Rorug05G0527800

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001302
Physical Location & Seq
Reverse (-)
71643144 .. 71645593
2450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0527800.1

Sequence Viewer

Length: 1884 bp
ATGTATGTCAGAGTTTCAGAAAAGAAAATTTCAAAATTTAATTGTGAACGGAATATAGGAAGGAAAAGGCACAGAAATCGGATCAGAAACTCAAAGCAACTGTTGAATGTGGAGATGATGCGCAGCCGCAGCACAAGAGGTATGCTTTCTTCTCTTCCCTCTACTCATCTTGCCGTTCCACTCATCAACAATTTCATATCTTTGTTTCATTCGAGACAAACCCATCTAGGAAATCGACCCAATGTTATTATTGTGAGTAATGTTGAGGATGCCTCCACGTTGTTCCATCAAATGCTTCACTCCCGTCCTCCTCTGCCTTCTTCTGTTGTCCCTTTCACTCAGATATTGGGTCAACTTGTCAAATTGAAACACTATTCGGCTGTCATCCCTCTCTTTAAACAAATGCTTCTGCGTCGAATTGTTCCTAATCTCTATACTCTCTCTATTGTCATCAACTGCTACTGCCATTTGAATCAAATGGGCTTTGGCTTATCTGTCTTTGCACAATTCTTCAAACTGGGTCTTCAGCCAGACGTCTTCACCTTCACCACTCTAATCAACGGCTTTGTTCTCCACAATCAAGTGCCTCAGGCTGCACGAATTTTCACCAAAATGGTCGAGGCAGGTCATTGTCAGCCGGATGTGTTCGCTTTCAACACACTAATAAAGGGCTTTTCCAAGATGGGAAACAACAGTGCGGCCATGCAATTACTTAGGAAGATGGAAGAAAGAGGATGCGAGCCTAACATGGTTTCCTATAGCACCATCATCGACGGTCTTTGCAAGGATACACTAATTGATCAAGCATTCAACCTCTTCTCAGAAATGATTACTAGAGGTATTGCTCCAAACGTCCTTACTTACAACTCCTTGATTCAAGGAGTTTGCAATATAGGCCACTGGAAACAAGCTACGAGGTTGTTGAACGAAATGGTGAGTAAAGGTATCTTTCCAGATGTCCGCACCTTCAATATCTTGGTTGATACATTCTGTAAGGAAGGGATGGTCGTGGAAGCCAAAACTGTGGTTCAAATCATGATTCAAAGACATATTGAACCTACTGTCGTTACATACAACTCACTTATGGACGGTTACTGTTTGCGAGGAGAAATGGACGAGGCAAGACAAGTTTTTGATCTTATCATTAGCAAGGGCTCCACGGTTAATGTTCGGAGTTGTAACATATTGATTAACGGATATTGCAAGGCTAAAAAGGTTGATAAGGCTCACAAGATTTTCAAGGAAATGCGTCGTATGGAAATTGTTCCCGATACCATTACTTATAACACTCTTATTGATGGTCTTTTCAAAATGGGGAGAATTCAAGAAGCAGAAAAGCTGTTCTCTGAGATGCTAGGTTGTGGACAGCTTCCAAACCTTCGAACTTACACTGTCATACTTGATGGCCTGTGTAACAACCAGCAACTTTCTATGGCTATAGAATTGCTTAAAGAAATGGAAGCCAACAAAGTAGAACTTAATATCGTAGTTTACACTCTTGTTATTGAAGGTTTGTGCAAAGCTGGAAAAATTGAATCCGCAAGAGATCTCTTCTGTGGTTTGTCGTCAAAAGGAGTTCAGCCTAATGTCAGAACATACACTGTAATGATTCATGGACTTTGTCATCATGGCTTAATAATTGAAGCAGAAAAGTTGCTAAGAGAAATGGGAGGGAAAGGATGTTCTCCAAATGATTGCACCTATAACACCATTATCCGAGGGTTGTTAAATAACAATGAGACGTCATGGGCTACGAGACTTATACAAGAAATGCTTGAGAGGGGTTTCTCTGCAGATGCATCAACTATGGAATTGATTGTTGATTTATTGTCAAAGGATATAGTAGATCCGGCTTTGTTGCCACTGTTAAAAGATTCACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

627

Amino Acids

70.82

Weight (kDa)

