Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0000740

Physical Location & Seq

Genomic Coordinates Forward (+)

408382 .. 413394

Gene Structure 5013 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00076650.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1878 bp

ATGGCCTCCAACGCACTCTCTCTCCTCCTCCTGCTGCCGCTCCCCTCCATTTTCCGACATGAACCGACGTCTTCACCATTACTATCCACAAGGTCCAAGCTACCTCCGCTGGCTAGGACCACCAGATTGGCCATTTCCACAGACCAAACTCCAGTCTTCACGACGTCAACGGCAACTGCTTTATCAGAGTCAGACAAGTCCAAGACCAAGGAAGAGCACTTGGCTCTGGAGGACGACGGTGATGATCAAGCATCGGAAGAGCGTGCGAGCTTAAACCAGTTCTTTGAGCAAGCCAAGGGTCTTCTCAGATCGGACGACCAGTACGGCACTGGAGCTGGACCACCTCGCTGGTTCTGGCCTTTAGACTGCGTGGGGAGCGGGACCCCTCCAACCATGATTCCCCGCTCCTTCTCTTTTTGCCTGGTCTCGTGCTTGCATATCCCTCCTAAGGATCGAACACCGTTTACAGACTTAGTGAAGCTGGTTGAGAGAACAATTCGTTCAGAGCATGATGCCTCACCCGATCGTCCCATATATCTTGTTGGAGAATCTCTTGGAGCATGCCTTGCACTATCTGTGGCAGCCCTTAACCCGGATATCGACCTTGAGCTTATTTTAGCAAATCCAGCAACATCCTTCAGCAAGTCACCACTTCAAGCTGTATTACCTTTACTGCAGTTCATGCCTGACTCTCCTGATCTTAGCCTTCCTCATATACTGAGTACAATTACAGGTGCAATGGTGGCTAGTCTGGAGAAGAGAGTTGGAGGACTTCCACTGCCACAGACAGTTGGAAAGCTATCACGGGATATTATTGAACCATCAATATCTAACCTTTCTGTTCTTGCTGATATCTTGCCCAGAGAAACACTTCTCTGGAAGCTACAAATGATCAGTACAGCTTCTTCATATGCCAATTCACGCCTTCATGCTGTAAAAGCTCATACCGTGATATTATCCAGTGGAAGAGATCCATTGCTACCTAGTCAAGAGGAAGGTCAAAGACTCAAAAGCCTGCTTGCAACTTGTCAGGAAGATGCTTTTGATTTGCTAACTGTCATCAAGAATGTTGGTATCTATCGTCGTTCAAAAGAACGAGACTTTGTTTCAGATTACATACCACCAACACCTTCCGAAGTTAAAGGCATAATGGAAAAAAACAGATGGGTTAACAAAATCATGAGCCCAGTGATGCTCTCAACTTTAGCAGACGGAAAGATAGTGAGGGGCTTTGCCGGGATTCCTACAGAGGGTCCTGTATTGTATGTTGGTTATCACATGTTACTGGGATTTGAAATAGTTCCCTTGGTGACCCAACTACTTATGGGCGCTGTTCCTGTCTCGGGAAAAAACTTTTTCAAACTTCTCTCTACAAAGTCTCATGTCCTACTCTATCCAGGTGGAGTGCGTGAGGCTCTTCACCGGAAGGGGGAAGCATATAAGTTATTCTGGCCGGAACGGTCAGAATTTGTGAGGATGGCAGCTAGATTTGGAGCCAAAATCGTACCTTTTGGTGCTGTTGGAGAAGATGATATTGGTGATCTACTTCTCGATTATGAAGACCAGATGAAAATTCCTTTCCTTAGGAAATCTATAGAGGACTTAACGGCTGAAGCTGCAAAAGTGAGGTCCACCGTTGATGGGGAGATTGGAAACCAAGCTATGCACCTTCCAGGAATTGTACCGAAATTTCCTGGCCGGTTTTATTATAAATTTGGGAAACCAATTGAAACAGCAGTAAAATCGGAGGTTGAGAAGTCCCTTGCTTATTTGCAGAAAAAAAGAGAAAGTGATCCTTACAGAAACCTCGCGGCTCGGTTACAATACCAGGCCATACATGGGTTTACGTCTGAAGTTCCGTCATTTGACCTTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010287 GO:0016101 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0019432 GO:0033306 GO:0034308 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:1901576 GO:1901615 GO:1903173

