Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0000740

Physical Location & Seq

Genomic Coordinates Reverse (-)

79321753 .. 79326680

Gene Structure 4928 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG463400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2064 bp

ATGTCCTGCAACGCACTTTGTCTCTTTCCCTGCCTCTTTCCTCCTTCCATTTTCCGACATGAATTGACGTCTTCACCATCAGGAAGGTCCAAGTTAACTGCCTTGTTCTCTATTAATCCATCTAGGACTAGGAGGTTAGCCGTCTCCACACACCACACTCCAACCTTGACGTCTCGGATAACGGGAGCTATGGAGGATCATGATCATCGTCATCGACATGTAGAGGACGAGCGGGTGAGCTTCAAACATTTTATTGAGCAAGCTAAGGATCTTGTCAGACCAGACAGCAGTACTTCTGCACCGTCACCTCGCTGGTTCTCACCTTTAGACTGCGGCCCCCCATTGGACAATTCCCCCCTGCTTCTCTTTTTGCCCGGAATTGATGGCACCGGACTTGGGCTTATAATGCATCACAAGAAACTAGGAAAGATTTTCGAGGTCTGGTGCTTGCACATCCCTCCTAAAGATCGAACACCATTTACAGACTTAGTGAAGCTAATTGAGAGCACAATTCGGTCAGAGCATCATTGCTCACCTAATAGACCAATATATCTTGTTGGAGAATCGCTTGGAGCATGCCTTGCACTATCTGTGGCAGCCTTTAACCCTGACATCGACCTTGTTCTAATTTTAGCAAATCCAGCAACATCCTTCAGCAGGTCACAACTTCAACCCATATTACATTTACTGCAGTTCATGCCTGACTCACCCAATCTTAGCCTTCTTCATATACTCAGTTCAATGACTGGTGCAATGATGGATAATTTGGAGAAGGGAATTGAAAGGCTATCGCCGGATCTTGTTGCACCATCAATAGCTTACCTCTCTGTTATTGCTGATATCTTACCCATGGAAACACTTGTATGGAAGTTACAAATGATTAGTTCAGCTTCTTCTTATGCCAATTCACACCTCCATGCTGTGAAGGCTCAAACCCTGATACTTTCCAGCGGAAGGGATCCATTGCTACCTAGTCATGAAGAAGGTCCGAGACTACGATGGCTGCTGGCAAATTGTGAAATCTGTACTTTTGAAGACTGCGGTCATTTCCTCTTCTTGGAAGCTGCTCATGATTTGGTAACTGTCATCAAGCAGGTTGGTGTCTATCGTCGTTCAAAAGAAAGAGACTTTGTTTCAGATTACATACCACTATCACCTTCTGAATTAAAAAATGTATTGGACAAACACAGATGGATTAGCACAGTCATGAGCCCAGTTATACTCTCAACTTTAGAAGATGGGAAGATAGTGAGGGGCTTTGCCGGGATTCCTTTAGAGGGCCCTGTAGTGTATGTTGGTTATCACATGTTACTGGGATCTGAACTCTTTCCATTGGTAACTCTGTTTTCGGAACAAAAAAACATCCCTCTGAGAGGTTTAGCACATCCAGTGATGTTTATGAAATTGAATCAAGGATGGTTCCCTGAGCATATGTTCGACGATCTCAAACATGCGGGGGCTGTTCCTGTCTCCGGAAAAAACTTTTTTAAGCTTCTCTCTACAAAGTCTCATTTCCTACTCTTTCCTGGTGGATTGCGGGAGGCTTTACACCGGAAGGGGGAAGCATATAAGTTATTCTGGCCAGAAAGGGCAGAGTTTGTGAGGATGGCAGCTAGATTTGGCGCGAAGATTGTACCTTTTGGTACAGTTGGAGAGGATGATTTTGCTGACATATTATTCGATTATGAAGACCAGATGAAAATTCCATTCTTCAAGAAATATATAGATGAATTATCGGATAAATTAGCTGGAAAAGTGAGGGCAACTTCTGAGGGGGAGGTAGGAAACCAAGCTTTATACTTGCCAGGAATTCTACCAAAATTTCCTGGCCGGTTTTATCATCATTTTGGGAAACCAATTGAAACGGAAGGGAGGAAGGAGGAATTACGAGACAAGGAAAATGCTCATGAATTGTATTTACAGGTGAAGTTAGAGGTTGAGAACTGCCTTGCTTATTTGCAGGAAAAAAGAGAGAGTGATCCATACAGAAGCCTTGTGTCTCGGATACTATACCAAGCCAAAAATGGCTTTACAGCTCAAGTTCCTACATTTGACGTGGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010287 