Rh6BG058600

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001292
Physical Location & Seq
Forward (+)
9256838 .. 9259370
2533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG058600.1

Sequence Viewer

Length: 1938 bp
ATGGCTCCTGCTTTATCTCTACTAGGGCCACCAGCTCTCCGCCAGCCTAAACCCACTTTTCACCATCCCTCCGCCCCTATCTCAAGATTCTCAACCTACCAACACCTTGCAGAGGGTCAATACCTCGAGTCTTCTCTATCACCCTTGGACCCCTGCACCAAGTTTCTCTTAGAAGCCATCACATACCCCAAGAGCCGTTCCGATTACAACTACGACTATGACTACGACGAGCAACCAAAGGATCTGGCTCACTATCAAGGAGAGGCGTGGAAGCATGACCCCCTCACCACTCTCAAGCTCATATGTAGTCTTCGAATGATGGGGAAGCTCAACGAAGATCGGTTTTACAAAGTCATGTTGTGGGTGCACAATAACCACCCTCTAACCATGTCTTTGAATGTCATGGCTTTTGGGGGTTTAGGTATGTTCAAGGATCTTTTGGGGGTTCTGTATAGGGTTTTGGAGGATTCCATAGAAAAACCAAACAACGAATACTCGCGGTGGAATTATGATTATTCTCGGGAAGAAGAGACGTCAACAAATAAGGAAGGTATTGATAATAATTCTAGGGCTAAAACCGCCGTTGAGAGGTACCAAAACGACTCAGATTATCGAAACTTCCATGACTGGATATCGGAGATGTTTGCAGAACTTCTGAGATCCGATCTCAGGTTTTTGGAGTCGGGTGAGGTTGAAAAAATCAGCTTTGCTTCCAAATTTTGTCCTTCAGTCAATTCTTCATATGATAAAGCCACGCTGATATGTGAAAATATAGCAAGGAGAATGTTCCCGAAGCATTGTTATGAAGAATACAAAGATGTTGGGGAAGCTCATTATGCCTACAGGGTTCGTGACCGCCTGCGGAAACAGGTACTCATTCCTCTGCGCAAAGCCTTAAAATTATCATCGGTGGAGATGAAGAATATGTTGGCTTTCGACAACAGCAAAGCCTTTAATGGTTTGAAAATATACGAGGAGATTTCTAACCATGGTTATCTAAAGGGAAGCAAATTACTATTTCAAAAGTGCATGAAGCTTGTGGAGATGTTTGGGAATCCAAGCATTGGTGACGGGCTGAAACTTCCTAACGATATTGTAGCTTCCTTGATGAGCAAGGAACATAGTGATGAAATTGTGGAGCGTCAATGGCGGAAACTAGTCCAAGAGTTGTCAAACCAAGGCAAGCTGAGGAATTGTCTTGCCGTGTGTGAACTTCCCTTGAACATGAGAGGGACATACAAGGAGACAGCTTGTATTTCACTGGGGTTATTGATTTCAGAGCTGAGCGAAAATCCATGGAAAGGAAAGGTTTTCTCATTCAATGAAACTCCAAGACTTCAAAAGATCGAAGGAGACGATCTTGAATCCAAGTGTGAGTTCATGAGACACAACATAGAGTGTGGAGACAAAGTTGATTTCTTCAAGATTTACAACCAAGTCTTGCAAATTGCCATTGAACAAAAGCTAAGCAACGAGCAGTTGCCCAAGAGGATTTTTGTGTTCACTTATAGGGATTTCGAGGCGGCTTCCAAGAACAATTGGGCAGAGGATTATAAAGAGGCATGGACTAATTACAGAAGAAGAGGGTACGCAAATGTACCACTGGTGGAGGCTAGGACTCAATACAAAAAGAAAGGGTACAAAACTCTGCCACACATTTTGTTTTGGAATCTTAAGAATTCCATTGCTGAGCCAGAGATAATTTGTAGCCCTGTTAATAACAAATGTGGGGGGCTGATTATAACTGGGTTGTCGAACAGTTTGATCAGTATGTTCTTAGTGGGAGAGTCGGATTCGAGAACATATGCACCTCATGTCCAGGCACCTTATGGTATCGGTTCGCTGTCGGTACTGAAATTTCTTCCCAAAGCTGAAGATGTGATGAAATCTGTATTTTTGAGAGATGAATTATTGAAGAACCTCCTTATATTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

