Rorug05G0527900

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001302
Physical Location & Seq
Reverse (-)
71646312 .. 71649619
3308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0527900.1

Sequence Viewer

Length: 1617 bp
ATGATGCGCAGCAGCAGCTCAAGAGGTATGCTTTCTTCTCTCTCCGTCAACAATGTCATCATGGCTTTGTTTCATTCGAGACAGAGACAAACCCAAACCCATGTAGAGAGGCTTGTGAGAAAACCACCCTCAAAGCCCAAAGTTGAGGAGGATGCCTTGAAGTTGTTCCGTGAAATGCTTCACAGCCGTCCTCTGCCTTGTGTTGTTCGTTTCACTCAGTTATTGGGTCAACTTGTCAAATTGAAACATTATTCTGCCGTCATCACTTTGAATAGACAAATGCTTCTCTCTGGAATTGTTCCTGATCACTACACCCTGTCTATTATCATCAACTGCTATTGCCATTTGAATCAAATGGGGTTTGGCTTATCTGTCTTTGGAACATTCTTCAAATTGGGTCTTCAACCAAATGTTATTACCTTCAACACTCTAATCAACGGCTTTGTTCTCAAGAATCAGGTGCCTGAGGCTGCACGAGTTTTCAGCAAAATGGTGAAGACAGGTCATTGTAAGCCGGATGTGTTTACTTTCAACACACTAATAAAGGGCTTTTGCATGATAGGAAACAACAGTGCTGCTATTCGGTTACTTGGAAAGATGGAAGAAAACGGTTGTAAGCCTAACATAGTTTCCTATAACACCATCATCGACTGTCTTTGTAAGGATACACTAATTGATGAAGCATTCAACCTCTTCTCACAACTGATTAGTAGAGGTATTGCTCCAAACATTGTTACTTACAGCACTTTGATTGACGGAGTCTGCAAACTAGGCCAGTGGAAACAAGCTACAAGGCTGTTGGATGAAATGCTGAGTAAAGGTATCTTTCCGGATCTCCTCACTTTCAATGTCTTGGTTGATACATTTTGTAAGGAAGGGATGGTTGTGGAAGCCAAAAGTGTGGTTCAAATGATGATTCAAAGACATATTGAACCTAATGTCGTTACATACAACTCACTTATGGATGGTTACTGTTTGCGAGGAGAAATGGACGAGGCAAGACAAGTTTTTGATCTTATGATTAGCAGGGGCTCCATGGTTAATGTTTGGAGTTGTAGCATATTGATAAACGGATATTGCAAGCAGAAAAAGATCGATCAGGCTAACAAGATTTTCAAGGAAATGCGTCGTATGGAACTTGTTCCCAATACCGTTACTTATACCACTCTTATTGATGGTTTTTGCAAAGCAGGGAAAATTCAAGAAGCAGAAAAGTTGTTCTCTGAGATGCTAGGTTGTGGCCAGCTTCCAGATGTTCAAACTTATGCTGTTTTACTTCATGGCCTGTGTAACAACCAGCAACTTTCTACGGCAATAGAATTGCTTAGAGAAATGGAAATCAACAAAGGCCTTTGTCATCATGGCTTAATAATTGAAGCAGAAAAGCTGCTGAGAGAAATGGGAGGGGAAGGATGTTCTCCAGATGGTTGTACCTATAACACCATTATCCGAGGGTTGTTAAATAACAGTGAGACATCATGGGCTATGAAACTTATTCAAGAAATGCTTGAGAGGGATTTCTCTGCAGATGCATCAACTATGGAATTGATTATTGATTTATTATCGAAGGATATAGTAGATCCGGTTTTGTTGCCGATGTTAAAAGATCCACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

538

Amino Acids

60.6

Weight (kDa)

