Rorug05G0549000

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001292
Physical Location & Seq
Forward (+)
73778022 .. 73778996
975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0549000.1

Sequence Viewer

Length: 975 bp
ATGGTAGGGCGTTTGCTGGGTCCCAAAACTATGTTTCCCGGTTTTAAGGGCACGATTGCTACCATCTGGAGGATGCGATCAGGGTTAACAATTCAAGATGCAGGAGATTGGTTTGTCTTCCAGTTTGATCGTGAAGCTGATCGCATTAAGATATTACATGGAGGACCGTGGTTCTACCGGAACACCATGTTGGTGTTGGGTGTGTATGGTGGTGCAGGGCCAGTGGCGGAAGTCCCGCTCAATCTGTTAGAGACCTGGGTTGTGGTCAAGGGCCTTCCACTGGTTCTCCGTAACAAGACTGCGCTAGGATTAGTTGGGTCATCCCTTGGTCAAGTAATCTGTTTTGATCAGTCAGCCCTTAAACGGAAGGAGGAGGAGCAAAGCATTCATCTCGTCCTCGATGTGCATTCTAGGATTAGGGTGTGGATGGTGTTTGAACTGACACTGGTGTATGAGAAGATAAAGGGATTTTACAGGAACTGTGGGCTTTTTCTGCATGATGCTGCAGGTTGTGACTCATTGCTGGTGAAGGAAAGAGAAGAGATTCTTGCGCAAGCATCGGCGGTGGCTATGGCTGGGCTCTCTTTAATCTCAGATGGGCAAGTCGCTAGGGATGACAGTGAGGGAGAAATTCCGAGTGGTAGTAAGGGAATGGTAAGAATGGCGCTGACTTTGGTGGGTGATGGGGTCATACCGGATATGGTGGAAGCCTATGCATCAATGCCTTCCAACAATATGGGAGCCTCAAGCCAAGTTGAGAAAGCTTTTGCTTGTTGTAATATGGCTGACTTTCTAAAGAATTGGGATGTGCAGGCTAACAAGAGTACGGGGTTTAGGGCTGTCCCTGGAGAGATGCCATTGTTGACGGCTACTCAGCCTTTGCGTCCTGACTTGGATACACATTTACGCAAGCGTACGTATCATTGTCAAGATAGGGAAATATTATGCCCAAAATTATCGTACCACGGGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

324

Amino Acids

35.78

Weight (kDa)

