Rmu_sc0001716.1_g000030

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001716.1
OG0001292
Physical Location & Seq
Forward (+)
111158 .. 113083
1926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001716.1_g000030.1.cds

Sequence Viewer

Length: 1926 bp
atggcgcctgctttatctctactagggccaccagctctccgccagcctaaacccacttttcaccatccctccacccctatctcaagattctcaacctaccaacaccttgcagaaggtcaatacctcgagtcttctctaccacccttggacccctgcaccaagtttctcttagaagccatcacacaccccaagagccgttccgattacaactacgacgagcaaccaaaggatctggctcactatcaaggagaggcgtggaagcatgaccccctcaccactctcaagctcatatgtagtcttcgaatgatggggaagctcaacgaagatcggttttacaaagtcatgttgtgggtgcacaataaccaccctctaaccatgtctttgaatgtcatggcttttgggggtttaggtatgttcaaggatcttttgggggttctgtatagggttttggaggattccatagaaaaaccaaataacgaatactcgcggtggaattatgattattcttgggaaaaagagacatcaacaaataaggaaggtattgataataattctagggctaaaactgccgttgagaggtaccaaaacgactcagattatcgaaacttccatgaccggatatcggagatgtttgcagaacttctgagatccgatctcaggtttttggagtcgggtgaggttgaaaaaatcagctttgcttccaaattttgtccttcagtcaattcttcatatgataaagcaacgctgatatgtgaaaatatagcaaagagaatgttcccgaagcattgttatgaagaatacaaagatgttgaggaagctcattatgcctacagggttcgtgagcgcctgcggaaacaggtactcattcctctgcgcaaagccttaaaattatcatcggtggagatgaagaatacgttggctttcgaaaacagcaaagcctttaatggtttgaaaatatacaaggagatttctaaccatggttatctaaagggaagcaaattactatttcaaaagtgcatgaagcttgtggagatgtttgggaatccaagcattggtgacgggctgaaacttcctaacgatattgtagcttccttgatgagcaaggaacatagtgatgaaattgtggagcgtcaatggcggaaactagtccaagagttgtcaaaccaaggcaagctgaggaattgtcttgccgtgtgtgaccttcccttgaacatgagagggacatacaaggagacagcttgtatttcactggggttattgatttcagagctgagtgaaaatccatggaaaggaaaggttttctcattcaatgaaactccaacacttcaaaagatcgaaggagacgatcttgaatccaagtgtgagttcatgagacacaacatagggtgtggagacaaagttgatttcttgaagatttacaaccaagtcttgcaaattgccattgaacaaaagctaagcaacgagcagttgcccaagaggatttttgtgttcacttatagggatttcgaggcggcttccaagaacaattgggcagaagattataaagaggcatggactaattacagaagaagagggtacgcaaatgtaccactggtggaggctaggactcaatacaaaaagaaagggtacaaaactctgccacacattttgttttggaatcttaagaattccactgctgagccagagataatttgtagccctgttaataacaaatgtggggggctgattataactgggttgtcgaacagtttgatcagtatgttcttagtgggagagtcggattcgagaacatatgcacctcatgtccaggcaccttatggtatcggttcgctgtcggtactgaaatttcttcccaaagctgaggatgtgatgaaatctgtatttttgagagatgaattattaaagaacctccttatattggattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

641

Amino Acids

73.85

Weight (kDa)

