Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0000740

Physical Location & Seq

Genomic Coordinates Reverse (-)

76498353 .. 76548412

Gene Structure 50060 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG481200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3846 bp

ATGTCCTGCAACGCACTTTGTCTCTTTCCCTGCCTCTTTCCTCCTTCCATTTTCCGACATGAATTGACGTCTTCACCATCAGGAAGGTCTAAGTTAACTGCCTTGTTCTCTATTAATCCATCTAGGACTAGGACTAGGAGGTTAGCCGTCTCCACACACCACACTCCAACCTTGACGTCTCGGATAACGGGAGCTATGGAGGATCATGATCATCGTCATCGACATGTAGAGGACGAGCGGGTGAGCTTCAAACATTTTATTGAGCAAGCTAAGGATCTTGTCAGACCAGACAGCAGTACTTCTGCACCGTCACCTCGCTGGTTCTCACCTTTAGATTGCGGCCCCCCATTAGACAATTCCCCCCTGCTTCTCTTTTTGCCCGGAATTGATGGCACCGGACTTGGGCTTATAATGCATCACAAGAAACTAGGAAAGATTTTCGAGGTATGGTGCTTGCACATCCCTCCTAAAGATCGAACACCATTTACAGACTTAGTGAAGCTAATTGAGAGCACAATTCGGTTAGAGCATCATCGCTCACCTAATAGACCAATATATCTTGTTGGAGAATCGCTTGGAGCATGCCTTGCACTATCTGTGGCAGCCTTTAACCCTGACATCGACCTTGTTCTAATTTTAGCAAATCCAGCAACATCCTTCAGCAGGTCACAACTTCAACCCATATTACATTTACTGCAGTTCATGCCTGACTCACCCAATCTTAGCCTTCTTCATATACTCAGTTCAATGACTGGTGCAATGATGGATAATTTGGAGAAGGGAATTGAAAGGCTATCGCCGGATCTTGTTGCACCATCAATAGCTTACCTCTCTGTTATTGCTGATATCTTACCCATGGAAACACTTGTATGGAAGCTACAAATGATTAGTTCAGCTTCTTCATATGCCAATTCACACCTCCATGCTGTGAAGGCTCAAACCCTGATACTTTCCAGCGGAAGGGATCCATTGCTACCTAGTCATGAAGAAGGTCCGAGACTACGATGGCTGCTGGCAAATTGTGAAATCTGTACTTTTGAAGACTGCGGTCATTTCCTCTTCTTGGAAGCTGCTCATGATTTGGTAACTGTCATCAAGCAGGTTGGTGTCTATCGTCGTTCAAAAGAAAGAGACTTTGTTTCAGATTTCATACCACTATCACCTTCTGAATTAAAAAATGTATTGGACAAACACAGATGGATTAGCACAGTCATGAGCCCAGTTATACTCTCAACTTTAGAAGATGGGAATATAGTGAGGGGCTTTGCCGGGATTCCTTTAGAGGGCCCTGTAGTGTATGTTGGTTATCACATGTTACTGGGATCTGAACTCTTTCCATTGATAACTCTGTTTTCGGAACAAAACAACATCCCTCTGAGAGGTTTAGCACATCCAATGATGTTTATGAAATTGAATCAAGGATGGTTTCCTGAGCATATGTTCGACGATCTCAAACATGCGGGGGCTGTTCCTGTCTCCGGAAAAAACTTTTTTAAGCTTCTCTCTACAAAGTCTCATTTCCTACTCTTTCCTGGTGGATTGCGGGAGGCTTTACACCGCAAGGGGGAAGCATATAAGTTATTCTGGCCGGAAAGGGCAGAGTTTGTGAGGATGGCAGCTAGATTTGGCGCGAAGATTGTACCTTTCGGTACAGTTGGAGAGGATGATTTTGCTGACATATTATTCGATTATGAAGACCAGATGAAAATTCCATTCTTCAAGAAATATATAGATGACTTATCGGATAAAGTAGCTGGAAAAGTGAGGGCAACTTCTGAGGGGGAGGTAGGAAACCAAGCTTTACACTTGCCAGGAATTCTACCAAAATTTCCTGGCCGGTTTTATCATCATTTTGGGAAACCAATTGAAACGGAAGGGAGGAAGGAGGAATTACGAGACAAGGAAAATGCTCATGAATTGTATTTACAGGTGAAGTTAGAGGTTGAGAACTGCCTTGCTTATTTGCAGGAAAAAAGAGAGAGTGATCCATACAGAAGCCTTGTGTCTCGGATACTATACCAAGCCAAAAATGGCTTTACAGCTCAACTATTTTGCCTCTCACGAATTACACACCAAAAACGAAGCCAGACAAGGAGAGAGCCTAGAACACTAGTTCATGGCCTCCAACGCCCTTCCTCTCTTCCCCTGCCGCTTTCCGACCATTCTCCGACGATGACTAGAAGGATAGCCGTCTCCACACGCCACACCATGTCTGACATGACCAGAACCGGAGAGAACCAGAGCCAGCCTTTGAAGGAAGAGCGTGTGAGCTTAAAGCATTTTTTCCAGCAGGCCAAGGATCTCGTCTCATCGGACATCGGACCACCTCACTGGTTCTCGCCTTTAGACTGCGGTCACCCCTTACATGATTCCCCCCTGCTTATCTTTTTGCCGGGAATTGATGGCATTGGACTTGGGCTTATAATGCATCATAAGAAACTTGGAAAGATTTTTGAGGTCTTGTGCTTGCATATTCCTCCCAAGGATCAAACACCAATTACAGACCTAGTGAAGCTAGTTGAGAGAAAAATTCGGTCAGATCATCATTTCTCACCTAATAGACCAATATATATTGTTGGAGAATCTGTTGGAGCATGCCTTGCACTATCTGTGGCATCCCTTAACCCTGATATCGACCTTGTTCTACTTTTAGCAAATCCTGGTAACATATATTTGTCATCACTTCAACCCATATTACCTTTACTGCAGTTCATGTCTGAGTTATTACCCAATCTTAGTCTTCCTCATGTACTGAGTTCAATGGCAGGTGCGATGATGGCTGATTTAGAGAAGGGCGTTGAAAGGCTAACGCGGGATCTTGTTGCACCATCAATAACTTACCTCTCTGTTCTTGCTGATATCTTACCCATAGAAACACTTCTGTGGAAGCTACAAATAATTAATTCAGCTTCTTCGTATGCCAATTCACGCCTCCATGCTGTAAAAGCTCAAACCCTGATACTTTCCAGTGGAAGAGATCCATTGCTACATAATCAAGAAGAAGGTCTGAGACTCCAACGGCTGCTTGCAAATTGTGAGATCCGTACATTTGAAGACTATGGCCATTTCCTCTTCTTGCAGGTTGGTGTCTATCGTCGTTTAAAAGAACGAGATTTTGTTCTAGATTACATACCACCATCACCTTCTGAATTAAAAGATGTACTGGACAAATACAGATGGATTAGCATAATTATGAGCCCAGTGATGCTCTCAACTTTAGCAGATGGGAAGATAGTGAGGGGCTTTGCTGGGATTCCATCAGAGGGTCCTGTAGTGTATGTCGGTTATCACATGTTACTGGGATTTGATATCTTTCCATTGGTAGCACAGTTGTGGGAACAAAAAGGCATCCATCTGCGAGGGATAGCACATCCAATGATGTTTATGAAATTGAAACAAGTAAATTTAGTTGAGTTACCCTTTTCCGACAATTTCAGACTGATGGGTGCTGTTCCTGTCTCAGGAAAAAACTTTTTCAAGCTACTCTCTATAAAGTCTCATATCCTACTCTATCCTGGTGGAGTGCGTGAGGCTCTTCACAGGAAGGGGGAAGCATATAAGCTATTTTGGCCTGAACAGTCAGAGTTTGTGAGGATGGCAGCTAGATTTGGAGCCAAAATCATACCCTTTGGTGCTGTTGGAGAAGATGACTGGGGTGACGTATTATTCGATTATGAAGATCAGATGAAAGCTCCTTTCCTCAAGAAATATGTAGAAGAAAAATCAGATGAAGCTGGAAAACTGAGAAAAAATGAGAGTGATCCTTACAGAAGCCTTGCATCTCGGCTACTCTTCCAGGCAAGACATGGCTTTACAGCTCAAGTTCCTGCATTTGACATCGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010287 GO:0016101 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0017144 GO:0019432 GO:0033306 GO:0034308 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:1901576 GO:1901615 GO:1903173

