Belongs to the CRISP family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 377 |
| AccB1I | GGYRCC | 1 cut(s) | 377 |
| AccBSI | CCGCTC | 2 cut(s) | 113, 119 |
| AciI | CCGC | 4 cut(s) | 111, 119, 207, 230 |
| AclWI | GGATC | 3 cut(s) | 143, 156, 395 |
| AcoI | YGGCCR | 2 cut(s) | 304, 421 |
| AfaI | GTAC | 1 cut(s) | 379 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 144, 360, 387 |
| AflIII | ACRYGT | 1 cut(s) | 213 |
| AluBI | AGCT | 3 cut(s) | 164, 227, 338 |
| AluI | AGCT | 3 cut(s) | 164, 227, 338 |
| Alw21I | GWGCWC | 1 cut(s) | 289 |
| Alw26I | GTCTC | 1 cut(s) | 41 |
| AlwI | GGATC | 3 cut(s) | 143, 156, 395 |
| Ama87I | CYCGRG | 2 cut(s) | 114, 406 |
| AoxI | GGCC | 3 cut(s) | 255, 304, 421 |
| ApeKI | GCWGC | 3 cut(s) | 161, 204, 227 |
| Asp718I | GGTACC | 1 cut(s) | 377 |
| AspLEI | GCGC | 1 cut(s) | 330 |
| AsuC2I | CCSGG | 2 cut(s) | 407, 408 |
| AsuHPI | GGTGA | 3 cut(s) | 72, 221, 244 |
| AvaI | CYCGRG | 2 cut(s) | 114, 406 |
| BalI | TGGCCA | 2 cut(s) | 306, 423 |
| BamHI | GGATCC | 1 cut(s) | 148 |
| BanI | GGYRCC | 1 cut(s) | 377 |
| BauI | CACGAG | 1 cut(s) | 339 |
| Bbv12I | GWGCWC | 1 cut(s) | 289 |
| BbvI | GCAGC | 3 cut(s) | 173, 214, 216 |
| BcnI | CCSGG | 2 cut(s) | 407, 408 |
| BcoDI | GTCTC | 1 cut(s) | 41 |
| BfaI | CTAG | 1 cut(s) | 158 |
| BisI | GCNGC | 5 cut(s) | 111, 120, 162, 205, 228 |
| BlsI | GCNGC | 5 cut(s) | 112, 121, 163, 206, 229 |
| Bme1390I | CCNGG | 2 cut(s) | 407, 408 |
| BmeT110I | CYCGRG | 2 cut(s) | 114, 406 |
| BmiI | GGNNCC | 2 cut(s) | 150, 379 |
| BmrFI | CCNGG | 2 cut(s) | 407, 408 |
| BpuMI | CCSGG | 2 cut(s) | 407, 408 |
| BsaJI | CCNNGG | 2 cut(s) | 405, 406 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 271, 301 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 144, 360, 387 |
| BseDI | CCNNGG | 2 cut(s) | 405, 406 |
| BseGI | GGATG | 1 cut(s) | 353 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 144, 360, 387 |
| BseMII | CTCAG | 2 cut(s) | 329, 348 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseXI | GCAGC | 3 cut(s) | 173, 214, 216 |
| BshFI | GGCC | 3 cut(s) | 257, 306, 423 |
| BshNI | GGYRCC | 1 cut(s) | 377 |
| BsiHKAI | GWGCWC | 1 cut(s) | 289 |
| BsiHKCI | CYCGRG | 2 cut(s) | 114, 406 |
| BsiSI | CCGG | 2 cut(s) | 219, 407 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 144, 360, 387 |
| BsmAI | GTCTC | 1 cut(s) | 41 |
| BsnI | GGCC | 3 cut(s) | 257, 306, 423 |
| BsoBI | CYCGRG | 2 cut(s) | 114, 406 |
| Bsp1286I | GDGCHC | 1 cut(s) | 289 |
| Bsp143I | GATC | 2 cut(s) | 148, 400 |
| BspACI | CCGC | 4 cut(s) | 111, 119, 207, 230 |
| BspANI | GGCC | 3 cut(s) | 257, 306, 423 |
| BspCNI | CTCAG | 2 cut(s) | 328, 347 |
| BspLI | GGNNCC | 2 cut(s) | 150, 379 |
| BspPI | GGATC | 3 cut(s) | 143, 156, 395 |
