Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0001714

Physical Location & Seq

Genomic Coordinates Forward (+)

69111500 .. 69114751

Gene Structure 3252 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ28115

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGTCAGAACCTCAGCCACCCTCCGAGCCCACCCAGGAGCCAGAGAACGAGGAGTCCGCCTCACTCAGCAAAAAGGCCGCCAAGAAAGAAGCCGCCAAGCTCGAGAAGCTCCGTCGCCGCCAGGAGCAAGAAGCTGCGGCCGCCGCGGCTCGCGCTGAGTCCGCCGAGCCGGAGGACCCACTCGCCGCGAACTACGGCGACTTGCCGCTCATTCAGCTCCAATCGATGAGGATCGCCGACGTCAACGACTGGACGGAGGTCGGAGCGCTGACGGAGGCGTTGAAGGACCAGAAGGTTCTGATACGCGGCCGCGCGCAGACGATCCGAGCCGTCGGGAAGAACATGGCGTTTATTGTCGTCAGAGAGAAGGGCTTCACGGTTCAGTGCGTCGCCACGGTGCAGCCGGAGATTGTCAGCCGTCAGATGGTCAAGTACGTGGCGGCGTTAAACCGCGAGTCCATTATTGACATTGAAGGGATTGTGTCTGTTCCTGGCGTGGCAATTAAAGGCGCTTCACAGCAGGTGGAGGTGCAAGTCAGAAAGTTGTACTGTATAAGTAAGGCGGCTGTTTTGCCTATCAACATTGAGGATGCTGCTCGAAGTGATGCCGAAATTGAAAAAGCTAATCAGGCTGGAGAACAACTGGTTCGTGTAAATCAGGATACTCGCTTGAACTTCCGAGTACTTGACTTGCGAACACCGGCTAACCAGGGGATTTTCCGAATCCAGAGTCAAGTTGGAACTATCTTCAGGCAGTTCTTGCTGTCTGAAGGCTTTTTTGAAATCCACTCACCAAAACTGATCGCTGGTTCAAGTGAAGGTGGGGCTTCCGTGTTTAAACTGGACTACAAGGGGCAACCTGCCTGCCTTGCCCAGTCACCGCAGCTCCACAAGCAGATGGCCATCTGTGGTGACTTTGGTCGTATTTTTGAGATTGGTCCTGTTTTCAGAGCTGAGGACTCCTATACACATAGGCATCTATGTGAGTTCACTGGTCTCGATATTGAGATGGAGATTAAGTGGAGCTATTTTGAGGTTATGGATATTGTTAGCAGGTTGTTTGTTACTATGTTTGACTATTTGAACAAAAATTGTGAGAAGGAACTTGAAGCTGTACGGAGGCAGTATCCCTTCGAACCACTAAAGTACTTGCCTGAGACTCTACGTCTCACTTTTGCGGAAGGTGTTCAAATGCTGAAGGATGCTGGTGTCGAAGTAGATCCTATGGGGGACCTAAATACTGAAGCAGAGAGGAAGTTGGGCCAATTAGTTTTGGAAAAATATGGCACTGAGTTCTACATACTTCACCGCTATCCGTTGGCTGTGAGGCCTTTCTATACCATGCCTTGCTGTGACAATAAGGAGTACAGTAATTCATTTGATGTCTTCATTCGAGGTGAGGAGATTATATCAGGTGCGCAACGTATCCATGTACCAGAGTTCTTGGCTGAACGTGCTGAAGCACGTGGAATTGAAGTCAAGACAATATCAACATATATTGATTCCTTCAGATATGGTGCACCTCCCCATGGTGGATTTGGAGTAGGGCTGGAGCGTGTTGTGATGCTCTTCTGTGGTTTGAATAACATCCGCAAAACATCCTTATTCCCACGTGATCCGCTCCGGCTAGCTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004815 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006422 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0012505 GO:0016070 GO:0016874 GO:0016875 GO:0017101 GO:0019538 GO:0019752 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045202 GO:0046483 GO:0046686 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0090304 GO:0098542 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2 tRNA_anti-codon

