Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0001714

Physical Location & Seq

Genomic Coordinates Reverse (-)

23633681 .. 23638165

Gene Structure 4485 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g30550.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1656 bp

ATGTCAGAACCTCAGCCAGCCTCCGAGCTCAGCCACGAGCCTGAGAACGGCGAGAACGGAACGAACGGAGACGCCGCCTCAGTGAGCAAAAAGGCGGCAAAGAAAGAAGCCGCCAAGCAAGAGAAGCTCCGCCGCCGCCAGGAGCAAGAATCGGCCGCCGCCGCCGCCCGCGCGCAGTCCGCTGACTCGGAGGACCCGCTCGCCGAGAACTACGGCGACTTGCCGCTGATTCAGCTCCAATCGATGAGGATCGCCGACGTCAAGGACTGGACTGAGGTCGGAGCTCTGACGGAGGCGTTGAAGGACAAGCAGGTTCAGATACGCGGGCGCGCGCAGACGATCCGAGCCGTCGGGAAGAACATGGCGTTCATTGTGGTGAGAGAGAAGGGGTTCACGGTTCAGTGCGTCGCGACGGTCCAGCCGGAGATTGTGAGCCGTCAGATGGTGAAATACGTGGCGGGGCTGAGCCGCGAGTCGATTATTGACATTGAAGGGATTGTGTCTGTTCCTGGAACGCCGATTATAAGCGCTTCACAGCAGGTGGAGGTGCAAGTCAGAAAGGTGTACTGTATTAGTAGGGCGGCTGTTTTGCCTATTAATGTTGAGGATGCTGCTCGAAGTGATGCCGAGATTGAAAGAGCTAATCAGGCTGGAGAACAAATGGTTCGTGTGAACCAGGACACTCGACTGAACTACCGAGTACTCGACTTGCGCACACCAGCTAACCAAGGGATCTTCAGAATCCAGAGTCGAGTTGGAACTATCTTCAGGGAGTTCCTGCTGTCCCAAGGGTTTTTTGAAATCCACTCACCCAAACTGATTGCTGGTTCAAGTGAAGGTGGGGCTTCGGTGTTCAAGCTGGACTACAAGGGGCAACCTGCCTGCCTTGCCCAGTCACCTCAGCTCCACAAGCAGATGTCCATTTGTGGTGACTTTGGACGTATTTTTGAGATCGGACCTGTTTTCAGAGCTGAGGACTCCTACACACACAGGCATCTATGTGAGTTCACTGGTCTTGATGTCGAGATGGAGATTAAGTGGAGCTACTTCGAGGTTATGGATGTTGTTGACAAGTTGTTTGTTACAATGTTCAACTATTTGAACCAAAATTGCGAGAAAGAATTTGATGCCGTAAGGAGGCAGTATCCCTTTGAACCGCTTAAGTATCTACCTGAGACTCTACGTCTTACTTTTGCGGAAGGTGTTCAAATGCTAAAGGATGCTGGTGTTGAAGTTGATCCAATGGGGGACCTTAATACCGAGGCGGAGAGGAAGTTGGGCCAATTAGTTCTGGAAAAATATAACACTGAGTTCTACATCCTTCATCGATATCCATTGGCTGTGAGGCCGTTTTACACCATGCCTTGCCATGACAATAAGGAGTACAGTAATTCATTTGACGTCTTCATTCGAGGTGAGGAGATTATATCCGGAGCGCAACGTGTCCATGTGCCGGACTTCTTGGCTGAACGTGCTGAAGCACGCGGTATTGATGTCAAGACAATATCAACATACATTGATTCCTTCAGATATGGTGCACCTCCCCATGGTGGATTTGGAGTAGGGCTGGAGCGTGTGGTGATGCTCTTCTGTGGGTTGAACAACATCCGCAAGACATCCTTATTCCCGCGTGATCCGCTCAGGCTAGCTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004815 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006422 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0012505 GO:0016070 GO:0016874 GO:0016875 GO:0017101 GO:0019538 GO:0019752 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045202 GO:0046483 GO:0046686 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0090304 GO:0098542 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2 tRNA_anti-codon

Protein Analysis

552

Amino Acids

61.85

Weight (kDa)

5.79

Isoelectric Point (pI)

