pycom05g10520

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001302
Physical Location & Seq
Reverse (-)
13903357 .. 13904911
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g10520.2

Sequence Viewer

Length: 585 bp
ATGACTGCTAGGTGTCAACATTTACCGTTCCCCGCTATTATAAATACTCCGCACCATAACATCTCATTTGCCCATCCACAAAGAGAACAAACCATTAACATGGGGTTGTGCAATATTTCCCTAGCTCTCCTTTTCATTTTAGGCTCAGTCCTTATCCAATCCTCTCATGCCCAAGACACACCCCAAGACTACCTCAATTCCCACAACACCGCTCGAGCAGCAGTAGGCGTTGGTCCCTTGACGTGGGATGACAATGTAGCAGGCTATGCACAAAACTACGCCAACCAACATGTTGGCGACTGCAGTCTCGTGCACTCCGGTGGGCCGTACGGCGAAAACCTTGCCATGAGCACTGGTGACTTGTCAGGAACGGCAGCTGTGGACCTGTGGGTGGCGGAGAAAGCCGACTACAATTATGAGTCGAACTCGTGTGCTGATGGAAAGGTGTGTGGGCATTATACACAGGTGGTTTGGCGTAACTCGGCTCGTGTAGGGTGCGCAAAAGTGAGGTGCAGCAGTGGGGGTACCTTCATTGGATGCAACTATGATCCCCCAGGCAACTATGTTGGGGAGAAACCGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

195

Amino Acids

20.85

Weight (kDa)

5.92

Isoelectric Point (pI)