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6 DAGAT Hydrolase_4

Protein Analysis

1281

Amino Acids

144.41

Weight (kDa)

7.89

Isoelectric Point (pI)

50.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_6	PF12697	156 - 362	3.4e-08	Alpha/beta hydrolase family
Abhydrolase_1	PF00561	159 - 357	2.6e-07	alpha/beta hydrolase fold
Hydrolase_4	PF12146	162 - 356	1.6e-06	Serine aminopeptidase, S33
DAGAT	PF03982	486 - 558	1.5e-06	Diacylglycerol acyltransferase
Hydrolase_4	PF12146	834 - 1025	2.5e-06	Serine aminopeptidase, S33
DAGAT	PF03982	1146 - 1217	4.6e-06	Diacylglycerol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000740)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02030 AT3G02030 AT3G26820 AT3G26820 AT3G26840 AT5G41120 AT5G41120 AT5G41120 AT5G41130 AT5G41130 AT5G41130
fragaria_vesca	FvH4_3g35400 FvH4_3g39220 FvH4_3g39220 FvH4_3g39270 FvH4_3g39280 FvH4_3g39280 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060 FvH4_6g54060
malus_domestica	MD09G1005800.v1.1 MD17G1009900.v1.1
prunus_persica	Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1 Prupe.3G311200_v2.0.a1
pyrus_communis	pycom111g00420
rosa_chinensis	RchiOBHm_Chr2g0176241 RchiOBHm_Chr5g0070671 RchiOBHm_Chr5g0070681 RchiOBHm_Chr5g0070691
rosa_laevigata	RLG00000022394 RLG00000036149 RLG00000036150 RLG00000036152
rosa_multiflora	Rmu_co8466433.1_g000001 Rmu_sc0000249.1_g000001 Rmu_sc0000405.1_g000016 Rmu_sc0005197.1_g000009 Rmu_sc0005197.1_g000015 Rmu_sc0036323.1_g000001
rosa_roxburghii	Rroxscaffold_1G00010480 Rroxscaffold_1G00010530 Rroxscaffold_1G00010550 Rroxscaffold_1G00010560 Rroxscaffold_2G00076650 Rroxscaffold_5G00357250
rosa_rugosa	Rorug02G0592200 Rorug02G0592300 Rorug05G0407500 Rorug05G0407800
rosa_samantha	Rh2AG670700 Rh2BG682200 Rh2CG645000 Rh2DG695800 Rh5AG463200 Rh5AG463300 Rh5AG463400 Rh5BG481200 Rh5CG505400 Rh5CG505500 Rh5DG493300 Rh5DG493600 Rh5DG493700
rosa_wichuraiana	Rw2G055020 Rw5G043110 Rw5G043120 Rw5G043130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	410, 2423
AarI	CACCTGC	1 cut(s)	2756
AatII	GACGTC	2 cut(s)	71, 179
Acc36I	ACCTGC	4 cut(s)	654, 1090, 2756, 3070
AccB1I	GGYRCC	1 cut(s)	392
AccBSI	CCGCTC	1 cut(s)	238
AccII	CGCG	2 cut(s)	1631, 2812
AccIII	TCCGGA	1 cut(s)	1478
AcoI	YGGCCR	3 cut(s)	1586, 1834, 3061
AcsI	RAATTY	5 cut(s)	1707, 1815, 1826, 2529, 3403
AcuI	CTGAAG	1 cut(s)	643
AcyI	GRCGYC	2 cut(s)	68, 176
AfaI	GTAC	7 cut(s)	298, 1033, 1641, 1651, 2751, 3046, 3162
AflIII	ACRYGT	3 cut(s)	223, 1311, 3291
AhlI	ACTAGT	1 cut(s)	2110