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6 DAGAT Hydrolase_4

Protein Analysis

625

Amino Acids

68.82

Weight (kDa)

6.5

Isoelectric Point (pI)

48.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase_4	PF12146	157 - 331	6.6e-06	Serine aminopeptidase, S33
DAGAT	PF03982	439 - 513	3.1e-09	Diacylglycerol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000740)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02030 AT3G02030 AT3G26820 AT3G26820 AT3G26840 AT5G41120 AT5G41120 AT5G41120 AT5G41130 AT5G41130 AT5G41130
fragaria_vesca	FvH4_3g35400 FvH4_3g39220 FvH4_3g39220 FvH4_3g39270 FvH4_3g39280 FvH4_3g39280 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060
malus_domestica	MD09G1005800.v1.1 MD17G1009900.v1.1
prunus_persica	Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1
pyrus_communis	pycom111g00420
rosa_chinensis	RchiOBHm_Chr2g0176241 RchiOBHm_Chr5g0070671 RchiOBHm_Chr5g0070681 RchiOBHm_Chr5g0070691
rosa_laevigata	RLG00000022394 RLG00000036149 RLG00000036150 RLG00000036152
rosa_multiflora	Rmu_co8466433.1_g000001 Rmu_sc0000249.1_g000001 Rmu_sc0000405.1_g000016 Rmu_sc0005197.1_g000009 Rmu_sc0005197.1_g000015 Rmu_sc0036323.1_g000001
rosa_roxburghii	Rroxscaffold_1G00010480 Rroxscaffold_1G00010530 Rroxscaffold_1G00010550 Rroxscaffold_1G00010560 Rroxscaffold_2G00076650 Rroxscaffold_5G00357250
rosa_rugosa	Rorug02G0592200 Rorug02G0592300 Rorug05G0407500 Rorug05G0407800
rosa_samantha	Rh2AG670700 Rh2BG682200 Rh2CG645000 Rh2DG695800 Rh5AG463200 Rh5AG463300 Rh5AG463400 Rh5BG481200 Rh5CG505400 Rh5CG505500 Rh5DG493300 Rh5DG493600 Rh5DG493700
rosa_wichuraiana	Rw2G055020 Rw5G043110 Rw5G043120 Rw5G043130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1710
AatII	GACGTC	2 cut(s)	71, 167
AccBSI	CCGCTC	3 cut(s)	40, 378, 405
AccII	CGCG	1 cut(s)	1811
AciI	CCGC	5 cut(s)	38, 107, 378, 403, 1811
AclWI	GGATC	3 cut(s)	459, 965, 1787
AcoI	YGGCCR	3 cut(s)	129, 1451, 1696
AcsI	RAATTY	4 cut(s)	1466, 1572, 1688, 1712
AcuI	CTGAAG	3 cut(s)	622, 1632, 1872
AcyI	GRCGYC	2 cut(s)	68, 164
AfaI	GTAC	5 cut(s)	323, 724, 898, 1506, 1683
AfiI	CCNNNNNNNGG	7 cut(s)	448, 592, 1250, 1343, 1429, 1641, 1839