Protein Analysis

545

Amino Acids

61.17

Weight (kDa)

5.68

Isoelectric Point (pI)

48.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2	PF00152	219 - 539	1.5e-77	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001714)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26870 AT4G31180 AT4G31180
fragaria_vesca	FvH4_2g15870 FvH4_2g15910 FvH4_2g30550
malus_domestica	MD08G1134400.v1.1 MD12G1153800.v1.1 MD14G1143300.v1.1 MD15G1113400.v1.1
prunus_persica	Prupe.1G463800_v2.0.a1 Prupe.4G141600_v2.0.a1 Prupe.8G055000_v2.0.a1
pyrus_communis	pycom08g11340 pycom15g10170 pycom16g11710
rosa_chinensis	RchiOBHm_Chr5g0032141 RchiOBHm_Chr5g0032161 RchiOBHm_Chr6g0278871 RchiOBHm_Chr6g0312531
rosa_laevigata	RLG00000010321 RLG00000033368
rosa_multiflora	Rmu_co8427103.1_g000001 Rmu_sc0003031.1_g000023 Rmu_sc0005375.1_g000002 Rmu_sc0005375.1_g000011 Rmu_sc0008363.1_g000011 Rmu_sc0043080.1_g000001
rosa_roxburghii	Rroxscaffold_1G00048240 Rroxscaffold_1G00048270 Rroxscaffold_7G00156770
rosa_rugosa	Rorug06G0119800 Rorug06G0120000 Rorug06G0123400 Rorug06G0396100 Rorug06G0396200
rosa_samantha	Rh5DG228900 Rh6AG228300 Rh6AG513500 Rh6BG233900 Rh6BG234000 Rh6BG524200 Rh6BG526500 Rh6CG236300 Rh6CG531400 Rh6DG515600
rosa_wichuraiana	Rw6G020030 Rw6G044650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	511
AatII	GACGTC	1 cut(s)	243
Acc16I	TGCGCA	1 cut(s)	1419
Acc36I	ACCTGC	3 cut(s)	511, 868, 1044
AccBSI	CCGCTC	2 cut(s)	208, 1621
AccII	CGCG	7 cut(s)	146, 153, 188, 306, 312, 314, 453
AclWI	GGATC	4 cut(s)	239, 316, 1214, 1610
AcoI	YGGCCR	3 cut(s)	138, 307, 900
AcuI	CTGAAG	6 cut(s)	733, 789, 1217, 1263, 1479, 1492
AcvI	CACGTG	2 cut(s)	1466, 1613
AcyI	GRCGYC	1 cut(s)	240
AfaI	GTAC	7 cut(s)	434, 548, 684, 1116, 1148, 1367, 1434
AfeI	AGCGCT	1 cut(s)	267
AfiI	CCNNNNNNNGG	3 cut(s)	424, 1529, 1532
AhdI	GACNNNNNGTC	1 cut(s)	1164
AjnI	CCWGG	4 cut(s)	33, 120, 490, 708
AloI	GAACNNNNNNTCC	2 cut(s)	38, 70
Alw21I	GWGCWC	1 cut(s)	1522
Alw26I	GTCTC	3 cut(s)	1001, 1151, 1172
Alw44I	GTGCAC	1 cut(s)	1518
