Rroxscaffold_6G00399350

Transmembrane protein 33 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001886
Physical Location & Seq
Forward (+)
21270964 .. 21271740
777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00399350.1

Sequence Viewer

Length: 489 bp
ATGCGAGTACCGTGCACCGGGGACGGGACAAGAGCGGATGGAGTCGGGTGTGCAGACCAAGTTCGAGACTCGCGGCACCGTAACAAGCTTAAGCTTGGGGTGTTACAAAATCTTCGCCTCTATCTCCGACCTCGAGAAGCTAGTCGCCGAGAACTCCTACTCCCCTCGTACAAAGTCCGATCGTCGTTGAAAACGACGACCCACGAAGCGAAGAAGATAGCGGCGGCTGCTTACGACTACGAGAACCACCCGAGATGGGCCGACTATCGGGCCAACATCCTCATCCCTCCTCACATTTCCTTTAGCACCGACGCCGACGAGCCGGACGAGTTGGAGTGCCAAGTCACCGAAATGGGGGTGAAGATTCAAGAGTACCGAGCCACCGTACTGCAGCAGCTCAAGAACACGCCGCCTTCCATTATCAATGCTCGAGACCCGTTTCGGGCGATGGGTCGAAGCCGGGATGTCCGGGGATCCGAATTCGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0121

Protein Analysis

162

Amino Acids

18.41

Weight (kDa)