645

Amino Acids

74.54

Weight (kDa)

7.82

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 73 - 396 7.7e-61 Domain of unknown function (DUF2828)
DUF7788 PF25043 398 - 534 3.3e-30 Domain of unknown function (DUF7788)
DUF7788 PF25043 540 - 596 3.2e-06 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1554, 1742
AatII GACGTC 1 cut(s) 536
Acc16I TGCGCA 1 cut(s) 887
Acc65I GGTACC 1 cut(s) 591
AccB1I GGYRCC 2 cut(s) 591, 1822
AccB7I CCANNNNNTGG 1 cut(s) 1064
AccII CGCG 1 cut(s) 499
AciI CCGC 8 cut(s) 40, 72, 499, 579, 856, 862, 1150, 1523
AclWI GGATC 3 cut(s) 249, 441, 654
AcsI RAATTY 3 cut(s) 716, 1678, 1856
AcuI CTGAAG 2 cut(s) 711, 1893
AcyI GRCGYC 1 cut(s) 533
AfaI GTAC 6 cut(s) 593, 873, 1589, 1599, 1640, 1851
AfiI CCNNNNNNNGG 6 cut(s) 112, 588, 669, 1064, 1301, 1930
AflII CTTAAG 1 cut(s) 1673
AhlI ACTAGT 1 cut(s) 1156
AjnI CCWGG 1 cut(s) 1818
AjuI GAANNNNNNNTTGG 4 cut(s) 911, 943, 1361, 1393
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 5 cut(s) 524, 1238, 1347, 1378, 1398
Alw44I GTGCAC 1 cut(s) 365
AlwI GGATC 3 cut(s) 249, 441, 654
Ama87I CYCGRG 2 cut(s) 125, 519
AoxI GGCC 1 cut(s) 26
ApaLI GTGCAC 1 cut(s) 365
ApoI RAATTY 3 cut(s) 716, 1678, 1856
Asp718I GGTACC 1 cut(s) 591
AspLEI GCGC 1 cut(s) 888
AspS9I GGNCC 2 cut(s) 26, 148
AsuHPI GGTGA 5 cut(s) 53, 132, 277, 698, 1079
AsuII TTCGAA 1 cut(s) 313
AvaI CYCGRG 2 cut(s) 125, 519
AvaII GGWCC 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 369
BanI GGYRCC 2 cut(s) 591, 1822
BbsI GAAGAC 2 cut(s) 123, 302
Bbv12I GWGCWC 1 cut(s) 369
BbvCI CCTCAGC 1 cut(s) 1187
BccI CCATC 3 cut(s) 72, 185, 313
BceAI ACGGC 3 cut(s) 180, 566, 1187
BciT130I CCWGG 1 cut(s) 1820
BclI TGATCA 1 cut(s) 1764
BcoDI GTCTC 5 cut(s) 524, 1238, 1347, 1378, 1398
BcuI ACTAGT 1 cut(s) 1156
BfaI CTAG 4 cut(s) 23, 567, 1157, 1614
BfmI CTRYAG 1 cut(s) 841
BfrI CTTAAG 1 cut(s) 1673
BisI GCNGC 1 cut(s) 1524
BlpI GCTNAGC 3 cut(s) 1283, 1466, 1689
BlsI GCNGC 1 cut(s) 1525
Bme1390I CCNGG 1 cut(s) 1820
Bme18I GGWCC 1 cut(s) 148
BmeT110I CYCGRG 2 cut(s) 125, 519
