MD05G1095800.v1.1

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001292
Physical Location & Seq
Reverse (-)
20192996 .. 20194327
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1095800.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGGCTCCTCCAATTTGTCTTTTGGGCCCACCCGAGCTCCGCCTTACCCACCCCACCGCAACCCATGAAGAAAACCCTCCTCTGGGTAAATACCAGTCATCTTCCTCAACCCCAACCTTGAATCCATGCCTTGAATTCTTCTTTAAAGCCATCACCACATCCTCAGATCCTAAACGCCCACAGAACCCTGCCCCCAAATACGGTTACAAGTATGAGCGACAGAAGGATCTTACAGAATGTGAAGAAGAAGCATGGAATCAAGACCCTCTCATCACTCTGGGACTCATATTTTGCCTCAGAATGGTGTGGAGGCCCAACATAGAAGCCTTTTACAAGTCCTTCTTGTGGGTCCACAAAAACCACCCTTTAGCCCTAGCTCTAAATCTCAACGTTTTTGGGGATTTGGGTTGGTTCAAAGATTTGTTGGAGATTCTCTCTAGGGTTTTGCAGGACTCCATAGTAAAGAATAGGGAGGAGGAGAAGCGTATGAATGGTTGTTGGAACAGGAGTTATTGGTCCTTTTGCAATGAAAGCTATGAGGAGGAGGAAGAGAAGGAAGAGAAGGAGGAGATCAATACAATCACAGATGTTCAAATTGCCAGGGCTAAAGCGGCTGTTGATAGGTACCGAGATGACCCGGAGTATAGGTTCTTGCACGATCGTGTATCTGATGTGTTTGTTGAAATGTTGGCATCAGATTTAAGGTTTTTGAACTCTGATGAGATTGAAAAAATCAGCTATGCTTCCAAATTTTGCCCGTCAATTGATTCGTCTTACGACAGAACAACTCTAATATGTGAAAACATAGCGAAGAGAATGTTTCCACGGGACGTTTATGAAGAGTACATAGATCTTGAAGAAGCGCATTACGCTTACAGGGTTCGCGATCGGTTGAGGAAACAAGTACTTATCCCCCTGCGCAAGGCATTGTCAGTGGATTGCTCACTGGCTGCTAAACAGAACAGGAAGTCATTGTCATTGTTCAAAAACCGCAAGGCGCTGATTGCTTTAGAAGACTACAGGAATATCGCTGGTTGTCATGATGGTGGAGGCAACAACGAAAGCAAATTGAAATTCAAGAGTCGCATGAATATTGTGCAAAAGCTTGTTCCAAAGAAAGCAATTATCGATATGTGTTTTGGTGATGAGGTAAAGCTTCCTCACCACGTGCTGGTCTCATTGGAGAAGGAACAAGGTGGGGTTACTGAAACCGCAGAGGTTCAGTGGCAGAGATTGGTCAAAGAGTTGTCTAGCAAAGGGAAACTCAGAAATTGTGTTGCAGTGTGTGATGTCCCGGAGAGTATGAGAGGGGCGTTGAAGGAGAAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

444

Amino Acids

51.3

Weight (kDa)

6.55

Isoelectric Point (pI)

51.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 39 - 422 6.9e-65 Domain of unknown function (DUF2828)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 920
Acc65I GGTACC 1 cut(s) 624
AccB1I GGYRCC 1 cut(s) 624
AccB7I CCANNNNNTGG 1 cut(s) 1171
AccII CGCG 1 cut(s) 885
AciI CCGC 5 cut(s) 40, 57, 611, 991, 1212
AclI AACGTT 1 cut(s) 390
AclWI GGATC 2 cut(s) 161, 234
AcsI RAATTY 3 cut(s) 134, 749, 1073
AcvI CACGTG 1 cut(s) 1168
AdeI CACNNNGTG 1 cut(s) 1168
AfaI GTAC 3 cut(s) 626, 845, 906
AfiI CCNNNNNNNGG 7 cut(s) 82, 83, 200, 301, 345, 922, 1171
AjnI CCWGG 1 cut(s) 599
AleI CACNNNNGTG 1 cut(s) 660
AloI GAACNNNNNNTCC 2 cut(s) 632, 664
AluBI AGCT 6 cut(s) 37, 377, 534, 738, 1105, 1156
