamHI	GGATCC	1 cut(s)	262
BanII	GRGCYC	1 cut(s)	518
BbsI	GAAGAC	2 cut(s)	348, 1002
BbvCI	CCTCAGC	1 cut(s)	1061
BbvI	GCAGC	2 cut(s)	297, 929
BccI	CCATC	9 cut(s)	55, 121, 466, 492, 583, 678, 754, 907, 1070
BciT130I	CCWGG	3 cut(s)	834, 1110, 1319
BciVI	GTATCC	1 cut(s)	840
BcnI	CCSGG	1 cut(s)	1131
BcoDI	GTCTC	5 cut(s)	539, 781, 819, 1188, 1308
BfaI	CTAG	2 cut(s)	276, 921
BfmI	CTRYAG	1 cut(s)	331
BfuAI	ACCTGC	1 cut(s)	391
BfuI	GTATCC	1 cut(s)	840
BglII	AGATCT	2 cut(s)	100, 327
BisI	GCNGC	3 cut(s)	311, 349, 918
BlsI	GCNGC	3 cut(s)	312, 350, 919
Bme1390I	CCNGG	4 cut(s)	834, 1110, 1131, 1319
Bme18I	GGWCC	1 cut(s)	584
BmeT110I	CYCGRG	1 cut(s)	778
BmgT120I	GGNCC	1 cut(s)	584
BmiI	GGNNCC	2 cut(s)	264, 585
BmrFI	CCNGG	4 cut(s)	834, 1110, 1131, 1319
BmrI	ACTGGG	2 cut(s)	512, 626
BmsI	GCATC	4 cut(s)	361, 513, 675, 1024
BmuI	ACTGGG	2 cut(s)	512, 626
BpiI	GAAGAC	2 cut(s)	348, 1002
Bpu10I	CCTNAGC	1 cut(s)	1061
BpuEI	CTTGAG	2 cut(s)	1004, 1194
BpuMI	CCSGG	1 cut(s)	1131
BsaBI	GATNNNNATC	1 cut(s)	506
BsaJI	CCNNGG	1 cut(s)	1108
Bsc4I	CCNNNNNNNGG	5 cut(s)	258, 364, 581, 779, 1077
Bse118I	RCCGGY	1 cut(s)	1134
Bse1I	ACTGG	4 cut(s)	55, 252, 518, 621
Bse3DI	GCAATG	3 cut(s)	63, 266, 1251
Bse8I	GATNNNNATC	1 cut(s)	506
BseBI	CCWGG	3 cut(s)	834, 1110, 1319
BseDI	CCNNGG	1 cut(s)	1108
BseGI	GGATG	5 cut(s)	458, 666, 688, 918, 1051
BseJI	GATNNNNATC	1 cut(s)	506
BseLI	CCNNNNNNNGG	5 cut(s)	258, 364, 581, 779, 1077
BseMI	GCAATG	3 cut(s)	63, 266, 1251
BseMII	CTCAG	2 cut(s)	52, 1052
BseNI	ACTGG	4 cut(s)	55, 252, 518, 621
BseX3I	CGGCCG	1 cut(s)	1132
BseXI	GCAGC	2 cut(s)	297, 929
BseYI	CCCAGC	1 cut(s)	566
Bsh1285I	CGRYCG	1 cut(s)	1135
BshFI	GGCC	4 cut(s)	351, 889, 1134, 1322
BsiEI	CGRYCG	1 cut(s)	1135
BsiHKCI	CYCGRG	1 cut(s)	778
BsiSI	CCGG	2 cut(s)	1131, 1135
BslFI	GGGAC	1 cut(s)	500
BslI	CCNNNNNNNGG	5 cut(s)	258, 364, 581, 779, 1077
BsmAI	GTCTC	5 cut(s)	539, 781, 819, 1188, 1308
BsmBI	CGTCTC	1 cut(s)	539
BsmFI	GGGAC	1 cut(s)	500
BsnI	GGCC	4 cut(s)	351, 889, 1134, 1322
BsoBI	CYCGRG	1 cut(s)	778
Bsp1286I	GDGCHC	1 cut(s)	518
Bsp143I	GATC	5 cut(s)	100, 262, 327, 620, 1282
BspACI	CCGC	1 cut(s)	348
BspANI	GGCC	4 cut(s)	351, 889, 1134, 1322
BspCNI	CTCAG	2 cut(s)	51, 1053
