AT5G14280

TRAM, LAG1 and CLN8 homology domains.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001485
Physical Location & Seq
Forward (+)
4608745 .. 4610448
1704 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G14280.1

Sequence Viewer

Length: 906 bp
ATGGCGACTCCGACGGAGCTAGGTTTCTCTTCACCTGGTGGTGGTGGTGACGACTCTGACGATAACCCTCCACAGAAGCGTACTTCAAAACGTACTGCTTCCGAAACAGCAACAGAAGAAGAAACGAAGAAGAAGAAAAAGAAGAAGACGAAGCACAACACAAAAATGGCTTCTCCTCCGAGCAACCGGATCTGGAACGAAGAAGACGAGCTCTCTATCTTAAAGGGATTGGTGGATTTTCGAGCTAAAACAGGACTCGAATCCAAAATTGATTGGGATGCGTTTTATTGTTACGTCAAAGGTTCCATTCATGTGAAAGTTTCCAAGTGCCAGCTTATGAGTAAGACCAGGAAGTTGAAGAAGAAGTTCCTTGATCAAATGGAGAAAATCGATCAAGGAAATGATCCACATTTTACTAGGTCTAGTGAAACTGAAGCTTTTGGATATTCCATGATGATTTGGCGTAAAATTGATGCTGAATACACTAATGGTGTTATGGATAAAGCACATCAAAGCGAGAGTGGAGAGGAGGTGTTTGAGGAGGATGAGGAAGTGGCCTTAATTGACAAAGGGGCTGCGAAAAGTGGCAAGTCACCTCATGAAGCTGTTGTTGTTGTTGACAAAATAACTACGAAGAAGAACGGGACTGCTGGAAAAGAGAGTGATGATGATGATGATGATGTGTTGTGTGCTGTGCGGGATGCGTTTGAGACCACCATGATGTCGCAAGGATTAAGTGATTATCAAAAGAAGTTGCAGCTTGAGAAGCTGATGAACCTTGGAACTGGTAAAAGAAGAGAGTTGAGCAATGAATGGAAAGCACTATGTGTTGAGGAACTAAAATTGAATATCAACAAGCTTCGATTTTCCGCCAAGCTTGCAGAGGCAGCAAATGATGGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573 TRAM_LAG1_CLN8

Protein Analysis

301

Amino Acids

33.72

Weight (kDa)