8.53

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1542, 1730
Acc16I TGCGCA 1 cut(s) 875
Acc65I GGTACC 1 cut(s) 579
AccB1I GGYRCC 3 cut(s) 4, 579, 1810
AccB7I CCANNNNNTGG 1 cut(s) 1052
AccII CGCG 1 cut(s) 487
AciI CCGC 5 cut(s) 40, 487, 850, 1138, 1511
AclWI GGATC 3 cut(s) 237, 429, 642
AcsI RAATTY 3 cut(s) 704, 1666, 1844
AcuI CTGAAG 1 cut(s) 699
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 6 cut(s) 581, 861, 1577, 1587, 1628, 1839
AfiI CCNNNNNNNGG 6 cut(s) 576, 622, 657, 1052, 1289, 1918
AflII CTTAAG 1 cut(s) 1661
AhlI ACTAGT 1 cut(s) 1144
AjnI CCWGG 1 cut(s) 1806
AjuI GAANNNNNNNTTGG 4 cut(s) 899, 931, 1349, 1381
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 5 cut(s) 512, 1226, 1335, 1366, 1386
Alw44I GTGCAC 1 cut(s) 353
AlwI GGATC 3 cut(s) 237, 429, 642
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 1 cut(s) 26
ApaLI GTGCAC 1 cut(s) 353
ApoI RAATTY 3 cut(s) 704, 1666, 1844
Asp718I GGTACC 1 cut(s) 579
AspLEI GCGC 3 cut(s) 7, 846, 876
AspS9I GGNCC 2 cut(s) 26, 148
AsuHPI GGTGA 4 cut(s) 53, 265, 686, 1067
AsuII TTCGAA 2 cut(s) 301, 924
AvaI CYCGRG 1 cut(s) 125
AvaII GGWCC 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 357
BanI GGYRCC 3 cut(s) 4, 579, 1810
BbsI GAAGAC 2 cut(s) 123, 290
Bbv12I GWGCWC 1 cut(s) 357
BbvCI CCTCAGC 2 cut(s) 1175, 1860
BccI CCATC 3 cut(s) 72, 185, 301
BceAI ACGGC 3 cut(s) 180, 554, 1175
BciT130I CCWGG 1 cut(s) 1808
BclI TGATCA 1 cut(s) 1752
BcoDI GTCTC 5 cut(s) 512, 1226, 1335, 1366, 1386
BcuI ACTAGT 1 cut(s) 1144
BfaI CTAG 4 cut(s) 23, 555, 1145, 1602
BfmI CTRYAG 1 cut(s) 829
BfoI RGCGCY 2 cut(s) 8, 847
BfrI CTTAAG 1 cut(s) 1661
BisI GCNGC 1 cut(s) 1512
BlpI GCTNAGC 2 cut(s) 1454, 1677
BlsI GCNGC 1 cut(s) 1513
Bme1390I CCNGG 1 cut(s) 1808
Bme18I GGWCC 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 2 cut(s) 26, 148
BmiI GGNNCC 4 cut(s) 6, 150, 581, 1812
BmrFI CCNGG 1 cut(s) 1808
BmrI ACTGGG 2 cut(s) 1259, 1743
BmuI ACTGGG 2 cut(s) 1259, 1743
BpiI GAAGAC 2 cut(s) 123, 290
Bpu10I CCTNAGC 2 cut(s) 1175, 1860
Bpu1102I GCTNAGC 2 cut(s) 1454, 1677
Bpu14I TTCGAA 2 cut(s) 301, 924
BpuEI CTTGAG 2 cut(s) 67, 266
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 144, 976, 1165, 1283
BsaWI WCCGGW 1 cut(s) 615
BsaXI ACNNNNNCTCC 6 cut(s) 21, 51, 53, 83, 1383, 1413
Bsc4I CCNNNNNNNGG 6 cut(s) 576, 622, 657, 1052, 1289, 1918
Bse1I ACTGG 3 cut(s) 1254, 1596, 1738
BseBI CCWGG 1 cut(s) 1808
BseDI CCNNGG 4 cut(s) 144, 976, 1165, 1283
BseGI GGATG 2 cut(s) 64, 1870
BseLI CCNNNNNNNGG 6 cut(s) 576, 622, 657, 1052, 1289, 1918
BseMII CTCAG 7 cut(s) 606, 635, 670, 1166, 1262, 1668, 1851