AlwI	GGATC	4 cut(s)	239, 316, 1214, 1610
Ama87I	CYCGRG	1 cut(s)	101
Aor51HI	AGCGCT	1 cut(s)	267
AoxI	GGCC	6 cut(s)	75, 138, 307, 900, 1261, 1328
ApaLI	GTGCAC	1 cut(s)	1518
ApeKI	GCWGC	4 cut(s)	134, 400, 593, 883
ArsI	GACNNNNNNTTYG	2 cut(s)	715, 747
Asp700I	GAANNNNTTC	2 cut(s)	371, 1185
AspLEI	GCGC	6 cut(s)	155, 268, 314, 316, 512, 1420
AspS9I	GGNCC	5 cut(s)	175, 286, 938, 1231, 1261
AsuHPI	GGTGA	5 cut(s)	783, 870, 923, 1298, 1409
AsuII	TTCGAA	1 cut(s)	1134
AsuNHI	GCTAGC	1 cut(s)	1627
AvaI	CYCGRG	1 cut(s)	101
AvaII	GGWCC	4 cut(s)	175, 286, 938, 1231
BaeGI	GKGCMC	1 cut(s)	1522
BalI	TGGCCA	1 cut(s)	902
BanII	GRGCYC	1 cut(s)	30
BbrPI	CACGTG	2 cut(s)	1466, 1613
BbsI	GAAGAC	1 cut(s)	1378
Bbv12I	GWGCWC	1 cut(s)	1522
BbvCI	CCTCAGC	2 cut(s)	12, 954
BbvI	GCAGC	4 cut(s)	121, 412, 580, 895
BccI	CCATC	4 cut(s)	418, 892, 911, 1003
BceAI	ACGGC	3 cut(s)	211, 314, 402
BciT130I	CCWGG	4 cut(s)	35, 122, 492, 710
BciVI	GTATCC	3 cut(s)	655, 1137, 1436
BcoDI	GTCTC	3 cut(s)	1001, 1151, 1172
BfaI	CTAG	1 cut(s)	1628
BfoI	RGCGCY	2 cut(s)	269, 513
BfuAI	ACCTGC	3 cut(s)	511, 868, 1044
BfuI	GTATCC	3 cut(s)	655, 1137, 1436
BglI	GCCNNNNNGGC	1 cut(s)	146
BmcAI	AGTACT	2 cut(s)	684, 1148
Bme1390I	CCNGG	4 cut(s)	35, 122, 492, 710
Bme18I	GGWCC	4 cut(s)	175, 286, 938, 1231
BmeRI	GACNNNNNGTC	1 cut(s)	1164
BmeT110I	CYCGRG	1 cut(s)	101
BmgT120I	GGNCC	5 cut(s)	175, 286, 938, 1231, 1261
BmiI	GGNNCC	3 cut(s)	39, 177, 1232
BmrFI	CCNGG	4 cut(s)	35, 122, 492, 710
BmrI	ACTGGG	1 cut(s)	868
BmsI	GCATC	5 cut(s)	580, 595, 985, 1192, 1554
BmtI	GCTAGC	1 cut(s)	1631
BmuI	ACTGGG	1 cut(s)	868
BoxI	GACNNNNGTC	2 cut(s)	257, 918
BpiI	GAAGAC	1 cut(s)	1378
BplI	GAGNNNNNCTC	2 cut(s)	44, 76
BpmI	CTGGAG	2 cut(s)	654, 1571
Bpu10I	CCTNAGC	2 cut(s)	12, 954
Bpu14I	TTCGAA	1 cut(s)	1134
Bsa29I	ATCGAT	1 cut(s)	224
BsaAI	YACGTR	3 cut(s)	436, 1466, 1613
BsaBI	GATNNNNATC	2 cut(s)	230, 902
BsaHI	GRCGYC	1 cut(s)	240
BsaI	GGTCTC	1 cut(s)	1001
BsaJI	CCNNGG	5 cut(s)	33, 144, 393, 709, 1528
BsaXI	ACNNNNNCTCC	4 cut(s)	870, 900, 1004, 1034