43.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2	PF00152	225 - 545	1.2e-78	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001714)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26870 AT4G31180 AT4G31180
fragaria_vesca	FvH4_2g15870 FvH4_2g15910 FvH4_2g30550
malus_domestica	MD08G1134400.v1.1 MD12G1153800.v1.1 MD14G1143300.v1.1 MD15G1113400.v1.1
prunus_persica	Prupe.1G463800_v2.0.a1 Prupe.4G141600_v2.0.a1 Prupe.8G055000_v2.0.a1
pyrus_communis	pycom08g11340 pycom15g10170 pycom16g11710
rosa_chinensis	RchiOBHm_Chr5g0032141 RchiOBHm_Chr5g0032161 RchiOBHm_Chr6g0278871 RchiOBHm_Chr6g0312531
rosa_laevigata	RLG00000010321 RLG00000033368
rosa_multiflora	Rmu_co8427103.1_g000001 Rmu_sc0003031.1_g000023 Rmu_sc0005375.1_g000002 Rmu_sc0005375.1_g000011 Rmu_sc0008363.1_g000011 Rmu_sc0043080.1_g000001
rosa_roxburghii	Rroxscaffold_1G00048240 Rroxscaffold_1G00048270 Rroxscaffold_7G00156770
rosa_rugosa	Rorug06G0119800 Rorug06G0120000 Rorug06G0123400 Rorug06G0396100 Rorug06G0396200
rosa_samantha	Rh5DG228900 Rh6AG228300 Rh6AG513500 Rh6BG233900 Rh6BG234000 Rh6BG524200 Rh6BG526500 Rh6CG236300 Rh6CG531400 Rh6DG515600
rosa_wichuraiana	Rw6G020030 Rw6G044650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	524
AarI	CACCTGC	1 cut(s)	529
AatII	GACGTC	2 cut(s)	261, 1404
Acc16I	TGCGCA	1 cut(s)	713
Acc36I	ACCTGC	3 cut(s)	301, 529, 886
AccBSI	CCGCTC	2 cut(s)	199, 1639
AccII	CGCG	9 cut(s)	171, 173, 324, 330, 332, 410, 471, 1485, 1630
AccIII	TCCGGA	1 cut(s)	1430
AclWI	GGATC	5 cut(s)	257, 334, 740, 1232, 1628
AcoI	YGGCCR	1 cut(s)	153
AcsI	RAATTY	1 cut(s)	1121
AcuI	CTGAAG	4 cut(s)	721, 751, 1497, 1510
AcyI	GRCGYC	3 cut(s)	72, 258, 1401
AfaI	GTAC	3 cut(s)	566, 702, 1385
AfeI	AGCGCT	1 cut(s)	529
AfiI	CCNNNNNNNGG	7 cut(s)	47, 139, 442, 1137, 1453, 1547, 1550
AflII	CTTAAG	1 cut(s)	1160
AflIII	ACRYGT	1 cut(s)	1441
AhdI	GACNNNNNGTC	1 cut(s)	1182
AjnI	CCWGG	3 cut(s)	138, 508, 675
Alw21I	GWGCWC	3 cut(s)	30, 286, 1540
Alw26I	GTCTC	2 cut(s)	63, 1169
Alw44I	GTGCAC	1 cut(s)	1536
AlwI	GGATC	5 cut(s)	257, 334, 740, 1232, 1628
Aor13HI	TCCGGA	1 cut(s)	1430
Aor51HI	AGCGCT	1 cut(s)	529
AoxI	GGCC	3 cut(s)	153, 1279, 1346
ApaLI	GTGCAC	1 cut(s)	1536
ApeKI	GCWGC	1 cut(s)	611
ApoI	RAATTY	1 cut(s)	1121
ArsI	GACNNNNNNTTYG	2 cut(s)	1061, 1093
AseI	ATTAAT	1 cut(s)	597
Asp700I	GAANNNNTTC	2 cut(s)	389, 1203
