Rw6G011470

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001302
Physical Location & Seq
Forward (+)
20196773 .. 20197270
498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G011470.1

Sequence Viewer

Length: 498 bp
ATGAAATCGTCTACAGCTAAGATTTCTTTCGCTCTCATTTCCCTCACAGCCATAGCCCTACTCGATTCGATTCATGCCCAAGACTCACCGCAAGACTTTCTTGATGCCCACAATGCTGCTAGAGCCGAGGTGGGTGTGGCGCCCTTGACTTGGGACGACCAAGTAGCACGTTTTGCACAAGACTATGCCAATACTCATGTAGGCGACTGCGAACTCGTCCACTCTACCGATATGCGATACGGTGAAAACCTTGCAATGAACCCCGCCATTGACATGTCGGGCACAGAAGCAGTCAACCTGTGGGTGGGAGAGAAGCCCAACTACGATTACAACTCGAACTCTTGCGCCCCCGGCCAGGTGTGTGGACATTATACGCAAGTCGTTTGGCGTAACTCGTTGCGAGTTGGGTGTGCCAAAGTGAGGTGCAACAGTGGAGGTACCTTCATTGGGTGCAACTATGACCCCCCGGGAAACTGGGATGGGGAGAAACCTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

165

Amino Acids

17.96

Weight (kDa)

4.88

Isoelectric Point (pI)