AluI AGCT 6 cut(s) 37, 377, 534, 738, 1105, 1156
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 1180
AlwI GGATC 2 cut(s) 161, 234
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 2 cut(s) 25, 311
ApaI GGGCCC 1 cut(s) 29
ApeKI GCWGC 1 cut(s) 950
ApoI RAATTY 3 cut(s) 134, 749, 1073
ArsI GACNNNNNNTTYG 2 cut(s) 273, 305
Asp718I GGTACC 1 cut(s) 624
AspLEI GCGC 3 cut(s) 865, 921, 1000
AspS9I GGNCC 5 cut(s) 25, 26, 312, 349, 516
AsuC2I CCSGG 2 cut(s) 638, 1295
AsuHPI GGTGA 3 cut(s) 145, 1154, 1154
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 2 cut(s) 349, 516
BaeGI GKGCMC 1 cut(s) 29
BanI GGYRCC 1 cut(s) 624
BanII GRGCYC 2 cut(s) 29, 39
BbrPI CACGTG 1 cut(s) 1168
BbsI GAAGAC 1 cut(s) 1020
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 1 cut(s) 937
BccI CCATC 2 cut(s) 158, 1037
BciT130I CCWGG 1 cut(s) 601
BcnI CCSGG 2 cut(s) 638, 1295
BcoDI GTCTC 1 cut(s) 1180
BfaI CTAG 3 cut(s) 374, 438, 1251
BfmI CTRYAG 1 cut(s) 1018
BfoI RGCGCY 1 cut(s) 1001
BglII AGATCT 1 cut(s) 850
BisI GCNGC 2 cut(s) 612, 951
BlsI GCNGC 2 cut(s) 613, 952
BmcAI AGTACT 1 cut(s) 906
Bme1390I CCNGG 3 cut(s) 601, 638, 1295
Bme18I GGWCC 2 cut(s) 349, 516
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 5 cut(s) 25, 26, 312, 349, 516
BmiI GGNNCC 4 cut(s) 6, 27, 350, 626
BmrFI CCNGG 3 cut(s) 601, 638, 1295
BmsI GCATC 1 cut(s) 701
BpiI GAAGAC 1 cut(s) 1020
BplI GAGNNNNNCTC 2 cut(s) 419, 451
BpuMI CCSGG 2 cut(s) 638, 1295
Bsa29I ATCGAT 1 cut(s) 1128
BsaAI YACGTR 1 cut(s) 1168
BsaI GGTCTC 1 cut(s) 1180
BsaJI CCNNGG 2 cut(s) 600, 824
BsaXI ACNNNNNCTCC 4 cut(s) 21, 51, 632, 662
Bsc4I CCNNNNNNNGG 7 cut(s) 82, 83, 200, 301, 345, 922, 1171
Bse1I ACTGG 2 cut(s) 94, 951
Bse3DI GCAATG 1 cut(s) 532
BseBI CCWGG 1 cut(s) 601
BseCI ATCGAT 1 cut(s) 1128
BseDI CCNNGG 2 cut(s) 600, 824
BseGI GGATG 1 cut(s) 158
BseLI CCNNNNNNNGG 7 cut(s) 82, 83, 200, 301, 345, 922, 1171
BseMI GCAATG 1 cut(s) 532
BseMII CTCAG 3 cut(s) 177, 310, 1279
BseNI ACTGG 2 cut(s) 94, 951
BseRI GAGGAG 6 cut(s) 69, 488, 491, 554, 557, 581
BseSI GKGCMC 1 cut(s) 29
BseXI GCAGC 1 cut(s) 937
Bsh1236I CGCG 1 cut(s) 885
Bsh1285I CGRYCG 2 cut(s) 661, 889
BshFI GGCC 2 cut(s) 27, 313
BshNI GGYRCC 1 cut(s) 624
BshVI ATCGAT 1 cut(s) 1128
BsiEI CGRYCG 2 cut(s) 661, 889
BsiHKAI GWGCWC 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 2 cut(s) 638, 1295
BslFI GGGAC 3 cut(s) 294, 842, 1277
BslI CCNNNNNNNGG 7 cut(s) 82, 83, 200, 301, 345, 922, 1171
