Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000740

Physical Location & Seq

Genomic Coordinates Forward (+)

432008 .. 437619

Gene Structure 5612 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1005800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2136 bp

ATGGCCTCCGCCGGAGTTTGTCCTTTCCCCGCCCGCCTGCCATCCGTTATCCGTCAGGAACTGACGTCGTCGTCATCATCCTCATCCGGAAGATCGAAGTTCAATCCGATCTCGATTCCGTCGCGGAGGCTAGCCGCTGCGTCAACAGACCACACTCCGACGATTCTGACAGCAGCGAACATCGGAAGCGGCAGGTCGTCTGAGAAGGAAGCTCATACGACGACGACGACGACGTCAGCTGCAGAGACCAAGAGGCAGGCTCGTCAGGAGGAGCACAGGTTCGATGCGGAAGAGCAAGGCCTGAGCTTGAAGGATTACTTCGAGCAAGCTAAGGATTTCATCAGCTCCGACGGTGGACCGCCTCGCTGGTTCTCTCCTTTGGGCGGCGGGTCCCGCTTGGCCGATTCTCCTCTGCTTCTCTTTTTGCCTGGAATTGATGGCACTGGACTTGGGCTGATAAGGCATCATCAGAAACTTGGAAAGATTTTCGATGTCTGGTGCTTGCATATCCCTGCCAAGGATCGAACAACATTCACAGACCTAGTGAAGCTGGTTGAAAGAACAGTTCGGTCACAGCATCGTCTCGCACCAAATAGGCCTATATATCTTGTTGGAGAATCTCTTGGAGCATGCCTTGCGCTTTCTGTGGCTGCCCTTAACCCTGATATTGACCTTGTTCTAATTTTGGCTAATCCAGCTACGTCCTTCAGCAAGTCCCAGCTTCAACCATTAATACCTTTACTGCAGGTCATTCCTGACTCATTTCCTCTCAGCCTTCCTCACATACTGAGCTCAATGACAGGTTTTCTGATGGGTAACTTGGAGACGGGACTTGGACTTCCTCTGCCACAGACAGTTGAGAAGCTATCACGCGATCTTCTTGCATCAGCATCTTACCTTTCTGTTCTTGCTGATATCTTACCCAGAGAAACACTTCTGTGGAAGCTACAAATGATTAGATCAGCATCTCCATATGCCAATTCACGTCTCCACGCTGTAAAAGCTCAAACGCTGATACTTTCCAGTGGACAGGATCAGTTGTTACCTAGTCAAGCGGAAGGAGAAAGACTGAAACACACCCTTGCAAAATGTGAGATTCGTATGTTTGCTAACAGCGGCCATTTCCTCTTCTTGGAAGATCCTTTTGATTTGGTAACTGTCATCAAGGCAACTGGTGTCTATCGTCGTTCCAGTACCCATGACTTTGTTACTGATTACATACCGCCAACACCTTCAGAAGTCAAAAATATAATTGAATCAAACAGATTGTTAACCACGGTCACTAGCCCAGTGATGTTCTCAACTTTACCAGATGGGAAGATTGTGAGGGGCCTTGCCGGGATTCCTTCTGAGGGTCCTGTATTGTTTGTTGGTTATCACATGTTACTGGGGTTTGAATTGGCTCCCATGGTAACTCAGATGATGGAGGAAAGAAACATCCTAATGCGAGGAATAGCACATCCGATGATGTTTATAAAATTGAGAGAAGGAAAGTTTCCTGATCTATCAGCATTCGACACATTCAGACTTATGGGTGCAGTTCCTGTCTCTGGTGCAAACTTTTACAAACTTCTCTCTACAAAGTCTCACGTCCTACTCTATCCAGGTGGAGTGCGTGAGGCTCTTCACCGGAAGGGGGAGGAATATAAGTTATTCTGGCCAGAACAGTCGGAGTTTGTGAGAATGGCAGCTAGATTTGGAGCCAAAATTGTACCTTTTGGCGCCGTTGGAGAAGATGATATTGGTGAACTATTTTTGGATTATGACGACCAAATGAGAATTCCTCCGCTTAAGGACTTTATACAGGAGTTAACAGATGAGGTTGCGAAAGTGAGAAAGGGCGTTGGTGGGGAGGTAGGAAACCAGGATGTACACCTACCAGGAATCATACCGAAAATTCCTGGCAGGTTCTATTATTATTTTGGAAAACCAATTGAAACAGAAGGGAGGAAGCAGGAATTACGAGACAGGGAGAAAGCCCATGAACTGTATTTACAAGTGAAGTCGGAAGTTGAGAATTGCCTTGCTTATTTGAGGGAGAAAAGAGAGAAGGATCCGTATAGAACGTTATTGTCTAGGATACAATACCAGGCCTTGTCTGGCCTCACATCTGAAATTCCGACATTCGATATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010287 GO:0016101 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0019432 GO:0033306 GO:0034308 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:1901576 GO:1901615 GO:1903173

