RchiOBHm_Chr6g0254021

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001292
Physical Location & Seq
Forward (+)
9100441 .. 9101355
915 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22782

Sequence Viewer

Length: 915 bp
ATGGCTTCTCAGTTTTGTCTACTAGGGCCGTCGGAGCTCCGCCGCCCTAACCTCCCCAAAACCAACCGAACCCTTATGACAACCTCAGAATATCAGGACCCTTCGCTCTCAGACTTAAAGCCAATTTTAGAGTTTATCTTCGAGGATGCGAATTACGCTGGAAAGCCGGCTGTTCCGGCAGAGAGAATTTCCAAATATAAGCTGGATGACAGAGGGGAAACGGCTTGGAAGGTTGGCCCTCTCAAAACCCTCAAGCTTATATTGAGTCTCAGAGCGGCGGGGAAGCTTGACGATGATGGGTTTTACAGAACCCTGTTGTGGGTCCACAAAAACCATCCTTTAACCCTAGCTTTGAATCTCAAGGTTTTTGGGGATTTAGGGTCGTTCAAAGATGTGCTGGGAATTCTCCATAAGGTTTTGGAGGAATCCATATGTGGACATTATCTGTGGACTCCATATGGGTACTGGTGGACTCCATATGGGTACTATTTTTCATTCCTTGAAGGGTACGAGCGTTTAGAGAAGGAAGGGTACCCTTGGTCAGGATACGGGAAGGAAAAAGAGGAGAAAAATAAAGTTGAAGAGGTAGTTGTAGCATGGAGAAAAGCAGATGACATTTCAAAGGCTAAAATTGCAGTTGAGAGGTACCAAAATGACCAAAGGTATCGAAACTTGCATTATCAAGTAGCTGATTTGTTTGCAGGGTTTTTGAAATCTGATCTCAAATTTTTGGAATCAGGTGAGATTGAAAACATCAGTTTTGCAGCCAAATTTTGCCCTTCAATTGGTTCCAAATATGATAGAGAAACCTTGATATGTGAAGTCATTGCAAAGAGAATTTTCCCACGTGACGATTATGAAGAGTACAAAGATATTTGGAACCCCATTATGGTTACAGGGTTCGTGATCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

304

Amino Acids

35.23

Weight (kDa)

6.27

Isoelectric Point (pI)