30.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 34 - 153 7.4e-20 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 437
AccB1I GGYRCC 2 cut(s) 139, 437
AccI GTMKAC 1 cut(s) 11
AciI CCGC 2 cut(s) 89, 264
AcoI YGGCCR 1 cut(s) 352
AcyI GRCGYC 1 cut(s) 140
AfaI GTAC 1 cut(s) 439
AfiI CCNNNNNNNGG 5 cut(s) 150, 304, 355, 420, 447
AflIII ACRYGT 1 cut(s) 273
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 1 cut(s) 17
AluI AGCT 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 466
AoxI GGCC 1 cut(s) 352
ApeKI GCWGC 1 cut(s) 116
Asp718I GGTACC 1 cut(s) 437
AspLEI GCGC 2 cut(s) 142, 347
AsuC2I CCSGG 3 cut(s) 351, 467, 468
AsuHPI GGTGA 2 cut(s) 78, 254
AvaI CYCGRG 1 cut(s) 466
BaeGI GKGCMC 1 cut(s) 284
BanI GGYRCC 2 cut(s) 139, 437
BarI GAAGNNNNNNTAC 2 cut(s) 305, 337
BbvI GCAGC 1 cut(s) 103
BccI CCATC 1 cut(s) 473
BciT130I CCWGG 1 cut(s) 356
BcnI CCSGG 3 cut(s) 351, 467, 468
BfaI CTAG 1 cut(s) 120
BfmI CTRYAG 1 cut(s) 12
BfoI RGCGCY 1 cut(s) 143
BisI GCNGC 1 cut(s) 117
BlsI GCNGC 1 cut(s) 118
Bme1390I CCNGG 4 cut(s) 351, 356, 467, 468
BmeT110I CYCGRG 1 cut(s) 466
BmiI GGNNCC 2 cut(s) 141, 439
BmrFI CCNGG 4 cut(s) 351, 356, 467, 468
BmrI ACTGGG 1 cut(s) 484
BmsI GCATC 1 cut(s) 94
BmuI ACTGGG 1 cut(s) 484
BpuMI CCSGG 3 cut(s) 351, 467, 468
BsaHI GRCGYC 1 cut(s) 140
BsaJI CCNNGG 4 cut(s) 126, 349, 465, 466
Bsc4I CCNNNNNNNGG 5 cut(s) 150, 304, 355, 420, 447
Bse1I ACTGG 1 cut(s) 479
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 4 cut(s) 126, 349, 465, 466
BseGI GGATG 1 cut(s) 484
BseLI CCNNNNNNNGG 5 cut(s) 150, 304, 355, 420, 447
BseMI GCAATG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 479
BseSI GKGCMC 1 cut(s) 284
BseXI GCAGC 1 cut(s) 103
BshFI GGCC 1 cut(s) 354
BshNI GGYRCC 2 cut(s) 139, 437
BsiHKCI CYCGRG 1 cut(s) 466
BsiSI CCGG 2 cut(s) 351, 467
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 5 cut(s) 150, 304, 355, 420, 447
BsmFI GGGAC 1 cut(s) 167
BsnI GGCC 1 cut(s) 354
BsoBI CYCGRG 1 cut(s) 466
Bsp1286I GDGCHC 1 cut(s) 284
BspACI CCGC 2 cut(s) 89, 264
BspANI GGCC 1 cut(s) 354
BspLI GGNNCC 2 cut(s) 141, 439
BspT107I GGYRCC 2 cut(s) 139, 437
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 1 cut(s) 479
BssECI CCNNGG 4 cut(s) 126, 349, 465, 466
BssNI GRCGYC 1 cut(s) 140
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 2 cut(s) 242, 431
BstACI GRCGYC 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 173
BstDEI CTNAG 1 cut(s) 18
BstF5I GGATG 1 cut(s) 484
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 2 cut(s) 142, 347
BstMWI GCNNNNNNNGC 4 cut(s) 113, 122, 173, 351
BstNI CCWGG 1 cut(s) 356
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 4 cut(s) 349, 354, 465, 466
BstSFI CTRYAG 1 cut(s) 12
BstSLI GKGCMC 1 cut(s) 284
BstV1I GCAGC 1 cut(s) 103
BstXI CCANNNNNNTGG 1 cut(s) 362
BsuRI GGCC 1 cut(s) 354
BtsCI GGATG 1 cut(s) 484
BtsIMutI CAGTG 1 cut(s) 436
CfoI GCGC 2 cut(s) 142, 347
Cfr9I CCCGGG 1 cut(s) 466
Csp6I GTAC 1 cut(s) 438
CviAII CATG 3 cut(s) 74, 197, 274
CviJI RGCY 6 cut(s) 17, 50, 56, 125, 316, 354
CviKI_1 RGCY 6 cut(s) 17, 50, 56, 125, 316, 354
CviQI GTAC 1 cut(s) 438
DdeI CTNAG 1 cut(s) 18
DinI GGCGCC 1 cut(s) 141
