Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000740

Physical Location & Seq

Genomic Coordinates Forward (+)

612314 .. 617814

Gene Structure 5501 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1009900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGGCCTCCACCGGAGTTTGTCTTTTCCCCGCCCGCCTGCCATCCGTTATCCGTCAGGAACTGACGTCGTCATCATCATCCGGAAAATCTAAGTTCAATCCGATCTCGACTCCATTTCGGAGGCTAGCCGCTGTGTCTACTGACCACACTCCGACGAGCCTGACCACAGTGAATATCGGAAACGGCAAGGCCTCTGAGAAGGATGCTCATACGACGACGACAAAGTCAGCTGCAGAGACGAAGAGGCAGGCTGGTCAGGAGGAGAAGAAGTTGGATGCGGAAGAGCAGGGCCTGAGCTTGAAGGATTACTTCGAGCAAGCCAAGGATTTCATCAGCTCCGACGGTGGCGGTGGTGGTGGACCGCCTCGCTGGTTCTCTCCTTTGGGCTGCGGTTCCCGCTTGGACAATTCTCCTCTGCTTCTTTTTTTGCCTGGAATTGATGGGACTGGACTTGGGCTCACAAGGCATCATCAGAAACTTGGAAAGATTTTTGATGTCTGGTGCTTGCATATTCCTGTAAAGGATCGAACACCATTCACAGACCTAGTGAAGCTGGTTGAAAGAACAGTTCGATCCGAGCATCGTCTCGCTCCAAATAGGCCTATTTATCTTGTTGGAGAATCTCTTGGAGCATGTCTTGCGCTTTCTGTGGCTGCCCTTAACCCTGATATTGACCTTGTTCTAATTTTGGCTAATCCAGCTACGTCCTTCAGCAAGTCCCAACTTCAACCATTAATACCTTTACTGCAGGTCATTCCTGACTCACTTCCTCTTAGCCTTCCTCACATAGTGAGCTCAACGACAGGTTTTCTGATGGGTAACTTGGTGACGGGACTTAGACGTCCTCTGCCACAGACAGTTGAGAAGCTATCACGTGATCTTCTTGCGTCAACGTCTTACCTCTCTGCTCTTGGTGACATCTTACCTCGAGAAACACTTCTGTGGAAGCTACAAATGATTAGATCAGCATCTCAATATGCCAATTCACGTCTCCACGCTGTGAAAGCTGAAACGCTGATACTTTCCAGTGGACTGGATCAGTTGTTACCTAGTCAAGCGGAAGGAGAAAGACTCAAACGCACCCTTGCAAAATGTGAGATTCGTACGTTTGCCAACAGAGGTCATTTCCTCTTCCTGGAAGATAATTTTGATCTGGTAACTGTCATCAAGGCCACTTGTTTCTATCGTCGTTCCAGTACTCATGACTTTGTTACTGATTACATACCACTAACGCCTTCAGAAGTCAAAAATATAATTGAATCAAACAGATTTTTAACCATGGCCACTAGCCCAGTCATGTTCTCAACTTTAGCAGATGGGAAGATTGTGAGGGGCCTTGCCGGGATTCCTTCCGAGGGCCCTGTATTGTTTGTTGGTTATCACATGTTACTGGGGTTTGAAATGGCTCCCTTGGTAATTCAGATGTTTGAGGAAAGAAACATCCAATTGCGAGGAATAGCACATCCGATGATGTTTATGAAGTTCAGAGAAGGAAAGTTTCCTGATCCCTCTATTATATTCGACACAATCAGACTTCTGGGTGCAGTTCCTGTCTCTGGTGCAAACTTATACAAACTCCTCTCCACCAAGTCTCACGTCCTACTATATCCAGGTGGAGTGCGTGAGGCTCTTCACCGGAAGGGGGAGGAATATAAGTTATTCTGGCCACAACAGTCGGAGTTTGTGAGAATGGCAGCTAGATTTGGAGCCAAAATCGTGCCTTTTGGCGCTGTTGGAGAAGATGATATTGGTGAACTTTTATTGGATTACAACGACCAAATGAGAATTCCTCCACTCAAGAAGTCTATACAGGAGGTAACAGATGAGGTTGCGAAAGTGAGAATTGGCGTTGGCGGGGAGGTAGGAAACCAGGATGTACACCTGCCAGGAATCATACCGAAATTTCCAGGCAGGTTCTATTATTATTTTGGAAAACCAATTGAAACAGAAGGGCGGAAGCAGGAATTACGAGACAGGGAGAAAGCTCATGAACTGTATTTACAAGTGAAATCAGAAGTTGAGGATTGCCTTGCTTATTTGAAGGAGAAAAGAGAGAAGGATCCTTACAGAACGTTATTGTCTCGGATACAATACCAGGTCAGACATGGCTTCACATCTGAAATTCCTACATTCGATATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010287 GO:0016101 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0019432 GO:0033306 GO:0034308 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:1901576 GO:1901615 GO:1903173

