Rmu_sc0001716.1_g000026

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001716.1
OG0001292
Physical Location & Seq
Forward (+)
91972 .. 94663
2692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001716.1_g000026.1.cds

Sequence Viewer

Length: 1881 bp
atggcttctccgttttgtctcctagggccttcggagctccgccgccctaacctccccaaaaacaaccgaaccctaatgacaacctctgaacaccaggaccattcggtctcacgcttaaagccactcttagagtttgtcttcaacgatcgatgcaccagaggggatacgacttggaacgttgaccctctcaaaaccctcaagcttatatttagtcttagagcggcggggaagatcaacgatgatgggttttacagaaccatgttgtgggtccacaaaaaccatcctttaaccctagctttgaatctcagggtttttggggatttagggctgttcaaagatctgctgggaattctccataaggtattggaggaatccatatgtggacactatctgtggactccatatgggtactggtggactccaaatgggtactatttttcattccttgaagggtaccagtgtttagagaaggaagagttctattgggaaaaagagaggagaaataaagttgaagtcgtagttgcagcatggagaagagcagctgacatttcaaaggctaaaattgcagttgagaggtaccaaaatgaccaaaggtatcaaaacttgcataatcaagtagcggatttgttttcagagttcttgaaatctgatgtcaaatttttggaatcaggtgagattgaaaacatcagttttgctgccaaattttgcccttcaattgattccaaatatgataaagcaaccctgatatgtgaaaaacaagtgcttgttcctctgcacaaagtgctacagtcatcatcagtgaacaatgctgttgagagaaagaagtcatggtcccaaaaccttaatgccctgaattgtttggaaacttattgggaaatctacaattataggtgctccagattagaactatttaagctttacatgaagattatggacaggttgggcataaaaattgatgacaggctaaaacttcctcaccaagtaatagcttttttgaacaatacacagggtagtgatgaaattgcagagcttcagtggcagaaactagtccaagggctgtcaaacaaagggaagctcaggaattgtcttgctgtgtgtgacagctcgttgagcatgataggaacagacaaggaaatggcgtgcattgcaatgggattattgatttcagaactgagcgagagtccatggaatggagtggttttctcattcactgattttcccaaaattcacaagattggaggagaaagtcttcgattgaagtccgagttcatgaggcagatggagtgtgatgagaaagtggactgcttagagatttacaaccgagtgttggagattgccaccacacaaaagctaaaccacataaagatgccaaagaggatttttgtgttcacctacaaggattttgttgaggccttcaagtatgattggctggatgattacacagaagcatataacaatttcagaaaaagagggtttgagtttaatgtgccagagctagtgttttggaatttgaaggattccattgccaagccagaggtgatttatagcccagtgaaggagaaccagaaagtggggatcataataacagggttctctagcaatctactcaggttgttcttggaaggggaggcagattcaagatcatatgcagctcaattccaggcaccttacggtattgatttacagtcagtaccaaaactgcttcctagagtagaggatgtgtttgatagtgcaatttcaagagaagaattagagagcctggtcttctcagttcaagctgctgccatggagattctctcggcagttgccatgactaaagaaattcagccttgcttatctttagtaattcaccgtttgatgttgggaacaataaatacttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

626

Amino Acids

72.4

Weight (kDa)

6.87