BspHI	TCATGA	1 cut(s)	510
BspLI	GGNNCC	2 cut(s)	264, 585
BspMI	ACCTGC	1 cut(s)	391
BspPI	GGATC	4 cut(s)	257, 270, 628, 1277
BspQI	GCTCTTC	1 cut(s)	858
BsrDI	GCAATG	3 cut(s)	63, 266, 1251
BsrFI	RCCGGY	1 cut(s)	1134
BsrI	ACTGG	4 cut(s)	55, 252, 518, 621
BssAI	RCCGGY	1 cut(s)	1134
BssECI	CCNNGG	1 cut(s)	1108
BssMI	GATC	5 cut(s)	100, 262, 327, 620, 1282
BssNAI	GTATAC	1 cut(s)	1102
Bst1107I	GTATAC	1 cut(s)	1102
Bst2UI	CCWGG	3 cut(s)	834, 1110, 1319
Bst4CI	ACNGT	6 cut(s)	391, 648, 828, 897, 955, 1072
Bst6I	CTCTTC	3 cut(s)	365, 858, 1319
BstC8I	GCNNGC	1 cut(s)	315
BstDEI	CTNAG	3 cut(s)	20, 38, 1061
BstF5I	GGATG	5 cut(s)	458, 666, 688, 918, 1051
BstKTI	GATC	5 cut(s)	103, 265, 330, 623, 1285
BstMAI	GTCTC	5 cut(s)	539, 781, 819, 1188, 1308
BstMBI	GATC	5 cut(s)	100, 262, 327, 620, 1282
BstMCI	CGRYCG	1 cut(s)	1135
BstMWI	GCNNNNNNNGC	2 cut(s)	62, 230
BstNI	CCWGG	3 cut(s)	834, 1110, 1319
BstNSI	RCATGY	1 cut(s)	613
BstSCI	CCNGG	4 cut(s)	832, 1108, 1129, 1317
BstSFI	CTRYAG	1 cut(s)	331
BstV1I	GCAGC	2 cut(s)	297, 929
BstV2I	GAAGAC	2 cut(s)	348, 1002
BstX2I	RGATCY	4 cut(s)	100, 262, 327, 620
BstYI	RGATCY	4 cut(s)	100, 262, 327, 620
BstZ17I	GTATAC	1 cut(s)	1102
BstZI	CGGCCG	1 cut(s)	1132
BsuI	GTATCC	1 cut(s)	840
BsuRI	GGCC	4 cut(s)	351, 889, 1134, 1322
BtsCI	GGATG	5 cut(s)	458, 666, 688, 918, 1051
BtsIMutI	CAGTG	2 cut(s)	259, 525
BveI	ACCTGC	1 cut(s)	391
Cac8I	GCNNGC	1 cut(s)	315
CciI	TCATGA	1 cut(s)	510
Cfr10I	RCCGGY	1 cut(s)	1134
Cfr13I	GGNCC	1 cut(s)	584
Csp6I	GTAC	2 cut(s)	941, 951
CviAII	CATG	4 cut(s)	221, 511, 610, 1328
CviQI	GTAC	2 cut(s)	941, 951
DdeI	CTNAG	3 cut(s)	20, 38, 1061
DpnI	GATC	5 cut(s)	102, 264, 329, 622, 1284
DpnII	GATC	5 cut(s)	100, 262, 327, 620, 1282
EaeI	YGGCCR	3 cut(s)	349, 887, 1132
EagI	CGGCCG	1 cut(s)	1132
Eam1104I	CTCTTC	3 cut(s)	365, 858, 1319
EarI	CTCTTC	3 cut(s)	365, 858, 1319
EclXI	CGGCCG	1 cut(s)	1132
Eco24I	GRGCYC	1 cut(s)	518
Eco32I	GATATC	1 cut(s)	628
Eco47I	GGWCC	1 cut(s)	584
Eco52I	CGGCCG	1 cut(s)	1132
Eco57I	CTGAAG	1 cut(s)	1362
Eco88I	CYCGRG	1 cut(s)	778
EcoO109I	RGGNCCY	1 cut(s)	584
EcoRI	GAATTC	2 cut(s)	840, 1113
EcoRII	CCWGG	3 cut(s)	832, 1108, 1317
EcoRV	GATATC	1 cut(s)	628