BmgT120I GGNCC 2 cut(s) 26, 148
BmiI GGNNCC 4 cut(s) 6, 150, 593, 1824
BmrFI CCNGG 1 cut(s) 1820
BmrI ACTGGG 2 cut(s) 1271, 1755
BmuI ACTGGG 2 cut(s) 1271, 1755
BpiI GAAGAC 2 cut(s) 123, 302
Bpu10I CCTNAGC 1 cut(s) 1187
Bpu1102I GCTNAGC 3 cut(s) 1283, 1466, 1689
Bpu14I TTCGAA 1 cut(s) 313
BpuEI CTTGAG 2 cut(s) 67, 278
BsaHI GRCGYC 1 cut(s) 533
BsaJI CCNNGG 4 cut(s) 144, 988, 1177, 1295
BsaXI ACNNNNNCTCC 6 cut(s) 21, 51, 53, 83, 1395, 1425
Bsc4I CCNNNNNNNGG 6 cut(s) 112, 588, 669, 1064, 1301, 1930
Bse1I ACTGG 4 cut(s) 632, 1266, 1608, 1750
Bse3DI GCAATG 1 cut(s) 1683
BseBI CCWGG 1 cut(s) 1820
BseDI CCNNGG 4 cut(s) 144, 988, 1177, 1295
BseGI GGATG 1 cut(s) 64
BseLI CCNNNNNNNGG 6 cut(s) 112, 588, 669, 1064, 1301, 1930
BseMI GCAATG 1 cut(s) 1683
BseMII CTCAG 6 cut(s) 618, 647, 682, 1178, 1274, 1680
BseNI ACTGG 4 cut(s) 632, 1266, 1608, 1750
BseRI GAGGAG 1 cut(s) 989
BseSI GKGCMC 1 cut(s) 369
BsgI GTGCAG 1 cut(s) 139
Bsh1236I CGCG 1 cut(s) 499
BshFI GGCC 1 cut(s) 28
BshNI GGYRCC 2 cut(s) 591, 1822
BsiHKAI GWGCWC 1 cut(s) 369
BsiHKCI CYCGRG 2 cut(s) 125, 519
BslFI GGGAC 1 cut(s) 1246
BslI CCNNNNNNNGG 6 cut(s) 112, 588, 669, 1064, 1301, 1930
BsmAI GTCTC 5 cut(s) 524, 1238, 1347, 1378, 1398
BsmBI CGTCTC 2 cut(s) 524, 1347
BsmFI GGGAC 1 cut(s) 1246
BsnI GGCC 1 cut(s) 28
BsoBI CYCGRG 2 cut(s) 125, 519
Bsp119I TTCGAA 1 cut(s) 313
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 8 cut(s) 241, 337, 433, 659, 664, 1344, 1357, 1764
Bsp1720I GCTNAGC 3 cut(s) 1283, 1466, 1689
Bsp19I CCATGG 2 cut(s) 988, 1295
BspACI CCGC 8 cut(s) 40, 72, 499, 579, 856, 862, 1150, 1523
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 6 cut(s) 617, 648, 681, 1179, 1275, 1681
BspFNI CGCG 1 cut(s) 499
BspHI TCATGA 1 cut(s) 1380
BspLI GGNNCC 4 cut(s) 6, 150, 593, 1824
BspPI GGATC 3 cut(s) 249, 441, 654
BspT104I TTCGAA 1 cut(s) 313
BspT107I GGYRCC 2 cut(s) 591, 1822
BspTI CTTAAG 1 cut(s) 1673
BsrDI GCAATG 1 cut(s) 1683
BsrI ACTGG 4 cut(s) 632, 1266, 1608, 1750
BssECI CCNNGG 4 cut(s) 144, 988, 1177, 1295
BssMI GATC 8 cut(s) 241, 337, 433, 659, 664, 1344, 1357, 1764
BssNI GRCGYC 1 cut(s) 533