8.31

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 66 - 115 1.2e-06 PPR repeat family
PPR_3 PF13812 131 - 183 2.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 133 - 165 9.6e-09 PPR repeat
PPR_2 PF13041 136 - 185 7.5e-16 PPR repeat family
PPR_1 PF12854 170 - 201 5.3e-11 PPR repeat
PPR_3 PF13812 171 - 218 1.1e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 172 - 221 7.7e-17 PPR repeat family
TPR_24 PF23276 173 - 265 1.4e-06 Fungal tetratrico peptide repeats
PPR PF01535 175 - 205 9.1e-06 PPR repeat
PPR_long PF17177 192 - 320 3.9e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 203 - 234 3.7e-12 PPR repeat
PPR_2 PF13041 207 - 256 1.7e-18 PPR repeat family
PPR PF01535 210 - 240 3.4e-06 PPR repeat
PPR_3 PF13812 232 - 290 2.3e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 238 - 270 5.6e-12 PPR repeat
PPR_2 PF13041 242 - 291 1.2e-16 PPR repeat family
PPR PF01535 245 - 275 1.5e-07 PPR repeat
MRP-S27 PF10037 246 - 310 1.1e-07 Mitochondrial 28S ribosomal protein S27
PPR_1 PF12854 273 - 305 2.7e-08 PPR repeat
PPR_2 PF13041 281 - 325 3.6e-12 PPR repeat family
TPR_24 PF23276 290 - 409 2.1e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 309 - 340 1.1e-12 PPR repeat
PPR_2 PF13041 312 - 361 8.7e-16 PPR repeat family
PPR PF01535 315 - 344 2.6e-09 PPR repeat
PPR_3 PF13812 336 - 392 1.2e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 345 - 376 2.2e-06 PPR repeat
PPR_2 PF13041 348 - 396 2.4e-17 PPR repeat family
PPR PF01535 351 - 378 1.7e-06 PPR repeat
PPR_long PF17177 361 - 446 9e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 379 - 410 1.5e-15 PPR repeat
PPR_2 PF13041 382 - 431 6.7e-19 PPR repeat family
PPR PF01535 385 - 414 1.7e-10 PPR repeat
PPR_1 PF12854 414 - 446 2.2e-07 PPR repeat
PPR_2 PF13041 449 - 487 3.1e-08 PPR repeat family
PPR_2 PF13041 477 - 522 1.4e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 8
AccB1I GGYRCC 1 cut(s) 460
AccIII TCCGGA 1 cut(s) 829
AclWI GGATC 3 cut(s) 840, 1574, 1601
AcoI YGGCCR 1 cut(s) 1240
AcsI RAATTY 1 cut(s) 1197
AfaI GTAC 1 cut(s) 1432
AluBI AGCT 4 cut(s) 18, 788, 1246, 1387
AluI AGCT 4 cut(s) 18, 788, 1246, 1387
Alw26I GTCTC 3 cut(s) 73, 79, 1466
AlwI GGATC 3 cut(s) 840, 1574, 1601
Aor13HI TCCGGA 1 cut(s) 829
AoxI GGCC 4 cut(s) 772, 1240, 1282, 1348
ApeKI GCWGC 6 cut(s) 9, 12, 15, 470, 575, 1387
ApoI RAATTY 1 cut(s) 1197
Asp700I GAANNNNTTC 3 cut(s) 164, 177, 1140
AspLEI GCGC 1 cut(s) 9
AsuHPI GGTGA 1 cut(s) 505
AxyI CCTNAGG 1 cut(s) 465
BalI TGGCCA 1 cut(s) 1242
BanI GGYRCC 1 cut(s) 460
BanII GRGCYC 1 cut(s) 1034
BarI GAAGNNNNNNTAC 2 cut(s) 19, 51
BauI CACGAG 1 cut(s) 474
BbsI GAAGAC 2 cut(s) 392, 503