GO:0016101 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0019432 GO:0033306 GO:0034308 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:1901576 GO:1901615 GO:1903173

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6 DAGAT Hydrolase_4

Protein Analysis

687

Amino Acids

77.33

Weight (kDa)

6.67

Isoelectric Point (pI)

51.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_6	PF12697	153 - 360	1.2e-08	Alpha/beta hydrolase family
Abhydrolase_1	PF00561	159 - 355	1.4e-07	alpha/beta hydrolase fold
Hydrolase_4	PF12146	162 - 354	1.8e-06	Serine aminopeptidase, S33
DAGAT	PF03982	484 - 557	5.6e-07	Diacylglycerol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000740)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02030 AT3G02030 AT3G26820 AT3G26820 AT3G26840 AT5G41120 AT5G41120 AT5G41120 AT5G41130 AT5G41130 AT5G41130
fragaria_vesca	FvH4_3g35400 FvH4_3g39220 FvH4_3g39220 FvH4_3g39270 FvH4_3g39280 FvH4_3g39280 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060
malus_domestica	MD09G1005800.v1.1 MD17G1009900.v1.1
prunus_persica	Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1
pyrus_communis	pycom111g00420
rosa_chinensis	RchiOBHm_Chr2g0176241 RchiOBHm_Chr5g0070671 RchiOBHm_Chr5g0070681 RchiOBHm_Chr5g0070691
rosa_laevigata	RLG00000022394 RLG00000036149 RLG00000036150 RLG00000036152
rosa_multiflora	Rmu_co8466433.1_g000001 Rmu_sc0000249.1_g000001 Rmu_sc0000405.1_g000016 Rmu_sc0005197.1_g000009 Rmu_sc0005197.1_g000015 Rmu_sc0036323.1_g000001
rosa_roxburghii	Rroxscaffold_1G00010480 Rroxscaffold_1G00010530 Rroxscaffold_1G00010550 Rroxscaffold_1G00010560 Rroxscaffold_2G00076650 Rroxscaffold_5G00357250
rosa_rugosa	Rorug02G0592200 Rorug02G0592300 Rorug05G0407500 Rorug05G0407800
rosa_samantha	Rh2AG670700 Rh2BG682200 Rh2CG645000 Rh2DG695800 Rh5AG463200 Rh5AG463300 Rh5AG463400 Rh5BG481200 Rh5CG505400 Rh5CG505500 Rh5DG493300 Rh5DG493600 Rh5DG493700