BseNI ACTGG 3 cut(s) 1254, 1596, 1738
BseSI GKGCMC 1 cut(s) 357
BsgI GTGCAG 1 cut(s) 139
Bsh1236I CGCG 1 cut(s) 487
BshFI GGCC 1 cut(s) 28
BshNI GGYRCC 3 cut(s) 4, 579, 1810
BsiHKAI GWGCWC 1 cut(s) 357
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 1 cut(s) 616
BslFI GGGAC 1 cut(s) 1234
BslI CCNNNNNNNGG 6 cut(s) 576, 622, 657, 1052, 1289, 1918
BsmAI GTCTC 5 cut(s) 512, 1226, 1335, 1366, 1386
BsmBI CGTCTC 1 cut(s) 1335
BsmFI GGGAC 1 cut(s) 1234
BsnI GGCC 1 cut(s) 28
BsoBI CYCGRG 1 cut(s) 125
Bsp119I TTCGAA 2 cut(s) 301, 924
Bsp1286I GDGCHC 1 cut(s) 357
Bsp143I GATC 8 cut(s) 229, 325, 421, 647, 652, 1332, 1345, 1752
Bsp1720I GCTNAGC 2 cut(s) 1454, 1677
Bsp19I CCATGG 2 cut(s) 976, 1283
BspACI CCGC 5 cut(s) 40, 487, 850, 1138, 1511
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 7 cut(s) 605, 636, 669, 1167, 1263, 1669, 1852
BspFNI CGCG 1 cut(s) 487
BspHI TCATGA 1 cut(s) 1368
BspLI GGNNCC 4 cut(s) 6, 150, 581, 1812
BspPI GGATC 3 cut(s) 237, 429, 642
BspT104I TTCGAA 2 cut(s) 301, 924
BspT107I GGYRCC 3 cut(s) 4, 579, 1810
BspTI CTTAAG 1 cut(s) 1661
BsrI ACTGG 3 cut(s) 1254, 1596, 1738
BssECI CCNNGG 4 cut(s) 144, 976, 1165, 1283
BssMI GATC 8 cut(s) 229, 325, 421, 647, 652, 1332, 1345, 1752
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 4 cut(s) 144, 976, 1165, 1283
Bst2UI CCWGG 1 cut(s) 1808
Bst4CI ACNGT 1 cut(s) 1748
Bst6I CTCTTC 1 cut(s) 1564
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 1661
BstBI TTCGAA 2 cut(s) 301, 924
BstC8I GCNNGC 4 cut(s) 9, 44, 848, 1172
BstDSI CCRYGG 2 cut(s) 976, 1283
BstF5I GGATG 2 cut(s) 64, 1870
BstFNI CGCG 1 cut(s) 487
BstH2I RGCGCY 2 cut(s) 8, 847
BstHHI GCGC 3 cut(s) 7, 846, 876
BstKTI GATC 8 cut(s) 232, 328, 424, 650, 655, 1335, 1348, 1755
BstMAI GTCTC 5 cut(s) 512, 1226, 1335, 1366, 1386
BstMBI GATC 8 cut(s) 229, 325, 421, 647, 652, 1332, 1345, 1752
BstMWI GCNNNNNNNGC 3 cut(s) 566, 824, 1135
BstNI CCWGG 1 cut(s) 1808
BstSCI CCNGG 1 cut(s) 1806
BstSFI CTRYAG 1 cut(s) 829
BstSLI GKGCMC 1 cut(s) 357
BstUI CGCG 1 cut(s) 487
BstV2I GAAGAC 2 cut(s) 123, 290
BstX2I RGATCY 3 cut(s) 229, 421, 647
BstYI RGATCY 3 cut(s) 229, 421, 647
BsuRI GGCC 1 cut(s) 28
BtgI CCRYGG 2 cut(s) 976, 1283
BtsCI GGATG 2 cut(s) 64, 1870
BtsI GCAGTG 1 cut(s) 1671
BtsIMutI CAGTG 3 cut(s) 1247, 1589, 1671
Cac8I GCNNGC 4 cut(s) 9, 44, 848, 1172
CciI TCATGA 1 cut(s) 1368
CfoI GCGC 3 cut(s) 7, 846, 876
Cfr13I GGNCC 2 cut(s) 26, 148
CseI GACGC 1 cut(s) 1118
Csp6I GTAC 6 cut(s) 580, 860, 1576, 1586, 1627, 1838
CspCI CAANNNNNGTGG 2 cut(s) 1629, 1664
CviQI GTAC 6 cut(s) 580, 860, 1576, 1586, 1627, 1838
DinI GGCGCC 1 cut(s) 6