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6 DAGAT Hydrolase_4

Protein Analysis

714

Amino Acids

79.25

Weight (kDa)

8.87

Isoelectric Point (pI)

45.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_6	PF12697	174 - 384	2.6e-07	Alpha/beta hydrolase family
Hydrolase_4	PF12146	180 - 381	2.5e-09	Serine aminopeptidase, S33
DAGAT	PF03982	509 - 587	4.4e-08	Diacylglycerol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000740)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02030 AT3G02030 AT3G26820 AT3G26820 AT3G26840 AT5G41120 AT5G41120 AT5G41120 AT5G41130 AT5G41130 AT5G41130
fragaria_vesca	FvH4_3g35400 FvH4_3g39220 FvH4_3g39220 FvH4_3g39270 FvH4_3g39280 FvH4_3g39280 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060
malus_domestica	MD09G1005800.v1.1 MD17G1009900.v1.1
prunus_persica	Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1
pyrus_communis	pycom111g00420
rosa_chinensis	RchiOBHm_Chr2g0176241 RchiOBHm_Chr5g0070671 RchiOBHm_Chr5g0070681 RchiOBHm_Chr5g0070691
rosa_laevigata	RLG00000022394 RLG00000036149 RLG00000036150 RLG00000036152
rosa_multiflora	Rmu_co8466433.1_g000001 Rmu_sc0000249.1_g000001 Rmu_sc0000405.1_g000016 Rmu_sc0005197.1_g000009 Rmu_sc0005197.1_g000015 Rmu_sc0036323.1_g000001
rosa_roxburghii	Rroxscaffold_1G00010480 Rroxscaffold_1G00010530 Rroxscaffold_1G00010550 Rroxscaffold_1G00010560 Rroxscaffold_2G00076650 Rroxscaffold_5G00357250
rosa_rugosa	Rorug02G0592200 Rorug02G0592300 Rorug05G0407500 Rorug05G0407800
rosa_samantha	Rh2AG670700 Rh2BG682200 Rh2CG645000 Rh2DG695800 Rh5AG463200 Rh5AG463300 Rh5AG463400 Rh5BG481200 Rh5CG505400 Rh5CG505500 Rh5DG493300 Rh5DG493600 Rh5DG493700
rosa_wichuraiana	Rw2G055020 Rw5G043110 Rw5G043120 Rw5G043130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1890
AasI	GACNNNNNNGTC	2 cut(s)	223, 840
AatII	GACGTC	2 cut(s)	68, 844
Acc36I	ACCTGC	3 cut(s)	739, 1890, 1902
AccI	GTMKAC	1 cut(s)	137
AccIII	TCCGGA	1 cut(s)	80
AclI	AACGTT	1 cut(s)	2072
AclWI	GGATC	6 cut(s)	531, 567, 1044, 1499, 2054, 2067
AcoI	YGGCCR	2 cut(s)	1281, 1664
AcsI	RAATTY	3 cut(s)	1785, 1901, 2121
AcuI	CTGAAG	2 cut(s)	694, 1221