AspLEI	GCGC	8 cut(s)	173, 175, 330, 332, 334, 530, 714, 1438
AspS9I	GGNCC	5 cut(s)	193, 415, 956, 1249, 1279
AsuHPI	GGTGA	7 cut(s)	388, 457, 801, 888, 941, 1427, 1591
AsuNHI	GCTAGC	1 cut(s)	1645
AvaII	GGWCC	4 cut(s)	193, 415, 956, 1249
BaeGI	GKGCMC	1 cut(s)	1540
BanII	GRGCYC	2 cut(s)	30, 286
BauI	CACGAG	1 cut(s)	35
BbsI	GAAGAC	1 cut(s)	1396
Bbv12I	GWGCWC	3 cut(s)	30, 286, 1540
BbvCI	CCTCAGC	3 cut(s)	12, 900, 972
BbvI	GCAGC	1 cut(s)	598
BccI	CCATC	2 cut(s)	436, 1021
BceAI	ACGGC	6 cut(s)	64, 229, 332, 420, 1115, 1333
BciT130I	CCWGG	3 cut(s)	140, 510, 677
BciVI	GTATCC	1 cut(s)	1155
BcoDI	GTCTC	2 cut(s)	63, 1169
BfaI	CTAG	1 cut(s)	1646
BfoI	RGCGCY	1 cut(s)	531
BfrI	CTTAAG	1 cut(s)	1160
BfuAI	ACCTGC	3 cut(s)	301, 529, 886
BfuI	GTATCC	1 cut(s)	1155
BlpI	GCTNAGC	2 cut(s)	29, 464
BmcAI	AGTACT	1 cut(s)	702
Bme1390I	CCNGG	3 cut(s)	140, 510, 677
Bme18I	GGWCC	4 cut(s)	193, 415, 956, 1249
BmeRI	GACNNNNNGTC	1 cut(s)	1182
BmgT120I	GGNCC	5 cut(s)	193, 415, 956, 1249, 1279
BmiI	GGNNCC	2 cut(s)	195, 1250
BmrFI	CCNGG	3 cut(s)	140, 510, 677
BmrI	ACTGGG	1 cut(s)	886
BmsI	GCATC	6 cut(s)	598, 613, 1003, 1117, 1210, 1572
BmtI	GCTAGC	1 cut(s)	1649
BmuI	ACTGGG	1 cut(s)	886
BoxI	GACNNNNGTC	1 cut(s)	275
BpiI	GAAGAC	1 cut(s)	1396
BpmI	CTGGAG	2 cut(s)	672, 1589
Bpu10I	CCTNAGC	4 cut(s)	12, 900, 972, 1640
Bpu1102I	GCTNAGC	2 cut(s)	29, 464
Bsa29I	ATCGAT	2 cut(s)	242, 1327
BsaAI	YACGTR	1 cut(s)	454
BsaBI	GATNNNNATC	1 cut(s)	248
BsaHI	GRCGYC	3 cut(s)	72, 258, 1401
BsaJI	CCNNGG	4 cut(s)	727, 787, 1260, 1546
BsaWI	WCCGGW	1 cut(s)	1430
BsaXI	ACNNNNNCTCC	6 cut(s)	888, 918, 1022, 1052, 1259, 1289
Bsc4I	CCNNNNNNNGG	7 cut(s)	47, 139, 442, 1137, 1453, 1547, 1550
Bse1I	ACTGG	3 cut(s)	272, 892, 1015
Bse8I	GATNNNNATC	1 cut(s)	248
BseAI	TCCGGA	1 cut(s)	1430
BseBI	CCWGG	3 cut(s)	140, 510, 677
BseCI	ATCGAT	2 cut(s)	242, 1327
BseDI	CCNNGG	4 cut(s)	727, 787, 1260, 1546
BseGI	GGATG	6 cut(s)	613, 1066, 1225, 1317, 1605, 1616
BseJI	GATNNNNATC	1 cut(s)	248
BseLI	CCNNNNNNNGG	7 cut(s)	47, 139, 442, 1137, 1453, 1547, 1550
BseNI	ACTGG	3 cut(s)	272, 892, 1015
BsePI	GCGCGC	3 cut(s)	171, 328, 330
BseRI	GAGGAG	1 cut(s)	1433
BseSI	GKGCMC	1 cut(s)	1540
BseX3I	CGGCCG	1 cut(s)	153
BseXI	GCAGC	1 cut(s)	598
Bsh1236I	CGCG	9 cut(s)	171, 173, 324, 330, 332, 410, 471, 1485, 1630
Bsh1285I	CGRYCG	1 cut(s)	156