| BspT107I | GGYRCC | 1 cut(s) | 377 |
| BsrBI | CCGCTC | 2 cut(s) | 113, 119 |
| BssECI | CCNNGG | 2 cut(s) | 405, 406 |
| BssMI | GATC | 2 cut(s) | 148, 400 |
| BssSI | CACGAG | 1 cut(s) | 339 |
| Bst2BI | CACGAG | 1 cut(s) | 339 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 167 |
| BstDEI | CTNAG | 2 cut(s) | 315, 334 |
| BstF5I | GGATG | 1 cut(s) | 353 |
| BstHHI | GCGC | 1 cut(s) | 330 |
| BstKTI | GATC | 2 cut(s) | 151, 403 |
| BstMAI | GTCTC | 1 cut(s) | 41 |
| BstMBI | GATC | 2 cut(s) | 148, 400 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 107, 116, 119, 167 |
| BstNSI | RCATGY | 1 cut(s) | 217 |
| BstSCI | CCNGG | 2 cut(s) | 405, 406 |
| BstV1I | GCAGC | 3 cut(s) | 173, 214, 216 |
| BstX2I | RGATCY | 1 cut(s) | 148 |
| BstYI | RGATCY | 1 cut(s) | 148 |
| BsuRI | GGCC | 3 cut(s) | 257, 306, 423 |
| BtsCI | GGATG | 1 cut(s) | 353 |
| CfoI | GCGC | 1 cut(s) | 330 |
| Cfr9I | CCCGGG | 1 cut(s) | 406 |
| Csp6I | GTAC | 1 cut(s) | 378 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 283, 318 |
| CviAII | CATG | 1 cut(s) | 214 |
| CviJI | RGCY | 8 cut(s) | 137, 164, 227, 257, 306, 338, 423, 430 |
| CviKI_1 | RGCY | 8 cut(s) | 137, 164, 227, 257, 306, 338, 423, 430 |
| CviQI | GTAC | 1 cut(s) | 378 |
| DdeI | CTNAG | 2 cut(s) | 315, 334 |
| DpnI | GATC | 2 cut(s) | 150, 402 |
| DpnII | GATC | 2 cut(s) | 148, 400 |
| EaeI | YGGCCR | 2 cut(s) | 304, 421 |
| Eco88I | CYCGRG | 2 cut(s) | 114, 406 |
| FaeI | CATG | 1 cut(s) | 217 |
| FaiI | YATR | 7 cut(s) | 168, 180, 215, 264, 312, 399, 417 |
| FatI | CATG | 1 cut(s) | 213 |
| Fnu4HI | GCNGC | 5 cut(s) | 111, 120, 162, 205, 228 |
| FokI | GGATG | 1 cut(s) | 360 |
| Fsp4HI | GCNGC | 5 cut(s) | 111, 120, 162, 205, 228 |
| FspBI | CTAG | 1 cut(s) | 158 |
| GlaI | GCGC | 1 cut(s) | 329 |
| GluI | GCNGC | 5 cut(s) | 111, 120, 162, 205, 228 |
| HaeIII | GGCC | 3 cut(s) | 257, 306, 423 |
| HapII | CCGG | 2 cut(s) | 219, 407 |
| HhaI | GCGC | 1 cut(s) | 330 |
| Hin1II | CATG | 1 cut(s) | 217 |
| Hin6I | GCGC | 1 cut(s) | 328 |
| HinP1I | GCGC | 1 cut(s) | 328 |
| HinfI | GANTC | 1 cut(s) | 77 |
| HpaII | CCGG | 2 cut(s) | 219, 407 |
| HphI | GGTGA | 3 cut(s) | 72, 221, 244 |
| Hpy166II | GTNNAC | 1 cut(s) | 235 |
| Hpy188I | TCNGA | 1 cut(s) | 250 |
| Hpy8I | GTNNAC | 1 cut(s) | 235 |
| HpyAV | CCTTC | 2 cut(s) | 247, 391 |
| HpyCH4IV | ACGT | 1 cut(s) | 143 |
| HpyCH4V | TGCA | 3 cut(s) | 170, 366, 393 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 107, 116, 119, 167 |
| HpyF3I | CTNAG | 2 cut(s) | 315, 334 |
| HpySE526I | ACGT | 1 cut(s) | 143 |
| Hsp92II | CATG | 1 cut(s) | 217 |
| HspAI | GCGC | 1 cut(s) | 328 |
| KpnI | GGTACC | 1 cut(s) | 381 |
| Kzo9I | GATC | 2 cut(s) | 148, 400 |
| LmnI | GCTCC | 1 cut(s) | 292 |