rosa_wichuraiana	Rw2G055020 Rw5G043110 Rw5G043120 Rw5G043130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	404
AatII	GACGTC	2 cut(s)	71, 173
Acc36I	ACCTGC	2 cut(s)	648, 1084
AccB1I	GGYRCC	1 cut(s)	386
AccBSI	CCGCTC	1 cut(s)	232
AccII	CGCG	1 cut(s)	1625
AccIII	TCCGGA	1 cut(s)	1472
AciI	CCGC	6 cut(s)	232, 333, 951, 1041, 1454, 1537
AclWI	GGATC	7 cut(s)	204, 276, 804, 953, 966, 1324, 1973
AcoI	YGGCCR	2 cut(s)	1580, 1828
AcsI	RAATTY	3 cut(s)	1701, 1809, 1820
AcuI	CTGAAG	1 cut(s)	637
AcyI	GRCGYC	2 cut(s)	68, 170
AfaI	GTAC	4 cut(s)	292, 1027, 1635, 1645
AfiI	CCNNNNNNNGG	9 cut(s)	343, 396, 657, 954, 1057, 1277, 1373, 1472, 1558
AflIII	ACRYGT	2 cut(s)	217, 1305
AgsI	TTSAA	9 cut(s)	244, 671, 741, 782, 1034, 1116, 1408, 1714, 1862
AjiI	CACGTC	1 cut(s)	2056
AjnI	CCWGG	3 cut(s)	1525, 1804, 1825
Alw21I	GWGCWC	1 cut(s)	509
Alw26I	GTCTC	9 cut(s)	26, 148, 177, 985, 1119, 1474, 1512, 1884, 2004
AlwI	GGATC	7 cut(s)	204, 276, 804, 953, 966, 1324, 1973
Aor13HI	TCCGGA	1 cut(s)	1472
AoxI	GGCC	4 cut(s)	334, 1279, 1580, 1828
ApaI	GGGCCC	1 cut(s)	1283
ApeKI	GCWGC	4 cut(s)	596, 1003, 1064, 1610
ApoI	RAATTY	3 cut(s)	1701, 1809, 1820
ArsI	GACNNNNNNTTYG	2 cut(s)	1661, 1693
AseI	ATTAAT	1 cut(s)	114
Asp700I	GAANNNNTTC	1 cut(s)	1326
AspLEI	GCGC	1 cut(s)	1625
AspS9I	GGNCC	5 cut(s)	87, 335, 986, 1279, 1280
AsuC2I	CCSGG	2 cut(s)	375, 1264
AsuHPI	GGTGA	8 cut(s)	66, 247, 297, 312, 525, 699, 1146, 1936
AvaII	GGWCC	2 cut(s)	87, 986
BaeGI	GKGCMC	1 cut(s)	1283
BalI	TGGCCA	1 cut(s)	1582
BamHI	GGATCC	1 cut(s)	958
BanI	GGYRCC	1 cut(s)	386
BanII	GRGCYC	2 cut(s)	1214, 1283
BbsI	GAAGAC	3 cut(s)	63, 1041, 1695
Bbv12I	GWGCWC	1 cut(s)	509
BbvI	GCAGC	4 cut(s)	608, 990, 1051, 1622
BceAI	ACGGC	1 cut(s)	125
BciT130I	CCWGG	3 cut(s)	1527, 1806, 1827
BciVI	GTATCC	1 cut(s)	1998
BclI	TGATCA	1 cut(s)	202
BcnI	CCSGG	2 cut(s)	375, 1264
BcoDI	GTCTC	9 cut(s)	26, 148, 177, 985, 1119, 1474, 1512, 1884, 2004
BfaI	CTAG	5 cut(s)	123, 129, 422, 972, 1614
BfmI	CTRYAG	2 cut(s)	689, 1284
BfuAI	ACCTGC	2 cut(s)	648, 1084
BfuI	GTATCC	1 cut(s)	1998
BisI	GCNGC	5 cut(s)	334, 597, 1004, 1065, 1611