AcvI	CACGTG	1 cut(s)	875
AcyI	GRCGYC	2 cut(s)	65, 841
AdeI	CACNNNGTG	2 cut(s)	790, 1000
AfaI	GTAC	3 cut(s)	1105, 1198, 1878
AfiI	CCNNNNNNNGG	4 cut(s)	1135, 1355, 1556, 1642
AflIII	ACRYGT	1 cut(s)	1383
AgsI	TTSAA	8 cut(s)	97, 301, 560, 728, 1259, 1400, 1943, 2041
AjiI	CACGTC	2 cut(s)	989, 1597
AjnI	CCWGG	7 cut(s)	430, 1134, 1609, 1869, 1885, 1906, 2094
AjuI	GAANNNNNNNTTGG	4 cut(s)	1731, 1763, 1770, 1802
AleI	CACNNNNGTG	1 cut(s)	940
Alw21I	GWGCWC	1 cut(s)	797
Alw26I	GTCTC	7 cut(s)	230, 590, 995, 1558, 1596, 1965, 2085
AlwI	GGATC	6 cut(s)	531, 567, 1044, 1499, 2054, 2067
AlwNI	CAGNNNCTG	3 cut(s)	61, 292, 1550
Ama87I	CYCGRG	1 cut(s)	927
Aor13HI	TCCGGA	1 cut(s)	80
AoxI	GGCC	9 cut(s)	3, 189, 289, 599, 1170, 1281, 1333, 1357, 1664
ApaI	GGGCCC	1 cut(s)	1361
ApeKI	GCWGC	4 cut(s)	230, 387, 653, 1694
ApoI	RAATTY	3 cut(s)	1785, 1901, 2121
AseI	ATTAAT	1 cut(s)	734
Asp700I	GAANNNNTTC	1 cut(s)	936
AspLEI	GCGC	2 cut(s)	643, 1730
AspS9I	GGNCC	5 cut(s)	289, 359, 1333, 1357, 1358
AsuC2I	CCSGG	1 cut(s)	1342
AsuHPI	GGTGA	4 cut(s)	838, 926, 1625, 1763
AsuNHI	GCTAGC	1 cut(s)	124
AvaI	CYCGRG	1 cut(s)	927
AvaII	GGWCC	1 cut(s)	359
BaeGI	GKGCMC	1 cut(s)	1361
BalI	TGGCCA	2 cut(s)	1283, 1666
BamHI	GGATCC	1 cut(s)	2059
BanII	GRGCYC	3 cut(s)	459, 797, 1361
BarI	GAAGNNNNNNTAC	2 cut(s)	2048, 2080
BbrPI	CACGTG	1 cut(s)	875
Bbv12I	GWGCWC	1 cut(s)	797
BbvI	GCAGC	4 cut(s)	217, 374, 640, 1706
BccI	CCATC	4 cut(s)	49, 434, 808, 1310
BceAI	ACGGC	1 cut(s)	199
BciT130I	CCWGG	7 cut(s)	432, 1136, 1611, 1871, 1887, 1908, 2096
BciVI	GTATCC	1 cut(s)	2079
BcnI	CCSGG	1 cut(s)	1342
BcoDI	GTCTC	7 cut(s)	230, 590, 995, 1558, 1596, 1965, 2085
BfaI	CTAG	5 cut(s)	125, 545, 1050, 1287, 1698
BfmI	CTRYAG	2 cut(s)	231, 746
BfoI	RGCGCY	1 cut(s)	1731
BfuAI	ACCTGC	3 cut(s)	739, 1890, 1902
BfuI	GTATCC	1 cut(s)	2079
BisI	GCNGC	5 cut(s)	129, 231, 388, 654, 1695
BlsI	GCNGC	5 cut(s)	130, 232, 389, 655, 1696
BmcAI	AGTACT	1 cut(s)	1198
Bme1390I	CCNGG	8 cut(s)	432, 1136, 1342, 1611, 1871, 1887, 1908, 2096
Bme18I	GGWCC	1 cut(s)	359
BmeT110I	CYCGRG	1 cut(s)	927
BmgBI	CACGTC	2 cut(s)	989, 1597
BmgT120I	GGNCC	5 cut(s)	289, 359, 1333, 1357, 1358
BmiI	GGNNCC	6 cut(s)	394, 1334, 1359, 1407, 1708, 2061
BmrFI	CCNGG	8 cut(s)	432, 1136, 1342, 1611, 1871, 1887, 1908, 2096
BmrI	ACTGGG	2 cut(s)	1286, 1400
BmsI	GCATC	5 cut(s)	193, 265, 475, 589, 977
BmtI	GCTAGC	1 cut(s)	128
BmuI	ACTGGG	2 cut(s)	1286, 1400