AjnI	CCWGG	6 cut(s)	1531, 1810, 1831, 2659, 3514, 3795
AleI	CACNNNNGTG	2 cut(s)	2881, 3331
Alw21I	GWGCWC	1 cut(s)	515
Aor13HI	TCCGGA	1 cut(s)	1478
AoxI	GGCC	8 cut(s)	340, 1285, 1586, 1834, 2119, 2292, 3061, 3569
ApaI	GGGCCC	1 cut(s)	1289
ApeKI	GCWGC	6 cut(s)	602, 1009, 1070, 1616, 3022, 3599
ApoI	RAATTY	5 cut(s)	1707, 1815, 1826, 2529, 3403
ArsI	GACNNNNNNTTYG	4 cut(s)	1667, 1699, 3650, 3682
AseI	ATTAAT	2 cut(s)	114, 2901
Asp700I	GAANNNNTTC	2 cut(s)	1332, 2877
AspLEI	GCGC	1 cut(s)	1631
AspS9I	GGNCC	6 cut(s)	341, 992, 1285, 1286, 2321, 3266
AsuC2I	CCSGG	3 cut(s)	381, 1270, 2394
AvaII	GGWCC	3 cut(s)	992, 2321, 3266
BaeGI	GKGCMC	1 cut(s)	1289
BalI	TGGCCA	1 cut(s)	3063
BamHI	GGATCC	1 cut(s)	964
BanI	GGYRCC	1 cut(s)	392
BanII	GRGCYC	3 cut(s)	1220, 1289, 3200
BbsI	GAAGAC	5 cut(s)	63, 1047, 1701, 2732, 3060
Bbv12I	GWGCWC	1 cut(s)	515
BbvI	GCAGC	6 cut(s)	614, 996, 1057, 1628, 3009, 3611
BceAI	ACGGC	3 cut(s)	131, 2174, 3035
BciT130I	CCWGG	6 cut(s)	1533, 1812, 1833, 2661, 3516, 3797
BciVI	GTATCC	1 cut(s)	2004
BclI	TGATCA	1 cut(s)	208
BcnI	CCSGG	3 cut(s)	381, 1270, 2394
BcuI	ACTAGT	1 cut(s)	2110
BfmI	CTRYAG	4 cut(s)	695, 1290, 2705, 3270
BfuAI	ACCTGC	4 cut(s)	654, 1090, 2756, 3070
BfuI	GTATCC	1 cut(s)	2004
BisI	GCNGC	8 cut(s)	340, 603, 1010, 1071, 1617, 2150, 3023, 3600
BlsI	GCNGC	8 cut(s)	341, 604, 1011, 1072, 1618, 2151, 3024, 3601
BmcAI	AGTACT	1 cut(s)	298
Bme1390I	CCNGG	9 cut(s)	381, 1270, 1533, 1812, 1833, 2394, 2661, 3516, 3797
Bme18I	GGWCC	3 cut(s)	992, 2321, 3266
BmgT120I	GGNCC	6 cut(s)	341, 992, 1285, 1286, 2321, 3266
BmiI	GGNNCC	6 cut(s)	343, 394, 966, 1287, 3267, 3613
BmrFI	CCNGG	9 cut(s)	381, 1270, 1533, 1812, 1833, 2394, 2661, 3516, 3797
BmrI	ACTGGG	5 cut(s)	1214, 1328, 3194, 3308, 3661
BmsI	GCATC	7 cut(s)	424, 538, 2437, 2624, 3195, 3357, 3788
BmuI	ACTGGG	5 cut(s)	1214, 1328, 3194, 3308, 3661
BoxI	GACNNNNGTC	2 cut(s)	1047, 2352
BpiI	GAAGAC	5 cut(s)	63, 1047, 1701, 2732, 3060
Bpu10I	CCTNAGC	2 cut(s)	270, 1431
BpuEI	CTTGAG	2 cut(s)	3686, 3804
BpuMI	CCSGG	3 cut(s)	381, 1270, 2394
Bsa29I	ATCGAT	1 cut(s)	3840
BsaBI	GATNNNNATC	1 cut(s)	207
BsaHI	GRCGYC	2 cut(s)	68, 176
BsaJI	CCNNGG	3 cut(s)	855, 2295, 2481
BsaWI	WCCGGW	3 cut(s)	395, 1478, 2228
BsaXI	ACNNNNNCTCC	2 cut(s)	2571, 2601
Bse118I	RCCGGY	1 cut(s)	1836
Bse1I	ACTGG	9 cut(s)	757, 1220, 1323, 2336, 2967, 3168, 3200, 3303, 3656
Bse3DI	GCAATG	3 cut(s)	765, 968, 2981
Bse8I	GATNNNNATC	1 cut(s)	207
BseAI	TCCGGA	1 cut(s)	1478
BseBI	CCWGG	6 cut(s)	1533, 1812, 1833, 2661, 3516, 3797
BseCI	ATCGAT	1 cut(s)	3840