AflIII	ACRYGT	1 cut(s)	1278
AgsI	TTSAA	6 cut(s)	656, 818, 1089, 1295, 1360, 1730
AjnI	CCWGG	5 cut(s)	420, 1396, 1672, 1693, 1827
AjuI	GAANNNNNNNTTGG	2 cut(s)	1518, 1550
Alw21I	GWGCWC	1 cut(s)	219
Alw26I	GTCTC	4 cut(s)	430, 1092, 1345, 1383
AlwI	GGATC	3 cut(s)	459, 965, 1787
Ama87I	CYCGRG	1 cut(s)	1342
AoxI	GGCC	6 cut(s)	3, 129, 356, 1451, 1696, 1830
ApeKI	GCWGC	4 cut(s)	34, 581, 1481, 1616
ApoI	RAATTY	4 cut(s)	1466, 1572, 1688, 1712
ArsI	GACNNNNNNTTYG	2 cut(s)	1613, 1645
Asp700I	GAANNNNTTC	2 cut(s)	870, 1299
AspLEI	GCGC	1 cut(s)	1331
AspS9I	GGNCC	6 cut(s)	93, 117, 338, 381, 1253, 1629
AsuC2I	CCSGG	2 cut(s)	593, 1237
AsuHPI	GGTGA	7 cut(s)	66, 251, 510, 639, 1321, 1412, 1550
AvaI	CYCGRG	1 cut(s)	1342
AvaII	GGWCC	6 cut(s)	93, 117, 338, 381, 1253, 1629
AxyI	CCTNAGG	2 cut(s)	447, 1583
BalI	TGGCCA	1 cut(s)	131
BanII	GRGCYC	1 cut(s)	1187
BarI	GAAGNNNNNNTAC	2 cut(s)	889, 921
BauI	CACGAG	1 cut(s)	427
BbsI	GAAGAC	4 cut(s)	63, 148, 293, 1566
Bbv12I	GWGCWC	1 cut(s)	219
BbvI	GCAGC	4 cut(s)	21, 593, 1493, 1603
BccI	CCATC	4 cut(s)	829, 1158, 1471, 1634
BceAI	ACGGC	3 cut(s)	186, 340, 1623
BcgI	CGANNNNNNTGC	2 cut(s)	1725, 1759
BciT130I	CCWGG	5 cut(s)	422, 1398, 1674, 1695, 1829
BclI	TGATCA	2 cut(s)	244, 891
BcnI	CCSGG	2 cut(s)	593, 1237
BcoDI	GTCTC	4 cut(s)	430, 1092, 1345, 1383
BfaI	CTAG	4 cut(s)	114, 747, 984, 1485
BfmI	CTRYAG	3 cut(s)	674, 1245, 1593
BfoI	RGCGCY	1 cut(s)	1332
BisI	GCNGC	6 cut(s)	35, 38, 582, 1482, 1617, 1812
BlsI	GCNGC	6 cut(s)	36, 39, 583, 1483, 1618, 1813
Bme1390I	CCNGG	7 cut(s)	422, 593, 1237, 1398, 1674, 1695, 1829
Bme18I	GGWCC	6 cut(s)	93, 117, 338, 381, 1253, 1629
BmeT110I	CYCGRG	1 cut(s)	1342
BmgT120I	GGNCC	6 cut(s)	93, 117, 338, 381, 1253, 1629
BmiI	GGNNCC	4 cut(s)	382, 383, 1254, 1495
BmrFI	CCNGG	7 cut(s)	422, 593, 1237, 1398, 1674, 1695, 1829
BmrI	ACTGGG	2 cut(s)	1181, 1295
BmsI	GCATC	4 cut(s)	260, 502, 1027, 1182
BmuI	ACTGGG	2 cut(s)	1181, 1295
BpiI	GAAGAC	4 cut(s)	63, 148, 293, 1566
BpmI	CTGGAG	4 cut(s)	135, 248, 351, 773
BpuEI	CTTGAG	1 cut(s)	626
BpuMI	CCSGG	2 cut(s)	593, 1237
BsaHI	GRCGYC	2 cut(s)	68, 164
BsaI	GGTCTC	1 cut(s)	430
