Prupe.8G140200_v2.0.a1

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001292
Physical Location & Seq
Reverse (-)
15866344 .. 15868263
1920 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G140200.1

Sequence Viewer

Length: 1920 bp
ATGGGTAGAGGCCAATCTAGGCAGTCTTCACACCCAACCTCAAACCCGTGCATTACATTTTTCTTCAAAGCCAGCCGATACACTGCTGATCACACCCGGAAAAAGACCCCTCCCGAGTACTGGTACTTCGGGGGGATCCAGCGGGATCTGATTTATTATGAAGAAGAGGCATGGAAGTTTGACCCTCTCTCCACTTTCAAGCTCATATTTGCTCTTCGAATGGTGGAGAGGCTTGACTTGTTGTGGGTGCACAAAAACCACCCTTTAACCCTGTCGCTGAATGTCATGGCTTTGGGGGGTAAAGGTTGGTTCAAAGATCTATTGGGGTTTCTCTATTTTGTTTTGGAAGACCCGATGAAGGAAGCAGAAGCAAAAGCAAAATCAAAGAAGAATGGAATAATCAATTATGACTATGCATATTATGATTCTGACGAAGATGACTTTTATCAGAGTTATCCGTACAAGGAAGAAACTGGGGTTGAGGAGAAGAACAGAGAAGAGATAACTGGCACAGATACTCGCATTGGCAGGGCTAAAATGGCGGTCGAGAGGTACCAAAGTGACCCGGATTATCGAAACTTGCATGATCGAATCTCGGAGATGTTTGCACATTTTTTGACATCGGATCTCAGGTTTCTGGAGTCTGGTGAGATCGAAAAAATCAGTTTTGCTTACAAATTTTGTGCTTCAGTTAATTCTGGTTATGACAGAGCAACCCTGCTGTGTGAAAACATAGCAAAGAGAATTTTCCCACGCCATGACTATGAAGAGTACAGTGAGCTTGAAGAAGCTCATTACGCTTACAGGGTTCGTGATCGTTTGAGGAAACAAGTACTTGTTCCTCTGCGCAAAGTGCTAGAATCATCATCATCATCAAGGAAGATCAGATATGTTCCTACTGTACCAGGGCTATTATCCCAAGACCCTAAGGCCCTGGCTGGTTTGAGAAGATACAGGAGGATCCTGAGCAATGAGAGAGAATATGAAAGCACATTGTATTTGATGGAAAATGGAGGCGAAAATGGAAGCAAATCTCAATTTAAGCTTTATATAAACGTTGTTGAATTTTTCCGGGTAAGCATTGGTGATGGGCTAAGACTTCCACACCAAATAGTATTTCCTTTCTTGTACATGAACAAGGAACATAATGAAAGATCAGAGCTTGAATGGCAGACACTAGTCCAAGATTTTTCAAACAAAGGGAAGCTGAGGAATTGTCTTGCCGTATGTGACATCCAAGAGAGCATGAGAAAGACATACGAGGATATGGTTTGCATTGGCATGGGGTTATTGATTTCAGAACTGAGTGAAAATCCATGGAATGGAATGGTTTTTCCCTTTAGCCTTTCTCCCAAGCTTTGCAAGATCGAAGGAGAAAATCTTCAGTCCAAGTGCGAGTTCATGAGGCGGATAAATCTTCAGTCCAAGGGGATAGAGTGTTCTGTGAAATTGGACTTCTTAGCAGTTTACAACCAAATCTTGGCCACTGCAACCTCACAAAATCTAAGCCCTGAAAAGATGCCCAAGAGGATTTTTGTGTTCACCAACACGGATTTTCGGAAAGCCTTCAAATGCGATTGGCGGGACAATTATAGAAAGGCTTTGAAGAATTACAGAAGAAGAGGGTATCAGACTCTGCAAGATATGGTTTTTTGGAATCTTAATGGTGGCATGCGTGAGCCAGAGGTTATTAATTGCCCATTGAAGGAGAACCATAAAGTAGGTCTTATTTTAACTGGGTTTTCAAACAATATGCTCACTATGTTCTTGAATGGAGAGTCAGATTCAAGACCATACGCAGCTCATCAGATCCAAGCACCTTATGGTATTGATGTACAGAAATGCATTCCTAGAGCAGAGGATGTGATGAAATGTGCAATTTCAGGACCAGAATTCGATAACCTTCTTGTATTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