EcoT22I	ATGCAT	1 cut(s)	1039
EcoT38I	GRGCYC	1 cut(s)	518
Esp3I	CGTCTC	1 cut(s)	539
FaeI	CATG	4 cut(s)	224, 514, 613, 1331
FaqI	GGGAC	1 cut(s)	500
FatI	CATG	4 cut(s)	220, 510, 609, 1327
FblI	GTMKAC	1 cut(s)	1101
Fnu4HI	GCNGC	3 cut(s)	311, 349, 918
FokI	GGATG	5 cut(s)	445, 653, 675, 925, 1058
FriOI	GRGCYC	1 cut(s)	518
Fsp4HI	GCNGC	3 cut(s)	311, 349, 918
FspBI	CTAG	2 cut(s)	276, 921
GluI	GCNGC	3 cut(s)	311, 349, 918
GsaI	CCCAGC	1 cut(s)	570
HaeIII	GGCC	4 cut(s)	351, 889, 1134, 1322
HapII	CCGG	2 cut(s)	1131, 1135
Hin1II	CATG	4 cut(s)	224, 514, 613, 1331
HindIII	AAGCTT	1 cut(s)	173
HinfI	GANTC	2 cut(s)	300, 571
HpaII	CCGG	2 cut(s)	1131, 1135
HphI	GGTGA	1 cut(s)	3
Hpy166II	GTNNAC	1 cut(s)	1102
Hpy188I	TCNGA	9 cut(s)	469, 580, 625, 745, 755, 901, 1045, 1237, 1342
Hpy188III	TCNNGA	6 cut(s)	281, 511, 780, 845, 1021, 1211
Hpy8I	GTNNAC	1 cut(s)	1102
Hpy99I	CGWCG	1 cut(s)	420
HpyAV	CCTTC	3 cut(s)	252, 281, 474
HpyCH4III	ACNGT	6 cut(s)	391, 648, 828, 897, 955, 1072
HpyCH4V	TGCA	8 cut(s)	56, 110, 317, 686, 1037, 1251, 1264, 1352
HpyF10VI	GCNNNNNNNGC	2 cut(s)	62, 230
HpyF3I	CTNAG	3 cut(s)	20, 38, 1061
Hsp92II	CATG	4 cut(s)	224, 514, 613, 1331
Kzo9I	GATC	5 cut(s)	100, 262, 327, 620, 1282
LguI	GCTCTTC	1 cut(s)	858
LmnI	GCTCC	2 cut(s)	637, 929
Lsp1109I	GCAGC	2 cut(s)	297, 929
LweI	GCATC	4 cut(s)	361, 513, 675, 1024
MaeI	CTAG	2 cut(s)	276, 921
MaeIII	GTNAC	3 cut(s)	385, 612, 732
MalI	GATC	5 cut(s)	102, 264, 329, 622, 1284
MboI	GATC	5 cut(s)	100, 262, 327, 620, 1282
MboII	GAAGA	8 cut(s)	20, 352, 353, 380, 845, 974, 1007, 1306
MfeI	CAATTG	1 cut(s)	1161
MflI	RGATCY	4 cut(s)	100, 262, 327, 620
MhlI	GDGCHC	1 cut(s)	518
MlsI	TGGCCA	1 cut(s)	889
MluNI	TGGCCA	1 cut(s)	889
MlyI	GAGTC	1 cut(s)	294
MmeI	TCCRAC	4 cut(s)	328, 768, 937, 1033
Mox20I	TGGCCA	1 cut(s)	889
Mph1103I	ATGCAT	1 cut(s)	1039
MroXI	GAANNNNTTC	1 cut(s)	162
MscI	TGGCCA	1 cut(s)	889
MseI	TTAA	4 cut(s)	186, 473, 504, 1366
MslI	CAYNNNNRTG	1 cut(s)	649
Msp20I	TGGCCA	1 cut(s)	889
MspA1I	CMGCKG	1 cut(s)	310
MspI	CCGG	2 cut(s)	1131, 1135
MspR9I	CCNGG	4 cut(s)	834, 1110, 1131, 1319
MunI	CAATTG	1 cut(s)	1161
MvaI	CCWGG	3 cut(s)	834, 1110, 1319
MwoI	GCNNNNNNNGC	2 cut(s)	62, 230