30.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 63 - 289 3.6e-44 Domain of unknown function (DUF2828)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 531, 645
AccB1I GGYRCC 2 cut(s) 531, 645
AccBSI CCGCTC 1 cut(s) 275
AccI GTMKAC 1 cut(s) 19
AciI CCGC 4 cut(s) 40, 43, 275, 278
AcsI RAATTY 5 cut(s) 186, 402, 725, 770, 837
AcvI CACGTG 1 cut(s) 848
AfaI GTAC 6 cut(s) 464, 485, 509, 533, 647, 866
AfiI CCNNNNNNNGG 5 cut(s) 318, 319, 542, 785, 889
AgsI TTSAA 8 cut(s) 355, 388, 503, 581, 621, 712, 749, 783
AluBI AGCT 6 cut(s) 37, 202, 256, 286, 350, 689
AluI AGCT 6 cut(s) 37, 202, 256, 286, 350, 689
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 272
AoxI GGCC 2 cut(s) 26, 235
ApeKI GCWGC 1 cut(s) 764
ApoI RAATTY 5 cut(s) 186, 402, 725, 770, 837
Asp718I GGTACC 2 cut(s) 531, 645
AspS9I GGNCC 4 cut(s) 26, 97, 236, 322
AsuHPI GGTGA 1 cut(s) 752
AvaII GGWCC 2 cut(s) 97, 322
BaeI ACNNNNGTAYC 2 cut(s) 499, 532
BanI GGYRCC 2 cut(s) 531, 645
BanII GRGCYC 1 cut(s) 39
BarI GAAGNNNNNNTAC 2 cut(s) 515, 547
BbrPI CACGTG 1 cut(s) 848
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 1 cut(s) 776
BccI CCATC 2 cut(s) 290, 342
BceAI ACGGC 2 cut(s) 13, 237
BciVI GTATCC 1 cut(s) 539
BcoDI GTCTC 1 cut(s) 272
BfaI CTAG 2 cut(s) 23, 347
BfuI GTATCC 1 cut(s) 539
BisI GCNGC 3 cut(s) 43, 276, 765
BlsI GCNGC 3 cut(s) 44, 277, 766
Bme18I GGWCC 2 cut(s) 97, 322
BmgT120I GGNCC 4 cut(s) 26, 97, 236, 322
BmiI GGNNCC 6 cut(s) 99, 323, 533, 647, 790, 881
BmsI GCATC 1 cut(s) 136
BpuEI CTTGAG 2 cut(s) 236, 344
BsaAI YACGTR 1 cut(s) 848
BsaJI CCNNGG 1 cut(s) 536
Bsc4I CCNNNNNNNGG 5 cut(s) 318, 319, 542, 785, 889
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 1 cut(s) 470
Bse3DI GCAATG 1 cut(s) 825
BseDI CCNNGG 1 cut(s) 536
BseGI GGATG 3 cut(s) 151, 211, 334
BseLI CCNNNNNNNGG 5 cut(s) 318, 319, 542, 785, 889
BseMI GCAATG 1 cut(s) 825
BseMII CTCAG 4 cut(s) 23, 99, 123, 283
BseNI ACTGG 1 cut(s) 470
BseRI GAGGAG 1 cut(s) 578
BseXI GCAGC 1 cut(s) 776
BseYI CCCAGC 1 cut(s) 397
BshFI GGCC 2 cut(s) 28, 237
BshNI GGYRCC 2 cut(s) 531, 645
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 2 cut(s) 167, 176
BslI CCNNNNNNNGG 5 cut(s) 318, 319, 542, 785, 889
BsmAI GTCTC 1 cut(s) 272
BsnI GGCC 2 cut(s) 28, 237
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 2 cut(s) 718, 906
BspACI CCGC 4 cut(s) 40, 43, 275, 278
BspANI GGCC 2 cut(s) 28, 237
BspCNI CTCAG 4 cut(s) 22, 98, 122, 282
BspLI GGNNCC 6 cut(s) 99, 323, 533, 647, 790, 881
BspT107I GGYRCC 2 cut(s) 531, 645
BsrBI CCGCTC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 825
BsrFI RCCGGY 1 cut(s) 166
BsrI ACTGG 1 cut(s) 470
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 536
BssMI GATC 2 cut(s) 718, 906
BssT1I CCWWGG 1 cut(s) 536
Bst6I CTCTTC 2 cut(s) 576, 855
BstBAI YACGTR 1 cut(s) 848
BstC8I GCNNGC 1 cut(s) 168
BstDEI CTNAG 4 cut(s) 9, 85, 109, 269
BstENI CCTNNNNNAGG 1 cut(s) 540
BstF5I GGATG 3 cut(s) 151, 211, 334
BstKTI GATC 2 cut(s) 721, 909
BstMAI GTCTC 1 cut(s) 272
BstMBI GATC 2 cut(s) 718, 906
BstMWI GCNNNNNNNGC 4 cut(s) 34, 155, 176, 632
BstV1I GCAGC 1 cut(s) 776
BsuI GTATCC 1 cut(s) 539
BsuRI GGCC 2 cut(s) 28, 237
BtsCI GGATG 3 cut(s) 151, 211, 334
Cac8I GCNNGC 1 cut(s) 168
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 4 cut(s) 26, 97, 236, 322
Csp6I GTAC 6 cut(s) 463, 484, 508, 532, 646, 865
CviAII CATG 1 cut(s) 597
CviQI GTAC 6 cut(s) 463, 484, 508, 532, 646, 865
DdeI CTNAG 4 cut(s) 9, 85, 109, 269
DpnI GATC 2 cut(s) 720, 908
DpnII GATC 2 cut(s) 718, 906
Eam1104I CTCTTC 2 cut(s) 576, 855
EarI CTCTTC 2 cut(s) 576, 855
EciI GGCGGA 1 cut(s) 29
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 536
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 2 cut(s) 97, 322
Eco53kI GAGCTC 1 cut(s) 37
Eco72I CACGTG 1 cut(s) 848
EcoICRI GAGCTC 1 cut(s) 37
EcoNI CCTNNNNNAGG 1 cut(s) 540
EcoO109I RGGNCCY 1 cut(s) 97