BssT1I CCWWGG 4 cut(s) 144, 988, 1177, 1295
Bst2UI CCWGG 1 cut(s) 1820
Bst4CI ACNGT 1 cut(s) 1760
Bst6I CTCTTC 2 cut(s) 522, 1576
BstACI GRCGYC 1 cut(s) 533
BstAFI CTTAAG 1 cut(s) 1673
BstBI TTCGAA 1 cut(s) 313
BstC8I GCNNGC 3 cut(s) 44, 860, 1184
BstDEI CTNAG 9 cut(s) 169, 604, 656, 668, 1187, 1283, 1466, 1689, 1777
BstDSI CCRYGG 2 cut(s) 988, 1295
BstENI CCTNNNNNAGG 1 cut(s) 110
BstF5I GGATG 1 cut(s) 64
BstFNI CGCG 1 cut(s) 499
BstHHI GCGC 1 cut(s) 888
BstKTI GATC 8 cut(s) 244, 340, 436, 662, 667, 1347, 1360, 1767
BstMAI GTCTC 5 cut(s) 524, 1238, 1347, 1378, 1398
BstMBI GATC 8 cut(s) 241, 337, 433, 659, 664, 1344, 1357, 1764
BstMWI GCNNNNNNNGC 3 cut(s) 578, 836, 1147
BstNI CCWGG 1 cut(s) 1820
BstSCI CCNGG 1 cut(s) 1818
BstSFI CTRYAG 1 cut(s) 841
BstSLI GKGCMC 1 cut(s) 369
BstUI CGCG 1 cut(s) 499
BstV2I GAAGAC 2 cut(s) 123, 302
BstX2I RGATCY 3 cut(s) 241, 433, 659
BstYI RGATCY 3 cut(s) 241, 433, 659
BsuRI GGCC 1 cut(s) 28
BtgI CCRYGG 2 cut(s) 988, 1295
BtsCI GGATG 1 cut(s) 64
BtsIMutI CAGTG 2 cut(s) 1259, 1601
Cac8I GCNNGC 3 cut(s) 44, 860, 1184
CciI TCATGA 1 cut(s) 1380
CfoI GCGC 1 cut(s) 888
Cfr13I GGNCC 2 cut(s) 26, 148
CseI GACGC 1 cut(s) 1130
Csp6I GTAC 6 cut(s) 592, 872, 1588, 1598, 1639, 1850
CspCI CAANNNNNGTGG 2 cut(s) 1641, 1676
CviQI GTAC 6 cut(s) 592, 872, 1588, 1598, 1639, 1850
DdeI CTNAG 9 cut(s) 169, 604, 656, 668, 1187, 1283, 1466, 1689, 1777
DpnI GATC 8 cut(s) 243, 339, 435, 661, 666, 1346, 1359, 1766
DpnII GATC 8 cut(s) 241, 337, 433, 659, 664, 1344, 1357, 1764
Eam1104I CTCTTC 2 cut(s) 522, 1576
EarI CTCTTC 2 cut(s) 522, 1576
EciI GGCGGA 3 cut(s) 29, 61, 1165
Eco130I CCWWGG 4 cut(s) 144, 988, 1177, 1295
Eco32I GATATC 1 cut(s) 633
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 2 cut(s) 711, 1893
Eco88I CYCGRG 2 cut(s) 125, 519
EcoNI CCTNNNNNAGG 1 cut(s) 110
EcoRI GAATTC 1 cut(s) 1678
EcoRII CCWGG 1 cut(s) 1818
EcoRV GATATC 1 cut(s) 633
EcoT14I CCWWGG 4 cut(s) 144, 988, 1177, 1295
ErhI CCWWGG 4 cut(s) 144, 988, 1177, 1295
Esp3I CGTCTC 2 cut(s) 524, 1347
FalI AAGNNNNNCTT 2 cut(s) 152, 184
FaqI GGGAC 1 cut(s) 1246
FauNDI CATATG 3 cut(s) 302, 742, 1804
FbaI TGATCA 1 cut(s) 1764