6.66

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 2 - 66 4.1e-08 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 552
Acc36I ACCTGC 1 cut(s) 497
AccBSI CCGCTC 1 cut(s) 238
AciI CCGC 3 cut(s) 227, 236, 563
AcsI RAATTY 1 cut(s) 630
AfaI GTAC 3 cut(s) 826, 916, 962
AfiI CCNNNNNNNGG 3 cut(s) 69, 280, 363
AgsI TTSAA 2 cut(s) 95, 437
AjnI CCWGG 2 cut(s) 254, 844
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AluBI AGCT 2 cut(s) 137, 764
AluI AGCT 2 cut(s) 137, 764
Alw26I GTCTC 1 cut(s) 245
AlwNI CAGNNNCTG 1 cut(s) 480
AoxI GGCC 2 cut(s) 218, 271
ApeKI GCWGC 1 cut(s) 503
ApoI RAATTY 1 cut(s) 630
Asp700I GAANNNNTTC 1 cut(s) 543
AspLEI GCGC 3 cut(s) 304, 553, 667
AspS9I GGNCC 4 cut(s) 20, 164, 218, 271
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 2 cut(s) 538, 692
AvaII GGWCC 2 cut(s) 20, 164
BaeGI GKGCMC 1 cut(s) 53
BanII GRGCYC 1 cut(s) 582
BbsI GAAGAC 1 cut(s) 109
BbvI GCAGC 1 cut(s) 490
BccI CCATC 4 cut(s) 71, 421, 590, 677
BceAI ACGGC 1 cut(s) 882
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BciT130I CCWGG 2 cut(s) 256, 846
BciVI GTATCC 1 cut(s) 889
BclI TGATCA 1 cut(s) 346
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 3 cut(s) 305, 411, 609
BfmI CTRYAG 1 cut(s) 504
BfoI RGCGCY 1 cut(s) 668
BfuAI ACCTGC 1 cut(s) 497
BfuI GTATCC 1 cut(s) 889
BisI GCNGC 1 cut(s) 504
BlsI GCNGC 1 cut(s) 505
Bme1390I CCNGG 3 cut(s) 39, 256, 846
Bme18I GGWCC 2 cut(s) 20, 164
BmgT120I GGNCC 4 cut(s) 20, 164, 218, 271
BmiI GGNNCC 3 cut(s) 21, 22, 742
BmrFI CCNGG 3 cut(s) 39, 256, 846
BmsI GCATC 6 cut(s) 63, 88, 490, 566, 725, 843
BpiI GAAGAC 1 cut(s) 109
BpmI CTGGAG 2 cut(s) 88, 867
BpuEI CTTGAG 1 cut(s) 730
BpuMI CCSGG 1 cut(s) 39
BsaAI YACGTR 1 cut(s) 918
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 5 cut(s) 167, 255, 325, 844, 964
BsaWI WCCGGW 2 cut(s) 177, 694
BsaXI ACNNNNNCTCC 2 cut(s) 270, 300
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 280, 363
Bse1I ACTGG 4 cut(s) 121, 221, 285, 450
Bse3DI GCAATG 1 cut(s) 518
BseBI CCWGG 2 cut(s) 256, 846
BseDI CCNNGG 5 cut(s) 167, 255, 325, 844, 964
BseGI GGATG 5 cut(s) 78, 320, 432, 619, 811
BseLI CCNNNNNNNGG 3 cut(s) 69, 280, 363
BseMI GCAATG 1 cut(s) 518
BseMII CTCAG 2 cut(s) 606, 887
BseNI ACTGG 4 cut(s) 121, 221, 285, 450
BseRI GAGGAG 2 cut(s) 386, 389
BseSI GKGCMC 1 cut(s) 53
BseXI GCAGC 1 cut(s) 490
BseYI CCCAGC 2 cut(s) 16, 575
BsgI GTGCAG 2 cut(s) 234, 830
BshFI GGCC 2 cut(s) 220, 273
BsiSI CCGG 3 cut(s) 39, 178, 695
BsiWI CGTACG 1 cut(s) 914
BslFI GGGAC 3 cut(s) 6, 218, 827
BslI CCNNNNNNNGG 3 cut(s) 69, 280, 363
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 3 cut(s) 6, 218, 827
BsmI GAATGC 2 cut(s) 384, 406
BsnI GGCC 2 cut(s) 220, 273
Bso31I GGTCTC 1 cut(s) 245
Bsp1286I GDGCHC 2 cut(s) 53, 582
Bsp143I GATC 4 cut(s) 77, 127, 139, 346
BspACI CCGC 3 cut(s) 227, 236, 563
BspANI GGCC 2 cut(s) 220, 273
BspCNI CTCAG 2 cut(s) 605, 886
BspLI GGNNCC 3 cut(s) 21, 22, 742
BspMAI CTGCAG 1 cut(s) 508
BspMI ACCTGC 1 cut(s) 497
BspTNI GGTCTC 1 cut(s) 245
BsrBI CCGCTC 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 518
BsrI ACTGG 4 cut(s) 121, 221, 285, 450
BssECI CCNNGG 5 cut(s) 167, 255, 325, 844, 964
