Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0001714

Physical Location & Seq

Genomic Coordinates Reverse (-)

12792107 .. 12795601

Gene Structure 3495 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1134400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1629 bp

ATGTCGACACCGGAATCCCAAAACCCCTCCGCCTCCGACCTAACAACCGATGACGCCTCCTCTGTAAGCAAAAGCGCTGCAAAAAAAGCCGCCAAAAAAGCCGCGAAAGAAGCCAAGAAAGAAACCCCGGCCGCGGCGGCCCCCTCTCTCGCCGTCGACGAGCAGGACCCGCTCGCCTCAAACTACGGCGAAGTACCGCTGGTCGAGCTCCAATCGAAGACGGCTGAGGACGTCAGCCACTGGACCGAGGTTGGACACTTGACCAACGCGTTGGAGAACCAGTCCGTGCTGATACGTGGCCGGGCGCAGACGATTCGCGCCGTCGGGAATAAGATGTCGTTTGTTGTCGTCAGAGAGAGGGGGTTTACGGTGCAGTGCGTGGTGACGGTGCAGCCTGACACCGTGAGCCGCCAGATGGTGAAGTACGTCGCCGGGTTGAGCAGAGAGTCGATTATCGATGTTGAAGGCGTCGTGTCTGTTCCTAGTGTCGAGATTAAAGGCACCACGCAGCAGGTGGAAGTTCAAGTAAGGAAATTGTATTGTGTGAGTAGGGCTGCTGTTTTACCAATTAACATTGAGGATGCTGCTAGGAGTGATGTCGAAATCGAAAAGGCTTTGCAGGCCGGAGAAACACTTGTTCGGGTTAATCAGGATACCCGCTTGAATAACCGAGTTCTTGACTTGCGAACACCGGCGAATCAGGGTATATTCCGCATTCAGAGTCAAGTTGGAAATATCTTCAGGCAGTTCTTGATATCTGAAGGCTTTTTTGAAATCCACTCGCCAAAGCTGATTGCTGGTTCAAGTGAAGGTGGTGCTGCTGTTTTTAAACTCAATTACAAGGGTCAAGAGGCCTGCCTTGCCCAGTCACCTCAGCTTCACAAGCAGATGGCTATATGTGGTGACTTTGGCCGTATTTTCGAGATTGGTCCTGTTTTTAGAGCTGAGGACTCCTTTACCCATAGGCATTTGTGTGAGTTCACTGGTCTTGATGTTGAAATGGAGATCAAGAGGCACTATTCTGAGGTGATGGACGTTGTTGGTCCATTGTTTGTTGCAATGTTTGACTCCTTGAACAAGAACTGTGCAAAAGAGCTTGAAGCTGTGGCGAGGCAGTATCCATTCCAACCGCTAAAGTACTTGCCAGAGACGCTACGTTTAACATTTGAAGAAGGTGTTCAGATGCTCAAGGAAGCTGGGGTTGAAGTGGATCCTTTTGGGGACTTAAACACTGAGACTGAGAGAAAATTGGGCCAACTAGTTCTGGAGAAGTATGGCACCGAGTTCTACATACTGCACCGCTACCCTTTGGCTGTTAGGCCATTCTACACAATGCCTTGCCACGACAATCCCGCTTACAGTAATTCGTTTGACGTTTTTATTCGAGGCGAGGAGATTATTTCTGGAGGACAGCGTGTACACGTTCCAGAGTTATTGACTGAGCGTGCACAGGCACTGGGAATTGACGTGAAGACGATATCAACATATATGGATTCTTTCAGATACGGTGCACCTCCACATGGTGGATTCGGAGTAGGGCTGGAGCGTGTTGTCATGCTTTTTTGCGGTTTGAATAACATCCGCAAAACTTCTCTTTTCCCTCGCGATCCACTTAGGCTCGCCCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004815 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006422 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0012505 GO:0016070 GO:0016874 GO:0016875 GO:0017101 GO:0019538 GO:0019752 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045202 GO:0046483 GO:0046686 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0090304 GO:0098542 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2 tRNA_anti-codon

Protein Analysis

543

Amino Acids

60.0

Weight (kDa)

5.88

Isoelectric Point (pI)