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 104
Acc65I GGTACC 2 cut(s) 453, 576
AccB1I GGYRCC 3 cut(s) 453, 576, 1663
AccB7I CCANNNNNTGG 3 cut(s) 264, 1190, 1570
AccBSI CCGCTC 1 cut(s) 221
AciI CCGC 5 cut(s) 40, 43, 221, 224, 620
AclI AACGTT 1 cut(s) 177
AclWI GGATC 1 cut(s) 1583
AcsI RAATTY 6 cut(s) 348, 656, 701, 1224, 1507, 1821
AcuI CTGAAG 1 cut(s) 1016
AdeI CACNNNGTG 1 cut(s) 781
AfaI GTAC 5 cut(s) 410, 431, 455, 578, 1692
AfiI CCNNNNNNNGG 5 cut(s) 264, 1190, 1327, 1555, 1570
AhlI ACTAGT 1 cut(s) 1045
AjnI CCWGG 3 cut(s) 93, 1659, 1758
Alw21I GWGCWC 2 cut(s) 39, 896
Alw26I GTCTC 2 cut(s) 23, 112
AlwI GGATC 1 cut(s) 1583
AoxI GGCC 2 cut(s) 26, 1410
ApeKI GCWGC 6 cut(s) 524, 539, 695, 1649, 1778, 1781
ApoI RAATTY 6 cut(s) 348, 656, 701, 1224, 1507, 1821
Asp700I GAANNNNTTC 1 cut(s) 1248
Asp718I GGTACC 2 cut(s) 453, 576
AspA2I CCTAGG 1 cut(s) 22
AspS9I GGNCC 4 cut(s) 26, 97, 268, 831
AsuHPI GGTGA 5 cut(s) 683, 968, 1381, 1549, 1841
AvaII GGWCC 3 cut(s) 97, 268, 831
AvrII CCTAGG 1 cut(s) 22
BaeI ACNNNNGTAYC 2 cut(s) 445, 478
BanI GGYRCC 3 cut(s) 453, 576, 1663
BanII GRGCYC 1 cut(s) 39
BbsI GAAGAC 3 cut(s) 130, 1241, 1756
Bbv12I GWGCWC 2 cut(s) 39, 896
BbvI GCAGC 6 cut(s) 536, 551, 682, 1661, 1765, 1768
BccI CCATC 3 cut(s) 236, 288, 1273
BciT130I CCWGG 3 cut(s) 95, 1661, 1760
BciVI GTATCC 1 cut(s) 157
BcoDI GTCTC 2 cut(s) 23, 112
BcuI ACTAGT 1 cut(s) 1045
BfaI CTAG 6 cut(s) 23, 293, 1046, 1496, 1596, 1707
BfmI CTRYAG 1 cut(s) 785
BfuI GTATCC 1 cut(s) 157
BglII AGATCT 1 cut(s) 337
BisI GCNGC 8 cut(s) 43, 222, 525, 540, 696, 1650, 1779, 1782
BlnI CCTAGG 1 cut(s) 22
BlsI GCNGC 8 cut(s) 44, 223, 526, 541, 697, 1651, 1780, 1783
Bme1390I CCNGG 3 cut(s) 95, 1661, 1760
Bme18I GGWCC 3 cut(s) 97, 268, 831
BmgT120I GGNCC 4 cut(s) 26, 97, 268, 831
BmiI GGNNCC 5 cut(s) 269, 455, 578, 833, 1665
BmrFI CCNGG 3 cut(s) 95, 1661, 1760
BmrI ACTGGG 1 cut(s) 1544
BmsI GCATC 2 cut(s) 140, 1356
BmuI ACTGGG 1 cut(s) 1544
BpiI GAAGAC 3 cut(s) 130, 1241, 1756
BplI GAGNNNNNCTC 2 cut(s) 1781, 1813
BpmI CTGGAG 1 cut(s) 880
Bpu10I CCTNAGC 1 cut(s) 1076
BpuEI CTTGAG 1 cut(s) 182
Bsa29I ATCGAT 1 cut(s) 148
BsaI GGTCTC 1 cut(s) 112
BsaJI CCNNGG 4 cut(s) 22, 1051, 1184, 1785
Bsc4I CCNNNNNNNGG 5 cut(s) 264, 1190, 1327, 1555, 1570