BbvI GCAGC 6 cut(s) 21, 24, 27, 457, 562, 1374
BccI CCATC 6 cut(s) 592, 650, 874, 959, 1169, 1418
BceAI ACGGC 4 cut(s) 171, 242, 454, 1326
BciVI GTATCC 1 cut(s) 658
BclI TGATCA 1 cut(s) 304
BcoDI GTCTC 3 cut(s) 73, 79, 1466
BfaI CTAG 2 cut(s) 770, 1232
BfmI CTRYAG 1 cut(s) 1524
BfuI GTATCC 1 cut(s) 658
BisI GCNGC 6 cut(s) 10, 13, 16, 471, 576, 1388
BlsI GCNGC 6 cut(s) 11, 14, 17, 472, 577, 1389
BmiI GGNNCC 2 cut(s) 462, 1033
BmsI GCATC 4 cut(s) 142, 1218, 1519, 1541
BpiI GAAGAC 2 cut(s) 392, 503
BpmI CTGGAG 1 cut(s) 1404
BpuEI CTTGAG 3 cut(s) 4, 434, 1529
Bsa29I ATCGAT 1 cut(s) 1095
BsaJI CCNNGG 2 cut(s) 1035, 1450
BsaWI WCCGGW 2 cut(s) 829, 1582
Bse1I ACTGG 1 cut(s) 775
Bse21I CCTNAGG 1 cut(s) 465
BseAI TCCGGA 1 cut(s) 829
BseCI ATCGAT 1 cut(s) 1095
BseDI CCNNGG 2 cut(s) 1035, 1450
BseGI GGATG 6 cut(s) 157, 523, 808, 885, 970, 1418
BseMII CTCAG 5 cut(s) 230, 456, 803, 1215, 1382
BseNI ACTGG 1 cut(s) 775
BseRI GAGGAG 3 cut(s) 161, 827, 996
BseXI GCAGC 6 cut(s) 21, 24, 27, 457, 562, 1374
BsgI GTGCAG 1 cut(s) 456
BshFI GGCC 4 cut(s) 774, 1242, 1284, 1350
BshNI GGYRCC 1 cut(s) 460
BshVI ATCGAT 1 cut(s) 1095
BsiSI CCGG 3 cut(s) 515, 830, 1583
BsmAI GTCTC 3 cut(s) 73, 79, 1466
BsmI GAATGC 1 cut(s) 683
BsnI GGCC 4 cut(s) 774, 1242, 1284, 1350
Bsp1286I GDGCHC 1 cut(s) 1034
Bsp13I TCCGGA 1 cut(s) 829
Bsp143I GATC 7 cut(s) 304, 832, 1012, 1092, 1096, 1579, 1606
Bsp19I CCATGG 1 cut(s) 1035
BspANI GGCC 4 cut(s) 774, 1242, 1284, 1350
BspCNI CTCAG 5 cut(s) 229, 457, 804, 1216, 1383
BspDI ATCGAT 1 cut(s) 1095
BspEI TCCGGA 1 cut(s) 829
BspLI GGNNCC 2 cut(s) 462, 1033
BspMAI CTGCAG 1 cut(s) 1528
BspPI GGATC 3 cut(s) 840, 1574, 1601
BspT107I GGYRCC 1 cut(s) 460
BsrI ACTGG 1 cut(s) 775
BssECI CCNNGG 2 cut(s) 1035, 1450
BssMI GATC 7 cut(s) 304, 832, 1012, 1092, 1096, 1579, 1606
BssSI CACGAG 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 1035
Bst2BI CACGAG 1 cut(s) 474
Bst4CI ACNGT 6 cut(s) 572, 611, 653, 974, 1153, 1469
Bst6I CTCTTC 1 cut(s) 698
BstC8I GCNNGC 2 cut(s) 1082, 1244
BstDEI CTNAG 6 cut(s) 216, 465, 812, 1224, 1325, 1391
BstDSI CCRYGG 1 cut(s) 1035
BstF5I GGATG 6 cut(s) 157, 523, 808, 885, 970, 1418
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 7 cut(s) 307, 835, 1015, 1095, 1099, 1582, 1609
BstMAI GTCTC 3 cut(s) 73, 79, 1466
BstMBI GATC 7 cut(s) 304, 832, 1012, 1092, 1096, 1579, 1606
BstMWI GCNNNNNNNGC 2 cut(s) 15, 771
BstSFI CTRYAG 1 cut(s) 1524
BstV1I GCAGC 6 cut(s) 21, 24, 27, 457, 562, 1374
BstV2I GAAGAC 2 cut(s) 392, 503
BstX2I RGATCY 3 cut(s) 832, 1579, 1606
BstXI CCANNNNNNTGG 1 cut(s) 901
BstYI RGATCY 3 cut(s) 832, 1579, 1606