BsmAI GTCTC 1 cut(s) 1180
BsmFI GGGAC 3 cut(s) 294, 842, 1277
BsnI GGCC 2 cut(s) 27, 313
Bso31I GGTCTC 1 cut(s) 1180
BsoBI CYCGRG 1 cut(s) 32
Bsp120I GGGCCC 1 cut(s) 25
Bsp1286I GDGCHC 2 cut(s) 29, 39
Bsp143I GATC 6 cut(s) 166, 226, 570, 658, 850, 886
Bsp68I TCGCGA 1 cut(s) 885
BspACI CCGC 5 cut(s) 40, 57, 611, 991, 1212
BspANI GGCC 2 cut(s) 27, 313
BspCNI CTCAG 3 cut(s) 176, 309, 1278
BspDI ATCGAT 1 cut(s) 1128
BspFNI CGCG 1 cut(s) 885
BspHI TCATGA 1 cut(s) 1039
BspLI GGNNCC 4 cut(s) 6, 27, 350, 626
BspPI GGATC 2 cut(s) 161, 234
BspT107I GGYRCC 1 cut(s) 624
BspTNI GGTCTC 1 cut(s) 1180
BsrDI GCAATG 1 cut(s) 532
BsrI ACTGG 2 cut(s) 94, 951
BssECI CCNNGG 2 cut(s) 600, 824
BssMI GATC 6 cut(s) 166, 226, 570, 658, 850, 886
Bst2UI CCWGG 1 cut(s) 601
Bst4CI ACNGT 1 cut(s) 203
Bst6I CTCTTC 4 cut(s) 543, 552, 806, 834
BstBAI YACGTR 1 cut(s) 1168
BstDEI CTNAG 3 cut(s) 163, 296, 1265
BstDSI CCRYGG 1 cut(s) 824
BstENI CCTNNNNNAGG 1 cut(s) 920
BstF5I GGATG 1 cut(s) 158
BstFNI CGCG 1 cut(s) 885
BstH2I RGCGCY 1 cut(s) 1001
BstHHI GCGC 3 cut(s) 865, 921, 1000
BstKTI GATC 6 cut(s) 169, 229, 573, 661, 853, 889
BstMAI GTCTC 1 cut(s) 1180
BstMBI GATC 6 cut(s) 166, 226, 570, 658, 850, 886
BstMCI CGRYCG 2 cut(s) 661, 889
BstMWI GCNNNNNNNGC 4 cut(s) 531, 611, 869, 1004
BstNI CCWGG 1 cut(s) 601
BstSCI CCNGG 3 cut(s) 599, 636, 1293
BstSFI CTRYAG 1 cut(s) 1018
BstSLI GKGCMC 1 cut(s) 29
BstUI CGCG 1 cut(s) 885
BstV1I GCAGC 1 cut(s) 937
BstV2I GAAGAC 1 cut(s) 1020
BstX2I RGATCY 3 cut(s) 166, 226, 850
BstYI RGATCY 3 cut(s) 166, 226, 850
Bsu15I ATCGAT 1 cut(s) 1128
BsuRI GGCC 2 cut(s) 27, 313
BsuTUI ATCGAT 1 cut(s) 1128
BtgI CCRYGG 1 cut(s) 824
BtsCI GGATG 1 cut(s) 158
BtsI GCAGTG 1 cut(s) 1287
BtsIMutI CAGTG 4 cut(s) 939, 944, 1229, 1287
BtuMI TCGCGA 1 cut(s) 885
CciI TCATGA 1 cut(s) 1039
CfoI GCGC 3 cut(s) 865, 921, 1000
Cfr13I GGNCC 5 cut(s) 25, 26, 312, 349, 516
ClaI ATCGAT 1 cut(s) 1128
Csp6I GTAC 3 cut(s) 625, 844, 905
CviAII CATG 5 cut(s) 65, 126, 252, 1040, 1087
CviQI GTAC 3 cut(s) 625, 844, 905
DdeI CTNAG 3 cut(s) 163, 296, 1265
DpnI GATC 6 cut(s) 168, 228, 572, 660, 852, 888
DpnII GATC 6 cut(s) 166, 226, 570, 658, 850, 886
DraI TTTAAA 1 cut(s) 145
DraIII CACNNNGTG 1 cut(s) 1168
Eam1104I CTCTTC 4 cut(s) 543, 552, 806, 834
EarI CTCTTC 4 cut(s) 543, 552, 806, 834
EciI GGCGGA 1 cut(s) 29
Ecl136II GAGCTC 1 cut(s) 37
Eco24I GRGCYC 2 cut(s) 29, 39
Eco31I GGTCTC 1 cut(s) 1180
