Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001714

Physical Location & Seq

Genomic Coordinates Reverse (-)

7970558 .. 7974320

Gene Structure 3763 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1113400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGTCGACACCGGAATCTCAACCCCCCGCCGGCTCCGAGCCATCCAACGAAGACTCCGCCTCCGTCAGCAAAAAAGCAGCTAAAAAAGAAGCCGCCAAGCAAGAGAAGTTGCGCCGCCGCCAGGAGCAGGAAGCCCTAGCGGCCGCGGCTCGTTCCCTCTCCGTCGACGAGCAGGATCCTCTCGCTGCGAACTACGGCGACGTCCCCCTGATCGAGCTCCAATCGAAGACGGCTGAGGACGTCAGTCACTGGACCGAGGTCGGAAGCCTAACCAATGCGTTGGAGAACCGGTCCGTGCTGATACGTGGCCGGGCACAGACGATTCGTGCCGTCGGGAAGAATATGGCGTTTGTTGTCGTCAGAGAAAGGGGGTTTACGGTGCAGTGCGTGGCGACGGTGCAGCCTGACACCGTGAGCCGCCAGATGGTGAAGTACATCGCTGGGTTGAGCAGAGAGTCGATTATCGATATTGAAGGCGTCGTGTCTGTTCCTAGTGTCGAGATTAAAGGCACCACGCAGCAGGTGGAGGTTCAAGTGAGGAAGTTGTATTGTGTGAGTAAGGCTGCTGTTTTACCTATTAACATTGAGGATGCTGCTCGGAGTGATGTCGAAATCGAAAAGGCTTTGCAGGCCGGAGAAACACTAGTTCGGGTTAATCAGGATACCCGCTTGAATAACCGAGTTCTTGATTTGCGAACACCCGCGAATCAGGGGATATTCCGCATTCAGAGTCAAGTTGGCAATATCTTTAGGCAGTTCTTGATATCCGAAGGCTTTTTTGAAATCCACTCGCCAAAGCTGATTGCTGGTTCAAGTGAAGGTGGAGCTGCTGTTTTTAGACTCAACTACAAGGGTCAAGAAGCCTGCCTCGCCCAGTCACCTCAGCTTCACAAGCAGATGGCTATCTGTGGTGACTTCGGCCGTATTTTTGAGATTGGTCCTGTTTTTAGAGCTGAGGACTCCTTTACCCACAGGCATTTGTGTGAGTTCACTGGCCTTGATGTCGAGATGGAGATCAAGAGGCACTACTCTGAGGTCATGGACCTTGTTGGTCGCTTGTTTGTTGCGATGTTCGACTCCTTGAACAAGAACTGTGCAAAAGAGCTTGAAGCTGTAGCGAGGCAGTATCCATTCCAACCGCTAAAGTATTTGCCAGAGACACTATGTTTAAAATTTGAAGAAGGTGTTCAAATGCTTAAGGAATCTGGGGTTGAAGTGGATCCTTTTGGGGATTTAAACACTGAGAATGAGAGAAAATTGGGCCTACTAGTTTTGGAGAAGTATGGCACCGAGTTCTACATACTGCACCGCTATCCTTTGGCTGTTAGGCCATTCTACACAATGCCTTGCCATGACAATCCTGCTTACAGTAATTCGTTTGATGTTTTTATTCGAGGCGAGGAGATAATTTCTGGAGCACAGCGTGTCCATGTTCCAGAGTTCTTGACTGAGCGTGCACAGGCGTGTGGAATTGATGTAAAGACGATACAAACATATATCGACGCTTTCAGATACGGTGCACCTCCCCACGGTGGATTTGGAGTAGGGCTGGAGCGTGTCGTCATGCTTTTTTGTGGTTTGAACAACATCCGCAAAACTTCTCTGTTTCCTCGTGATCCACTTAGGCTCGCCCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004815 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006422 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0012505 GO:0016070 GO:0016874 GO:0016875 GO:0017101 GO:0019538 GO:0019752 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045202 GO:0046483 GO:0046686 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0090304 GO:0098542 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2 tRNA_anti-codon

Protein Analysis

546

Amino Acids

60.75

Weight (kDa)

6.04

Isoelectric Point (pI)

