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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 182 |
| AcsI | RAATTY | 7 cut(s) | 80, 94, 102, 114, 245, 323, 375 |
| AcuI | CTGAAG | 1 cut(s) | 201 |
| AfaI | GTAC | 1 cut(s) | 292 |
| AgsI | TTSAA | 4 cut(s) | 101, 112, 135, 244 |
| AluBI | AGCT | 2 cut(s) | 215, 273 |
| AluI | AGCT | 2 cut(s) | 215, 273 |
| Alw26I | GTCTC | 1 cut(s) | 302 |
| AlwI | GGATC | 1 cut(s) | 182 |
| ApoI | RAATTY | 7 cut(s) | 80, 94, 102, 114, 245, 323, 375 |
| AspA2I | CCTAGG | 1 cut(s) | 223 |
| AsuHPI | GGTGA | 2 cut(s) | 23, 364 |
| AsuII | TTCGAA | 1 cut(s) | 91 |
| AvrII | CCTAGG | 1 cut(s) | 223 |
| BccI | CCATC | 1 cut(s) | 2 |
| BcoDI | GTCTC | 1 cut(s) | 302 |
| BfaI | CTAG | 1 cut(s) | 224 |
| BlnI | CCTAGG | 1 cut(s) | 223 |
| Bpu14I | TTCGAA | 1 cut(s) | 91 |
| BsaI | GGTCTC | 1 cut(s) | 302 |
| BsaJI | CCNNGG | 1 cut(s) | 223 |
| BseDI | CCNNGG | 1 cut(s) | 223 |
| BseGI | GGATG | 1 cut(s) | 13 |
| BsmAI | GTCTC | 1 cut(s) | 302 |
| Bso31I | GGTCTC | 1 cut(s) | 302 |
| Bsp119I | TTCGAA | 1 cut(s) | 91 |
| Bsp1407I | TGTACA | 1 cut(s) | 290 |
| Bsp143I | GATC | 1 cut(s) | 187 |
| BspPI | GGATC | 1 cut(s) | 182 |
| BspT104I | TTCGAA | 1 cut(s) | 91 |
| BspTNI | GGTCTC | 1 cut(s) | 302 |
| BsrGI | TGTACA | 1 cut(s) | 290 |
| BssECI | CCNNGG | 1 cut(s) | 223 |
| BssMI | GATC | 1 cut(s) | 187 |
| BssT1I | CCWWGG | 1 cut(s) | 223 |
| BstAUI | TGTACA | 1 cut(s) | 290 |
| BstBI | TTCGAA | 1 cut(s) | 91 |
| BstC8I | GCNNGC | 1 cut(s) | 213 |
| BstDEI | CTNAG | 1 cut(s) | 166 |
| BstF5I | GGATG | 1 cut(s) | 13 |
| BstKTI | GATC | 1 cut(s) | 190 |
| BstMAI | GTCTC | 1 cut(s) | 302 |
| BstMBI | GATC | 1 cut(s) | 187 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 212 |
| BtsCI | GGATG | 1 cut(s) | 13 |
| Cac8I | GCNNGC | 1 cut(s) | 213 |
| Csp6I | GTAC | 1 cut(s) | 291 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 266, 301 |
| CviAII | CATG | 1 cut(s) | 85 |
| CviJI | RGCY | 3 cut(s) | 215, 273, 319 |
| CviKI_1 | RGCY | 3 cut(s) | 215, 273, 319 |
| CviQI | GTAC | 1 cut(s) | 291 |
| DdeI | CTNAG | 1 cut(s) | 166 |
| DpnI | GATC | 1 cut(s) | 189 |
| DpnII | GATC | 1 cut(s) | 187 |
| DraI | TTTAAA | 1 cut(s) | 79 |
| Eco130I | CCWWGG | 1 cut(s) | 223 |
| Eco31I | GGTCTC | 1 cut(s) | 302 |
| Eco57I | CTGAAG | 1 cut(s) | 201 |