BlsI	GCNGC	5 cut(s)	335, 598, 1005, 1066, 1612
BmcAI	AGTACT	1 cut(s)	292
Bme1390I	CCNGG	5 cut(s)	375, 1264, 1527, 1806, 1827
Bme18I	GGWCC	2 cut(s)	87, 986
BmgBI	CACGTC	1 cut(s)	2056
BmgT120I	GGNCC	5 cut(s)	87, 335, 986, 1279, 1280
BmiI	GGNNCC	5 cut(s)	337, 388, 960, 1281, 1421
BmrFI	CCNGG	5 cut(s)	375, 1264, 1527, 1806, 1827
BmrI	ACTGGG	2 cut(s)	1208, 1322
BmsI	GCATC	2 cut(s)	418, 532
BmuI	ACTGGG	2 cut(s)	1208, 1322
BoxI	GACNNNNGTC	1 cut(s)	1041
BpiI	GAAGAC	3 cut(s)	63, 1041, 1695
Bpu10I	CCTNAGC	2 cut(s)	264, 1425
BpuEI	CTTGAG	1 cut(s)	2022
BpuMI	CCSGG	2 cut(s)	375, 1264
BsaBI	GATNNNNATC	1 cut(s)	201
BsaHI	GRCGYC	2 cut(s)	68, 170
BsaJI	CCNNGG	1 cut(s)	849
BsaWI	WCCGGW	3 cut(s)	389, 1472, 1551
Bsc4I	CCNNNNNNNGG	9 cut(s)	343, 396, 657, 954, 1057, 1277, 1373, 1472, 1558
Bse118I	RCCGGY	1 cut(s)	1830
Bse1I	ACTGG	4 cut(s)	751, 1214, 1317, 1388
Bse3DI	GCAATG	3 cut(s)	526, 759, 962
Bse8I	GATNNNNATC	1 cut(s)	201
BseAI	TCCGGA	1 cut(s)	1472
BseBI	CCWGG	3 cut(s)	1527, 1806, 1827
BseDI	CCNNGG	1 cut(s)	849
BseGI	GGATG	7 cut(s)	453, 647, 1362, 1384, 1421, 1611, 1663
BseJI	GATNNNNATC	1 cut(s)	201
BseLI	CCNNNNNNNGG	9 cut(s)	343, 396, 657, 954, 1057, 1277, 1373, 1472, 1558
BseMI	GCAATG	3 cut(s)	526, 759, 962
BseMII	CTCAG	4 cut(s)	748, 1361, 1416, 1761
BseNI	ACTGG	4 cut(s)	751, 1214, 1317, 1388
BseSI	GKGCMC	1 cut(s)	1283
BseXI	GCAGC	4 cut(s)	608, 990, 1051, 1622
BsgI	GTGCAG	1 cut(s)	282
Bsh1236I	CGCG	1 cut(s)	1625
BshFI	GGCC	4 cut(s)	336, 1281, 1582, 1830
BshNI	GGYRCC	1 cut(s)	386
BsiHKAI	GWGCWC	1 cut(s)	509
BsiSI	CCGG	7 cut(s)	375, 390, 794, 1263, 1473, 1552, 1831
BslI	CCNNNNNNNGG	9 cut(s)	343, 396, 657, 954, 1057, 1277, 1373, 1472, 1558
BsmAI	GTCTC	9 cut(s)	26, 148, 177, 985, 1119, 1474, 1512, 1884, 2004
BsmBI	CGTCTC	2 cut(s)	148, 177
BsnI	GGCC	4 cut(s)	336, 1281, 1582, 1830
Bsp120I	GGGCCC	1 cut(s)	1279
Bsp1286I	GDGCHC	3 cut(s)	509, 1214, 1283
Bsp13I	TCCGGA	1 cut(s)	1472
Bsp143I	GATC	9 cut(s)	196, 202, 268, 466, 796, 958, 1316, 1441, 1978
Bsp19I	CCATGG	1 cut(s)	849
BspACI	CCGC	6 cut(s)	232, 333, 951, 1041, 1454, 1537
BspANI	GGCC	4 cut(s)	336, 1281, 1582, 1830
BspCNI	CTCAG	4 cut(s)	747, 1362, 1417, 1762
BspEI	TCCGGA	1 cut(s)	1472