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6 DAGAT Hydrolase_4

Protein Analysis

712

Amino Acids

78.82

Weight (kDa)

7.71

Isoelectric Point (pI)

47.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_6	PF12697	172 - 385	3.5e-09	Alpha/beta hydrolase family
Hydrolase_4	PF12146	179 - 379	3.5e-10	Serine aminopeptidase, S33
DAGAT	PF03982	497 - 584	5.2e-08	Diacylglycerol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000740)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02030 AT3G02030 AT3G26820 AT3G26820 AT3G26840 AT5G41120 AT5G41120 AT5G41120 AT5G41130 AT5G41130 AT5G41130
fragaria_vesca	FvH4_3g35400 FvH4_3g39220 FvH4_3g39220 FvH4_3g39270 FvH4_3g39280 FvH4_3g39280 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060
malus_domestica	MD09G1005800.v1.1 MD17G1009900.v1.1
prunus_persica	Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1
pyrus_communis	pycom111g00420
rosa_chinensis	RchiOBHm_Chr2g0176241 RchiOBHm_Chr5g0070671 RchiOBHm_Chr5g0070681 RchiOBHm_Chr5g0070691
rosa_laevigata	RLG00000022394 RLG00000036149 RLG00000036150 RLG00000036152
rosa_multiflora	Rmu_co8466433.1_g000001 Rmu_sc0000249.1_g000001 Rmu_sc0000405.1_g000016 Rmu_sc0005197.1_g000009 Rmu_sc0005197.1_g000015 Rmu_sc0036323.1_g000001
rosa_roxburghii	Rroxscaffold_1G00010480 Rroxscaffold_1G00010530 Rroxscaffold_1G00010550 Rroxscaffold_1G00010560 Rroxscaffold_2G00076650 Rroxscaffold_5G00357250
rosa_rugosa	Rorug02G0592200 Rorug02G0592300 Rorug05G0407500 Rorug05G0407800
rosa_samantha	Rh2AG670700 Rh2BG682200 Rh2CG645000 Rh2DG695800 Rh5AG463200 Rh5AG463300 Rh5AG463400 Rh5BG481200 Rh5CG505400 Rh5CG505500 Rh5DG493300 Rh5DG493600 Rh5DG493700
rosa_wichuraiana	Rw2G055020 Rw5G043110 Rw5G043120 Rw5G043130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1475
AasI	GACNNNNNNGTC	2 cut(s)	70, 232
AatII	GACGTC	2 cut(s)	68, 236
Acc36I	ACCTGC	3 cut(s)	183, 736, 1896
AccB1I	GGYRCC	1 cut(s)	1721
AccII	CGCG	2 cut(s)	124, 873
AccIII	TCCGGA	1 cut(s)	86
AclI	AACGTT	1 cut(s)	2066
AclWI	GGATC	5 cut(s)	528, 1041, 1133, 2048, 2061
AcoI	YGGCCR	3 cut(s)	399, 1117, 1658
AcsI	RAATTY	3 cut(s)	1779, 1896, 2115
AcuI	CTGAAG	2 cut(s)	691, 1218
AcyI	GRCGYC	3 cut(s)	65, 233, 1722
AfaI	GTAC	3 cut(s)	1195, 1713, 1872
AfiI	CCNNNNNNNGG	6 cut(s)	383, 517, 1132, 1352, 1550, 1636
AflII	CTTAAG	1 cut(s)	1790
AflIII	ACRYGT	1 cut(s)	1380
AgsI	TTSAA	7 cut(s)	103, 310, 557, 725, 1256, 1397, 1937
