Belongs to the CRISP family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 370 |
| Acc65I | GGTACC | 1 cut(s) | 440 |
| AccB1I | GGYRCC | 1 cut(s) | 440 |
| AccII | CGCG | 2 cut(s) | 123, 158 |
| AciI | CCGC | 2 cut(s) | 121, 270 |
| AclWI | GGATC | 1 cut(s) | 458 |
| AcoI | YGGCCR | 1 cut(s) | 27 |
| AcsI | RAATTY | 1 cut(s) | 522 |
| AfaI | GTAC | 1 cut(s) | 442 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 127, 450 |
| AflIII | ACRYGT | 1 cut(s) | 300 |
| AgsI | TTSAA | 1 cut(s) | 10 |
| AjnI | CCWGG | 3 cut(s) | 126, 469, 536 |
| AluBI | AGCT | 1 cut(s) | 19 |
| AluI | AGCT | 1 cut(s) | 19 |
| Alw21I | GWGCWC | 1 cut(s) | 222 |
| Alw26I | GTCTC | 1 cut(s) | 67 |
| AlwI | GGATC | 1 cut(s) | 458 |
| AoxI | GGCC | 5 cut(s) | 27, 117, 230, 249, 318 |
| ApoI | RAATTY | 1 cut(s) | 522 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 320 |
| Asp718I | GGTACC | 1 cut(s) | 440 |
| AspLEI | GCGC | 2 cut(s) | 125, 212 |
| AspS9I | GGNCC | 2 cut(s) | 118, 230 |
| AsuHPI | GGTGA | 3 cut(s) | 251, 259, 556 |
| AxyI | CCTNAGG | 1 cut(s) | 253 |
| BaeGI | GKGCMC | 1 cut(s) | 287 |
| BalI | TGGCCA | 1 cut(s) | 29 |
| BanI | GGYRCC | 1 cut(s) | 440 |
| BauI | CACGAG | 1 cut(s) | 402 |
| Bbv12I | GWGCWC | 1 cut(s) | 222 |
| BccI | CCATC | 3 cut(s) | 38, 86, 347 |
| BceAI | ACGGC | 1 cut(s) | 278 |
| BciT130I | CCWGG | 3 cut(s) | 128, 471, 538 |
| BcoDI | GTCTC | 1 cut(s) | 67 |
| BfaI | CTAG | 1 cut(s) | 164 |
| BfmI | CTRYAG | 1 cut(s) | 66 |
| BfuAI | ACCTGC | 1 cut(s) | 370 |
| Bme1390I | CCNGG | 3 cut(s) | 128, 471, 538 |
| BmgT120I | GGNCC | 2 cut(s) | 118, 230 |
| BmiI | GGNNCC | 2 cut(s) | 231, 442 |
| BmrFI | CCNGG | 3 cut(s) | 128, 471, 538 |
| BmsI | GCATC | 2 cut(s) | 273, 280 |
| Bsa29I | ATCGAT | 1 cut(s) | 90 |
| BsaJI | CCNNGG | 2 cut(s) | 126, 213 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 127, 450 |
| Bse21I | CCTNAGG | 1 cut(s) | 253 |
| BseBI | CCWGG | 3 cut(s) | 128, 471, 538 |
| BseCI | ATCGAT | 1 cut(s) | 90 |
| BseDI | CCNNGG | 2 cut(s) | 126, 213 |
| BseGI | GGATG | 2 cut(s) | 295, 553 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 127, 450 |
| BseMII | CTCAG | 1 cut(s) | 267 |
| BseSI | GKGCMC | 1 cut(s) | 287 |
| Bsh1236I | CGCG | 2 cut(s) | 123, 158 |
| BshFI | GGCC | 5 cut(s) | 29, 119, 232, 251, 320 |
