AT4G33720

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001302
Physical Location & Seq
Forward (+)
16182743 .. 16183619
877 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33720.1

Sequence Viewer

Length: 492 bp
ATGAAAATCTTTAACTCATCTCAAAACCTATTCTTGGCCATAACCTTTTTCCTTGTTCTCATAGTTCATCTAAAAGCCCAAGACAGTCCTCAAGACTTTTTGGCAGTTCACAACCGAGCACGAGCCGAGGTTGGGGTGGGACCCTTAAGATGGGACGAGAAGGTGGCTGCTTATGCCCGTAACTATGCTAACCAGCGTAAAGGTGACTGCGCTATGAAACACTCGAGCGGGTCCTATGGAGAGAACATCGCTTGGAGCAGCGGTAGCATGACAGGCGTAGCAGCAGTTGACATGTGGGTGGACGAGCAATTTGACTACGATTATGATTCCAATACATGTGCATGGGACAAACAGTGTGGCCACTATACTCAGGTTGTGTGGAGAAACTCGGAGAGGTTGGGATGTGCAAAAGTGAGATGCAACAATGGTCAAACCTTTATCACTTGCAACTACGATCCTCCCGGTAACTGGGTTGGCGAGTGGCCTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

163

Amino Acids

18.49

Weight (kDa)

6.05

Isoelectric Point (pI)

