Rmu_sc0005375.1_g000002

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005375.1
OG0001714
Physical Location & Seq
Forward (+)
2210 .. 4804
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005375.1_g000002.1.cds

Sequence Viewer

Length: 819 bp
atgttagaatctcagccagccacccagcccaaccacgagccagagaacgaggaatcagcatctctgagcaaaaaggtggcgaagaaagaagaggctaagctcaaaaagctccgtcgcaaccaagagcaagaagcggcaccgccgcggctagcgctcgcgctcagtctgccgagccagaggacccgctcgccgctaactacggtgacgtgccgctcattcagcattgaaggaccagaaggttctgatacgcggtcatgcacagacgatctgtgccttctggtcttcgggaagaacatggcgtttaattgtgccggcgattgtgagccgtcagatggtgaagtacgtggtggcgttgagccctgggaatcgactattgtcattcaagggattgtgtctgttcctgttatggcaattaaaggcgcttcacagcaggataaacgcttgaactaccgagtactgagctccaagagggcgcccctccttttctttgttccagcagcttcaacactttgtatctgtgccattgtaggagagcgccgcccatctaggtctcttcagtcaaggctcatgccctatctgacagcacgcccttgcggcgtcgtcgtcagaagggctaacagggacctcgtgtccagtgactcgtccagtcgatggaatggcagcgtgggagatgcttcgctggttagaacgcttggggttttttccagggagagtaaggggtggctggtggtgtggagctcggagaatagtacatggaccgattgggtggcgcgtgatacgacgtcgctggatgtccataggcgtgtggcggcattgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

272

Amino Acids

29.86

Weight (kDa)

9.09

Isoelectric Point (pI)