BsaJI	CCNNGG	3 cut(s)	207, 294, 1305
BsaWI	WCCGGW	1 cut(s)	1422
BsaXI	ACNNNNNCTCC	2 cut(s)	6, 36
Bsc4I	CCNNNNNNNGG	7 cut(s)	448, 592, 1250, 1343, 1429, 1641, 1839
Bse118I	RCCGGY	1 cut(s)	1698
Bse1I	ACTGG	7 cut(s)	152, 277, 319, 334, 960, 1187, 1290
Bse21I	CCTNAGG	2 cut(s)	447, 1583
Bse3DI	GCAATG	2 cut(s)	744, 974
BseBI	CCWGG	5 cut(s)	422, 1398, 1674, 1695, 1829
BseDI	CCNNGG	3 cut(s)	207, 294, 1305
BseGI	GGATG	2 cut(s)	632, 1482
BseLI	CCNNNNNNNGG	7 cut(s)	448, 592, 1250, 1343, 1429, 1641, 1839
BseMI	GCAATG	2 cut(s)	744, 974
BseMII	CTCAG	2 cut(s)	319, 710
BseNI	ACTGG	7 cut(s)	152, 277, 319, 334, 960, 1187, 1290
BseRI	GAGGAG	2 cut(s)	14, 17
BseXI	GCAGC	4 cut(s)	21, 593, 1493, 1603
Bsh1236I	CGCG	1 cut(s)	1811
Bsh1285I	CGRYCG	1 cut(s)	526
BshFI	GGCC	6 cut(s)	5, 131, 358, 1453, 1698, 1832
BsiEI	CGRYCG	1 cut(s)	526
BsiHKAI	GWGCWC	1 cut(s)	219
BsiHKCI	CYCGRG	1 cut(s)	1342
BsiSI	CCGG	5 cut(s)	593, 1236, 1423, 1454, 1699
BslFI	GGGAC	3 cut(s)	394, 513, 1744
BslI	CCNNNNNNNGG	7 cut(s)	448, 592, 1250, 1343, 1429, 1641, 1839
BsmAI	GTCTC	4 cut(s)	430, 1092, 1345, 1383
BsmFI	GGGAC	3 cut(s)	394, 513, 1744
BsnI	GGCC	6 cut(s)	5, 131, 358, 1453, 1698, 1832
Bso31I	GGTCTC	1 cut(s)	430
BsoBI	CYCGRG	1 cut(s)	1342
Bsp1286I	GDGCHC	2 cut(s)	219, 1187
Bsp143I	GATC	9 cut(s)	244, 308, 451, 523, 697, 891, 970, 1540, 1792
BspACI	CCGC	5 cut(s)	38, 107, 378, 403, 1811
BspANI	GGCC	6 cut(s)	5, 131, 358, 1453, 1698, 1832
BspCNI	CTCAG	2 cut(s)	318, 711
BspFNI	CGCG	1 cut(s)	1811
BspHI	TCATGA	1 cut(s)	1179
BspLI	GGNNCC	4 cut(s)	382, 383, 1254, 1495
BspMAI	CTGCAG	1 cut(s)	678
BspPI	GGATC	3 cut(s)	459, 965, 1787
BspQI	GCTCTTC	3 cut(s)	207, 252, 1422
BspTNI	GGTCTC	1 cut(s)	430
BsrBI	CCGCTC	3 cut(s)	40, 378, 405
BsrDI	GCAATG	2 cut(s)	744, 974
BsrFI	RCCGGY	1 cut(s)	1698
BsrI	ACTGG	7 cut(s)	152, 277, 319, 334, 960, 1187, 1290
BssAI	RCCGGY	1 cut(s)	1698
BssECI	CCNNGG	3 cut(s)	207, 294, 1305
BssMI	GATC	9 cut(s)	244, 308, 451, 523, 697, 891, 970, 1540, 1792
BssNI	GRCGYC	2 cut(s)	68, 164
BssSI	CACGAG	1 cut(s)	427
BssT1I	CCWWGG	3 cut(s)	207, 294, 1305
Bst2BI	CACGAG	1 cut(s)	427
Bst2UI	CCWGG	5 cut(s)	422, 1398, 1674, 1695, 1829