9.12

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 108 - 156 8.5e-07 PPR repeat family
PPR_2 PF13041 149 - 189 5.3e-07 PPR repeat family
PPR_3 PF13812 171 - 226 1e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 174 - 206 1.4e-06 PPR repeat
PPR_3 PF13812 211 - 258 9.7e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 211 - 242 1.2e-08 PPR repeat
PPR_2 PF13041 213 - 262 1.1e-17 PPR repeat family
PPR PF01535 217 - 246 3e-06 PPR repeat
PPR_1 PF12854 244 - 276 4e-13 PPR repeat
PPR PF01535 251 - 281 3.4e-06 PPR repeat
PPR_2 PF13041 251 - 297 7.1e-16 PPR repeat family
PPR_long PF17177 265 - 362 9.9e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 272 - 331 1.8e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 279 - 312 2.9e-10 PPR repeat
PPR_2 PF13041 283 - 332 6.9e-18 PPR repeat family
MRP-S27 PF10037 287 - 349 3.2e-08 Mitochondrial 28S ribosomal protein S27
PPR PF01535 287 - 316 9.9e-06 PPR repeat
PPR_3 PF13812 306 - 364 4.9e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 314 - 346 9e-10 PPR repeat
PPR_2 PF13041 318 - 366 1.5e-12 PPR repeat family
TPR_24 PF23276 323 - 416 2.5e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 350 - 379 7e-11 PPR repeat
PPR_2 PF13041 353 - 396 6.6e-09 PPR repeat family
PPR PF01535 356 - 385 8.6e-08 PPR repeat
PPR_long PF17177 372 - 508 5.7e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 383 - 433 2.9e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 385 - 417 9.8e-09 PPR repeat
PPR_2 PF13041 389 - 437 6.8e-18 PPR repeat family
PPR PF01535 392 - 419 1.1e-08 PPR repeat
PPR_1 PF12854 420 - 451 9.5e-13 PPR repeat
PPR_2 PF13041 423 - 472 7.4e-18 PPR repeat family
PPR PF01535 426 - 455 1e-08 PPR repeat
PPR_1 PF12854 455 - 487 1.2e-06 PPR repeat
PPR_2 PF13041 466 - 507 1.1e-08 PPR repeat family
PPR_3 PF13812 482 - 537 2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 491 - 518 8e-06 PPR repeat
PPR_2 PF13041 494 - 542 3.8e-16 PPR repeat family
PPR_3 PF13812 521 - 573 4.8e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 524 - 556 4.6e-11 PPR repeat
PPR PF01535 532 - 561 2.4e-08 PPR repeat
PPR_2 PF13041 536 - 576 4.9e-11 PPR repeat family
PPR_1 PF12854 559 - 591 2.3e-06 PPR repeat
PPR_2 PF13041 565 - 610 6.8e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1284
AatII GACGTC 2 cut(s) 537, 1745
Acc16I TGCGCA 1 cut(s) 122
Acc36I ACCTGC 1 cut(s) 614
AciI CCGC 4 cut(s) 127, 698, 961, 1539
AclWI GGATC 2 cut(s) 89, 1841
AcoI YGGCCR 1 cut(s) 699
AcsI RAATTY 4 cut(s) 27, 35, 600, 1320
AcuI CTGAAG 1 cut(s) 509
AcyI GRCGYC 2 cut(s) 534, 1742
AloI GAACNNNNNNTCC 2 cut(s) 1666, 1698
AluBI AGCT 4 cut(s) 911, 1339, 1369, 1523