640

Amino Acids

74.63

Weight (kDa)

8.22

Isoelectric Point (pI)

47.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 848
Acc65I GGTACC 1 cut(s) 552
AccB1I GGYRCC 1 cut(s) 552
AccB7I CCANNNNNTGG 2 cut(s) 1322, 1480
AciI CCGC 4 cut(s) 142, 542, 1408, 1582
AclI AACGTT 1 cut(s) 1056
AclWI GGATC 7 cut(s) 130, 143, 153, 633, 955, 968, 1804
AcoI YGGCCR 1 cut(s) 1482
AcsI RAATTY 4 cut(s) 677, 744, 1064, 1892
AcuI CTGAAG 3 cut(s) 672, 1367, 1403
AfaI GTAC 9 cut(s) 119, 125, 461, 554, 773, 834, 903, 1130, 1836
AfiI CCNNNNNNNGG 5 cut(s) 120, 358, 1322, 1480, 1705
AhlI ACTAGT 1 cut(s) 1177
AjnI CCWGG 2 cut(s) 904, 933
AjuI GAANNNNNNNTTGG 4 cut(s) 1101, 1133, 1382, 1414
AloI GAACNNNNNNTCC 2 cut(s) 1423, 1455
AluBI AGCT 8 cut(s) 202, 781, 791, 1045, 1162, 1207, 1357, 1802
AluI AGCT 8 cut(s) 202, 781, 791, 1045, 1162, 1207, 1357, 1802
Alw21I GWGCWC 1 cut(s) 252
Alw44I GTGCAC 1 cut(s) 248
AlwI GGATC 7 cut(s) 130, 143, 153, 633, 955, 968, 1804
AlwNI CAGNNNCTG 1 cut(s) 1636
Ama87I CYCGRG 1 cut(s) 113
AoxI GGCC 3 cut(s) 10, 930, 1482
ApaLI GTGCAC 1 cut(s) 248
ApeKI GCWGC 1 cut(s) 1799
ApoI RAATTY 4 cut(s) 677, 744, 1064, 1892
AseI ATTAAT 1 cut(s) 1692
Asp700I GAANNNNTTC 2 cut(s) 1380, 1565
Asp718I GGTACC 1 cut(s) 552
AspLEI GCGC 1 cut(s) 849
AspS9I GGNCC 2 cut(s) 931, 1886
AsuC2I CCSGG 3 cut(s) 97, 566, 1073
AsuHPI GGTGA 3 cut(s) 659, 1097, 1534
AsuII TTCGAA 1 cut(s) 217
AvaI CYCGRG 1 cut(s) 113
AvaII GGWCC 1 cut(s) 1886
AxyI CCTNAGG 1 cut(s) 927
BaeGI GKGCMC 1 cut(s) 252
BaeI ACNNNNGTAYC 2 cut(s) 544, 577
BalI TGGCCA 1 cut(s) 1484
BamHI GGATCC 2 cut(s) 135, 960
BanI GGYRCC 1 cut(s) 552
BarI GAAGNNNNNNTAC 2 cut(s) 110, 142
BbsI GAAGAC 2 cut(s) 18, 354
Bbv12I GWGCWC 1 cut(s) 252
BbvCI CCTCAGC 1 cut(s) 1208
BbvI GCAGC 1 cut(s) 1811
BccI CCATC 2 cut(s) 997, 1082
BceAI ACGGC 1 cut(s) 1208
BciT130I CCWGG 2 cut(s) 906, 935
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 3 cut(s) 97, 566, 1073
BcuI ACTAGT 1 cut(s) 1177
BfaI CTAG 4 cut(s) 18, 857, 1178, 1851
BglII AGATCT 1 cut(s) 316