| BshNI | GGYRCC | 1 cut(s) | 440 |
| BshVI | ATCGAT | 1 cut(s) | 90 |
| BsiHKAI | GWGCWC | 1 cut(s) | 222 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 127, 450 |
| BsmAI | GTCTC | 1 cut(s) | 67 |
| BsnI | GGCC | 5 cut(s) | 29, 119, 232, 251, 320 |
| Bsp1286I | GDGCHC | 2 cut(s) | 222, 287 |
| Bsp143I | GATC | 1 cut(s) | 463 |
| Bsp19I | CCATGG | 1 cut(s) | 213 |
| BspACI | CCGC | 2 cut(s) | 121, 270 |
| BspANI | GGCC | 5 cut(s) | 29, 119, 232, 251, 320 |
| BspCNI | CTCAG | 1 cut(s) | 266 |
| BspDI | ATCGAT | 1 cut(s) | 90 |
| BspFNI | CGCG | 2 cut(s) | 123, 158 |
| BspLI | GGNNCC | 2 cut(s) | 231, 442 |
| BspMAI | CTGCAG | 1 cut(s) | 70 |
| BspMI | ACCTGC | 1 cut(s) | 370 |
| BspPI | GGATC | 1 cut(s) | 458 |
| BspT107I | GGYRCC | 1 cut(s) | 440 |
| BssECI | CCNNGG | 2 cut(s) | 126, 213 |
| BssMI | GATC | 1 cut(s) | 463 |
| BssSI | CACGAG | 1 cut(s) | 402 |
| BssT1I | CCWWGG | 1 cut(s) | 213 |
| Bst2BI | CACGAG | 1 cut(s) | 402 |
| Bst2UI | CCWGG | 3 cut(s) | 128, 471, 538 |
| Bst4CI | ACNGT | 2 cut(s) | 140, 434 |
| BstC8I | GCNNGC | 1 cut(s) | 121 |
| BstDEI | CTNAG | 1 cut(s) | 253 |
| BstDSI | CCRYGG | 1 cut(s) | 213 |
| BstF5I | GGATG | 2 cut(s) | 295, 553 |
| BstFNI | CGCG | 2 cut(s) | 123, 158 |
| BstHHI | GCGC | 2 cut(s) | 125, 212 |
| BstKTI | GATC | 1 cut(s) | 466 |
| BstMAI | GTCTC | 1 cut(s) | 67 |
| BstMBI | GATC | 1 cut(s) | 463 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 164, 173, 317, 376, 499 |
| BstNI | CCWGG | 3 cut(s) | 128, 471, 538 |
| BstNSI | RCATGY | 1 cut(s) | 304 |
| BstSCI | CCNGG | 3 cut(s) | 126, 469, 536 |
| BstSFI | CTRYAG | 1 cut(s) | 66 |
| BstSLI | GKGCMC | 1 cut(s) | 287 |
| BstUI | CGCG | 2 cut(s) | 123, 158 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 150, 544 |
| Bsu15I | ATCGAT | 1 cut(s) | 90 |
| Bsu36I | CCTNAGG | 1 cut(s) | 253 |
| BsuRI | GGCC | 5 cut(s) | 29, 119, 232, 251, 320 |
| BsuTUI | ATCGAT | 1 cut(s) | 90 |
| BtgI | CCRYGG | 1 cut(s) | 213 |
| BtsCI | GGATG | 2 cut(s) | 295, 553 |
| BtsIMutI | CAGTG | 1 cut(s) | 439 |
| BveI | ACCTGC | 1 cut(s) | 370 |
| Cac8I | GCNNGC | 1 cut(s) | 121 |
| CfoI | GCGC | 2 cut(s) | 125, 212 |
| Cfr13I | GGNCC | 2 cut(s) | 118, 230 |
| ClaI | ATCGAT | 1 cut(s) | 90 |
| Csp6I | GTAC | 1 cut(s) | 441 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 346, 381 |