6.3

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 62 - 154 3.5e-28 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 697, 870
AclWI GGATC 2 cut(s) 197, 398
AcuI CTGAAG 1 cut(s) 453
AdeI CACNNNGTG 2 cut(s) 38, 827
AfaI GTAC 2 cut(s) 82, 94
AfiI CCNNNNNNNGG 1 cut(s) 41
AgsI TTSAA 3 cut(s) 87, 358, 847
AjnI CCWGG 2 cut(s) 34, 347
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 1 cut(s) 704
AlwI GGATC 2 cut(s) 197, 398
AoxI GGCC 1 cut(s) 555
ApeKI GCWGC 3 cut(s) 575, 757, 887
Asp700I GAANNNNTTC 1 cut(s) 365
AsuHPI GGTGA 3 cut(s) 24, 59, 585
BanII GRGCYC 1 cut(s) 213
BbsI GAAGAC 2 cut(s) 152, 210
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 3 cut(s) 562, 769, 899
BccI CCATC 1 cut(s) 890
BciT130I CCWGG 2 cut(s) 36, 349
BclI TGATCA 1 cut(s) 373
BcoDI GTCTC 1 cut(s) 704
BfaI CTAG 3 cut(s) 20, 417, 423
BisI GCNGC 3 cut(s) 576, 758, 888
BlsI GCNGC 3 cut(s) 577, 759, 889
Bme1390I CCNGG 2 cut(s) 36, 349
BmiI GGNNCC 1 cut(s) 304
BmrFI CCNGG 2 cut(s) 36, 349
BmsI GCATC 3 cut(s) 268, 463, 691
BpiI GAAGAC 2 cut(s) 152, 210
BpuEI CTTGAG 1 cut(s) 782
Bsa29I ATCGAT 1 cut(s) 390
BsaI GGTCTC 1 cut(s) 704
BsaJI CCNNGG 1 cut(s) 778
BsaWI WCCGGW 1 cut(s) 186
BsaXI ACNNNNNCTCC 2 cut(s) 516, 546
Bsc4I CCNNNNNNNGG 1 cut(s) 41
Bse1I ACTGG 1 cut(s) 790
Bse3DI GCAATG 1 cut(s) 814
BseBI CCWGG 2 cut(s) 36, 349
BseCI ATCGAT 1 cut(s) 390
BseDI CCNNGG 1 cut(s) 778
BseGI GGATG 3 cut(s) 283, 550, 706
BseLI CCNNNNNNNGG 1 cut(s) 41
BseMI GCAATG 1 cut(s) 814
BseNI ACTGG 1 cut(s) 790
BseRI GAGGAG 3 cut(s) 165, 542, 554
BseXI GCAGC 3 cut(s) 562, 769, 899
BshFI GGCC 1 cut(s) 557
BshVI ATCGAT 1 cut(s) 390
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 1 cut(s) 187
BslFI GGGAC 1 cut(s) 658
BslI CCNNNNNNNGG 1 cut(s) 41
BsmAI GTCTC 1 cut(s) 704
BsmFI GGGAC 1 cut(s) 658
BsnI GGCC 1 cut(s) 557
Bso31I GGTCTC 1 cut(s) 704
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 4 cut(s) 189, 373, 391, 403
BspACI CCGC 2 cut(s) 697, 870
BspANI GGCC 1 cut(s) 557
BspDI ATCGAT 1 cut(s) 390
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 1 cut(s) 304
BspPI GGATC 2 cut(s) 197, 398
BspTNI GGTCTC 1 cut(s) 704
BsrDI GCAATG 1 cut(s) 814
BsrI ACTGG 1 cut(s) 790
BssECI CCNNGG 1 cut(s) 778
BssMI GATC 4 cut(s) 189, 373, 391, 403
BssT1I CCWWGG 1 cut(s) 778
Bst2UI CCWGG 2 cut(s) 36, 349
Bst6I CTCTTC 2 cut(s) 34, 790
BstC8I GCNNGC 2 cut(s) 332, 879
BstF5I GGATG 3 cut(s) 283, 550, 706
BstKTI GATC 4 cut(s) 192, 376, 394, 406
BstMAI GTCTC 1 cut(s) 704
BstMBI GATC 4 cut(s) 189, 373, 391, 403
BstMWI GCNNNNNNNGC 3 cut(s) 766, 878, 887
BstNI CCWGG 2 cut(s) 36, 349
BstSCI CCNGG 2 cut(s) 34, 347
BstV1I GCAGC 3 cut(s) 562, 769, 899
BstV2I GAAGAC 2 cut(s) 152, 210
BstX2I RGATCY 1 cut(s) 189
BstYI RGATCY 1 cut(s) 189
Bsu15I ATCGAT 1 cut(s) 390
BsuRI GGCC 1 cut(s) 557
BsuTUI ATCGAT 1 cut(s) 390
BtsCI GGATG 3 cut(s) 283, 550, 706
Cac8I GCNNGC 2 cut(s) 332, 879
CciI TCATGA 1 cut(s) 598
ClaI ATCGAT 1 cut(s) 390
CsiI ACCWGGT 1 cut(s) 34
Csp6I GTAC 2 cut(s) 81, 93
CviAII CATG 4 cut(s) 311, 451, 599, 718
CviQI GTAC 2 cut(s) 81, 93
DpnI GATC 4 cut(s) 191, 375, 393, 405
DpnII GATC 4 cut(s) 189, 373, 391, 403
DraIII CACNNNGTG 2 cut(s) 38, 827
Eam1104I CTCTTC 2 cut(s) 34, 790
EarI CTCTTC 2 cut(s) 34, 790
EciI GGCGGA 1 cut(s) 859
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 778
Eco24I GRGCYC 1 cut(s) 213
Eco31I GGTCTC 1 cut(s) 704
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 453
EcoICRI GAGCTC 1 cut(s) 211
EcoRII CCWGG 2 cut(s) 34, 347
EcoT14I CCWWGG 1 cut(s) 778