31.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 41
Acc16I TGCGCA 1 cut(s) 499
Acc65I GGTACC 1 cut(s) 524
AccB1I GGYRCC 1 cut(s) 524
AccBSI CCGCTC 1 cut(s) 212
AciI CCGC 4 cut(s) 33, 50, 210, 395
AclWI GGATC 1 cut(s) 542
AfaI GTAC 3 cut(s) 329, 526, 581
AfiI CCNNNNNNNGG 2 cut(s) 243, 391
AflIII ACRYGT 1 cut(s) 289
AjiI CACGTC 1 cut(s) 243
AjnI CCWGG 1 cut(s) 553
AleI CACNNNNGTG 1 cut(s) 318
AluBI AGCT 2 cut(s) 125, 377
AluI AGCT 2 cut(s) 125, 377
Alw21I GWGCWC 2 cut(s) 315, 353
Alw26I GTCTC 1 cut(s) 311
Alw44I GTGCAC 1 cut(s) 311
AlwI GGATC 1 cut(s) 542
Ama87I CYCGRG 1 cut(s) 213
AoxI GGCC 1 cut(s) 323
ApaLI GTGCAC 1 cut(s) 311
ApeKI GCWGC 3 cut(s) 218, 374, 513
Asp718I GGTACC 1 cut(s) 524
AspLEI GCGC 1 cut(s) 500
AspS9I GGNCC 3 cut(s) 233, 323, 382
AsuHPI GGTGA 1 cut(s) 368
AvaI CYCGRG 1 cut(s) 213
AvaII GGWCC 2 cut(s) 233, 382
BaeGI GKGCMC 1 cut(s) 315
BanI GGYRCC 1 cut(s) 524
BauI CACGAG 3 cut(s) 308, 427, 486
Bbv12I GWGCWC 2 cut(s) 315, 353
BbvI GCAGC 3 cut(s) 230, 386, 525
BccI CCATC 2 cut(s) 81, 431
BceAI ACGGC 3 cut(s) 310, 346, 387
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BciT130I CCWGG 1 cut(s) 555
BcoDI GTCTC 1 cut(s) 311
BfaI CTAG 3 cut(s) 9, 122, 583
BfmI CTRYAG 1 cut(s) 301
BisI GCNGC 3 cut(s) 219, 375, 514
BlsI GCNGC 3 cut(s) 220, 376, 515
Bme1390I CCNGG 1 cut(s) 555
Bme18I GGWCC 2 cut(s) 233, 382
BmeT110I CYCGRG 1 cut(s) 213
BmgBI CACGTC 1 cut(s) 243
BmgT120I GGNCC 3 cut(s) 233, 323, 382
BmiI GGNNCC 2 cut(s) 235, 526
BmrFI CCNGG 1 cut(s) 555
BmsI GCATC 1 cut(s) 527
BoxI GACNNNNGTC 1 cut(s) 303
BplI GAGNNNNNCTC 2 cut(s) 410, 442
BsaJI CCNNGG 1 cut(s) 553
BsaWI WCCGGW 1 cut(s) 317
BsaXI ACNNNNNCTCC 1 cut(s) 563
Bsc4I CCNNNNNNNGG 2 cut(s) 243, 391
Bse1I ACTGG 1 cut(s) 358
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 1 cut(s) 553
BseGI GGATG 3 cut(s) 73, 253, 542
BseLI CCNNNNNNNGG 2 cut(s) 243, 391
BseMII CTCAG 1 cut(s) 159
BseNI ACTGG 1 cut(s) 358
BseSI GKGCMC 1 cut(s) 315
BseXI GCAGC 3 cut(s) 230, 386, 525
BsgI GTGCAG 1 cut(s) 532
BshFI GGCC 1 cut(s) 325
BshNI GGYRCC 1 cut(s) 524
BsiHKAI GWGCWC 2 cut(s) 315, 353
BsiHKCI CYCGRG 1 cut(s) 213
BsiSI CCGG 1 cut(s) 318
BsiWI CGTACG 1 cut(s) 327
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 2 cut(s) 243, 391
BsmAI GTCTC 1 cut(s) 311
BsmFI GGGAC 1 cut(s) 219
BsnI GGCC 1 cut(s) 325
BsoBI CYCGRG 1 cut(s) 213
Bsp1286I GDGCHC 2 cut(s) 315, 353
Bsp143I GATC 1 cut(s) 547
BspACI CCGC 4 cut(s) 33, 50, 210, 395
BspANI GGCC 1 cut(s) 325
BspCNI CTCAG 1 cut(s) 158
BspLI GGNNCC 2 cut(s) 235, 526
BspMAI CTGCAG 1 cut(s) 305
BspPI GGATC 1 cut(s) 542
BspT107I GGYRCC 1 cut(s) 524
BsrBI CCGCTC 1 cut(s) 212
BsrI ACTGG 1 cut(s) 358
BssECI CCNNGG 1 cut(s) 553
BssMI GATC 1 cut(s) 547
BssSI CACGAG 3 cut(s) 308, 427, 486
Bst2BI CACGAG 3 cut(s) 308, 427, 486
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 2 cut(s) 27, 579
BstAPI GCANNNNNTGC 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 262
BstDEI CTNAG 1 cut(s) 145
BstF5I GGATG 3 cut(s) 73, 253, 542
BstHHI GCGC 1 cut(s) 500
BstKTI GATC 1 cut(s) 550
BstMAI GTCTC 1 cut(s) 311
BstMBI GATC 1 cut(s) 547
BstMWI GCNNNNNNNGC 3 cut(s) 218, 266, 401
BstNI CCWGG 1 cut(s) 555
BstNSI RCATGY 1 cut(s) 293
BstPAI GACNNNNGTC 1 cut(s) 303
BstSCI CCNGG 1 cut(s) 553
BstSFI CTRYAG 1 cut(s) 301
BstSLI GKGCMC 1 cut(s) 315
BstV1I GCAGC 3 cut(s) 230, 386, 525
BstXI CCANNNNNNTGG 2 cut(s) 100, 293
BsuRI GGCC 1 cut(s) 325
BtrI CACGTC 1 cut(s) 243
BtsCI GGATG 3 cut(s) 73, 253, 542
BtsI GCAGTG 1 cut(s) 523
BtsIMutI CAGTG 2 cut(s) 351, 523
Cac8I GCNNGC 1 cut(s) 262
CfoI GCGC 1 cut(s) 500
Cfr13I GGNCC 3 cut(s) 233, 323, 382
Csp6I GTAC 3 cut(s) 328, 525, 580
CviAII CATG 4 cut(s) 100, 167, 290, 346
CviJI RGCY 7 cut(s) 125, 144, 264, 325, 377, 404, 485
CviKI_1 RGCY 7 cut(s) 125, 144, 264, 325, 377, 404, 485
CviQI GTAC 3 cut(s) 328, 525, 580