DpnI GATC 8 cut(s) 231, 327, 423, 649, 654, 1334, 1347, 1754
DpnII GATC 8 cut(s) 229, 325, 421, 647, 652, 1332, 1345, 1752
Eam1104I CTCTTC 1 cut(s) 1564
EarI CTCTTC 1 cut(s) 1564
EciI GGCGGA 2 cut(s) 29, 1153
Eco130I CCWWGG 4 cut(s) 144, 976, 1165, 1283
Eco32I GATATC 1 cut(s) 621
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 699
Eco88I CYCGRG 1 cut(s) 125
EcoRI GAATTC 1 cut(s) 1666
EcoRII CCWGG 1 cut(s) 1806
EcoRV GATATC 1 cut(s) 621
EcoT14I CCWWGG 4 cut(s) 144, 976, 1165, 1283
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 4 cut(s) 144, 976, 1165, 1283
Esp3I CGTCTC 1 cut(s) 1335
FalI AAGNNNNNCTT 2 cut(s) 152, 184
FaqI GGGAC 1 cut(s) 1234
FauNDI CATATG 3 cut(s) 290, 730, 1792
FbaI TGATCA 1 cut(s) 1752
Fnu4HI GCNGC 1 cut(s) 1512
FokI GGATG 2 cut(s) 51, 1877
Fsp4HI GCNGC 1 cut(s) 1512
FspBI CTAG 4 cut(s) 23, 555, 1145, 1602
FspI TGCGCA 1 cut(s) 875
GlaI GCGC 3 cut(s) 6, 845, 875
GluI GCNGC 1 cut(s) 1512
HaeII RGCGCY 2 cut(s) 8, 847
HaeIII GGCC 1 cut(s) 28
HapII CCGG 1 cut(s) 616
HgaI GACGC 1 cut(s) 1118
HhaI GCGC 3 cut(s) 7, 846, 876
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 3 cut(s) 5, 844, 874
HinP1I GCGC 3 cut(s) 5, 844, 874
HindIII AAGCTT 1 cut(s) 1022
HpaII CCGG 1 cut(s) 616
HphI GGTGA 4 cut(s) 53, 265, 686, 1067
Hpy166II GTNNAC 2 cut(s) 355, 1491
Hpy188I TCNGA 7 cut(s) 202, 595, 625, 645, 652, 1267, 1780
Hpy188III TCNNGA 7 cut(s) 84, 778, 839, 1349, 1369, 1408, 1785
Hpy8I GTNNAC 2 cut(s) 355, 1491
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 5 cut(s) 107, 530, 723, 1211, 1331
HpyCH4III ACNGT 1 cut(s) 1748
HpyCH4IV ACGT 1 cut(s) 914
HpyCH4V TGCA 7 cut(s) 110, 156, 355, 635, 1017, 1432, 1796
HpyF10VI GCNNNNNNNGC 3 cut(s) 566, 824, 1135
HpySE526I ACGT 1 cut(s) 914
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 3 cut(s) 5, 844, 874
KasI GGCGCC 1 cut(s) 4
KpnI GGTACC 1 cut(s) 583
Ksp22I TGATCA 1 cut(s) 1752
Kzo9I GATC 8 cut(s) 229, 325, 421, 647, 652, 1332, 1345, 1752
LmnI GCTCC 1 cut(s) 1126
MaeI CTAG 4 cut(s) 23, 555, 1145, 1602
MaeII ACGT 1 cut(s) 914
MaeIII GTNAC 2 cut(s) 1055, 1196
MalI GATC 8 cut(s) 231, 327, 423, 649, 654, 1334, 1347, 1754
MboI GATC 8 cut(s) 229, 325, 421, 647, 652, 1332, 1345, 1752
MfeI CAATTG 1 cut(s) 1525
MflI RGATCY 3 cut(s) 229, 421, 647
MhlI GDGCHC 1 cut(s) 357
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 5 cut(s) 137, 584, 677, 1600, 1784
MmeI TCCRAC 2 cut(s) 1343, 1758
MseI TTAA 5 cut(s) 884, 942, 1662, 1704, 1901
MslI CAYNNNNRTG 2 cut(s) 789, 1113
MspCI CTTAAG 1 cut(s) 1661
MspI CCGG 1 cut(s) 616
MspR9I CCNGG 1 cut(s) 1808
MunI CAATTG 1 cut(s) 1525
MvaI CCWGG 1 cut(s) 1808
MvnI CGCG 1 cut(s) 487