BshFI	GGCC	3 cut(s)	155, 1281, 1348
BshVI	ATCGAT	2 cut(s)	242, 1327
BsiEI	CGRYCG	1 cut(s)	156
BsiHKAI	GWGCWC	3 cut(s)	30, 286, 1540
BsiSI	CCGG	3 cut(s)	422, 1431, 1454
BslFI	GGGAC	2 cut(s)	769, 1262
BslI	CCNNNNNNNGG	7 cut(s)	47, 139, 442, 1137, 1453, 1547, 1550
BsmAI	GTCTC	2 cut(s)	63, 1169
BsmBI	CGTCTC	1 cut(s)	63
BsmFI	GGGAC	2 cut(s)	769, 1262
BsnI	GGCC	3 cut(s)	155, 1281, 1348
Bsp1286I	GDGCHC	3 cut(s)	30, 286, 1540
Bsp13I	TCCGGA	1 cut(s)	1430
Bsp143I	GATC	6 cut(s)	249, 339, 732, 951, 1237, 1633
Bsp1720I	GCTNAGC	2 cut(s)	29, 464
Bsp19I	CCATGG	1 cut(s)	1546
Bsp68I	TCGCGA	1 cut(s)	410
BspANI	GGCC	3 cut(s)	155, 1281, 1348
BspDI	ATCGAT	2 cut(s)	242, 1327
BspEI	TCCGGA	1 cut(s)	1430
BspFNI	CGCG	9 cut(s)	171, 173, 324, 330, 332, 410, 471, 1485, 1630
BspLI	GGNNCC	2 cut(s)	195, 1250
BspMI	ACCTGC	3 cut(s)	301, 529, 886
BspOI	GCTAGC	1 cut(s)	1649
BspPI	GGATC	5 cut(s)	257, 334, 740, 1232, 1628
BspQI	GCTCTTC	1 cut(s)	1592
BspTI	CTTAAG	1 cut(s)	1160
BsrBI	CCGCTC	2 cut(s)	199, 1639
BsrI	ACTGG	3 cut(s)	272, 892, 1015
BssECI	CCNNGG	4 cut(s)	727, 787, 1260, 1546
BssHII	GCGCGC	3 cut(s)	171, 328, 330
BssMI	GATC	6 cut(s)	249, 339, 732, 951, 1237, 1633
BssNI	GRCGYC	3 cut(s)	72, 258, 1401
BssSI	CACGAG	1 cut(s)	35
BssT1I	CCWWGG	3 cut(s)	727, 787, 1546
Bst2BI	CACGAG	1 cut(s)	35
Bst2UI	CCWGG	3 cut(s)	140, 510, 677
Bst4CI	ACNGT	4 cut(s)	397, 415, 569, 1388
Bst6I	CTCTTC	1 cut(s)	1592
BstACI	GRCGYC	3 cut(s)	72, 258, 1401
BstAFI	CTTAAG	1 cut(s)	1160
BstBAI	YACGTR	1 cut(s)	454
BstDSI	CCRYGG	1 cut(s)	1546
BstF5I	GGATG	6 cut(s)	613, 1066, 1225, 1317, 1605, 1616
BstFNI	CGCG	9 cut(s)	171, 173, 324, 330, 332, 410, 471, 1485, 1630
BstH2I	RGCGCY	1 cut(s)	531
BstHHI	GCGC	8 cut(s)	173, 175, 330, 332, 334, 530, 714, 1438
BstKTI	GATC	6 cut(s)	252, 342, 735, 954, 1240, 1636
BstMAI	GTCTC	2 cut(s)	63, 1169
BstMBI	GATC	6 cut(s)	249, 339, 732, 951, 1237, 1633
BstMCI	CGRYCG	1 cut(s)	156
BstNI	CCWGG	3 cut(s)	140, 510, 677
BstPAI	GACNNNNGTC	1 cut(s)	275
BstSCI	CCNGG	3 cut(s)	138, 508, 675
BstSLI	GKGCMC	1 cut(s)	1540
BstUI	CGCG	9 cut(s)	171, 173, 324, 330, 332, 410, 471, 1485, 1630
BstV1I	GCAGC	1 cut(s)	598
BstV2I	GAAGAC	1 cut(s)	1396
BstX2I	RGATCY	1 cut(s)	732
BstYI	RGATCY	1 cut(s)	732
BstZI	CGGCCG	1 cut(s)	153
Bsu15I	ATCGAT	2 cut(s)	242, 1327
BsuI	GTATCC	1 cut(s)	1155
BsuRI	GGCC	3 cut(s)	155, 1281, 1348