BseDI	CCNNGG	3 cut(s)	855, 2295, 2481
BseJI	GATNNNNATC	1 cut(s)	207
BseMI	GCAATG	3 cut(s)	765, 968, 2981
BseMII	CTCAG	9 cut(s)	754, 1367, 1422, 1767, 2709, 2744, 2999, 3474, 3734
BseNI	ACTGG	9 cut(s)	757, 1220, 1323, 2336, 2967, 3168, 3200, 3303, 3656
BseSI	GKGCMC	1 cut(s)	1289
BseXI	GCAGC	6 cut(s)	614, 996, 1057, 1628, 3009, 3611
BseYI	CCCAGC	1 cut(s)	3248
BsgI	GTGCAG	1 cut(s)	288
Bsh1236I	CGCG	2 cut(s)	1631, 2812
BshFI	GGCC	8 cut(s)	342, 1287, 1588, 1836, 2121, 2294, 3063, 3571
BshNI	GGYRCC	1 cut(s)	392
BshVI	ATCGAT	1 cut(s)	3840
BsiHKAI	GWGCWC	1 cut(s)	515
BsiSI	CCGG	9 cut(s)	381, 396, 800, 1269, 1479, 1589, 1837, 2229, 2393
BsmBI	CGTCTC	4 cut(s)	154, 183, 2197, 2311
BsnI	GGCC	8 cut(s)	342, 1287, 1588, 1836, 2121, 2294, 3063, 3571
Bsp120I	GGGCCC	1 cut(s)	1285
Bsp1286I	GDGCHC	4 cut(s)	515, 1220, 1289, 3200
Bsp13I	TCCGGA	1 cut(s)	1478
Bsp19I	CCATGG	1 cut(s)	855
BspANI	GGCC	8 cut(s)	342, 1287, 1588, 1836, 2121, 2294, 3063, 3571
BspCNI	CTCAG	9 cut(s)	753, 1368, 1423, 1768, 2710, 2745, 3000, 3473, 3735
BspDI	ATCGAT	1 cut(s)	3840
BspEI	TCCGGA	1 cut(s)	1478
BspFNI	CGCG	2 cut(s)	1631, 2812
BspHI	TCATGA	5 cut(s)	205, 982, 1075, 1212, 1912
BspLI	GGNNCC	6 cut(s)	343, 394, 966, 1287, 3267, 3613
BspMAI	CTGCAG	2 cut(s)	699, 2709
BspMI	ACCTGC	4 cut(s)	654, 1090, 2756, 3070
BspQI	GCTCTTC	2 cut(s)	2253, 3540
BspT107I	GGYRCC	1 cut(s)	392
BsrBI	CCGCTC	1 cut(s)	238
BsrDI	GCAATG	3 cut(s)	765, 968, 2981
BsrFI	RCCGGY	1 cut(s)	1836
BsrI	ACTGG	9 cut(s)	757, 1220, 1323, 2336, 2967, 3168, 3200, 3303, 3656
BssAI	RCCGGY	1 cut(s)	1836
BssECI	CCNNGG	3 cut(s)	855, 2295, 2481
BssNI	GRCGYC	2 cut(s)	68, 176
BssT1I	CCWWGG	3 cut(s)	855, 2295, 2481
Bst2UI	CCWGG	6 cut(s)	1533, 1812, 1833, 2661, 3516, 3797
Bst4CI	ACNGT	6 cut(s)	309, 1090, 1210, 1654, 3330, 3579
Bst6I	CTCTTC	7 cut(s)	1064, 2145, 2253, 2968, 3077, 3540, 3797
BstACI	GRCGYC	2 cut(s)	68, 176
BstAPI	GCANNNNNTGC	3 cut(s)	587, 703, 2600
BstC8I	GCNNGC	9 cut(s)	267, 455, 583, 1014, 2246, 2292, 2468, 2596, 3027
BstDSI	CCRYGG	1 cut(s)	855
BstEII	GGTNACC	1 cut(s)	2354
BstENI	CCTNNNNNAGG	3 cut(s)	661, 1377, 3459
BstFNI	CGCG	2 cut(s)	1631, 2812
BstHHI	GCGC	1 cut(s)	1631
BstNI	CCWGG	6 cut(s)	1533, 1812, 1833, 2661, 3516, 3797
BstNSI	RCATGY	6 cut(s)	227, 585, 1315, 1460, 2598, 3295
BstPAI	GACNNNNGTC	2 cut(s)	1047, 2352
BstPI	GGTNACC	1 cut(s)	2354
BstSCI	CCNGG	9 cut(s)	379, 1268, 1531, 1810, 1831, 2392, 2659, 3514, 3795
BstSFI	CTRYAG	4 cut(s)	695, 1290, 2705, 3270
BstSLI	GKGCMC	1 cut(s)	1289
BstUI	CGCG	2 cut(s)	1631, 2812