Bst4CI	ACNGT	7 cut(s)	239, 462, 790, 949, 1057, 1461, 1636
Bst6I	CTCTTC	5 cut(s)	207, 252, 752, 961, 1422
BstACI	GRCGYC	2 cut(s)	68, 164
BstAPI	GCANNNNNTGC	2 cut(s)	566, 682
BstC8I	GCNNGC	8 cut(s)	111, 264, 268, 291, 434, 562, 1016, 1020
BstDEI	CTNAG	6 cut(s)	305, 447, 472, 701, 719, 1583
BstEII	GGTNACC	1 cut(s)	1309
BstF5I	GGATG	2 cut(s)	632, 1482
BstFNI	CGCG	1 cut(s)	1811
BstH2I	RGCGCY	1 cut(s)	1332
BstHHI	GCGC	1 cut(s)	1331
BstKTI	GATC	9 cut(s)	247, 311, 454, 526, 700, 894, 973, 1543, 1795
BstMAI	GTCTC	4 cut(s)	430, 1092, 1345, 1383
BstMBI	GATC	9 cut(s)	244, 308, 451, 523, 697, 891, 970, 1540, 1792
BstMCI	CGRYCG	1 cut(s)	526
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 106, 375, 566, 626, 682, 743, 938, 1616
BstNI	CCWGG	5 cut(s)	422, 1398, 1674, 1695, 1829
BstNSI	RCATGY	2 cut(s)	564, 1282
BstPI	GGTNACC	1 cut(s)	1309
BstSCI	CCNGG	7 cut(s)	420, 591, 1235, 1396, 1672, 1693, 1827
BstSFI	CTRYAG	3 cut(s)	674, 1245, 1593
BstUI	CGCG	1 cut(s)	1811
BstV1I	GCAGC	4 cut(s)	21, 593, 1493, 1603
BstV2I	GAAGAC	4 cut(s)	63, 148, 293, 1566
BstX2I	RGATCY	1 cut(s)	970
BstXI	CCANNNNNNTGG	2 cut(s)	127, 348
BstYI	RGATCY	1 cut(s)	970
Bsu36I	CCTNAGG	2 cut(s)	447, 1583
BsuRI	GGCC	6 cut(s)	5, 131, 358, 1453, 1698, 1832
BtsCI	GGATG	2 cut(s)	632, 1482
BtsI	GCAGTG	1 cut(s)	776
BtsIMutI	CAGTG	4 cut(s)	327, 776, 967, 1194
Cac8I	GCNNGC	8 cut(s)	111, 264, 268, 291, 434, 562, 1016, 1020
CciI	TCATGA	1 cut(s)	1179
CfoI	GCGC	1 cut(s)	1331
Cfr10I	RCCGGY	1 cut(s)	1698
Cfr13I	GGNCC	6 cut(s)	93, 117, 338, 381, 1253, 1629
Csp6I	GTAC	5 cut(s)	322, 723, 897, 1505, 1682
CviQI	GTAC	5 cut(s)	322, 723, 897, 1505, 1682
DdeI	CTNAG	6 cut(s)	305, 447, 472, 701, 719, 1583
DpnI	GATC	9 cut(s)	246, 310, 453, 525, 699, 893, 972, 1542, 1794
DpnII	GATC	9 cut(s)	244, 308, 451, 523, 697, 891, 970, 1540, 1792
EaeI	YGGCCR	3 cut(s)	129, 1451, 1696
Eam1104I	CTCTTC	5 cut(s)	207, 252, 752, 961, 1422
EarI	CTCTTC	5 cut(s)	207, 252, 752, 961, 1422
Eco130I	CCWWGG	3 cut(s)	207, 294, 1305
Eco24I	GRGCYC	1 cut(s)	1187
Eco31I	GGTCTC	1 cut(s)	430
Eco32I	GATATC	2 cut(s)	598, 853
Eco47I	GGWCC	6 cut(s)	93, 117, 338, 381, 1253, 1629
