RLG00000009237

Transmembrane protein 33 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001886
Physical Location & Seq
Reverse (-)
49539739 .. 49542545
2807 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009237

Sequence Viewer

Length: 819 bp
ATGCATTGCTGTCACACAGATTCGAAATACGACCGAAATAACAAAGAAGCACAGAAATGCTACACAGTTTTTAACTACAAATTGAAAGATTTGCATCTCCAAATCTCTCTCTCTCTCTCAACACCTCCCTCATGCGCCTCGCCGCCGCCAACTCTTCCGACCCCTCCCCTTCCTTCGAGTCCACCTTCGCCTTCCTCTCCTGCTTCCCCATCTCTAAGTCCTCCTTCAACTCCCAAGTGGACTCACCGATCCCACTCACCTCAAACCCTTCCTCGACCAAATCTCCGCTTCCATCTCCGACCTCGAGAAGCTAGTCACCGAGAACTCCTACTCCCCTCGTACAAAGTCCGATCTTCGCTGAAAACGGACGACCCACAGAAGCTGAAGAAGATAGTGGTGGCGGCTTACGAGTACGAGAACGACCTGAGATGGGCTGACTATTGGGCCATCATCCTCATCCCTCCTCACATGGCCTCTAGCCCCGACGCCGACAAGCTGGACGAGTTGGAGTGCCAAGTCATCGAAATGGCGGTAAAGATTCAAGAGTACCGAGCCACTGTACTAGAGCAGCTCATGAACACGCTGCCTTCCATTATTGAAGCTCAGAGACCCGTTTCGGCTGATGGGTCGGAGCCTGGGACGTCCGAGGATCCGAATTCGGCTGTTTGGAGCTCCGATTCGAACCCGAAATCAAGGCCGAGAGAGAAGATGATCCAATCCAGGGAGAAGCTGATCCGATCTAGGGAGAAGACGACGTCGGCCGGTGGGCTTGGGGATGTTTTTCGGTGGCAGCTGAGCGAAGATGACGTTGGCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0121

Protein Analysis

273

Amino Acids

30.6

Weight (kDa)

8.21

Isoelectric Point (pI)