BstV1I	GCAGC	6 cut(s)	614, 996, 1057, 1628, 3009, 3611
BstV2I	GAAGAC	5 cut(s)	63, 1047, 1701, 2732, 3060
BstX2I	RGATCY	8 cut(s)	274, 802, 964, 1322, 2299, 2815, 2977, 3039
BstXI	CCANNNNNNTGG	1 cut(s)	2331
BstYI	RGATCY	8 cut(s)	274, 802, 964, 1322, 2299, 2815, 2977, 3039
Bsu15I	ATCGAT	1 cut(s)	3840
BsuI	GTATCC	1 cut(s)	2004
BsuRI	GGCC	8 cut(s)	342, 1287, 1588, 1836, 2121, 2294, 3063, 3571
BsuTUI	ATCGAT	1 cut(s)	3840
BtgI	CCRYGG	1 cut(s)	855
BtgZI	GCGATG	2 cut(s)	518, 2786
BtsIMutI	CAGTG	3 cut(s)	2329, 2974, 3207
BveI	ACCTGC	4 cut(s)	654, 1090, 2756, 3070
Cac8I	GCNNGC	9 cut(s)	267, 455, 583, 1014, 2246, 2292, 2468, 2596, 3027
CciI	TCATGA	5 cut(s)	205, 982, 1075, 1212, 1912
CfoI	GCGC	1 cut(s)	1631
Cfr10I	RCCGGY	1 cut(s)	1836
Cfr13I	GGNCC	6 cut(s)	341, 992, 1285, 1286, 2321, 3266
ClaI	ATCGAT	1 cut(s)	3840
Csp6I	GTAC	7 cut(s)	297, 1032, 1640, 1650, 2750, 3045, 3161
CviQI	GTAC	7 cut(s)	297, 1032, 1640, 1650, 2750, 3045, 3161
DraI	TTTAAA	1 cut(s)	3102
EaeI	YGGCCR	3 cut(s)	1586, 1834, 3061
Eam1104I	CTCTTC	7 cut(s)	1064, 2145, 2253, 2968, 3077, 3540, 3797
EarI	CTCTTC	7 cut(s)	1064, 2145, 2253, 2968, 3077, 3540, 3797
Eco130I	CCWWGG	3 cut(s)	855, 2295, 2481
Eco24I	GRGCYC	3 cut(s)	1220, 1289, 3200
Eco32I	GATATC	4 cut(s)	847, 2632, 2860, 3310
Eco47I	GGWCC	3 cut(s)	992, 2321, 3266
Eco57I	CTGAAG	1 cut(s)	643
Eco91I	GGTNACC	1 cut(s)	2354
EcoNI	CCTNNNNNAGG	3 cut(s)	661, 1377, 3459
EcoO109I	RGGNCCY	3 cut(s)	1285, 1286, 3266
EcoO65I	GGTNACC	1 cut(s)	2354
EcoRI	GAATTC	1 cut(s)	1815
EcoRII	CCWGG	6 cut(s)	1531, 1810, 1831, 2659, 3514, 3795
EcoRV	GATATC	4 cut(s)	847, 2632, 2860, 3310
EcoT14I	CCWWGG	3 cut(s)	855, 2295, 2481
EcoT22I	ATGCAT	2 cut(s)	417, 2430
EcoT38I	GRGCYC	3 cut(s)	1220, 1289, 3200
ErhI	CCWWGG	3 cut(s)	855, 2295, 2481
Esp3I	CGTCTC	4 cut(s)	154, 183, 2197, 2311
FauI	CCCGC	4 cut(s)	231, 1453, 1536, 2805
FauNDI	CATATG	2 cut(s)	904, 1437
FbaI	TGATCA	1 cut(s)	208
Fnu4HI	GCNGC	8 cut(s)	340, 603, 1010, 1071, 1617, 2150, 3023, 3600
FriOI	GRGCYC	3 cut(s)	1220, 1289, 3200
Fsp4HI	GCNGC	8 cut(s)	340, 603, 1010, 1071, 1617, 2150, 3023, 3600
GlaI	GCGC	1 cut(s)	1630
GluI	GCNGC	8 cut(s)	340, 603, 1010, 1071, 1617, 2150, 3023, 3600
GsaI	CCCAGC	1 cut(s)	3252
HaeIII	GGCC	8 cut(s)	342, 1287, 1588, 1836, 2121, 2294, 3063, 3571
HapII	CCGG	9 cut(s)	381, 396, 800, 1269, 1479, 1589, 1837, 2229, 2393
HhaI	GCGC	1 cut(s)	1631
Hin1I	GRCGYC	2 cut(s)	68, 176
Hin6I	GCGC	1 cut(s)	1629
HinP1I	GCGC	1 cut(s)	1629
HincII	GTYRAC	1 cut(s)	96
HindII	GTYRAC	1 cut(s)	96
HindIII	AAGCTT	2 cut(s)	1496, 1797