Eco57I	CTGAAG	3 cut(s)	622, 1632, 1872
Eco81I	CCTNAGG	2 cut(s)	447, 1583
Eco88I	CYCGRG	1 cut(s)	1342
Eco91I	GGTNACC	1 cut(s)	1309
EcoO109I	RGGNCCY	2 cut(s)	381, 1253
EcoO65I	GGTNACC	1 cut(s)	1309
EcoRII	CCWGG	5 cut(s)	420, 1396, 1672, 1693, 1827
EcoRV	GATATC	2 cut(s)	598, 853
EcoT14I	CCWWGG	3 cut(s)	207, 294, 1305
EcoT38I	GRGCYC	1 cut(s)	1187
ErhI	CCWWGG	3 cut(s)	207, 294, 1305
FalI	AAGNNNNNCTT	2 cut(s)	1780, 1812
FaqI	GGGAC	3 cut(s)	394, 513, 1744
FauI	CCCGC	2 cut(s)	371, 410
FauNDI	CATATG	1 cut(s)	910
FbaI	TGATCA	2 cut(s)	244, 891
Fnu4HI	GCNGC	6 cut(s)	35, 38, 582, 1482, 1617, 1812
FokI	GGATG	2 cut(s)	619, 1489
FriOI	GRGCYC	1 cut(s)	1187
Fsp4HI	GCNGC	6 cut(s)	35, 38, 582, 1482, 1617, 1812
FspBI	CTAG	4 cut(s)	114, 747, 984, 1485
GlaI	GCGC	1 cut(s)	1330
GluI	GCNGC	6 cut(s)	35, 38, 582, 1482, 1617, 1812
GsuI	CTGGAG	4 cut(s)	135, 248, 351, 773
HaeII	RGCGCY	1 cut(s)	1332
HaeIII	GGCC	6 cut(s)	5, 131, 358, 1453, 1698, 1832
HapII	CCGG	5 cut(s)	593, 1236, 1423, 1454, 1699
HhaI	GCGC	1 cut(s)	1331
Hin1I	GRCGYC	2 cut(s)	68, 164
Hin6I	GCGC	1 cut(s)	1329
HinP1I	GCGC	1 cut(s)	1329
HincII	GTYRAC	2 cut(s)	168, 1171
HindII	GTYRAC	2 cut(s)	168, 1171
HinfI	GANTC	6 cut(s)	188, 397, 548, 689, 1005, 1240
HpaI	GTTAAC	1 cut(s)	1171
HpaII	CCGG	5 cut(s)	593, 1236, 1423, 1454, 1699
HphI	GGTGA	7 cut(s)	66, 251, 510, 639, 1321, 1412, 1550
Hpy166II	GTNNAC	5 cut(s)	168, 465, 1171, 1632, 1845
Hpy8I	GTNNAC	5 cut(s)	168, 465, 1171, 1632, 1845
Hpy99I	CGWCG	4 cut(s)	70, 166, 239, 1086
HpyAV	CCTTC	8 cut(s)	418, 646, 716, 935, 989, 1140, 1420, 1679
HpyCH4III	ACNGT	7 cut(s)	239, 462, 790, 949, 1057, 1461, 1636
HpyCH4IV	ACGT	3 cut(s)	68, 164, 1847
HpyCH4V	TGCA	8 cut(s)	436, 569, 676, 737, 1022, 1619, 1666, 1774
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 106, 375, 566, 626, 682, 743, 938, 1616
HpyF3I	CTNAG	6 cut(s)	305, 447, 472, 701, 719, 1583
HpySE526I	ACGT	3 cut(s)	68, 164, 1847
Hsp92I	GRCGYC	2 cut(s)	68, 164
HspAI	GCGC	1 cut(s)	1329
KflI	GGGWCCC	1 cut(s)	381
Ksp22I	TGATCA	2 cut(s)	244, 891
KspAI	GTTAAC	1 cut(s)	1171
Kzo9I	GATC	9 cut(s)	244, 308, 451, 523, 697, 891, 970, 1540, 1792
