RLG00000028177

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001886
Physical Location & Seq
Reverse (-)
19762632 .. 19764143
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028177

Sequence Viewer

Length: 408 bp
ATGGCGGCCGCGGGATCCGAACCCGGATCCGACGCCGACGAGCTGGACGAGCTGGAGTGCCAAGTTACCGAAATGGCGGTGAAGATTCAAGAGTACCGAGCCACTATACCGGAGCAGCTCAAGAACACCTTGCCTTCCATTATCGAAGCTCAGAGGCCCGTTTTGGATGATGGGTCGGAGCCTGGGACGTCCGGGGATCCGAATTCGGGACAGATTGCTTCTGATAAGGATGCATTAGGGGATCAGACGTTTCAGAAGATAAATTTGCTTAGAGACAAAATCTCTCGTAATGTTGCTGCATTGCCGATTGTCATGAAGAGGTTGAAGGAGTGTATGTCTAATATTGACAAACTGGATTCTCAAAACACATTCATACATCCTGCATTTAAACGGAAAAGGACTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

14.81

Weight (kDa)

4.91

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 191
AccII CGCG 1 cut(s) 11
AciI CCGC 4 cut(s) 5, 9, 11, 77
AclWI GGATC 7 cut(s) 9, 21, 22, 34, 191, 204, 249
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 2 cut(s) 202, 262
AcyI GRCGYC 2 cut(s) 33, 188
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 1 cut(s) 206
AgsI TTSAA 2 cut(s) 89, 325
AjnI CCWGG 1 cut(s) 181
AluBI AGCT 5 cut(s) 43, 52, 118, 149, 405
AluI AGCT 5 cut(s) 43, 52, 118, 149, 405
Alw26I GTCTC 1 cut(s) 267
AlwI GGATC 7 cut(s) 9, 21, 22, 34, 191, 204, 249
AoxI GGCC 2 cut(s) 6, 155
ApeKI GCWGC 2 cut(s) 115, 296
ApoI RAATTY 2 cut(s) 202, 262
AspS9I GGNCC 1 cut(s) 156
AsuC2I CCSGG 2 cut(s) 24, 193
AsuHPI GGTGA 1 cut(s) 91
BamHI GGATCC 3 cut(s) 14, 26, 196
BbvI GCAGC 2 cut(s) 127, 283
BccI CCATC 1 cut(s) 164
BciT130I CCWGG 1 cut(s) 183
BcnI CCSGG 2 cut(s) 24, 193
BcoDI GTCTC 1 cut(s) 267
BfaI CTAG 2 cut(s) 402, 406
BisI GCNGC 4 cut(s) 6, 9, 116, 297
BlsI GCNGC 4 cut(s) 7, 10, 117, 298
Bme1390I CCNGG 3 cut(s) 24, 183, 193
BmgT120I GGNCC 1 cut(s) 156
BmiI GGNNCC 4 cut(s) 16, 28, 180, 198
BmrFI CCNGG 3 cut(s) 24, 183, 193
BmsI GCATC 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 104
BpuMI CCSGG 2 cut(s) 24, 193
BsaHI GRCGYC 2 cut(s) 33, 188
BsaJI CCNNGG 3 cut(s) 9, 182, 192
BsaWI WCCGGW 1 cut(s) 109
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 357
Bse3DI GCAATG 1 cut(s) 299
BseBI CCWGG 1 cut(s) 183
BseDI CCNNGG 3 cut(s) 9, 182, 192
BseGI GGATG 3 cut(s) 172, 235, 376
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMI GCAATG 1 cut(s) 299
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 1 cut(s) 357
BseX3I CGGCCG 1 cut(s) 6
BseXI GCAGC 2 cut(s) 127, 283
Bsh1236I CGCG 1 cut(s) 11
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 2 cut(s) 8, 157
BsiEI CGRYCG 1 cut(s) 9
BsiSI CCGG 3 cut(s) 24, 110, 192
BslFI GGGAC 2 cut(s) 199, 222