BisI GCNGC 1 cut(s) 1800
BlsI GCNGC 1 cut(s) 1801
BmcAI AGTACT 2 cut(s) 119, 834
Bme1390I CCNGG 5 cut(s) 97, 566, 906, 935, 1073
Bme18I GGWCC 1 cut(s) 1886
BmeT110I CYCGRG 1 cut(s) 113
BmgT120I GGNCC 2 cut(s) 931, 1886
BmiI GGNNCC 3 cut(s) 137, 554, 962
BmrFI CCNGG 5 cut(s) 97, 566, 906, 935, 1073
BmrI ACTGGG 2 cut(s) 483, 1746
BmsI GCATC 1 cut(s) 1509
BmuI ACTGGG 2 cut(s) 483, 1746
BoxI GACNNNNGTC 1 cut(s) 1178
BpiI GAAGAC 2 cut(s) 18, 354
BpmI CTGGAG 1 cut(s) 659
Bpu10I CCTNAGC 2 cut(s) 965, 1208
Bpu14I TTCGAA 1 cut(s) 217
BpuMI CCSGG 3 cut(s) 97, 566, 1073
BsaJI CCNNGG 4 cut(s) 905, 933, 1316, 1425
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 5 cut(s) 120, 358, 1322, 1480, 1705
Bse1I ACTGG 4 cut(s) 125, 478, 511, 1741
Bse21I CCTNAGG 1 cut(s) 927
Bse3DI GCAATG 1 cut(s) 976
BseBI CCWGG 2 cut(s) 906, 935
BseDI CCNNGG 4 cut(s) 905, 933, 1316, 1425
BseGI GGATG 2 cut(s) 1233, 1867
BseLI CCNNNNNNNGG 5 cut(s) 120, 358, 1322, 1480, 1705
BseMI GCAATG 1 cut(s) 976
BseMII CTCAG 4 cut(s) 643, 956, 1199, 1295
BseNI ACTGG 4 cut(s) 125, 478, 511, 1741
BseRI GAGGAG 1 cut(s) 497
BseSI GKGCMC 1 cut(s) 252
BseXI GCAGC 1 cut(s) 1811
Bsh1285I CGRYCG 1 cut(s) 546
BshFI GGCC 3 cut(s) 12, 932, 1484
BshNI GGYRCC 1 cut(s) 552
BsiEI CGRYCG 1 cut(s) 546
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 3 cut(s) 97, 566, 1072
BslFI GGGAC 1 cut(s) 1598
BslI CCNNNNNNNGG 5 cut(s) 120, 358, 1322, 1480, 1705
BsmFI GGGAC 1 cut(s) 1598
BsmI GAATGC 1 cut(s) 1845
BsnI GGCC 3 cut(s) 12, 932, 1484
BsoBI CYCGRG 1 cut(s) 113
Bsp119I TTCGAA 1 cut(s) 217
Bsp1286I GDGCHC 1 cut(s) 252
Bsp1407I TGTACA 2 cut(s) 1128, 1834
Bsp19I CCATGG 1 cut(s) 1316
BspACI CCGC 4 cut(s) 142, 542, 1408, 1582
BspANI GGCC 3 cut(s) 12, 932, 1484
BspCNI CTCAG 4 cut(s) 642, 957, 1200, 1296
BspHI TCATGA 1 cut(s) 1401
BspLI GGNNCC 3 cut(s) 137, 554, 962
BspPI GGATC 7 cut(s) 130, 143, 153, 633, 955, 968, 1804
BspQI GCTCTTC 1 cut(s) 219
BspT104I TTCGAA 1 cut(s) 217
BspT107I GGYRCC 1 cut(s) 552
BsrDI GCAATG 1 cut(s) 976
BsrGI TGTACA 2 cut(s) 1128, 1834
BsrI ACTGG 4 cut(s) 125, 478, 511, 1741
BssECI CCNNGG 4 cut(s) 905, 933, 1316, 1425