33.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 34 - 151 4.9e-21 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 228
AciI CCGC 2 cut(s) 228, 261
AclWI GGATC 1 cut(s) 449
AcoI YGGCCR 2 cut(s) 36, 358
AfiI CCNNNNNNNGG 4 cut(s) 34, 132, 150, 468
AflII CTTAAG 1 cut(s) 145
AflIII ACRYGT 2 cut(s) 291, 335
Alw21I GWGCWC 1 cut(s) 121
AlwI GGATC 1 cut(s) 449
Ama87I CYCGRG 1 cut(s) 223
AoxI GGCC 3 cut(s) 36, 358, 482
ApeKI GCWGC 3 cut(s) 167, 258, 281
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 2 cut(s) 140, 231
AsuC2I CCSGG 1 cut(s) 462
AsuHPI GGTGA 1 cut(s) 215
AvaI CYCGRG 1 cut(s) 223
AvaII GGWCC 2 cut(s) 140, 231
BalI TGGCCA 2 cut(s) 38, 360
BauI CACGAG 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 121
BbvI GCAGC 3 cut(s) 154, 270, 293
BccI CCATC 1 cut(s) 144
BcnI CCSGG 1 cut(s) 462
BfrI CTTAAG 1 cut(s) 145
BisI GCNGC 3 cut(s) 168, 259, 282
BlsI GCNGC 3 cut(s) 169, 260, 283
Bme1390I CCNGG 1 cut(s) 462
Bme18I GGWCC 2 cut(s) 140, 231
BmeT110I CYCGRG 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 140, 231
BmiI GGNNCC 3 cut(s) 141, 142, 232
BmrFI CCNGG 1 cut(s) 462
BmrI ACTGGG 1 cut(s) 478
BmsI GCATC 1 cut(s) 407
BmuI ACTGGG 1 cut(s) 478
BpuEI CTTGAG 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 462
BsaJI CCNNGG 1 cut(s) 126
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 132, 150, 468
Bse1I ACTGG 1 cut(s) 473
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 1 cut(s) 407
BseLI CCNNNNNNNGG 4 cut(s) 34, 132, 150, 468
BseMII CTCAG 1 cut(s) 383
BseNI ACTGG 1 cut(s) 473
BseXI GCAGC 3 cut(s) 154, 270, 293
BshFI GGCC 3 cut(s) 38, 360, 484
BsiHKAI GWGCWC 1 cut(s) 121
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 1 cut(s) 462
BslFI GGGAC 3 cut(s) 153, 167, 359
BslI CCNNNNNNNGG 4 cut(s) 34, 132, 150, 468
BsmFI GGGAC 3 cut(s) 153, 167, 359
BsnI GGCC 3 cut(s) 38, 360, 484
BsoBI CYCGRG 1 cut(s) 223
Bsp1286I GDGCHC 1 cut(s) 121
Bsp143I GATC 1 cut(s) 454
BspACI CCGC 2 cut(s) 228, 261
BspANI GGCC 3 cut(s) 38, 360, 484
BspCNI CTCAG 1 cut(s) 382
BspLI GGNNCC 3 cut(s) 141, 142, 232
BspPI GGATC 1 cut(s) 449
BspTI CTTAAG 1 cut(s) 145
BsrBI CCGCTC 1 cut(s) 228
BsrI ACTGG 1 cut(s) 473
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 1 cut(s) 454
BssSI CACGAG 1 cut(s) 120
Bst2BI CACGAG 1 cut(s) 120
Bst4CI ACNGT 2 cut(s) 86, 354
BstAFI CTTAAG 1 cut(s) 145
BstDEI CTNAG 1 cut(s) 369
BstF5I GGATG 1 cut(s) 407
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 1 cut(s) 457
BstMBI GATC 1 cut(s) 454
BstMWI GCNNNNNNNGC 3 cut(s) 173, 264, 273
BstNSI RCATGY 2 cut(s) 295, 339
BstSCI CCNGG 1 cut(s) 460
BstV1I GCAGC 3 cut(s) 154, 270, 293
BsuRI GGCC 3 cut(s) 38, 360, 484
BtgZI GCGATG 1 cut(s) 232
BtsCI GGATG 1 cut(s) 407
BtsIMutI CAGTG 1 cut(s) 359
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 2 cut(s) 140, 231
CspCI CAANNNNNGTGG 2 cut(s) 337, 372
CviAII CATG 4 cut(s) 268, 292, 336, 342
CviJI RGCY 6 cut(s) 38, 77, 125, 167, 360, 484
CviKI_1 RGCY 6 cut(s) 38, 77, 125, 167, 360, 484
DdeI CTNAG 1 cut(s) 369
DpnI GATC 1 cut(s) 456
DpnII GATC 1 cut(s) 454
EaeI YGGCCR 2 cut(s) 36, 358
Eco47I GGWCC 2 cut(s) 140, 231
Eco88I CYCGRG 1 cut(s) 223
EcoO109I RGGNCCY 2 cut(s) 140, 231
FaeI CATG 4 cut(s) 271, 295, 339, 345