Bsu15I ATCGAT 1 cut(s) 1095
Bsu36I CCTNAGG 1 cut(s) 465
BsuI GTATCC 1 cut(s) 658
BsuRI GGCC 4 cut(s) 774, 1242, 1284, 1350
BsuTUI ATCGAT 1 cut(s) 1095
BtgI CCRYGG 1 cut(s) 1035
BtsCI GGATG 6 cut(s) 157, 523, 808, 885, 970, 1418
BtsIMutI CAGTG 3 cut(s) 577, 782, 1474
Cac8I GCNNGC 2 cut(s) 1082, 1244
CfoI GCGC 1 cut(s) 9
ClaI ATCGAT 1 cut(s) 1095
CseI GACGC 1 cut(s) 1115
Csp6I GTAC 1 cut(s) 1431
CspCI CAANNNNNGTGG 2 cut(s) 1153, 1188
CviAII CATG 7 cut(s) 61, 101, 556, 1036, 1280, 1361, 1479
CviQI GTAC 1 cut(s) 1431
DdeI CTNAG 6 cut(s) 216, 465, 812, 1224, 1325, 1391
DpnI GATC 7 cut(s) 306, 834, 1014, 1094, 1098, 1581, 1608
DpnII GATC 7 cut(s) 304, 832, 1012, 1092, 1096, 1579, 1606
EaeI YGGCCR 1 cut(s) 1240
Eam1104I CTCTTC 1 cut(s) 698
EarI CTCTTC 1 cut(s) 698
Eco130I CCWWGG 1 cut(s) 1035
Eco147I AGGCCT 1 cut(s) 1350
Eco24I GRGCYC 1 cut(s) 1034
Eco81I CCTNAGG 1 cut(s) 465
EcoT14I CCWWGG 1 cut(s) 1035
EcoT22I ATGCAT 1 cut(s) 1534
EcoT38I GRGCYC 1 cut(s) 1034
ErhI CCWWGG 1 cut(s) 1035
FaeI CATG 7 cut(s) 64, 104, 559, 1039, 1283, 1364, 1482
FalI AAGNNNNNCTT 2 cut(s) 1491, 1523
FatI CATG 7 cut(s) 60, 100, 555, 1035, 1279, 1360, 1478
FbaI TGATCA 1 cut(s) 304
Fnu4HI GCNGC 6 cut(s) 10, 13, 16, 471, 576, 1388
FokI GGATG 6 cut(s) 164, 530, 815, 892, 977, 1425
FriOI GRGCYC 1 cut(s) 1034
Fsp4HI GCNGC 6 cut(s) 10, 13, 16, 471, 576, 1388
FspBI CTAG 2 cut(s) 770, 1232
FspI TGCGCA 1 cut(s) 8
GlaI GCGC 1 cut(s) 8
GluI GCNGC 6 cut(s) 10, 13, 16, 471, 576, 1388
GsuI CTGGAG 1 cut(s) 1404
HaeIII GGCC 4 cut(s) 774, 1242, 1284, 1350
HapII CCGG 3 cut(s) 515, 830, 1583
HgaI GACGC 1 cut(s) 1115
HhaI GCGC 1 cut(s) 9
Hin1II CATG 7 cut(s) 64, 104, 559, 1039, 1283, 1364, 1482
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HincII GTYRAC 2 cut(s) 49, 230
HindII GTYRAC 2 cut(s) 49, 230
HinfI GANTC 4 cut(s) 349, 454, 759, 916
HpaII CCGG 3 cut(s) 515, 830, 1583
HphI GGTGA 1 cut(s) 505
Hpy166II GTNNAC 3 cut(s) 49, 230, 525
Hpy188I TCNGA 2 cut(s) 1225, 1451
Hpy8I GTNNAC 3 cut(s) 49, 230, 525
Hpy99I CGWCG 1 cut(s) 1131
HpyAV CCTTC 4 cut(s) 430, 869, 1403, 1561
HpyCH4III ACNGT 6 cut(s) 572, 611, 653, 974, 1153, 1469
HpyCH4V TGCA 7 cut(s) 473, 555, 765, 1080, 1185, 1526, 1532
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 771
HpyF3I CTNAG 6 cut(s) 216, 465, 812, 1224, 1325, 1391
Hsp92II CATG 7 cut(s) 64, 104, 559, 1039, 1283, 1364, 1482
HspAI GCGC 1 cut(s) 7
Kpn2I TCCGGA 1 cut(s) 829
Ksp22I TGATCA 1 cut(s) 304
Kzo9I GATC 7 cut(s) 304, 832, 1012, 1092, 1096, 1579, 1606
LmnI GCTCC 2 cut(s) 727, 1037
Lsp1109I GCAGC 6 cut(s) 21, 24, 27, 457, 562, 1374
LweI GCATC 4 cut(s) 142, 1218, 1519, 1541
MaeI CTAG 2 cut(s) 770, 1232
