RLG00000018609

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001886
Physical Location & Seq
Forward (+)
32499259 .. 32500877
1619 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018609

Sequence Viewer

Length: 444 bp
ATGGGAAGAGGTCCTGAGTTTGGGTCTGCTCCGAGGGCGGTAATTCGGTATCCTGGTGCTGATGGGTTACTTCGGCAGTGGATGGTGACCCTATGGGTGGATCCTTTCCAGCGGCAGATCTGGTTGCTGCGGTTGGGTCTAGAGTTCGAGGGTTGGAGCACTTGGGTTAATAGATTGCTATGGGATTCCCATCCCAATGTCGGCCTAGCCTTGTCAGAGATGCAGCGTCTGATCGTCGCTGAAAACGGACGACCCACAGAAGCTGAAGAAGATAGCGGCGGCGGCTTACGACTACGAGAACGACCCGAGATGGGCAGACTACTGGCAATCCTCATCCCTCCTCACATGGACTCTAGCCCCGACGCCGACGAGCTGGACGAGTTGGAGTGCCAAGTCATCGAAATGGCCGTGAAGATTCAAGAGTACCGAGCCACTGTACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

16.72

Weight (kDa)

4.78

Isoelectric Point (pI)

47.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 38, 112, 130, 276, 279, 282
AclWI GGATC 2 cut(s) 95, 108
AcoI YGGCCR 1 cut(s) 405
AcuI CTGAAG 1 cut(s) 285
AcyI GRCGYC 1 cut(s) 363
AfaI GTAC 2 cut(s) 425, 438
AfiI CCNNNNNNNGG 4 cut(s) 20, 97, 200, 311
AgsI TTSAA 1 cut(s) 419
AjnI CCWGG 1 cut(s) 52
AluBI AGCT 2 cut(s) 263, 373
AluI AGCT 2 cut(s) 263, 373
Alw21I GWGCWC 1 cut(s) 161
AlwI GGATC 2 cut(s) 95, 108
AlwNI CAGNNNCTG 2 cut(s) 229, 263
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 2 cut(s) 202, 405
ApeKI GCWGC 2 cut(s) 127, 223
AspS9I GGNCC 1 cut(s) 11
AsuHPI GGTGA 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 1 cut(s) 11
BamHI GGATCC 1 cut(s) 100
Bbv12I GWGCWC 1 cut(s) 161
BbvI GCAGC 2 cut(s) 114, 235
BccI CCATC 4 cut(s) 56, 76, 198, 304
BceAI ACGGC 1 cut(s) 392
BciT130I CCWGG 1 cut(s) 54
BciVI GTATCC 1 cut(s) 60
BfaI CTAG 3 cut(s) 140, 206, 354
BfuI GTATCC 1 cut(s) 60
BglII AGATCT 1 cut(s) 117
BisI GCNGC 6 cut(s) 113, 128, 224, 277, 280, 283
BlsI GCNGC 6 cut(s) 114, 129, 225, 278, 281, 284
Bme1390I CCNGG 1 cut(s) 54
Bme18I GGWCC 1 cut(s) 11
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 1 cut(s) 54
BmsI GCATC 1 cut(s) 210
BsaBI GATNNNNATC 1 cut(s) 189
BsaHI GRCGYC 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 32
Bsc4I CCNNNNNNNGG 4 cut(s) 20, 97, 200, 311
Bse1I ACTGG 1 cut(s) 327
Bse8I GATNNNNATC 1 cut(s) 189
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 1 cut(s) 32
BseGI GGATG 3 cut(s) 87, 190, 333
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 4 cut(s) 20, 97, 200, 311
BseMII CTCAG 1 cut(s) 6
BseNI ACTGG 1 cut(s) 327
BseRI GAGGAG 1 cut(s) 330
BseXI GCAGC 2 cut(s) 114, 235