BspFNI	CGCG	1 cut(s)	1625
BspHI	TCATGA	5 cut(s)	199, 976, 1069, 1206, 1906
BspLI	GGNNCC	5 cut(s)	337, 388, 960, 1281, 1421
BspMAI	CTGCAG	1 cut(s)	693
BspMI	ACCTGC	2 cut(s)	648, 1084
BspPI	GGATC	7 cut(s)	204, 276, 804, 953, 966, 1324, 1973
BspT107I	GGYRCC	1 cut(s)	386
BsrBI	CCGCTC	1 cut(s)	232
BsrDI	GCAATG	3 cut(s)	526, 759, 962
BsrFI	RCCGGY	1 cut(s)	1830
BsrI	ACTGG	4 cut(s)	751, 1214, 1317, 1388
BssAI	RCCGGY	1 cut(s)	1830
BssECI	CCNNGG	1 cut(s)	849
BssMI	GATC	9 cut(s)	196, 202, 268, 466, 796, 958, 1316, 1441, 1978
BssNI	GRCGYC	2 cut(s)	68, 170
BssT1I	CCWWGG	1 cut(s)	849
Bst2UI	CCWGG	3 cut(s)	1527, 1806, 1827
Bst4CI	ACNGT	4 cut(s)	303, 1084, 1204, 1648
Bst6I	CTCTTC	1 cut(s)	1058
BstACI	GRCGYC	2 cut(s)	68, 170
BstAPI	GCANNNNNTGC	2 cut(s)	581, 697
BstC8I	GCNNGC	4 cut(s)	261, 449, 577, 1008
BstDEI	CTNAG	7 cut(s)	264, 487, 716, 734, 1370, 1425, 1770
BstDSI	CCRYGG	1 cut(s)	849
BstENI	CCTNNNNNAGG	2 cut(s)	655, 1371
BstF5I	GGATG	7 cut(s)	453, 647, 1362, 1384, 1421, 1611, 1663
BstFNI	CGCG	1 cut(s)	1625
BstHHI	GCGC	1 cut(s)	1625
BstKTI	GATC	9 cut(s)	199, 205, 271, 469, 799, 961, 1319, 1444, 1981
BstMAI	GTCTC	9 cut(s)	26, 148, 177, 985, 1119, 1474, 1512, 1884, 2004
BstMBI	GATC	9 cut(s)	196, 202, 268, 466, 796, 958, 1316, 1441, 1978
BstMWI	GCNNNNNNNGC	5 cut(s)	406, 581, 641, 697, 926
BstNI	CCWGG	3 cut(s)	1527, 1806, 1827
BstNSI	RCATGY	4 cut(s)	221, 579, 1309, 1454
BstPAI	GACNNNNGTC	1 cut(s)	1041
BstSCI	CCNGG	5 cut(s)	373, 1262, 1525, 1804, 1825
BstSFI	CTRYAG	2 cut(s)	689, 1284
BstSLI	GKGCMC	1 cut(s)	1283
BstUI	CGCG	1 cut(s)	1625
BstV1I	GCAGC	4 cut(s)	608, 990, 1051, 1622
BstV2I	GAAGAC	3 cut(s)	63, 1041, 1695
BstX2I	RGATCY	4 cut(s)	268, 796, 958, 1316
BstYI	RGATCY	4 cut(s)	268, 796, 958, 1316
BsuI	GTATCC	1 cut(s)	1998
BsuRI	GGCC	4 cut(s)	336, 1281, 1582, 1830
BtgI	CCRYGG	1 cut(s)	849
BtrI	CACGTC	1 cut(s)	2056
BtsCI	GGATG	7 cut(s)	453, 647, 1362, 1384, 1421, 1611, 1663
BtsIMutI	CAGTG	1 cut(s)	1395
BveI	ACCTGC	2 cut(s)	648, 1084
Cac8I	GCNNGC	4 cut(s)	261, 449, 577, 1008
CciI	TCATGA	5 cut(s)	199, 976, 1069, 1206, 1906
CfoI	GCGC	1 cut(s)	1625
Cfr10I	RCCGGY	1 cut(s)	1830
Cfr13I	GGNCC	5 cut(s)	87, 335, 986, 1279, 1280
