Rroxscaffold_1G00059090

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001886
Physical Location & Seq
Forward (+)
81099295 .. 81099552
258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059090.1

Sequence Viewer

Length: 258 bp
ATGGGATCCCGATCCTTTCATGAGAAGAGGTACGGAGTTCGGGTCCTCTCGATGGTGGCGACTCGGCGTCTTAGTACCGATCTGTTGCTTTGGCGGTGGATGGTGACCTCGTGTGCGCATCCTCTCCGGCGGCGGTTACGGATGCATTTGGTTTTGCCGCCGGGCTCTTTGGAATCGAGGGTTGGAAGATTTGCGTTTCGGCCTCTAGGTGGCTATGGGATTCTCGATCCGGTGTACGCCCGGCTTTATCGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

85

Amino Acids

10.0

Weight (kDa)

11.79

Isoelectric Point (pI)

84.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 117
AciI CCGC 4 cut(s) 94, 130, 133, 158
AclWI GGATC 3 cut(s) 6, 13, 221
AcyI GRCGYC 1 cut(s) 67
AfaI GTAC 3 cut(s) 32, 76, 236
AfiI CCNNNNNNNGG 2 cut(s) 52, 209
AhdI GACNNNNNGTC 1 cut(s) 66
AjuI GAANNNNNNNTTGG 2 cut(s) 165, 197
AlwI GGATC 3 cut(s) 6, 13, 221
AoxI GGCC 1 cut(s) 200
AspLEI GCGC 1 cut(s) 118
AspS9I GGNCC 1 cut(s) 43
AsuC2I CCSGG 2 cut(s) 162, 241
AsuHPI GGTGA 1 cut(s) 115
AvaII GGWCC 1 cut(s) 43
BamHI GGATCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 167
BauI CACGAG 1 cut(s) 109
BccI CCATC 2 cut(s) 46, 94
BcnI CCSGG 2 cut(s) 162, 241
BfaI CTAG 1 cut(s) 206
BisI GCNGC 2 cut(s) 131, 158
BlsI GCNGC 2 cut(s) 132, 159
Bme1390I CCNGG 2 cut(s) 162, 241
Bme18I GGWCC 1 cut(s) 43
BmeRI GACNNNNNGTC 1 cut(s) 66
BmgT120I GGNCC 1 cut(s) 43
BmiI GGNNCC 2 cut(s) 7, 44
BmrFI CCNGG 2 cut(s) 162, 241
BmsI GCATC 2 cut(s) 127, 132
BpuMI CCSGG 2 cut(s) 162, 241
BsaBI GATNNNNATC 1 cut(s) 10
BsaHI GRCGYC 1 cut(s) 67
BsaWI WCCGGW 1 cut(s) 229
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 209
Bse8I GATNNNNATC 1 cut(s) 10
BseGI GGATG 3 cut(s) 105, 118, 147
BseJI GATNNNNATC 1 cut(s) 10
BseLI CCNNNNNNNGG 2 cut(s) 52, 209
BshFI GGCC 1 cut(s) 202
BsiSI CCGG 4 cut(s) 127, 161, 230, 241
BslI CCNNNNNNNGG 2 cut(s) 52, 209
BsnI GGCC 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 167
Bsp143I GATC 4 cut(s) 5, 11, 79, 226
BspACI CCGC 4 cut(s) 94, 130, 133, 158
BspANI GGCC 1 cut(s) 202
BspHI TCATGA 1 cut(s) 19
BspLI GGNNCC 2 cut(s) 7, 44
BspPI GGATC 3 cut(s) 6, 13, 221
BssMI GATC 4 cut(s) 5, 11, 79, 226
BssNI GRCGYC 1 cut(s) 67
BssSI CACGAG 1 cut(s) 109
Bst2BI CACGAG 1 cut(s) 109
Bst6I CTCTTC 1 cut(s) 20
BstACI GRCGYC 1 cut(s) 67
BstDEI CTNAG 1 cut(s) 71
BstEII GGTNACC 1 cut(s) 103
BstF5I GGATG 3 cut(s) 105, 118, 147
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 4 cut(s) 8, 14, 82, 229
BstMBI GATC 4 cut(s) 5, 11, 79, 226
BstPI GGTNACC 1 cut(s) 103
BstSCI CCNGG 2 cut(s) 160, 239
BstX2I RGATCY 1 cut(s) 5
BstYI RGATCY 1 cut(s) 5
BsuRI GGCC 1 cut(s) 202
BtsCI GGATG 3 cut(s) 105, 118, 147
CciI TCATGA 1 cut(s) 19
CfoI GCGC 1 cut(s) 118
Cfr13I GGNCC 1 cut(s) 43
CseI GACGC 1 cut(s) 56
Csp6I GTAC 3 cut(s) 31, 75, 235
CviAII CATG 1 cut(s) 20
CviJI RGCY 4 cut(s) 165, 202, 213, 244
CviKI_1 RGCY 4 cut(s) 165, 202, 213, 244
CviQI GTAC 3 cut(s) 31, 75, 235
DdeI CTNAG 1 cut(s) 71
DpnI GATC 4 cut(s) 7, 13, 81, 228
DpnII GATC 4 cut(s) 5, 11, 79, 226