| LpnPI | CCDG | 4 cut(s) | 232, 295, 302, 420 |
| Lsp1109I | GCAGC | 3 cut(s) | 173, 214, 216 |
| MaeI | CTAG | 1 cut(s) | 158 |
| MaeII | ACGT | 1 cut(s) | 143 |
| MaeIII | GTNAC | 1 cut(s) | 209 |
| MalI | GATC | 2 cut(s) | 150, 402 |
| MbiI | CCGCTC | 2 cut(s) | 113, 119 |
| MboI | GATC | 2 cut(s) | 148, 400 |
| MflI | RGATCY | 1 cut(s) | 148 |
| MhlI | GDGCHC | 1 cut(s) | 289 |
| MlsI | TGGCCA | 2 cut(s) | 306, 423 |
| MluCI | AATT | 2 cut(s) | 184, 394 |
| MluNI | TGGCCA | 2 cut(s) | 306, 423 |
| MlyI | GAGTC | 1 cut(s) | 71 |
| MmeI | TCCRAC | 1 cut(s) | 82 |
| MnlI | CCTC | 4 cut(s) | 62, 65, 104, 368 |
| Mox20I | TGGCCA | 2 cut(s) | 306, 423 |
| MscI | TGGCCA | 2 cut(s) | 306, 423 |
| MseI | TTAA | 1 cut(s) | 140 |
| Msp20I | TGGCCA | 2 cut(s) | 306, 423 |
| MspA1I | CMGCKG | 2 cut(s) | 207, 227 |
| MspI | CCGG | 2 cut(s) | 219, 407 |
| MspR9I | CCNGG | 2 cut(s) | 407, 408 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 107, 116, 119, 167 |
| NciI | CCSGG | 2 cut(s) | 407, 408 |
| NdeII | GATC | 2 cut(s) | 148, 400 |
| NlaIII | CATG | 1 cut(s) | 217 |
| NlaIV | GGNNCC | 2 cut(s) | 150, 379 |
| NmuCI | GTSAC | 1 cut(s) | 209 |
| NspI | RCATGY | 1 cut(s) | 217 |
| PaeR7I | CTCGAG | 1 cut(s) | 114 |
| PciI | ACATGT | 1 cut(s) | 213 |
| PkrI | GCNGC | 5 cut(s) | 112, 121, 163, 206, 229 |
| PleI | GAGTC | 1 cut(s) | 71 |
| PpsI | GAGTC | 1 cut(s) | 71 |
| PscI | ACATGT | 1 cut(s) | 213 |
| PspN4I | GGNNCC | 2 cut(s) | 150, 379 |
| PspXI | VCTCGAGB | 1 cut(s) | 114 |
| PsuI | RGATCY | 1 cut(s) | 148 |
| PvuII | CAGCTG | 1 cut(s) | 227 |
| RsaI | GTAC | 1 cut(s) | 379 |
| RsaNI | GTAC | 1 cut(s) | 378 |
| SaqAI | TTAA | 1 cut(s) | 140 |
| SatI | GCNGC | 5 cut(s) | 111, 120, 162, 205, 228 |
| Sau3AI | GATC | 2 cut(s) | 148, 400 |
| SchI | GAGTC | 1 cut(s) | 71 |
| ScrFI | CCNGG | 2 cut(s) | 407, 408 |
| SduI | GDGCHC | 1 cut(s) | 289 |
| Sfr274I | CTCGAG | 1 cut(s) | 114 |
| SlaI | CTCGAG | 1 cut(s) | 114 |
| SmaI | CCCGGG | 1 cut(s) | 408 |
| SmlI | CTYRAG | 1 cut(s) | 114 |
| SmoI | CTYRAG | 1 cut(s) | 114 |
| Sse9I | AATT | 2 cut(s) | 184, 394 |
| SsiI | CCGC | 4 cut(s) | 111, 119, 207, 230 |
| SspMI | CTAG | 1 cut(s) | 158 |
| StyD4I | CCNGG | 2 cut(s) | 405, 406 |
| TaiI | ACGT | 1 cut(s) | 146 |
| TaqI | TCGA | 2 cut(s) | 115, 133 |
| TasI | AATT | 2 cut(s) | 184, 394 |
| TauI | GCSGC | 2 cut(s) | 113, 122 |
| Tru1I | TTAA | 1 cut(s) | 140 |
| Tru9I | TTAA | 1 cut(s) | 140 |
| TseFI | GTSAC | 1 cut(s) | 209 |
| TseI | GCWGC | 3 cut(s) | 161, 204, 227 |
| Tsp45I | GTSAC | 1 cut(s) | 209 |
| TspDTI | ATGAA | 1 cut(s) | 373 |
| TspMI | CCCGGG | 1 cut(s) | 406 |
| XceI | RCATGY | 1 cut(s) | 217 |
| XhoI | CTCGAG | 1 cut(s) | 114 |
| XmaI | CCCGGG | 1 cut(s) | 406 |
| XspI | CTAG | 1 cut(s) | 158 |