48.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2	PF00152	216 - 536	1.6e-78	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001714)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26870 AT4G31180 AT4G31180
fragaria_vesca	FvH4_2g15870 FvH4_2g15910 FvH4_2g30550
malus_domestica	MD08G1134400.v1.1 MD12G1153800.v1.1 MD14G1143300.v1.1 MD15G1113400.v1.1
prunus_persica	Prupe.1G463800_v2.0.a1 Prupe.4G141600_v2.0.a1 Prupe.8G055000_v2.0.a1
pyrus_communis	pycom08g11340 pycom15g10170 pycom16g11710
rosa_chinensis	RchiOBHm_Chr5g0032141 RchiOBHm_Chr5g0032161 RchiOBHm_Chr6g0278871 RchiOBHm_Chr6g0312531
rosa_laevigata	RLG00000010321 RLG00000033368
rosa_multiflora	Rmu_co8427103.1_g000001 Rmu_sc0003031.1_g000023 Rmu_sc0005375.1_g000002 Rmu_sc0005375.1_g000011 Rmu_sc0008363.1_g000011 Rmu_sc0043080.1_g000001
rosa_roxburghii	Rroxscaffold_1G00048240 Rroxscaffold_1G00048270 Rroxscaffold_7G00156770
rosa_rugosa	Rorug06G0119800 Rorug06G0120000 Rorug06G0123400 Rorug06G0396100 Rorug06G0396200
rosa_samantha	Rh5DG228900 Rh6AG228300 Rh6AG513500 Rh6BG233900 Rh6BG234000 Rh6BG524200 Rh6BG526500 Rh6CG236300 Rh6CG531400 Rh6DG515600
rosa_wichuraiana	Rw6G020030 Rw6G044650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	502
AatII	GACGTC	1 cut(s)	234
Acc36I	ACCTGC	1 cut(s)	502
AccB1I	GGYRCC	2 cut(s)	500, 1277
AccB7I	CCANNNNNTGG	1 cut(s)	1523
AccBSI	CCGCTC	1 cut(s)	172
AccI	GTMKAC	2 cut(s)	5, 156
AccII	CGCG	5 cut(s)	104, 134, 269, 318, 1605
AclWI	GGATC	3 cut(s)	1205, 1218, 1601
AcoI	YGGCCR	3 cut(s)	129, 298, 910
AcuI	CTGAAG	2 cut(s)	724, 780
AcyI	GRCGYC	3 cut(s)	54, 231, 468
AdeI	CACNNNGTG	1 cut(s)	1523
AfaI	GTAC	4 cut(s)	195, 425, 1139, 1419
AfeI	AGCGCT	1 cut(s)	76
AfiI	CCNNNNNNNGG	4 cut(s)	133, 415, 1520, 1523
AflIII	ACRYGT	2 cut(s)	267, 1420
AhlI	ACTAGT	1 cut(s)	1258
AjiI	CACGTC	1 cut(s)	1468
AluBI	AGCT	7 cut(s)	208, 790, 877, 944, 1096, 1103, 1196
AluI	AGCT	7 cut(s)	208, 790, 877, 944, 1096, 1103, 1196
Alw21I	GWGCWC	3 cut(s)	210, 1450, 1513
Alw26I	GTCTC	2 cut(s)	1142, 1229
Alw44I	GTGCAC	2 cut(s)	1446, 1509
AlwI	GGATC	3 cut(s)	1205, 1218, 1601
AlwNI	CAGNNNCTG	2 cut(s)	240, 1456
Aor51HI	AGCGCT	1 cut(s)	76
AoxI	GGCC	8 cut(s)	129, 138, 298, 621, 852, 910, 1252, 1319
ApaLI	GTGCAC	2 cut(s)	1446, 1509
ApeKI	GCWGC	6 cut(s)	77, 391, 508, 554, 584, 818