BslI CCNNNNNNNGG 1 cut(s) 206
BsmAI GTCTC 1 cut(s) 267
BsmFI GGGAC 2 cut(s) 199, 222
BsnI GGCC 2 cut(s) 8, 157
Bsp143I GATC 4 cut(s) 14, 26, 196, 241
BspACI CCGC 4 cut(s) 5, 9, 11, 77
BspANI GGCC 2 cut(s) 8, 157
BspCNI CTCAG 1 cut(s) 163
BspFNI CGCG 1 cut(s) 11
BspHI TCATGA 1 cut(s) 312
BspLI GGNNCC 4 cut(s) 16, 28, 180, 198
BspPI GGATC 7 cut(s) 9, 21, 22, 34, 191, 204, 249
BsrDI GCAATG 1 cut(s) 299
BsrI ACTGG 1 cut(s) 357
BssECI CCNNGG 3 cut(s) 9, 182, 192
BssMI GATC 4 cut(s) 14, 26, 196, 241
BssNI GRCGYC 2 cut(s) 33, 188
Bst2UI CCWGG 1 cut(s) 183
Bst6I CTCTTC 1 cut(s) 311
BstACI GRCGYC 2 cut(s) 33, 188
BstDEI CTNAG 2 cut(s) 150, 269
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 3 cut(s) 172, 235, 376
BstFNI CGCG 1 cut(s) 11
BstKTI GATC 4 cut(s) 17, 29, 199, 244
BstMAI GTCTC 1 cut(s) 267
BstMBI GATC 4 cut(s) 14, 26, 196, 241
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 1 cut(s) 49
BstNI CCWGG 1 cut(s) 183
BstSCI CCNGG 3 cut(s) 22, 181, 191
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 2 cut(s) 127, 283
BstX2I RGATCY 3 cut(s) 14, 26, 196
BstYI RGATCY 3 cut(s) 14, 26, 196
BstZI CGGCCG 1 cut(s) 6
BsuRI GGCC 2 cut(s) 8, 157
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 3 cut(s) 172, 235, 376
CciI TCATGA 1 cut(s) 312
CciNI GCGGCCGC 1 cut(s) 6
Cfr13I GGNCC 1 cut(s) 156
Cfr42I CCGCGG 1 cut(s) 12
CseI GACGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 94
CviAII CATG 1 cut(s) 313
CviJI RGCY 9 cut(s) 8, 43, 52, 101, 118, 149, 157, 181, 405
CviKI_1 RGCY 9 cut(s) 8, 43, 52, 101, 118, 149, 157, 181, 405
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 2 cut(s) 150, 269
DpnI GATC 4 cut(s) 16, 28, 198, 243
DpnII GATC 4 cut(s) 14, 26, 196, 241
DraI TTTAAA 1 cut(s) 388
EaeI YGGCCR 1 cut(s) 6
EagI CGGCCG 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 311
EarI CTCTTC 1 cut(s) 311
EclXI CGGCCG 1 cut(s) 6
Eco52I CGGCCG 1 cut(s) 6
EcoRI GAATTC 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 181
EcoT22I ATGCAT 1 cut(s) 235
FaeI CATG 1 cut(s) 316
FaiI YATR 4 cut(s) 107, 314, 335, 374
FalI AAGNNNNNCTT 2 cut(s) 113, 145
FaqI GGGAC 2 cut(s) 199, 222
FatI CATG 1 cut(s) 312
FauI CCCGC 1 cut(s) 4
Fnu4HI GCNGC 4 cut(s) 6, 9, 116, 297
FokI GGATG 3 cut(s) 179, 242, 363
Fsp4HI GCNGC 4 cut(s) 6, 9, 116, 297
FspBI CTAG 2 cut(s) 402, 406
GluI GCNGC 4 cut(s) 6, 9, 116, 297
GsuI CTGGAG 1 cut(s) 74
HaeIII GGCC 2 cut(s) 8, 157
HapII CCGG 3 cut(s) 24, 110, 192
HgaI GACGC 1 cut(s) 41
Hin1I GRCGYC 2 cut(s) 33, 188
Hin1II CATG 1 cut(s) 316
HinfI GANTC 2 cut(s) 85, 356
HpaII CCGG 3 cut(s) 24, 110, 192
HphI GGTGA 1 cut(s) 91