Bsc4I	CCNNNNNNNGG	3 cut(s)	424, 1529, 1532
Bse118I	RCCGGY	1 cut(s)	700
Bse1I	ACTGG	5 cut(s)	254, 648, 846, 874, 997
Bse8I	GATNNNNATC	2 cut(s)	230, 902
BseBI	CCWGG	4 cut(s)	35, 122, 492, 710
BseCI	ATCGAT	1 cut(s)	224
BseDI	CCNNGG	5 cut(s)	33, 144, 393, 709, 1528
BseGI	GGATG	4 cut(s)	595, 1207, 1587, 1598
BseJI	GATNNNNATC	2 cut(s)	230, 902
BseLI	CCNNNNNNNGG	3 cut(s)	424, 1529, 1532
BseMII	CTCAG	6 cut(s)	26, 79, 147, 945, 1146, 1281
BseNI	ACTGG	5 cut(s)	254, 648, 846, 874, 997
BsePI	GCGCGC	1 cut(s)	312
BseRI	GAGGAG	2 cut(s)	65, 1415
BseSI	GKGCMC	1 cut(s)	1522
BseX3I	CGGCCG	2 cut(s)	138, 307
BseXI	GCAGC	4 cut(s)	121, 412, 580, 895
BsgI	GTGCAG	1 cut(s)	419
Bsh1236I	CGCG	7 cut(s)	146, 153, 188, 306, 312, 314, 453
Bsh1285I	CGRYCG	2 cut(s)	141, 310
BshFI	GGCC	6 cut(s)	77, 140, 309, 902, 1263, 1330
BshVI	ATCGAT	1 cut(s)	224
BsiEI	CGRYCG	2 cut(s)	141, 310
BsiHKAI	GWGCWC	1 cut(s)	1522
BsiHKCI	CYCGRG	1 cut(s)	101
BsiSI	CCGG	4 cut(s)	170, 404, 701, 1624
BslFI	GGGAC	1 cut(s)	1244
BslI	CCNNNNNNNGG	3 cut(s)	424, 1529, 1532
BsmAI	GTCTC	3 cut(s)	1001, 1151, 1172
BsmBI	CGTCTC	1 cut(s)	1172
BsmFI	GGGAC	1 cut(s)	1244
BsnI	GGCC	6 cut(s)	77, 140, 309, 902, 1263, 1330
Bso31I	GGTCTC	1 cut(s)	1001
BsoBI	CYCGRG	1 cut(s)	101
Bsp119I	TTCGAA	1 cut(s)	1134
Bsp1286I	GDGCHC	2 cut(s)	30, 1522
Bsp143I	GATC	5 cut(s)	231, 321, 801, 1219, 1615
Bsp19I	CCATGG	1 cut(s)	1528
BspANI	GGCC	6 cut(s)	77, 140, 309, 902, 1263, 1330
BspCNI	CTCAG	6 cut(s)	25, 78, 148, 946, 1147, 1282
BspDI	ATCGAT	1 cut(s)	224
BspFNI	CGCG	7 cut(s)	146, 153, 188, 306, 312, 314, 453
BspLI	GGNNCC	3 cut(s)	39, 177, 1232
BspMI	ACCTGC	3 cut(s)	511, 868, 1044
BspOI	GCTAGC	1 cut(s)	1631
BspPI	GGATC	4 cut(s)	239, 316, 1214, 1610
BspQI	GCTCTTC	1 cut(s)	1574
BspT104I	TTCGAA	1 cut(s)	1134
BspTNI	GGTCTC	1 cut(s)	1001
BsrBI	CCGCTC	2 cut(s)	208, 1621
BsrFI	RCCGGY	1 cut(s)	700
BsrI	ACTGG	5 cut(s)	254, 648, 846, 874, 997
BssAI	RCCGGY	1 cut(s)	700
BssECI	CCNNGG	5 cut(s)	33, 144, 393, 709, 1528
BssHII	GCGCGC	1 cut(s)	312
BssMI	GATC	5 cut(s)	231, 321, 801, 1219, 1615
