FvH4_2g04540

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001292
Physical Location & Seq
Forward (+)
3496208 .. 3496612
405 bp
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UTR
Exon/CDS
Intron
FvH4_2g04540.t1

Sequence Viewer

Length: 405 bp
ATGCCTAAAAGGATTTTCGTCTTCACCTACAATGATTTTGTCACTGCCTCGAAAAACAACTGGAATGAAGAATACAAGGAAGCTTGGGCTAGTTACAGAAAGAGAGGGTTAACTGTGCCGAAAATGGTGTTTTGGAATCTTAAAGACCCGATTGCAGAGCCAGAGGTGTTTGGGAGCCCTGTGAAGAACCAAAATGAGGGGATGATCATGACTGGTTTTTCAAACCTTCTGCTCAAATTGTTCTTCAGAGGAGAGATGGATTCGAGATCGAATGCAGGCCAAAGCCATGCTTTTTATCGTGGTGTTAATGTGCCGTTGGTGATGAAATTTGTTCCAAAGGTAGAGCATGTGATGAATTGTGCACTTTCAAGAGAGGAGTTGAACAATCTTGTTGTGTTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

135

Amino Acids

15.51

Weight (kDa)

9.07

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7788 PF25043 1 - 89 1.4e-15 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 326
AcuI CTGAAG 1 cut(s) 229
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 3 cut(s) 222, 369, 382
AluBI AGCT 1 cut(s) 83
AluI AGCT 1 cut(s) 83
Alw21I GWGCWC 1 cut(s) 364
Alw44I GTGCAC 1 cut(s) 360
AoxI GGCC 1 cut(s) 277
ApaLI GTGCAC 1 cut(s) 360
ApoI RAATTY 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 16, 331
BaeGI GKGCMC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 179
BbsI GAAGAC 1 cut(s) 13
Bbv12I GWGCWC 1 cut(s) 364
BccI CCATC 1 cut(s) 250
BceAI ACGGC 1 cut(s) 298
BclI TGATCA 1 cut(s) 204
BfaI CTAG 1 cut(s) 90
BmiI GGNNCC 1 cut(s) 176
BpiI GAAGAC 1 cut(s) 13
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 2 cut(s) 65, 217
BseGI GGATG 1 cut(s) 207
BseLI CCNNNNNNNGG 1 cut(s) 196
BseNI ACTGG 2 cut(s) 65, 217
BseRI GAGGAG 2 cut(s) 264, 389
BseSI GKGCMC 1 cut(s) 364
BshFI GGCC 1 cut(s) 279
BsiHKAI GWGCWC 1 cut(s) 364
BslI CCNNNNNNNGG 1 cut(s) 196
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 1 cut(s) 279
Bsp1286I GDGCHC 2 cut(s) 179, 364
Bsp143I GATC 2 cut(s) 204, 266
BspANI GGCC 1 cut(s) 279
BspHI TCATGA 1 cut(s) 207
BspLI GGNNCC 1 cut(s) 176
BsrI ACTGG 2 cut(s) 65, 217
BssMI GATC 2 cut(s) 204, 266
Bst4CI ACNGT 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 277
BstF5I GGATG 1 cut(s) 207
BstKTI GATC 2 cut(s) 207, 269
BstMBI GATC 2 cut(s) 204, 266
BstNSI RCATGY 1 cut(s) 350
BstSLI GKGCMC 1 cut(s) 364
BstV2I GAAGAC 1 cut(s) 13
BsuRI GGCC 1 cut(s) 279
BtsCI GGATG 1 cut(s) 207
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 1 cut(s) 42
Cac8I GCNNGC 1 cut(s) 277
