pycom07g13580

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001886
Physical Location & Seq
Reverse (-)
15458297 .. 15460883
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g13580.2

Sequence Viewer

Length: 498 bp
ATGGCAAAAAGAGTAGATAGTACAAAATTACCCTCCAAAACGAAACCTCAACCTGATTTCACGCTCTCACTCTTACCCCTCTGCATTAGTGCATTTGAAGGAATGGCGCTGAATTTAAGCGGATCCGATCTGGAAGATGAGGAGCTGGAGCGCCAAGTGCAGGAAATGGCGAAGAAGATTCTCGAGTACAGAGCCATTTTACCAGACCAGCTCAAGAATACCTTCGCCTCCATTCTCGCTGCTCACCGGCCCGCTATTTCGGATAGGTCGGGTCCTGGGACGTCCGGAGCTTCGAGTTTAGGGCAAGTTGCTTCTAAGAAGGCGGGATTACTGGCTGATGGAGATCAGACTTTCGAGAAGATGCGACTGCACAAAGATAAAGTTCCCAGCAATGTCGCTGCCTTGCATATTGTTTTGAAGAGGGCGGAAGAATGTTTCTTTAAGATTGACAAATTAGATCCTCAAAACCGAACGTCTCAGAACAAAGGGACTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

18.04

Weight (kDa)