Bse1I ACTGG 3 cut(s) 416, 457, 1550
Bse3DI GCAATG 3 cut(s) 1143, 1155, 1521
BseBI CCWGG 3 cut(s) 95, 1661, 1760
BseCI ATCGAT 1 cut(s) 148
BseDI CCNNGG 4 cut(s) 22, 1051, 1184, 1785
BseGI GGATG 3 cut(s) 280, 1438, 1723
BseLI CCNNNNNNNGG 5 cut(s) 264, 1190, 1327, 1555, 1570
BseMI GCAATG 3 cut(s) 1143, 1155, 1521
BseMII CTCAG 5 cut(s) 319, 1090, 1163, 1621, 1782
BseNI ACTGG 3 cut(s) 416, 457, 1550
BseRI GAGGAG 2 cut(s) 511, 1254
BseXI GCAGC 6 cut(s) 536, 551, 682, 1661, 1765, 1768
BseYI CCCAGC 1 cut(s) 343
BsgI GTGCAG 1 cut(s) 758
Bsh1285I CGRYCG 1 cut(s) 148
BshFI GGCC 2 cut(s) 28, 1412
BshNI GGYRCC 3 cut(s) 453, 576, 1663
BshVI ATCGAT 1 cut(s) 148
BsiEI CGRYCG 1 cut(s) 148
BsiHKAI GWGCWC 2 cut(s) 39, 896
BslFI GGGAC 1 cut(s) 817
BslI CCNNNNNNNGG 5 cut(s) 264, 1190, 1327, 1555, 1570
BsmAI GTCTC 2 cut(s) 23, 112
BsmFI GGGAC 1 cut(s) 817
BsnI GGCC 2 cut(s) 28, 1412
Bso31I GGTCTC 1 cut(s) 112
Bsp1286I GDGCHC 2 cut(s) 39, 896
Bsp143I GATC 5 cut(s) 145, 231, 337, 1575, 1640
Bsp19I CCATGG 2 cut(s) 1184, 1785
BspACI CCGC 5 cut(s) 40, 43, 221, 224, 620
BspANI GGCC 2 cut(s) 28, 1412
BspCNI CTCAG 5 cut(s) 318, 1089, 1164, 1620, 1781
BspDI ATCGAT 1 cut(s) 148
BspHI TCATGA 1 cut(s) 1269
BspLI GGNNCC 5 cut(s) 269, 455, 578, 833, 1665
BspPI GGATC 1 cut(s) 1583
BspQI GCTCTTC 1 cut(s) 529
BspT107I GGYRCC 3 cut(s) 453, 576, 1663
BspTNI GGTCTC 1 cut(s) 112
BsrBI CCGCTC 1 cut(s) 221
BsrDI GCAATG 3 cut(s) 1143, 1155, 1521
BsrI ACTGG 3 cut(s) 416, 457, 1550
BssECI CCNNGG 4 cut(s) 22, 1051, 1184, 1785
BssMI GATC 5 cut(s) 145, 231, 337, 1575, 1640
BssT1I CCWWGG 4 cut(s) 22, 1051, 1184, 1785
Bst2UI CCWGG 3 cut(s) 95, 1661, 1760
Bst4CI ACNGT 4 cut(s) 789, 1673, 1686, 1853
Bst6I CTCTTC 2 cut(s) 468, 529
BstAPI GCANNNNNTGC 1 cut(s) 781
BstC8I GCNNGC 1 cut(s) 1141
BstDEI CTNAG 8 cut(s) 127, 215, 305, 1076, 1172, 1306, 1607, 1768
BstDSI CCRYGG 2 cut(s) 1184, 1785
BstF5I GGATG 3 cut(s) 280, 1438, 1723
BstKTI GATC 5 cut(s) 148, 234, 340, 1578, 1643
BstMAI GTCTC 2 cut(s) 23, 112
BstMBI GATC 5 cut(s) 145, 231, 337, 1575, 1640
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 6 cut(s) 34, 563, 781, 1036, 1110, 1145
BstNI CCWGG 3 cut(s) 95, 1661, 1760
BstSCI CCNGG 3 cut(s) 93, 1659, 1758
BstSFI CTRYAG 1 cut(s) 785
BstV1I GCAGC 6 cut(s) 536, 551, 682, 1661, 1765, 1768