54.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2	PF00152	219 - 539	3e-77	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001714)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26870 AT4G31180 AT4G31180
fragaria_vesca	FvH4_2g15870 FvH4_2g15910 FvH4_2g30550
malus_domestica	MD08G1134400.v1.1 MD12G1153800.v1.1 MD14G1143300.v1.1 MD15G1113400.v1.1
prunus_persica	Prupe.1G463800_v2.0.a1 Prupe.4G141600_v2.0.a1 Prupe.8G055000_v2.0.a1
pyrus_communis	pycom08g11340 pycom15g10170 pycom16g11710
rosa_chinensis	RchiOBHm_Chr5g0032141 RchiOBHm_Chr5g0032161 RchiOBHm_Chr6g0278871 RchiOBHm_Chr6g0312531
rosa_laevigata	RLG00000010321 RLG00000033368
rosa_multiflora	Rmu_co8427103.1_g000001 Rmu_sc0003031.1_g000023 Rmu_sc0005375.1_g000002 Rmu_sc0005375.1_g000011 Rmu_sc0008363.1_g000011 Rmu_sc0043080.1_g000001
rosa_roxburghii	Rroxscaffold_1G00048240 Rroxscaffold_1G00048270 Rroxscaffold_7G00156770
rosa_rugosa	Rorug06G0119800 Rorug06G0120000 Rorug06G0123400 Rorug06G0396100 Rorug06G0396200
rosa_samantha	Rh5DG228900 Rh6AG228300 Rh6AG513500 Rh6BG233900 Rh6BG234000 Rh6BG524200 Rh6BG526500 Rh6CG236300 Rh6CG531400 Rh6DG515600
rosa_wichuraiana	Rw6G020030 Rw6G044650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	511
AatII	GACGTC	2 cut(s)	204, 243
Acc36I	ACCTGC	1 cut(s)	511
AccB1I	GGYRCC	2 cut(s)	509, 1286
AccI	GTMKAC	2 cut(s)	5, 165
AccII	CGCG	2 cut(s)	146, 704
AclWI	GGATC	5 cut(s)	170, 183, 1214, 1227, 1610
AcoI	YGGCCR	3 cut(s)	141, 307, 919
AcsI	RAATTY	1 cut(s)	1172
AcyI	GRCGYC	3 cut(s)	201, 240, 477
AdeI	CACNNNGTG	1 cut(s)	1424
AfaI	GTAC	1 cut(s)	434
AfiI	CCNNNNNNNGG	6 cut(s)	29, 121, 127, 424, 1529, 1532
AflII	CTTAAG	1 cut(s)	1196
AgeI	ACCGGT	1 cut(s)	288
AhlI	ACTAGT	2 cut(s)	643, 1267
AjnI	CCWGG	1 cut(s)	120
AleI	CACNNNNGTG	1 cut(s)	1462
AluBI	AGCT	8 cut(s)	80, 217, 799, 827, 886, 953, 1105, 1112
AluI	AGCT	8 cut(s)	80, 217, 799, 827, 886, 953, 1105, 1112
Alw21I	GWGCWC	4 cut(s)	219, 1420, 1459, 1522
Alw26I	GTCTC	1 cut(s)	1151
Alw44I	GTGCAC	2 cut(s)	1455, 1518
AlwI	GGATC	5 cut(s)	170, 183, 1214, 1227, 1610
AlwNI	CAGNNNCTG	1 cut(s)	249
AoxI	GGCC	7 cut(s)	141, 307, 630, 919, 994, 1261, 1328
ApaLI	GTGCAC	2 cut(s)	1455, 1518
ApeKI	GCWGC	7 cut(s)	77, 185, 400, 517, 563, 593, 827
ApoI	RAATTY	1 cut(s)	1172
AsiGI	ACCGGT	1 cut(s)	288
