Rh1CG215400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0001886
Physical Location & Seq
Forward (+)
46381688 .. 46389775
8088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG215400.1

Sequence Viewer

Length: 255 bp
ATGGCCTCTAGCCCCAACGTCGATGAGCTGGAAGAGTTGGAGTGCCAAGTCACCGAAATGGCGGTGAAGATTCAAGAGTACCGAGCCACTATACTGGAGCAGCTCAAGAACACACTGCCTTCCATTATCGAAGCTCAGAGACCCGTTTCTGCTGATGGGTCGAAGCCTGGGACGTCCGGGGATCCGAATTCGGCGCGCATAAGATGGAAAGGATTTGTGCCGGATATCAATACATTTTGGGAGTTTCTGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

84

Amino Acids

9.46

Weight (kDa)

4.55

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 176
AccII CGCG 1 cut(s) 196
AciI CCGC 1 cut(s) 62
AclWI GGATC 2 cut(s) 176, 189
AcsI RAATTY 1 cut(s) 187
AcyI GRCGYC 1 cut(s) 173
AfaI GTAC 2 cut(s) 80, 251
AgsI TTSAA 1 cut(s) 74
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 3 cut(s) 28, 103, 134
AluI AGCT 3 cut(s) 28, 103, 134
Alw26I GTCTC 1 cut(s) 133
AlwI GGATC 2 cut(s) 176, 189
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 100
ApoI RAATTY 1 cut(s) 187
AspLEI GCGC 2 cut(s) 196, 198
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 2 cut(s) 43, 76
BamHI GGATCC 1 cut(s) 181
BbvI GCAGC 1 cut(s) 112
BccI CCATC 2 cut(s) 149, 198
BciT130I CCWGG 1 cut(s) 168
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 133
BfaI CTAG 1 cut(s) 9
BisI GCNGC 1 cut(s) 101
BlsI GCNGC 1 cut(s) 102
Bme1390I CCNGG 2 cut(s) 168, 178
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 2 cut(s) 168, 178
BpmI CTGGAG 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 178
BsaHI GRCGYC 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 133
BsaJI CCNNGG 2 cut(s) 167, 177
BsaXI ACNNNNNCTCC 1 cut(s) 233
Bse1I ACTGG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 167, 177
BseMII CTCAG 1 cut(s) 149
BseNI ACTGG 1 cut(s) 99
BsePI GCGCGC 1 cut(s) 194
BseXI GCAGC 1 cut(s) 112
Bsh1236I CGCG 1 cut(s) 196
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 2 cut(s) 177, 221
BslFI GGGAC 1 cut(s) 184
BsmAI GTCTC 1 cut(s) 133
BsmFI GGGAC 1 cut(s) 184
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 133
Bsp143I GATC 1 cut(s) 181
BspACI CCGC 1 cut(s) 62
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 148
BspFNI CGCG 1 cut(s) 196
BspLI GGNNCC 1 cut(s) 183
BspPI GGATC 2 cut(s) 176, 189
BspTNI GGTCTC 1 cut(s) 133
BsrI ACTGG 1 cut(s) 99
BssECI CCNNGG 2 cut(s) 167, 177
BssHII GCGCGC 1 cut(s) 194
BssMI GATC 1 cut(s) 181
BssNI GRCGYC 1 cut(s) 173
Bst2UI CCWGG 1 cut(s) 168
Bst6I CTCTTC 1 cut(s) 27
BstACI GRCGYC 1 cut(s) 173
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 1 cut(s) 135
BstFNI CGCG 1 cut(s) 196
BstHHI GCGC 2 cut(s) 196, 198
BstKTI GATC 1 cut(s) 184
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 1 cut(s) 181
BstNI CCWGG 1 cut(s) 168
BstSCI CCNGG 2 cut(s) 166, 176
BstUI CGCG 1 cut(s) 196