BstV2I GAAGAC 3 cut(s) 130, 1241, 1756
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
Bsu15I ATCGAT 1 cut(s) 148
BsuI GTATCC 1 cut(s) 157
BsuRI GGCC 2 cut(s) 28, 1412
BsuTUI ATCGAT 1 cut(s) 148
BtgI CCRYGG 2 cut(s) 1184, 1785
BtsCI GGATG 3 cut(s) 280, 1438, 1723
BtsIMutI CAGTG 5 cut(s) 464, 804, 1040, 1209, 1557
Cac8I GCNNGC 1 cut(s) 1141
CciI TCATGA 1 cut(s) 1269
Cfr13I GGNCC 4 cut(s) 26, 97, 268, 831
ClaI ATCGAT 1 cut(s) 148
Csp6I GTAC 5 cut(s) 409, 430, 454, 577, 1691
CviAII CATG 9 cut(s) 259, 528, 828, 922, 1114, 1185, 1270, 1786, 1810
CviQI GTAC 5 cut(s) 409, 430, 454, 577, 1691
DdeI CTNAG 8 cut(s) 127, 215, 305, 1076, 1172, 1306, 1607, 1768
DpnI GATC 5 cut(s) 147, 233, 339, 1577, 1642
DpnII GATC 5 cut(s) 145, 231, 337, 1575, 1640
DraIII CACNNNGTG 1 cut(s) 781
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
Eam1104I CTCTTC 2 cut(s) 468, 529
EarI CTCTTC 2 cut(s) 468, 529
EciI GGCGGA 1 cut(s) 29
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 4 cut(s) 22, 1051, 1184, 1785
Eco147I AGGCCT 1 cut(s) 1412
Eco24I GRGCYC 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 112
Eco47I GGWCC 3 cut(s) 97, 268, 831
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 1016
EcoICRI GAGCTC 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 26
EcoRI GAATTC 1 cut(s) 348
EcoRII CCWGG 3 cut(s) 93, 1659, 1758
EcoT14I CCWWGG 4 cut(s) 22, 1051, 1184, 1785
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 4 cut(s) 22, 1051, 1184, 1785
FaeI CATG 9 cut(s) 262, 531, 831, 925, 1117, 1188, 1273, 1789, 1813
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FaqI GGGAC 1 cut(s) 817
FatI CATG 9 cut(s) 258, 527, 827, 921, 1113, 1184, 1269, 1785, 1809
FauI CCCGC 1 cut(s) 217
FauNDI CATATG 3 cut(s) 377, 403, 1645
Fnu4HI GCNGC 8 cut(s) 43, 222, 525, 540, 696, 1650, 1779, 1782
FokI GGATG 3 cut(s) 267, 1445, 1730
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 8 cut(s) 43, 222, 525, 540, 696, 1650, 1779, 1782
FspBI CTAG 6 cut(s) 23, 293, 1046, 1496, 1596, 1707
GluI GCNGC 8 cut(s) 43, 222, 525, 540, 696, 1650, 1779, 1782
GsaI CCCAGC 1 cut(s) 347
GsuI CTGGAG 1 cut(s) 880
HaeIII GGCC 2 cut(s) 28, 1412
Hin1II CATG 9 cut(s) 262, 531, 831, 925, 1117, 1188, 1273, 1789, 1813