Asp700I	GAANNNNTTC	1 cut(s)	1185
AspLEI	GCGC	1 cut(s)	114
AspS9I	GGNCC	5 cut(s)	252, 291, 938, 1042, 1261
AsuC2I	CCSGG	1 cut(s)	311
AsuHPI	GGTGA	3 cut(s)	439, 870, 923
AvaII	GGWCC	4 cut(s)	252, 291, 938, 1042
BaeGI	GKGCMC	3 cut(s)	316, 1459, 1522
BamHI	GGATCC	2 cut(s)	175, 1219
BanI	GGYRCC	2 cut(s)	509, 1286
BanII	GRGCYC	1 cut(s)	219
BauI	CACGAG	1 cut(s)	1611
BbsI	GAAGAC	2 cut(s)	57, 233
Bbv12I	GWGCWC	4 cut(s)	219, 1420, 1459, 1522
BbvCI	CCTCAGC	3 cut(s)	234, 882, 954
BbvI	GCAGC	7 cut(s)	89, 172, 412, 529, 550, 580, 814
BccI	CCATC	4 cut(s)	49, 418, 892, 1003
BceAI	ACGGC	4 cut(s)	211, 246, 314, 906
BciT130I	CCWGG	1 cut(s)	122
BciVI	GTATCC	2 cut(s)	655, 1137
BcnI	CCSGG	1 cut(s)	311
BcoDI	GTCTC	1 cut(s)	1151
BcuI	ACTAGT	2 cut(s)	643, 1267
BfaI	CTAG	4 cut(s)	137, 492, 644, 1268
BfmI	CTRYAG	1 cut(s)	1113
BfrI	CTTAAG	1 cut(s)	1196
BfuAI	ACCTGC	1 cut(s)	511
BfuI	GTATCC	2 cut(s)	655, 1137
BglI	GCCNNNNNGGC	1 cut(s)	140
Bme1390I	CCNGG	2 cut(s)	122, 311
Bme18I	GGWCC	4 cut(s)	252, 291, 938, 1042
BmgT120I	GGNCC	5 cut(s)	252, 291, 938, 1042, 1261
BmiI	GGNNCC	5 cut(s)	34, 177, 511, 1221, 1288
BmrFI	CCNGG	2 cut(s)	122, 311
BmrI	ACTGGG	1 cut(s)	868
BmsI	GCATC	1 cut(s)	580
BmuI	ACTGGG	1 cut(s)	868
BoxI	GACNNNNGTC	2 cut(s)	243, 257
BpiI	GAAGAC	2 cut(s)	57, 233
BpmI	CTGGAG	2 cut(s)	1434, 1571
Bpu10I	CCTNAGC	3 cut(s)	234, 882, 954
BpuMI	CCSGG	1 cut(s)	311
Bsa29I	ATCGAT	1 cut(s)	465
BsaAI	YACGTR	1 cut(s)	305
BsaBI	GATNNNNATC	2 cut(s)	902, 1013
BsaHI	GRCGYC	3 cut(s)	201, 240, 477
BsaJI	CCNNGG	3 cut(s)	144, 255, 1528
BsaWI	WCCGGW	2 cut(s)	10, 288
BsaXI	ACNNNNNCTCC	8 cut(s)	38, 44, 68, 74, 275, 305, 816, 846
Bsc4I	CCNNNNNNNGG	6 cut(s)	29, 121, 127, 424, 1529, 1532
Bse118I	RCCGGY	2 cut(s)	29, 288
Bse1I	ACTGG	3 cut(s)	254, 874, 997
Bse8I	GATNNNNATC	2 cut(s)	902, 1013
BseBI	CCWGG	1 cut(s)	122
BseCI	ATCGAT	1 cut(s)	465
BseDI	CCNNGG	3 cut(s)	144, 255, 1528
BseGI	GGATG	3 cut(s)	41, 595, 1587
BseJI	GATNNNNATC	2 cut(s)	902, 1013
BseLI	CCNNNNNNNGG	6 cut(s)	29, 121, 127, 424, 1529, 1532
BseMII	CTCAG	6 cut(s)	225, 896, 945, 1023, 1233, 1440
BseNI	ACTGG	3 cut(s)	254, 874, 997
BseRI	GAGGAG	1 cut(s)	1415
BseSI	GKGCMC	3 cut(s)	316, 1459, 1522
BseX3I	CGGCCG	2 cut(s)	141, 919
BseXI	GCAGC	7 cut(s)	89, 172, 412, 529, 550, 580, 814
BseYI	CCCAGC	1 cut(s)	440
BsgI	GTGCAG	3 cut(s)	401, 419, 1289