8.87

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 284
AccIII TCCGGA 1 cut(s) 284
AciI CCGC 4 cut(s) 120, 252, 323, 425
AclWI GGATC 3 cut(s) 117, 130, 452
AcsI RAATTY 1 cut(s) 112
AcyI GRCGYC 1 cut(s) 281
AfaI GTAC 2 cut(s) 22, 188
AfiI CCNNNNNNNGG 1 cut(s) 160
AgsI TTSAA 2 cut(s) 98, 418
AhlI ACTAGT 1 cut(s) 491
AjnI CCWGG 1 cut(s) 274
AluBI AGCT 3 cut(s) 145, 211, 290
AluI AGCT 3 cut(s) 145, 211, 290
Alw26I GTCTC 1 cut(s) 480
AlwI GGATC 3 cut(s) 117, 130, 452
Ama87I CYCGRG 1 cut(s) 182
Aor13HI TCCGGA 1 cut(s) 284
AoxI GGCC 1 cut(s) 248
ApeKI GCWGC 2 cut(s) 239, 398
ApoI RAATTY 1 cut(s) 112
Asp700I GAANNNNTTC 1 cut(s) 221
AspLEI GCGC 2 cut(s) 109, 153
AspS9I GGNCC 2 cut(s) 249, 272
AsuHPI GGTGA 1 cut(s) 236
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 1 cut(s) 272
BamHI GGATCC 1 cut(s) 122
BbvI GCAGC 2 cut(s) 226, 385
BccI CCATC 1 cut(s) 332
BciT130I CCWGG 1 cut(s) 276
BcoDI GTCTC 1 cut(s) 480
BcuI ACTAGT 1 cut(s) 491
BfaI CTAG 1 cut(s) 492
BfoI RGCGCY 2 cut(s) 110, 154
BisI GCNGC 2 cut(s) 240, 399
BlsI GCNGC 2 cut(s) 241, 400
Bme1390I CCNGG 1 cut(s) 276
Bme18I GGWCC 1 cut(s) 272
BmeT110I CYCGRG 1 cut(s) 182
BmgT120I GGNCC 2 cut(s) 249, 272
BmiI GGNNCC 2 cut(s) 124, 273
BmrFI CCNGG 1 cut(s) 276
BmsI GCATC 1 cut(s) 351
BpmI CTGGAG 1 cut(s) 167
BpuEI CTTGAG 1 cut(s) 197
BsaBI GATNNNNATC 1 cut(s) 342
BsaHI GRCGYC 1 cut(s) 281
BsaJI CCNNGG 1 cut(s) 275
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse118I RCCGGY 1 cut(s) 246
Bse1I ACTGG 1 cut(s) 336
Bse3DI GCAATG 1 cut(s) 397
Bse8I GATNNNNATC 1 cut(s) 342
BseAI TCCGGA 1 cut(s) 284
BseBI CCWGG 1 cut(s) 276
BseDI CCNNGG 1 cut(s) 275
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMI GCAATG 1 cut(s) 397
BseMII CTCAG 1 cut(s) 491
BseNI ACTGG 1 cut(s) 336
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 2 cut(s) 226, 385
BseYI CCCAGC 1 cut(s) 386
BsgI GTGCAG 2 cut(s) 179, 353
BshFI GGCC 1 cut(s) 250
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 2 cut(s) 247, 285
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 1 cut(s) 160
BsmAI GTCTC 1 cut(s) 480
BsmBI CGTCTC 1 cut(s) 480
BsmFI GGGAC 1 cut(s) 292
BsnI GGCC 1 cut(s) 250
BsoBI CYCGRG 1 cut(s) 182
Bsp13I TCCGGA 1 cut(s) 284
Bsp143I GATC 4 cut(s) 122, 127, 343, 457
BspACI CCGC 4 cut(s) 120, 252, 323, 425
BspANI GGCC 1 cut(s) 250
BspCNI CTCAG 1 cut(s) 490
BspEI TCCGGA 1 cut(s) 284
BspLI GGNNCC 2 cut(s) 124, 273
BspPI GGATC 3 cut(s) 117, 130, 452
BsrDI GCAATG 1 cut(s) 397
BsrFI RCCGGY 1 cut(s) 246
BsrI ACTGG 1 cut(s) 336
BssAI RCCGGY 1 cut(s) 246
BssECI CCNNGG 1 cut(s) 275
BssMI GATC 4 cut(s) 122, 127, 343, 457
BssNI GRCGYC 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 276
Bst6I CTCTTC 1 cut(s) 413
BstACI GRCGYC 1 cut(s) 281
BstC8I GCNNGC 1 cut(s) 252
BstDEI CTNAG 2 cut(s) 315, 477
BstH2I RGCGCY 2 cut(s) 110, 154
BstHHI GCGC 2 cut(s) 109, 153
BstKTI GATC 4 cut(s) 125, 130, 346, 460
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 4 cut(s) 122, 127, 343, 457
BstMWI GCNNNNNNNGC 1 cut(s) 157
BstNI CCWGG 1 cut(s) 276
BstSCI CCNGG 1 cut(s) 274
BstV1I GCAGC 2 cut(s) 226, 385
BstX2I RGATCY 2 cut(s) 122, 457
BstYI RGATCY 2 cut(s) 122, 457
BsuRI GGCC 1 cut(s) 250
Cac8I GCNNGC 1 cut(s) 252
CfoI GCGC 2 cut(s) 109, 153
Cfr10I RCCGGY 1 cut(s) 246
Cfr13I GGNCC 2 cut(s) 249, 272
Csp6I GTAC 2 cut(s) 21, 187
CviJI RGCY 6 cut(s) 145, 194, 211, 250, 290, 335
CviKI_1 RGCY 6 cut(s) 145, 194, 211, 250, 290, 335
