MD07G1138600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001886
Physical Location & Seq
Reverse (-)
20147512 .. 20150406
2895 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1138600.v1.1.491

Sequence Viewer

Length: 384 bp
ATGGCGCTGAACTTAAGCGGATCCGATCTGGAAGATGAGGAGCTGGAGCGCCAAGTGCAGGAAATGGCGAAGAAGATTCTCGAGTACAGAGCCACTTTACCAGACCAGCTCAAGAATACCTTCGCCTCCATTCTCGCTGCTCACCGGCCCGCTTTTTCCGATAGGTCGGGTCCTGGGACGTCCGGAGCTTCGAGTTTAGATGCAGGGCAAGTTGCTTCTAATAAGGCAAGATTACTGGCTGATGGAGATCAGACTTTCGAGAAGATGCGACTGCACAAAGATAAAGTTCCCAGCAATGTTGCTGCCTTGCATATTGTTTTGAAGAGGGCGGAAGAATGTTTCTTTAAGATTGACGAATTAGATCCTCCAAAACCGAACGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

128

Amino Acids

13.96

Weight (kDa)

5.61

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 182
AccIII TCCGGA 1 cut(s) 182
AciI CCGC 3 cut(s) 18, 150, 329
AclWI GGATC 3 cut(s) 15, 28, 356
AcyI GRCGYC 1 cut(s) 179
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 58
AflII CTTAAG 1 cut(s) 13
AgsI TTSAA 1 cut(s) 322
AjnI CCWGG 1 cut(s) 172
AluBI AGCT 3 cut(s) 43, 109, 188
AluI AGCT 3 cut(s) 43, 109, 188
AlwI GGATC 3 cut(s) 15, 28, 356
Ama87I CYCGRG 1 cut(s) 80
Aor13HI TCCGGA 1 cut(s) 182
AoxI GGCC 1 cut(s) 146
ApeKI GCWGC 2 cut(s) 137, 302
Asp700I GAANNNNTTC 1 cut(s) 119
AspLEI GCGC 2 cut(s) 7, 51
AspS9I GGNCC 2 cut(s) 147, 170
AsuHPI GGTGA 1 cut(s) 134
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 1 cut(s) 170
BamHI GGATCC 1 cut(s) 20
BbvI GCAGC 2 cut(s) 124, 289
BccI CCATC 1 cut(s) 236
BciT130I CCWGG 1 cut(s) 174
BfoI RGCGCY 2 cut(s) 8, 52
BfrI CTTAAG 1 cut(s) 13
BisI GCNGC 2 cut(s) 138, 303
BlsI GCNGC 2 cut(s) 139, 304
Bme1390I CCNGG 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 170
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 2 cut(s) 147, 170
BmiI GGNNCC 2 cut(s) 22, 171
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 2 cut(s) 190, 255
BpmI CTGGAG 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 95
BsaBI GATNNNNATC 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 179
BsaJI CCNNGG 1 cut(s) 173
BsaWI WCCGGW 1 cut(s) 182
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse118I RCCGGY 1 cut(s) 144
Bse1I ACTGG 1 cut(s) 240
Bse3DI GCAATG 1 cut(s) 301
Bse8I GATNNNNATC 1 cut(s) 246
BseAI TCCGGA 1 cut(s) 182
BseBI CCWGG 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 173
BseJI GATNNNNATC 1 cut(s) 246
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMI GCAATG 1 cut(s) 301
BseNI ACTGG 1 cut(s) 240
BseRI GAGGAG 1 cut(s) 53
BseXI GCAGC 2 cut(s) 124, 289
BseYI CCCAGC 1 cut(s) 290
BsgI GTGCAG 2 cut(s) 77, 257
BshFI GGCC 1 cut(s) 148
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 2 cut(s) 145, 183
BslFI GGGAC 1 cut(s) 190
BslI CCNNNNNNNGG 1 cut(s) 58
BsmFI GGGAC 1 cut(s) 190
BsnI GGCC 1 cut(s) 148
BsoBI CYCGRG 1 cut(s) 80
Bsp13I TCCGGA 1 cut(s) 182
Bsp143I GATC 4 cut(s) 20, 25, 247, 361
BspACI CCGC 3 cut(s) 18, 150, 329
BspANI GGCC 1 cut(s) 148
BspEI TCCGGA 1 cut(s) 182
BspLI GGNNCC 2 cut(s) 22, 171
BspPI GGATC 3 cut(s) 15, 28, 356
BspTI CTTAAG 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 301
BsrFI RCCGGY 1 cut(s) 144
BsrI ACTGG 1 cut(s) 240
BssAI RCCGGY 1 cut(s) 144
BssECI CCNNGG 1 cut(s) 173
BssMI GATC 4 cut(s) 20, 25, 247, 361
BssNI GRCGYC 1 cut(s) 179
Bst2UI CCWGG 1 cut(s) 174
Bst6I CTCTTC 1 cut(s) 317
BstACI GRCGYC 1 cut(s) 179
BstAFI CTTAAG 1 cut(s) 13
BstC8I GCNNGC 1 cut(s) 150
BstH2I RGCGCY 2 cut(s) 8, 52
BstHHI GCGC 2 cut(s) 7, 51
BstKTI GATC 4 cut(s) 23, 28, 250, 364
BstMBI GATC 4 cut(s) 20, 25, 247, 361
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstNI CCWGG 1 cut(s) 174
