AT5G01800

Saposin (B) Domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001255
Physical Location & Seq
Forward (+)
306939 .. 309289
2351 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G01800.1

Sequence Viewer

Length: 654 bp
ATGGGCGGTAGATTTGGAGTCCTCCTAGTCCTCTTCCTCTTGAGCTGGTCTTGTCATGCTACAAACCCGATTCTTCTCGAACCATTCGAATCAGCTCATGATGACAACCAAGTCTGTGAACTATGTGACAAGTATGTCACGCTAGTCATTGATTACCTTCAAGACTACGACAACCAGAATGAACTCGTTGAGGCCCTTCATATCAGCTGCTCTCAGATTCCTCCTCTCAAAAAACAGTGTCTTTCAATGGTAGATCATTACACCCAACTTTTCTTCACACAAGTCTCTACTATCAAATCAGACCAAATCTGCAAACGGCTTAACCTCTGTCAAGCTGTGACACCGGCCTTTGCCTCTCAAGTCCATCAAGGAAACTGCGAGGCTTGCCGCGAGACTGTCTCTGAGGTTGTCACCAAACTCAAGGATCCTGAGACTAAGCTGAAGATAATTCGGTTACTTCTCAAGGAATGCAAGTCGCTTAACAATTACCAGGACAAGTGCAAGAAGATGGTATTCGAGTACGGGCCTCTGATGCTTACGGATTTGCAGAAGTTTCTGGAGAAGAAAGACGTGTGCACTATCCTCCACGTCTGTCCAGGTCCAGCCACCCACCGTGACTACGTTCCTGCGGTGGAGTCATTGGCAGATTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0001655 GO:0001664 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0002576 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005102 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005766 GO:0005773 GO:0005774 GO:0005775 GO:0005886 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006687 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006887 GO:0006955 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0007193 GO:0007275 GO:0008047 GO:0008150 GO:0008152 GO:0008289 GO:0009888 GO:0009894 GO:0009987 GO:0010506 GO:0010876 GO:0012505 GO:0012506 GO:0015711 GO:0015849 GO:0015925 GO:0016020 GO:0016192 GO:0016787 GO:0016798 GO:0019216 GO:0019222 GO:0022414 GO:0023052 GO:0030141 GO:0030154 GO:0030234 GO:0030659 GO:0030667 GO:0030850 GO:0030855 GO:0031090 GO:0031323 GO:0031329 GO:0031406 GO:0031410 GO:0031974 GO:0031982 GO:0032501 GO:0032502 GO:0032940 GO:0033036 GO:0033218 GO:0033293 GO:0035265 GO:0035577 GO:0035594 GO:0035627 GO:0036094 GO:0036230 GO:0040007 GO:0042119 GO:0042582 GO:0042802 GO:0042803 GO:0042886 GO:0043085 GO:0043167 GO:0043168 GO:0043177 GO:0043202 GO:0043208 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0044093 GO:0044237 GO:0044238 GO:0044255 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046625 GO:0046836 GO:0046903 GO:0046942 GO:0046983 GO:0048513 GO:0048589 GO:0048608 GO:0048731 GO:0048732 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0051861 GO:0060429 GO:0060736 GO:0060742 GO:0061458 GO:0065007 GO:0065009 GO:0070013 GO:0071702 GO:0071704 GO:0071705 GO:0071944 GO:0080090 GO:0097001 GO:0097367 GO:0097708 GO:0098588 GO:0098772 GO:0098805 GO:0098852 GO:0099503 GO:1901135 GO:1901264 GO:1901564 GO:1903509 GO:1905572 GO:1905573 GO:1905574 GO:1905575 GO:1905576 GO:1905577
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
SapB_1 SapB_2

Protein Analysis

217

Amino Acids

24.63

Weight (kDa)