CviQI GTAC 2 cut(s) 21, 187
DdeI CTNAG 2 cut(s) 315, 477
DpnI GATC 4 cut(s) 124, 129, 345, 459
DpnII GATC 4 cut(s) 122, 127, 343, 457
Eam1104I CTCTTC 1 cut(s) 413
EarI CTCTTC 1 cut(s) 413
EciI GGCGGA 1 cut(s) 440
Eco47I GGWCC 1 cut(s) 272
Eco88I CYCGRG 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 274
Esp3I CGTCTC 1 cut(s) 480
FaiI YATR 1 cut(s) 408
FalI AAGNNNNNCTT 2 cut(s) 206, 238
FaqI GGGAC 1 cut(s) 292
FauI CCCGC 2 cut(s) 259, 316
Fnu4HI GCNGC 2 cut(s) 240, 399
Fsp4HI GCNGC 2 cut(s) 240, 399
FspBI CTAG 1 cut(s) 492
GlaI GCGC 2 cut(s) 108, 152
GluI GCNGC 2 cut(s) 240, 399
GsaI CCCAGC 1 cut(s) 390
GsuI CTGGAG 1 cut(s) 167
HaeII RGCGCY 2 cut(s) 110, 154
HaeIII GGCC 1 cut(s) 250
HapII CCGG 2 cut(s) 247, 285
HhaI GCGC 2 cut(s) 109, 153
Hin1I GRCGYC 1 cut(s) 281
Hin6I GCGC 2 cut(s) 107, 151
HinP1I GCGC 2 cut(s) 107, 151
HinfI GANTC 1 cut(s) 178
HpaII CCGG 2 cut(s) 247, 285
HphI GGTGA 1 cut(s) 236
Hpy188I TCNGA 4 cut(s) 127, 262, 348, 480
Hpy188III TCNNGA 5 cut(s) 131, 182, 214, 285, 355
HpyAV CCTTC 3 cut(s) 92, 232, 313
HpyCH4IV ACGT 2 cut(s) 281, 473
HpyCH4V TGCA 5 cut(s) 84, 92, 160, 370, 406
HpyF10VI GCNNNNNNNGC 1 cut(s) 157
HpyF3I CTNAG 2 cut(s) 315, 477
HpySE526I ACGT 2 cut(s) 281, 473
Hsp92I GRCGYC 1 cut(s) 281
HspAI GCGC 2 cut(s) 107, 151
Kpn2I TCCGGA 1 cut(s) 284
Kzo9I GATC 4 cut(s) 122, 127, 343, 457
LmnI GCTCC 3 cut(s) 142, 148, 287
Lsp1109I GCAGC 2 cut(s) 226, 385
LweI GCATC 1 cut(s) 351
MaeI CTAG 1 cut(s) 492
MaeII ACGT 2 cut(s) 281, 473
MalI GATC 4 cut(s) 124, 129, 345, 459
MboI GATC 4 cut(s) 122, 127, 343, 457
MboII GAAGA 6 cut(s) 146, 184, 187, 370, 430, 440
MflI RGATCY 2 cut(s) 122, 457
MluCI AATT 3 cut(s) 26, 112, 452
MnlI CCTC 7 cut(s) 43, 57, 89, 133, 238, 414, 471
MroI TCCGGA 1 cut(s) 284
MroXI GAANNNNTTC 1 cut(s) 221
MseI TTAA 2 cut(s) 116, 441
MspI CCGG 2 cut(s) 247, 285
MspR9I CCNGG 1 cut(s) 276
MvaI CCWGG 1 cut(s) 276
MwoI GCNNNNNNNGC 1 cut(s) 157
NdeII GATC 4 cut(s) 122, 127, 343, 457
NlaIV GGNNCC 2 cut(s) 124, 273
PaeR7I CTCGAG 1 cut(s) 182
PdmI GAANNNNTTC 1 cut(s) 221
PfeI GAWTC 1 cut(s) 178
PkrI GCNGC 2 cut(s) 241, 400
PpuMI RGGWCCY 1 cut(s) 272
Psp5II RGGWCCY 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 274
PspFI CCCAGC 1 cut(s) 386
PspGI CCWGG 1 cut(s) 274
PspN4I GGNNCC 2 cut(s) 124, 273
PspPI GGNCC 2 cut(s) 249, 272
PspPPI RGGWCCY 1 cut(s) 272
PsuI RGATCY 2 cut(s) 122, 457
RsaI GTAC 2 cut(s) 22, 188
RsaNI GTAC 2 cut(s) 21, 187
SaqAI TTAA 2 cut(s) 116, 441
SatI GCNGC 2 cut(s) 240, 399
Sau3AI GATC 4 cut(s) 122, 127, 343, 457
Sau96I GGNCC 2 cut(s) 249, 272
ScrFI CCNGG 1 cut(s) 276
SetI ASST 9 cut(s) 49, 55, 147, 213, 224, 269, 284, 292, 476
SfaNI GCATC 1 cut(s) 351
Sfr274I CTCGAG 1 cut(s) 182
SinI GGWCC 1 cut(s) 272
SlaI CTCGAG 1 cut(s) 182
SmlI CTYRAG 2 cut(s) 182, 212
SmoI CTYRAG 2 cut(s) 182, 212
SpeI ACTAGT 1 cut(s) 491
Sse9I AATT 3 cut(s) 26, 112, 452
SsiI CCGC 4 cut(s) 120, 252, 323, 425
SspMI CTAG 1 cut(s) 492
StyD4I CCNGG 1 cut(s) 274
TaiI ACGT 2 cut(s) 284, 476
TaqI TCGA 3 cut(s) 183, 293, 354
TasI AATT 3 cut(s) 26, 112, 452
TatI WGTACW 2 cut(s) 20, 186
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 2 cut(s) 116, 441
Tru9I TTAA 2 cut(s) 116, 441
TseI GCWGC 2 cut(s) 239, 398
VpaK11BI GGWCC 1 cut(s) 272
XapI RAATTY 1 cut(s) 112
XhoI CTCGAG 1 cut(s) 182
XmnI GAANNNNTTC 1 cut(s) 221
XspI CTAG 1 cut(s) 492
ZraI GACGTC 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.