AjiI	CACGTC	2 cut(s)	986, 1591
AjnI	CCWGG	6 cut(s)	427, 1603, 1863, 1879, 1900, 2088
AjuI	GAANNNNNNNTTGG	4 cut(s)	1725, 1757, 1764, 1796
AleI	CACNNNNGTG	1 cut(s)	937
AloI	GAACNNNNNNTCC	2 cut(s)	263, 295
Alw21I	GWGCWC	2 cut(s)	276, 794
Alw26I	GTCTC	7 cut(s)	239, 587, 818, 992, 1552, 1590, 1959
AlwI	GGATC	5 cut(s)	528, 1041, 1133, 2048, 2061
AlwNI	CAGNNNCTG	2 cut(s)	61, 1544
Aor13HI	TCCGGA	1 cut(s)	86
AoxI	GGCC	9 cut(s)	3, 298, 399, 596, 1117, 1330, 1658, 2091, 2101
ApeKI	GCWGC	5 cut(s)	137, 173, 239, 650, 1688
ApoI	RAATTY	3 cut(s)	1779, 1896, 2115
AseI	ATTAAT	1 cut(s)	731
Asp700I	GAANNNNTTC	1 cut(s)	933
AspLEI	GCGC	2 cut(s)	640, 1724
AspS9I	GGNCC	4 cut(s)	356, 390, 1330, 1355
AsuC2I	CCSGG	1 cut(s)	1339
AsuHPI	GGTGA	2 cut(s)	1619, 1757
AsuNHI	GCTAGC	1 cut(s)	130
AvaII	GGWCC	3 cut(s)	356, 390, 1355
BalI	TGGCCA	1 cut(s)	1660
BamHI	GGATCC	1 cut(s)	2053
BanI	GGYRCC	1 cut(s)	1721
BanII	GRGCYC	1 cut(s)	794
Bbv12I	GWGCWC	2 cut(s)	276, 794
BbvI	GCAGC	5 cut(s)	124, 185, 226, 637, 1700
BccI	CCATC	5 cut(s)	49, 431, 805, 1307, 1417
BceAI	ACGGC	1 cut(s)	1709
BcgI	CGANNNNNNTGC	2 cut(s)	863, 897
BciT130I	CCWGG	6 cut(s)	429, 1605, 1865, 1881, 1902, 2090
BciVI	GTATCC	1 cut(s)	2073
BcnI	CCSGG	1 cut(s)	1339
BcoDI	GTCTC	7 cut(s)	239, 587, 818, 992, 1552, 1590, 1959
BfaI	CTAG	6 cut(s)	131, 542, 1047, 1284, 1692, 2076
BfmI	CTRYAG	2 cut(s)	240, 743
BfoI	RGCGCY	1 cut(s)	1725
BfrI	CTTAAG	1 cut(s)	1790
BfuAI	ACCTGC	3 cut(s)	183, 736, 1896
BfuI	GTATCC	1 cut(s)	2073
BisI	GCNGC	9 cut(s)	135, 138, 174, 190, 240, 385, 651, 1117, 1689
BlsI	GCNGC	9 cut(s)	136, 139, 175, 191, 241, 386, 652, 1118, 1690
Bme1390I	CCNGG	7 cut(s)	429, 1339, 1605, 1865, 1881, 1902, 2090
Bme18I	GGWCC	3 cut(s)	356, 390, 1355
BmgBI	CACGTC	2 cut(s)	986, 1591
BmgT120I	GGNCC	4 cut(s)	356, 390, 1330, 1355
BmiI	GGNNCC	8 cut(s)	391, 392, 1331, 1356, 1404, 1702, 1723, 2055
BmrFI	CCNGG	7 cut(s)	429, 1339, 1605, 1865, 1881, 1902, 2090
BmrI	ACTGGG	2 cut(s)	1283, 1397
BmsI	GCATC	6 cut(s)	274, 472, 586, 893, 899, 974
BmtI	GCTAGC	1 cut(s)	134
BmuI	ACTGGG	2 cut(s)	1283, 1397
BplI	GAGNNNNNCTC	4 cut(s)	244, 276, 1768, 1800
Bpu10I	CCTNAGC	2 cut(s)	302, 330
BpuMI	CCSGG	1 cut(s)	1339
BsaBI	GATNNNNATC	1 cut(s)	964
BsaHI	GRCGYC	3 cut(s)	65, 233, 1722
BsaI	GGTCTC	1 cut(s)	239
BsaJI	CCNNGG	3 cut(s)	516, 1275, 1407
BsaWI	WCCGGW	2 cut(s)	86, 1629
BsaXI	ACNNNNNCTCC	2 cut(s)	263, 293
Bsc4I	CCNNNNNNNGG	6 cut(s)	383, 517, 1132, 1352, 1550, 1636