Csp6I	GTAC	4 cut(s)	291, 1026, 1634, 1644
CviQI	GTAC	4 cut(s)	291, 1026, 1634, 1644
DdeI	CTNAG	7 cut(s)	264, 487, 716, 734, 1370, 1425, 1770
DpnI	GATC	9 cut(s)	198, 204, 270, 468, 798, 960, 1318, 1443, 1980
DpnII	GATC	9 cut(s)	196, 202, 268, 466, 796, 958, 1316, 1441, 1978
EaeI	YGGCCR	2 cut(s)	1580, 1828
Eam1104I	CTCTTC	1 cut(s)	1058
EarI	CTCTTC	1 cut(s)	1058
Eco130I	CCWWGG	1 cut(s)	849
Eco24I	GRGCYC	2 cut(s)	1214, 1283
Eco32I	GATATC	1 cut(s)	841
Eco47I	GGWCC	2 cut(s)	87, 986
Eco57I	CTGAAG	1 cut(s)	637
EcoNI	CCTNNNNNAGG	2 cut(s)	655, 1371
EcoO109I	RGGNCCY	2 cut(s)	1279, 1280
EcoRI	GAATTC	1 cut(s)	1809
EcoRII	CCWGG	3 cut(s)	1525, 1804, 1825
EcoRV	GATATC	1 cut(s)	841
EcoT14I	CCWWGG	1 cut(s)	849
EcoT22I	ATGCAT	1 cut(s)	411
EcoT38I	GRGCYC	2 cut(s)	1214, 1283
ErhI	CCWWGG	1 cut(s)	849
Esp3I	CGTCTC	2 cut(s)	148, 177
FauI	CCCGC	3 cut(s)	225, 1447, 1530
FauNDI	CATATG	1 cut(s)	1431
FbaI	TGATCA	1 cut(s)	202
Fnu4HI	GCNGC	5 cut(s)	334, 597, 1004, 1065, 1611
FokI	GGATG	7 cut(s)	440, 634, 1349, 1371, 1428, 1618, 1670
FriOI	GRGCYC	2 cut(s)	1214, 1283
Fsp4HI	GCNGC	5 cut(s)	334, 597, 1004, 1065, 1611
FspBI	CTAG	5 cut(s)	123, 129, 422, 972, 1614
GlaI	GCGC	1 cut(s)	1624
GluI	GCNGC	5 cut(s)	334, 597, 1004, 1065, 1611
HaeIII	GGCC	4 cut(s)	336, 1281, 1582, 1830
HapII	CCGG	7 cut(s)	375, 390, 794, 1263, 1473, 1552, 1831
HhaI	GCGC	1 cut(s)	1625
Hin1I	GRCGYC	2 cut(s)	68, 170
Hin6I	GCGC	1 cut(s)	1623
HinP1I	GCGC	1 cut(s)	1623
HincII	GTYRAC	1 cut(s)	96
HindII	GTYRAC	1 cut(s)	96
HindIII	AAGCTT	2 cut(s)	1490, 1791
HinfI	GANTC	4 cut(s)	563, 704, 1267, 1408
HpaI	GTTAAC	1 cut(s)	96
HpaII	CCGG	7 cut(s)	375, 390, 794, 1263, 1473, 1552, 1831
HphI	GGTGA	8 cut(s)	66, 247, 297, 312, 525, 699, 1146, 1936
Hpy166II	GTNNAC	1 cut(s)	96
Hpy188III	TCNNGA	8 cut(s)	81, 200, 977, 1070, 1207, 1473, 1714, 1907
Hpy8I	GTNNAC	1 cut(s)	96
Hpy99I	CGWCG	2 cut(s)	1113, 1442
HpyCH4III	ACNGT	4 cut(s)	303, 1084, 1204, 1648
HpyCH4IV	ACGT	3 cut(s)	68, 170, 2055
HpyCH4V	TGCA	9 cut(s)	9, 299, 409, 451, 584, 691, 752, 806, 1960
HpyF10VI	GCNNNNNNNGC	5 cut(s)	406, 581, 641, 697, 926
HpyF3I	CTNAG	7 cut(s)	264, 487, 716, 734, 1370, 1425, 1770