Asp700I	GAANNNNTTC	1 cut(s)	1176
AspLEI	GCGC	3 cut(s)	77, 307, 320
AspS9I	GGNCC	6 cut(s)	139, 166, 243, 929, 1043, 1252
AsuC2I	CCSGG	3 cut(s)	128, 302, 433
AsuHPI	GGTGA	5 cut(s)	394, 430, 861, 914, 1039
AvaII	GGWCC	4 cut(s)	166, 243, 929, 1043
BaeGI	GKGCMC	2 cut(s)	1450, 1513
BamHI	GGATCC	1 cut(s)	1210
BanI	GGYRCC	2 cut(s)	500, 1277
BanII	GRGCYC	1 cut(s)	210
BbsI	GAAGAC	2 cut(s)	224, 1478
Bbv12I	GWGCWC	3 cut(s)	210, 1450, 1513
BbvCI	CCTCAGC	3 cut(s)	225, 873, 945
BbvI	GCAGC	6 cut(s)	64, 403, 520, 541, 571, 805
BccI	CCATC	3 cut(s)	409, 883, 1024
BceAI	ACGGC	5 cut(s)	137, 202, 237, 305, 897
BcgI	CGANNNNNNTGC	2 cut(s)	296, 330
BciVI	GTATCC	2 cut(s)	646, 1128
BcnI	CCSGG	3 cut(s)	128, 302, 433
BcoDI	GTCTC	2 cut(s)	1142, 1229
BcuI	ACTAGT	1 cut(s)	1258
BfaI	CTAG	3 cut(s)	483, 588, 1259
BfoI	RGCGCY	1 cut(s)	78
BfuAI	ACCTGC	1 cut(s)	502
BfuI	GTATCC	2 cut(s)	646, 1128
BglI	GCCNNNNNGGC	1 cut(s)	137
BmcAI	AGTACT	1 cut(s)	1139
Bme1390I	CCNGG	3 cut(s)	128, 302, 433
Bme18I	GGWCC	4 cut(s)	166, 243, 929, 1043
BmgBI	CACGTC	1 cut(s)	1468
BmgT120I	GGNCC	6 cut(s)	139, 166, 243, 929, 1043, 1252
BmiI	GGNNCC	5 cut(s)	141, 168, 502, 1212, 1279
BmrFI	CCNGG	3 cut(s)	128, 302, 433
BmrI	ACTGGG	2 cut(s)	859, 1466
BmsI	GCATC	2 cut(s)	571, 1173
BmuI	ACTGGG	2 cut(s)	859, 1466
BpiI	GAAGAC	2 cut(s)	224, 1478
BpmI	CTGGAG	3 cut(s)	1286, 1425, 1562
Bpu10I	CCTNAGC	3 cut(s)	225, 873, 945
BpuEI	CTTGAG	1 cut(s)	1172
BpuMI	CCSGG	3 cut(s)	128, 302, 433
Bsa29I	ATCGAT	1 cut(s)	456
BsaAI	YACGTR	1 cut(s)	296
BsaHI	GRCGYC	3 cut(s)	54, 231, 468
BsaJI	CCNNGG	3 cut(s)	126, 132, 246
BsaWI	WCCGGW	1 cut(s)	10
BsaXI	ACNNNNNCTCC	2 cut(s)	266, 296
Bsc4I	CCNNNNNNNGG	4 cut(s)	133, 415, 1520, 1523
Bse118I	RCCGGY	1 cut(s)	691
Bse1I	ACTGG	5 cut(s)	245, 280, 865, 988, 1461
Bse3DI	GCAATG	1 cut(s)	1065
BseCI	ATCGAT	1 cut(s)	456
BseDI	CCNNGG	3 cut(s)	126, 132, 246
BseGI	GGATG	2 cut(s)	586, 1578
BseLI	CCNNNNNNNGG	4 cut(s)	133, 415, 1520, 1523
BseMI	GCAATG	1 cut(s)	1065
BseMII	CTCAG	7 cut(s)	216, 887, 936, 1014, 1224, 1230, 1431
BseNI	ACTGG	5 cut(s)	245, 280, 865, 988, 1461
BseRI	GAGGAG	2 cut(s)	49, 1406
BseSI	GKGCMC	2 cut(s)	1450, 1513
BseX3I	CGGCCG	1 cut(s)	129
BseXI	GCAGC	6 cut(s)	64, 403, 520, 541, 571, 805
BseYI	CCCAGC	1 cut(s)	1196
BsgI	GTGCAG	3 cut(s)	392, 410, 1280
Bsh1236I	CGCG	5 cut(s)	104, 134, 269, 318, 1605