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 2 cut(s) 200, 914
HphI GGTGA 5 cut(s) 683, 968, 1381, 1549, 1841
Hpy166II GTNNAC 8 cut(s) 181, 271, 383, 396, 417, 802, 1300, 1389
Hpy188I TCNGA 7 cut(s) 34, 88, 634, 649, 1168, 1264, 1463
Hpy188III TCNNGA 6 cut(s) 640, 897, 1078, 1270, 1638, 1740
Hpy8I GTNNAC 8 cut(s) 181, 271, 383, 396, 417, 802, 1300, 1389
HpyAV CCTTC 8 cut(s) 39, 443, 463, 720, 1423, 1507, 1549, 1616
HpyCH4III ACNGT 4 cut(s) 789, 1673, 1686, 1853
HpyCH4IV ACGT 1 cut(s) 177
HpyF10VI GCNNNNNNNGC 6 cut(s) 34, 563, 781, 1036, 1110, 1145
HpyF3I CTNAG 8 cut(s) 127, 215, 305, 1076, 1172, 1306, 1607, 1768
HpySE526I ACGT 1 cut(s) 177
Hsp92II CATG 9 cut(s) 262, 531, 831, 925, 1117, 1188, 1273, 1789, 1813
KpnI GGTACC 2 cut(s) 457, 580
Kzo9I GATC 5 cut(s) 145, 231, 337, 1575, 1640
LguI GCTCTTC 1 cut(s) 529
LmnI GCTCC 3 cut(s) 34, 42, 899
Lsp1109I GCAGC 6 cut(s) 536, 551, 682, 1661, 1765, 1768
LweI GCATC 2 cut(s) 140, 1356
MaeI CTAG 6 cut(s) 23, 293, 1046, 1496, 1596, 1707
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 1 cut(s) 1097
MalI GATC 5 cut(s) 147, 233, 339, 1577, 1642
MbiI CCGCTC 1 cut(s) 221
MboI GATC 5 cut(s) 145, 231, 337, 1575, 1640
MboII GAAGA 8 cut(s) 130, 241, 485, 546, 937, 1241, 1756, 1757
MfeI CAATTG 1 cut(s) 714
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 2 cut(s) 39, 896
MlyI GAGTC 3 cut(s) 391, 412, 1189
MmeI TCCRAC 1 cut(s) 1308
MroXI GAANNNNTTC 1 cut(s) 1248
MseI TTAA 6 cut(s) 116, 287, 843, 912, 1482, 1879
MslI CAYNNNNRTG 2 cut(s) 1148, 1364
MspA1I CMGCKG 1 cut(s) 542
MspR9I CCNGG 3 cut(s) 95, 1661, 1760
MunI CAATTG 1 cut(s) 714
MvaI CCWGG 3 cut(s) 95, 1661, 1760
MwoI GCNNNNNNNGC 6 cut(s) 34, 563, 781, 1036, 1110, 1145
NcoI CCATGG 2 cut(s) 1184, 1785
NdeI CATATG 3 cut(s) 377, 403, 1645
NdeII GATC 5 cut(s) 145, 231, 337, 1575, 1640
NlaIII CATG 9 cut(s) 262, 531, 831, 925, 1117, 1188, 1273, 1789, 1813
NlaIV GGNNCC 5 cut(s) 269, 455, 578, 833, 1665
NmeAIII GCCGAG 1 cut(s) 1778
NmuCI GTSAC 1 cut(s) 1097
PagI TCATGA 1 cut(s) 1269
PceI AGGCCT 1 cut(s) 1412
PciSI GCTCTTC 1 cut(s) 529
PdmI GAANNNNTTC 1 cut(s) 1248
PfeI GAWTC 7 cut(s) 301, 371, 665, 719, 1517, 1634, 1792
PflMI CCANNNNNTGG 3 cut(s) 264, 1190, 1570
PkrI GCNGC 8 cut(s) 44, 223, 526, 541, 697, 1651, 1780, 1783