Fnu4HI GCNGC 1 cut(s) 1524
FokI GGATG 1 cut(s) 51
Fsp4HI GCNGC 1 cut(s) 1524
FspBI CTAG 4 cut(s) 23, 567, 1157, 1614
FspI TGCGCA 1 cut(s) 887
GlaI GCGC 1 cut(s) 887
GluI GCNGC 1 cut(s) 1524
HaeIII GGCC 1 cut(s) 28
HgaI GACGC 1 cut(s) 1130
HhaI GCGC 1 cut(s) 888
Hin1I GRCGYC 1 cut(s) 533
Hin6I GCGC 1 cut(s) 886
HinP1I GCGC 1 cut(s) 886
HincII GTYRAC 1 cut(s) 537
HindII GTYRAC 1 cut(s) 537
HindIII AAGCTT 1 cut(s) 1034
HphI GGTGA 5 cut(s) 53, 132, 277, 698, 1079
Hpy166II GTNNAC 4 cut(s) 367, 537, 1211, 1503
Hpy188I TCNGA 7 cut(s) 202, 607, 637, 657, 664, 1279, 1792
Hpy188III TCNNGA 8 cut(s) 84, 521, 790, 851, 1361, 1381, 1423, 1797
Hpy8I GTNNAC 4 cut(s) 367, 537, 1211, 1503
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 3 cut(s) 542, 735, 1343
HpyCH4III ACNGT 1 cut(s) 1760
HpyCH4IV ACGT 1 cut(s) 533
HpyCH4V TGCA 7 cut(s) 110, 156, 367, 647, 1029, 1444, 1808
HpyF10VI GCNNNNNNNGC 3 cut(s) 578, 836, 1147
HpyF3I CTNAG 9 cut(s) 169, 604, 656, 668, 1187, 1283, 1466, 1689, 1777
HpySE526I ACGT 1 cut(s) 533
Hsp92I GRCGYC 1 cut(s) 533
HspAI GCGC 1 cut(s) 886
KpnI GGTACC 1 cut(s) 595
Ksp22I TGATCA 1 cut(s) 1764
Kzo9I GATC 8 cut(s) 241, 337, 433, 659, 664, 1344, 1357, 1764
LmnI GCTCC 2 cut(s) 10, 1138
MaeI CTAG 4 cut(s) 23, 567, 1157, 1614
MaeII ACGT 1 cut(s) 533
MaeIII GTNAC 2 cut(s) 851, 1067
MalI GATC 8 cut(s) 243, 339, 435, 661, 666, 1346, 1359, 1766
MboI GATC 8 cut(s) 241, 337, 433, 659, 664, 1344, 1357, 1764
MfeI CAATTG 1 cut(s) 1537
MflI RGATCY 3 cut(s) 241, 433, 659
MhlI GDGCHC 1 cut(s) 369
MlyI GAGTC 5 cut(s) 137, 596, 689, 1612, 1796
MmeI TCCRAC 1 cut(s) 1770
MseI TTAA 4 cut(s) 896, 954, 1674, 1716
MslI CAYNNNNRTG 2 cut(s) 801, 1125
MspCI CTTAAG 1 cut(s) 1673
MspR9I CCNGG 1 cut(s) 1820
MunI CAATTG 1 cut(s) 1537
MvaI CCWGG 1 cut(s) 1820
MvnI CGCG 1 cut(s) 499
MwoI GCNNNNNNNGC 3 cut(s) 578, 836, 1147
NcoI CCATGG 2 cut(s) 988, 1295
NdeI CATATG 3 cut(s) 302, 742, 1804
NdeII GATC 8 cut(s) 241, 337, 433, 659, 664, 1344, 1357, 1764
NlaIV GGNNCC 4 cut(s) 6, 150, 593, 1824
NmuCI GTSAC 2 cut(s) 851, 1067
NsbI TGCGCA 1 cut(s) 887
NspV TTCGAA 1 cut(s) 313
PaeR7I CTCGAG 1 cut(s) 125