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 778
FaeI CATG 4 cut(s) 314, 454, 602, 721
FaiI YATR 7 cut(s) 312, 338, 452, 497, 600, 719, 826
FaqI GGGAC 1 cut(s) 658
FatI CATG 4 cut(s) 310, 450, 598, 717
FauI CCCGC 1 cut(s) 690
FbaI TGATCA 1 cut(s) 373
Fnu4HI GCNGC 3 cut(s) 576, 758, 888
FokI GGATG 3 cut(s) 290, 557, 713
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 3 cut(s) 576, 758, 888
FspBI CTAG 3 cut(s) 20, 417, 423
GluI GCNGC 3 cut(s) 576, 758, 888
HaeIII GGCC 1 cut(s) 557
HapII CCGG 1 cut(s) 187
Hin1II CATG 4 cut(s) 314, 454, 602, 721
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HindIII AAGCTT 3 cut(s) 435, 857, 875
HinfI GANTC 4 cut(s) 7, 53, 255, 260
HpaII CCGG 1 cut(s) 187
HphI GGTGA 3 cut(s) 24, 59, 585
Hpy166II GTNNAC 1 cut(s) 619
Hpy188I TCNGA 4 cut(s) 12, 58, 103, 180
Hpy188III TCNNGA 2 cut(s) 193, 599
Hpy8I GTNNAC 1 cut(s) 619
Hpy99I CGWCG 1 cut(s) 16
HpyCH4IV ACGT 2 cut(s) 91, 294
HpyCH4V TGCA 2 cut(s) 757, 881
HpyF10VI GCNNNNNNNGC 3 cut(s) 766, 878, 887
HpySE526I ACGT 2 cut(s) 91, 294
Hsp92II CATG 4 cut(s) 314, 454, 602, 721
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 4 cut(s) 189, 373, 391, 403
LmnI GCTCC 1 cut(s) 16
Lsp1109I GCAGC 3 cut(s) 562, 769, 899
LweI GCATC 3 cut(s) 268, 463, 691
MabI ACCWGGT 1 cut(s) 34
MaeI CTAG 3 cut(s) 20, 417, 423
MaeII ACGT 2 cut(s) 91, 294
MaeIII GTNAC 3 cut(s) 47, 290, 591
MalI GATC 4 cut(s) 191, 375, 393, 405
MboI GATC 4 cut(s) 189, 373, 391, 403
MflI RGATCY 1 cut(s) 189
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 4 cut(s) 267, 468, 561, 842
MlyI GAGTC 2 cut(s) 47, 249
MmeI TCCRAC 1 cut(s) 35
MroXI GAANNNNTTC 1 cut(s) 365
MseI TTAA 3 cut(s) 221, 560, 734
MslI CAYNNNNRTG 3 cut(s) 164, 311, 719
MspI CCGG 1 cut(s) 187
MspR9I CCNGG 2 cut(s) 36, 349
MvaI CCWGG 2 cut(s) 36, 349
MwoI GCNNNNNNNGC 3 cut(s) 766, 878, 887
NdeII GATC 4 cut(s) 189, 373, 391, 403
NlaIII CATG 4 cut(s) 314, 454, 602, 721
NlaIV GGNNCC 1 cut(s) 304
NmuCI GTSAC 2 cut(s) 47, 591
PagI TCATGA 1 cut(s) 598
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 204
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 3 cut(s) 577, 759, 889
PleI GAGTC 2 cut(s) 47, 249
PpsI GAGTC 2 cut(s) 47, 249
Psp124BI GAGCTC 1 cut(s) 213
Psp6I CCWGG 2 cut(s) 34, 347
PspGI CCWGG 2 cut(s) 34, 347
PspN4I GGNNCC 1 cut(s) 304
PsuI RGATCY 1 cut(s) 189
RsaI GTAC 2 cut(s) 82, 94
RsaNI GTAC 2 cut(s) 81, 93
RseI CAYNNNNRTG 3 cut(s) 164, 311, 719
SacI GAGCTC 1 cut(s) 213
SaqAI TTAA 3 cut(s) 221, 560, 734
SatI GCNGC 3 cut(s) 576, 758, 888
Sau3AI GATC 4 cut(s) 189, 373, 391, 403
SchI GAGTC 2 cut(s) 47, 249
ScrFI CCNGG 2 cut(s) 36, 349
SduI GDGCHC 1 cut(s) 213
SexAI ACCWGGT 1 cut(s) 34
SfaNI GCATC 3 cut(s) 268, 463, 691
SmiMI CAYNNNNRTG 3 cut(s) 164, 311, 719
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
Sse9I AATT 4 cut(s) 267, 468, 561, 842
SsiI CCGC 2 cut(s) 697, 870
SspMI CTAG 3 cut(s) 20, 417, 423
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 2 cut(s) 34, 347
StyI CCWWGG 1 cut(s) 778
TaiI ACGT 2 cut(s) 94, 297
TaqI TCGA 4 cut(s) 241, 258, 390, 862
TasI AATT 4 cut(s) 267, 468, 561, 842
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 3 cut(s) 221, 560, 734
Tru9I TTAA 3 cut(s) 221, 560, 734
TseFI GTSAC 2 cut(s) 47, 591
TseI GCWGC 3 cut(s) 575, 757, 887
Tsp45I GTSAC 2 cut(s) 47, 591
TspDTI ATGAA 4 cut(s) 299, 615, 788, 825
TspGWI ACGGA 1 cut(s) 29
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 3 cut(s) 20, 417, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.