| CviAII | CATG | 4 cut(s) | 214, 301, 531, 544 |
| CviQI | GTAC | 1 cut(s) | 441 |
| DdeI | CTNAG | 1 cut(s) | 253 |
| DpnI | GATC | 1 cut(s) | 465 |
| DpnII | GATC | 1 cut(s) | 463 |
| EaeI | YGGCCR | 1 cut(s) | 27 |
| Eco130I | CCWWGG | 1 cut(s) | 213 |
| Eco147I | AGGCCT | 1 cut(s) | 320 |
| Eco32I | GATATC | 1 cut(s) | 88 |
| Eco81I | CCTNAGG | 1 cut(s) | 253 |
| EcoO109I | RGGNCCY | 1 cut(s) | 230 |
| EcoRII | CCWGG | 3 cut(s) | 126, 469, 536 |
| EcoRV | GATATC | 1 cut(s) | 88 |
| EcoT14I | CCWWGG | 1 cut(s) | 213 |
| ErhI | CCWWGG | 1 cut(s) | 213 |
| FaeI | CATG | 4 cut(s) | 217, 304, 534, 547 |
| FatI | CATG | 4 cut(s) | 213, 300, 530, 543 |
| FauI | CCCGC | 1 cut(s) | 128 |
| FokI | GGATG | 2 cut(s) | 302, 560 |
| FspBI | CTAG | 1 cut(s) | 164 |
| GlaI | GCGC | 2 cut(s) | 124, 211 |
| HaeIII | GGCC | 5 cut(s) | 29, 119, 232, 251, 320 |
| HhaI | GCGC | 2 cut(s) | 125, 212 |
| Hin1II | CATG | 4 cut(s) | 217, 304, 534, 547 |
| Hin6I | GCGC | 2 cut(s) | 123, 210 |
| HinP1I | GCGC | 2 cut(s) | 123, 210 |
| HindIII | AAGCTT | 1 cut(s) | 17 |
| HinfI | GANTC | 1 cut(s) | 335 |
| HphI | GGTGA | 3 cut(s) | 251, 259, 556 |
| Hpy166II | GTNNAC | 2 cut(s) | 143, 298 |
| Hpy188III | TCNNGA | 1 cut(s) | 255 |
| Hpy8I | GTNNAC | 2 cut(s) | 143, 298 |
| Hpy99I | CGWCG | 1 cut(s) | 44 |
| HpyAV | CCTTC | 4 cut(s) | 254, 310, 331, 454 |
| HpyCH4III | ACNGT | 2 cut(s) | 140, 434 |
| HpyCH4V | TGCA | 6 cut(s) | 68, 188, 416, 429, 456, 543 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 164, 173, 317, 376, 499 |
| HpyF3I | CTNAG | 1 cut(s) | 253 |
| Hsp92II | CATG | 4 cut(s) | 217, 304, 534, 547 |
| HspAI | GCGC | 2 cut(s) | 123, 210 |
| KpnI | GGTACC | 1 cut(s) | 444 |
| Kzo9I | GATC | 1 cut(s) | 463 |
| LmnI | GCTCC | 2 cut(s) | 41, 217 |
| LpnPI | CCDG | 9 cut(s) | 11, 113, 140, 240, 365, 456, 483, 523, 550 |
| LweI | GCATC | 2 cut(s) | 273, 280 |
| MaeI | CTAG | 1 cut(s) | 164 |
| MaeIII | GTNAC | 1 cut(s) | 392 |
| MalI | GATC | 1 cut(s) | 465 |
| MboI | GATC | 1 cut(s) | 463 |
| MboII | GAAGA | 3 cut(s) | 14, 43, 95 |
| MhlI | GDGCHC | 2 cut(s) | 222, 287 |
| MlsI | TGGCCA | 1 cut(s) | 29 |
| MluCI | AATT | 1 cut(s) | 522 |
| MluNI | TGGCCA | 1 cut(s) | 29 |
| MlyI | GAGTC | 1 cut(s) | 329 |
| MnlI | CCTC | 4 cut(s) | 97, 262, 417, 431 |