HpySE526I	ACGT	3 cut(s)	68, 170, 2055
Hsp92I	GRCGYC	2 cut(s)	68, 170
HspAI	GCGC	1 cut(s)	1623
Kpn2I	TCCGGA	1 cut(s)	1472
Ksp22I	TGATCA	1 cut(s)	202
KspAI	GTTAAC	1 cut(s)	96
Kzo9I	GATC	9 cut(s)	196, 202, 268, 466, 796, 958, 1316, 1441, 1978
LmnI	GCTCC	2 cut(s)	185, 572
Lsp1109I	GCAGC	4 cut(s)	608, 990, 1051, 1622
LweI	GCATC	2 cut(s)	418, 532
MaeI	CTAG	5 cut(s)	123, 129, 422, 972, 1614
MaeII	ACGT	3 cut(s)	68, 170, 2055
MaeIII	GTNAC	6 cut(s)	303, 660, 870, 1078, 1308, 1336
MalI	GATC	9 cut(s)	198, 204, 270, 468, 798, 960, 1318, 1443, 1980
MbiI	CCGCTC	1 cut(s)	232
MboI	GATC	9 cut(s)	196, 202, 268, 466, 796, 958, 1316, 1441, 1978
MfeI	CAATTG	1 cut(s)	1857
MflI	RGATCY	4 cut(s)	268, 796, 958, 1316
MhlI	GDGCHC	3 cut(s)	509, 1214, 1283
MlsI	TGGCCA	1 cut(s)	1582
MluNI	TGGCCA	1 cut(s)	1582
MlyI	GAGTC	1 cut(s)	698
MmeI	TCCRAC	4 cut(s)	79, 185, 538, 1630
Mox20I	TGGCCA	1 cut(s)	1582
Mph1103I	ATGCAT	1 cut(s)	411
MroI	TCCGGA	1 cut(s)	1472
MroXI	GAANNNNTTC	1 cut(s)	1326
MscI	TGGCCA	1 cut(s)	1582
MseI	TTAA	6 cut(s)	95, 114, 603, 1166, 1488, 2062
MslI	CAYNNNNRTG	4 cut(s)	216, 862, 915, 1205
Msp20I	TGGCCA	1 cut(s)	1582
MspA1I	CMGCKG	1 cut(s)	951
MspI	CCGG	7 cut(s)	375, 390, 794, 1263, 1473, 1552, 1831
MspR9I	CCNGG	5 cut(s)	375, 1264, 1527, 1806, 1827
MunI	CAATTG	1 cut(s)	1857
MvaI	CCWGG	3 cut(s)	1527, 1806, 1827
MvnI	CGCG	1 cut(s)	1625
MwoI	GCNNNNNNNGC	5 cut(s)	406, 581, 641, 697, 926
NciI	CCSGG	2 cut(s)	375, 1264
NcoI	CCATGG	1 cut(s)	849
NdeI	CATATG	1 cut(s)	1431
NdeII	GATC	9 cut(s)	196, 202, 268, 466, 796, 958, 1316, 1441, 1978
NlaIV	GGNNCC	5 cut(s)	337, 388, 960, 1281, 1421
NmuCI	GTSAC	2 cut(s)	303, 660
NsiI	ATGCAT	1 cut(s)	411
NspI	RCATGY	4 cut(s)	221, 579, 1309, 1454
PaeI	GCATGC	1 cut(s)	579
PagI	TCATGA	5 cut(s)	199, 976, 1069, 1206, 1906
PciI	ACATGT	2 cut(s)	217, 1305
PdmI	GAANNNNTTC	1 cut(s)	1326
PfeI	GAWTC	3 cut(s)	563, 1267, 1408
PkrI	GCNGC	5 cut(s)	335, 598, 1005, 1066, 1612
PleI	GAGTC	1 cut(s)	698
PpsI	GAGTC	1 cut(s)	698
PscI	ACATGT	2 cut(s)	217, 1305
PshAI	GACNNNNGTC	1 cut(s)	1041
PshBI	ATTAAT	1 cut(s)	114
PsiI	TTATAA	1 cut(s)	404
Psp6I	CCWGG	3 cut(s)	1525, 1804, 1825
PspGI	CCWGG	3 cut(s)	1525, 1804, 1825