Bsh1285I	CGRYCG	1 cut(s)	132
BshFI	GGCC	8 cut(s)	131, 140, 300, 623, 854, 912, 1254, 1321
BshNI	GGYRCC	2 cut(s)	500, 1277
BshVI	ATCGAT	1 cut(s)	456
BsiEI	CGRYCG	1 cut(s)	132
BsiHKAI	GWGCWC	3 cut(s)	210, 1450, 1513
BsiSI	CCGG	6 cut(s)	11, 128, 301, 432, 624, 692
BslFI	GGGAC	1 cut(s)	1235
BslI	CCNNNNNNNGG	4 cut(s)	133, 415, 1520, 1523
BsmAI	GTCTC	2 cut(s)	1142, 1229
BsmBI	CGTCTC	1 cut(s)	1142
BsmFI	GGGAC	1 cut(s)	1235
BsmI	GAATGC	1 cut(s)	714
BsnI	GGCC	8 cut(s)	131, 140, 300, 623, 854, 912, 1254, 1321
Bsp1286I	GDGCHC	3 cut(s)	210, 1450, 1513
Bsp1407I	TGTACA	1 cut(s)	1417
Bsp143I	GATC	3 cut(s)	1005, 1210, 1606
Bsp68I	TCGCGA	1 cut(s)	1605
BspANI	GGCC	8 cut(s)	131, 140, 300, 623, 854, 912, 1254, 1321
BspCNI	CTCAG	7 cut(s)	217, 886, 937, 1015, 1225, 1231, 1432
BspDI	ATCGAT	1 cut(s)	456
BspFNI	CGCG	5 cut(s)	104, 134, 269, 318, 1605
BspLI	GGNNCC	5 cut(s)	141, 168, 502, 1212, 1279
BspMI	ACCTGC	1 cut(s)	502
BspPI	GGATC	3 cut(s)	1205, 1218, 1601
BspT107I	GGYRCC	2 cut(s)	500, 1277
BsrBI	CCGCTC	1 cut(s)	172
BsrDI	GCAATG	1 cut(s)	1065
BsrFI	RCCGGY	1 cut(s)	691
BsrGI	TGTACA	1 cut(s)	1417
BsrI	ACTGG	5 cut(s)	245, 280, 865, 988, 1461
BssAI	RCCGGY	1 cut(s)	691
BssECI	CCNNGG	3 cut(s)	126, 132, 246
BssMI	GATC	3 cut(s)	1005, 1210, 1606
BssNI	GRCGYC	3 cut(s)	54, 231, 468
Bst4CI	ACNGT	6 cut(s)	370, 388, 403, 1085, 1361, 1508
BstACI	GRCGYC	3 cut(s)	54, 231, 468
BstAUI	TGTACA	1 cut(s)	1417
BstBAI	YACGTR	1 cut(s)	296
BstC8I	GCNNGC	5 cut(s)	174, 621, 856, 1446, 1620
BstDEI	CTNAG	9 cut(s)	225, 873, 945, 1023, 1233, 1239, 1440, 1613, 1626
BstDSI	CCRYGG	1 cut(s)	132
BstF5I	GGATG	2 cut(s)	586, 1578
BstFNI	CGCG	5 cut(s)	104, 134, 269, 318, 1605
BstH2I	RGCGCY	1 cut(s)	78
BstHHI	GCGC	3 cut(s)	77, 307, 320
BstKTI	GATC	3 cut(s)	1008, 1213, 1609
BstMAI	GTCTC	2 cut(s)	1142, 1229
BstMBI	GATC	3 cut(s)	1005, 1210, 1606
BstMCI	CGRYCG	1 cut(s)	132
BstSCI	CCNGG	3 cut(s)	126, 300, 431
BstSLI	GKGCMC	2 cut(s)	1450, 1513
BstUI	CGCG	5 cut(s)	104, 134, 269, 318, 1605
BstV1I	GCAGC	6 cut(s)	64, 403, 520, 541, 571, 805
BstV2I	GAAGAC	2 cut(s)	224, 1478
BstX2I	RGATCY	1 cut(s)	1210
BstXI	CCANNNNNNTGG	1 cut(s)	271
BstYI	RGATCY	1 cut(s)	1210
BstZI	CGGCCG	1 cut(s)	129
Bsu15I	ATCGAT	1 cut(s)	456
BsuI	GTATCC	2 cut(s)	646, 1128
BsuRI	GGCC	8 cut(s)	131, 140, 300, 623, 854, 912, 1254, 1321