72.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 644, 758
AciI CCGC 5 cut(s) 143, 146, 286, 401, 530
AclWI GGATC 5 cut(s) 243, 644, 657, 706, 727
AcoI YGGCCR 2 cut(s) 759, 811
AcsI RAATTY 1 cut(s) 655
AcuI CTGAAG 1 cut(s) 404
AcyI GRCGYC 3 cut(s) 486, 641, 755
AfaI GTAC 4 cut(s) 341, 413, 548, 561
AfiI CCNNNNNNNGG 3 cut(s) 430, 721, 742
AgsI TTSAA 4 cut(s) 85, 228, 542, 599
AjnI CCWGG 2 cut(s) 634, 719
AjuI GAANNNNNNNTTGG 1 cut(s) 792
AloI GAACNNNNNNTCC 2 cut(s) 315, 347
AluBI AGCT 8 cut(s) 311, 382, 496, 571, 602, 672, 730, 793
AluI AGCT 8 cut(s) 311, 382, 496, 571, 602, 672, 730, 793
Alw21I GWGCWC 1 cut(s) 674
Alw26I GTCTC 1 cut(s) 601
AlwI GGATC 5 cut(s) 243, 644, 657, 706, 727
AlwNI CAGNNNCTG 1 cut(s) 382
Ama87I CYCGRG 1 cut(s) 303
AoxI GGCC 5 cut(s) 444, 471, 695, 759, 811
ApeKI GCWGC 3 cut(s) 568, 583, 790
ApoI RAATTY 1 cut(s) 655
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 1 cut(s) 444
AsuHPI GGTGA 3 cut(s) 236, 249, 308
AsuII TTCGAA 2 cut(s) 23, 680
AvaI CYCGRG 1 cut(s) 303
BamHI GGATCC 1 cut(s) 649
BanII GRGCYC 1 cut(s) 674
BbsI GAAGAC 1 cut(s) 755
Bbv12I GWGCWC 1 cut(s) 674
BbvI GCAGC 3 cut(s) 570, 580, 802
BccI CCATC 5 cut(s) 217, 300, 423, 455, 617
BceAI ACGGC 1 cut(s) 798
BciT130I CCWGG 2 cut(s) 636, 721
BcoDI GTCTC 1 cut(s) 601
BfaI CTAG 4 cut(s) 312, 477, 563, 741
BisI GCNGC 6 cut(s) 143, 146, 402, 569, 584, 791
BlpI GCTNAGC 1 cut(s) 794
BlsI GCNGC 6 cut(s) 144, 147, 403, 570, 585, 792
Bme1390I CCNGG 2 cut(s) 636, 721
BmeT110I CYCGRG 1 cut(s) 303
BmgT120I GGNCC 1 cut(s) 444
BmiI GGNNCC 2 cut(s) 633, 651
BmrFI CCNGG 2 cut(s) 636, 721
BmsI GCATC 1 cut(s) 103
BpiI GAAGAC 1 cut(s) 755
Bpu1102I GCTNAGC 1 cut(s) 794
Bpu14I TTCGAA 2 cut(s) 23, 680
BsaBI GATNNNNATC 1 cut(s) 93
BsaHI GRCGYC 3 cut(s) 486, 641, 755
BsaI GGTCTC 1 cut(s) 601
BsaJI CCNNGG 3 cut(s) 635, 645, 720
BsaXI ACNNNNNCTCC 4 cut(s) 267, 297, 315, 345
Bsc4I CCNNNNNNNGG 3 cut(s) 430, 721, 742
Bse118I RCCGGY 1 cut(s) 761
Bse3DI GCAATG 1 cut(s) 4
Bse8I GATNNNNATC 1 cut(s) 93
BseBI CCWGG 2 cut(s) 636, 721
BseDI CCNNGG 3 cut(s) 635, 645, 720
BseGI GGATG 3 cut(s) 450, 456, 781
BseJI GATNNNNATC 1 cut(s) 93
BseLI CCNNNNNNNGG 3 cut(s) 430, 721, 742
BseMI GCAATG 1 cut(s) 4
BseMII CTCAG 3 cut(s) 416, 617, 785
BseRI GAGGAG 1 cut(s) 453
BseX3I CGGCCG 1 cut(s) 759
BseXI GCAGC 3 cut(s) 570, 580, 802
Bsh1285I CGRYCG 2 cut(s) 34, 762
BshFI GGCC 5 cut(s) 446, 473, 697, 761, 813
BsiEI CGRYCG 2 cut(s) 34, 762
BsiHKAI GWGCWC 1 cut(s) 674
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 1 cut(s) 762
BslFI GGGAC 1 cut(s) 652
BslI CCNNNNNNNGG 3 cut(s) 430, 721, 742
BsmAI GTCTC 1 cut(s) 601
BsmFI GGGAC 1 cut(s) 652
BsnI GGCC 5 cut(s) 446, 473, 697, 761, 813
Bso31I GGTCTC 1 cut(s) 601
BsoBI CYCGRG 1 cut(s) 303
Bsp119I TTCGAA 2 cut(s) 23, 680
Bsp1286I GDGCHC 1 cut(s) 674
Bsp143I GATC 6 cut(s) 248, 350, 649, 711, 732, 737
Bsp1720I GCTNAGC 1 cut(s) 794
BspACI CCGC 5 cut(s) 143, 146, 286, 401, 530
BspANI GGCC 5 cut(s) 446, 473, 697, 761, 813
BspCNI CTCAG 3 cut(s) 417, 616, 786
BspHI TCATGA 1 cut(s) 573
BspLI GGNNCC 2 cut(s) 633, 651
BspPI GGATC 5 cut(s) 243, 644, 657, 706, 727
