AT4G33730

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001302
Physical Location & Seq
Forward (+)
16185044 .. 16185654
611 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33730.1

Sequence Viewer

Length: 519 bp
ATGTTTCATAAGGTTGCTATTGAAACCTTGGTTCTCCTACTTCTCATCAATTACTTGACACAAATCGATGTTTCCTCCGCACAATACTCTCAGTATCCTCAGTCCCATGAATATCCTGACAGCTACCTTAGACCCCACAACGCAGCCCGAGCCGCGGTCAAAGTCAAGCCTCTAAGATGGGATTTCGGTATAGCCACTGTGGCTCAGGACTACGCAAACCATCTAGCCTCAGGTCCATGCAGTCTTGAACATTCCTCTGGACCATATGGAGAGAATCTTGCATTTGGCAGTGGAGACATGTCGGCCGCTCAGGCGGTTGCGATGTGGGTACATGAGAAGTCGTACTATGATTTTTACTCAAACTCGTGCCATGGTCCGGCTTGTGGACACTACACGCAGGTCGTGTGGCGTGGCTCAGCTCGTCTCGGTTGTGGTAAGGCTAAGTGTAATAATGGTGCCAGTATTGTTGTGTGTAACTATGACCCCGCAGGCAATTACATTGGAGCTAGACCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

172

Amino Acids

18.83

Weight (kDa)