CciI TCATGA 1 cut(s) 207
CviAII CATG 3 cut(s) 208, 287, 347
CviJI RGCY 6 cut(s) 83, 89, 160, 177, 279, 285
CviKI_1 RGCY 6 cut(s) 83, 89, 160, 177, 279, 285
DpnI GATC 2 cut(s) 206, 268
DpnII GATC 2 cut(s) 204, 266
Eco24I GRGCYC 1 cut(s) 179
Eco57I CTGAAG 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 179
FaeI CATG 3 cut(s) 211, 290, 350
FaiI YATR 3 cut(s) 209, 288, 348
FalI AAGNNNNNCTT 2 cut(s) 274, 306
FatI CATG 3 cut(s) 207, 286, 346
FbaI TGATCA 1 cut(s) 204
FokI GGATG 1 cut(s) 214
FriOI GRGCYC 1 cut(s) 179
FspBI CTAG 1 cut(s) 90
HaeIII GGCC 1 cut(s) 279
Hin1II CATG 3 cut(s) 211, 290, 350
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HindIII AAGCTT 1 cut(s) 81
HinfI GANTC 2 cut(s) 136, 260
HpaI GTTAAC 1 cut(s) 111
HphI GGTGA 2 cut(s) 16, 331
Hpy166II GTNNAC 2 cut(s) 111, 362
Hpy188I TCNGA 1 cut(s) 248
Hpy188III TCNNGA 3 cut(s) 208, 264, 369
Hpy8I GTNNAC 2 cut(s) 111, 362
HpyAV CCTTC 1 cut(s) 236
HpyCH4III ACNGT 1 cut(s) 115
HpyCH4V TGCA 3 cut(s) 155, 275, 362
Hsp92II CATG 3 cut(s) 211, 290, 350
Ksp22I TGATCA 1 cut(s) 204
KspAI GTTAAC 1 cut(s) 111
Kzo9I GATC 2 cut(s) 204, 266
LmnI GCTCC 1 cut(s) 174
LpnPI CCDG 5 cut(s) 46, 174, 192, 198, 261
MaeI CTAG 1 cut(s) 90
MaeIII GTNAC 2 cut(s) 40, 92
MalI GATC 2 cut(s) 206, 268
MboI GATC 2 cut(s) 204, 266
MboII GAAGA 4 cut(s) 13, 80, 196, 235
MhlI GDGCHC 2 cut(s) 179, 364
MluCI AATT 3 cut(s) 236, 326, 355
MmeI TCCRAC 1 cut(s) 378
MnlI CCTC 6 cut(s) 58, 98, 157, 190, 242, 367
MseI TTAA 3 cut(s) 110, 141, 306
Mva1269I GAATGC 1 cut(s) 277
NdeII GATC 2 cut(s) 204, 266
NlaIII CATG 3 cut(s) 211, 290, 350
NlaIV GGNNCC 1 cut(s) 176
NmuCI GTSAC 1 cut(s) 40
NspI RCATGY 1 cut(s) 350
PagI TCATGA 1 cut(s) 207
PctI GAATGC 1 cut(s) 277
PfeI GAWTC 2 cut(s) 136, 260
PspN4I GGNNCC 1 cut(s) 176
SaqAI TTAA 3 cut(s) 110, 141, 306
Sau3AI GATC 2 cut(s) 204, 266
SduI GDGCHC 2 cut(s) 179, 364
SetI ASST 5 cut(s) 29, 85, 168, 228, 342
Sse9I AATT 3 cut(s) 236, 326, 355
SspMI CTAG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 115
TaqI TCGA 3 cut(s) 50, 263, 269
TasI AATT 3 cut(s) 236, 326, 355
TfiI GAWTC 2 cut(s) 136, 260
Tru1I TTAA 3 cut(s) 110, 141, 306
Tru9I TTAA 3 cut(s) 110, 141, 306
TscAI CASTG 1 cut(s) 49
TseFI GTSAC 1 cut(s) 40
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 3 cut(s) 81, 338, 368
TspRI CASTG 1 cut(s) 49
VneI GTGCAC 1 cut(s) 360
XapI RAATTY 1 cut(s) 326
XceI RCATGY 1 cut(s) 350
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.