BshFI GGCC 2 cut(s) 204, 407
BsiHKAI GWGCWC 1 cut(s) 161
BsiHKCI CYCGRG 1 cut(s) 305
BslI CCNNNNNNNGG 4 cut(s) 20, 97, 200, 311
BsnI GGCC 2 cut(s) 204, 407
BsoBI CYCGRG 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 161
Bsp143I GATC 3 cut(s) 100, 117, 231
BspACI CCGC 6 cut(s) 38, 112, 130, 276, 279, 282
BspANI GGCC 2 cut(s) 204, 407
BspCNI CTCAG 1 cut(s) 7
BspLI GGNNCC 1 cut(s) 102
BspPI GGATC 2 cut(s) 95, 108
BsrI ACTGG 1 cut(s) 327
BssECI CCNNGG 1 cut(s) 32
BssMI GATC 3 cut(s) 100, 117, 231
BssNI GRCGYC 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 1 cut(s) 436
BstACI GRCGYC 1 cut(s) 363
BstDEI CTNAG 1 cut(s) 15
BstEII GGTNACC 1 cut(s) 85
BstF5I GGATG 3 cut(s) 87, 190, 333
BstKTI GATC 3 cut(s) 103, 120, 234
BstMBI GATC 3 cut(s) 100, 117, 231
BstMWI GCNNNNNNNGC 2 cut(s) 35, 282
BstNI CCWGG 1 cut(s) 54
BstPI GGTNACC 1 cut(s) 85
BstSCI CCNGG 1 cut(s) 52
BstV1I GCAGC 2 cut(s) 114, 235
BstX2I RGATCY 2 cut(s) 100, 117
BstYI RGATCY 2 cut(s) 100, 117
BsuI GTATCC 1 cut(s) 60
BsuRI GGCC 2 cut(s) 204, 407
BtsCI GGATG 3 cut(s) 87, 190, 333
BtsI GCAGTG 1 cut(s) 83
BtsIMutI CAGTG 2 cut(s) 83, 432
CaiI CAGNNNCTG 2 cut(s) 229, 263
Cfr13I GGNCC 1 cut(s) 11
CseI GACGC 2 cut(s) 215, 371
Csp6I GTAC 2 cut(s) 424, 437
CviAII CATG 1 cut(s) 346
CviJI RGCY 8 cut(s) 204, 209, 263, 285, 357, 373, 407, 431
CviKI_1 RGCY 8 cut(s) 204, 209, 263, 285, 357, 373, 407, 431
CviQI GTAC 2 cut(s) 424, 437
DdeI CTNAG 1 cut(s) 15
DpnI GATC 3 cut(s) 102, 119, 233
DpnII GATC 3 cut(s) 100, 117, 231
EaeI YGGCCR 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 285
Eco88I CYCGRG 1 cut(s) 305
Eco91I GGTNACC 1 cut(s) 85
EcoO109I RGGNCCY 1 cut(s) 11
EcoO65I GGTNACC 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 52
FaeI CATG 1 cut(s) 349
FaiI YATR 4 cut(s) 94, 181, 347, 442
FatI CATG 1 cut(s) 345
Fnu4HI GCNGC 6 cut(s) 113, 128, 224, 277, 280, 283
FokI GGATG 3 cut(s) 94, 177, 320
Fsp4HI GCNGC 6 cut(s) 113, 128, 224, 277, 280, 283
FspBI CTAG 3 cut(s) 140, 206, 354
GluI GCNGC 6 cut(s) 113, 128, 224, 277, 280, 283
HaeIII GGCC 2 cut(s) 204, 407
HgaI GACGC 2 cut(s) 215, 371
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 1 cut(s) 349
HinfI GANTC 3 cut(s) 185, 350, 415
HphI GGTGA 1 cut(s) 97
Hpy188I TCNGA 3 cut(s) 33, 217, 231
Hpy188III TCNNGA 3 cut(s) 14, 140, 419
Hpy99I CGWCG 3 cut(s) 239, 365, 371
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4V TGCA 1 cut(s) 223
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 282