BplI	GAGNNNNNCTC	4 cut(s)	1056, 1088, 1774, 1806
Bpu10I	CCTNAGC	1 cut(s)	293
BpuEI	CTTGAG	1 cut(s)	1781
BpuMI	CCSGG	1 cut(s)	1342
BsaAI	YACGTR	1 cut(s)	875
BsaBI	GATNNNNATC	1 cut(s)	967
BsaHI	GRCGYC	2 cut(s)	65, 841
BsaJI	CCNNGG	4 cut(s)	321, 1278, 1353, 1410
BsaWI	WCCGGW	3 cut(s)	11, 80, 1635
BsaXI	ACNNNNNCTCC	2 cut(s)	254, 284
Bsc4I	CCNNNNNNNGG	4 cut(s)	1135, 1355, 1556, 1642
Bse1I	ACTGG	6 cut(s)	451, 1026, 1038, 1194, 1292, 1395
Bse8I	GATNNNNATC	1 cut(s)	967
BseAI	TCCGGA	1 cut(s)	80
BseBI	CCWGG	7 cut(s)	432, 1136, 1611, 1871, 1887, 1908, 2096
BseDI	CCNNGG	4 cut(s)	321, 1278, 1353, 1410
BseGI	GGATG	7 cut(s)	41, 77, 208, 280, 1440, 1462, 1879
BseJI	GATNNNNATC	1 cut(s)	967
BseLI	CCNNNNNNNGG	4 cut(s)	1135, 1355, 1556, 1642
BseMII	CTCAG	2 cut(s)	186, 284
BseNI	ACTGG	6 cut(s)	451, 1026, 1038, 1194, 1292, 1395
BseRI	GAGGAG	3 cut(s)	275, 402, 1568
BseSI	GKGCMC	1 cut(s)	1361
BseXI	GCAGC	4 cut(s)	217, 374, 640, 1706
BsgI	GTGCAG	1 cut(s)	1563
BshFI	GGCC	9 cut(s)	5, 191, 291, 601, 1172, 1283, 1335, 1359, 1666
BsiHKAI	GWGCWC	1 cut(s)	797
BsiHKCI	CYCGRG	1 cut(s)	927
BsiSI	CCGG	4 cut(s)	12, 81, 1341, 1636
BsiWI	CGTACG	1 cut(s)	1103
BslFI	GGGAC	3 cut(s)	457, 703, 846
BslI	CCNNNNNNNGG	4 cut(s)	1135, 1355, 1556, 1642
BsmAI	GTCTC	7 cut(s)	230, 590, 995, 1558, 1596, 1965, 2085
BsmBI	CGTCTC	3 cut(s)	230, 590, 995
BsmFI	GGGAC	3 cut(s)	457, 703, 846
BsnI	GGCC	9 cut(s)	5, 191, 291, 601, 1172, 1283, 1335, 1359, 1666
BsoBI	CYCGRG	1 cut(s)	927
Bsp120I	GGGCCC	1 cut(s)	1357
Bsp1286I	GDGCHC	3 cut(s)	459, 797, 1361
Bsp13I	TCCGGA	1 cut(s)	80
Bsp1407I	TGTACA	1 cut(s)	1876
Bsp143I	GATC	9 cut(s)	102, 523, 572, 877, 962, 1036, 1150, 1504, 2059
Bsp19I	CCATGG	1 cut(s)	1278
BspANI	GGCC	9 cut(s)	5, 191, 291, 601, 1172, 1283, 1335, 1359, 1666
BspCNI	CTCAG	2 cut(s)	187, 285
BspEI	TCCGGA	1 cut(s)	80
BspHI	TCATGA	2 cut(s)	1201, 1987
BspLI	GGNNCC	6 cut(s)	394, 1334, 1359, 1407, 1708, 2061
BspMAI	CTGCAG	2 cut(s)	235, 750
BspMI	ACCTGC	3 cut(s)	739, 1890, 1902
BspOI	GCTAGC	1 cut(s)	128
BspPI	GGATC	6 cut(s)	531, 567, 1044, 1499, 2054, 2067
BspQI	GCTCTTC	2 cut(s)	276, 1635
BsrGI	TGTACA	1 cut(s)	1876
BsrI	ACTGG	6 cut(s)	451, 1026, 1038, 1194, 1292, 1395
BssECI	CCNNGG	4 cut(s)	321, 1278, 1353, 1410
BssMI	GATC	9 cut(s)	102, 523, 572, 877, 962, 1036, 1150, 1504, 2059
BssNI	GRCGYC	2 cut(s)	65, 841
BssT1I	CCWWGG	3 cut(s)	321, 1278, 1410
Bst2UI	CCWGG	7 cut(s)	432, 1136, 1611, 1871, 1887, 1908, 2096