5.79

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SapB_1 PF05184 38 - 74 1e-06 Saposin-like type B, region 1
SapB_1 PF05184 125 - 160 1.7e-07 Saposin-like type B, region 1
SapB_2 PF03489 165 - 198 3.8e-09 Saposin-like type B, region 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 110, 134
AccII CGCG 1 cut(s) 390
AciI CCGC 3 cut(s) 6, 388, 629
AclWI GGATC 2 cut(s) 419, 432
AcuI CTGAAG 1 cut(s) 461
AfaI GTAC 1 cut(s) 521
AflIII ACRYGT 1 cut(s) 570
AgsI TTSAA 2 cut(s) 161, 246
AjiI CACGTC 2 cut(s) 571, 589
AjnI CCWGG 2 cut(s) 489, 595
AluBI AGCT 5 cut(s) 45, 95, 207, 335, 439
AluI AGCT 5 cut(s) 45, 95, 207, 335, 439
Alw21I GWGCWC 1 cut(s) 578
Alw26I GTCTC 4 cut(s) 289, 386, 403, 425
Alw44I GTGCAC 1 cut(s) 574
AlwI GGATC 2 cut(s) 419, 432
AoxI GGCC 3 cut(s) 192, 345, 524
ApaLI GTGCAC 1 cut(s) 574
ApeKI GCWGC 1 cut(s) 207
AspS9I GGNCC 3 cut(s) 193, 524, 599
AsuHPI GGTGA 1 cut(s) 403
AsuII TTCGAA 1 cut(s) 87
AvaII GGWCC 1 cut(s) 599
BaeGI GKGCMC 1 cut(s) 578
BamHI GGATCC 1 cut(s) 424
Bbv12I GWGCWC 1 cut(s) 578
BbvI GCAGC 1 cut(s) 194
BccI CCATC 2 cut(s) 372, 502
BceAI ACGGC 1 cut(s) 332
BciT130I CCWGG 2 cut(s) 491, 597
BcoDI GTCTC 4 cut(s) 289, 386, 403, 425
BfaI CTAG 2 cut(s) 26, 143
BisI GCNGC 2 cut(s) 208, 388
BlsI GCNGC 2 cut(s) 209, 389
Bme1390I CCNGG 2 cut(s) 491, 597
Bme18I GGWCC 1 cut(s) 599
BmgBI CACGTC 2 cut(s) 571, 589
BmgT120I GGNCC 3 cut(s) 193, 524, 599
BmiI GGNNCC 1 cut(s) 426
BmrFI CCNGG 2 cut(s) 491, 597
BmsI GCATC 1 cut(s) 522
BplI GAGNNNNNCTC 2 cut(s) 383, 415
BpmI CTGGAG 1 cut(s) 578
Bpu14I TTCGAA 1 cut(s) 87
BpuEI CTTGAG 4 cut(s) 61, 342, 404, 446
Bse118I RCCGGY 1 cut(s) 343
BseBI CCWGG 2 cut(s) 491, 597
BseMII CTCAG 3 cut(s) 227, 393, 420
BseRI GAGGAG 1 cut(s) 213
BseSI GKGCMC 1 cut(s) 578
BseXI GCAGC 1 cut(s) 194
Bsh1236I CGCG 1 cut(s) 390
BshFI GGCC 3 cut(s) 194, 347, 526
BsiHKAI GWGCWC 1 cut(s) 578
BsiSI CCGG 1 cut(s) 344
BsmAI GTCTC 4 cut(s) 289, 386, 403, 425
BsmI GAATGC 1 cut(s) 473
BsnI GGCC 3 cut(s) 194, 347, 526
Bsp119I TTCGAA 1 cut(s) 87
Bsp1286I GDGCHC 1 cut(s) 578
Bsp143I GATC 2 cut(s) 253, 424
BspACI CCGC 3 cut(s) 6, 388, 629
BspANI GGCC 3 cut(s) 194, 347, 526
BspCNI CTCAG 3 cut(s) 226, 394, 421
BspFNI CGCG 1 cut(s) 390
BspHI TCATGA 1 cut(s) 97
BspLI GGNNCC 1 cut(s) 426