LguI	GCTCTTC	3 cut(s)	207, 252, 1422
LmnI	GCTCC	6 cut(s)	45, 332, 375, 410, 557, 1493
Lsp1109I	GCAGC	4 cut(s)	21, 593, 1493, 1603
LweI	GCATC	4 cut(s)	260, 502, 1027, 1182
MaeI	CTAG	4 cut(s)	114, 747, 984, 1485
MaeII	ACGT	3 cut(s)	68, 164, 1847
MaeIII	GTNAC	4 cut(s)	645, 1281, 1309, 1818
MalI	GATC	9 cut(s)	246, 310, 453, 525, 699, 893, 972, 1542, 1794
MbiI	CCGCTC	3 cut(s)	40, 378, 405
MboI	GATC	9 cut(s)	244, 308, 451, 523, 697, 891, 970, 1540, 1792
MfeI	CAATTG	1 cut(s)	1725
MflI	RGATCY	1 cut(s)	970
MhlI	GDGCHC	2 cut(s)	219, 1187
MlsI	TGGCCA	1 cut(s)	131
MluCI	AATT	9 cut(s)	495, 726, 916, 1466, 1572, 1677, 1688, 1712, 1725
MluNI	TGGCCA	1 cut(s)	131
MlyI	GAGTC	3 cut(s)	197, 683, 999
MmeI	TCCRAC	7 cut(s)	33, 79, 413, 523, 745, 772, 1501
Mox20I	TGGCCA	1 cut(s)	131
MroXI	GAANNNNTTC	2 cut(s)	870, 1299
MscI	TGGCCA	1 cut(s)	131
MseI	TTAA	6 cut(s)	272, 588, 1140, 1170, 1604, 1876
Msp20I	TGGCCA	1 cut(s)	131
MspA1I	CMGCKG	1 cut(s)	109
MspI	CCGG	5 cut(s)	593, 1236, 1423, 1454, 1699
MspR9I	CCNGG	7 cut(s)	422, 593, 1237, 1398, 1674, 1695, 1829
MunI	CAATTG	1 cut(s)	1725
MvaI	CCWGG	5 cut(s)	422, 1398, 1674, 1695, 1829
MvnI	CGCG	1 cut(s)	1811
MwoI	GCNNNNNNNGC	9 cut(s)	11, 106, 375, 566, 626, 682, 743, 938, 1616
NciI	CCSGG	2 cut(s)	593, 1237
NdeI	CATATG	1 cut(s)	910
NdeII	GATC	9 cut(s)	244, 308, 451, 523, 697, 891, 970, 1540, 1792
NlaIV	GGNNCC	4 cut(s)	382, 383, 1254, 1495
NmuCI	GTSAC	2 cut(s)	645, 1309
NspI	RCATGY	2 cut(s)	564, 1282
PaeI	GCATGC	1 cut(s)	564
PagI	TCATGA	1 cut(s)	1179
PciI	ACATGT	1 cut(s)	1278
PciSI	GCTCTTC	3 cut(s)	207, 252, 1422
PdmI	GAANNNNTTC	2 cut(s)	870, 1299
PfeI	GAWTC	3 cut(s)	397, 548, 1240
PfoI	TCCNGGA	1 cut(s)	1672
PkrI	GCNGC	6 cut(s)	36, 39, 583, 1483, 1618, 1813
Ple19I	CGATCG	1 cut(s)	526
PleI	GAGTC	3 cut(s)	196, 683, 999
PpsI	GAGTC	3 cut(s)	196, 683, 999
PpuMI	RGGWCCY	2 cut(s)	381, 1253
PscI	ACATGT	1 cut(s)	1278
PsiI	TTATAA	1 cut(s)	1710
Psp5II	RGGWCCY	2 cut(s)	381, 1253
Psp6I	CCWGG	5 cut(s)	420, 1396, 1672, 1693, 1827
PspEI	GGTNACC	1 cut(s)	1309
PspGI	CCWGG	5 cut(s)	420, 1396, 1672, 1693, 1827
PspN4I	GGNNCC	4 cut(s)	382, 383, 1254, 1495