Bse1I	ACTGG	6 cut(s)	448, 1023, 1177, 1191, 1289, 1392
Bse8I	GATNNNNATC	1 cut(s)	964
BseAI	TCCGGA	1 cut(s)	86
BseBI	CCWGG	6 cut(s)	429, 1605, 1865, 1881, 1902, 2090
BseDI	CCNNGG	3 cut(s)	516, 1275, 1407
BseGI	GGATG	6 cut(s)	41, 77, 83, 1437, 1459, 1873
BseJI	GATNNNNATC	1 cut(s)	964
BseLI	CCNNNNNNNGG	6 cut(s)	383, 517, 1132, 1352, 1550, 1636
BseMII	CTCAG	6 cut(s)	192, 293, 779, 784, 1341, 1430
BseNI	ACTGG	6 cut(s)	448, 1023, 1177, 1191, 1289, 1392
BseRI	GAGGAG	2 cut(s)	284, 399
BseXI	GCAGC	5 cut(s)	124, 185, 226, 637, 1700
BseYI	CCCAGC	1 cut(s)	717
BsgI	GTGCAG	1 cut(s)	1557
Bsh1236I	CGCG	2 cut(s)	124, 873
BshFI	GGCC	9 cut(s)	5, 300, 401, 598, 1119, 1332, 1660, 2093, 2103
BshNI	GGYRCC	1 cut(s)	1721
BsiHKAI	GWGCWC	2 cut(s)	276, 794
BsiSI	CCGG	4 cut(s)	12, 87, 1338, 1630
BslFI	GGGAC	3 cut(s)	376, 700, 843
BslI	CCNNNNNNNGG	6 cut(s)	383, 517, 1132, 1352, 1550, 1636
BsmAI	GTCTC	7 cut(s)	239, 587, 818, 992, 1552, 1590, 1959
BsmBI	CGTCTC	3 cut(s)	587, 818, 992
BsmFI	GGGAC	3 cut(s)	376, 700, 843
BsmI	GAATGC	1 cut(s)	1511
BsnI	GGCC	9 cut(s)	5, 300, 401, 598, 1119, 1332, 1660, 2093, 2103
Bso31I	GGTCTC	1 cut(s)	239
Bsp1286I	GDGCHC	2 cut(s)	276, 794
Bsp13I	TCCGGA	1 cut(s)	86
Bsp1407I	TGTACA	1 cut(s)	1870
Bsp143I	GATC	9 cut(s)	92, 108, 520, 874, 959, 1033, 1138, 1501, 2053
Bsp19I	CCATGG	1 cut(s)	1407
BspANI	GGCC	9 cut(s)	5, 300, 401, 598, 1119, 1332, 1660, 2093, 2103
BspCNI	CTCAG	6 cut(s)	193, 294, 780, 783, 1342, 1429
BspEI	TCCGGA	1 cut(s)	86
BspFNI	CGCG	2 cut(s)	124, 873
BspLI	GGNNCC	8 cut(s)	391, 392, 1331, 1356, 1404, 1702, 1723, 2055
BspMAI	CTGCAG	2 cut(s)	244, 747
BspMI	ACCTGC	3 cut(s)	183, 736, 1896
BspOI	GCTAGC	1 cut(s)	134
BspPI	GGATC	5 cut(s)	528, 1041, 1133, 2048, 2061
BspQI	GCTCTTC	2 cut(s)	285, 1629
BspT107I	GGYRCC	1 cut(s)	1721
BspTI	CTTAAG	1 cut(s)	1790
BspTNI	GGTCTC	1 cut(s)	239
BsrGI	TGTACA	1 cut(s)	1870
BsrI	ACTGG	6 cut(s)	448, 1023, 1177, 1191, 1289, 1392
BssECI	CCNNGG	3 cut(s)	516, 1275, 1407
BssMI	GATC	9 cut(s)	92, 108, 520, 874, 959, 1033, 1138, 1501, 2053
BssNI	GRCGYC	3 cut(s)	65, 233, 1722
BssT1I	CCWWGG	2 cut(s)	516, 1407
Bst2UI	CCWGG	6 cut(s)	429, 1605, 1865, 1881, 1902, 2090
Bst4CI	ACNGT	7 cut(s)	353, 565, 856, 1159, 1279, 1668, 1989
Bst6I	CTCTTC	3 cut(s)	285, 1133, 1629
BstACI	GRCGYC	3 cut(s)	65, 233, 1722
BstAFI	CTTAAG	1 cut(s)	1790
BstAPI	GCANNNNNTGC	1 cut(s)	635
BstAUI	TGTACA	1 cut(s)	1870