BssMI GATC 4 cut(s) 77, 127, 139, 346
BssT1I CCWWGG 1 cut(s) 325
Bst2UI CCWGG 2 cut(s) 256, 846
Bst4CI ACNGT 3 cut(s) 168, 482, 620
Bst6I CTCTTC 1 cut(s) 534
BstBAI YACGTR 1 cut(s) 918
BstC8I GCNNGC 3 cut(s) 555, 813, 911
BstDEI CTNAG 2 cut(s) 592, 873
BstDSI CCRYGG 2 cut(s) 167, 964
BstF5I GGATG 5 cut(s) 78, 320, 432, 619, 811
BstH2I RGCGCY 1 cut(s) 668
BstHHI GCGC 3 cut(s) 304, 553, 667
BstKTI GATC 4 cut(s) 80, 130, 142, 349
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 4 cut(s) 77, 127, 139, 346
BstMWI GCNNNNNNNGC 1 cut(s) 493
BstNI CCWGG 2 cut(s) 256, 846
BstSCI CCNGG 3 cut(s) 37, 254, 844
BstSFI CTRYAG 1 cut(s) 504
BstSLI GKGCMC 1 cut(s) 53
BstSNI TACGTA 1 cut(s) 918
BstV1I GCAGC 1 cut(s) 490
BstV2I GAAGAC 1 cut(s) 109
BstXI CCANNNNNNTGG 1 cut(s) 736
BsuI GTATCC 1 cut(s) 889
BsuRI GGCC 2 cut(s) 220, 273
BtgI CCRYGG 2 cut(s) 167, 964
BtsCI GGATG 5 cut(s) 78, 320, 432, 619, 811
BtsIMutI CAGTG 4 cut(s) 228, 278, 443, 625
BveI ACCTGC 1 cut(s) 497
Cac8I GCNNGC 3 cut(s) 555, 813, 911
CaiI CAGNNNCTG 1 cut(s) 480
CfoI GCGC 3 cut(s) 304, 553, 667
Cfr13I GGNCC 4 cut(s) 20, 164, 218, 271
CseI GACGC 1 cut(s) 872
Csp6I GTAC 3 cut(s) 825, 915, 961
CviAII CATG 3 cut(s) 158, 187, 497
CviQI GTAC 3 cut(s) 825, 915, 961
DdeI CTNAG 2 cut(s) 592, 873
DpnI GATC 4 cut(s) 79, 129, 141, 348
DpnII GATC 4 cut(s) 77, 127, 139, 346
Eam1104I CTCTTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 534
EciI GGCGGA 1 cut(s) 242
Eco105I TACGTA 1 cut(s) 918
Eco130I CCWWGG 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 582
Eco31I GGTCTC 1 cut(s) 245
Eco47I GGWCC 2 cut(s) 20, 164
EcoO109I RGGNCCY 2 cut(s) 20, 271
EcoRII CCWGG 2 cut(s) 254, 844
EcoT14I CCWWGG 1 cut(s) 325
EcoT22I ATGCAT 1 cut(s) 718
EcoT38I GRGCYC 1 cut(s) 582
ErhI CCWWGG 1 cut(s) 325
FaeI CATG 3 cut(s) 161, 190, 500
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FaqI GGGAC 3 cut(s) 6, 218, 827
FatI CATG 3 cut(s) 157, 186, 496
FauI CCCGC 1 cut(s) 243
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 1 cut(s) 504
FokI GGATG 5 cut(s) 85, 307, 439, 626, 818
FriOI GRGCYC 1 cut(s) 582
Fsp4HI GCNGC 1 cut(s) 504
FspBI CTAG 3 cut(s) 305, 411, 609
FspI TGCGCA 1 cut(s) 552
GlaI GCGC 3 cut(s) 303, 552, 666
GluI GCNGC 1 cut(s) 504
GsaI CCCAGC 2 cut(s) 20, 579
GsuI CTGGAG 2 cut(s) 88, 867
HaeII RGCGCY 1 cut(s) 668
HaeIII GGCC 2 cut(s) 220, 273
HapII CCGG 3 cut(s) 39, 178, 695
HgaI GACGC 1 cut(s) 872
HhaI GCGC 3 cut(s) 304, 553, 667
Hin1II CATG 3 cut(s) 161, 190, 500
Hin6I GCGC 3 cut(s) 302, 551, 665
HinP1I GCGC 3 cut(s) 302, 551, 665
HincII GTYRAC 2 cut(s) 87, 864
HindII GTYRAC 2 cut(s) 87, 864
HindIII AAGCTT 1 cut(s) 762
HinfI GANTC 2 cut(s) 515, 544
HpaI GTTAAC 1 cut(s) 87
HpaII CCGG 3 cut(s) 39, 178, 695
HphI GGTGA 2 cut(s) 538, 692
Hpy166II GTNNAC 2 cut(s) 87, 864
Hpy188I TCNGA 2 cut(s) 595, 636
Hpy188III TCNNGA 5 cut(s) 67, 95, 131, 887, 929
Hpy8I GTNNAC 2 cut(s) 87, 864
HpyAV CCTTC 4 cut(s) 284, 361, 523, 735
HpyCH4III ACNGT 3 cut(s) 168, 482, 620
HpyCH4IV ACGT 1 cut(s) 917
HpyCH4V TGCA 7 cut(s) 101, 215, 406, 496, 506, 716, 811
HpyF10VI GCNNNNNNNGC 1 cut(s) 493
HpyF3I CTNAG 2 cut(s) 592, 873
HpySE526I ACGT 1 cut(s) 917
Hsp92II CATG 3 cut(s) 161, 190, 500