66.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 785
AccB1I GGYRCC 2 cut(s) 136, 472
AccB7I CCANNNNNTGG 1 cut(s) 651
AccBSI CCGCTC 2 cut(s) 186, 213
AccII CGCG 4 cut(s) 145, 158, 250, 772
AcuI CTGAAG 1 cut(s) 539
AcyI GRCGYC 3 cut(s) 473, 597, 782
AfaI GTAC 3 cut(s) 342, 456, 751
AfeI AGCGCT 1 cut(s) 153
AfiI CCNNNNNNNGG 2 cut(s) 332, 651
AgsI TTSAA 4 cut(s) 227, 383, 445, 504
AhdI GACNNNNNGTC 1 cut(s) 628
AjiI CACGTC 1 cut(s) 207
AjnI CCWGG 2 cut(s) 359, 704
AluBI AGCT 5 cut(s) 100, 109, 462, 500, 738
AluI AGCT 5 cut(s) 100, 109, 462, 500, 738
Alw21I GWGCWC 2 cut(s) 464, 740
Alw26I GTCTC 1 cut(s) 555
Aor51HI AGCGCT 1 cut(s) 153
ApeKI GCWGC 2 cut(s) 497, 660
AspLEI GCGC 6 cut(s) 154, 160, 422, 475, 537, 772
AspS9I GGNCC 4 cut(s) 180, 230, 622, 756
AsuHPI GGTGA 2 cut(s) 214, 347
AsuNHI GCTAGC 1 cut(s) 148
AvaII GGWCC 4 cut(s) 180, 230, 622, 756
BanI GGYRCC 2 cut(s) 136, 472
BanII GRGCYC 3 cut(s) 360, 464, 740
BauI CACGAG 2 cut(s) 35, 626
BbsI GAAGAC 1 cut(s) 274
Bbv12I GWGCWC 2 cut(s) 464, 740
BbvI GCAGC 2 cut(s) 509, 672
BccI CCATC 3 cut(s) 326, 550, 645
BceAI ACGGC 1 cut(s) 310
BciT130I CCWGG 2 cut(s) 361, 706
BcoDI GTCTC 1 cut(s) 555
BfaI CTAG 2 cut(s) 149, 546
BfoI RGCGCY 4 cut(s) 155, 423, 476, 538
BglI GCCNNNNNGGC 1 cut(s) 594
BlpI GCTNAGC 1 cut(s) 96
BmcAI AGTACT 1 cut(s) 456
Bme1390I CCNGG 2 cut(s) 361, 706
Bme18I GGWCC 4 cut(s) 180, 230, 622, 756
BmeRI GACNNNNNGTC 1 cut(s) 628
BmgBI CACGTC 1 cut(s) 207
BmgT120I GGNCC 4 cut(s) 180, 230, 622, 756
BmiI GGNNCC 4 cut(s) 138, 182, 474, 623
BmrFI CCNGG 2 cut(s) 361, 706
BmsI GCATC 2 cut(s) 68, 661
BmtI GCTAGC 1 cut(s) 152
BoxI GACNNNNGTC 1 cut(s) 374
BpiI GAAGAC 1 cut(s) 274
Bpu1102I GCTNAGC 1 cut(s) 96
BsaAI YACGTR 1 cut(s) 344
BsaHI GRCGYC 3 cut(s) 473, 597, 782
BsaI GGTCTC 1 cut(s) 555
BsaJI CCNNGG 4 cut(s) 143, 359, 360, 705
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 651
Bse118I RCCGGY 1 cut(s) 311
Bse1I ACTGG 2 cut(s) 633, 645
BseBI CCWGG 2 cut(s) 361, 706
BseDI CCNNGG 4 cut(s) 143, 359, 360, 705
BseGI GGATG 1 cut(s) 796
BseLI CCNNNNNNNGG 2 cut(s) 332, 651
BseMII CTCAG 4 cut(s) 26, 56, 175, 449
BseNI ACTGG 2 cut(s) 633, 645
BseXI GCAGC 2 cut(s) 509, 672
BseYI CCCAGC 1 cut(s) 24
Bsh1236I CGCG 4 cut(s) 145, 158, 250, 772
BshNI GGYRCC 2 cut(s) 136, 472
BsiHKAI GWGCWC 2 cut(s) 464, 740
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 1 cut(s) 635
BslI CCNNNNNNNGG 2 cut(s) 332, 651
BsmAI GTCTC 1 cut(s) 555
BsmFI GGGAC 1 cut(s) 635
Bso31I GGTCTC 1 cut(s) 555
Bsp1286I GDGCHC 3 cut(s) 360, 464, 740
Bsp143I GATC 1 cut(s) 265
Bsp1720I GCTNAGC 1 cut(s) 96
BspCNI CTCAG 4 cut(s) 25, 57, 174, 450
BspFNI CGCG 4 cut(s) 145, 158, 250, 772
BspLI GGNNCC 4 cut(s) 138, 182, 474, 623
BspOI GCTAGC 1 cut(s) 152
BspT107I GGYRCC 2 cut(s) 136, 472
BspTNI GGTCTC 1 cut(s) 555
BsrBI CCGCTC 2 cut(s) 186, 213
BsrFI RCCGGY 1 cut(s) 311
BsrI ACTGG 2 cut(s) 633, 645
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 4 cut(s) 143, 359, 360, 705
BssMI GATC 1 cut(s) 265
BssNI GRCGYC 3 cut(s) 473, 597, 782
BssSI CACGAG 2 cut(s) 35, 626
Bst2BI CACGAG 2 cut(s) 35, 626
Bst2UI CCWGG 2 cut(s) 361, 706