9.4

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 75
AccBSI CCGCTC 1 cut(s) 35
AccII CGCG 1 cut(s) 73
AciI CCGC 5 cut(s) 35, 73, 221, 224, 410
AclWI GGATC 2 cut(s) 468, 481
AcsI RAATTY 1 cut(s) 479
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 4 cut(s) 9, 170, 374, 387
AfiI CCNNNNNNNGG 7 cut(s) 17, 24, 256, 267, 354, 442, 483
AflII CTTAAG 1 cut(s) 89
AgsI TTSAA 2 cut(s) 190, 368
AloI GAACNNNNNNTCC 2 cut(s) 144, 176
AluBI AGCT 4 cut(s) 88, 94, 140, 397
AluI AGCT 4 cut(s) 88, 94, 140, 397
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 2 cut(s) 60, 426
Alw44I GTGCAC 1 cut(s) 13
AlwI GGATC 2 cut(s) 468, 481
Ama87I CYCGRG 3 cut(s) 132, 250, 429
AoxI GGCC 2 cut(s) 258, 270
ApaLI GTGCAC 1 cut(s) 13
ApeKI GCWGC 3 cut(s) 227, 391, 394
ApoI RAATTY 1 cut(s) 479
AspS9I GGNCC 2 cut(s) 258, 270
AsuC2I CCSGG 3 cut(s) 19, 461, 470
AsuHPI GGTGA 2 cut(s) 337, 370
AvaI CYCGRG 3 cut(s) 132, 250, 429
BaeGI GKGCMC 1 cut(s) 17
BamHI GGATCC 1 cut(s) 473
BanI GGYRCC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 17
BbvI GCAGC 3 cut(s) 214, 403, 406
BccI CCATC 3 cut(s) 32, 249, 442
BcgI CGANNNNNNTGC 1 cut(s) 28
BcnI CCSGG 3 cut(s) 19, 461, 470
BcoDI GTCTC 2 cut(s) 60, 426
BfaI CTAG 1 cut(s) 141
BfmI CTRYAG 1 cut(s) 389
BfrI CTTAAG 1 cut(s) 89
BisI GCNGC 7 cut(s) 74, 222, 225, 228, 392, 395, 410
BlsI GCNGC 7 cut(s) 75, 223, 226, 229, 393, 396, 411
Bme1390I CCNGG 3 cut(s) 19, 461, 470
BmeT110I CYCGRG 3 cut(s) 132, 250, 429
BmgT120I GGNCC 2 cut(s) 258, 270
BmiI GGNNCC 2 cut(s) 77, 475
BmrFI CCNGG 3 cut(s) 19, 461, 470
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 3 cut(s) 19, 461, 470
BsaHI GRCGYC 1 cut(s) 312
BsaI GGTCTC 1 cut(s) 426
BsaJI CCNNGG 2 cut(s) 18, 469
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bsc4I CCNNNNNNNGG 7 cut(s) 17, 24, 256, 267, 354, 442, 483
BseDI CCNNGG 2 cut(s) 18, 469
BseGI GGATG 4 cut(s) 43, 276, 282, 469
BseLI CCNNNNNNNGG 7 cut(s) 17, 24, 256, 267, 354, 442, 483
BseRI GAGGAG 1 cut(s) 279
BseSI GKGCMC 1 cut(s) 17
BseXI GCAGC 3 cut(s) 214, 403, 406
BsgI GTGCAG 1 cut(s) 72
Bsh1236I CGCG 1 cut(s) 73
Bsh1285I CGRYCG 1 cut(s) 182
BshFI GGCC 2 cut(s) 260, 272
BshNI GGYRCC 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 182
BsiHKAI GWGCWC 1 cut(s) 17
BsiHKCI CYCGRG 3 cut(s) 132, 250, 429
BsiSI CCGG 4 cut(s) 18, 323, 460, 469
BslFI GGGAC 2 cut(s) 35, 40
BslI CCNNNNNNNGG 7 cut(s) 17, 24, 256, 267, 354, 442, 483
BsmAI GTCTC 2 cut(s) 60, 426
BsmFI GGGAC 2 cut(s) 35, 40
BsnI GGCC 2 cut(s) 260, 272
Bso31I GGTCTC 1 cut(s) 426
BsoBI CYCGRG 3 cut(s) 132, 250, 429
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 2 cut(s) 179, 473
BspACI CCGC 5 cut(s) 35, 73, 221, 224, 410
BspANI GGCC 2 cut(s) 260, 272
BspFNI CGCG 1 cut(s) 73
BspLI GGNNCC 2 cut(s) 77, 475
BspMAI CTGCAG 1 cut(s) 393
BspPI GGATC 2 cut(s) 468, 481
BspT107I GGYRCC 1 cut(s) 75
BspTI CTTAAG 1 cut(s) 89
BspTNI GGTCTC 1 cut(s) 426
BsrBI CCGCTC 1 cut(s) 35
BssECI CCNNGG 2 cut(s) 18, 469
BssMI GATC 2 cut(s) 179, 473
BssNI GRCGYC 1 cut(s) 312
Bst4CI ACNGT 3 cut(s) 12, 80, 385
BstACI GRCGYC 1 cut(s) 312
BstAFI CTTAAG 1 cut(s) 89
BstF5I GGATG 4 cut(s) 43, 276, 282, 469
BstFNI CGCG 1 cut(s) 73
BstKTI GATC 2 cut(s) 182, 476
BstMAI GTCTC 2 cut(s) 60, 426
BstMBI GATC 2 cut(s) 179, 473
BstMCI CGRYCG 1 cut(s) 182
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstSCI CCNGG 3 cut(s) 17, 459, 468
BstSFI CTRYAG 1 cut(s) 389
BstSLI GKGCMC 1 cut(s) 17
BstUI CGCG 1 cut(s) 73
BstV1I GCAGC 3 cut(s) 214, 403, 406
BstX2I RGATCY 1 cut(s) 473
BstYI RGATCY 1 cut(s) 473
BsuRI GGCC 2 cut(s) 260, 272