BstC8I	GCNNGC	7 cut(s)	34, 38, 132, 258, 327, 503, 631
BstDEI	CTNAG	7 cut(s)	201, 302, 330, 770, 788, 1350, 1416
BstDSI	CCRYGG	2 cut(s)	1275, 1407
BstF5I	GGATG	6 cut(s)	41, 77, 83, 1437, 1459, 1873
BstFNI	CGCG	2 cut(s)	124, 873
BstH2I	RGCGCY	1 cut(s)	1725
BstHHI	GCGC	2 cut(s)	640, 1724
BstKTI	GATC	9 cut(s)	95, 111, 523, 877, 962, 1036, 1141, 1504, 2056
BstMAI	GTCTC	7 cut(s)	239, 587, 818, 992, 1552, 1590, 1959
BstMBI	GATC	9 cut(s)	92, 108, 520, 874, 959, 1033, 1138, 1501, 2053
BstMWI	GCNNNNNNNGC	5 cut(s)	393, 460, 635, 695, 1001
BstNI	CCWGG	6 cut(s)	429, 1605, 1865, 1881, 1902, 2090
BstNSI	RCATGY	2 cut(s)	633, 1384
BstSCI	CCNGG	7 cut(s)	427, 1337, 1603, 1863, 1879, 1900, 2088
BstSFI	CTRYAG	2 cut(s)	240, 743
BstUI	CGCG	2 cut(s)	124, 873
BstV1I	GCAGC	5 cut(s)	124, 185, 226, 637, 1700
BstX2I	RGATCY	2 cut(s)	1138, 2053
BstYI	RGATCY	2 cut(s)	1138, 2053
BsuI	GTATCC	1 cut(s)	2073
BsuRI	GGCC	9 cut(s)	5, 300, 401, 598, 1119, 1332, 1660, 2093, 2103
BtgI	CCRYGG	2 cut(s)	1275, 1407
BtrI	CACGTC	2 cut(s)	986, 1591
BtsCI	GGATG	6 cut(s)	41, 77, 83, 1437, 1459, 1873
BtsIMutI	CAGTG	3 cut(s)	441, 1030, 1296
BveI	ACCTGC	3 cut(s)	183, 736, 1896
Cac8I	GCNNGC	7 cut(s)	34, 38, 132, 258, 327, 503, 631
CaiI	CAGNNNCTG	2 cut(s)	61, 1544
CfoI	GCGC	2 cut(s)	640, 1724
Cfr13I	GGNCC	4 cut(s)	356, 390, 1330, 1355
CseI	GACGC	1 cut(s)	129
Csp6I	GTAC	3 cut(s)	1194, 1712, 1871
CviAII	CATG	5 cut(s)	630, 1199, 1381, 1408, 1982
CviQI	GTAC	3 cut(s)	1194, 1712, 1871
DdeI	CTNAG	7 cut(s)	201, 302, 330, 770, 788, 1350, 1416
DinI	GGCGCC	1 cut(s)	1723
DpnI	GATC	9 cut(s)	94, 110, 522, 876, 961, 1035, 1140, 1503, 2055
DpnII	GATC	9 cut(s)	92, 108, 520, 874, 959, 1033, 1138, 1501, 2053
DrdI	GACNNNNNNGTC	2 cut(s)	70, 232
DseDI	GACNNNNNNGTC	2 cut(s)	70, 232
EaeI	YGGCCR	3 cut(s)	399, 1117, 1658
Eam1104I	CTCTTC	3 cut(s)	285, 1133, 1629
EarI	CTCTTC	3 cut(s)	285, 1133, 1629
Ecl136II	GAGCTC	1 cut(s)	792
Eco130I	CCWWGG	2 cut(s)	516, 1407
Eco147I	AGGCCT	3 cut(s)	300, 598, 2093
Eco24I	GRGCYC	1 cut(s)	794
Eco31I	GGTCTC	1 cut(s)	239
Eco32I	GATATC	2 cut(s)	916, 2131
Eco47I	GGWCC	3 cut(s)	356, 390, 1355
Eco53kI	GAGCTC	1 cut(s)	792
Eco57I	CTGAAG	2 cut(s)	691, 1218
EcoICRI	GAGCTC	1 cut(s)	792
EcoO109I	RGGNCCY	3 cut(s)	390, 1330, 1355
EcoRI	GAATTC	1 cut(s)	1779
EcoRII	CCWGG	6 cut(s)	427, 1603, 1863, 1879, 1900, 2088
EcoRV	GATATC	2 cut(s)	916, 2131
EcoT14I	CCWWGG	2 cut(s)	516, 1407
EcoT38I	GRGCYC	1 cut(s)	794