AluI AGCT 4 cut(s) 911, 1339, 1369, 1523
Alw26I GTCTC 3 cut(s) 208, 1733, 1750
AlwI GGATC 2 cut(s) 89, 1841
AoxI GGCC 3 cut(s) 699, 895, 1405
ApeKI GCWGC 3 cut(s) 123, 129, 593
ApoI RAATTY 4 cut(s) 27, 35, 600, 1320
Asp700I GAANNNNTTC 1 cut(s) 1263
AspLEI GCGC 1 cut(s) 123
AsuHPI GGTGA 4 cut(s) 532, 538, 598, 946
AsuII TTCGAA 1 cut(s) 1381
AxyI CCTNAGG 1 cut(s) 588
BanII GRGCYC 1 cut(s) 1157
BarI GAAGNNNNNNTAC 2 cut(s) 133, 165
BbsI GAAGAC 2 cut(s) 515, 529
BbvI GCAGC 3 cut(s) 135, 141, 580
BccI CCATC 8 cut(s) 231, 294, 676, 715, 773, 997, 1292, 1397
BceAI ACGGC 2 cut(s) 158, 577
BcgI CGANNNNNNTGC 4 cut(s) 59, 93, 751, 785
BciVI GTATCC 1 cut(s) 781
BclI TGATCA 1 cut(s) 799
BcoDI GTCTC 3 cut(s) 208, 1733, 1750
BfaI CTAG 3 cut(s) 227, 834, 1355
BfmI CTRYAG 3 cut(s) 757, 1437, 1791
BfuAI ACCTGC 1 cut(s) 614
BfuI GTATCC 1 cut(s) 781
BglII AGATCT 1 cut(s) 1546
BisI GCNGC 5 cut(s) 124, 127, 130, 594, 699
BlsI GCNGC 5 cut(s) 125, 128, 131, 595, 700
BmiI GGNNCC 1 cut(s) 1156
BmrI ACTGGG 1 cut(s) 527
BmsI GCATC 6 cut(s) 108, 259, 725, 1341, 1786, 1808
BmuI ACTGGG 1 cut(s) 527
BpiI GAAGAC 2 cut(s) 515, 529
BplI GAGNNNNNCTC 2 cut(s) 257, 289
Bpu14I TTCGAA 1 cut(s) 1381
BpuEI CTTGAG 1 cut(s) 1796
BsaHI GRCGYC 2 cut(s) 534, 1742
BsaJI CCNNGG 2 cut(s) 1158, 1717
Bse1I ACTGG 2 cut(s) 522, 905
Bse21I CCTNAGG 1 cut(s) 588
BseDI CCNNGG 2 cut(s) 1158, 1717
BseGI GGATG 6 cut(s) 274, 384, 646, 740, 1008, 1685
BseMII CTCAG 4 cut(s) 353, 602, 834, 1338
BseNI ACTGG 2 cut(s) 522, 905
BseRI GAGGAG 2 cut(s) 300, 1119
BseXI GCAGC 3 cut(s) 135, 141, 580
BsgI GTGCAG 1 cut(s) 579
BshFI GGCC 3 cut(s) 701, 897, 1407
BsiSI CCGG 2 cut(s) 638, 1850
BslFI GGGAC 1 cut(s) 314
BsmAI GTCTC 3 cut(s) 208, 1733, 1750
BsmBI CGTCTC 1 cut(s) 1733
BsmFI GGGAC 1 cut(s) 314
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 3 cut(s) 701, 897, 1407
Bsp119I TTCGAA 1 cut(s) 1381
Bsp1286I GDGCHC 1 cut(s) 1157
Bsp143I GATC 5 cut(s) 81, 799, 1135, 1546, 1846
BspACI CCGC 4 cut(s) 127, 698, 961, 1539
BspANI GGCC 3 cut(s) 701, 897, 1407
BspCNI CTCAG 4 cut(s) 352, 601, 833, 1339
BspHI TCATGA 1 cut(s) 1035
BspLI GGNNCC 1 cut(s) 1156
BspMAI CTGCAG 1 cut(s) 1795
BspMI ACCTGC 1 cut(s) 614
BspPI GGATC 2 cut(s) 89, 1841
BspT104I TTCGAA 1 cut(s) 1381
BsrI ACTGG 2 cut(s) 522, 905
BssECI CCNNGG 2 cut(s) 1158, 1717
BssMI GATC 5 cut(s) 81, 799, 1135, 1546, 1846
BssNI GRCGYC 2 cut(s) 534, 1742
Bst6I CTCTTC 3 cut(s) 159, 821, 1556
BstACI GRCGYC 2 cut(s) 534, 1742
BstBI TTCGAA 1 cut(s) 1381
BstC8I GCNNGC 1 cut(s) 740
BstDEI CTNAG 6 cut(s) 339, 588, 713, 820, 1347, 1658
BstDSI CCRYGG 1 cut(s) 1158
BstF5I GGATG 6 cut(s) 274, 384, 646, 740, 1008, 1685
