AT4G00525

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001886
Physical Location & Seq
Forward (+)
231780 .. 232939
1160 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00525.1

Sequence Viewer

Length: 423 bp
ATGGAATCAATGGCTTCAGATCCCGAACTTGACATGGATGAAGGAGATCTGGAGAAATTGGAGTCAGATGTGAAGCAGATGGCCAAGAAGATCTCCGAGTACAGGCAAACCCTACCGGATCATCTCAGGAACACTCTTGATTCTGCGCTATCTTCCCACATACCCGTTTTCCCCAACATCGATTCCGGGTCGGATCCTCTTCCGTCGTCATGCCTCACTATCGCAGAAGCACAGGTACCTGGTGTGTTGGAAGAGCAAGACTCTGAAGAGAAATGGATTCAGCTCAAGGAAAGAATGTCGAGGAACGCTGCGAATATACCTAAAGTTGTAAAGAGAATGCGAGAATGTATAGAGAGTATCGACAAACTCGATTCTTTGGAAGTTACCATTCACCCGGCATTCAAAAGGCAAAGAATTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

140

Amino Acids

15.88

Weight (kDa)

4.83

Isoelectric Point (pI)

72.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 235
AccB1I GGYRCC 1 cut(s) 235
AclWI GGATC 4 cut(s) 14, 126, 188, 201
AcoI YGGCCR 1 cut(s) 81
AcuI CTGAAG 1 cut(s) 285
AfaI GTAC 2 cut(s) 101, 237
AfiI CCNNNNNNNGG 1 cut(s) 102
AgsI TTSAA 1 cut(s) 403
AjnI CCWGG 1 cut(s) 238
AluBI AGCT 1 cut(s) 283
AluI AGCT 1 cut(s) 283
AlwI GGATC 4 cut(s) 14, 126, 188, 201
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 1 cut(s) 308
Asp718I GGTACC 1 cut(s) 235
AspLEI GCGC 1 cut(s) 148
AsuC2I CCSGG 2 cut(s) 187, 395
AsuHPI GGTGA 1 cut(s) 383
BalI TGGCCA 1 cut(s) 83
BamHI GGATCC 1 cut(s) 193
BanI GGYRCC 1 cut(s) 235
BarI GAAGNNNNNNTAC 2 cut(s) 219, 251
BbvI GCAGC 1 cut(s) 295
BccI CCATC 1 cut(s) 73
BciT130I CCWGG 1 cut(s) 240
BcnI CCSGG 2 cut(s) 187, 395
BglII AGATCT 2 cut(s) 46, 90
BisI GCNGC 1 cut(s) 309
BlsI GCNGC 1 cut(s) 310
Bme1390I CCNGG 3 cut(s) 187, 240, 395
BmiI GGNNCC 2 cut(s) 195, 237
BmrFI CCNGG 3 cut(s) 187, 240, 395
BplI GAGNNNNNCTC 2 cut(s) 245, 277
BpmI CTGGAG 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 2 cut(s) 187, 395
Bsa29I ATCGAT 1 cut(s) 180
BsaWI WCCGGW 1 cut(s) 115
Bsc4I CCNNNNNNNGG 1 cut(s) 102
BseBI CCWGG 1 cut(s) 240
BseCI ATCGAT 1 cut(s) 180
BseGI GGATG 1 cut(s) 43
BseLI CCNNNNNNNGG 1 cut(s) 102
BseMII CTCAG 1 cut(s) 139
BseXI GCAGC 1 cut(s) 295
BshFI GGCC 1 cut(s) 83
BshNI GGYRCC 1 cut(s) 235
BshVI ATCGAT 1 cut(s) 180
BsiSI CCGG 3 cut(s) 116, 186, 395
BslI CCNNNNNNNGG 1 cut(s) 102
BsmI GAATGC 2 cut(s) 342, 398
BsnI GGCC 1 cut(s) 83
Bsp143I GATC 5 cut(s) 19, 46, 90, 118, 193
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 1 cut(s) 138
BspDI ATCGAT 1 cut(s) 180
BspLI GGNNCC 2 cut(s) 195, 237
BspPI GGATC 4 cut(s) 14, 126, 188, 201
BspQI GCTCTTC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 235
BssMI GATC 5 cut(s) 19, 46, 90, 118, 193
Bst2UI CCWGG 1 cut(s) 240
Bst6I CTCTTC 3 cut(s) 204, 246, 261
BstDEI CTNAG 1 cut(s) 125
BstF5I GGATG 1 cut(s) 43
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 5 cut(s) 22, 49, 93, 121, 196
BstMBI GATC 5 cut(s) 19, 46, 90, 118, 193
BstNI CCWGG 1 cut(s) 240
BstSCI CCNGG 3 cut(s) 185, 238, 393
BstV1I GCAGC 1 cut(s) 295
BstX2I RGATCY 4 cut(s) 19, 46, 90, 193
BstYI RGATCY 4 cut(s) 19, 46, 90, 193
Bsu15I ATCGAT 1 cut(s) 180
BsuRI GGCC 1 cut(s) 83
BsuTUI ATCGAT 1 cut(s) 180
BtsCI GGATG 1 cut(s) 43
CfoI GCGC 1 cut(s) 148
ClaI ATCGAT 1 cut(s) 180
CsiI ACCWGGT 1 cut(s) 238