BspPI GGATC 2 cut(s) 419, 432
BspT104I TTCGAA 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 343
BssAI RCCGGY 1 cut(s) 343
BssMI GATC 2 cut(s) 253, 424
Bst2UI CCWGG 2 cut(s) 491, 597
Bst4CI ACNGT 3 cut(s) 237, 397, 614
Bst6I CTCTTC 1 cut(s) 38
BstBI TTCGAA 1 cut(s) 87
BstC8I GCNNGC 1 cut(s) 385
BstDEI CTNAG 4 cut(s) 213, 402, 429, 435
BstFNI CGCG 1 cut(s) 390
BstKTI GATC 2 cut(s) 256, 427
BstMAI GTCTC 4 cut(s) 289, 386, 403, 425
BstMBI GATC 2 cut(s) 253, 424
BstMWI GCNNNNNNNGC 2 cut(s) 384, 532
BstNI CCWGG 2 cut(s) 491, 597
BstSCI CCNGG 2 cut(s) 489, 595
BstSLI GKGCMC 1 cut(s) 578
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 1 cut(s) 194
BstX2I RGATCY 1 cut(s) 424
BstYI RGATCY 1 cut(s) 424
BsuRI GGCC 3 cut(s) 194, 347, 526
BtrI CACGTC 2 cut(s) 571, 589
BtsIMutI CAGTG 1 cut(s) 242
Cac8I GCNNGC 1 cut(s) 385
CciI TCATGA 1 cut(s) 97
Cfr10I RCCGGY 1 cut(s) 343
Cfr13I GGNCC 3 cut(s) 193, 524, 599
Csp6I GTAC 1 cut(s) 520
CviAII CATG 2 cut(s) 56, 98
CviQI GTAC 1 cut(s) 520
DdeI CTNAG 4 cut(s) 213, 402, 429, 435
DpnI GATC 2 cut(s) 255, 426
DpnII GATC 2 cut(s) 253, 424
DrdI GACNNNNNNGTC 2 cut(s) 110, 134
DseDI GACNNNNNNGTC 2 cut(s) 110, 134
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 599
Eco57I CTGAAG 1 cut(s) 461
EcoO109I RGGNCCY 1 cut(s) 193
EcoRII CCWGG 2 cut(s) 489, 595
FaeI CATG 2 cut(s) 59, 101
FaiI YATR 5 cut(s) 57, 99, 124, 135, 201
FatI CATG 2 cut(s) 55, 97
Fnu4HI GCNGC 2 cut(s) 208, 388
Fsp4HI GCNGC 2 cut(s) 208, 388
FspBI CTAG 2 cut(s) 26, 143
GluI GCNGC 2 cut(s) 208, 388
GsuI CTGGAG 1 cut(s) 578
HaeIII GGCC 3 cut(s) 194, 347, 526
HapII CCGG 1 cut(s) 344
Hin1II CATG 2 cut(s) 59, 101
HinfI GANTC 6 cut(s) 18, 70, 89, 217, 635, 647
HpaII CCGG 1 cut(s) 344
HphI GGTGA 1 cut(s) 403
Hpy166II GTNNAC 2 cut(s) 119, 576
Hpy188I TCNGA 4 cut(s) 216, 301, 403, 531
Hpy188III TCNNGA 6 cut(s) 40, 77, 98, 161, 428, 557
Hpy8I GTNNAC 2 cut(s) 119, 576
HpyAV CCTTC 2 cut(s) 167, 206
HpyCH4III ACNGT 3 cut(s) 237, 397, 614
HpyCH4IV ACGT 3 cut(s) 570, 588, 621
HpyCH4V TGCA 5 cut(s) 312, 471, 501, 547, 576
HpyF10VI GCNNNNNNNGC 2 cut(s) 384, 532
HpyF3I CTNAG 4 cut(s) 213, 402, 429, 435
HpySE526I ACGT 3 cut(s) 570, 588, 621
Hsp92II CATG 2 cut(s) 59, 101
Kzo9I GATC 2 cut(s) 253, 424
Lsp1109I GCAGC 1 cut(s) 194
LweI GCATC 1 cut(s) 522