BspT104I TTCGAA 2 cut(s) 23, 680
BspTNI GGTCTC 1 cut(s) 601
BsrDI GCAATG 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 761
BssAI RCCGGY 1 cut(s) 761
BssECI CCNNGG 3 cut(s) 635, 645, 720
BssMI GATC 6 cut(s) 248, 350, 649, 711, 732, 737
BssNI GRCGYC 3 cut(s) 486, 641, 755
Bst2UI CCWGG 2 cut(s) 636, 721
Bst4CI ACNGT 2 cut(s) 67, 559
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 3 cut(s) 486, 641, 755
BstBI TTCGAA 2 cut(s) 23, 680
BstDEI CTNAG 4 cut(s) 215, 425, 603, 794
BstF5I GGATG 3 cut(s) 450, 456, 781
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 6 cut(s) 251, 353, 652, 714, 735, 740
BstMAI GTCTC 1 cut(s) 601
BstMBI GATC 6 cut(s) 248, 350, 649, 711, 732, 737
BstMCI CGRYCG 2 cut(s) 34, 762
BstNI CCWGG 2 cut(s) 636, 721
BstSCI CCNGG 2 cut(s) 634, 719
BstV1I GCAGC 3 cut(s) 570, 580, 802
BstV2I GAAGAC 1 cut(s) 755
BstX2I RGATCY 1 cut(s) 649
BstYI RGATCY 1 cut(s) 649
BstZI CGGCCG 1 cut(s) 759
BsuRI GGCC 5 cut(s) 446, 473, 697, 761, 813
BtsCI GGATG 3 cut(s) 450, 456, 781
BtsIMutI CAGTG 1 cut(s) 555
CaiI CAGNNNCTG 1 cut(s) 382
CciI TCATGA 1 cut(s) 573
CfoI GCGC 1 cut(s) 137
Cfr10I RCCGGY 1 cut(s) 761
Cfr13I GGNCC 1 cut(s) 444
CseI GACGC 1 cut(s) 494
Csp6I GTAC 4 cut(s) 340, 412, 547, 560
CviAII CATG 3 cut(s) 132, 469, 574
CviQI GTAC 4 cut(s) 340, 412, 547, 560
DdeI CTNAG 4 cut(s) 215, 425, 603, 794
DpnI GATC 6 cut(s) 250, 352, 651, 713, 734, 739
DpnII GATC 6 cut(s) 248, 350, 649, 711, 732, 737
EaeI YGGCCR 2 cut(s) 759, 811
EagI CGGCCG 1 cut(s) 759
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Ecl136II GAGCTC 1 cut(s) 672
EclXI CGGCCG 1 cut(s) 759
Eco24I GRGCYC 1 cut(s) 674
Eco31I GGTCTC 1 cut(s) 601
Eco52I CGGCCG 1 cut(s) 759
Eco53kI GAGCTC 1 cut(s) 672
Eco57I CTGAAG 1 cut(s) 404
Eco88I CYCGRG 1 cut(s) 303
EcoICRI GAGCTC 1 cut(s) 672
EcoRI GAATTC 1 cut(s) 655
EcoRII CCWGG 2 cut(s) 634, 719
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 674
FaeI CATG 3 cut(s) 135, 472, 577
FaiI YATR 3 cut(s) 133, 470, 575
FalI AAGNNNNNCTT 2 cut(s) 208, 240
FaqI GGGAC 1 cut(s) 652
FatI CATG 3 cut(s) 131, 468, 573
Fnu4HI GCNGC 6 cut(s) 143, 146, 402, 569, 584, 791
FokI GGATG 3 cut(s) 437, 443, 788
FriOI GRGCYC 1 cut(s) 674
Fsp4HI GCNGC 6 cut(s) 143, 146, 402, 569, 584, 791
FspBI CTAG 4 cut(s) 312, 477, 563, 741
GlaI GCGC 1 cut(s) 136
GluI GCNGC 6 cut(s) 143, 146, 402, 569, 584, 791
HaeIII GGCC 5 cut(s) 446, 473, 697, 761, 813
HapII CCGG 1 cut(s) 762
HgaI GACGC 1 cut(s) 494
HhaI GCGC 1 cut(s) 137
Hin1I GRCGYC 3 cut(s) 486, 641, 755
Hin1II CATG 3 cut(s) 135, 472, 577
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HinfI GANTC 5 cut(s) 20, 178, 241, 538, 677
HpaII CCGG 1 cut(s) 762
HphI GGTGA 3 cut(s) 236, 249, 308
Hpy166II GTNNAC 2 cut(s) 182, 240
Hpy188I TCNGA 9 cut(s) 159, 299, 350, 606, 631, 646, 654, 676, 737
Hpy188III TCNNGA 3 cut(s) 305, 542, 574
Hpy8I GTNNAC 2 cut(s) 182, 240
Hpy99I CGWCG 3 cut(s) 488, 757, 760
HpyAV CCTTC 7 cut(s) 179, 183, 195, 201, 234, 278, 597
HpyCH4III ACNGT 2 cut(s) 67, 559
HpyCH4IV ACGT 3 cut(s) 641, 755, 807
HpyCH4V TGCA 2 cut(s) 4, 94
HpyF3I CTNAG 4 cut(s) 215, 425, 603, 794
HpySE526I ACGT 3 cut(s) 641, 755, 807