BssNI	GRCGYC	1 cut(s)	240
BssT1I	CCWWGG	1 cut(s)	1528
Bst2UI	CCWGG	4 cut(s)	35, 122, 492, 710
Bst4CI	ACNGT	4 cut(s)	379, 397, 551, 1370
Bst6I	CTCTTC	1 cut(s)	1574
BstACI	GRCGYC	1 cut(s)	240
BstAPI	GCANNNNNTGC	1 cut(s)	760
BstBAI	YACGTR	3 cut(s)	436, 1466, 1613
BstBI	TTCGAA	1 cut(s)	1134
BstC8I	GCNNGC	4 cut(s)	151, 314, 865, 1629
BstDEI	CTNAG	6 cut(s)	12, 65, 156, 954, 1155, 1290
BstDSI	CCRYGG	3 cut(s)	144, 393, 1528
BstF5I	GGATG	4 cut(s)	595, 1207, 1587, 1598
BstFNI	CGCG	7 cut(s)	146, 153, 188, 306, 312, 314, 453
BstH2I	RGCGCY	2 cut(s)	269, 513
BstHHI	GCGC	6 cut(s)	155, 268, 314, 316, 512, 1420
BstKTI	GATC	5 cut(s)	234, 324, 804, 1222, 1618
BstMAI	GTCTC	3 cut(s)	1001, 1151, 1172
BstMBI	GATC	5 cut(s)	231, 321, 801, 1219, 1615
BstMCI	CGRYCG	2 cut(s)	141, 310
BstNI	CCWGG	4 cut(s)	35, 122, 492, 710
BstPAI	GACNNNNGTC	2 cut(s)	257, 918
BstSCI	CCNGG	4 cut(s)	33, 120, 490, 708
BstSLI	GKGCMC	1 cut(s)	1522
BstUI	CGCG	7 cut(s)	146, 153, 188, 306, 312, 314, 453
BstV1I	GCAGC	4 cut(s)	121, 412, 580, 895
BstV2I	GAAGAC	1 cut(s)	1378
BstX2I	RGATCY	1 cut(s)	1219
BstYI	RGATCY	1 cut(s)	1219
BstZI	CGGCCG	2 cut(s)	138, 307
Bsu15I	ATCGAT	1 cut(s)	224
BsuI	GTATCC	3 cut(s)	655, 1137, 1436
BsuRI	GGCC	6 cut(s)	77, 140, 309, 902, 1263, 1330
BsuTUI	ATCGAT	1 cut(s)	224
BtgI	CCRYGG	3 cut(s)	144, 393, 1528
BtsCI	GGATG	4 cut(s)	595, 1207, 1587, 1598
BtsIMutI	CAGTG	3 cut(s)	389, 990, 1287
BveI	ACCTGC	3 cut(s)	511, 868, 1044
Cac8I	GCNNGC	4 cut(s)	151, 314, 865, 1629
CciNI	GCGGCCGC	2 cut(s)	138, 307
CfoI	GCGC	6 cut(s)	155, 268, 314, 316, 512, 1420
Cfr10I	RCCGGY	1 cut(s)	700
Cfr13I	GGNCC	5 cut(s)	175, 286, 938, 1231, 1261
Cfr42I	CCGCGG	1 cut(s)	147
ClaI	ATCGAT	1 cut(s)	224
CseI	GACGC	1 cut(s)	376
Csp6I	GTAC	7 cut(s)	433, 547, 683, 1115, 1147, 1366, 1433
CviAII	CATG	4 cut(s)	343, 1342, 1430, 1529
CviQI	GTAC	7 cut(s)	433, 547, 683, 1115, 1147, 1366, 1433
DdeI	CTNAG	6 cut(s)	12, 65, 156, 954, 1155, 1290
DpnI	GATC	5 cut(s)	233, 323, 803, 1221, 1617
DpnII	GATC	5 cut(s)	231, 321, 801, 1219, 1615