PspPI	GGNCC	6 cut(s)	93, 117, 338, 381, 1253, 1629
PspPPI	RGGWCCY	2 cut(s)	381, 1253
PsrI	GAACNNNNNNTAC	2 cut(s)	448, 480
PstI	CTGCAG	1 cut(s)	678
PsuI	RGATCY	1 cut(s)	970
PvuI	CGATCG	1 cut(s)	526
RsaI	GTAC	5 cut(s)	323, 724, 898, 1506, 1683
RsaNI	GTAC	5 cut(s)	322, 723, 897, 1505, 1682
SapI	GCTCTTC	3 cut(s)	207, 252, 1422
SaqAI	TTAA	6 cut(s)	272, 588, 1140, 1170, 1604, 1876
SatI	GCNGC	6 cut(s)	35, 38, 582, 1482, 1617, 1812
Sau3AI	GATC	9 cut(s)	244, 308, 451, 523, 697, 891, 970, 1540, 1792
Sau96I	GGNCC	6 cut(s)	93, 117, 338, 381, 1253, 1629
SchI	GAGTC	3 cut(s)	197, 683, 999
ScrFI	CCNGG	7 cut(s)	422, 593, 1237, 1398, 1674, 1695, 1829
SduI	GDGCHC	2 cut(s)	219, 1187
SfaNI	GCATC	4 cut(s)	260, 502, 1027, 1182
SfcI	CTRYAG	3 cut(s)	674, 1245, 1593
SinI	GGWCC	6 cut(s)	93, 117, 338, 381, 1253, 1629
SmlI	CTYRAG	1 cut(s)	605
SmoI	CTYRAG	1 cut(s)	605
SphI	GCATGC	1 cut(s)	564
Sse9I	AATT	9 cut(s)	495, 726, 916, 1466, 1572, 1677, 1688, 1712, 1725
SsiI	CCGC	5 cut(s)	38, 107, 378, 403, 1811
SspMI	CTAG	4 cut(s)	114, 747, 984, 1485
StyD4I	CCNGG	7 cut(s)	420, 591, 1235, 1396, 1672, 1693, 1827
StyI	CCWWGG	3 cut(s)	207, 294, 1305
TaaI	ACNGT	7 cut(s)	239, 462, 790, 949, 1057, 1461, 1636
TaiI	ACGT	3 cut(s)	71, 167, 1850
TaqI	TCGA	3 cut(s)	454, 600, 1551
TasI	AATT	9 cut(s)	495, 726, 916, 1466, 1572, 1677, 1688, 1712, 1725
TatI	WGTACW	2 cut(s)	722, 896
TauI	GCSGC	2 cut(s)	40, 1814
TfiI	GAWTC	3 cut(s)	397, 548, 1240
Tru1I	TTAA	6 cut(s)	272, 588, 1140, 1170, 1604, 1876
Tru9I	TTAA	6 cut(s)	272, 588, 1140, 1170, 1604, 1876
TscAI	CASTG	4 cut(s)	334, 783, 967, 1194
TseFI	GTSAC	2 cut(s)	645, 1309
TseI	GCWGC	4 cut(s)	34, 581, 1481, 1616
Tsp45I	GTSAC	2 cut(s)	645, 1309
TspDTI	ATGAA	6 cut(s)	75, 670, 897, 917, 1572, 1583
TspGWI	ACGGA	2 cut(s)	1227, 1848
TspRI	CASTG	4 cut(s)	334, 783, 967, 1194
VpaK11BI	GGWCC	6 cut(s)	93, 117, 338, 381, 1253, 1629
XapI	RAATTY	4 cut(s)	1466, 1572, 1688, 1712
XceI	RCATGY	2 cut(s)	564, 1282
XcmI	CCANNNNNNNNNTGG	2 cut(s)	326, 1835
XmnI	GAANNNNTTC	2 cut(s)	870, 1299
XspI	CTAG	4 cut(s)	114, 747, 984, 1485
ZraI	GACGTC	2 cut(s)	69, 165

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.