BstHHI GCGC 1 cut(s) 123
BstKTI GATC 5 cut(s) 84, 802, 1138, 1549, 1849
BstMAI GTCTC 3 cut(s) 208, 1733, 1750
BstMBI GATC 5 cut(s) 81, 799, 1135, 1546, 1846
BstMWI GCNNNNNNNGC 2 cut(s) 129, 894
BstSFI CTRYAG 3 cut(s) 757, 1437, 1791
BstV1I GCAGC 3 cut(s) 135, 141, 580
BstV2I GAAGAC 2 cut(s) 515, 529
BstX2I RGATCY 2 cut(s) 1546, 1846
BstXI CCANNNNNNTGG 1 cut(s) 1024
BstYI RGATCY 2 cut(s) 1546, 1846
Bsu36I CCTNAGG 1 cut(s) 588
BsuI GTATCC 1 cut(s) 781
BsuRI GGCC 3 cut(s) 701, 897, 1407
BtgI CCRYGG 1 cut(s) 1158
BtsCI GGATG 6 cut(s) 274, 384, 646, 740, 1008, 1685
BtsIMutI CAGTG 5 cut(s) 700, 898, 1389, 1599, 1862
BveI ACCTGC 1 cut(s) 614
Cac8I GCNNGC 1 cut(s) 740
CciI TCATGA 1 cut(s) 1035
CfoI GCGC 1 cut(s) 123
CseI GACGC 2 cut(s) 401, 1238
CviAII CATG 6 cut(s) 703, 748, 1036, 1613, 1628, 1746
DdeI CTNAG 6 cut(s) 339, 588, 713, 820, 1347, 1658
DpnI GATC 5 cut(s) 83, 801, 1137, 1548, 1848
DpnII GATC 5 cut(s) 81, 799, 1135, 1546, 1846
DraI TTTAAA 1 cut(s) 397
EaeI YGGCCR 1 cut(s) 699
Eam1104I CTCTTC 3 cut(s) 159, 821, 1556
EarI CTCTTC 3 cut(s) 159, 821, 1556
Eco24I GRGCYC 1 cut(s) 1157
Eco57I CTGAAG 1 cut(s) 509
Eco81I CCTNAGG 1 cut(s) 588
EcoRI GAATTC 1 cut(s) 1320
EcoT22I ATGCAT 1 cut(s) 1801
EcoT38I GRGCYC 1 cut(s) 1157
Esp3I CGTCTC 1 cut(s) 1733
FaeI CATG 6 cut(s) 706, 751, 1039, 1616, 1631, 1749
FalI AAGNNNNNCTT 4 cut(s) 1461, 1493, 1758, 1790
FaqI GGGAC 1 cut(s) 314
FatI CATG 6 cut(s) 702, 747, 1035, 1612, 1627, 1745
FbaI TGATCA 1 cut(s) 799
Fnu4HI GCNGC 5 cut(s) 124, 127, 130, 594, 699
FokI GGATG 6 cut(s) 281, 371, 653, 747, 1015, 1692
FriOI GRGCYC 1 cut(s) 1157
Fsp4HI GCNGC 5 cut(s) 124, 127, 130, 594, 699
FspBI CTAG 3 cut(s) 227, 834, 1355
FspI TGCGCA 1 cut(s) 122
GlaI GCGC 1 cut(s) 122
GluI GCNGC 5 cut(s) 124, 127, 130, 594, 699
HaeIII GGCC 3 cut(s) 701, 897, 1407
HapII CCGG 2 cut(s) 638, 1850
HgaI GACGC 2 cut(s) 401, 1238
HhaI GCGC 1 cut(s) 123
Hin1I GRCGYC 2 cut(s) 534, 1742
Hin1II CATG 6 cut(s) 706, 751, 1039, 1616, 1631, 1749
Hin6I GCGC 1 cut(s) 121
HinP1I GCGC 1 cut(s) 121
HincII GTYRAC 1 cut(s) 353
HindII GTYRAC 1 cut(s) 353
HinfI GANTC 6 cut(s) 472, 874, 1039, 1535, 1609, 1874
HpaII CCGG 2 cut(s) 638, 1850
HphI GGTGA 4 cut(s) 532, 538, 598, 946
Hpy166II GTNNAC 4 cut(s) 47, 353, 1364, 1492
Hpy188III TCNNGA 6 cut(s) 213, 953, 1036, 1268, 1325, 1878
Hpy8I GTNNAC 4 cut(s) 47, 353, 1364, 1492
Hpy99I CGWCG 3 cut(s) 417, 776, 1254
HpyAV CCTTC 7 cut(s) 54, 327, 553, 976, 992, 1388, 1502
HpyCH4IV ACGT 4 cut(s) 278, 534, 852, 1742
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 894
HpyF3I CTNAG 6 cut(s) 339, 588, 713, 820, 1347, 1658
HpySE526I ACGT 4 cut(s) 278, 534, 852, 1742