HspAI GCGC 3 cut(s) 302, 551, 665
KflI GGGWCCC 1 cut(s) 20
Ksp22I TGATCA 1 cut(s) 346
KspAI GTTAAC 1 cut(s) 87
Kzo9I GATC 4 cut(s) 77, 127, 139, 346
LmnI GCTCC 2 cut(s) 376, 740
Lsp1109I GCAGC 1 cut(s) 490
LweI GCATC 6 cut(s) 63, 88, 490, 566, 725, 843
MaeI CTAG 3 cut(s) 305, 411, 609
MaeII ACGT 1 cut(s) 917
MaeIII GTNAC 2 cut(s) 290, 512
MalI GATC 4 cut(s) 79, 129, 141, 348
MbiI CCGCTC 1 cut(s) 238
MboI GATC 4 cut(s) 77, 127, 139, 346
MboII GAAGA 3 cut(s) 109, 469, 551
MhlI GDGCHC 2 cut(s) 53, 582
MluCI AATT 4 cut(s) 90, 630, 799, 953
MlyI GAGTC 1 cut(s) 509
MmeI TCCRAC 1 cut(s) 753
MnlI CCTC 7 cut(s) 63, 155, 364, 367, 407, 616, 754
Mph1103I ATGCAT 1 cut(s) 718
MroXI GAANNNNTTC 1 cut(s) 543
MseI TTAA 5 cut(s) 45, 86, 147, 360, 587
MslI CAYNNNNRTG 1 cut(s) 191
MspI CCGG 3 cut(s) 39, 178, 695
MspR9I CCNGG 3 cut(s) 39, 256, 846
Mva1269I GAATGC 2 cut(s) 384, 406
MvaI CCWGG 2 cut(s) 256, 846
MwoI GCNNNNNNNGC 1 cut(s) 493
NciI CCSGG 1 cut(s) 39
NdeII GATC 4 cut(s) 77, 127, 139, 346
NlaIII CATG 3 cut(s) 161, 190, 500
NlaIV GGNNCC 3 cut(s) 21, 22, 742
NmuCI GTSAC 1 cut(s) 512
NsbI TGCGCA 1 cut(s) 552
NsiI ATGCAT 1 cut(s) 718
PctI GAATGC 2 cut(s) 384, 406
PdmI GAANNNNTTC 1 cut(s) 543
PfeI GAWTC 1 cut(s) 544
Pfl23II CGTACG 1 cut(s) 914
PkrI GCNGC 1 cut(s) 505
PleI GAGTC 1 cut(s) 509
PpsI GAGTC 1 cut(s) 509
Ppu21I YACGTR 1 cut(s) 918
PpuMI RGGWCCY 1 cut(s) 20
Psp5II RGGWCCY 1 cut(s) 20
Psp6I CCWGG 2 cut(s) 254, 844
PspFI CCCAGC 2 cut(s) 16, 575
PspGI CCWGG 2 cut(s) 254, 844
PspLI CGTACG 1 cut(s) 914
PspN4I GGNNCC 3 cut(s) 21, 22, 742
PspPI GGNCC 4 cut(s) 20, 164, 218, 271
PspPPI RGGWCCY 1 cut(s) 20
PstI CTGCAG 1 cut(s) 508
PstNI CAGNNNCTG 1 cut(s) 480
RsaI GTAC 3 cut(s) 826, 916, 962
RsaNI GTAC 3 cut(s) 825, 915, 961
RseI CAYNNNNRTG 1 cut(s) 191
SaqAI TTAA 5 cut(s) 45, 86, 147, 360, 587
SatI GCNGC 1 cut(s) 504
Sau3AI GATC 4 cut(s) 77, 127, 139, 346
Sau96I GGNCC 4 cut(s) 20, 164, 218, 271
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 3 cut(s) 39, 256, 846
SduI GDGCHC 2 cut(s) 53, 582
SetI ASST 5 cut(s) 139, 257, 511, 766, 920
SfaNI GCATC 6 cut(s) 63, 88, 490, 566, 725, 843
SfcI CTRYAG 1 cut(s) 504
SinI GGWCC 2 cut(s) 20, 164
SmiMI CAYNNNNRTG 1 cut(s) 191
SmlI CTYRAG 1 cut(s) 745
SmoI CTYRAG 1 cut(s) 745
SnaBI TACGTA 1 cut(s) 918
Sse9I AATT 4 cut(s) 90, 630, 799, 953
SsiI CCGC 3 cut(s) 227, 236, 563
SspI AATATT 1 cut(s) 942
SspMI CTAG 3 cut(s) 305, 411, 609
StyD4I CCNGG 3 cut(s) 37, 254, 844
StyI CCWWGG 1 cut(s) 325
TaaI ACNGT 3 cut(s) 168, 482, 620
TaiI ACGT 1 cut(s) 920
TaqI TCGA 1 cut(s) 399
TasI AATT 4 cut(s) 90, 630, 799, 953
TfiI GAWTC 1 cut(s) 544
Tru1I TTAA 5 cut(s) 45, 86, 147, 360, 587
Tru9I TTAA 5 cut(s) 45, 86, 147, 360, 587
TscAI CASTG 4 cut(s) 228, 285, 450, 625
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 1 cut(s) 503
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 1 cut(s) 377
TspGWI ACGGA 2 cut(s) 278, 379
TspRI CASTG 4 cut(s) 228, 285, 450, 625
VpaK11BI GGWCC 2 cut(s) 20, 164
XapI RAATTY 1 cut(s) 630
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XmnI GAANNNNTTC 1 cut(s) 543
XspI CTAG 3 cut(s) 305, 411, 609
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.