Hsp92I GRCGYC 3 cut(s) 486, 641, 755
Hsp92II CATG 3 cut(s) 135, 472, 577
HspAI GCGC 1 cut(s) 135
Kzo9I GATC 6 cut(s) 248, 350, 649, 711, 732, 737
LmnI GCTCC 3 cut(s) 631, 669, 677
LpnPI CCDG 8 cut(s) 213, 437, 482, 621, 648, 706, 733, 775
Lsp1109I GCAGC 3 cut(s) 570, 580, 802
LweI GCATC 1 cut(s) 103
MaeI CTAG 4 cut(s) 312, 477, 563, 741
MaeII ACGT 3 cut(s) 641, 755, 807
MaeIII GTNAC 2 cut(s) 11, 314
MalI GATC 6 cut(s) 250, 352, 651, 713, 734, 739
MboI GATC 6 cut(s) 248, 350, 649, 711, 732, 737
MboII GAAGA 7 cut(s) 146, 345, 397, 400, 718, 760, 812
MflI RGATCY 1 cut(s) 649
MhlI GDGCHC 1 cut(s) 674
MluCI AATT 2 cut(s) 80, 655
MlyI GAGTC 2 cut(s) 187, 235
MmeI TCCRAC 4 cut(s) 182, 322, 486, 609
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 72
MslI CAYNNNNRTG 2 cut(s) 55, 524
MspA1I CMGCKG 1 cut(s) 793
MspI CCGG 1 cut(s) 762
MspR9I CCNGG 2 cut(s) 636, 721
MvaI CCWGG 2 cut(s) 636, 721
NdeII GATC 6 cut(s) 248, 350, 649, 711, 732, 737
NlaIII CATG 3 cut(s) 135, 472, 577
NlaIV GGNNCC 2 cut(s) 633, 651
NmeAIII GCCGAG 1 cut(s) 723
NmuCI GTSAC 2 cut(s) 11, 314
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 2 cut(s) 23, 680
PaeR7I CTCGAG 1 cut(s) 303
PagI TCATGA 1 cut(s) 573
PfeI GAWTC 3 cut(s) 20, 538, 677
PflFI GACNNNGTC 1 cut(s) 754
PkrI GCNGC 6 cut(s) 144, 147, 403, 570, 585, 792
PleI GAGTC 2 cut(s) 186, 235
PpsI GAGTC 2 cut(s) 186, 235
Psp124BI GAGCTC 1 cut(s) 674
Psp6I CCWGG 2 cut(s) 634, 719
PspGI CCWGG 2 cut(s) 634, 719
PspN4I GGNNCC 2 cut(s) 633, 651
PspPI GGNCC 1 cut(s) 444
PstNI CAGNNNCTG 1 cut(s) 382
PsuI RGATCY 1 cut(s) 649
PsyI GACNNNGTC 1 cut(s) 754
PvuII CAGCTG 1 cut(s) 793
RsaI GTAC 4 cut(s) 341, 413, 548, 561
RsaNI GTAC 4 cut(s) 340, 412, 547, 560
RseI CAYNNNNRTG 2 cut(s) 55, 524
SacI GAGCTC 1 cut(s) 674
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 6 cut(s) 143, 146, 402, 569, 584, 791
Sau3AI GATC 6 cut(s) 248, 350, 649, 711, 732, 737
Sau96I GGNCC 1 cut(s) 444
SchI GAGTC 2 cut(s) 187, 235
ScrFI CCNGG 2 cut(s) 636, 721
SduI GDGCHC 1 cut(s) 674
SfaNI GCATC 1 cut(s) 103
Sfr274I CTCGAG 1 cut(s) 303
SfuI TTCGAA 2 cut(s) 23, 680
SlaI CTCGAG 1 cut(s) 303
SmiMI CAYNNNNRTG 2 cut(s) 55, 524
SmlI CTYRAG 1 cut(s) 303
SmoI CTYRAG 1 cut(s) 303
Sse9I AATT 2 cut(s) 80, 655
SsiI CCGC 5 cut(s) 143, 146, 286, 401, 530
SspMI CTAG 4 cut(s) 312, 477, 563, 741
SstI GAGCTC 1 cut(s) 674
StyD4I CCNGG 2 cut(s) 634, 719
TaaI ACNGT 2 cut(s) 67, 559
TaiI ACGT 3 cut(s) 644, 758, 810
TaqI TCGA 6 cut(s) 23, 176, 274, 304, 522, 680
TaqII GACCGA 1 cut(s) 48
TasI AATT 2 cut(s) 80, 655
TatI WGTACW 1 cut(s) 559
TauI GCSGC 3 cut(s) 145, 148, 404
TfiI GAWTC 3 cut(s) 20, 538, 677
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TscAI CASTG 1 cut(s) 562
TseFI GTSAC 2 cut(s) 11, 314
TseI GCWGC 3 cut(s) 568, 583, 790
Tsp45I GTSAC 2 cut(s) 11, 314
TspDTI ATGAA 1 cut(s) 590
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 1 cut(s) 562
Tth111I GACNNNGTC 1 cut(s) 754
XapI RAATTY 1 cut(s) 655
XhoI CTCGAG 1 cut(s) 303
XspI CTAG 4 cut(s) 312, 477, 563, 741
ZraI GACGTC 2 cut(s) 642, 756
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.