Bst4CI	ACNGT	7 cut(s)	169, 344, 568, 859, 1162, 1674, 1995
Bst6I	CTCTTC	4 cut(s)	236, 276, 1136, 1635
BstACI	GRCGYC	2 cut(s)	65, 841
BstAPI	GCANNNNNTGC	1 cut(s)	638
BstAUI	TGTACA	1 cut(s)	1876
BstBAI	YACGTR	1 cut(s)	875
BstC8I	GCNNGC	6 cut(s)	34, 38, 126, 249, 318, 506
BstDEI	CTNAG	5 cut(s)	90, 195, 293, 773, 836
BstDSI	CCRYGG	1 cut(s)	1278
BstF5I	GGATG	7 cut(s)	41, 77, 208, 280, 1440, 1462, 1879
BstH2I	RGCGCY	1 cut(s)	1731
BstHHI	GCGC	2 cut(s)	643, 1730
BstKTI	GATC	9 cut(s)	105, 526, 575, 880, 965, 1039, 1153, 1507, 2062
BstMAI	GTCTC	7 cut(s)	230, 590, 995, 1558, 1596, 1965, 2085
BstMBI	GATC	9 cut(s)	102, 523, 572, 877, 962, 1036, 1150, 1504, 2059
BstMWI	GCNNNNNNNGC	5 cut(s)	396, 463, 638, 698, 1004
BstNI	CCWGG	7 cut(s)	432, 1136, 1611, 1871, 1887, 1908, 2096
BstNSI	RCATGY	2 cut(s)	636, 1387
BstSCI	CCNGG	8 cut(s)	430, 1134, 1340, 1609, 1869, 1885, 1906, 2094
BstSFI	CTRYAG	2 cut(s)	231, 746
BstSLI	GKGCMC	1 cut(s)	1361
BstV1I	GCAGC	4 cut(s)	217, 374, 640, 1706
BstX2I	RGATCY	1 cut(s)	2059
BstXI	CCANNNNNNTGG	1 cut(s)	1033
BstYI	RGATCY	1 cut(s)	2059
BsuI	GTATCC	1 cut(s)	2079
BsuRI	GGCC	9 cut(s)	5, 191, 291, 601, 1172, 1283, 1335, 1359, 1666
BtgI	CCRYGG	1 cut(s)	1278
BtrI	CACGTC	2 cut(s)	989, 1597
BtsCI	GGATG	7 cut(s)	41, 77, 208, 280, 1440, 1462, 1879
BtsIMutI	CAGTG	2 cut(s)	174, 1033
BveI	ACCTGC	3 cut(s)	739, 1890, 1902
Cac8I	GCNNGC	6 cut(s)	34, 38, 126, 249, 318, 506
CaiI	CAGNNNCTG	3 cut(s)	61, 292, 1550
CciI	TCATGA	2 cut(s)	1201, 1987
CfoI	GCGC	2 cut(s)	643, 1730
Cfr13I	GGNCC	5 cut(s)	289, 359, 1333, 1357, 1358
CseI	GACGC	1 cut(s)	876
CsiI	ACCWGGT	1 cut(s)	2094
Csp6I	GTAC	3 cut(s)	1104, 1197, 1877
CviAII	CATG	7 cut(s)	633, 1202, 1279, 1297, 1384, 1988, 2105
CviQI	GTAC	3 cut(s)	1104, 1197, 1877
DdeI	CTNAG	5 cut(s)	90, 195, 293, 773, 836
DpnI	GATC	9 cut(s)	104, 525, 574, 879, 964, 1038, 1152, 1506, 2061
DpnII	GATC	9 cut(s)	102, 523, 572, 877, 962, 1036, 1150, 1504, 2059
DraIII	CACNNNGTG	2 cut(s)	790, 1000
DrdI	GACNNNNNNGTC	2 cut(s)	223, 840
DseDI	GACNNNNNNGTC	2 cut(s)	223, 840
EaeI	YGGCCR	2 cut(s)	1281, 1664
Eam1104I	CTCTTC	4 cut(s)	236, 276, 1136, 1635
EarI	CTCTTC	4 cut(s)	236, 276, 1136, 1635
EciI	GGCGGA	1 cut(s)	1969
Ecl136II	GAGCTC	1 cut(s)	795
Eco130I	CCWWGG	3 cut(s)	321, 1278, 1410
Eco147I	AGGCCT	2 cut(s)	191, 601
Eco24I	GRGCYC	3 cut(s)	459, 797, 1361
Eco32I	GATATC	1 cut(s)	2137
Eco47I	GGWCC	1 cut(s)	359
Eco53kI	GAGCTC	1 cut(s)	795