BsuTUI	ATCGAT	2 cut(s)	242, 1327
BtgI	CCRYGG	1 cut(s)	1546
BtsCI	GGATG	6 cut(s)	613, 1066, 1225, 1317, 1605, 1616
BtsIMutI	CAGTG	4 cut(s)	87, 407, 1008, 1305
BtuMI	TCGCGA	1 cut(s)	410
BveI	ACCTGC	3 cut(s)	301, 529, 886
CfoI	GCGC	8 cut(s)	173, 175, 330, 332, 334, 530, 714, 1438
Cfr13I	GGNCC	5 cut(s)	193, 415, 956, 1249, 1279
ClaI	ATCGAT	2 cut(s)	242, 1327
CseI	GACGC	2 cut(s)	80, 394
Csp6I	GTAC	3 cut(s)	565, 701, 1384
CviAII	CATG	5 cut(s)	361, 1360, 1370, 1448, 1547
CviQI	GTAC	3 cut(s)	565, 701, 1384
DpnI	GATC	6 cut(s)	251, 341, 734, 953, 1239, 1635
DpnII	GATC	6 cut(s)	249, 339, 732, 951, 1237, 1633
DriI	GACNNNNNGTC	1 cut(s)	1182
EaeI	YGGCCR	1 cut(s)	153
EagI	CGGCCG	1 cut(s)	153
Eam1104I	CTCTTC	1 cut(s)	1592
Eam1105I	GACNNNNNGTC	1 cut(s)	1182
EarI	CTCTTC	1 cut(s)	1592
EciI	GGCGGA	2 cut(s)	119, 1280
Ecl136II	GAGCTC	2 cut(s)	28, 284
EclXI	CGGCCG	1 cut(s)	153
Eco130I	CCWWGG	3 cut(s)	727, 787, 1546
Eco24I	GRGCYC	2 cut(s)	30, 286
Eco32I	GATATC	1 cut(s)	1331
Eco47I	GGWCC	4 cut(s)	193, 415, 956, 1249
Eco47III	AGCGCT	1 cut(s)	529
Eco52I	CGGCCG	1 cut(s)	153
Eco53kI	GAGCTC	2 cut(s)	28, 284
Eco57I	CTGAAG	4 cut(s)	721, 751, 1497, 1510
EcoICRI	GAGCTC	2 cut(s)	28, 284
EcoO109I	RGGNCCY	2 cut(s)	193, 1249
EcoRII	CCWGG	3 cut(s)	138, 508, 675
EcoRV	GATATC	1 cut(s)	1331
EcoT14I	CCWWGG	3 cut(s)	727, 787, 1546
EcoT38I	GRGCYC	2 cut(s)	30, 286
ErhI	CCWWGG	3 cut(s)	727, 787, 1546
Esp3I	CGTCTC	1 cut(s)	63
FaeI	CATG	5 cut(s)	364, 1363, 1373, 1451, 1550
FalI	AAGNNNNNCTT	2 cut(s)	1604, 1636
FaqI	GGGAC	2 cut(s)	769, 1262
FatI	CATG	5 cut(s)	360, 1359, 1369, 1447, 1546
FauI	CCCGC	5 cut(s)	176, 204, 317, 451, 1635
FokI	GGATG	6 cut(s)	620, 1073, 1232, 1304, 1592, 1603
FriOI	GRGCYC	2 cut(s)	30, 286
FspBI	CTAG	1 cut(s)	1646
FspI	TGCGCA	1 cut(s)	713
GlaI	GCGC	8 cut(s)	172, 174, 329, 331, 333, 529, 713, 1437
GsuI	CTGGAG	2 cut(s)	672, 1589
HaeII	RGCGCY	1 cut(s)	531
HaeIII	GGCC	3 cut(s)	155, 1281, 1348
HapII	CCGG	3 cut(s)	422, 1431, 1454
HgaI	GACGC	2 cut(s)	80, 394
HhaI	GCGC	8 cut(s)	173, 175, 330, 332, 334, 530, 714, 1438
Hin1I	GRCGYC	3 cut(s)	72, 258, 1401
Hin1II	CATG	5 cut(s)	364, 1363, 1373, 1451, 1550
Hin6I	GCGC	8 cut(s)	171, 173, 328, 330, 332, 528, 712, 1436
HinP1I	GCGC	8 cut(s)	171, 173, 328, 330, 332, 528, 712, 1436
HincII	GTYRAC	1 cut(s)	1069
HindII	GTYRAC	1 cut(s)	1069
HinfI	GANTC	9 cut(s)	149, 185, 229, 473, 741, 748, 977, 1177, 1520