BstV1I GCAGC 1 cut(s) 112
BstX2I RGATCY 1 cut(s) 181
BstXI CCANNNNNNTGG 1 cut(s) 94
BstYI RGATCY 1 cut(s) 181
BsuRI GGCC 1 cut(s) 5
BtsI GCAGTG 1 cut(s) 113
BtsIMutI CAGTG 1 cut(s) 113
Cac8I GCNNGC 1 cut(s) 196
CfoI GCGC 2 cut(s) 196, 198
Csp6I GTAC 2 cut(s) 79, 250
CviJI RGCY 7 cut(s) 5, 12, 28, 86, 103, 134, 166
CviKI_1 RGCY 7 cut(s) 5, 12, 28, 86, 103, 134, 166
CviQI GTAC 2 cut(s) 79, 250
DdeI CTNAG 1 cut(s) 135
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Eco31I GGTCTC 1 cut(s) 133
Eco32I GATATC 1 cut(s) 226
EcoRI GAATTC 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 166
EcoRV GATATC 1 cut(s) 226
FaiI YATR 2 cut(s) 92, 200
FaqI GGGAC 1 cut(s) 184
Fnu4HI GCNGC 1 cut(s) 101
Fsp4HI GCNGC 1 cut(s) 101
FspBI CTAG 1 cut(s) 9
GlaI GCGC 2 cut(s) 195, 197
GluI GCNGC 1 cut(s) 101
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 177, 221
HhaI GCGC 2 cut(s) 196, 198
Hin1I GRCGYC 1 cut(s) 173
Hin6I GCGC 2 cut(s) 194, 196
HinP1I GCGC 2 cut(s) 194, 196
HinfI GANTC 1 cut(s) 70
HpaII CCGG 2 cut(s) 177, 221
HphI GGTGA 2 cut(s) 43, 76
Hpy188I TCNGA 2 cut(s) 138, 186
Hpy188III TCNNGA 2 cut(s) 74, 106
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 1 cut(s) 129
HpyCH4IV ACGT 2 cut(s) 18, 173
HpyF3I CTNAG 1 cut(s) 135
HpySE526I ACGT 2 cut(s) 18, 173
Hsp92I GRCGYC 1 cut(s) 173
HspAI GCGC 2 cut(s) 194, 196
Kzo9I GATC 1 cut(s) 181
LmnI GCTCC 1 cut(s) 97
LpnPI CCDG 6 cut(s) 14, 80, 153, 180, 190, 234
Lsp1109I GCAGC 1 cut(s) 112
MaeI CTAG 1 cut(s) 9
MaeII ACGT 2 cut(s) 18, 173
MaeIII GTNAC 1 cut(s) 49
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 2 cut(s) 44, 79
MflI RGATCY 1 cut(s) 181
MluCI AATT 1 cut(s) 187
MmeI TCCRAC 1 cut(s) 18
MnlI CCTC 1 cut(s) 16
MslI CAYNNNNRTG 1 cut(s) 56
MspI CCGG 2 cut(s) 177, 221
MspR9I CCNGG 2 cut(s) 168, 178
MvaI CCWGG 1 cut(s) 168
MvnI CGCG 1 cut(s) 196
NciI CCSGG 1 cut(s) 178
NdeII GATC 1 cut(s) 181
NlaIV GGNNCC 1 cut(s) 183
NmuCI GTSAC 1 cut(s) 49
PauI GCGCGC 1 cut(s) 194
PfeI GAWTC 1 cut(s) 70
PkrI GCNGC 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 1 cut(s) 183
PsuI RGATCY 1 cut(s) 181
PteI GCGCGC 1 cut(s) 194
RsaI GTAC 2 cut(s) 80, 251
RsaNI GTAC 2 cut(s) 79, 250
RseI CAYNNNNRTG 1 cut(s) 56
SatI GCNGC 1 cut(s) 101
Sau3AI GATC 1 cut(s) 181
ScrFI CCNGG 2 cut(s) 168, 178
SetI ASST 5 cut(s) 21, 30, 105, 136, 176
SmiMI CAYNNNNRTG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 1 cut(s) 187
SsiI CCGC 1 cut(s) 62
SspMI CTAG 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 166, 176
TaiI ACGT 2 cut(s) 21, 176
TaqI TCGA 3 cut(s) 21, 129, 161
TasI AATT 1 cut(s) 187
TatI WGTACW 1 cut(s) 249
TfiI GAWTC 1 cut(s) 70
TscAI CASTG 1 cut(s) 120
TseFI GTSAC 1 cut(s) 49
TseI GCWGC 1 cut(s) 100
Tsp45I GTSAC 1 cut(s) 49
TspRI CASTG 1 cut(s) 120
XapI RAATTY 1 cut(s) 187
XspI CTAG 1 cut(s) 9
ZraI GACGTC 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.