EgeI	GGCGCC	1 cut(s)	1723
EheI	GGCGCC	1 cut(s)	1723
ErhI	CCWWGG	2 cut(s)	516, 1407
Esp3I	CGTCTC	3 cut(s)	587, 818, 992
FaeI	CATG	5 cut(s)	633, 1202, 1384, 1411, 1985
FalI	AAGNNNNNCTT	2 cut(s)	302, 334
FaqI	GGGAC	3 cut(s)	376, 700, 843
FatI	CATG	5 cut(s)	629, 1198, 1380, 1407, 1981
FauI	CCCGC	4 cut(s)	37, 41, 380, 401
FauNDI	CATATG	1 cut(s)	973
Fnu4HI	GCNGC	9 cut(s)	135, 138, 174, 190, 240, 385, 651, 1117, 1689
FokI	GGATG	6 cut(s)	28, 64, 70, 1424, 1446, 1880
FriOI	GRGCYC	1 cut(s)	794
Fsp4HI	GCNGC	9 cut(s)	135, 138, 174, 190, 240, 385, 651, 1117, 1689
FspBI	CTAG	6 cut(s)	131, 542, 1047, 1284, 1692, 2076
GlaI	GCGC	2 cut(s)	639, 1723
GluI	GCNGC	9 cut(s)	135, 138, 174, 190, 240, 385, 651, 1117, 1689
GsaI	CCCAGC	1 cut(s)	721
HaeII	RGCGCY	1 cut(s)	1725
HaeIII	GGCC	9 cut(s)	5, 300, 401, 598, 1119, 1332, 1660, 2093, 2103
HapII	CCGG	4 cut(s)	12, 87, 1338, 1630
HgaI	GACGC	1 cut(s)	129
HhaI	GCGC	2 cut(s)	640, 1724
Hin1I	GRCGYC	3 cut(s)	65, 233, 1722
Hin1II	CATG	5 cut(s)	633, 1202, 1384, 1411, 1985
Hin6I	GCGC	2 cut(s)	638, 1722
HinP1I	GCGC	2 cut(s)	638, 1722
HincII	GTYRAC	3 cut(s)	144, 1272, 1812
HindII	GTYRAC	3 cut(s)	144, 1272, 1812
HinfI	GANTC	9 cut(s)	115, 163, 404, 617, 758, 1096, 1256, 1342, 1884
HpaI	GTTAAC	2 cut(s)	1272, 1812
HpaII	CCGG	4 cut(s)	12, 87, 1338, 1630
HphI	GGTGA	2 cut(s)	1619, 1757
Hpy166II	GTNNAC	7 cut(s)	144, 356, 1028, 1272, 1748, 1812, 1873
Hpy188III	TCNNGA	6 cut(s)	56, 87, 112, 266, 755, 1499
Hpy8I	GTNNAC	7 cut(s)	144, 356, 1028, 1272, 1748, 1812, 1873
HpyCH4III	ACNGT	7 cut(s)	353, 565, 856, 1159, 1279, 1668, 1989
HpyCH4IV	ACGT	6 cut(s)	65, 233, 701, 985, 1590, 2066
HpyCH4V	TGCA	7 cut(s)	242, 505, 745, 884, 1085, 1538, 1556
HpyF10VI	GCNNNNNNNGC	5 cut(s)	393, 460, 635, 695, 1001
HpyF3I	CTNAG	7 cut(s)	201, 302, 330, 770, 788, 1350, 1416
HpySE526I	ACGT	6 cut(s)	65, 233, 701, 985, 1590, 2066
Hsp92I	GRCGYC	3 cut(s)	65, 233, 1722
Hsp92II	CATG	5 cut(s)	633, 1202, 1384, 1411, 1985
HspAI	GCGC	2 cut(s)	638, 1722
KasI	GGCGCC	1 cut(s)	1721
KflI	GGGWCCC	1 cut(s)	390
Kpn2I	TCCGGA	1 cut(s)	86
KspAI	GTTAAC	2 cut(s)	1272, 1812
Kzo9I	GATC	9 cut(s)	92, 108, 520, 874, 959, 1033, 1138, 1501, 2053
LguI	GCTCTTC	2 cut(s)	285, 1629
LmnI	GCTCC	5 cut(s)	271, 350, 626, 1408, 1700
Lsp1109I	GCAGC	5 cut(s)	124, 185, 226, 637, 1700
LweI	GCATC	6 cut(s)	274, 472, 586, 893, 899, 974
MaeI	CTAG	6 cut(s)	131, 542, 1047, 1284, 1692, 2076
MaeII	ACGT	6 cut(s)	65, 233, 701, 985, 1590, 2066