EcoRI GAATTC 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 536
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 536
FaeI CATG 1 cut(s) 600
FatI CATG 1 cut(s) 596
FauI CCCGC 1 cut(s) 271
FauNDI CATATG 3 cut(s) 431, 457, 478
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 3 cut(s) 43, 276, 765
FokI GGATG 3 cut(s) 158, 218, 321
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 3 cut(s) 43, 276, 765
FspBI CTAG 2 cut(s) 23, 347
GluI GCNGC 3 cut(s) 43, 276, 765
GsaI CCCAGC 1 cut(s) 401
HaeIII GGCC 2 cut(s) 28, 237
HapII CCGG 2 cut(s) 167, 176
Hin1II CATG 1 cut(s) 600
HindIII AAGCTT 2 cut(s) 254, 284
HinfI GANTC 6 cut(s) 265, 355, 425, 451, 472, 734
HpaII CCGG 2 cut(s) 167, 176
HphI GGTGA 1 cut(s) 752
Hpy166II GTNNAC 5 cut(s) 20, 325, 437, 450, 471
Hpy188I TCNGA 5 cut(s) 34, 88, 112, 272, 718
Hpy188III TCNNGA 3 cut(s) 95, 543, 904
Hpy8I GTNNAC 5 cut(s) 20, 325, 437, 450, 471
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 7 cut(s) 111, 223, 497, 517, 521, 547, 789
HpyCH4IV ACGT 1 cut(s) 847
HpyCH4V TGCA 5 cut(s) 635, 676, 701, 764, 830
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 155, 176, 632
HpyF3I CTNAG 4 cut(s) 9, 85, 109, 269
HpySE526I ACGT 1 cut(s) 847
Hsp92II CATG 1 cut(s) 600
KpnI GGTACC 2 cut(s) 535, 649
KroI GCCGGC 1 cut(s) 166
KroNI GCCGGC 1 cut(s) 168
Kzo9I GATC 2 cut(s) 718, 906
LmnI GCTCC 2 cut(s) 34, 42
Lsp1109I GCAGC 1 cut(s) 776
LweI GCATC 1 cut(s) 136
MaeI CTAG 2 cut(s) 23, 347
MaeII ACGT 1 cut(s) 847
MaeIII GTNAC 2 cut(s) 848, 892
MalI GATC 2 cut(s) 720, 908
MbiI CCGCTC 1 cut(s) 275
MboI GATC 2 cut(s) 718, 906
MboII GAAGA 3 cut(s) 130, 593, 872
MfeI CAATTG 1 cut(s) 783
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 9 cut(s) 123, 151, 186, 402, 630, 725, 770, 783, 837
MlyI GAGTC 3 cut(s) 274, 445, 466
MmeI TCCRAC 1 cut(s) 12
MroNI GCCGGC 1 cut(s) 166
MseI TTAA 2 cut(s) 116, 341
MspI CCGG 2 cut(s) 167, 176
MunI CAATTG 1 cut(s) 783
MwoI GCNNNNNNNGC 4 cut(s) 34, 155, 176, 632
NaeI GCCGGC 1 cut(s) 168
NdeI CATATG 3 cut(s) 431, 457, 478
NdeII GATC 2 cut(s) 718, 906
NgoMIV GCCGGC 1 cut(s) 166
NlaIII CATG 1 cut(s) 600
NlaIV GGNNCC 6 cut(s) 99, 323, 533, 647, 790, 881
NmuCI GTSAC 1 cut(s) 848
PdiI GCCGGC 1 cut(s) 168
PfeI GAWTC 3 cut(s) 355, 425, 734
PkrI GCNGC 3 cut(s) 44, 277, 766
PleI GAGTC 3 cut(s) 273, 445, 466
PmaCI CACGTG 1 cut(s) 848
PmlI CACGTG 1 cut(s) 848
PpsI GAGTC 3 cut(s) 273, 445, 466
Ppu21I YACGTR 1 cut(s) 848
PpuMI RGGWCCY 1 cut(s) 97
Psp124BI GAGCTC 1 cut(s) 39
Psp5II RGGWCCY 1 cut(s) 97
PspCI CACGTG 1 cut(s) 848
PspFI CCCAGC 1 cut(s) 397
PspN4I GGNNCC 6 cut(s) 99, 323, 533, 647, 790, 881
PspPI GGNCC 4 cut(s) 26, 97, 236, 322
PspPPI RGGWCCY 1 cut(s) 97
RsaI GTAC 6 cut(s) 464, 485, 509, 533, 647, 866
RsaNI GTAC 6 cut(s) 463, 484, 508, 532, 646, 865
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 2 cut(s) 116, 341
SatI GCNGC 3 cut(s) 43, 276, 765
Sau3AI GATC 2 cut(s) 718, 906
Sau96I GGNCC 4 cut(s) 26, 97, 236, 322
SchI GAGTC 3 cut(s) 274, 445, 466
SduI GDGCHC 1 cut(s) 39
SfaNI GCATC 1 cut(s) 136
SinI GGWCC 2 cut(s) 97, 322
SmlI CTYRAG 2 cut(s) 251, 359
SmoI CTYRAG 2 cut(s) 251, 359
Sse9I AATT 9 cut(s) 123, 151, 186, 402, 630, 725, 770, 783, 837
SsiI CCGC 4 cut(s) 40, 43, 275, 278
SspMI CTAG 2 cut(s) 23, 347
SstI GAGCTC 1 cut(s) 39
StyI CCWWGG 1 cut(s) 536
TaiI ACGT 1 cut(s) 850
TaqI TCGA 2 cut(s) 141, 667
TasI AATT 9 cut(s) 123, 151, 186, 402, 630, 725, 770, 783, 837
TatI WGTACW 1 cut(s) 864
TauI GCSGC 2 cut(s) 45, 278
TfiI GAWTC 3 cut(s) 355, 425, 734
Tru1I TTAA 2 cut(s) 116, 341
Tru9I TTAA 2 cut(s) 116, 341
TseFI GTSAC 1 cut(s) 848
TseI GCWGC 1 cut(s) 764
Tsp45I GTSAC 1 cut(s) 848
TspDTI ATGAA 2 cut(s) 483, 873
VpaK11BI GGWCC 2 cut(s) 97, 322
XagI CCTNNNNNAGG 1 cut(s) 540
XapI RAATTY 5 cut(s) 186, 402, 725, 770, 837
XcmI CCANNNNNNNNNTGG 2 cut(s) 199, 462
XmiI GTMKAC 1 cut(s) 19
XspI CTAG 2 cut(s) 23, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.