Ple19I CGATCG 1 cut(s) 148
PleI GAGTC 3 cut(s) 391, 412, 1188
PpsI GAGTC 3 cut(s) 391, 412, 1188
Psp124BI GAGCTC 1 cut(s) 39
Psp1406I AACGTT 1 cut(s) 177
Psp6I CCWGG 3 cut(s) 93, 1659, 1758
PspFI CCCAGC 1 cut(s) 343
PspGI CCWGG 3 cut(s) 93, 1659, 1758
PspN4I GGNNCC 5 cut(s) 269, 455, 578, 833, 1665
PspPI GGNCC 4 cut(s) 26, 97, 268, 831
PsrI GAACNNNNNNTAC 1 cut(s) 1858
PsuI RGATCY 1 cut(s) 337
PvuI CGATCG 1 cut(s) 148
PvuII CAGCTG 1 cut(s) 542
RsaI GTAC 5 cut(s) 410, 431, 455, 578, 1692
RsaNI GTAC 5 cut(s) 409, 430, 454, 577, 1691
RseI CAYNNNNRTG 2 cut(s) 1148, 1364
SacI GAGCTC 1 cut(s) 39
SapI GCTCTTC 1 cut(s) 529
SaqAI TTAA 6 cut(s) 116, 287, 843, 912, 1482, 1879
SatI GCNGC 8 cut(s) 43, 222, 525, 540, 696, 1650, 1779, 1782
Sau3AI GATC 5 cut(s) 145, 231, 337, 1575, 1640
Sau96I GGNCC 4 cut(s) 26, 97, 268, 831
SchI GAGTC 3 cut(s) 391, 412, 1189
ScrFI CCNGG 3 cut(s) 95, 1661, 1760
SduI GDGCHC 2 cut(s) 39, 896
SfaNI GCATC 2 cut(s) 140, 1356
SfcI CTRYAG 1 cut(s) 785
SinI GGWCC 3 cut(s) 97, 268, 831
SmiMI CAYNNNNRTG 2 cut(s) 1148, 1364
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SpeI ACTAGT 1 cut(s) 1045
SseBI AGGCCT 1 cut(s) 1412
SsiI CCGC 5 cut(s) 40, 43, 221, 224, 620
SspMI CTAG 6 cut(s) 23, 293, 1046, 1496, 1596, 1707
SstI GAGCTC 1 cut(s) 39
StuI AGGCCT 1 cut(s) 1412
StyD4I CCNGG 3 cut(s) 93, 1659, 1758
StyI CCWWGG 4 cut(s) 22, 1051, 1184, 1785
TaaI ACNGT 4 cut(s) 789, 1673, 1686, 1853
TaiI ACGT 1 cut(s) 180
TaqI TCGA 2 cut(s) 148, 1252
TaqII GACCGA 1 cut(s) 94
TauI GCSGC 2 cut(s) 45, 224
TfiI GAWTC 7 cut(s) 301, 371, 665, 719, 1517, 1634, 1792
Tru1I TTAA 6 cut(s) 116, 287, 843, 912, 1482, 1879
Tru9I TTAA 6 cut(s) 116, 287, 843, 912, 1482, 1879
TscAI CASTG 5 cut(s) 464, 804, 1040, 1216, 1557
TseFI GTSAC 1 cut(s) 1097
TseI GCWGC 6 cut(s) 524, 539, 695, 1649, 1778, 1781
Tsp45I GTSAC 1 cut(s) 1097
TspDTI ATGAA 4 cut(s) 429, 938, 1032, 1258
TspRI CASTG 5 cut(s) 464, 804, 1040, 1216, 1557
Van91I CCANNNNNTGG 3 cut(s) 264, 1190, 1570
VpaK11BI GGWCC 3 cut(s) 97, 268, 831
XapI RAATTY 6 cut(s) 348, 656, 701, 1224, 1507, 1821
XcmI CCANNNNNNNNNTGG 1 cut(s) 408
XmaJI CCTAGG 1 cut(s) 22
XmnI GAANNNNTTC 1 cut(s) 1248
XspI CTAG 6 cut(s) 23, 293, 1046, 1496, 1596, 1707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.