Belongs to the CRISP family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 29 |
| AciI | CCGC | 3 cut(s) | 27, 29, 148 |
| AcsI | RAATTY | 1 cut(s) | 83 |
| AcuI | CTGAAG | 1 cut(s) | 126 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 33, 98 |
| AgsI | TTSAA | 1 cut(s) | 260 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 23, 55 |
| AluBI | AGCT | 3 cut(s) | 50, 104, 123 |
| AluI | AGCT | 3 cut(s) | 50, 104, 123 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 138 |
| ApeKI | GCWGC | 1 cut(s) | 132 |
| ApoI | RAATTY | 1 cut(s) | 83 |
| AspS9I | GGNCC | 1 cut(s) | 150 |
| AsuII | TTCGAA | 1 cut(s) | 53 |
| AvaII | GGWCC | 1 cut(s) | 150 |
| BbsI | GAAGAC | 1 cut(s) | 75 |
| BbvI | GCAGC | 1 cut(s) | 144 |
| BclI | TGATCA | 1 cut(s) | 13 |
| BisI | GCNGC | 2 cut(s) | 27, 133 |
| BlsI | GCNGC | 2 cut(s) | 28, 134 |
| Bme18I | GGWCC | 1 cut(s) | 150 |
| BmgT120I | GGNCC | 1 cut(s) | 150 |
| BmiI | GGNNCC | 1 cut(s) | 41 |
| BmsI | GCATC | 2 cut(s) | 144, 160 |
| BpiI | GAAGAC | 1 cut(s) | 75 |
| Bpu14I | TTCGAA | 1 cut(s) | 53 |
| BsaJI | CCNNGG | 3 cut(s) | 27, 60, 153 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 33, 98 |
| BseDI | CCNNGG | 3 cut(s) | 27, 60, 153 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 33, 98 |
| BseXI | GCAGC | 1 cut(s) | 144 |
| BseYI | CCCAGC | 1 cut(s) | 144 |
| Bsh1236I | CGCG | 1 cut(s) | 29 |
| BslFI | GGGAC | 1 cut(s) | 172 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 33, 98 |
| BsmFI | GGGAC | 1 cut(s) | 172 |
| BsmI | GAATGC | 1 cut(s) | 61 |
| Bsp119I | TTCGAA | 1 cut(s) | 53 |
| Bsp143I | GATC | 1 cut(s) | 13 |
| BspACI | CCGC | 3 cut(s) | 27, 29, 148 |
| BspFNI | CGCG | 1 cut(s) | 29 |
| BspLI | GGNNCC | 1 cut(s) | 41 |
| BspQI | GCTCTTC | 1 cut(s) | 215 |
| BspT104I | TTCGAA | 1 cut(s) | 53 |
| BssECI | CCNNGG | 3 cut(s) | 27, 60, 153 |
| BssMI | GATC | 1 cut(s) | 13 |
| BssT1I | CCWWGG | 2 cut(s) | 60, 153 |
| Bst4CI | ACNGT | 1 cut(s) | 23 |
| Bst6I | CTCTTC | 1 cut(s) | 215 |
| BstBI | TTCGAA | 1 cut(s) | 53 |
| BstC8I | GCNNGC | 1 cut(s) | 227 |
| BstDSI | CCRYGG | 1 cut(s) | 27 |
| BstFNI | CGCG | 1 cut(s) | 29 |
| BstKTI | GATC | 1 cut(s) | 16 |
| BstMBI | GATC | 1 cut(s) | 13 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 56, 129, 170 |
| BstUI | CGCG | 1 cut(s) | 29 |
| BstV1I | GCAGC | 1 cut(s) | 144 |
| BstV2I | GAAGAC | 1 cut(s) | 75 |
| BtgI | CCRYGG | 1 cut(s) | 27 |
| BtsIMutI | CAGTG | 1 cut(s) | 28 |
| Cac8I | GCNNGC | 1 cut(s) | 227 |
| CaiI | CAGNNNCTG | 1 cut(s) | 138 |
| Cfr13I | GGNCC | 1 cut(s) | 150 |
| Cfr42I | CCGCGG | 1 cut(s) | 30 |
| CviJI | RGCY | 3 cut(s) | 50, 104, 123 |
| CviKI_1 | RGCY | 3 cut(s) | 50, 104, 123 |