Eco47I GGWCC 2 cut(s) 349, 516
Eco53kI GAGCTC 1 cut(s) 37
Eco72I CACGTG 1 cut(s) 1168
Eco88I CYCGRG 1 cut(s) 32
EcoICRI GAGCTC 1 cut(s) 37
EcoNI CCTNNNNNAGG 1 cut(s) 920
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 599
EcoT38I GRGCYC 2 cut(s) 29, 39
FaeI CATG 5 cut(s) 68, 129, 255, 1043, 1090
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FaqI GGGAC 3 cut(s) 294, 842, 1277
FatI CATG 5 cut(s) 64, 125, 251, 1039, 1086
Fnu4HI GCNGC 2 cut(s) 612, 951
FokI GGATG 1 cut(s) 145
FriOI GRGCYC 2 cut(s) 29, 39
Fsp4HI GCNGC 2 cut(s) 612, 951
FspBI CTAG 3 cut(s) 374, 438, 1251
FspI TGCGCA 1 cut(s) 920
GlaI GCGC 3 cut(s) 864, 920, 999
GluI GCNGC 2 cut(s) 612, 951
HaeII RGCGCY 1 cut(s) 1001
HaeIII GGCC 2 cut(s) 27, 313
HapII CCGG 2 cut(s) 638, 1295
HhaI GCGC 3 cut(s) 865, 921, 1000
Hin1II CATG 5 cut(s) 68, 129, 255, 1043, 1090
Hin6I GCGC 3 cut(s) 863, 919, 998
HinP1I GCGC 3 cut(s) 863, 919, 998
HindIII AAGCTT 2 cut(s) 1103, 1154
HinfI GANTC 7 cut(s) 121, 256, 282, 430, 452, 767, 1081
HpaII CCGG 2 cut(s) 638, 1295
HphI GGTGA 3 cut(s) 145, 1154, 1154
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 6 cut(s) 166, 299, 670, 697, 718, 1268
Hpy188III TCNNGA 5 cut(s) 260, 854, 884, 1040, 1078
Hpy8I GTNNAC 1 cut(s) 352
HpyAV CCTTC 7 cut(s) 217, 349, 547, 556, 1180, 1312, 1318
HpyCH4III ACNGT 1 cut(s) 203
HpyCH4IV ACGT 3 cut(s) 390, 831, 1167
HpyCH4V TGCA 5 cut(s) 448, 525, 655, 1099, 1280
HpyF10VI GCNNNNNNNGC 4 cut(s) 531, 611, 869, 1004
HpyF3I CTNAG 3 cut(s) 163, 296, 1265
HpySE526I ACGT 3 cut(s) 390, 831, 1167
Hsp92II CATG 5 cut(s) 68, 129, 255, 1043, 1090
HspAI GCGC 3 cut(s) 863, 919, 998
KpnI GGTACC 1 cut(s) 628
Kzo9I GATC 6 cut(s) 166, 226, 570, 658, 850, 886
LmnI GCTCC 2 cut(s) 10, 42
Lsp1109I GCAGC 1 cut(s) 937
LweI GCATC 1 cut(s) 701
MaeI CTAG 3 cut(s) 374, 438, 1251
MaeII ACGT 3 cut(s) 390, 831, 1167
MaeIII GTNAC 2 cut(s) 203, 1201
MalI GATC 6 cut(s) 168, 228, 572, 660, 852, 888
MboI GATC 6 cut(s) 166, 226, 570, 658, 850, 886
MfeI CAATTG 1 cut(s) 762
MflI RGATCY 3 cut(s) 166, 226, 850
MhlI GDGCHC 2 cut(s) 29, 39
MluCI AATT 9 cut(s) 12, 134, 594, 749, 762, 1067, 1073, 1122, 1270
MlyI GAGTC 3 cut(s) 276, 446, 1090
MmeI TCCRAC 2 cut(s) 405, 479
MseI TTAA 2 cut(s) 144, 701
MslI CAYNNNNRTG 2 cut(s) 660, 1044
MspI CCGG 2 cut(s) 638, 1295
MspR9I CCNGG 3 cut(s) 601, 638, 1295
MunI CAATTG 1 cut(s) 762
MvaI CCWGG 1 cut(s) 601
MvnI CGCG 1 cut(s) 885