Bsh1236I	CGCG	2 cut(s)	146, 704
Bsh1285I	CGRYCG	2 cut(s)	144, 922
BshFI	GGCC	7 cut(s)	143, 309, 632, 921, 996, 1263, 1330
BshNI	GGYRCC	2 cut(s)	509, 1286
BshTI	ACCGGT	1 cut(s)	288
BshVI	ATCGAT	1 cut(s)	465
BsiEI	CGRYCG	2 cut(s)	144, 922
BsiHKAI	GWGCWC	4 cut(s)	219, 1420, 1459, 1522
BsiSI	CCGG	5 cut(s)	11, 30, 289, 310, 633
BslFI	GGGAC	1 cut(s)	188
BslI	CCNNNNNNNGG	6 cut(s)	29, 121, 127, 424, 1529, 1532
BsmAI	GTCTC	1 cut(s)	1151
BsmFI	GGGAC	1 cut(s)	188
BsmI	GAATGC	1 cut(s)	723
BsnI	GGCC	7 cut(s)	143, 309, 632, 921, 996, 1263, 1330
Bsp1286I	GDGCHC	5 cut(s)	219, 316, 1420, 1459, 1522
Bsp143I	GATC	5 cut(s)	175, 210, 1014, 1219, 1615
BspANI	GGCC	7 cut(s)	143, 309, 632, 921, 996, 1263, 1330
BspCNI	CTCAG	6 cut(s)	226, 895, 946, 1024, 1234, 1441
BspDI	ATCGAT	1 cut(s)	465
BspFNI	CGCG	2 cut(s)	146, 704
BspLI	GGNNCC	5 cut(s)	34, 177, 511, 1221, 1288
BspMI	ACCTGC	1 cut(s)	511
BspPI	GGATC	5 cut(s)	170, 183, 1214, 1227, 1610
BspT107I	GGYRCC	2 cut(s)	509, 1286
BspTI	CTTAAG	1 cut(s)	1196
BsrFI	RCCGGY	2 cut(s)	29, 288
BsrI	ACTGG	3 cut(s)	254, 874, 997
BssAI	RCCGGY	2 cut(s)	29, 288
BssECI	CCNNGG	3 cut(s)	144, 255, 1528
BssMI	GATC	5 cut(s)	175, 210, 1014, 1219, 1615
BssNI	GRCGYC	3 cut(s)	201, 240, 477
BssSI	CACGAG	1 cut(s)	1611
Bst2BI	CACGAG	1 cut(s)	1611
Bst2UI	CCWGG	1 cut(s)	122
Bst4CI	ACNGT	7 cut(s)	379, 397, 412, 1094, 1370, 1517, 1532
BstACI	GRCGYC	3 cut(s)	201, 240, 477
BstAFI	CTTAAG	1 cut(s)	1196
BstBAI	YACGTR	1 cut(s)	305
BstC8I	GCNNGC	5 cut(s)	31, 630, 865, 1455, 1629
BstDEI	CTNAG	8 cut(s)	234, 882, 954, 1032, 1242, 1449, 1622, 1635
BstDSI	CCRYGG	2 cut(s)	144, 1528
BstF5I	GGATG	3 cut(s)	41, 595, 1587
BstFNI	CGCG	2 cut(s)	146, 704
BstHHI	GCGC	1 cut(s)	114
BstKTI	GATC	5 cut(s)	178, 213, 1017, 1222, 1618
BstMAI	GTCTC	1 cut(s)	1151
BstMBI	GATC	5 cut(s)	175, 210, 1014, 1219, 1615
BstMCI	CGRYCG	2 cut(s)	144, 922
BstMWI	GCNNNNNNNGC	5 cut(s)	140, 146, 629, 869, 892
BstNI	CCWGG	1 cut(s)	122
BstPAI	GACNNNNGTC	2 cut(s)	243, 257
BstSCI	CCNGG	2 cut(s)	120, 309
BstSFI	CTRYAG	1 cut(s)	1113
BstSLI	GKGCMC	3 cut(s)	316, 1459, 1522
BstUI	CGCG	2 cut(s)	146, 704
BstV1I	GCAGC	7 cut(s)	89, 172, 412, 529, 550, 580, 814
BstV2I	GAAGAC	2 cut(s)	57, 233
BstX2I	RGATCY	2 cut(s)	175, 1219
BstXI	CCANNNNNNTGG	1 cut(s)	280
BstYI	RGATCY	2 cut(s)	175, 1219