MaeI CTAG 2 cut(s) 26, 143
MaeII ACGT 3 cut(s) 570, 588, 621
MaeIII GTNAC 6 cut(s) 125, 136, 337, 409, 453, 614
MalI GATC 2 cut(s) 255, 426
MboI GATC 2 cut(s) 253, 424
MboII GAAGA 6 cut(s) 25, 65, 265, 454, 517, 574
MflI RGATCY 1 cut(s) 424
MhlI GDGCHC 1 cut(s) 578
MluCI AATT 2 cut(s) 447, 484
MlyI GAGTC 2 cut(s) 27, 644
MseI TTAA 2 cut(s) 321, 480
MspA1I CMGCKG 1 cut(s) 207
MspI CCGG 1 cut(s) 344
MspR9I CCNGG 2 cut(s) 491, 597
Mva1269I GAATGC 1 cut(s) 473
MvaI CCWGG 2 cut(s) 491, 597
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 2 cut(s) 384, 532
NdeII GATC 2 cut(s) 253, 424
NlaIII CATG 2 cut(s) 59, 101
NlaIV GGNNCC 1 cut(s) 426
NmuCI GTSAC 5 cut(s) 125, 136, 337, 409, 614
NspV TTCGAA 1 cut(s) 87
PagI TCATGA 1 cut(s) 97
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PctI GAATGC 1 cut(s) 473
PfeI GAWTC 4 cut(s) 70, 89, 217, 647
PkrI GCNGC 2 cut(s) 209, 389
PleI GAGTC 2 cut(s) 26, 643
PpsI GAGTC 2 cut(s) 26, 643
Psp6I CCWGG 2 cut(s) 489, 595
PspGI CCWGG 2 cut(s) 489, 595
PspN4I GGNNCC 1 cut(s) 426
PspPI GGNCC 3 cut(s) 193, 524, 599
PsuI RGATCY 1 cut(s) 424
PvuII CAGCTG 1 cut(s) 207
RsaI GTAC 1 cut(s) 521
RsaNI GTAC 1 cut(s) 520
SaqAI TTAA 2 cut(s) 321, 480
SatI GCNGC 2 cut(s) 208, 388
Sau3AI GATC 2 cut(s) 253, 424
Sau96I GGNCC 3 cut(s) 193, 524, 599
SchI GAGTC 2 cut(s) 27, 644
ScrFI CCNGG 2 cut(s) 491, 597
SduI GDGCHC 1 cut(s) 578
SfaNI GCATC 1 cut(s) 522
SfuI TTCGAA 1 cut(s) 87
SinI GGWCC 1 cut(s) 599
SmlI CTYRAG 4 cut(s) 40, 357, 419, 461
SmoI CTYRAG 4 cut(s) 40, 357, 419, 461
Sse9I AATT 2 cut(s) 447, 484
SsiI CCGC 3 cut(s) 6, 388, 629
SspMI CTAG 2 cut(s) 26, 143
StyD4I CCNGG 2 cut(s) 489, 595
TaaI ACNGT 3 cut(s) 237, 397, 614
TaiI ACGT 3 cut(s) 573, 591, 624
TaqI TCGA 3 cut(s) 78, 87, 516
TasI AATT 2 cut(s) 447, 484
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 4 cut(s) 70, 89, 217, 647
Tru1I TTAA 2 cut(s) 321, 480
Tru9I TTAA 2 cut(s) 321, 480
TscAI CASTG 1 cut(s) 242
TseFI GTSAC 5 cut(s) 125, 136, 337, 409, 614
TseI GCWGC 1 cut(s) 207
Tsp45I GTSAC 5 cut(s) 125, 136, 337, 409, 614
TspDTI ATGAA 2 cut(s) 188, 195
TspGWI ACGGA 1 cut(s) 554
TspRI CASTG 1 cut(s) 242
VneI GTGCAC 1 cut(s) 574
VpaK11BI GGWCC 1 cut(s) 599
XspI CTAG 2 cut(s) 26, 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.