Hpy188I TCNGA 8 cut(s) 19, 31, 153, 178, 201, 223, 246, 255
Hpy188III TCNNGA 4 cut(s) 89, 121, 207, 313
Hpy99I CGWCG 2 cut(s) 35, 41
HpyAV CCTTC 2 cut(s) 144, 319
HpyCH4IV ACGT 2 cut(s) 188, 248
HpyCH4V TGCA 3 cut(s) 233, 299, 383
HpyF10VI GCNNNNNNNGC 1 cut(s) 49
HpyF3I CTNAG 2 cut(s) 150, 269
HpySE526I ACGT 2 cut(s) 188, 248
Hsp92I GRCGYC 2 cut(s) 33, 188
Hsp92II CATG 1 cut(s) 316
KspI CCGCGG 1 cut(s) 12
Kzo9I GATC 4 cut(s) 14, 26, 196, 241
LmnI GCTCC 2 cut(s) 112, 178
LpnPI CCDG 9 cut(s) 29, 37, 38, 123, 168, 195, 205, 338, 393
Lsp1109I GCAGC 2 cut(s) 127, 283
LweI GCATC 1 cut(s) 220
MaeI CTAG 2 cut(s) 402, 406
MaeII ACGT 2 cut(s) 188, 248
MaeIII GTNAC 1 cut(s) 64
MalI GATC 4 cut(s) 16, 28, 198, 243
MboI GATC 4 cut(s) 14, 26, 196, 241
MboII GAAGA 3 cut(s) 94, 268, 328
MflI RGATCY 3 cut(s) 14, 26, 196
MluCI AATT 2 cut(s) 202, 262
MmeI TCCRAC 2 cut(s) 54, 156
MnlI CCTC 2 cut(s) 147, 312
Mph1103I ATGCAT 1 cut(s) 235
MseI TTAA 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 3 cut(s) 24, 110, 192
MspR9I CCNGG 3 cut(s) 24, 183, 193
MvaI CCWGG 1 cut(s) 183
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 49
NciI CCSGG 2 cut(s) 24, 193
NdeII GATC 4 cut(s) 14, 26, 196, 241
NlaIII CATG 1 cut(s) 316
NlaIV GGNNCC 4 cut(s) 16, 28, 180, 198
NotI GCGGCCGC 1 cut(s) 6
NsiI ATGCAT 1 cut(s) 235
PagI TCATGA 1 cut(s) 312
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PfeI GAWTC 2 cut(s) 85, 356
PkrI GCNGC 4 cut(s) 7, 10, 117, 298
Psp6I CCWGG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 4 cut(s) 16, 28, 180, 198
PspPI GGNCC 1 cut(s) 156
PsuI RGATCY 3 cut(s) 14, 26, 196
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
SacII CCGCGG 1 cut(s) 12
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 4 cut(s) 6, 9, 116, 297
Sau3AI GATC 4 cut(s) 14, 26, 196, 241
Sau96I GGNCC 1 cut(s) 156
ScrFI CCNGG 3 cut(s) 24, 183, 193
SetI ASST 9 cut(s) 45, 54, 120, 131, 151, 191, 251, 323, 407
SfaNI GCATC 1 cut(s) 220
Sfr303I CCGCGG 1 cut(s) 12
SgrBI CCGCGG 1 cut(s) 12
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 2 cut(s) 202, 262
SsiI CCGC 4 cut(s) 5, 9, 11, 77
SspI AATATT 1 cut(s) 343
SspMI CTAG 2 cut(s) 402, 406
StyD4I CCNGG 3 cut(s) 22, 181, 191
TaiI ACGT 2 cut(s) 191, 251
TaqI TCGA 1 cut(s) 144
TasI AATT 2 cut(s) 202, 262
TauI GCSGC 2 cut(s) 8, 11
TfiI GAWTC 2 cut(s) 85, 356
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TseI GCWGC 2 cut(s) 115, 296
TspDTI ATGAA 2 cut(s) 329, 361
TspGWI ACGGA 1 cut(s) 406
XapI RAATTY 2 cut(s) 202, 262
XspI CTAG 2 cut(s) 402, 406
ZraI GACGTC 1 cut(s) 189
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.