BstZI	CGGCCG	2 cut(s)	141, 919
Bsu15I	ATCGAT	1 cut(s)	465
BsuI	GTATCC	2 cut(s)	655, 1137
BsuRI	GGCC	7 cut(s)	143, 309, 632, 921, 996, 1263, 1330
BsuTUI	ATCGAT	1 cut(s)	465
BtgI	CCRYGG	2 cut(s)	144, 1528
BtgZI	GCGATG	2 cut(s)	421, 1082
BtsCI	GGATG	3 cut(s)	41, 595, 1587
BtsI	GCAGTG	1 cut(s)	389
BtsIMutI	CAGTG	4 cut(s)	247, 389, 990, 1239
BveI	ACCTGC	1 cut(s)	511
Cac8I	GCNNGC	5 cut(s)	31, 630, 865, 1455, 1629
CaiI	CAGNNNCTG	1 cut(s)	249
CciNI	GCGGCCGC	1 cut(s)	141
CfoI	GCGC	1 cut(s)	114
Cfr10I	RCCGGY	2 cut(s)	29, 288
Cfr13I	GGNCC	5 cut(s)	252, 291, 938, 1042, 1261
Cfr42I	CCGCGG	1 cut(s)	147
ClaI	ATCGAT	1 cut(s)	465
CpoI	CGGWCCG	1 cut(s)	291
CseI	GACGC	2 cut(s)	466, 1511
Csp6I	GTAC	1 cut(s)	433
CspAI	ACCGGT	1 cut(s)	288
CspI	CGGWCCG	1 cut(s)	291
CviAII	CATG	4 cut(s)	1039, 1352, 1430, 1564
CviQI	GTAC	1 cut(s)	433
DdeI	CTNAG	8 cut(s)	234, 882, 954, 1032, 1242, 1449, 1622, 1635
DpnI	GATC	5 cut(s)	177, 212, 1016, 1221, 1617
DpnII	GATC	5 cut(s)	175, 210, 1014, 1219, 1615
DraI	TTTAAA	2 cut(s)	1170, 1236
DraIII	CACNNNGTG	1 cut(s)	1424
EaeI	YGGCCR	3 cut(s)	141, 307, 919
EagI	CGGCCG	2 cut(s)	141, 919
EciI	GGCGGA	1 cut(s)	46
Ecl136II	GAGCTC	1 cut(s)	217
EclXI	CGGCCG	2 cut(s)	141, 919
Eco24I	GRGCYC	1 cut(s)	219
Eco32I	GATATC	1 cut(s)	765
Eco47I	GGWCC	4 cut(s)	252, 291, 938, 1042
Eco52I	CGGCCG	2 cut(s)	141, 919
Eco53kI	GAGCTC	1 cut(s)	217
EcoICRI	GAGCTC	1 cut(s)	217
EcoRII	CCWGG	1 cut(s)	120
EcoRV	GATATC	1 cut(s)	765
EcoT38I	GRGCYC	1 cut(s)	219
FaeI	CATG	4 cut(s)	1042, 1355, 1433, 1567
FaqI	GGGAC	1 cut(s)	188
FatI	CATG	4 cut(s)	1038, 1351, 1429, 1563
FauI	CCCGC	3 cut(s)	34, 674, 709
FblI	GTMKAC	2 cut(s)	5, 165
FokI	GGATG	3 cut(s)	28, 602, 1574
FriOI	GRGCYC	1 cut(s)	219
FspBI	CTAG	4 cut(s)	137, 492, 644, 1268
GlaI	GCGC	1 cut(s)	113
GsaI	CCCAGC	1 cut(s)	444
GsuI	CTGGAG	2 cut(s)	1434, 1571
HaeIII	GGCC	7 cut(s)	143, 309, 632, 921, 996, 1263, 1330
HapII	CCGG	5 cut(s)	11, 30, 289, 310, 633
HgaI	GACGC	2 cut(s)	466, 1511
HhaI	GCGC	1 cut(s)	114
Hin1I	GRCGYC	3 cut(s)	201, 240, 477
Hin1II	CATG	4 cut(s)	1042, 1355, 1433, 1567
Hin6I	GCGC	1 cut(s)	112
HinP1I	GCGC	1 cut(s)	112
HincII	GTYRAC	2 cut(s)	6, 166
HindII	GTYRAC	2 cut(s)	6, 166
HpaII	CCGG	5 cut(s)	11, 30, 289, 310, 633
HphI	GGTGA	3 cut(s)	439, 870, 923
Hpy166II	GTNNAC	6 cut(s)	6, 166, 375, 990, 1457, 1520