| DpnI | GATC | 1 cut(s) | 15 |
| DpnII | GATC | 1 cut(s) | 13 |
| Eam1104I | CTCTTC | 1 cut(s) | 215 |
| EarI | CTCTTC | 1 cut(s) | 215 |
| Eco130I | CCWWGG | 2 cut(s) | 60, 153 |
| Eco47I | GGWCC | 1 cut(s) | 150 |
| Eco57I | CTGAAG | 1 cut(s) | 126 |
| EcoT14I | CCWWGG | 2 cut(s) | 60, 153 |
| ErhI | CCWWGG | 2 cut(s) | 60, 153 |
| FaqI | GGGAC | 1 cut(s) | 172 |
| FbaI | TGATCA | 1 cut(s) | 13 |
| Fnu4HI | GCNGC | 2 cut(s) | 27, 133 |
| Fsp4HI | GCNGC | 2 cut(s) | 27, 133 |
| GluI | GCNGC | 2 cut(s) | 27, 133 |
| GsaI | CCCAGC | 1 cut(s) | 148 |
| Hpy188I | TCNGA | 1 cut(s) | 178 |
| Hpy188III | TCNNGA | 1 cut(s) | 167 |
| HpyAV | CCTTC | 1 cut(s) | 104 |
| HpyCH4III | ACNGT | 1 cut(s) | 23 |
| HpyCH4V | TGCA | 1 cut(s) | 80 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 56, 129, 170 |
| Ksp22I | TGATCA | 1 cut(s) | 13 |
| KspI | CCGCGG | 1 cut(s) | 30 |
| Kzo9I | GATC | 1 cut(s) | 13 |
| LguI | GCTCTTC | 1 cut(s) | 215 |
| LmnI | GCTCC | 1 cut(s) | 101 |
| LpnPI | CCDG | 1 cut(s) | 158 |
| Lsp1109I | GCAGC | 1 cut(s) | 144 |
| LweI | GCATC | 2 cut(s) | 144, 160 |
| MalI | GATC | 1 cut(s) | 15 |
| MboI | GATC | 1 cut(s) | 13 |
| MboII | GAAGA | 5 cut(s) | 77, 80, 119, 210, 232 |
| MluCI | AATT | 1 cut(s) | 83 |
| MnlI | CCTC | 3 cut(s) | 30, 91, 248 |
| MspA1I | CMGCKG | 2 cut(s) | 29, 148 |
| Mva1269I | GAATGC | 1 cut(s) | 61 |
| MvnI | CGCG | 1 cut(s) | 29 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 56, 129, 170 |
| NdeII | GATC | 1 cut(s) | 13 |
| NlaIV | GGNNCC | 1 cut(s) | 41 |
| NspV | TTCGAA | 1 cut(s) | 53 |
| PciSI | GCTCTTC | 1 cut(s) | 215 |
| PctI | GAATGC | 1 cut(s) | 61 |
| PkrI | GCNGC | 2 cut(s) | 28, 134 |
| PspFI | CCCAGC | 1 cut(s) | 144 |
| PspN4I | GGNNCC | 1 cut(s) | 41 |
| PspPI | GGNCC | 1 cut(s) | 150 |
| PstNI | CAGNNNCTG | 1 cut(s) | 138 |
| SacII | CCGCGG | 1 cut(s) | 30 |
| SapI | GCTCTTC | 1 cut(s) | 215 |
| SatI | GCNGC | 2 cut(s) | 27, 133 |
| Sau3AI | GATC | 1 cut(s) | 13 |
| Sau96I | GGNCC | 1 cut(s) | 150 |
| SetI | ASST | 6 cut(s) | 41, 52, 106, 121, 125, 250 |
| SfaNI | GCATC | 2 cut(s) | 144, 160 |
| Sfr303I | CCGCGG | 1 cut(s) | 30 |
| SfuI | TTCGAA | 1 cut(s) | 53 |
| SgrBI | CCGCGG | 1 cut(s) | 30 |
| SinI | GGWCC | 1 cut(s) | 150 |
| Sse9I | AATT | 1 cut(s) | 83 |
| SsiI | CCGC | 3 cut(s) | 27, 29, 148 |
| StyI | CCWWGG | 2 cut(s) | 60, 153 |
| TaaI | ACNGT | 1 cut(s) | 23 |
| TaqI | TCGA | 1 cut(s) | 53 |
| TasI | AATT | 1 cut(s) | 83 |
| TauI | GCSGC | 1 cut(s) | 29 |
| TscAI | CASTG | 1 cut(s) | 28 |
| TseI | GCWGC | 1 cut(s) | 132 |
| TspRI | CASTG | 1 cut(s) | 28 |
| VpaK11BI | GGWCC | 1 cut(s) | 150 |
| XapI | RAATTY | 1 cut(s) | 83 |