MaeIII GTNAC 6 cut(s) 585, 733, 943, 968, 1153, 1289
MalI GATC 7 cut(s) 306, 834, 1014, 1094, 1098, 1581, 1608
MboI GATC 7 cut(s) 304, 832, 1012, 1092, 1096, 1579, 1606
MboII GAAGA 6 cut(s) 27, 379, 392, 508, 614, 685
MflI RGATCY 3 cut(s) 832, 1579, 1606
MhlI GDGCHC 1 cut(s) 1034
MlsI TGGCCA 1 cut(s) 1242
MluCI AATT 8 cut(s) 239, 294, 392, 672, 1197, 1319, 1371, 1544
MluNI TGGCCA 1 cut(s) 1242
MlyI GAGTC 1 cut(s) 768
MmeI TCCRAC 1 cut(s) 780
Mox20I TGGCCA 1 cut(s) 1242
Mph1103I ATGCAT 1 cut(s) 1534
MroI TCCGGA 1 cut(s) 829
MroXI GAANNNNTTC 3 cut(s) 164, 177, 1140
MscI TGGCCA 1 cut(s) 1242
MseI TTAA 4 cut(s) 1041, 1367, 1460, 1601
Msp20I TGGCCA 1 cut(s) 1242
MspI CCGG 3 cut(s) 515, 830, 1583
Mva1269I GAATGC 1 cut(s) 683
MwoI GCNNNNNNNGC 2 cut(s) 15, 771
NcoI CCATGG 1 cut(s) 1035
NdeII GATC 7 cut(s) 304, 832, 1012, 1092, 1096, 1579, 1606
NlaIII CATG 7 cut(s) 64, 104, 559, 1039, 1283, 1364, 1482
NlaIV GGNNCC 2 cut(s) 462, 1033
NsbI TGCGCA 1 cut(s) 8
NsiI ATGCAT 1 cut(s) 1534
PceI AGGCCT 1 cut(s) 1350
PctI GAATGC 1 cut(s) 683
PdmI GAANNNNTTC 3 cut(s) 164, 177, 1140
PfeI GAWTC 3 cut(s) 349, 454, 916
PflFI GACNNNGTC 1 cut(s) 758
PkrI GCNGC 6 cut(s) 11, 14, 17, 472, 577, 1389
PleI GAGTC 1 cut(s) 767
PpsI GAGTC 1 cut(s) 767
PspN4I GGNNCC 2 cut(s) 462, 1033
PstI CTGCAG 1 cut(s) 1528
PsuI RGATCY 3 cut(s) 832, 1579, 1606
PsyI GACNNNGTC 1 cut(s) 758
RsaI GTAC 1 cut(s) 1432
RsaNI GTAC 1 cut(s) 1431
SaqAI TTAA 4 cut(s) 1041, 1367, 1460, 1601
SatI GCNGC 6 cut(s) 10, 13, 16, 471, 576, 1388
Sau3AI GATC 7 cut(s) 304, 832, 1012, 1092, 1096, 1579, 1606
SchI GAGTC 1 cut(s) 768
SduI GDGCHC 1 cut(s) 1034
SfaNI GCATC 4 cut(s) 142, 1218, 1519, 1541
SfcI CTRYAG 1 cut(s) 1524
SmlI CTYRAG 3 cut(s) 19, 449, 1508
SmoI CTYRAG 3 cut(s) 19, 449, 1508
Sse9I AATT 8 cut(s) 239, 294, 392, 672, 1197, 1319, 1371, 1544
SseBI AGGCCT 1 cut(s) 1350
SspMI CTAG 2 cut(s) 770, 1232
StuI AGGCCT 1 cut(s) 1350
StyI CCWWGG 1 cut(s) 1035
TaaI ACNGT 6 cut(s) 572, 611, 653, 974, 1153, 1469
TaqI TCGA 4 cut(s) 77, 648, 1095, 1565
TasI AATT 8 cut(s) 239, 294, 392, 672, 1197, 1319, 1371, 1544
TfiI GAWTC 3 cut(s) 349, 454, 916
Tru1I TTAA 4 cut(s) 1041, 1367, 1460, 1601
Tru9I TTAA 4 cut(s) 1041, 1367, 1460, 1601
TscAI CASTG 3 cut(s) 577, 782, 1474
TseI GCWGC 6 cut(s) 9, 12, 15, 470, 575, 1387
TspDTI ATGAA 5 cut(s) 62, 693, 819, 1268, 1502
TspGWI ACGGA 4 cut(s) 34, 158, 771, 1086
TspRI CASTG 3 cut(s) 577, 782, 1474
Tth111I GACNNNGTC 1 cut(s) 758
XapI RAATTY 1 cut(s) 1197
XmnI GAANNNNTTC 3 cut(s) 164, 177, 1140
XspI CTAG 2 cut(s) 770, 1232
Zsp2I ATGCAT 1 cut(s) 1534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.