Hpy188I	TCNGA	8 cut(s)	37, 263, 362, 600, 729, 769, 1033, 1511
Hpy8I	GTNNAC	6 cut(s)	6, 166, 375, 990, 1457, 1520
Hpy99I	CGWCG	7 cut(s)	167, 170, 203, 335, 397, 482, 1505
HpyAV	CCTTC	4 cut(s)	467, 764, 812, 1175
HpyCH4III	ACNGT	7 cut(s)	379, 397, 412, 1094, 1370, 1517, 1532
HpyCH4IV	ACGT	3 cut(s)	201, 240, 304
HpyCH4V	TGCA	7 cut(s)	382, 400, 628, 1097, 1306, 1457, 1520
HpyF10VI	GCNNNNNNNGC	5 cut(s)	140, 146, 629, 869, 892
HpyF3I	CTNAG	8 cut(s)	234, 882, 954, 1032, 1242, 1449, 1622, 1635
HpySE526I	ACGT	3 cut(s)	201, 240, 304
Hsp92I	GRCGYC	3 cut(s)	201, 240, 477
Hsp92II	CATG	4 cut(s)	1042, 1355, 1433, 1567
HspAI	GCGC	1 cut(s)	112
KroI	GCCGGC	1 cut(s)	29
KroNI	GCCGGC	1 cut(s)	31
KspI	CCGCGG	1 cut(s)	147
Kzo9I	GATC	5 cut(s)	175, 210, 1014, 1219, 1615
LmnI	GCTCC	6 cut(s)	38, 124, 222, 824, 1415, 1552
Lsp1109I	GCAGC	7 cut(s)	89, 172, 412, 529, 550, 580, 814
LweI	GCATC	1 cut(s)	580
MaeI	CTAG	4 cut(s)	137, 492, 644, 1268
MaeII	ACGT	3 cut(s)	201, 240, 304
MaeIII	GTNAC	3 cut(s)	245, 876, 911
MalI	GATC	5 cut(s)	177, 212, 1016, 1221, 1617
MboI	GATC	5 cut(s)	175, 210, 1014, 1219, 1615
MboII	GAAGA	4 cut(s)	62, 238, 349, 1190
MflI	RGATCY	2 cut(s)	175, 1219
MhlI	GDGCHC	5 cut(s)	219, 316, 1420, 1459, 1522
MluCI	AATT	5 cut(s)	1172, 1256, 1372, 1407, 1470
MlyI	GAGTC	6 cut(s)	47, 464, 739, 834, 953, 1070
MmeI	TCCRAC	4 cut(s)	69, 241, 261, 1159
MroNI	GCCGGC	1 cut(s)	29
MroXI	GAANNNNTTC	1 cut(s)	1185
MseI	TTAA	6 cut(s)	504, 579, 654, 1169, 1197, 1235
MslI	CAYNNNNRTG	2 cut(s)	981, 1462
MspA1I	CMGCKG	1 cut(s)	146
MspCI	CTTAAG	1 cut(s)	1196
MspI	CCGG	5 cut(s)	11, 30, 289, 310, 633
MspR9I	CCNGG	2 cut(s)	122, 311
Mva1269I	GAATGC	1 cut(s)	723
MvaI	CCWGG	1 cut(s)	122
MvnI	CGCG	2 cut(s)	146, 704
MwoI	GCNNNNNNNGC	5 cut(s)	140, 146, 629, 869, 892
NaeI	GCCGGC	1 cut(s)	31
NciI	CCSGG	1 cut(s)	311
NdeII	GATC	5 cut(s)	175, 210, 1014, 1219, 1615
NgoMIV	GCCGGC	1 cut(s)	29
NlaIII	CATG	4 cut(s)	1042, 1355, 1433, 1567
NlaIV	GGNNCC	5 cut(s)	34, 177, 511, 1221, 1288
NmuCI	GTSAC	3 cut(s)	245, 876, 911
NotI	GCGGCCGC	1 cut(s)	141
OliI	CACNNNNGTG	1 cut(s)	1462
PaqCI	CACCTGC	1 cut(s)	511
PctI	GAATGC	1 cut(s)	723
PdiI	GCCGGC	1 cut(s)	31
PdmI	GAANNNNTTC	1 cut(s)	1185
PfeI	GAWTC	4 cut(s)	14, 322, 706, 1202
PinAI	ACCGGT	1 cut(s)	288
PleI	GAGTC	6 cut(s)	47, 463, 738, 834, 953, 1070