BstSCI CCNGG 1 cut(s) 172
BstV1I GCAGC 2 cut(s) 124, 289
BstX2I RGATCY 2 cut(s) 20, 361
BstYI RGATCY 2 cut(s) 20, 361
BsuRI GGCC 1 cut(s) 148
Cac8I GCNNGC 1 cut(s) 150
CfoI GCGC 2 cut(s) 7, 51
Cfr10I RCCGGY 1 cut(s) 144
Cfr13I GGNCC 2 cut(s) 147, 170
Csp6I GTAC 1 cut(s) 85
CviJI RGCY 6 cut(s) 43, 92, 109, 148, 188, 239
CviKI_1 RGCY 6 cut(s) 43, 92, 109, 148, 188, 239
CviQI GTAC 1 cut(s) 85
DpnI GATC 4 cut(s) 22, 27, 249, 363
DpnII GATC 4 cut(s) 20, 25, 247, 361
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
EciI GGCGGA 1 cut(s) 344
Eco47I GGWCC 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 80
EcoO109I RGGNCCY 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 172
FaiI YATR 1 cut(s) 312
FalI AAGNNNNNCTT 2 cut(s) 104, 136
FaqI GGGAC 1 cut(s) 190
FauI CCCGC 1 cut(s) 157
Fnu4HI GCNGC 2 cut(s) 138, 303
Fsp4HI GCNGC 2 cut(s) 138, 303
GlaI GCGC 2 cut(s) 6, 50
GluI GCNGC 2 cut(s) 138, 303
GsaI CCCAGC 1 cut(s) 294
GsuI CTGGAG 1 cut(s) 65
HaeII RGCGCY 2 cut(s) 8, 52
HaeIII GGCC 1 cut(s) 148
HapII CCGG 2 cut(s) 145, 183
HhaI GCGC 2 cut(s) 7, 51
Hin1I GRCGYC 1 cut(s) 179
Hin6I GCGC 2 cut(s) 5, 49
HinP1I GCGC 2 cut(s) 5, 49
HinfI GANTC 1 cut(s) 76
HpaII CCGG 2 cut(s) 145, 183
HphI GGTGA 1 cut(s) 134
Hpy188I TCNGA 4 cut(s) 25, 160, 252, 383
Hpy188III TCNNGA 5 cut(s) 29, 80, 112, 183, 259
HpyAV CCTTC 1 cut(s) 130
HpyCH4IV ACGT 2 cut(s) 179, 378
HpyCH4V TGCA 4 cut(s) 58, 203, 274, 310
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpySE526I ACGT 2 cut(s) 179, 378
Hsp92I GRCGYC 1 cut(s) 179
HspAI GCGC 2 cut(s) 5, 49
Kpn2I TCCGGA 1 cut(s) 182
Kzo9I GATC 4 cut(s) 20, 25, 247, 361
LmnI GCTCC 3 cut(s) 40, 46, 185
Lsp1109I GCAGC 2 cut(s) 124, 289
LweI GCATC 2 cut(s) 190, 255
MaeII ACGT 2 cut(s) 179, 378
MalI GATC 4 cut(s) 22, 27, 249, 363
MboI GATC 4 cut(s) 20, 25, 247, 361
MboII GAAGA 6 cut(s) 44, 82, 85, 274, 334, 344
MflI RGATCY 2 cut(s) 20, 361
MluCI AATT 1 cut(s) 356
MnlI CCTC 4 cut(s) 31, 136, 318, 375
MroI TCCGGA 1 cut(s) 182
MroXI GAANNNNTTC 1 cut(s) 119
MseI TTAA 2 cut(s) 14, 345
MspCI CTTAAG 1 cut(s) 13
MspI CCGG 2 cut(s) 145, 183
MspR9I CCNGG 1 cut(s) 174
MvaI CCWGG 1 cut(s) 174
MwoI GCNNNNNNNGC 1 cut(s) 55
NdeII GATC 4 cut(s) 20, 25, 247, 361
NlaIV GGNNCC 2 cut(s) 22, 171
PaeR7I CTCGAG 1 cut(s) 80
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 1 cut(s) 76
PkrI GCNGC 2 cut(s) 139, 304
PpuMI RGGWCCY 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 170
Psp6I CCWGG 1 cut(s) 172
PspFI CCCAGC 1 cut(s) 290
PspGI CCWGG 1 cut(s) 172
PspN4I GGNNCC 2 cut(s) 22, 171
PspPI GGNCC 2 cut(s) 147, 170
PspPPI RGGWCCY 1 cut(s) 170
PsuI RGATCY 2 cut(s) 20, 361
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SaqAI TTAA 2 cut(s) 14, 345
SatI GCNGC 2 cut(s) 138, 303
Sau3AI GATC 4 cut(s) 20, 25, 247, 361
Sau96I GGNCC 2 cut(s) 147, 170
ScrFI CCNGG 1 cut(s) 174
SetI ASST 7 cut(s) 45, 111, 122, 167, 182, 190, 381
SfaNI GCATC 2 cut(s) 190, 255
Sfr274I CTCGAG 1 cut(s) 80
SinI GGWCC 1 cut(s) 170
SlaI CTCGAG 1 cut(s) 80
SmlI CTYRAG 3 cut(s) 13, 80, 110
SmoI CTYRAG 3 cut(s) 13, 80, 110
Sse9I AATT 1 cut(s) 356
SsiI CCGC 3 cut(s) 18, 150, 329
StyD4I CCNGG 1 cut(s) 172
TaiI ACGT 2 cut(s) 182, 381
TaqI TCGA 3 cut(s) 81, 191, 258
TasI AATT 1 cut(s) 356
TatI WGTACW 1 cut(s) 84
TfiI GAWTC 1 cut(s) 76
Tru1I TTAA 2 cut(s) 14, 345
Tru9I TTAA 2 cut(s) 14, 345
TseI GCWGC 2 cut(s) 137, 302
Vha464I CTTAAG 1 cut(s) 13
VpaK11BI GGWCC 1 cut(s) 170
XhoI CTCGAG 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 119
ZraI GACGTC 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.