BsuTUI	ATCGAT	1 cut(s)	456
BtgI	CCRYGG	1 cut(s)	132
BtrI	CACGTC	1 cut(s)	1468
BtsCI	GGATG	2 cut(s)	586, 1578
BtsI	GCAGTG	1 cut(s)	380
BtsIMutI	CAGTG	5 cut(s)	238, 380, 981, 1230, 1454
BtuMI	TCGCGA	1 cut(s)	1605
BveI	ACCTGC	1 cut(s)	502
Cac8I	GCNNGC	5 cut(s)	174, 621, 856, 1446, 1620
CaiI	CAGNNNCTG	2 cut(s)	240, 1456
CfoI	GCGC	3 cut(s)	77, 307, 320
Cfr10I	RCCGGY	1 cut(s)	691
Cfr13I	GGNCC	6 cut(s)	139, 166, 243, 929, 1043, 1252
Cfr42I	CCGCGG	1 cut(s)	135
ClaI	ATCGAT	1 cut(s)	456
CseI	GACGC	3 cut(s)	62, 457, 1159
Csp6I	GTAC	4 cut(s)	194, 424, 1138, 1418
CviAII	CATG	2 cut(s)	1520, 1555
CviQI	GTAC	4 cut(s)	194, 424, 1138, 1418
DdeI	CTNAG	9 cut(s)	225, 873, 945, 1023, 1233, 1239, 1440, 1613, 1626
DpnI	GATC	3 cut(s)	1007, 1212, 1608
DpnII	GATC	3 cut(s)	1005, 1210, 1606
DraI	TTTAAA	1 cut(s)	829
DraIII	CACNNNGTG	1 cut(s)	1523
EaeI	YGGCCR	3 cut(s)	129, 298, 910
EagI	CGGCCG	1 cut(s)	129
EciI	GGCGGA	1 cut(s)	19
Ecl136II	GAGCTC	1 cut(s)	208
EclXI	CGGCCG	1 cut(s)	129
Eco147I	AGGCCT	1 cut(s)	854
Eco24I	GRGCYC	1 cut(s)	210
Eco32I	GATATC	2 cut(s)	756, 1479
Eco47I	GGWCC	4 cut(s)	166, 243, 929, 1043
Eco47III	AGCGCT	1 cut(s)	76
Eco52I	CGGCCG	1 cut(s)	129
Eco53kI	GAGCTC	1 cut(s)	208
Eco57I	CTGAAG	2 cut(s)	724, 780
EcoICRI	GAGCTC	1 cut(s)	208
EcoO109I	RGGNCCY	1 cut(s)	166
EcoRV	GATATC	2 cut(s)	756, 1479
EcoT38I	GRGCYC	1 cut(s)	210
Esp3I	CGTCTC	1 cut(s)	1142
FaeI	CATG	2 cut(s)	1523, 1558
FaqI	GGGAC	1 cut(s)	1235
FatI	CATG	2 cut(s)	1519, 1554
FauI	CCCGC	3 cut(s)	177, 665, 1360
FblI	GTMKAC	2 cut(s)	5, 156
FokI	GGATG	2 cut(s)	593, 1565
FriOI	GRGCYC	1 cut(s)	210
FspBI	CTAG	3 cut(s)	483, 588, 1259
GlaI	GCGC	3 cut(s)	76, 306, 319
GsaI	CCCAGC	1 cut(s)	1200
GsuI	CTGGAG	3 cut(s)	1286, 1425, 1562
HaeII	RGCGCY	1 cut(s)	78
HaeIII	GGCC	8 cut(s)	131, 140, 300, 623, 854, 912, 1254, 1321
HapII	CCGG	6 cut(s)	11, 128, 301, 432, 624, 692
HgaI	GACGC	3 cut(s)	62, 457, 1159
HhaI	GCGC	3 cut(s)	77, 307, 320
Hin1I	GRCGYC	3 cut(s)	54, 231, 468
Hin1II	CATG	2 cut(s)	1523, 1558
Hin6I	GCGC	3 cut(s)	75, 305, 318
HinP1I	GCGC	3 cut(s)	75, 305, 318
HincII	GTYRAC	2 cut(s)	6, 157
HindII	GTYRAC	2 cut(s)	6, 157
HinfI	GANTC	9 cut(s)	14, 313, 446, 697, 721, 950, 1067, 1493, 1527
HpaII	CCGG	6 cut(s)	11, 128, 301, 432, 624, 692
HphI	GGTGA	5 cut(s)	394, 430, 861, 914, 1039