FaqI GGGAC 3 cut(s) 153, 167, 359
FatI CATG 4 cut(s) 267, 291, 335, 341
FauI CCCGC 1 cut(s) 221
Fnu4HI GCNGC 3 cut(s) 168, 259, 282
FokI GGATG 1 cut(s) 414
Fsp4HI GCNGC 3 cut(s) 168, 259, 282
GlaI GCGC 1 cut(s) 211
GluI GCNGC 3 cut(s) 168, 259, 282
HaeIII GGCC 3 cut(s) 38, 360, 484
HapII CCGG 1 cut(s) 462
HhaI GCGC 1 cut(s) 212
Hin1II CATG 4 cut(s) 271, 295, 339, 345
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 1 cut(s) 326
HpaII CCGG 1 cut(s) 462
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 3 cut(s) 109, 289, 301
Hpy188I TCNGA 1 cut(s) 391
Hpy188III TCNNGA 1 cut(s) 92
Hpy8I GTNNAC 3 cut(s) 109, 289, 301
HpyAV CCTTC 1 cut(s) 154
HpyCH4III ACNGT 2 cut(s) 86, 354
HpyCH4V TGCA 4 cut(s) 341, 407, 420, 447
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 264, 273
HpyF3I CTNAG 1 cut(s) 369
Hsp92II CATG 4 cut(s) 271, 295, 339, 345
HspAI GCGC 1 cut(s) 210
KflI GGGWCCC 1 cut(s) 140
Kzo9I GATC 1 cut(s) 454
LmnI GCTCC 1 cut(s) 255
LpnPI CCDG 5 cut(s) 206, 258, 356, 454, 475
Lsp1109I GCAGC 3 cut(s) 154, 270, 293
LweI GCATC 1 cut(s) 407
MaeIII GTNAC 3 cut(s) 179, 203, 464
MalI GATC 1 cut(s) 456
MbiI CCGCTC 1 cut(s) 228
MboI GATC 1 cut(s) 454
MhlI GDGCHC 1 cut(s) 121
MlsI TGGCCA 2 cut(s) 38, 360
MluCI AATT 1 cut(s) 308
MluNI TGGCCA 2 cut(s) 38, 360
MnlI CCTC 4 cut(s) 99, 121, 387, 468
Mox20I TGGCCA 2 cut(s) 38, 360
MscI TGGCCA 2 cut(s) 38, 360
MseI TTAA 2 cut(s) 12, 146
MslI CAYNNNNRTG 2 cut(s) 296, 340
Msp20I TGGCCA 2 cut(s) 38, 360
MspA1I CMGCKG 1 cut(s) 261
MspCI CTTAAG 1 cut(s) 145
MspI CCGG 1 cut(s) 462
MspR9I CCNGG 1 cut(s) 462
MwoI GCNNNNNNNGC 3 cut(s) 173, 264, 273
NciI CCSGG 1 cut(s) 462
NdeII GATC 1 cut(s) 454
NlaIII CATG 4 cut(s) 271, 295, 339, 345
NlaIV GGNNCC 3 cut(s) 141, 142, 232
NmeAIII GCCGAG 1 cut(s) 151
NmuCI GTSAC 1 cut(s) 203
NspI RCATGY 2 cut(s) 295, 339
PaeR7I CTCGAG 1 cut(s) 223
PciI ACATGT 2 cut(s) 291, 335
PfeI GAWTC 1 cut(s) 326
PkrI GCNGC 3 cut(s) 169, 260, 283
PpuMI RGGWCCY 2 cut(s) 140, 231
PscI ACATGT 2 cut(s) 291, 335
Psp5II RGGWCCY 2 cut(s) 140, 231
PspN4I GGNNCC 3 cut(s) 141, 142, 232
PspPI GGNCC 2 cut(s) 140, 231
PspPPI RGGWCCY 2 cut(s) 140, 231
PspXI VCTCGAGB 1 cut(s) 223
RseI CAYNNNNRTG 2 cut(s) 296, 340
SaqAI TTAA 2 cut(s) 12, 146
SatI GCNGC 3 cut(s) 168, 259, 282
Sau3AI GATC 1 cut(s) 454
Sau96I GGNCC 2 cut(s) 140, 231
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 1 cut(s) 121
SetI ASST 8 cut(s) 30, 47, 132, 165, 205, 375, 398, 437
SfaNI GCATC 1 cut(s) 407
Sfr274I CTCGAG 1 cut(s) 223
SinI GGWCC 2 cut(s) 140, 231
SlaI CTCGAG 1 cut(s) 223
SmiMI CAYNNNNRTG 2 cut(s) 296, 340
SmlI CTYRAG 3 cut(s) 90, 145, 223
SmoI CTYRAG 3 cut(s) 90, 145, 223
Sse9I AATT 1 cut(s) 308
SsiI CCGC 2 cut(s) 228, 261
StyD4I CCNGG 1 cut(s) 460
TaaI ACNGT 2 cut(s) 86, 354
TaqI TCGA 1 cut(s) 224
TasI AATT 1 cut(s) 308
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 2 cut(s) 12, 146
Tru9I TTAA 2 cut(s) 12, 146
TscAI CASTG 1 cut(s) 359
TseFI GTSAC 1 cut(s) 203
TseI GCWGC 3 cut(s) 167, 258, 281
Tsp45I GTSAC 1 cut(s) 203
TspDTI ATGAA 3 cut(s) 17, 56, 230
TspRI CASTG 1 cut(s) 359
Vha464I CTTAAG 1 cut(s) 145
VpaK11BI GGWCC 2 cut(s) 140, 231
XceI RCATGY 2 cut(s) 295, 339
XhoI CTCGAG 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.