Eco57I	CTGAAG	2 cut(s)	694, 1221
Eco72I	CACGTG	1 cut(s)	875
Eco88I	CYCGRG	1 cut(s)	927
EcoICRI	GAGCTC	1 cut(s)	795
EcoO109I	RGGNCCY	4 cut(s)	289, 1333, 1357, 1358
EcoRI	GAATTC	1 cut(s)	1785
EcoRII	CCWGG	7 cut(s)	430, 1134, 1609, 1869, 1885, 1906, 2094
EcoRV	GATATC	1 cut(s)	2137
EcoT14I	CCWWGG	3 cut(s)	321, 1278, 1410
EcoT38I	GRGCYC	3 cut(s)	459, 797, 1361
ErhI	CCWWGG	3 cut(s)	321, 1278, 1410
Esp3I	CGTCTC	3 cut(s)	230, 590, 995
FaeI	CATG	7 cut(s)	636, 1205, 1282, 1300, 1387, 1991, 2108
FalI	AAGNNNNNCTT	2 cut(s)	293, 325
FaqI	GGGAC	3 cut(s)	457, 703, 846
FatI	CATG	7 cut(s)	632, 1201, 1278, 1296, 1383, 1987, 2104
FauI	CCCGC	4 cut(s)	37, 41, 404, 1847
FblI	GTMKAC	1 cut(s)	137
Fnu4HI	GCNGC	5 cut(s)	129, 231, 388, 654, 1695
FokI	GGATG	7 cut(s)	28, 64, 215, 287, 1427, 1449, 1886
FriOI	GRGCYC	3 cut(s)	459, 797, 1361
Fsp4HI	GCNGC	5 cut(s)	129, 231, 388, 654, 1695
FspBI	CTAG	5 cut(s)	125, 545, 1050, 1287, 1698
GlaI	GCGC	2 cut(s)	642, 1729
GluI	GCNGC	5 cut(s)	129, 231, 388, 654, 1695
HaeII	RGCGCY	1 cut(s)	1731
HaeIII	GGCC	9 cut(s)	5, 191, 291, 601, 1172, 1283, 1335, 1359, 1666
HapII	CCGG	4 cut(s)	12, 81, 1341, 1636
HgaI	GACGC	1 cut(s)	876
HhaI	GCGC	2 cut(s)	643, 1730
Hin1I	GRCGYC	2 cut(s)	65, 841
Hin1II	CATG	7 cut(s)	636, 1205, 1282, 1300, 1387, 1991, 2108
Hin6I	GCGC	2 cut(s)	641, 1728
HinP1I	GCGC	2 cut(s)	641, 1728
HincII	GTYRAC	1 cut(s)	891
HindII	GTYRAC	1 cut(s)	891
HinfI	GANTC	8 cut(s)	109, 620, 761, 1071, 1099, 1259, 1345, 1890
HpaII	CCGG	4 cut(s)	12, 81, 1341, 1636
HphI	GGTGA	4 cut(s)	838, 926, 1625, 1763
Hpy166II	GTNNAC	6 cut(s)	138, 359, 891, 1031, 1754, 1879
Hpy8I	GTNNAC	6 cut(s)	138, 359, 891, 1031, 1754, 1879
Hpy99I	CGWCG	6 cut(s)	70, 157, 217, 220, 344, 1191
HpyCH4III	ACNGT	7 cut(s)	169, 344, 568, 859, 1162, 1674, 1995
HpyCH4IV	ACGT	9 cut(s)	65, 704, 841, 874, 893, 988, 1106, 1596, 2072
HpyCH4V	TGCA	6 cut(s)	233, 508, 748, 1088, 1544, 1562
HpyF10VI	GCNNNNNNNGC	5 cut(s)	396, 463, 638, 698, 1004
HpyF3I	CTNAG	5 cut(s)	90, 195, 293, 773, 836
HpySE526I	ACGT	9 cut(s)	65, 704, 841, 874, 893, 988, 1106, 1596, 2072
Hsp92I	GRCGYC	2 cut(s)	65, 841
Hsp92II	CATG	7 cut(s)	636, 1205, 1282, 1300, 1387, 1991, 2108
HspAI	GCGC	2 cut(s)	641, 1728
Kpn2I	TCCGGA	1 cut(s)	80
Kzo9I	GATC	9 cut(s)	102, 523, 572, 877, 962, 1036, 1150, 1504, 2059
LguI	GCTCTTC	2 cut(s)	276, 1635
LmnI	GCTCC	5 cut(s)	341, 595, 629, 1411, 1706
Lsp1109I	GCAGC	4 cut(s)	217, 374, 640, 1706