PpsI	GAGTC	6 cut(s)	47, 463, 738, 834, 953, 1070
Ppu21I	YACGTR	1 cut(s)	305
PshAI	GACNNNNGTC	2 cut(s)	243, 257
Psp124BI	GAGCTC	1 cut(s)	219
Psp6I	CCWGG	1 cut(s)	120
PspFI	CCCAGC	1 cut(s)	440
PspGI	CCWGG	1 cut(s)	120
PspN4I	GGNNCC	5 cut(s)	34, 177, 511, 1221, 1288
PspPI	GGNCC	5 cut(s)	252, 291, 938, 1042, 1261
PstNI	CAGNNNCTG	1 cut(s)	249
PsuI	RGATCY	2 cut(s)	175, 1219
RsaI	GTAC	1 cut(s)	434
RsaNI	GTAC	1 cut(s)	433
RseI	CAYNNNNRTG	2 cut(s)	981, 1462
Rsr2I	CGGWCCG	1 cut(s)	291
RsrII	CGGWCCG	1 cut(s)	291
SacI	GAGCTC	1 cut(s)	219
SacII	CCGCGG	1 cut(s)	147
SalI	GTCGAC	2 cut(s)	4, 164
SaqAI	TTAA	6 cut(s)	504, 579, 654, 1169, 1197, 1235
Sau3AI	GATC	5 cut(s)	175, 210, 1014, 1219, 1615
Sau96I	GGNCC	5 cut(s)	252, 291, 938, 1042, 1261
SchI	GAGTC	6 cut(s)	47, 464, 739, 834, 953, 1070
ScrFI	CCNGG	2 cut(s)	122, 311
SduI	GDGCHC	5 cut(s)	219, 316, 1420, 1459, 1522
SfaNI	GCATC	1 cut(s)	580
SfcI	CTRYAG	1 cut(s)	1113
Sfr303I	CCGCGG	1 cut(s)	147
SgrBI	CCGCGG	1 cut(s)	147
SgrDI	CGTCGACG	1 cut(s)	164
SinI	GGWCC	4 cut(s)	252, 291, 938, 1042
SmiMI	CAYNNNNRTG	2 cut(s)	981, 1462
SmlI	CTYRAG	1 cut(s)	1196
SmoI	CTYRAG	1 cut(s)	1196
SpeI	ACTAGT	2 cut(s)	643, 1267
Sse9I	AATT	5 cut(s)	1172, 1256, 1372, 1407, 1470
SspMI	CTAG	4 cut(s)	137, 492, 644, 1268
SstI	GAGCTC	1 cut(s)	219
StyD4I	CCNGG	2 cut(s)	120, 309
TaaI	ACNGT	7 cut(s)	379, 397, 412, 1094, 1370, 1517, 1532
TaiI	ACGT	3 cut(s)	204, 243, 307
TaqII	GACCGA	1 cut(s)	269
TasI	AATT	5 cut(s)	1172, 1256, 1372, 1407, 1470
TatI	WGTACW	1 cut(s)	432
TauI	GCSGC	7 cut(s)	95, 117, 120, 143, 146, 149, 420
TfiI	GAWTC	4 cut(s)	14, 322, 706, 1202
Tru1I	TTAA	6 cut(s)	504, 579, 654, 1169, 1197, 1235
Tru9I	TTAA	6 cut(s)	504, 579, 654, 1169, 1197, 1235
TscAI	CASTG	4 cut(s)	254, 389, 997, 1246
TseFI	GTSAC	3 cut(s)	245, 876, 911
TseI	GCWGC	7 cut(s)	77, 185, 400, 517, 563, 593, 827
Tsp45I	GTSAC	3 cut(s)	245, 876, 911
TspGWI	ACGGA	3 cut(s)	52, 151, 283
TspRI	CASTG	4 cut(s)	254, 389, 997, 1246
Vha464I	CTTAAG	1 cut(s)	1196
VneI	GTGCAC	2 cut(s)	1455, 1518
VpaK11BI	GGWCC	4 cut(s)	252, 291, 938, 1042
XapI	RAATTY	1 cut(s)	1172
XcmI	CCANNNNNNNNNTGG	2 cut(s)	520, 1535
XmiI	GTMKAC	2 cut(s)	5, 165
XmnI	GAANNNNTTC	1 cut(s)	1185
XspI	CTAG	4 cut(s)	137, 492, 644, 1268
ZraI	GACGTC	2 cut(s)	202, 241

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.