HpaII	CCGG	3 cut(s)	422, 1431, 1454
HphI	GGTGA	7 cut(s)	388, 457, 801, 888, 941, 1427, 1591
Hpy166II	GTNNAC	6 cut(s)	393, 565, 673, 1008, 1069, 1538
Hpy188III	TCNNGA	8 cut(s)	352, 409, 745, 1016, 1024, 1292, 1431, 1498
Hpy8I	GTNNAC	6 cut(s)	393, 565, 673, 1008, 1069, 1538
Hpy99I	CGWCG	4 cut(s)	260, 353, 410, 415
HpyAV	CCTTC	7 cut(s)	295, 379, 485, 830, 1193, 1331, 1534
HpyCH4III	ACNGT	4 cut(s)	397, 415, 569, 1388
HpyCH4IV	ACGT	7 cut(s)	258, 453, 940, 1183, 1401, 1441, 1471
HpyCH4V	TGCA	2 cut(s)	550, 1538
HpySE526I	ACGT	7 cut(s)	258, 453, 940, 1183, 1401, 1441, 1471
Hsp92I	GRCGYC	3 cut(s)	72, 258, 1401
Hsp92II	CATG	5 cut(s)	364, 1363, 1373, 1451, 1550
HspAI	GCGC	8 cut(s)	171, 173, 328, 330, 332, 528, 712, 1436
Kpn2I	TCCGGA	1 cut(s)	1430
Kzo9I	GATC	6 cut(s)	249, 339, 732, 951, 1237, 1633
LguI	GCTCTTC	1 cut(s)	1592
LmnI	GCTCC	9 cut(s)	132, 142, 240, 281, 909, 1041, 1433, 1570, 1654
Lsp1109I	GCAGC	1 cut(s)	598
LweI	GCATC	6 cut(s)	598, 613, 1003, 1117, 1210, 1572
MaeI	CTAG	1 cut(s)	1646
MaeII	ACGT	7 cut(s)	258, 453, 940, 1183, 1401, 1441, 1471
MaeIII	GTNAC	3 cut(s)	894, 929, 1081
MalI	GATC	6 cut(s)	251, 341, 734, 953, 1239, 1635
MbiI	CCGCTC	2 cut(s)	199, 1639
MboI	GATC	6 cut(s)	249, 339, 732, 951, 1237, 1633
MboII	GAAGA	5 cut(s)	367, 727, 757, 1396, 1579
MflI	RGATCY	1 cut(s)	732
MhlI	GDGCHC	3 cut(s)	30, 286, 1540
MluCI	AATT	4 cut(s)	1108, 1121, 1283, 1390
MlyI	GAGTC	5 cut(s)	179, 482, 757, 971, 1171
MmeI	TCCRAC	2 cut(s)	259, 736
MroI	TCCGGA	1 cut(s)	1430
MroXI	GAANNNNTTC	2 cut(s)	389, 1203
MseI	TTAA	4 cut(s)	597, 1035, 1161, 1254
MslI	CAYNNNNRTG	2 cut(s)	374, 999
MspA1I	CMGCKG	2 cut(s)	182, 226
MspCI	CTTAAG	1 cut(s)	1160
MspI	CCGG	3 cut(s)	422, 1431, 1454
MspR9I	CCNGG	3 cut(s)	140, 510, 677
MvaI	CCWGG	3 cut(s)	140, 510, 677
MvnI	CGCG	9 cut(s)	171, 173, 324, 330, 332, 410, 471, 1485, 1630
NcoI	CCATGG	1 cut(s)	1546
NdeII	GATC	6 cut(s)	249, 339, 732, 951, 1237, 1633
NheI	GCTAGC	1 cut(s)	1645
NlaIII	CATG	5 cut(s)	364, 1363, 1373, 1451, 1550
NlaIV	GGNNCC	2 cut(s)	195, 1250
NmeAIII	GCCGAG	2 cut(s)	229, 652
NmuCI	GTSAC	2 cut(s)	894, 929
NruI	TCGCGA	1 cut(s)	410
NsbI	TGCGCA	1 cut(s)	713
PaqCI	CACCTGC	1 cut(s)	529
PauI	GCGCGC	3 cut(s)	171, 328, 330
PciSI	GCTCTTC	1 cut(s)	1592
PdmI	GAANNNNTTC	2 cut(s)	389, 1203
PfeI	GAWTC	4 cut(s)	149, 229, 741, 1520