MaeIII	GTNAC	8 cut(s)	570, 815, 1041, 1153, 1207, 1279, 1383, 1411
MalI	GATC	9 cut(s)	94, 110, 522, 876, 961, 1035, 1140, 1503, 2055
MboI	GATC	9 cut(s)	92, 108, 520, 874, 959, 1033, 1138, 1501, 2053
MboII	GAAGA	8 cut(s)	102, 302, 869, 1120, 1148, 1330, 1616, 1745
MfeI	CAATTG	1 cut(s)	1932
MflI	RGATCY	2 cut(s)	1138, 2053
MhlI	GDGCHC	2 cut(s)	276, 794
MlsI	TGGCCA	1 cut(s)	1660
MluNI	TGGCCA	1 cut(s)	1660
Mly113I	GGCGCC	1 cut(s)	1722
MlyI	GAGTC	1 cut(s)	752
MmeI	TCCRAC	6 cut(s)	182, 372, 592, 1650, 1708, 1986
Mox20I	TGGCCA	1 cut(s)	1660
MroI	TCCGGA	1 cut(s)	86
MroXI	GAANNNNTTC	1 cut(s)	933
MscI	TGGCCA	1 cut(s)	1660
MseI	TTAA	5 cut(s)	657, 731, 1271, 1791, 1811
MslI	CAYNNNNRTG	2 cut(s)	937, 1442
Msp20I	TGGCCA	1 cut(s)	1660
MspA1I	CMGCKG	3 cut(s)	137, 239, 1116
MspCI	CTTAAG	1 cut(s)	1790
MspI	CCGG	4 cut(s)	12, 87, 1338, 1630
MspR9I	CCNGG	7 cut(s)	429, 1339, 1605, 1865, 1881, 1902, 2090
MunI	CAATTG	1 cut(s)	1932
Mva1269I	GAATGC	1 cut(s)	1511
MvaI	CCWGG	6 cut(s)	429, 1605, 1865, 1881, 1902, 2090
MvnI	CGCG	2 cut(s)	124, 873
MwoI	GCNNNNNNNGC	5 cut(s)	393, 460, 635, 695, 1001
NarI	GGCGCC	1 cut(s)	1722
NciI	CCSGG	1 cut(s)	1339
NcoI	CCATGG	1 cut(s)	1407
NdeI	CATATG	1 cut(s)	973
NdeII	GATC	9 cut(s)	92, 108, 520, 874, 959, 1033, 1138, 1501, 2053
NheI	GCTAGC	1 cut(s)	130
NlaIII	CATG	5 cut(s)	633, 1202, 1384, 1411, 1985
NlaIV	GGNNCC	8 cut(s)	391, 392, 1331, 1356, 1404, 1702, 1723, 2055
NmuCI	GTSAC	2 cut(s)	570, 1279
NspI	RCATGY	2 cut(s)	633, 1384
OliI	CACNNNNGTG	1 cut(s)	937
PaeI	GCATGC	1 cut(s)	633
PceI	AGGCCT	3 cut(s)	300, 598, 2093
PciI	ACATGT	1 cut(s)	1380
PciSI	GCTCTTC	2 cut(s)	285, 1629
PcsI	WCGNNNNNNNCGW	3 cut(s)	224, 227, 230
PctI	GAATGC	1 cut(s)	1511
PdmI	GAANNNNTTC	1 cut(s)	933
PfeI	GAWTC	8 cut(s)	115, 163, 404, 617, 1096, 1256, 1342, 1884
PflFI	GACNNNGTC	2 cut(s)	67, 232
PkrI	GCNGC	9 cut(s)	136, 139, 175, 191, 241, 386, 652, 1118, 1690
PleI	GAGTC	1 cut(s)	752
PluTI	GGCGCC	1 cut(s)	1725
PpsI	GAGTC	1 cut(s)	752
PpuMI	RGGWCCY	2 cut(s)	390, 1355
PscI	ACATGT	1 cut(s)	1380
PshBI	ATTAAT	1 cut(s)	731
PsiI	TTATAA	1 cut(s)	1475
Psp124BI	GAGCTC	1 cut(s)	794
Psp1406I	AACGTT	1 cut(s)	2066
Psp5II	RGGWCCY	2 cut(s)	390, 1355
Psp6I	CCWGG	6 cut(s)	427, 1603, 1863, 1879, 1900, 2088
PspFI	CCCAGC	1 cut(s)	717
PspGI	CCWGG	6 cut(s)	427, 1603, 1863, 1879, 1900, 2088
PspN4I	GGNNCC	8 cut(s)	391, 392, 1331, 1356, 1404, 1702, 1723, 2055