Hpy166II	GTNNAC	8 cut(s)	6, 157, 366, 981, 1418, 1420, 1448, 1511
Hpy188I	TCNGA	8 cut(s)	37, 353, 720, 760, 1024, 1182, 1502, 1532
Hpy8I	GTNNAC	8 cut(s)	6, 157, 366, 981, 1418, 1420, 1448, 1511
Hpy99I	CGWCG	5 cut(s)	158, 161, 326, 431, 473
HpyAV	CCTTC	4 cut(s)	458, 755, 803, 1166
HpyCH4III	ACNGT	6 cut(s)	370, 388, 403, 1085, 1361, 1508
HpyCH4IV	ACGT	8 cut(s)	231, 295, 426, 1035, 1156, 1374, 1422, 1467
HpyCH4V	TGCA	9 cut(s)	80, 373, 391, 619, 1058, 1088, 1297, 1448, 1511
HpyF3I	CTNAG	9 cut(s)	225, 873, 945, 1023, 1233, 1239, 1440, 1613, 1626
HpySE526I	ACGT	8 cut(s)	231, 295, 426, 1035, 1156, 1374, 1422, 1467
Hsp92I	GRCGYC	3 cut(s)	54, 231, 468
Hsp92II	CATG	2 cut(s)	1523, 1558
HspAI	GCGC	3 cut(s)	75, 305, 318
KspI	CCGCGG	1 cut(s)	135
Kzo9I	GATC	3 cut(s)	1005, 1210, 1606
LmnI	GCTCC	2 cut(s)	213, 1543
Lsp1109I	GCAGC	6 cut(s)	64, 403, 520, 541, 571, 805
LweI	GCATC	2 cut(s)	571, 1173
MaeI	CTAG	3 cut(s)	483, 588, 1259
MaeII	ACGT	8 cut(s)	231, 295, 426, 1035, 1156, 1374, 1422, 1467
MaeIII	GTNAC	3 cut(s)	382, 867, 902
MalI	GATC	3 cut(s)	1007, 1212, 1608
MbiI	CCGCTC	1 cut(s)	172
MboI	GATC	3 cut(s)	1005, 1210, 1606
MboII	GAAGA	4 cut(s)	229, 730, 1181, 1483
MflI	RGATCY	1 cut(s)	1210
MhlI	GDGCHC	3 cut(s)	210, 1450, 1513
MluCI	AATT	6 cut(s)	533, 567, 835, 1247, 1363, 1461
MluI	ACGCGT	1 cut(s)	267
MlyI	GAGTC	4 cut(s)	455, 730, 944, 1061
MmeI	TCCRAC	5 cut(s)	60, 232, 252, 709, 1150
MroXI	GAANNNNTTC	1 cut(s)	1176
MseI	TTAA	6 cut(s)	495, 570, 645, 828, 1160, 1226
MslI	CAYNNNNRTG	1 cut(s)	972
MspA1I	CMGCKG	2 cut(s)	134, 199
MspI	CCGG	6 cut(s)	11, 128, 301, 432, 624, 692
MspR9I	CCNGG	3 cut(s)	128, 302, 433
Mva1269I	GAATGC	1 cut(s)	714
MvnI	CGCG	5 cut(s)	104, 134, 269, 318, 1605
NciI	CCSGG	3 cut(s)	128, 302, 433
NdeII	GATC	3 cut(s)	1005, 1210, 1606
NlaIII	CATG	2 cut(s)	1523, 1558
NlaIV	GGNNCC	5 cut(s)	141, 168, 502, 1212, 1279
NmuCI	GTSAC	3 cut(s)	382, 867, 902
NruI	TCGCGA	1 cut(s)	1605
PaqCI	CACCTGC	1 cut(s)	502
PceI	AGGCCT	1 cut(s)	854
PcsI	WCGNNNNNNNCGW	1 cut(s)	156
PctI	GAATGC	1 cut(s)	714
PdmI	GAANNNNTTC	1 cut(s)	1176
PfeI	GAWTC	5 cut(s)	14, 313, 697, 1493, 1527
PflMI	CCANNNNNTGG	1 cut(s)	1523
PleI	GAGTC	4 cut(s)	454, 729, 944, 1061
PpsI	GAGTC	4 cut(s)	454, 729, 944, 1061
Ppu21I	YACGTR	1 cut(s)	296
PpuMI	RGGWCCY	1 cut(s)	166
Psp124BI	GAGCTC	1 cut(s)	210