LweI	GCATC	5 cut(s)	193, 265, 475, 589, 977
MabI	ACCWGGT	1 cut(s)	2094
MaeI	CTAG	5 cut(s)	125, 545, 1050, 1287, 1698
MaeII	ACGT	9 cut(s)	65, 704, 841, 874, 893, 988, 1106, 1596, 2072
MaeIII	GTNAC	8 cut(s)	818, 826, 914, 1044, 1156, 1210, 1386, 1816
MalI	GATC	9 cut(s)	104, 525, 574, 879, 964, 1038, 1152, 1506, 2061
MboI	GATC	9 cut(s)	102, 523, 572, 877, 962, 1036, 1150, 1504, 2059
MboII	GAAGA	9 cut(s)	253, 277, 293, 872, 1123, 1151, 1333, 1622, 1751
MfeI	CAATTG	2 cut(s)	1445, 1938
MflI	RGATCY	1 cut(s)	2059
MhlI	GDGCHC	3 cut(s)	459, 797, 1361
MlsI	TGGCCA	2 cut(s)	1283, 1666
MluNI	TGGCCA	2 cut(s)	1283, 1666
MlyI	GAGTC	3 cut(s)	103, 755, 1065
MmeI	TCCRAC	6 cut(s)	176, 252, 363, 595, 1656, 1714
Mox20I	TGGCCA	2 cut(s)	1283, 1666
MroI	TCCGGA	1 cut(s)	80
MroXI	GAANNNNTTC	1 cut(s)	936
MscI	TGGCCA	2 cut(s)	1283, 1666
MseI	TTAA	3 cut(s)	660, 734, 1274
MslI	CAYNNNNRTG	1 cut(s)	940
Msp20I	TGGCCA	2 cut(s)	1283, 1666
MspA1I	CMGCKG	2 cut(s)	131, 230
MspI	CCGG	4 cut(s)	12, 81, 1341, 1636
MspR9I	CCNGG	8 cut(s)	432, 1136, 1342, 1611, 1871, 1887, 1908, 2096
MunI	CAATTG	2 cut(s)	1445, 1938
MvaI	CCWGG	7 cut(s)	432, 1136, 1611, 1871, 1887, 1908, 2096
MwoI	GCNNNNNNNGC	5 cut(s)	396, 463, 638, 698, 1004
NciI	CCSGG	1 cut(s)	1342
NcoI	CCATGG	1 cut(s)	1278
NdeII	GATC	9 cut(s)	102, 523, 572, 877, 962, 1036, 1150, 1504, 2059
NheI	GCTAGC	1 cut(s)	124
NlaIII	CATG	7 cut(s)	636, 1205, 1282, 1300, 1387, 1991, 2108
NlaIV	GGNNCC	6 cut(s)	394, 1334, 1359, 1407, 1708, 2061
NmuCI	GTSAC	2 cut(s)	826, 914
NspI	RCATGY	2 cut(s)	636, 1387
OliI	CACNNNNGTG	1 cut(s)	940
PaeR7I	CTCGAG	1 cut(s)	927
PagI	TCATGA	2 cut(s)	1201, 1987
PaqCI	CACCTGC	1 cut(s)	1890
PceI	AGGCCT	2 cut(s)	191, 601
PciI	ACATGT	1 cut(s)	1383
PciSI	GCTCTTC	2 cut(s)	276, 1635
PdmI	GAANNNNTTC	1 cut(s)	936
PfeI	GAWTC	5 cut(s)	620, 1099, 1259, 1345, 1890
Pfl23II	CGTACG	1 cut(s)	1103
PflFI	GACNNNGTC	2 cut(s)	67, 223
PfoI	TCCNGGA	1 cut(s)	1134
PkrI	GCNGC	5 cut(s)	130, 232, 389, 655, 1696
PleI	GAGTC	3 cut(s)	103, 755, 1065
PmaCI	CACGTG	1 cut(s)	875
PmlI	CACGTG	1 cut(s)	875
PpsI	GAGTC	3 cut(s)	103, 755, 1065
Ppu21I	YACGTR	1 cut(s)	875
PscI	ACATGT	1 cut(s)	1383
PshBI	ATTAAT	1 cut(s)	734
Psp124BI	GAGCTC	1 cut(s)	797
Psp1406I	AACGTT	1 cut(s)	2072
Psp6I	CCWGG	7 cut(s)	430, 1134, 1609, 1869, 1885, 1906, 2094
PspCI	CACGTG	1 cut(s)	875
PspGI	CCWGG	7 cut(s)	430, 1134, 1609, 1869, 1885, 1906, 2094
PspLI	CGTACG	1 cut(s)	1103