BssT1I CCWWGG 2 cut(s) 1316, 1425
Bst2UI CCWGG 2 cut(s) 906, 935
Bst4CI ACNGT 2 cut(s) 776, 901
Bst6I CTCTTC 5 cut(s) 159, 219, 492, 762, 1615
BstAUI TGTACA 2 cut(s) 1128, 1834
BstBI TTCGAA 1 cut(s) 217
BstC8I GCNNGC 2 cut(s) 73, 1673
BstDEI CTNAG 8 cut(s) 629, 927, 965, 1094, 1208, 1304, 1459, 1505
BstDSI CCRYGG 1 cut(s) 1316
BstF5I GGATG 2 cut(s) 1233, 1867
BstHHI GCGC 1 cut(s) 849
BstMCI CGRYCG 1 cut(s) 546
BstMWI GCNNNNNNNGC 4 cut(s) 539, 797, 853, 1168
BstNI CCWGG 2 cut(s) 906, 935
BstNSI RCATGY 1 cut(s) 1675
BstPAI GACNNNNGTC 1 cut(s) 1178
BstSCI CCNGG 5 cut(s) 95, 564, 904, 933, 1071
BstSLI GKGCMC 1 cut(s) 252
BstV1I GCAGC 1 cut(s) 1811
BstV2I GAAGAC 2 cut(s) 18, 354
BstX2I RGATCY 6 cut(s) 135, 145, 316, 625, 960, 1809
BstYI RGATCY 6 cut(s) 135, 145, 316, 625, 960, 1809
Bsu36I CCTNAGG 1 cut(s) 927
BsuRI GGCC 3 cut(s) 12, 932, 1484
BtgI CCRYGG 1 cut(s) 1316
BtsCI GGATG 2 cut(s) 1233, 1867
BtsI GCAGTG 2 cut(s) 81, 1485
BtsIMutI CAGTG 3 cut(s) 81, 781, 1485
Cac8I GCNNGC 2 cut(s) 73, 1673
CaiI CAGNNNCTG 1 cut(s) 1636
CciI TCATGA 1 cut(s) 1401
CfoI GCGC 1 cut(s) 849
Cfr13I GGNCC 2 cut(s) 931, 1886
Csp6I GTAC 9 cut(s) 118, 124, 460, 553, 772, 833, 902, 1129, 1835
CviQI GTAC 9 cut(s) 118, 124, 460, 553, 772, 833, 902, 1129, 1835
DdeI CTNAG 8 cut(s) 629, 927, 965, 1094, 1208, 1304, 1459, 1505
EaeI YGGCCR 1 cut(s) 1482
Eam1104I CTCTTC 5 cut(s) 159, 219, 492, 762, 1615
EarI CTCTTC 5 cut(s) 159, 219, 492, 762, 1615
EciI GGCGGA 1 cut(s) 1423
Eco130I CCWWGG 2 cut(s) 1316, 1425
Eco47I GGWCC 1 cut(s) 1886
Eco57I CTGAAG 3 cut(s) 672, 1367, 1403
Eco81I CCTNAGG 1 cut(s) 927
Eco88I CYCGRG 1 cut(s) 113
EcoO109I RGGNCCY 1 cut(s) 931
EcoRI GAATTC 1 cut(s) 1892
EcoRII CCWGG 2 cut(s) 904, 933
EcoT14I CCWWGG 2 cut(s) 1316, 1425
EcoT22I ATGCAT 2 cut(s) 418, 1847
ErhI CCWWGG 2 cut(s) 1316, 1425
FalI AAGNNNNNCTT 2 cut(s) 1710, 1742
FaqI GGGAC 1 cut(s) 1598
FauI CCCGC 2 cut(s) 135, 1575
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 1 cut(s) 1800
FokI GGATG 2 cut(s) 1220, 1874
Fsp4HI GCNGC 1 cut(s) 1800
FspBI CTAG 4 cut(s) 18, 857, 1178, 1851
FspI TGCGCA 1 cut(s) 848
GlaI GCGC 1 cut(s) 848
GluI GCNGC 1 cut(s) 1800