HpyF3I CTNAG 1 cut(s) 15
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 1 cut(s) 349
Kzo9I GATC 3 cut(s) 100, 117, 231
LmnI GCTCC 2 cut(s) 34, 156
LpnPI CCDG 7 cut(s) 27, 39, 66, 106, 122, 308, 359
Lsp1109I GCAGC 2 cut(s) 114, 235
LweI GCATC 1 cut(s) 210
MaeI CTAG 3 cut(s) 140, 206, 354
MaeIII GTNAC 2 cut(s) 66, 85
MalI GATC 3 cut(s) 102, 119, 233
MboI GATC 3 cut(s) 100, 117, 231
MboII GAAGA 4 cut(s) 18, 278, 281, 424
MflI RGATCY 2 cut(s) 100, 117
MhlI GDGCHC 1 cut(s) 161
MluCI AATT 1 cut(s) 42
MlyI GAGTC 1 cut(s) 344
MmeI TCCRAC 2 cut(s) 134, 363
MnlI CCTC 5 cut(s) 27, 142, 341, 348, 351
MseI TTAA 1 cut(s) 168
MslI CAYNNNNRTG 2 cut(s) 195, 401
MspA1I CMGCKG 1 cut(s) 112
MspR9I CCNGG 1 cut(s) 54
MvaI CCWGG 1 cut(s) 54
MwoI GCNNNNNNNGC 2 cut(s) 35, 282
NdeII GATC 3 cut(s) 100, 117, 231
NlaIII CATG 1 cut(s) 349
NlaIV GGNNCC 1 cut(s) 102
NmuCI GTSAC 1 cut(s) 85
PcsI WCGNNNNNNNCGW 2 cut(s) 375, 405
PfeI GAWTC 2 cut(s) 185, 415
PkrI GCNGC 6 cut(s) 114, 129, 225, 278, 281, 284
PleI GAGTC 1 cut(s) 344
PpsI GAGTC 1 cut(s) 344
PpuMI RGGWCCY 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 11
Psp6I CCWGG 1 cut(s) 52
PspEI GGTNACC 1 cut(s) 85
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 1 cut(s) 102
PspPI GGNCC 1 cut(s) 11
PspPPI RGGWCCY 1 cut(s) 11
PstNI CAGNNNCTG 2 cut(s) 229, 263
PsuI RGATCY 2 cut(s) 100, 117
RsaI GTAC 2 cut(s) 425, 438
RsaNI GTAC 2 cut(s) 424, 437
RseI CAYNNNNRTG 2 cut(s) 195, 401
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 6 cut(s) 113, 128, 224, 277, 280, 283
Sau3AI GATC 3 cut(s) 100, 117, 231
Sau96I GGNCC 1 cut(s) 11
SchI GAGTC 1 cut(s) 344
ScrFI CCNGG 1 cut(s) 54
SduI GDGCHC 1 cut(s) 161
SetI ASST 3 cut(s) 13, 265, 375
SfaNI GCATC 1 cut(s) 210
SinI GGWCC 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 195, 401
Sse9I AATT 1 cut(s) 42
SsiI CCGC 6 cut(s) 38, 112, 130, 276, 279, 282
SspMI CTAG 3 cut(s) 140, 206, 354
StyD4I CCNGG 1 cut(s) 52
TaaI ACNGT 1 cut(s) 436
TaqI TCGA 2 cut(s) 147, 399
TasI AATT 1 cut(s) 42
TauI GCSGC 4 cut(s) 115, 279, 282, 285
TfiI GAWTC 2 cut(s) 185, 415
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 2 cut(s) 83, 439
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 2 cut(s) 127, 223
Tsp45I GTSAC 1 cut(s) 85
TspGWI ACGGA 1 cut(s) 261
TspRI CASTG 2 cut(s) 83, 439
VpaK11BI GGWCC 1 cut(s) 11
XbaI TCTAGA 1 cut(s) 139
XspI CTAG 3 cut(s) 140, 206, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.