7.06

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 43 - 160 9.3e-21 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 388
AccB1I GGYRCC 1 cut(s) 455
AccBSI CCGCTC 1 cut(s) 308
AccII CGCG 1 cut(s) 155
AciI CCGC 6 cut(s) 78, 153, 155, 306, 314, 486
AcoI YGGCCR 1 cut(s) 303
AfaI GTAC 2 cut(s) 330, 344
AfiI CCNNNNNNNGG 3 cut(s) 154, 376, 383
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 2 cut(s) 23, 248
AluBI AGCT 3 cut(s) 123, 419, 506
AluI AGCT 3 cut(s) 123, 419, 506
Alw26I GTCTC 2 cut(s) 288, 428
Ama87I CYCGRG 1 cut(s) 147
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 1 cut(s) 143
AspS9I GGNCC 3 cut(s) 233, 260, 374
AvaI CYCGRG 1 cut(s) 147
AvaII GGWCC 3 cut(s) 233, 260, 374
AxyI CCTNAGG 1 cut(s) 229
BanI GGYRCC 1 cut(s) 455
BauI CACGAG 1 cut(s) 364
BbvI GCAGC 1 cut(s) 155
BccI CCATC 2 cut(s) 171, 228
BciVI GTATCC 1 cut(s) 105
BcoDI GTCTC 2 cut(s) 288, 428
BfaI CTAG 2 cut(s) 224, 507
BfuAI ACCTGC 1 cut(s) 388
BfuI GTATCC 1 cut(s) 105
BglI GCCNNNNNGGC 2 cut(s) 200, 311
BisI GCNGC 3 cut(s) 144, 153, 306
BlpI GCTNAGC 1 cut(s) 415
BlsI GCNGC 3 cut(s) 145, 154, 307
Bme18I GGWCC 3 cut(s) 233, 260, 374
BmeT110I CYCGRG 1 cut(s) 147
BmgT120I GGNCC 3 cut(s) 233, 260, 374
BmiI GGNNCC 1 cut(s) 457
Bpu10I CCTNAGC 2 cut(s) 204, 309
Bpu1102I GCTNAGC 1 cut(s) 415
Bsa29I ATCGAT 1 cut(s) 66
BsaJI CCNNGG 3 cut(s) 27, 153, 370
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 376, 383
Bse1I ACTGG 1 cut(s) 459
Bse21I CCTNAGG 1 cut(s) 229
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 3 cut(s) 27, 153, 370
BseLI CCNNNNNNNGG 3 cut(s) 154, 376, 383
BseMII CTCAG 6 cut(s) 104, 113, 218, 243, 323, 429
BseNI ACTGG 1 cut(s) 459
BseX3I CGGCCG 1 cut(s) 303
BseXI GCAGC 1 cut(s) 155
Bsh1236I CGCG 1 cut(s) 155
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 1 cut(s) 305
BshNI GGYRCC 1 cut(s) 455
BshVI ATCGAT 1 cut(s) 66
BsiEI CGRYCG 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 1 cut(s) 377
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 3 cut(s) 154, 376, 383
BsmAI GTCTC 2 cut(s) 288, 428
BsmBI CGTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 1 cut(s) 305
BsoBI CYCGRG 1 cut(s) 147
Bsp1720I GCTNAGC 1 cut(s) 415
Bsp19I CCATGG 1 cut(s) 370
BspACI CCGC 6 cut(s) 78, 153, 155, 306, 314, 486
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 6 cut(s) 103, 112, 217, 242, 322, 428
BspDI ATCGAT 1 cut(s) 66
BspFNI CGCG 1 cut(s) 155
BspLI GGNNCC 1 cut(s) 457
BspMI ACCTGC 1 cut(s) 388
BspT107I GGYRCC 1 cut(s) 455
BsrBI CCGCTC 1 cut(s) 308
BsrI ACTGG 1 cut(s) 459
BssECI CCNNGG 3 cut(s) 27, 153, 370
BssSI CACGAG 1 cut(s) 364
BssT1I CCWWGG 2 cut(s) 27, 370
Bst2BI CACGAG 1 cut(s) 364
Bst4CI ACNGT 1 cut(s) 199
BstC8I GCNNGC 1 cut(s) 490
BstDEI CTNAG 9 cut(s) 90, 99, 128, 173, 204, 229, 309, 415, 441
BstDSI CCRYGG 2 cut(s) 153, 370
BstFNI CGCG 1 cut(s) 155
BstMAI GTCTC 2 cut(s) 288, 428
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 4 cut(s) 149, 152, 200, 311
BstNSI RCATGY 1 cut(s) 301
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 1 cut(s) 155
BstZI CGGCCG 1 cut(s) 303
Bsu15I ATCGAT 1 cut(s) 66
Bsu36I CCTNAGG 1 cut(s) 229
BsuI GTATCC 1 cut(s) 105