MwoI GCNNNNNNNGC 4 cut(s) 531, 611, 869, 1004
NciI CCSGG 2 cut(s) 638, 1295
NdeII GATC 6 cut(s) 166, 226, 570, 658, 850, 886
NlaIII CATG 5 cut(s) 68, 129, 255, 1043, 1090
NlaIV GGNNCC 4 cut(s) 6, 27, 350, 626
NruI TCGCGA 1 cut(s) 885
NsbI TGCGCA 1 cut(s) 920
OliI CACNNNNGTG 1 cut(s) 660
PagI TCATGA 1 cut(s) 1039
PfeI GAWTC 4 cut(s) 121, 256, 430, 767
PflMI CCANNNNNTGG 1 cut(s) 1171
PfoI TCCNGGA 1 cut(s) 1293
PkrI GCNGC 2 cut(s) 613, 952
Ple19I CGATCG 2 cut(s) 661, 889
PleI GAGTC 3 cut(s) 276, 446, 1089
PmaCI CACGTG 1 cut(s) 1168
PmlI CACGTG 1 cut(s) 1168
PpsI GAGTC 3 cut(s) 276, 446, 1089
Ppu21I YACGTR 1 cut(s) 1168
Psp124BI GAGCTC 1 cut(s) 39
Psp1406I AACGTT 1 cut(s) 390
Psp6I CCWGG 1 cut(s) 599
PspCI CACGTG 1 cut(s) 1168
PspGI CCWGG 1 cut(s) 599
PspN4I GGNNCC 4 cut(s) 6, 27, 350, 626
PspOMI GGGCCC 1 cut(s) 25
PspPI GGNCC 5 cut(s) 25, 26, 312, 349, 516
PsuI RGATCY 3 cut(s) 166, 226, 850
PvuI CGATCG 2 cut(s) 661, 889
RruI TCGCGA 1 cut(s) 885
RsaI GTAC 3 cut(s) 626, 845, 906
RsaNI GTAC 3 cut(s) 625, 844, 905
RseI CAYNNNNRTG 2 cut(s) 660, 1044
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 2 cut(s) 144, 701
SatI GCNGC 2 cut(s) 612, 951
Sau3AI GATC 6 cut(s) 166, 226, 570, 658, 850, 886
Sau96I GGNCC 5 cut(s) 25, 26, 312, 349, 516
ScaI AGTACT 1 cut(s) 906
SchI GAGTC 3 cut(s) 276, 446, 1090
ScrFI CCNGG 3 cut(s) 601, 638, 1295
SduI GDGCHC 2 cut(s) 29, 39
SfaNI GCATC 1 cut(s) 701
SfcI CTRYAG 1 cut(s) 1018
SinI GGWCC 2 cut(s) 349, 516
SmiMI CAYNNNNRTG 2 cut(s) 660, 1044
Sse9I AATT 9 cut(s) 12, 134, 594, 749, 762, 1067, 1073, 1122, 1270
SsiI CCGC 5 cut(s) 40, 57, 611, 991, 1212
SspI AATATT 1 cut(s) 1093
SspMI CTAG 3 cut(s) 374, 438, 1251
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 3 cut(s) 599, 636, 1293
TaaI ACNGT 1 cut(s) 203
TaiI ACGT 3 cut(s) 393, 834, 1170
TaqI TCGA 1 cut(s) 1128
TasI AATT 9 cut(s) 12, 134, 594, 749, 762, 1067, 1073, 1122, 1270
TatI WGTACW 2 cut(s) 843, 904
TauI GCSGC 1 cut(s) 614
TfiI GAWTC 4 cut(s) 121, 256, 430, 767
Tru1I TTAA 2 cut(s) 144, 701
Tru9I TTAA 2 cut(s) 144, 701
TscAI CASTG 4 cut(s) 939, 951, 1229, 1287
TseI GCWGC 1 cut(s) 950
TspDTI ATGAA 5 cut(s) 81, 503, 543, 852, 1103
TspRI CASTG 4 cut(s) 939, 951, 1229, 1287
Van91I CCANNNNNTGG 1 cut(s) 1171
VpaK11BI GGWCC 2 cut(s) 349, 516
XagI CCTNNNNNAGG 1 cut(s) 920
XapI RAATTY 3 cut(s) 134, 749, 1073
XspI CTAG 3 cut(s) 374, 438, 1251
ZrmI AGTACT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.