GsuI CTGGAG 1 cut(s) 659
HaeIII GGCC 3 cut(s) 12, 932, 1484
HapII CCGG 3 cut(s) 97, 566, 1072
HhaI GCGC 1 cut(s) 849
Hin6I GCGC 1 cut(s) 847
HinP1I GCGC 1 cut(s) 847
HindIII AAGCTT 2 cut(s) 1043, 1355
HinfI GANTC 8 cut(s) 425, 591, 641, 860, 1633, 1657, 1778, 1784
HpaII CCGG 3 cut(s) 97, 566, 1072
HphI GGTGA 3 cut(s) 659, 1097, 1534
Hpy166II GTNNAC 3 cut(s) 250, 1468, 1542
Hpy188III TCNNGA 9 cut(s) 113, 547, 638, 812, 964, 1402, 1768, 1788, 1884
Hpy8I GTNNAC 3 cut(s) 250, 1468, 1542
HpyAV CCTTC 5 cut(s) 352, 1364, 1576, 1699, 1913
HpyCH4III ACNGT 2 cut(s) 776, 901
HpyCH4IV ACGT 1 cut(s) 1056
HpyF10VI GCNNNNNNNGC 4 cut(s) 539, 797, 853, 1168
HpyF3I CTNAG 8 cut(s) 629, 927, 965, 1094, 1208, 1304, 1459, 1505
HpySE526I ACGT 1 cut(s) 1056
HspAI GCGC 1 cut(s) 847
KpnI GGTACC 1 cut(s) 556
Ksp22I TGATCA 1 cut(s) 88
LguI GCTCTTC 1 cut(s) 219
Lsp1109I GCAGC 1 cut(s) 1811
LweI GCATC 1 cut(s) 1509
MaeI CTAG 4 cut(s) 18, 857, 1178, 1851
MaeII ACGT 1 cut(s) 1056
MaeIII GTNAC 2 cut(s) 560, 1229
MflI RGATCY 6 cut(s) 135, 145, 316, 625, 960, 1809
MhlI GDGCHC 1 cut(s) 252
MlsI TGGCCA 1 cut(s) 1484
MluNI TGGCCA 1 cut(s) 1484
MlyI GAGTC 3 cut(s) 650, 1627, 1787
Mox20I TGGCCA 1 cut(s) 1484
Mph1103I ATGCAT 2 cut(s) 418, 1847
MroXI GAANNNNTTC 2 cut(s) 1380, 1565
MscI TGGCCA 1 cut(s) 1484
MseI TTAA 6 cut(s) 266, 693, 1041, 1662, 1692, 1733
MslI CAYNNNNRTG 2 cut(s) 762, 1280
Msp20I TGGCCA 1 cut(s) 1484
MspA1I CMGCKG 1 cut(s) 142
MspI CCGG 3 cut(s) 97, 566, 1072
MspR9I CCNGG 5 cut(s) 97, 566, 906, 935, 1073
Mva1269I GAATGC 1 cut(s) 1845
MvaI CCWGG 2 cut(s) 906, 935
MwoI GCNNNNNNNGC 4 cut(s) 539, 797, 853, 1168
NciI CCSGG 3 cut(s) 97, 566, 1073
NcoI CCATGG 1 cut(s) 1316
NlaIV GGNNCC 3 cut(s) 137, 554, 962
NmuCI GTSAC 2 cut(s) 560, 1229
NsbI TGCGCA 1 cut(s) 848
NsiI ATGCAT 2 cut(s) 418, 1847
NspI RCATGY 1 cut(s) 1675
NspV TTCGAA 1 cut(s) 217
PaeI GCATGC 1 cut(s) 1675
PagI TCATGA 1 cut(s) 1401
PciSI GCTCTTC 1 cut(s) 219
PctI GAATGC 1 cut(s) 1845
PdmI GAANNNNTTC 2 cut(s) 1380, 1565
PfeI GAWTC 5 cut(s) 425, 591, 860, 1657, 1784
PflMI CCANNNNNTGG 2 cut(s) 1322, 1480
PkrI GCNGC 1 cut(s) 1801