| Mox20I | TGGCCA | 1 cut(s) | 29 |
| MroXI | GAANNNNTTC | 1 cut(s) | 320 |
| MscI | TGGCCA | 1 cut(s) | 29 |
| MslI | CAYNNNNRTG | 2 cut(s) | 148, 305 |
| Msp20I | TGGCCA | 1 cut(s) | 29 |
| MspA1I | CMGCKG | 1 cut(s) | 272 |
| MspR9I | CCNGG | 3 cut(s) | 128, 471, 538 |
| MvaI | CCWGG | 3 cut(s) | 128, 471, 538 |
| MvnI | CGCG | 2 cut(s) | 123, 158 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 164, 173, 317, 376, 499 |
| NcoI | CCATGG | 1 cut(s) | 213 |
| NdeII | GATC | 1 cut(s) | 463 |
| NlaIII | CATG | 4 cut(s) | 217, 304, 534, 547 |
| NlaIV | GGNNCC | 2 cut(s) | 231, 442 |
| NspI | RCATGY | 1 cut(s) | 304 |
| PceI | AGGCCT | 1 cut(s) | 320 |
| PciI | ACATGT | 1 cut(s) | 300 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 96 |
| PdmI | GAANNNNTTC | 1 cut(s) | 320 |
| PleI | GAGTC | 1 cut(s) | 329 |
| PpsI | GAGTC | 1 cut(s) | 329 |
| PscI | ACATGT | 1 cut(s) | 300 |
| Psp6I | CCWGG | 3 cut(s) | 126, 469, 536 |
| PspGI | CCWGG | 3 cut(s) | 126, 469, 536 |
| PspN4I | GGNNCC | 2 cut(s) | 231, 442 |
| PspPI | GGNCC | 2 cut(s) | 118, 230 |
| PstI | CTGCAG | 1 cut(s) | 70 |
| RsaI | GTAC | 1 cut(s) | 442 |
| RsaNI | GTAC | 1 cut(s) | 441 |
| RseI | CAYNNNNRTG | 2 cut(s) | 148, 305 |
| Sau3AI | GATC | 1 cut(s) | 463 |
| Sau96I | GGNCC | 2 cut(s) | 118, 230 |
| SchI | GAGTC | 1 cut(s) | 329 |
| ScrFI | CCNGG | 3 cut(s) | 128, 471, 538 |
| SduI | GDGCHC | 2 cut(s) | 222, 287 |
| SetI | ASST | 9 cut(s) | 21, 75, 132, 363, 384, 428, 442, 446, 542 |
| SfaNI | GCATC | 2 cut(s) | 273, 280 |
| SfcI | CTRYAG | 1 cut(s) | 66 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 148, 305 |
| Sse9I | AATT | 1 cut(s) | 522 |
| SseBI | AGGCCT | 1 cut(s) | 320 |
| SsiI | CCGC | 2 cut(s) | 121, 270 |
| SspI | AATATT | 1 cut(s) | 561 |
| SspMI | CTAG | 1 cut(s) | 164 |
| StuI | AGGCCT | 1 cut(s) | 320 |
| StyD4I | CCNGG | 3 cut(s) | 126, 469, 536 |
| StyI | CCWWGG | 1 cut(s) | 213 |
| TaaI | ACNGT | 2 cut(s) | 140, 434 |
| TaqI | TCGA | 1 cut(s) | 90 |
| TasI | AATT | 1 cut(s) | 522 |
| TscAI | CASTG | 1 cut(s) | 439 |
| TspDTI | ATGAA | 1 cut(s) | 436 |
| TspGWI | ACGGA | 2 cut(s) | 28, 302 |
| TspRI | CASTG | 1 cut(s) | 439 |
| XapI | RAATTY | 1 cut(s) | 522 |
| XceI | RCATGY | 1 cut(s) | 304 |
| XmnI | GAANNNNTTC | 1 cut(s) | 320 |
| XspI | CTAG | 1 cut(s) | 164 |