BsuRI GGCC 1 cut(s) 305
BsuTUI ATCGAT 1 cut(s) 66
BtgI CCRYGG 2 cut(s) 153, 370
BtgZI GCGATG 1 cut(s) 335
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 2 cut(s) 195, 295
BveI ACCTGC 1 cut(s) 388
Cac8I GCNNGC 1 cut(s) 490
Cfr13I GGNCC 3 cut(s) 233, 260, 374
Cfr42I CCGCGG 1 cut(s) 156
ClaI ATCGAT 1 cut(s) 66
Csp6I GTAC 2 cut(s) 329, 343
CviAII CATG 5 cut(s) 107, 237, 298, 332, 371
CviQI GTAC 2 cut(s) 329, 343
DdeI CTNAG 9 cut(s) 90, 99, 128, 173, 204, 229, 309, 415, 441
EaeI YGGCCR 1 cut(s) 303
EagI CGGCCG 1 cut(s) 303
EclXI CGGCCG 1 cut(s) 303
Eco130I CCWWGG 2 cut(s) 27, 370
Eco47I GGWCC 3 cut(s) 233, 260, 374
Eco52I CGGCCG 1 cut(s) 303
Eco81I CCTNAGG 1 cut(s) 229
Eco88I CYCGRG 1 cut(s) 147
EcoT14I CCWWGG 2 cut(s) 27, 370
ErhI CCWWGG 2 cut(s) 27, 370
Esp3I CGTCTC 1 cut(s) 428
FaeI CATG 5 cut(s) 110, 240, 301, 335, 374
FaqI GGGAC 1 cut(s) 88
FatI CATG 5 cut(s) 106, 236, 297, 331, 370
FauI CCCGC 1 cut(s) 493
FauNDI CATATG 1 cut(s) 265
Fnu4HI GCNGC 3 cut(s) 144, 153, 306
Fsp4HI GCNGC 3 cut(s) 144, 153, 306
FspBI CTAG 2 cut(s) 224, 507
GluI GCNGC 3 cut(s) 144, 153, 306
HaeIII GGCC 1 cut(s) 305
HapII CCGG 1 cut(s) 377
Hin1II CATG 5 cut(s) 110, 240, 301, 335, 374
HinfI GANTC 1 cut(s) 274
HpaII CCGG 1 cut(s) 377
Hpy166II GTNNAC 1 cut(s) 386
Hpy188III TCNNGA 4 cut(s) 116, 206, 245, 258
Hpy8I GTNNAC 1 cut(s) 386
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4V TGCA 2 cut(s) 240, 281
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 152, 200, 311
HpyF3I CTNAG 9 cut(s) 90, 99, 128, 173, 204, 229, 309, 415, 441
Hsp92II CATG 5 cut(s) 110, 240, 301, 335, 374
KspI CCGCGG 1 cut(s) 156
LmnI GCTCC 1 cut(s) 503
LpnPI CCDG 9 cut(s) 129, 191, 216, 243, 296, 383, 390, 472, 474
Lsp1109I GCAGC 1 cut(s) 155
MaeI CTAG 2 cut(s) 224, 507
MaeIII GTNAC 1 cut(s) 473
MbiI CCGCTC 1 cut(s) 308
MluCI AATT 2 cut(s) 49, 493
MnlI CCTC 5 cut(s) 85, 108, 180, 238, 265
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 1 cut(s) 377
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 4 cut(s) 149, 152, 200, 311
NcoI CCATGG 1 cut(s) 370
NdeI CATATG 1 cut(s) 265
NlaIII CATG 5 cut(s) 110, 240, 301, 335, 374
NlaIV GGNNCC 1 cut(s) 457
NspI RCATGY 1 cut(s) 301
PciI ACATGT 1 cut(s) 297
PfeI GAWTC 1 cut(s) 274
PkrI GCNGC 3 cut(s) 145, 154, 307
PscI ACATGT 1 cut(s) 297
PspN4I GGNNCC 1 cut(s) 457
PspPI GGNCC 3 cut(s) 233, 260, 374
RsaI GTAC 2 cut(s) 330, 344
RsaNI GTAC 2 cut(s) 329, 343
SacII CCGCGG 1 cut(s) 156
SatI GCNGC 3 cut(s) 144, 153, 306
Sau96I GGNCC 3 cut(s) 233, 260, 374
SetI ASST 8 cut(s) 15, 29, 125, 129, 235, 402, 421, 508
Sfr303I CCGCGG 1 cut(s) 156
SgrBI CCGCGG 1 cut(s) 156
SinI GGWCC 3 cut(s) 233, 260, 374
Sse9I AATT 2 cut(s) 49, 493
SsiI CCGC 6 cut(s) 78, 153, 155, 306, 314, 486
SspMI CTAG 2 cut(s) 224, 507
StyI CCWWGG 2 cut(s) 27, 370
TaaI ACNGT 1 cut(s) 199
TaqI TCGA 1 cut(s) 66
TasI AATT 2 cut(s) 49, 493
TauI GCSGC 2 cut(s) 155, 308
TfiI GAWTC 1 cut(s) 274
TscAI CASTG 2 cut(s) 202, 295
TseI GCWGC 1 cut(s) 143
TspDTI ATGAA 1 cut(s) 123
TspRI CASTG 2 cut(s) 202, 295
VpaK11BI GGWCC 3 cut(s) 233, 260, 374
XceI RCATGY 1 cut(s) 301
XspI CTAG 2 cut(s) 224, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.