PspN4I	GGNNCC	5 cut(s)	337, 388, 960, 1281, 1421
PspOMI	GGGCCC	1 cut(s)	1279
PspPI	GGNCC	5 cut(s)	87, 335, 986, 1279, 1280
PsrI	GAACNNNNNNTAC	2 cut(s)	463, 495
PstI	CTGCAG	1 cut(s)	693
PsuI	RGATCY	4 cut(s)	268, 796, 958, 1316
RsaI	GTAC	4 cut(s)	292, 1027, 1635, 1645
RsaNI	GTAC	4 cut(s)	291, 1026, 1634, 1644
RseI	CAYNNNNRTG	4 cut(s)	216, 862, 915, 1205
SaqAI	TTAA	6 cut(s)	95, 114, 603, 1166, 1488, 2062
SatI	GCNGC	5 cut(s)	334, 597, 1004, 1065, 1611
Sau3AI	GATC	9 cut(s)	196, 202, 268, 466, 796, 958, 1316, 1441, 1978
Sau96I	GGNCC	5 cut(s)	87, 335, 986, 1279, 1280
ScaI	AGTACT	1 cut(s)	292
SchI	GAGTC	1 cut(s)	698
ScrFI	CCNGG	5 cut(s)	375, 1264, 1527, 1806, 1827
SduI	GDGCHC	3 cut(s)	509, 1214, 1283
SfaNI	GCATC	2 cut(s)	418, 532
SfcI	CTRYAG	2 cut(s)	689, 1284
SinI	GGWCC	2 cut(s)	87, 986
SmiMI	CAYNNNNRTG	4 cut(s)	216, 862, 915, 1205
SmlI	CTYRAG	1 cut(s)	2037
SmoI	CTYRAG	1 cut(s)	2037
SphI	GCATGC	1 cut(s)	579
SsiI	CCGC	6 cut(s)	232, 333, 951, 1041, 1454, 1537
SspMI	CTAG	5 cut(s)	123, 129, 422, 972, 1614
StyD4I	CCNGG	5 cut(s)	373, 1262, 1525, 1804, 1825
StyI	CCWWGG	1 cut(s)	849
TaaI	ACNGT	4 cut(s)	303, 1084, 1204, 1648
TaiI	ACGT	3 cut(s)	71, 173, 2058
TaqI	TCGA	6 cut(s)	214, 435, 469, 615, 1437, 1680
TaqII	GACCGA	1 cut(s)	504
TatI	WGTACW	2 cut(s)	290, 1025
TauI	GCSGC	1 cut(s)	336
TfiI	GAWTC	3 cut(s)	563, 1267, 1408
Tru1I	TTAA	6 cut(s)	95, 114, 603, 1166, 1488, 2062
Tru9I	TTAA	6 cut(s)	95, 114, 603, 1166, 1488, 2062
TscAI	CASTG	1 cut(s)	1395
TseFI	GTSAC	2 cut(s)	303, 660
TseI	GCWGC	4 cut(s)	596, 1003, 1064, 1610
Tsp45I	GTSAC	2 cut(s)	303, 660
TspDTI	ATGAA	9 cut(s)	75, 685, 716, 993, 1415, 1701, 1712, 1743, 1923
TspGWI	ACGGA	1 cut(s)	1880
TspRI	CASTG	1 cut(s)	1395
VpaK11BI	GGWCC	2 cut(s)	87, 986
VspI	ATTAAT	1 cut(s)	114
XagI	CCTNNNNNAGG	2 cut(s)	655, 1371
XapI	RAATTY	3 cut(s)	1701, 1809, 1820
XceI	RCATGY	4 cut(s)	221, 579, 1309, 1454
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2021
XmnI	GAANNNNTTC	1 cut(s)	1326
XspI	CTAG	5 cut(s)	123, 129, 422, 972, 1614
ZraI	GACGTC	2 cut(s)	69, 171
ZrmI	AGTACT	1 cut(s)	292
Zsp2I	ATGCAT	1 cut(s)	411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.