BtgZI GCGATG 1 cut(s) 461
BtsCI GGATG 4 cut(s) 43, 276, 282, 469
Cfr13I GGNCC 2 cut(s) 258, 270
CseI GACGC 1 cut(s) 320
Csp6I GTAC 4 cut(s) 8, 169, 373, 386
CviQI GTAC 4 cut(s) 8, 169, 373, 386
DpnI GATC 2 cut(s) 181, 475
DpnII GATC 2 cut(s) 179, 473
Eco31I GGTCTC 1 cut(s) 426
Eco88I CYCGRG 3 cut(s) 132, 250, 429
EcoRI GAATTC 1 cut(s) 479
FaqI GGGAC 2 cut(s) 35, 40
Fnu4HI GCNGC 7 cut(s) 74, 222, 225, 228, 392, 395, 410
FokI GGATG 4 cut(s) 50, 263, 269, 476
Fsp4HI GCNGC 7 cut(s) 74, 222, 225, 228, 392, 395, 410
FspBI CTAG 1 cut(s) 141
GluI GCNGC 7 cut(s) 74, 222, 225, 228, 392, 395, 410
HaeIII GGCC 2 cut(s) 260, 272
HapII CCGG 4 cut(s) 18, 323, 460, 469
HgaI GACGC 1 cut(s) 320
Hin1I GRCGYC 1 cut(s) 312
HindIII AAGCTT 2 cut(s) 86, 92
HinfI GANTC 3 cut(s) 42, 68, 364
HpaII CCGG 4 cut(s) 18, 323, 460, 469
HphI GGTGA 2 cut(s) 337, 370
Hpy166II GTNNAC 1 cut(s) 15
Hpy188I TCNGA 3 cut(s) 128, 179, 478
Hpy188III TCNNGA 5 cut(s) 65, 134, 368, 400, 431
Hpy8I GTNNAC 1 cut(s) 15
Hpy99I CGWCG 4 cut(s) 187, 199, 314, 320
HpyAV CCTTC 1 cut(s) 423
HpyCH4III ACNGT 3 cut(s) 12, 80, 385
HpyCH4V TGCA 3 cut(s) 15, 53, 391
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
Hsp92I GRCGYC 1 cut(s) 312
Kzo9I GATC 2 cut(s) 179, 473
LpnPI CCDG 4 cut(s) 31, 336, 473, 482
Lsp1109I GCAGC 3 cut(s) 214, 403, 406
MaeI CTAG 1 cut(s) 141
MaeIII GTNAC 3 cut(s) 80, 102, 343
MalI GATC 2 cut(s) 181, 475
MbiI CCGCTC 1 cut(s) 35
MboI GATC 2 cut(s) 179, 473
MboII GAAGA 4 cut(s) 104, 223, 226, 373
MflI RGATCY 1 cut(s) 473
MhlI GDGCHC 1 cut(s) 17
MluCI AATT 1 cut(s) 479
MlyI GAGTC 2 cut(s) 51, 62
MmeI TCCRAC 2 cut(s) 151, 312
MnlI CCTC 6 cut(s) 128, 141, 175, 290, 297, 300
MseI TTAA 1 cut(s) 90
MslI CAYNNNNRTG 1 cut(s) 350
MspCI CTTAAG 1 cut(s) 89
MspI CCGG 4 cut(s) 18, 323, 460, 469
MspR9I CCNGG 3 cut(s) 19, 461, 470
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 1 cut(s) 227
NciI CCSGG 3 cut(s) 19, 461, 470
NdeII GATC 2 cut(s) 179, 473
NlaIV GGNNCC 2 cut(s) 77, 475
NmeAIII GCCGAG 1 cut(s) 173
NmuCI GTSAC 1 cut(s) 343
PaeR7I CTCGAG 2 cut(s) 132, 429
PcsI WCGNNNNNNNCGW 2 cut(s) 191, 324
PfeI GAWTC 1 cut(s) 364
PkrI GCNGC 7 cut(s) 75, 223, 226, 229, 393, 396, 411
Ple19I CGATCG 1 cut(s) 182
PleI GAGTC 2 cut(s) 50, 62
PpsI GAGTC 2 cut(s) 50, 62
PspN4I GGNNCC 2 cut(s) 77, 475
PspPI GGNCC 2 cut(s) 258, 270
PstI CTGCAG 1 cut(s) 393
PsuI RGATCY 1 cut(s) 473
PvuI CGATCG 1 cut(s) 182
RsaI GTAC 4 cut(s) 9, 170, 374, 387
RsaNI GTAC 4 cut(s) 8, 169, 373, 386
RseI CAYNNNNRTG 1 cut(s) 350
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 7 cut(s) 74, 222, 225, 228, 392, 395, 410
Sau3AI GATC 2 cut(s) 179, 473
Sau96I GGNCC 2 cut(s) 258, 270
SchI GAGTC 2 cut(s) 51, 62
ScrFI CCNGG 3 cut(s) 19, 461, 470
SduI GDGCHC 1 cut(s) 17
SetI ASST 5 cut(s) 90, 96, 133, 142, 399
SfcI CTRYAG 1 cut(s) 389
Sfr274I CTCGAG 2 cut(s) 132, 429
SlaI CTCGAG 2 cut(s) 132, 429
SmiMI CAYNNNNRTG 1 cut(s) 350
SmlI CTYRAG 4 cut(s) 89, 132, 398, 429
SmoI CTYRAG 4 cut(s) 89, 132, 398, 429
Sse9I AATT 1 cut(s) 479
SsiI CCGC 5 cut(s) 35, 73, 221, 224, 410
SspMI CTAG 1 cut(s) 141
StyD4I CCNGG 3 cut(s) 17, 459, 468
TaaI ACNGT 3 cut(s) 12, 80, 385
TaqI TCGA 4 cut(s) 64, 133, 430, 454
TasI AATT 1 cut(s) 479
TauI GCSGC 4 cut(s) 76, 224, 227, 412
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TseFI GTSAC 1 cut(s) 343
TseI GCWGC 3 cut(s) 227, 391, 394
Tsp45I GTSAC 1 cut(s) 343
Vha464I CTTAAG 1 cut(s) 89
VneI GTGCAC 1 cut(s) 13
XapI RAATTY 1 cut(s) 479
XhoI CTCGAG 2 cut(s) 132, 429
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.