DdeI CTNAG 1 cut(s) 145
DpnI GATC 1 cut(s) 549
DpnII GATC 1 cut(s) 547
EciI GGCGGA 1 cut(s) 410
Eco47I GGWCC 2 cut(s) 233, 382
Eco88I CYCGRG 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 553
FaeI CATG 4 cut(s) 103, 170, 293, 349
FaqI GGGAC 1 cut(s) 219
FatI CATG 4 cut(s) 99, 166, 289, 345
FauI CCCGC 1 cut(s) 40
Fnu4HI GCNGC 3 cut(s) 219, 375, 514
FokI GGATG 3 cut(s) 60, 260, 549
Fsp4HI GCNGC 3 cut(s) 219, 375, 514
FspBI CTAG 3 cut(s) 9, 122, 583
FspI TGCGCA 1 cut(s) 499
GlaI GCGC 1 cut(s) 499
GluI GCNGC 3 cut(s) 219, 375, 514
HaeIII GGCC 1 cut(s) 325
HapII CCGG 1 cut(s) 318
HhaI GCGC 1 cut(s) 500
Hin1II CATG 4 cut(s) 103, 170, 293, 349
Hin6I GCGC 1 cut(s) 498
HinP1I GCGC 1 cut(s) 498
HincII GTYRAC 1 cut(s) 17
HindII GTYRAC 1 cut(s) 17
HinfI GANTC 1 cut(s) 419
HpaII CCGG 1 cut(s) 318
HphI GGTGA 1 cut(s) 368
Hpy166II GTNNAC 3 cut(s) 17, 313, 382
Hpy188III TCNNGA 1 cut(s) 366
Hpy8I GTNNAC 3 cut(s) 17, 313, 382
HpyAV CCTTC 1 cut(s) 538
HpyCH4III ACNGT 2 cut(s) 27, 579
HpyCH4IV ACGT 1 cut(s) 242
HpyCH4V TGCA 6 cut(s) 111, 269, 303, 313, 513, 540
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 266, 401
HpyF3I CTNAG 1 cut(s) 145
HpySE526I ACGT 1 cut(s) 242
Hsp92II CATG 4 cut(s) 103, 170, 293, 349
HspAI GCGC 1 cut(s) 498
KpnI GGTACC 1 cut(s) 528
Kzo9I GATC 1 cut(s) 547
LpnPI CCDG 8 cut(s) 246, 331, 339, 351, 398, 449, 540, 567
Lsp1109I GCAGC 3 cut(s) 230, 386, 525
LweI GCATC 1 cut(s) 527
MaeI CTAG 3 cut(s) 9, 122, 583
MaeII ACGT 1 cut(s) 242
MaeIII GTNAC 2 cut(s) 356, 476
MalI GATC 1 cut(s) 549
MbiI CCGCTC 1 cut(s) 212
MboI GATC 1 cut(s) 547
MhlI GDGCHC 2 cut(s) 315, 353
MluCI AATT 2 cut(s) 196, 412
MlyI GAGTC 1 cut(s) 428
MnlI CCTC 3 cut(s) 172, 203, 501
MseI TTAA 1 cut(s) 96
MslI CAYNNNNRTG 2 cut(s) 98, 318
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 1 cut(s) 318
MspR9I CCNGG 1 cut(s) 555
MvaI CCWGG 1 cut(s) 555
MwoI GCNNNNNNNGC 3 cut(s) 218, 266, 401
NdeII GATC 1 cut(s) 547
NlaIII CATG 4 cut(s) 103, 170, 293, 349
NlaIV GGNNCC 2 cut(s) 235, 526
NmeAIII GCCGAG 1 cut(s) 461
NmuCI GTSAC 1 cut(s) 356
NsbI TGCGCA 1 cut(s) 499
NspI RCATGY 1 cut(s) 293
OliI CACNNNNGTG 1 cut(s) 318
PaeR7I CTCGAG 1 cut(s) 213
PciI ACATGT 1 cut(s) 289
Pfl23II CGTACG 1 cut(s) 327
PkrI GCNGC 3 cut(s) 220, 376, 515
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
PscI ACATGT 1 cut(s) 289
PshAI GACNNNNGTC 1 cut(s) 303
PsiI TTATAA 1 cut(s) 41
Psp6I CCWGG 1 cut(s) 553
PspGI CCWGG 1 cut(s) 553
PspLI CGTACG 1 cut(s) 327
PspN4I GGNNCC 2 cut(s) 235, 526
PspPI GGNCC 3 cut(s) 233, 323, 382
PspXI VCTCGAGB 1 cut(s) 213
PstI CTGCAG 1 cut(s) 305
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 3 cut(s) 329, 526, 581
RsaNI GTAC 3 cut(s) 328, 525, 580
RseI CAYNNNNRTG 2 cut(s) 98, 318
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 3 cut(s) 219, 375, 514
Sau3AI GATC 1 cut(s) 547
Sau96I GGNCC 3 cut(s) 233, 323, 382
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 1 cut(s) 555
SduI GDGCHC 2 cut(s) 315, 353
SfaNI GCATC 1 cut(s) 527
SfcI CTRYAG 1 cut(s) 301
Sfr274I CTCGAG 1 cut(s) 213
SinI GGWCC 2 cut(s) 233, 382
SlaI CTCGAG 1 cut(s) 213
SmiMI CAYNNNNRTG 2 cut(s) 98, 318
SmlI CTYRAG 1 cut(s) 213
SmoI CTYRAG 1 cut(s) 213
Sse9I AATT 2 cut(s) 196, 412
SsiI CCGC 4 cut(s) 33, 50, 210, 395
SspI AATATT 1 cut(s) 115
SspMI CTAG 3 cut(s) 9, 122, 583
StyD4I CCNGG 1 cut(s) 553
TaaI ACNGT 2 cut(s) 27, 579
TaiI ACGT 1 cut(s) 245
TaqI TCGA 2 cut(s) 214, 422
TasI AATT 2 cut(s) 196, 412
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 2 cut(s) 358, 523
TseFI GTSAC 1 cut(s) 356
TseI GCWGC 3 cut(s) 218, 374, 513
Tsp45I GTSAC 1 cut(s) 356
TspDTI ATGAA 2 cut(s) 124, 520
TspRI CASTG 2 cut(s) 358, 523
VneI GTGCAC 1 cut(s) 311
VpaK11BI GGWCC 2 cut(s) 233, 382
XceI RCATGY 1 cut(s) 293
XhoI CTCGAG 1 cut(s) 213
XspI CTAG 3 cut(s) 9, 122, 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.