Bst4CI ACNGT 1 cut(s) 202
Bst6I CTCTTC 2 cut(s) 84, 558
BstACI GRCGYC 3 cut(s) 473, 597, 782
BstBAI YACGTR 1 cut(s) 344
BstC8I GCNNGC 6 cut(s) 18, 150, 156, 188, 313, 586
BstDEI CTNAG 5 cut(s) 12, 65, 96, 161, 458
BstDSI CCRYGG 1 cut(s) 143
BstF5I GGATG 1 cut(s) 796
BstFNI CGCG 4 cut(s) 145, 158, 250, 772
BstH2I RGCGCY 4 cut(s) 155, 423, 476, 538
BstHHI GCGC 6 cut(s) 154, 160, 422, 475, 537, 772
BstKTI GATC 1 cut(s) 268
BstMAI GTCTC 1 cut(s) 555
BstMBI GATC 1 cut(s) 265
BstMWI GCNNNNNNNGC 5 cut(s) 106, 151, 166, 219, 594
BstNI CCWGG 2 cut(s) 361, 706
BstPAI GACNNNNGTC 1 cut(s) 374
BstSCI CCNGG 2 cut(s) 359, 704
BstUI CGCG 4 cut(s) 145, 158, 250, 772
BstV1I GCAGC 2 cut(s) 509, 672
BstV2I GAAGAC 1 cut(s) 274
BtgI CCRYGG 1 cut(s) 143
BtrI CACGTC 1 cut(s) 207
BtsCI GGATG 1 cut(s) 796
BtsIMutI CAGTG 1 cut(s) 640
Cac8I GCNNGC 6 cut(s) 18, 150, 156, 188, 313, 586
CfoI GCGC 6 cut(s) 154, 160, 422, 475, 537, 772
Cfr10I RCCGGY 1 cut(s) 311
Cfr13I GGNCC 4 cut(s) 180, 230, 622, 756
Cfr42I CCGCGG 1 cut(s) 146
CseI GACGC 1 cut(s) 586
Csp6I GTAC 3 cut(s) 341, 455, 750
CviAII CATG 4 cut(s) 255, 295, 568, 753
CviQI GTAC 3 cut(s) 341, 455, 750
DdeI CTNAG 5 cut(s) 12, 65, 96, 161, 458
DinI GGCGCC 1 cut(s) 474
DpnI GATC 1 cut(s) 267
DpnII GATC 1 cut(s) 265
DriI GACNNNNNGTC 1 cut(s) 628
Eam1104I CTCTTC 2 cut(s) 84, 558
Eam1105I GACNNNNNGTC 1 cut(s) 628
EarI CTCTTC 2 cut(s) 84, 558
Ecl136II GAGCTC 2 cut(s) 462, 738
Eco24I GRGCYC 3 cut(s) 360, 464, 740
Eco31I GGTCTC 1 cut(s) 555
Eco47I GGWCC 4 cut(s) 180, 230, 622, 756
Eco47III AGCGCT 1 cut(s) 153
Eco53kI GAGCTC 2 cut(s) 462, 738
Eco57I CTGAAG 1 cut(s) 539
EcoICRI GAGCTC 2 cut(s) 462, 738
EcoO109I RGGNCCY 2 cut(s) 180, 622
EcoRII CCWGG 2 cut(s) 359, 704
EcoT38I GRGCYC 3 cut(s) 360, 464, 740
EgeI GGCGCC 1 cut(s) 474
EheI GGCGCC 1 cut(s) 474
FaeI CATG 4 cut(s) 258, 298, 571, 756
FaiI YATR 6 cut(s) 256, 296, 407, 569, 754, 798
FaqI GGGAC 1 cut(s) 635
FatI CATG 4 cut(s) 254, 294, 567, 752
FauI CCCGC 1 cut(s) 191
FokI GGATG 1 cut(s) 803
FriOI GRGCYC 3 cut(s) 360, 464, 740
FspBI CTAG 2 cut(s) 149, 546
GlaI GCGC 6 cut(s) 153, 159, 421, 474, 536, 771
GsaI CCCAGC 1 cut(s) 28
HaeII RGCGCY 4 cut(s) 155, 423, 476, 538
HapII CCGG 1 cut(s) 312
HgaI GACGC 1 cut(s) 586
HhaI GCGC 6 cut(s) 154, 160, 422, 475, 537, 772
Hin1I GRCGYC 3 cut(s) 473, 597, 782
Hin1II CATG 4 cut(s) 258, 298, 571, 756
Hin6I GCGC 6 cut(s) 152, 158, 420, 473, 535, 770
HinP1I GCGC 6 cut(s) 152, 158, 420, 473, 535, 770
HinfI GANTC 4 cut(s) 8, 53, 365, 638
HpaII CCGG 1 cut(s) 312
HphI GGTGA 2 cut(s) 214, 347
Hpy188I TCNGA 6 cut(s) 66, 244, 331, 579, 608, 742
Hpy188III TCNNGA 1 cut(s) 286
Hpy99I CGWCG 5 cut(s) 117, 602, 605, 784, 787
HpyAV CCTTC 4 cut(s) 221, 230, 284, 603
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4IV ACGT 3 cut(s) 206, 343, 782
HpyCH4V TGCA 1 cut(s) 258
HpyF10VI GCNNNNNNNGC 5 cut(s) 106, 151, 166, 219, 594
HpyF3I CTNAG 5 cut(s) 12, 65, 96, 161, 458
HpySE526I ACGT 3 cut(s) 206, 343, 782
Hsp92I GRCGYC 3 cut(s) 473, 597, 782
Hsp92II CATG 4 cut(s) 258, 298, 571, 756
HspAI GCGC 6 cut(s) 152, 158, 420, 473, 535, 770