Psp5II	RGGWCCY	1 cut(s)	166
PspFI	CCCAGC	1 cut(s)	1196
PspN4I	GGNNCC	5 cut(s)	141, 168, 502, 1212, 1279
PspPI	GGNCC	6 cut(s)	139, 166, 243, 929, 1043, 1252
PspPPI	RGGWCCY	1 cut(s)	166
PstNI	CAGNNNCTG	2 cut(s)	240, 1456
PsuI	RGATCY	1 cut(s)	1210
RruI	TCGCGA	1 cut(s)	1605
RsaI	GTAC	4 cut(s)	195, 425, 1139, 1419
RsaNI	GTAC	4 cut(s)	194, 424, 1138, 1418
RseI	CAYNNNNRTG	1 cut(s)	972
SacI	GAGCTC	1 cut(s)	210
SacII	CCGCGG	1 cut(s)	135
SalI	GTCGAC	2 cut(s)	4, 155
SaqAI	TTAA	6 cut(s)	495, 570, 645, 828, 1160, 1226
Sau3AI	GATC	3 cut(s)	1005, 1210, 1606
Sau96I	GGNCC	6 cut(s)	139, 166, 243, 929, 1043, 1252
ScaI	AGTACT	1 cut(s)	1139
SchI	GAGTC	4 cut(s)	455, 730, 944, 1061
ScrFI	CCNGG	3 cut(s)	128, 302, 433
SduI	GDGCHC	3 cut(s)	210, 1450, 1513
SfaNI	GCATC	2 cut(s)	571, 1173
SfiI	GGCCNNNNNGGCC	1 cut(s)	137
Sfr303I	CCGCGG	1 cut(s)	135
SgrAI	CRCCGGYG	1 cut(s)	691
SgrBI	CCGCGG	1 cut(s)	135
SgrDI	CGTCGACG	1 cut(s)	155
SinI	GGWCC	4 cut(s)	166, 243, 929, 1043
SmiMI	CAYNNNNRTG	1 cut(s)	972
SmlI	CTYRAG	1 cut(s)	1187
SmoI	CTYRAG	1 cut(s)	1187
SpeI	ACTAGT	1 cut(s)	1258
Sse9I	AATT	6 cut(s)	533, 567, 835, 1247, 1363, 1461
SseBI	AGGCCT	1 cut(s)	854
SspMI	CTAG	3 cut(s)	483, 588, 1259
SstI	GAGCTC	1 cut(s)	210
StuI	AGGCCT	1 cut(s)	854
StyD4I	CCNGG	3 cut(s)	126, 300, 431
TaaI	ACNGT	6 cut(s)	370, 388, 403, 1085, 1361, 1508
TaiI	ACGT	8 cut(s)	234, 298, 429, 1038, 1159, 1377, 1425, 1470
TaqII	GACCGA	1 cut(s)	260
TasI	AATT	6 cut(s)	533, 567, 835, 1247, 1363, 1461
TatI	WGTACW	2 cut(s)	1137, 1417
TauI	GCSGC	6 cut(s)	92, 104, 134, 137, 140, 411
TfiI	GAWTC	5 cut(s)	14, 313, 697, 1493, 1527
Tru1I	TTAA	6 cut(s)	495, 570, 645, 828, 1160, 1226
Tru9I	TTAA	6 cut(s)	495, 570, 645, 828, 1160, 1226
TscAI	CASTG	5 cut(s)	245, 380, 988, 1237, 1461
TseFI	GTSAC	3 cut(s)	382, 867, 902
TseI	GCWGC	6 cut(s)	77, 391, 508, 554, 584, 818
Tsp45I	GTSAC	3 cut(s)	382, 867, 902
TspGWI	ACGGA	1 cut(s)	274
TspRI	CASTG	5 cut(s)	245, 380, 988, 1237, 1461
Van91I	CCANNNNNTGG	1 cut(s)	1523
VneI	GTGCAC	2 cut(s)	1446, 1509
VpaK11BI	GGWCC	4 cut(s)	166, 243, 929, 1043
XcmI	CCANNNNNNNNNTGG	1 cut(s)	511
XmiI	GTMKAC	2 cut(s)	5, 156
XmnI	GAANNNNTTC	1 cut(s)	1176
XspI	CTAG	3 cut(s)	483, 588, 1259
ZraI	GACGTC	1 cut(s)	232
ZrmI	AGTACT	1 cut(s)	1139

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.