Hsp92I GRCGYC 2 cut(s) 534, 1742
Hsp92II CATG 6 cut(s) 706, 751, 1039, 1616, 1631, 1749
HspAI GCGC 1 cut(s) 121
Ksp22I TGATCA 1 cut(s) 799
Kzo9I GATC 5 cut(s) 81, 799, 1135, 1546, 1846
LmnI GCTCC 2 cut(s) 850, 1160
Lsp1109I GCAGC 3 cut(s) 135, 141, 580
LweI GCATC 6 cut(s) 108, 259, 725, 1341, 1786, 1808
MaeI CTAG 3 cut(s) 227, 834, 1355
MaeII ACGT 4 cut(s) 278, 534, 852, 1742
MaeIII GTNAC 4 cut(s) 1066, 1091, 1178, 1412
MalI GATC 5 cut(s) 83, 801, 1137, 1548, 1848
MboI GATC 5 cut(s) 81, 799, 1135, 1546, 1846
MflI RGATCY 2 cut(s) 1546, 1846
MhlI GDGCHC 1 cut(s) 1157
Mph1103I ATGCAT 1 cut(s) 1801
MroXI GAANNNNTTC 1 cut(s) 1263
MseI TTAA 9 cut(s) 39, 396, 1164, 1191, 1449, 1479, 1634, 1727, 1868
MslI CAYNNNNRTG 2 cut(s) 611, 1604
MspI CCGG 2 cut(s) 638, 1850
Mva1269I GAATGC 1 cut(s) 806
MwoI GCNNNNNNNGC 2 cut(s) 129, 894
NdeII GATC 5 cut(s) 81, 799, 1135, 1546, 1846
NlaIII CATG 6 cut(s) 706, 751, 1039, 1616, 1631, 1749
NlaIV GGNNCC 1 cut(s) 1156
NsbI TGCGCA 1 cut(s) 122
NsiI ATGCAT 1 cut(s) 1801
NspV TTCGAA 1 cut(s) 1381
PagI TCATGA 1 cut(s) 1035
PctI GAATGC 1 cut(s) 806
PdmI GAANNNNTTC 1 cut(s) 1263
PfeI GAWTC 6 cut(s) 472, 874, 1039, 1535, 1609, 1874
PflFI GACNNNGTC 1 cut(s) 1620
PkrI GCNGC 5 cut(s) 125, 128, 131, 595, 700
PsiI TTATAA 1 cut(s) 1284
PspN4I GGNNCC 1 cut(s) 1156
PstI CTGCAG 1 cut(s) 1795
PsuI RGATCY 2 cut(s) 1546, 1846
PsyI GACNNNGTC 1 cut(s) 1620
RseI CAYNNNNRTG 2 cut(s) 611, 1604
SaqAI TTAA 9 cut(s) 39, 396, 1164, 1191, 1449, 1479, 1634, 1727, 1868
SatI GCNGC 5 cut(s) 124, 127, 130, 594, 699
Sau3AI GATC 5 cut(s) 81, 799, 1135, 1546, 1846
SduI GDGCHC 1 cut(s) 1157
SfaNI GCATC 6 cut(s) 108, 259, 725, 1341, 1786, 1808
SfcI CTRYAG 3 cut(s) 757, 1437, 1791
SfuI TTCGAA 1 cut(s) 1381
SmiMI CAYNNNNRTG 2 cut(s) 611, 1604
SmlI CTYRAG 1 cut(s) 1775
SmoI CTYRAG 1 cut(s) 1775
SsiI CCGC 4 cut(s) 127, 698, 961, 1539
SspMI CTAG 3 cut(s) 227, 834, 1355
TaiI ACGT 4 cut(s) 281, 537, 855, 1745
TaqI TCGA 6 cut(s) 212, 235, 415, 618, 771, 1381
TauI GCSGC 2 cut(s) 129, 701
TfiI GAWTC 6 cut(s) 472, 874, 1039, 1535, 1609, 1874
Tru1I TTAA 9 cut(s) 39, 396, 1164, 1191, 1449, 1479, 1634, 1727, 1868
Tru9I TTAA 9 cut(s) 39, 396, 1164, 1191, 1449, 1479, 1634, 1727, 1868
TscAI CASTG 5 cut(s) 700, 905, 1396, 1606, 1869
TseI GCWGC 3 cut(s) 123, 129, 593
TspDTI ATGAA 3 cut(s) 184, 197, 1601
TspGWI ACGGA 2 cut(s) 64, 1209
TspRI CASTG 5 cut(s) 700, 905, 1396, 1606, 1869
Tth111I GACNNNGTC 1 cut(s) 1620
XapI RAATTY 4 cut(s) 27, 35, 600, 1320
XmnI GAANNNNTTC 1 cut(s) 1263
XspI CTAG 3 cut(s) 227, 834, 1355
ZraI GACGTC 2 cut(s) 535, 1743
Zsp2I ATGCAT 1 cut(s) 1801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.