| EcoT14I | CCWWGG | 1 cut(s) | 223 |
| ErhI | CCWWGG | 1 cut(s) | 223 |
| FaeI | CATG | 1 cut(s) | 88 |
| FaiI | YATR | 3 cut(s) | 52, 86, 160 |
| FatI | CATG | 1 cut(s) | 84 |
| FokI | GGATG | 1 cut(s) | 20 |
| FspBI | CTAG | 1 cut(s) | 224 |
| Hin1II | CATG | 1 cut(s) | 88 |
| HinfI | GANTC | 3 cut(s) | 17, 230, 257 |
| HphI | GGTGA | 2 cut(s) | 23, 364 |
| Hpy166II | GTNNAC | 3 cut(s) | 155, 291, 355 |
| Hpy188I | TCNGA | 7 cut(s) | 36, 251, 267, 283, 312, 336, 386 |
| Hpy8I | GTNNAC | 3 cut(s) | 155, 291, 355 |
| HpyAV | CCTTC | 1 cut(s) | 362 |
| HpyCH4V | TGCA | 2 cut(s) | 175, 206 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 212 |
| HpyF3I | CTNAG | 1 cut(s) | 166 |
| Hsp92II | CATG | 1 cut(s) | 88 |
| Kzo9I | GATC | 1 cut(s) | 187 |
| LmnI | GCTCC | 2 cut(s) | 251, 278 |
| LpnPI | CCDG | 2 cut(s) | 126, 279 |
| MaeI | CTAG | 1 cut(s) | 224 |
| MaeIII | GTNAC | 1 cut(s) | 124 |
| MalI | GATC | 1 cut(s) | 189 |
| MboI | GATC | 1 cut(s) | 187 |
| MboII | GAAGA | 1 cut(s) | 384 |
| MluCI | AATT | 8 cut(s) | 66, 80, 94, 102, 114, 245, 323, 375 |
| MnlI | CCTC | 5 cut(s) | 19, 173, 201, 323, 343 |
| MseI | TTAA | 2 cut(s) | 78, 321 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 212 |
| NdeII | GATC | 1 cut(s) | 187 |
| NlaIII | CATG | 1 cut(s) | 88 |
| NmuCI | GTSAC | 1 cut(s) | 124 |
| NspV | TTCGAA | 1 cut(s) | 91 |
| PfeI | GAWTC | 3 cut(s) | 17, 230, 257 |
| RsaI | GTAC | 1 cut(s) | 292 |
| RsaNI | GTAC | 1 cut(s) | 291 |
| SaqAI | TTAA | 2 cut(s) | 78, 321 |
| Sau3AI | GATC | 1 cut(s) | 187 |
| SetI | ASST | 7 cut(s) | 30, 165, 217, 275, 298, 334, 354 |
| SfuI | TTCGAA | 1 cut(s) | 91 |
| SgeI | CNNG | 9 cut(s) | 97, 131, 136, 153, 204, 224, 236, 250, 306 |
| Sse9I | AATT | 8 cut(s) | 66, 80, 94, 102, 114, 245, 323, 375 |
| SspMI | CTAG | 1 cut(s) | 224 |
| StyI | CCWWGG | 1 cut(s) | 223 |
| TaqI | TCGA | 2 cut(s) | 91, 186 |
| TasI | AATT | 8 cut(s) | 66, 80, 94, 102, 114, 245, 323, 375 |
| TatI | WGTACW | 1 cut(s) | 290 |
| TfiI | GAWTC | 3 cut(s) | 17, 230, 257 |
| Tru1I | TTAA | 2 cut(s) | 78, 321 |
| Tru9I | TTAA | 2 cut(s) | 78, 321 |
| TseFI | GTSAC | 1 cut(s) | 124 |
| Tsp45I | GTSAC | 1 cut(s) | 124 |
| TspDTI | ATGAA | 2 cut(s) | 73, 381 |
| XapI | RAATTY | 7 cut(s) | 80, 94, 102, 114, 245, 323, 375 |
| XmaJI | CCTAGG | 1 cut(s) | 223 |
| XspI | CTAG | 1 cut(s) | 224 |