PagI TCATGA 1 cut(s) 1380
PcsI WCGNNNNNNNCGW 1 cut(s) 1353
PfeI GAWTC 6 cut(s) 87, 467, 1054, 1364, 1669, 1793
PflMI CCANNNNNTGG 1 cut(s) 1064
PkrI GCNGC 1 cut(s) 1525
PleI GAGTC 5 cut(s) 136, 596, 688, 1612, 1795
PpsI GAGTC 5 cut(s) 136, 596, 688, 1612, 1795
PsiI TTATAA 2 cut(s) 1554, 1742
Psp6I CCWGG 1 cut(s) 1818
PspGI CCWGG 1 cut(s) 1818
PspN4I GGNNCC 4 cut(s) 6, 150, 593, 1824
PspPI GGNCC 2 cut(s) 26, 148
PspXI VCTCGAGB 1 cut(s) 125
PsuI RGATCY 3 cut(s) 241, 433, 659
RsaI GTAC 6 cut(s) 593, 873, 1589, 1599, 1640, 1851
RsaNI GTAC 6 cut(s) 592, 872, 1588, 1598, 1639, 1850
RseI CAYNNNNRTG 2 cut(s) 801, 1125
SaqAI TTAA 4 cut(s) 896, 954, 1674, 1716
SatI GCNGC 1 cut(s) 1524
Sau3AI GATC 8 cut(s) 241, 337, 433, 659, 664, 1344, 1357, 1764
Sau96I GGNCC 2 cut(s) 26, 148
SchI GAGTC 5 cut(s) 137, 596, 689, 1612, 1796
ScrFI CCNGG 1 cut(s) 1820
SduI GDGCHC 1 cut(s) 369
SfcI CTRYAG 1 cut(s) 841
Sfr274I CTCGAG 1 cut(s) 125
SfuI TTCGAA 1 cut(s) 313
SinI GGWCC 1 cut(s) 148
SlaI CTCGAG 1 cut(s) 125
SmiMI CAYNNNNRTG 2 cut(s) 801, 1125
SmlI CTYRAG 4 cut(s) 82, 125, 293, 1673
SmoI CTYRAG 4 cut(s) 82, 125, 293, 1673
SpeI ACTAGT 1 cut(s) 1156
SsiI CCGC 8 cut(s) 40, 72, 499, 579, 856, 862, 1150, 1523
SspMI CTAG 4 cut(s) 23, 567, 1157, 1614
StyD4I CCNGG 1 cut(s) 1818
StyI CCWWGG 4 cut(s) 144, 988, 1177, 1295
TaaI ACNGT 1 cut(s) 1760
TaiI ACGT 1 cut(s) 536
TaqI TCGA 8 cut(s) 126, 313, 613, 936, 1347, 1518, 1754, 1796
TauI GCSGC 1 cut(s) 1526
TfiI GAWTC 6 cut(s) 87, 467, 1054, 1364, 1669, 1793
Tru1I TTAA 4 cut(s) 896, 954, 1674, 1716
Tru9I TTAA 4 cut(s) 896, 954, 1674, 1716
TscAI CASTG 2 cut(s) 1266, 1608
TseFI GTSAC 2 cut(s) 851, 1067
Tsp45I GTSAC 2 cut(s) 851, 1067
TspDTI ATGAA 9 cut(s) 729, 819, 932, 1046, 1143, 1338, 1369, 1898, 1920
TspRI CASTG 2 cut(s) 1266, 1608
Van91I CCANNNNNTGG 1 cut(s) 1064
Vha464I CTTAAG 1 cut(s) 1673
VneI GTGCAC 1 cut(s) 365
VpaK11BI GGWCC 1 cut(s) 148
XagI CCTNNNNNAGG 1 cut(s) 110
XapI RAATTY 3 cut(s) 716, 1678, 1856
XcmI CCANNNNNNNNNTGG 1 cut(s) 1826
XhoI CTCGAG 1 cut(s) 125
XspI CTAG 4 cut(s) 23, 567, 1157, 1614
ZraI GACGTC 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.