KasI GGCGCC 1 cut(s) 472
KroI GCCGGC 1 cut(s) 311
KroNI GCCGGC 1 cut(s) 313
KspI CCGCGG 1 cut(s) 146
Kzo9I GATC 1 cut(s) 265
LmnI GCTCC 3 cut(s) 114, 467, 735
Lsp1109I GCAGC 2 cut(s) 509, 672
LweI GCATC 2 cut(s) 68, 661
MaeI CTAG 2 cut(s) 149, 546
MaeII ACGT 3 cut(s) 206, 343, 782
MaeIII GTNAC 2 cut(s) 202, 635
MalI GATC 1 cut(s) 267
MbiI CCGCTC 2 cut(s) 186, 213
MboI GATC 1 cut(s) 265
MboII GAAGA 5 cut(s) 94, 101, 274, 301, 545
MhlI GDGCHC 3 cut(s) 360, 464, 740
MluCI AATT 2 cut(s) 304, 411
Mly113I GGCGCC 1 cut(s) 473
MlyI GAGTC 1 cut(s) 632
MnlI CCTC 6 cut(s) 43, 85, 171, 462, 488, 635
MroNI GCCGGC 1 cut(s) 311
MseI TTAA 2 cut(s) 303, 414
MslI CAYNNNNRTG 1 cut(s) 801
MspA1I CMGCKG 1 cut(s) 145
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 2 cut(s) 361, 706
MvaI CCWGG 2 cut(s) 361, 706
MvnI CGCG 4 cut(s) 145, 158, 250, 772
MwoI GCNNNNNNNGC 5 cut(s) 106, 151, 166, 219, 594
NaeI GCCGGC 1 cut(s) 313
NarI GGCGCC 1 cut(s) 473
NdeII GATC 1 cut(s) 265
NgoMIV GCCGGC 1 cut(s) 311
NheI GCTAGC 1 cut(s) 148
NlaIII CATG 4 cut(s) 258, 298, 571, 756
NlaIV GGNNCC 4 cut(s) 138, 182, 474, 623
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 2 cut(s) 202, 635
PasI CCCWGGG 1 cut(s) 360
PdiI GCCGGC 1 cut(s) 313
PfeI GAWTC 3 cut(s) 8, 53, 365
PflMI CCANNNNNTGG 1 cut(s) 651
PleI GAGTC 1 cut(s) 632
PluTI GGCGCC 1 cut(s) 476
PpsI GAGTC 1 cut(s) 632
Ppu21I YACGTR 1 cut(s) 344
PpuMI RGGWCCY 2 cut(s) 180, 622
PshAI GACNNNNGTC 1 cut(s) 374
Psp124BI GAGCTC 2 cut(s) 464, 740
Psp5II RGGWCCY 2 cut(s) 180, 622
Psp6I CCWGG 2 cut(s) 359, 704
PspFI CCCAGC 1 cut(s) 24
PspGI CCWGG 2 cut(s) 359, 704
PspN4I GGNNCC 4 cut(s) 138, 182, 474, 623
PspPI GGNCC 4 cut(s) 180, 230, 622, 756
PspPPI RGGWCCY 2 cut(s) 180, 622
RsaI GTAC 3 cut(s) 342, 456, 751
RsaNI GTAC 3 cut(s) 341, 455, 750
RseI CAYNNNNRTG 1 cut(s) 801
SacI GAGCTC 2 cut(s) 464, 740
SacII CCGCGG 1 cut(s) 146
SaqAI TTAA 2 cut(s) 303, 414
Sau3AI GATC 1 cut(s) 265
Sau96I GGNCC 4 cut(s) 180, 230, 622, 756
ScaI AGTACT 1 cut(s) 456
SchI GAGTC 1 cut(s) 632
ScrFI CCNGG 2 cut(s) 361, 706
SduI GDGCHC 3 cut(s) 360, 464, 740
SfaNI GCATC 2 cut(s) 68, 661
SfoI GGCGCC 1 cut(s) 474
Sfr303I CCGCGG 1 cut(s) 146
SgrBI CCGCGG 1 cut(s) 146
SinI GGWCC 4 cut(s) 180, 230, 622, 756
SmiMI CAYNNNNRTG 1 cut(s) 801
Sse9I AATT 2 cut(s) 304, 411
SspDI GGCGCC 1 cut(s) 472
SspMI CTAG 2 cut(s) 149, 546
SstI GAGCTC 2 cut(s) 464, 740
StyD4I CCNGG 2 cut(s) 359, 704
TaaI ACNGT 1 cut(s) 202
TaiI ACGT 3 cut(s) 209, 346, 785
TaqI TCGA 2 cut(s) 368, 649
TaqII GACCGA 1 cut(s) 773
TasI AATT 2 cut(s) 304, 411
TatI WGTACW 2 cut(s) 454, 749
TauI GCSGC 8 cut(s) 137, 145, 148, 193, 213, 540, 597, 812
TfiI GAWTC 3 cut(s) 8, 53, 365
Tru1I TTAA 2 cut(s) 303, 414
Tru9I TTAA 2 cut(s) 303, 414
TscAI CASTG 1 cut(s) 640
TseFI GTSAC 2 cut(s) 202, 635
TseI GCWGC 2 cut(s) 497, 660
Tsp45I GTSAC 2 cut(s) 202, 635
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 1 cut(s) 640
Van91I CCANNNNNTGG 1 cut(s) 651
VpaK11BI GGWCC 4 cut(s) 180, 230, 622, 756
XspI CTAG 2 cut(s) 149, 546
ZraI GACGTC 1 cut(s) 783
ZrmI AGTACT 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.