DriI GACNNNNNGTC 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 20
Eam1105I GACNNNNNGTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 167
Eco47I GGWCC 1 cut(s) 43
Eco91I GGTNACC 1 cut(s) 103
EcoO109I RGGNCCY 1 cut(s) 43
EcoO65I GGTNACC 1 cut(s) 103
EcoT22I ATGCAT 1 cut(s) 147
EcoT38I GRGCYC 1 cut(s) 167
FaeI CATG 1 cut(s) 23
FaiI YATR 2 cut(s) 21, 216
FatI CATG 1 cut(s) 19
Fnu4HI GCNGC 2 cut(s) 131, 158
FokI GGATG 3 cut(s) 105, 112, 154
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 2 cut(s) 131, 158
FspAI RTGCGCAY 1 cut(s) 117
FspBI CTAG 1 cut(s) 206
FspI TGCGCA 1 cut(s) 117
GlaI GCGC 1 cut(s) 117
GluI GCNGC 2 cut(s) 131, 158
HaeIII GGCC 1 cut(s) 202
HapII CCGG 4 cut(s) 127, 161, 230, 241
HgaI GACGC 1 cut(s) 56
HhaI GCGC 1 cut(s) 118
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 1 cut(s) 23
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HinfI GANTC 3 cut(s) 61, 173, 220
HpaII CCGG 4 cut(s) 127, 161, 230, 241
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 1 cut(s) 235
Hpy188I TCNGA 1 cut(s) 252
Hpy188III TCNNGA 4 cut(s) 9, 20, 49, 224
Hpy8I GTNNAC 1 cut(s) 235
HpyCH4V TGCA 1 cut(s) 145
HpyF3I CTNAG 1 cut(s) 71
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 1 cut(s) 23
HspAI GCGC 1 cut(s) 116
Kzo9I GATC 4 cut(s) 5, 11, 79, 226
LpnPI CCDG 4 cut(s) 140, 174, 243, 254
LweI GCATC 2 cut(s) 127, 132
MaeI CTAG 1 cut(s) 206
MaeIII GTNAC 2 cut(s) 103, 135
MalI GATC 4 cut(s) 7, 13, 81, 228
MboI GATC 4 cut(s) 5, 11, 79, 226
MboII GAAGA 2 cut(s) 37, 198
MflI RGATCY 1 cut(s) 5
MhlI GDGCHC 1 cut(s) 167
MlyI GAGTC 1 cut(s) 55
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 7 cut(s) 21, 56, 118, 132, 171, 213, 246
Mph1103I ATGCAT 1 cut(s) 147
MspI CCGG 4 cut(s) 127, 161, 230, 241
MspR9I CCNGG 2 cut(s) 162, 241
NciI CCSGG 2 cut(s) 162, 241
NdeII GATC 4 cut(s) 5, 11, 79, 226
NlaIII CATG 1 cut(s) 23
NlaIV GGNNCC 2 cut(s) 7, 44
NmeAIII GCCGAG 1 cut(s) 43
NmuCI GTSAC 1 cut(s) 103
NsbI TGCGCA 1 cut(s) 117
NsiI ATGCAT 1 cut(s) 147
PagI TCATGA 1 cut(s) 19
PcsI WCGNNNNNNNCGW 1 cut(s) 56
PfeI GAWTC 2 cut(s) 173, 220
PkrI GCNGC 2 cut(s) 132, 159
PleI GAGTC 1 cut(s) 55
PpsI GAGTC 1 cut(s) 55
PpuMI RGGWCCY 1 cut(s) 43
Psp5II RGGWCCY 1 cut(s) 43
PspEI GGTNACC 1 cut(s) 103
PspN4I GGNNCC 2 cut(s) 7, 44
PspPI GGNCC 1 cut(s) 43
PspPPI RGGWCCY 1 cut(s) 43
PsuI RGATCY 1 cut(s) 5
RsaI GTAC 3 cut(s) 32, 76, 236
RsaNI GTAC 3 cut(s) 31, 75, 235
SatI GCNGC 2 cut(s) 131, 158
Sau3AI GATC 4 cut(s) 5, 11, 79, 226
Sau96I GGNCC 1 cut(s) 43
SchI GAGTC 1 cut(s) 55
ScrFI CCNGG 2 cut(s) 162, 241
SduI GDGCHC 1 cut(s) 167
SetI ASST 4 cut(s) 32, 110, 211, 257
SfaNI GCATC 2 cut(s) 127, 132
SinI GGWCC 1 cut(s) 43
SsiI CCGC 4 cut(s) 94, 130, 133, 158
SspMI CTAG 1 cut(s) 206
StyD4I CCNGG 2 cut(s) 160, 239
TaqI TCGA 3 cut(s) 50, 176, 225
TauI GCSGC 2 cut(s) 133, 160
TfiI GAWTC 2 cut(s) 173, 220
TseFI GTSAC 1 cut(s) 103
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 1 cut(s) 8
TspGWI ACGGA 2 cut(s) 48, 154
VpaK11BI GGWCC 1 cut(s) 43
XspI CTAG 1 cut(s) 206
Zsp2I ATGCAT 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.