PspN4I	GGNNCC	6 cut(s)	394, 1334, 1359, 1407, 1708, 2061
PspOMI	GGGCCC	1 cut(s)	1357
PspPI	GGNCC	5 cut(s)	289, 359, 1333, 1357, 1358
PsrI	GAACNNNNNNTAC	2 cut(s)	1983, 2015
PstI	CTGCAG	2 cut(s)	235, 750
PstNI	CAGNNNCTG	3 cut(s)	61, 292, 1550
PsuI	RGATCY	1 cut(s)	2059
PsyI	GACNNNGTC	2 cut(s)	67, 223
PvuII	CAGCTG	1 cut(s)	230
RsaI	GTAC	3 cut(s)	1105, 1198, 1878
RsaNI	GTAC	3 cut(s)	1104, 1197, 1877
RseI	CAYNNNNRTG	1 cut(s)	940
SacI	GAGCTC	1 cut(s)	797
SapI	GCTCTTC	2 cut(s)	276, 1635
SaqAI	TTAA	3 cut(s)	660, 734, 1274
SatI	GCNGC	5 cut(s)	129, 231, 388, 654, 1695
Sau3AI	GATC	9 cut(s)	102, 523, 572, 877, 962, 1036, 1150, 1504, 2059
Sau96I	GGNCC	5 cut(s)	289, 359, 1333, 1357, 1358
ScaI	AGTACT	1 cut(s)	1198
SchI	GAGTC	3 cut(s)	103, 755, 1065
ScrFI	CCNGG	8 cut(s)	432, 1136, 1342, 1611, 1871, 1887, 1908, 2096
SduI	GDGCHC	3 cut(s)	459, 797, 1361
SexAI	ACCWGGT	1 cut(s)	2094
SfaNI	GCATC	5 cut(s)	193, 265, 475, 589, 977
SfcI	CTRYAG	2 cut(s)	231, 746
Sfr274I	CTCGAG	1 cut(s)	927
SinI	GGWCC	1 cut(s)	359
SlaI	CTCGAG	1 cut(s)	927
SmiMI	CAYNNNNRTG	1 cut(s)	940
SmlI	CTYRAG	2 cut(s)	927, 1796
SmoI	CTYRAG	2 cut(s)	927, 1796
SseBI	AGGCCT	2 cut(s)	191, 601
SspMI	CTAG	5 cut(s)	125, 545, 1050, 1287, 1698
SstI	GAGCTC	1 cut(s)	797
StuI	AGGCCT	2 cut(s)	191, 601
StyD4I	CCNGG	8 cut(s)	430, 1134, 1340, 1609, 1869, 1885, 1906, 2094
StyI	CCWWGG	3 cut(s)	321, 1278, 1410
TaaI	ACNGT	7 cut(s)	169, 344, 568, 859, 1162, 1674, 1995
TaiI	ACGT	9 cut(s)	68, 707, 844, 877, 896, 991, 1109, 1599, 2075
TaqI	TCGA	7 cut(s)	107, 312, 526, 571, 928, 1521, 2133
TatI	WGTACW	2 cut(s)	1196, 1876
TauI	GCSGC	1 cut(s)	131
TfiI	GAWTC	5 cut(s)	620, 1099, 1259, 1345, 1890
Tru1I	TTAA	3 cut(s)	660, 734, 1274
Tru9I	TTAA	3 cut(s)	660, 734, 1274
TscAI	CASTG	2 cut(s)	174, 1033
TseFI	GTSAC	2 cut(s)	826, 914
TseI	GCWGC	4 cut(s)	230, 387, 653, 1694
Tsp45I	GTSAC	2 cut(s)	826, 914
TspDTI	ATGAA	3 cut(s)	319, 1493, 2004
TspGWI	ACGGA	2 cut(s)	34, 41
TspRI	CASTG	2 cut(s)	174, 1033
Tth111I	GACNNNGTC	2 cut(s)	67, 223
VpaK11BI	GGWCC	1 cut(s)	359
VspI	ATTAAT	1 cut(s)	734
XapI	RAATTY	3 cut(s)	1785, 1901, 2121
XceI	RCATGY	2 cut(s)	636, 1387
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2102
XhoI	CTCGAG	1 cut(s)	927
XmiI	GTMKAC	1 cut(s)	137
XmnI	GAANNNNTTC	1 cut(s)	936
XspI	CTAG	5 cut(s)	125, 545, 1050, 1287, 1698
ZraI	GACGTC	2 cut(s)	66, 842
ZrmI	AGTACT	1 cut(s)	1198

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.