MwoI GCNNNNNNNGC 3 cut(s) 566, 824, 1135
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 2 cut(s) 976, 1283
NdeI CATATG 3 cut(s) 290, 730, 1792
NdeII GATC 8 cut(s) 229, 325, 421, 647, 652, 1332, 1345, 1752
NlaIV GGNNCC 4 cut(s) 6, 150, 581, 1812
NmuCI GTSAC 2 cut(s) 1055, 1196
NsbI TGCGCA 1 cut(s) 875
NspV TTCGAA 2 cut(s) 301, 924
PaeR7I CTCGAG 1 cut(s) 125
PagI TCATGA 1 cut(s) 1368
PcsI WCGNNNNNNNCGW 1 cut(s) 1341
PfeI GAWTC 6 cut(s) 87, 455, 1042, 1352, 1657, 1781
PflMI CCANNNNNTGG 1 cut(s) 1052
PkrI GCNGC 1 cut(s) 1513
PleI GAGTC 5 cut(s) 136, 584, 676, 1600, 1783
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 5 cut(s) 136, 584, 676, 1600, 1783
PsiI TTATAA 2 cut(s) 1542, 1730
Psp6I CCWGG 1 cut(s) 1806
PspGI CCWGG 1 cut(s) 1806
PspN4I GGNNCC 4 cut(s) 6, 150, 581, 1812
PspPI GGNCC 2 cut(s) 26, 148
PspXI VCTCGAGB 1 cut(s) 125
PsuI RGATCY 3 cut(s) 229, 421, 647
RsaI GTAC 6 cut(s) 581, 861, 1577, 1587, 1628, 1839
RsaNI GTAC 6 cut(s) 580, 860, 1576, 1586, 1627, 1838
RseI CAYNNNNRTG 2 cut(s) 789, 1113
SaqAI TTAA 5 cut(s) 884, 942, 1662, 1704, 1901
SatI GCNGC 1 cut(s) 1512
Sau3AI GATC 8 cut(s) 229, 325, 421, 647, 652, 1332, 1345, 1752
Sau96I GGNCC 2 cut(s) 26, 148
SchI GAGTC 5 cut(s) 137, 584, 677, 1600, 1784
ScrFI CCNGG 1 cut(s) 1808
SduI GDGCHC 1 cut(s) 357
SfcI CTRYAG 1 cut(s) 829
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 125
SfuI TTCGAA 2 cut(s) 301, 924
SinI GGWCC 1 cut(s) 148
SlaI CTCGAG 1 cut(s) 125
SmiMI CAYNNNNRTG 2 cut(s) 789, 1113
SmlI CTYRAG 4 cut(s) 82, 125, 281, 1661
SmoI CTYRAG 4 cut(s) 82, 125, 281, 1661
SpeI ACTAGT 1 cut(s) 1144
SsiI CCGC 5 cut(s) 40, 487, 850, 1138, 1511
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 4 cut(s) 23, 555, 1145, 1602
StyD4I CCNGG 1 cut(s) 1806
StyI CCWWGG 4 cut(s) 144, 976, 1165, 1283
TaaI ACNGT 1 cut(s) 1748
TaiI ACGT 1 cut(s) 917
TaqI TCGA 8 cut(s) 126, 301, 601, 924, 1335, 1506, 1742, 1784
TauI GCSGC 1 cut(s) 1514
TfiI GAWTC 6 cut(s) 87, 455, 1042, 1352, 1657, 1781
Tru1I TTAA 5 cut(s) 884, 942, 1662, 1704, 1901
Tru9I TTAA 5 cut(s) 884, 942, 1662, 1704, 1901
TscAI CASTG 3 cut(s) 1254, 1596, 1678
TseFI GTSAC 2 cut(s) 1055, 1196
Tsp45I GTSAC 2 cut(s) 1055, 1196
TspDTI ATGAA 9 cut(s) 717, 807, 920, 1034, 1131, 1326, 1357, 1886, 1908
TspRI CASTG 3 cut(s) 1254, 1596, 1678
Van91I CCANNNNNTGG 1 cut(s) 1052
Vha464I CTTAAG 1 cut(s) 1661
VneI GTGCAC 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 148
XapI RAATTY 3 cut(s) 704, 1666, 1844
XcmI CCANNNNNNNNNTGG 1 cut(s) 1814
XhoI CTCGAG 1 cut(s) 125
XspI CTAG 4 cut(s) 23, 555, 1145, 1602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.