Csp6I GTAC 2 cut(s) 100, 236
CviAII CATG 2 cut(s) 34, 210
CviJI RGCY 3 cut(s) 14, 83, 283
CviKI_1 RGCY 3 cut(s) 14, 83, 283
CviQI GTAC 2 cut(s) 100, 236
DdeI CTNAG 1 cut(s) 125
DpnI GATC 5 cut(s) 21, 48, 92, 120, 195
DpnII GATC 5 cut(s) 19, 46, 90, 118, 193
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 3 cut(s) 204, 246, 261
EarI CTCTTC 3 cut(s) 204, 246, 261
Eco57I CTGAAG 1 cut(s) 285
EcoRII CCWGG 1 cut(s) 238
FaeI CATG 2 cut(s) 37, 213
FaiI YATR 5 cut(s) 35, 161, 211, 317, 350
FatI CATG 2 cut(s) 33, 209
Fnu4HI GCNGC 1 cut(s) 309
FokI GGATG 1 cut(s) 50
Fsp4HI GCNGC 1 cut(s) 309
GlaI GCGC 1 cut(s) 147
GluI GCNGC 1 cut(s) 309
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 1 cut(s) 83
HapII CCGG 3 cut(s) 116, 186, 395
HhaI GCGC 1 cut(s) 148
Hin1II CATG 2 cut(s) 37, 213
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HinfI GANTC 7 cut(s) 5, 62, 140, 182, 260, 277, 371
HpaII CCGG 3 cut(s) 116, 186, 395
HphI GGTGA 1 cut(s) 383
Hpy188I TCNGA 5 cut(s) 19, 67, 97, 193, 265
Hpy188III TCNNGA 4 cut(s) 23, 50, 127, 137
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 1 cut(s) 35
HpyF3I CTNAG 1 cut(s) 125
Hsp92II CATG 2 cut(s) 37, 213
HspAI GCGC 1 cut(s) 146
KpnI GGTACC 1 cut(s) 239
Kzo9I GATC 5 cut(s) 19, 46, 90, 118, 193
LguI GCTCTTC 1 cut(s) 246
LpnPI CCDG 9 cut(s) 35, 88, 112, 129, 199, 218, 225, 252, 408
Lsp1109I GCAGC 1 cut(s) 295
MabI ACCWGGT 1 cut(s) 238
MaeIII GTNAC 1 cut(s) 382
MalI GATC 5 cut(s) 21, 48, 92, 120, 195
MboI GATC 5 cut(s) 19, 46, 90, 118, 193
MboII GAAGA 5 cut(s) 100, 144, 191, 263, 278
MflI RGATCY 4 cut(s) 19, 46, 90, 193
MlsI TGGCCA 1 cut(s) 83
MluCI AATT 2 cut(s) 56, 414
MluNI TGGCCA 1 cut(s) 83
MlyI GAGTC 2 cut(s) 71, 254
MmeI TCCRAC 2 cut(s) 171, 228
MnlI CCTC 3 cut(s) 207, 224, 294
Mox20I TGGCCA 1 cut(s) 83
MscI TGGCCA 1 cut(s) 83
MseI TTAA 1 cut(s) 417
Msp20I TGGCCA 1 cut(s) 83
MspI CCGG 3 cut(s) 116, 186, 395
MspR9I CCNGG 3 cut(s) 187, 240, 395
Mva1269I GAATGC 2 cut(s) 342, 398
MvaI CCWGG 1 cut(s) 240
NciI CCSGG 2 cut(s) 187, 395
NdeII GATC 5 cut(s) 19, 46, 90, 118, 193
NlaIII CATG 2 cut(s) 37, 213
NlaIV GGNNCC 2 cut(s) 195, 237
PciSI GCTCTTC 1 cut(s) 246
PcsI WCGNNNNNNNCGW 1 cut(s) 366
PctI GAATGC 2 cut(s) 342, 398
PfeI GAWTC 5 cut(s) 5, 140, 182, 277, 371
PkrI GCNGC 1 cut(s) 310
PleI GAGTC 2 cut(s) 70, 254
PpsI GAGTC 2 cut(s) 70, 254
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 2 cut(s) 195, 237
PsuI RGATCY 4 cut(s) 19, 46, 90, 193
RsaI GTAC 2 cut(s) 101, 237
RsaNI GTAC 2 cut(s) 100, 236
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 1 cut(s) 309
Sau3AI GATC 5 cut(s) 19, 46, 90, 118, 193
SchI GAGTC 2 cut(s) 71, 254
ScrFI CCNGG 3 cut(s) 187, 240, 395
SetI ASST 4 cut(s) 237, 241, 285, 322
SexAI ACCWGGT 1 cut(s) 238
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 2 cut(s) 56, 414
StyD4I CCNGG 3 cut(s) 185, 238, 393
TaqI TCGA 4 cut(s) 180, 299, 360, 369
TasI AATT 2 cut(s) 56, 414
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 5 cut(s) 5, 140, 182, 277, 371
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TseI GCWGC 1 cut(s) 308
TspDTI ATGAA 1 cut(s) 54
TspGWI ACGGA 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.