EaeI YGGCCR 1 cut(s) 352
Eco88I CYCGRG 1 cut(s) 466
EcoRII CCWGG 1 cut(s) 354
EgeI GGCGCC 1 cut(s) 141
EheI GGCGCC 1 cut(s) 141
FaeI CATG 3 cut(s) 77, 200, 277
FaiI YATR 8 cut(s) 53, 75, 186, 198, 233, 275, 372, 459
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FaqI GGGAC 1 cut(s) 167
FatI CATG 3 cut(s) 73, 196, 273
FauI CCCGC 1 cut(s) 271
FblI GTMKAC 1 cut(s) 11
Fnu4HI GCNGC 1 cut(s) 117
FokI GGATG 1 cut(s) 491
Fsp4HI GCNGC 1 cut(s) 117
FspBI CTAG 1 cut(s) 120
GlaI GCGC 2 cut(s) 141, 346
GluI GCNGC 1 cut(s) 117
HaeII RGCGCY 1 cut(s) 143
HaeIII GGCC 1 cut(s) 354
HapII CCGG 2 cut(s) 351, 467
HhaI GCGC 2 cut(s) 142, 347
Hin1I GRCGYC 1 cut(s) 140
Hin1II CATG 3 cut(s) 77, 200, 277
Hin6I GCGC 2 cut(s) 140, 345
HinP1I GCGC 2 cut(s) 140, 345
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 3 cut(s) 65, 70, 83
HpaII CCGG 2 cut(s) 351, 467
HphI GGTGA 2 cut(s) 78, 254
Hpy166II GTNNAC 4 cut(s) 12, 220, 295, 365
Hpy188III TCNNGA 1 cut(s) 101
Hpy8I GTNNAC 4 cut(s) 12, 220, 295, 365
HpyAV CCTTC 1 cut(s) 451
HpyCH4III ACNGT 2 cut(s) 242, 431
HpyCH4IV ACGT 1 cut(s) 169
HpyCH4V TGCA 4 cut(s) 176, 254, 426, 453
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 122, 173, 351
HpyF3I CTNAG 1 cut(s) 18
HpySE526I ACGT 1 cut(s) 169
Hsp92I GRCGYC 1 cut(s) 140
Hsp92II CATG 3 cut(s) 77, 200, 277
HspAI GCGC 2 cut(s) 140, 345
KasI GGCGCC 1 cut(s) 139
KpnI GGTACC 1 cut(s) 441
LpnPI CCDG 6 cut(s) 311, 341, 364, 368, 460, 480
Lsp1109I GCAGC 1 cut(s) 103
LweI GCATC 1 cut(s) 94
MaeI CTAG 1 cut(s) 120
MaeII ACGT 1 cut(s) 169
MaeIII GTNAC 1 cut(s) 389
MhlI GDGCHC 1 cut(s) 284
Mly113I GGCGCC 1 cut(s) 140
MlyI GAGTC 1 cut(s) 77
MnlI CCTC 4 cut(s) 53, 121, 414, 428
MslI CAYNNNNRTG 1 cut(s) 272
MspI CCGG 2 cut(s) 351, 467
MspR9I CCNGG 4 cut(s) 351, 356, 467, 468
MvaI CCWGG 1 cut(s) 356
MwoI GCNNNNNNNGC 4 cut(s) 113, 122, 173, 351
NarI GGCGCC 1 cut(s) 140
NciI CCSGG 3 cut(s) 351, 467, 468
NlaIII CATG 3 cut(s) 77, 200, 277
NlaIV GGNNCC 2 cut(s) 141, 439
NmeAIII GCCGAG 1 cut(s) 151
NspI RCATGY 1 cut(s) 277
PciI ACATGT 1 cut(s) 273
PfeI GAWTC 2 cut(s) 65, 70
PkrI GCNGC 1 cut(s) 118
PleI GAGTC 1 cut(s) 77
PluTI GGCGCC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 77
PscI ACATGT 1 cut(s) 273
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 141, 439
RsaI GTAC 1 cut(s) 439
RsaNI GTAC 1 cut(s) 438
RseI CAYNNNNRTG 1 cut(s) 272
SatI GCNGC 1 cut(s) 117
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 4 cut(s) 351, 356, 467, 468
SduI GDGCHC 1 cut(s) 284
SfaNI GCATC 1 cut(s) 94
SfcI CTRYAG 1 cut(s) 12
SfoI GGCGCC 1 cut(s) 141
SmaI CCCGGG 1 cut(s) 468
SmiMI CAYNNNNRTG 1 cut(s) 272
SsiI CCGC 2 cut(s) 89, 264
SspDI GGCGCC 1 cut(s) 139
SspMI CTAG 1 cut(s) 120
StyD4I CCNGG 4 cut(s) 349, 354, 465, 466
TaaI ACNGT 2 cut(s) 242, 431
TaiI ACGT 1 cut(s) 172
TaqI TCGA 3 cut(s) 63, 68, 335
TfiI GAWTC 2 cut(s) 65, 70
TscAI CASTG 1 cut(s) 436
TseI GCWGC 1 cut(s) 116
TspDTI ATGAA 4 cut(s) 17, 62, 272, 433
TspMI CCCGGG 1 cut(s) 466
TspRI CASTG 1 cut(s) 436
XceI RCATGY 1 cut(s) 277
XmaI CCCGGG 1 cut(s) 466
XmiI GTMKAC 1 cut(s) 11
XspI CTAG 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.