PspPI	GGNCC	4 cut(s)	356, 390, 1330, 1355
PspPPI	RGGWCCY	2 cut(s)	390, 1355
PsrI	GAACNNNNNNTAC	2 cut(s)	1977, 2009
PstI	CTGCAG	2 cut(s)	244, 747
PstNI	CAGNNNCTG	2 cut(s)	61, 1544
PsuI	RGATCY	2 cut(s)	1138, 2053
PsyI	GACNNNGTC	2 cut(s)	67, 232
PvuII	CAGCTG	1 cut(s)	239
RsaI	GTAC	3 cut(s)	1195, 1713, 1872
RsaNI	GTAC	3 cut(s)	1194, 1712, 1871
RseI	CAYNNNNRTG	2 cut(s)	937, 1442
SacI	GAGCTC	1 cut(s)	794
SapI	GCTCTTC	2 cut(s)	285, 1629
SaqAI	TTAA	5 cut(s)	657, 731, 1271, 1791, 1811
SatI	GCNGC	9 cut(s)	135, 138, 174, 190, 240, 385, 651, 1117, 1689
Sau3AI	GATC	9 cut(s)	92, 108, 520, 874, 959, 1033, 1138, 1501, 2053
Sau96I	GGNCC	4 cut(s)	356, 390, 1330, 1355
SchI	GAGTC	1 cut(s)	752
ScrFI	CCNGG	7 cut(s)	429, 1339, 1605, 1865, 1881, 1902, 2090
SduI	GDGCHC	2 cut(s)	276, 794
SfaNI	GCATC	6 cut(s)	274, 472, 586, 893, 899, 974
SfcI	CTRYAG	2 cut(s)	240, 743
SfoI	GGCGCC	1 cut(s)	1723
SinI	GGWCC	3 cut(s)	356, 390, 1355
SmiMI	CAYNNNNRTG	2 cut(s)	937, 1442
SmlI	CTYRAG	1 cut(s)	1790
SmoI	CTYRAG	1 cut(s)	1790
SphI	GCATGC	1 cut(s)	633
SseBI	AGGCCT	3 cut(s)	300, 598, 2093
SspDI	GGCGCC	1 cut(s)	1721
SspMI	CTAG	6 cut(s)	131, 542, 1047, 1284, 1692, 2076
SstI	GAGCTC	1 cut(s)	794
StuI	AGGCCT	3 cut(s)	300, 598, 2093
StyD4I	CCNGG	7 cut(s)	427, 1337, 1603, 1863, 1879, 1900, 2088
StyI	CCWWGG	2 cut(s)	516, 1407
TaaI	ACNGT	7 cut(s)	353, 565, 856, 1159, 1279, 1668, 1989
TaiI	ACGT	6 cut(s)	68, 236, 704, 988, 1593, 2069
TaqI	TCGA	8 cut(s)	95, 113, 282, 321, 489, 523, 1515, 2127
TaqII	GACCGA	1 cut(s)	558
TatI	WGTACW	1 cut(s)	1870
TauI	GCSGC	4 cut(s)	137, 192, 387, 1119
TfiI	GAWTC	8 cut(s)	115, 163, 404, 617, 1096, 1256, 1342, 1884
Tru1I	TTAA	5 cut(s)	657, 731, 1271, 1791, 1811
Tru9I	TTAA	5 cut(s)	657, 731, 1271, 1791, 1811
TscAI	CASTG	3 cut(s)	448, 1030, 1296
TseFI	GTSAC	2 cut(s)	570, 1279
TseI	GCWGC	5 cut(s)	137, 173, 239, 650, 1688
Tsp45I	GTSAC	2 cut(s)	570, 1279
TspDTI	ATGAA	2 cut(s)	328, 1998
TspGWI	ACGGA	4 cut(s)	34, 41, 108, 2046
TspRI	CASTG	3 cut(s)	448, 1030, 1296
Tth111I	GACNNNGTC	2 cut(s)	67, 232
Vha464I	CTTAAG	1 cut(s)	1790
VpaK11BI	GGWCC	3 cut(s)	356, 390, 1355
VspI	ATTAAT	1 cut(s)	731
XapI	RAATTY	3 cut(s)	1779, 1896, 2115
XceI	RCATGY	2 cut(s)	633, 1384
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2096
XmnI	GAANNNNTTC	1 cut(s)	933
XspI	CTAG	6 cut(s)	131, 542, 1047, 1284, 1692, 2076
ZraI	GACGTC	2 cut(s)	66, 234

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.