PfoI	TCCNGGA	1 cut(s)	508
PleI	GAGTC	5 cut(s)	179, 481, 756, 971, 1171
PpsI	GAGTC	5 cut(s)	179, 481, 756, 971, 1171
Ppu21I	YACGTR	1 cut(s)	454
PpuMI	RGGWCCY	2 cut(s)	193, 1249
PshAI	GACNNNNGTC	1 cut(s)	275
PshBI	ATTAAT	1 cut(s)	597
PsiI	TTATAA	1 cut(s)	524
Psp124BI	GAGCTC	2 cut(s)	30, 286
Psp5II	RGGWCCY	2 cut(s)	193, 1249
Psp6I	CCWGG	3 cut(s)	138, 508, 675
PspGI	CCWGG	3 cut(s)	138, 508, 675
PspN4I	GGNNCC	2 cut(s)	195, 1250
PspPI	GGNCC	5 cut(s)	193, 415, 956, 1249, 1279
PspPPI	RGGWCCY	2 cut(s)	193, 1249
PsuI	RGATCY	1 cut(s)	732
PteI	GCGCGC	3 cut(s)	171, 328, 330
RruI	TCGCGA	1 cut(s)	410
RsaI	GTAC	3 cut(s)	566, 702, 1385
RsaNI	GTAC	3 cut(s)	565, 701, 1384
RseI	CAYNNNNRTG	2 cut(s)	374, 999
SacI	GAGCTC	2 cut(s)	30, 286
SapI	GCTCTTC	1 cut(s)	1592
SaqAI	TTAA	4 cut(s)	597, 1035, 1161, 1254
Sau3AI	GATC	6 cut(s)	249, 339, 732, 951, 1237, 1633
Sau96I	GGNCC	5 cut(s)	193, 415, 956, 1249, 1279
ScaI	AGTACT	1 cut(s)	702
SchI	GAGTC	5 cut(s)	179, 482, 757, 971, 1171
ScrFI	CCNGG	3 cut(s)	140, 510, 677
SduI	GDGCHC	3 cut(s)	30, 286, 1540
SfaNI	GCATC	6 cut(s)	598, 613, 1003, 1117, 1210, 1572
SinI	GGWCC	4 cut(s)	193, 415, 956, 1249
SmiMI	CAYNNNNRTG	2 cut(s)	374, 999
SmlI	CTYRAG	1 cut(s)	1160
SmoI	CTYRAG	1 cut(s)	1160
Sse9I	AATT	4 cut(s)	1108, 1121, 1283, 1390
SspMI	CTAG	1 cut(s)	1646
SstI	GAGCTC	2 cut(s)	30, 286
StyD4I	CCNGG	3 cut(s)	138, 508, 675
StyI	CCWWGG	3 cut(s)	727, 787, 1546
TaaI	ACNGT	4 cut(s)	397, 415, 569, 1388
TaiI	ACGT	7 cut(s)	261, 456, 943, 1186, 1404, 1444, 1474
TasI	AATT	4 cut(s)	1108, 1121, 1283, 1390
TatI	WGTACW	3 cut(s)	564, 700, 1383
TfiI	GAWTC	4 cut(s)	149, 229, 741, 1520
Tru1I	TTAA	4 cut(s)	597, 1035, 1161, 1254
Tru9I	TTAA	4 cut(s)	597, 1035, 1161, 1254
TscAI	CASTG	4 cut(s)	87, 407, 1015, 1312
TseFI	GTSAC	2 cut(s)	894, 929
TseI	GCWGC	1 cut(s)	611
Tsp45I	GTSAC	2 cut(s)	894, 929
TspDTI	ATGAA	4 cut(s)	358, 1313, 1383, 1396
TspGWI	ACGGA	3 cut(s)	72, 81, 305
TspRI	CASTG	4 cut(s)	87, 407, 1015, 1312
Vha464I	CTTAAG	1 cut(s)	1160
VneI	GTGCAC	1 cut(s)	1536
VpaK11BI	GGWCC	4 cut(s)	193, 415, 956, 1249
VspI	ATTAAT	1 cut(s)	597
XapI	RAATTY	1 cut(s)	1121
XcmI	CCANNNNNNNNNTGG	2 cut(s)	752, 1553
XmnI	GAANNNNTTC	2 cut(s)	389, 1203
XspI	CTAG	1 cut(s)	1646
ZraI	GACGTC	2 cut(s)	259, 1402
ZrmI	AGTACT	1 cut(s)	702

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.