NciI	CCSGG	1 cut(s)	1131
NdeII	GATC	5 cut(s)	100, 262, 327, 620, 1282
NlaIII	CATG	4 cut(s)	224, 514, 613, 1331
NlaIV	GGNNCC	2 cut(s)	264, 585
NmeAIII	GCCGAG	1 cut(s)	1285
NsiI	ATGCAT	1 cut(s)	1039
NspI	RCATGY	1 cut(s)	613
OliI	CACNNNNGTG	1 cut(s)	649
PagI	TCATGA	1 cut(s)	510
PciI	ACATGT	1 cut(s)	609
PciSI	GCTCTTC	1 cut(s)	858
PdmI	GAANNNNTTC	1 cut(s)	162
PfeI	GAWTC	1 cut(s)	571
PkrI	GCNGC	3 cut(s)	312, 350, 919
PleI	GAGTC	1 cut(s)	294
PpsI	GAGTC	1 cut(s)	294
PpuMI	RGGWCCY	1 cut(s)	584
PscI	ACATGT	1 cut(s)	609
PshBI	ATTAAT	1 cut(s)	186
Psp5II	RGGWCCY	1 cut(s)	584
Psp6I	CCWGG	3 cut(s)	832, 1108, 1317
PspFI	CCCAGC	1 cut(s)	566
PspGI	CCWGG	3 cut(s)	832, 1108, 1317
PspN4I	GGNNCC	2 cut(s)	264, 585
PspPI	GGNCC	1 cut(s)	584
PspPPI	RGGWCCY	1 cut(s)	584
PsuI	RGATCY	4 cut(s)	100, 262, 327, 620
PvuII	CAGCTG	1 cut(s)	310
RsaI	GTAC	2 cut(s)	942, 952
RsaNI	GTAC	2 cut(s)	941, 951
RseI	CAYNNNNRTG	1 cut(s)	649
SapI	GCTCTTC	1 cut(s)	858
SaqAI	TTAA	4 cut(s)	186, 473, 504, 1366
SatI	GCNGC	3 cut(s)	311, 349, 918
Sau3AI	GATC	5 cut(s)	100, 262, 327, 620, 1282
Sau96I	GGNCC	1 cut(s)	584
SchI	GAGTC	1 cut(s)	294
ScrFI	CCNGG	4 cut(s)	834, 1110, 1131, 1319
SduI	GDGCHC	1 cut(s)	518
SfaNI	GCATC	4 cut(s)	361, 513, 675, 1024
SfcI	CTRYAG	1 cut(s)	331
SinI	GGWCC	1 cut(s)	584
SmiMI	CAYNNNNRTG	1 cut(s)	649
SmlI	CTYRAG	2 cut(s)	1019, 1209
SmoI	CTYRAG	2 cut(s)	1019, 1209
SsiI	CCGC	1 cut(s)	348
SspMI	CTAG	2 cut(s)	276, 921
StyD4I	CCNGG	4 cut(s)	832, 1108, 1129, 1317
TaaI	ACNGT	6 cut(s)	391, 648, 828, 897, 955, 1072
TaqI	TCGA	3 cut(s)	710, 987, 1362
TauI	GCSGC	1 cut(s)	351
TfiI	GAWTC	1 cut(s)	571
Tru1I	TTAA	4 cut(s)	186, 473, 504, 1366
Tru9I	TTAA	4 cut(s)	186, 473, 504, 1366
TscAI	CASTG	2 cut(s)	259, 525
TseI	GCWGC	2 cut(s)	310, 917
TspDTI	ATGAA	6 cut(s)	20, 186, 719, 1008, 1019, 1062
TspRI	CASTG	2 cut(s)	259, 525
VpaK11BI	GGWCC	1 cut(s)	584
VspI	ATTAAT	1 cut(s)	186
XapI	RAATTY	4 cut(s)	721, 840, 1008, 1113
XceI	RCATGY	1 cut(s)	613
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1325
XmiI	GTMKAC	1 cut(s)	1101
XmnI	GAANNNNTTC	1 cut(s)	162
XspI	CTAG	2 cut(s)	276, 921
Zsp2I	ATGCAT	1 cut(s)	1039

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.