HinfI	GANTC	8 cut(s)	569, 710, 1273, 1414, 2369, 2582, 3012, 3253
HpaI	GTTAAC	1 cut(s)	96
HpaII	CCGG	9 cut(s)	381, 396, 800, 1269, 1479, 1589, 1837, 2229, 2393
Hpy166II	GTNNAC	1 cut(s)	96
Hpy8I	GTNNAC	1 cut(s)	96
Hpy99I	CGWCG	4 cut(s)	1119, 1448, 2173, 3099
HpyCH4III	ACNGT	6 cut(s)	309, 1090, 1210, 1654, 3330, 3579
HpyCH4IV	ACGT	3 cut(s)	68, 176, 3660
HpySE526I	ACGT	3 cut(s)	68, 176, 3660
Hsp92I	GRCGYC	2 cut(s)	68, 176
HspAI	GCGC	1 cut(s)	1629
Kpn2I	TCCGGA	1 cut(s)	1478
Ksp22I	TGATCA	1 cut(s)	208
KspAI	GTTAAC	1 cut(s)	96
LguI	GCTCTTC	2 cut(s)	2253, 3540
LmnI	GCTCC	5 cut(s)	191, 578, 2591, 3611, 3697
Lsp1109I	GCAGC	6 cut(s)	614, 996, 1057, 1628, 3009, 3611
LweI	GCATC	7 cut(s)	424, 538, 2437, 2624, 3195, 3357, 3788
MaeII	ACGT	3 cut(s)	68, 176, 3660
MaeIII	GTNAC	9 cut(s)	309, 666, 1084, 1314, 2354, 2663, 3294, 3414, 3656
MbiI	CCGCTC	1 cut(s)	238
MfeI	CAATTG	1 cut(s)	1863
MflI	RGATCY	8 cut(s)	274, 802, 964, 1322, 2299, 2815, 2977, 3039
MhlI	GDGCHC	4 cut(s)	515, 1220, 1289, 3200
MlsI	TGGCCA	1 cut(s)	3063
MluNI	TGGCCA	1 cut(s)	3063
MlyI	GAGTC	2 cut(s)	704, 3006
Mox20I	TGGCCA	1 cut(s)	3063
Mph1103I	ATGCAT	2 cut(s)	417, 2430
MroI	TCCGGA	1 cut(s)	1478
MroXI	GAANNNNTTC	2 cut(s)	1332, 2877
MscI	TGGCCA	1 cut(s)	3063
MslI	CAYNNNNRTG	7 cut(s)	222, 868, 921, 1211, 2881, 3191, 3331
Msp20I	TGGCCA	1 cut(s)	3063
MspA1I	CMGCKG	1 cut(s)	957
MspI	CCGG	9 cut(s)	381, 396, 800, 1269, 1479, 1589, 1837, 2229, 2393
MspR9I	CCNGG	9 cut(s)	381, 1270, 1533, 1812, 1833, 2394, 2661, 3516, 3797
MunI	CAATTG	1 cut(s)	1863
MvaI	CCWGG	6 cut(s)	1533, 1812, 1833, 2661, 3516, 3797
MvnI	CGCG	2 cut(s)	1631, 2812
NciI	CCSGG	3 cut(s)	381, 1270, 2394
NcoI	CCATGG	1 cut(s)	855
NdeI	CATATG	2 cut(s)	904, 1437
NlaIV	GGNNCC	6 cut(s)	343, 394, 966, 1287, 3267, 3613
NmeAIII	GCCGAG	1 cut(s)	3763
NmuCI	GTSAC	4 cut(s)	309, 666, 2354, 3656
NsiI	ATGCAT	2 cut(s)	417, 2430
NspI	RCATGY	6 cut(s)	227, 585, 1315, 1460, 2598, 3295
OliI	CACNNNNGTG	2 cut(s)	2881, 3331
PaeI	GCATGC	2 cut(s)	585, 2598
PagI	TCATGA	5 cut(s)	205, 982, 1075, 1212, 1912
PaqCI	CACCTGC	1 cut(s)	2756
PciI	ACATGT	3 cut(s)	223, 1311, 3291
PciSI	GCTCTTC	2 cut(s)	2253, 3540
PcsI	WCGNNNNNNNCGW	1 cut(s)	3666
PdmI	GAANNNNTTC	2 cut(s)	1332, 2877
PfeI	GAWTC	6 cut(s)	569, 1273, 1414, 2369, 2582, 3253
PkrI	GCNGC	8 cut(s)	341, 604, 1011, 1072, 1618, 2151, 3024, 3601
PleI	GAGTC	2 cut(s)	704, 3006
PpsI	GAGTC	2 cut(s)	704, 3006
PpuMI	RGGWCCY	1 cut(s)	3266
PscI	ACATGT	3 cut(s)	223, 1311, 3291
PshAI	GACNNNNGTC	2 cut(s)	1047, 2352
PshBI	ATTAAT	2 cut(s)	114, 2901