PleI GAGTC 3 cut(s) 649, 1627, 1786
PpsI GAGTC 3 cut(s) 649, 1627, 1786
PshAI GACNNNNGTC 1 cut(s) 1178
PshBI ATTAAT 1 cut(s) 1692
Psp1406I AACGTT 1 cut(s) 1056
Psp6I CCWGG 2 cut(s) 904, 933
PspGI CCWGG 2 cut(s) 904, 933
PspN4I GGNNCC 3 cut(s) 137, 554, 962
PspPI GGNCC 2 cut(s) 931, 1886
PstNI CAGNNNCTG 1 cut(s) 1636
PsuI RGATCY 6 cut(s) 135, 145, 316, 625, 960, 1809
RsaI GTAC 9 cut(s) 119, 125, 461, 554, 773, 834, 903, 1130, 1836
RsaNI GTAC 9 cut(s) 118, 124, 460, 553, 772, 833, 902, 1129, 1835
RseI CAYNNNNRTG 2 cut(s) 762, 1280
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 6 cut(s) 266, 693, 1041, 1662, 1692, 1733
SatI GCNGC 1 cut(s) 1800
Sau96I GGNCC 2 cut(s) 931, 1886
ScaI AGTACT 2 cut(s) 119, 834
SchI GAGTC 3 cut(s) 650, 1627, 1787
ScrFI CCNGG 5 cut(s) 97, 566, 906, 935, 1073
SduI GDGCHC 1 cut(s) 252
SfaNI GCATC 1 cut(s) 1509
SfuI TTCGAA 1 cut(s) 217
SinI GGWCC 1 cut(s) 1886
SmiMI CAYNNNNRTG 2 cut(s) 762, 1280
SpeI ACTAGT 1 cut(s) 1177
SphI GCATGC 1 cut(s) 1675
SsiI CCGC 4 cut(s) 142, 542, 1408, 1582
SspMI CTAG 4 cut(s) 18, 857, 1178, 1851
StyD4I CCNGG 5 cut(s) 95, 564, 904, 933, 1071
StyI CCWWGG 2 cut(s) 1316, 1425
TaaI ACNGT 2 cut(s) 776, 901
TaiI ACGT 1 cut(s) 1059
TaqI TCGA 7 cut(s) 217, 546, 574, 589, 654, 1368, 1896
TatI WGTACW 5 cut(s) 117, 771, 832, 1128, 1834
TfiI GAWTC 5 cut(s) 425, 591, 860, 1657, 1784
Tru1I TTAA 6 cut(s) 266, 693, 1041, 1662, 1692, 1733
Tru9I TTAA 6 cut(s) 266, 693, 1041, 1662, 1692, 1733
TscAI CASTG 3 cut(s) 88, 781, 1492
TseFI GTSAC 2 cut(s) 560, 1229
TseI GCWGC 1 cut(s) 1799
Tsp45I GTSAC 2 cut(s) 560, 1229
TspDTI ATGAA 8 cut(s) 174, 371, 780, 999, 1148, 1164, 1390, 1883
TspGWI ACGGA 2 cut(s) 447, 1565
TspRI CASTG 3 cut(s) 88, 781, 1492
Van91I CCANNNNNTGG 2 cut(s) 1322, 1480
VneI GTGCAC 1 cut(s) 248
VpaK11BI GGWCC 1 cut(s) 1886
VspI ATTAAT 1 cut(s) 1692
XapI RAATTY 4 cut(s) 677, 744, 1064, 1892
XceI RCATGY 1 cut(s) 1675
XcmI CCANNNNNNNNNTGG 1 cut(s) 1820
XmnI GAANNNNTTC 2 cut(s) 1380, 1565
XspI CTAG 4 cut(s) 18, 857, 1178, 1851
ZrmI AGTACT 2 cut(s) 119, 834
Zsp2I ATGCAT 2 cut(s) 418, 1847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.