PsiI	TTATAA	2 cut(s)	410, 2423
Psp5II	RGGWCCY	1 cut(s)	3266
Psp6I	CCWGG	6 cut(s)	1531, 1810, 1831, 2659, 3514, 3795
PspEI	GGTNACC	1 cut(s)	2354
PspFI	CCCAGC	1 cut(s)	3248
PspGI	CCWGG	6 cut(s)	1531, 1810, 1831, 2659, 3514, 3795
PspN4I	GGNNCC	6 cut(s)	343, 394, 966, 1287, 3267, 3613
PspOMI	GGGCCC	1 cut(s)	1285
PspPI	GGNCC	6 cut(s)	341, 992, 1285, 1286, 2321, 3266
PspPPI	RGGWCCY	1 cut(s)	3266
PsrI	GAACNNNNNNTAC	2 cut(s)	469, 501
PstI	CTGCAG	2 cut(s)	699, 2709
PsuI	RGATCY	8 cut(s)	274, 802, 964, 1322, 2299, 2815, 2977, 3039
RsaI	GTAC	7 cut(s)	298, 1033, 1641, 1651, 2751, 3046, 3162
RsaNI	GTAC	7 cut(s)	297, 1032, 1640, 1650, 2750, 3045, 3161
RseI	CAYNNNNRTG	7 cut(s)	222, 868, 921, 1211, 2881, 3191, 3331
SapI	GCTCTTC	2 cut(s)	2253, 3540
SatI	GCNGC	8 cut(s)	340, 603, 1010, 1071, 1617, 2150, 3023, 3600
Sau96I	GGNCC	6 cut(s)	341, 992, 1285, 1286, 2321, 3266
ScaI	AGTACT	1 cut(s)	298
SchI	GAGTC	2 cut(s)	704, 3006
ScrFI	CCNGG	9 cut(s)	381, 1270, 1533, 1812, 1833, 2394, 2661, 3516, 3797
SduI	GDGCHC	4 cut(s)	515, 1220, 1289, 3200
SfaNI	GCATC	7 cut(s)	424, 538, 2437, 2624, 3195, 3357, 3788
SfcI	CTRYAG	4 cut(s)	695, 1290, 2705, 3270
SinI	GGWCC	3 cut(s)	992, 2321, 3266
SmiMI	CAYNNNNRTG	7 cut(s)	222, 868, 921, 1211, 2881, 3191, 3331
SmlI	CTYRAG	2 cut(s)	3701, 3819
SmoI	CTYRAG	2 cut(s)	3701, 3819
SpeI	ACTAGT	1 cut(s)	2110
SphI	GCATGC	2 cut(s)	585, 2598
StyD4I	CCNGG	9 cut(s)	379, 1268, 1531, 1810, 1831, 2392, 2659, 3514, 3795
StyI	CCWWGG	3 cut(s)	855, 2295, 2481
TaaI	ACNGT	6 cut(s)	309, 1090, 1210, 1654, 3330, 3579
TaiI	ACGT	3 cut(s)	71, 179, 3663
TaqI	TCGA	9 cut(s)	220, 441, 475, 621, 1443, 1686, 2634, 3669, 3840
TaqII	GACCGA	1 cut(s)	2523
TatI	WGTACW	4 cut(s)	296, 1031, 2749, 3160
TauI	GCSGC	2 cut(s)	342, 2152
TfiI	GAWTC	6 cut(s)	569, 1273, 1414, 2369, 2582, 3253
TscAI	CASTG	3 cut(s)	2336, 2974, 3207
TseFI	GTSAC	4 cut(s)	309, 666, 2354, 3656
TseI	GCWGC	6 cut(s)	602, 1009, 1070, 1616, 3022, 3599
Tsp45I	GTSAC	4 cut(s)	309, 666, 2354, 3656
TspGWI	ACGGA	2 cut(s)	1886, 3032
TspRI	CASTG	3 cut(s)	2336, 2974, 3207
VpaK11BI	GGWCC	3 cut(s)	992, 2321, 3266
VspI	ATTAAT	2 cut(s)	114, 2901
XagI	CCTNNNNNAGG	3 cut(s)	661, 1377, 3459
XapI	RAATTY	5 cut(s)	1707, 1815, 1826, 2529, 3403
XbaI	TCTAGA	1 cut(s)	3121
XceI	RCATGY	6 cut(s)	227, 585, 1315, 1460, 2598, 3295
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2027, 3803
XmnI	GAANNNNTTC	2 cut(s)	1332, 2877
ZraI	GACGTC	2 cut(s)	69, 177
ZrmI	AGTACT	1 cut(s)	298
Zsp2I	ATGCAT	2 cut(s)	417, 2430

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.