DraI	TTTAAA	1 cut(s)	838
DriI	GACNNNNNGTC	1 cut(s)	1164
EaeI	YGGCCR	3 cut(s)	138, 307, 900
EagI	CGGCCG	2 cut(s)	138, 307
Eam1104I	CTCTTC	1 cut(s)	1574
Eam1105I	GACNNNNNGTC	1 cut(s)	1164
EarI	CTCTTC	1 cut(s)	1574
EciI	GGCGGA	2 cut(s)	46, 151
EclXI	CGGCCG	2 cut(s)	138, 307
Eco130I	CCWWGG	1 cut(s)	1528
Eco147I	AGGCCT	1 cut(s)	1330
Eco24I	GRGCYC	1 cut(s)	30
Eco31I	GGTCTC	1 cut(s)	1001
Eco47I	GGWCC	4 cut(s)	175, 286, 938, 1231
Eco47III	AGCGCT	1 cut(s)	267
Eco52I	CGGCCG	2 cut(s)	138, 307
Eco57I	CTGAAG	6 cut(s)	733, 789, 1217, 1263, 1479, 1492
Eco72I	CACGTG	2 cut(s)	1466, 1613
Eco88I	CYCGRG	1 cut(s)	101
EcoO109I	RGGNCCY	2 cut(s)	175, 1231
EcoRII	CCWGG	4 cut(s)	33, 120, 490, 708
EcoT14I	CCWWGG	1 cut(s)	1528
EcoT38I	GRGCYC	1 cut(s)	30
ErhI	CCWWGG	1 cut(s)	1528
Esp3I	CGTCTC	1 cut(s)	1172
FaeI	CATG	4 cut(s)	346, 1345, 1433, 1532
FaqI	GGGAC	1 cut(s)	1244
FatI	CATG	4 cut(s)	342, 1341, 1429, 1528
FokI	GGATG	4 cut(s)	602, 1214, 1574, 1585
FriOI	GRGCYC	1 cut(s)	30
FspBI	CTAG	1 cut(s)	1628
FspI	TGCGCA	1 cut(s)	1419
GlaI	GCGC	6 cut(s)	154, 267, 313, 315, 511, 1419
GsuI	CTGGAG	2 cut(s)	654, 1571
HaeII	RGCGCY	2 cut(s)	269, 513
HaeIII	GGCC	6 cut(s)	77, 140, 309, 902, 1263, 1330
HapII	CCGG	4 cut(s)	170, 404, 701, 1624
HgaI	GACGC	1 cut(s)	376
HhaI	GCGC	6 cut(s)	155, 268, 314, 316, 512, 1420
Hin1I	GRCGYC	1 cut(s)	240
Hin1II	CATG	4 cut(s)	346, 1345, 1433, 1532
Hin6I	GCGC	6 cut(s)	153, 266, 312, 314, 510, 1418
HinP1I	GCGC	6 cut(s)	153, 266, 312, 314, 510, 1418
HincII	GTYRAC	1 cut(s)	244
HindII	GTYRAC	1 cut(s)	244
HinfI	GANTC	8 cut(s)	53, 158, 455, 723, 730, 959, 1159, 1502
HpaII	CCGG	4 cut(s)	170, 404, 701, 1624
HphI	GGTGA	5 cut(s)	783, 870, 923, 1298, 1409
Hpy166II	GTNNAC	3 cut(s)	244, 990, 1520
Hpy188III	TCNNGA	6 cut(s)	103, 334, 659, 727, 998, 1480
Hpy8I	GTNNAC	3 cut(s)	244, 990, 1520
Hpy99I	CGWCG	4 cut(s)	117, 242, 335, 392
HpyCH4III	ACNGT	4 cut(s)	379, 397, 551, 1370
HpyCH4IV	ACGT	7 cut(s)	240, 435, 1165, 1423, 1453, 1465, 1612
HpyCH4V	TGCA	3 cut(s)	400, 532, 1520
HpyF3I	CTNAG	6 cut(s)	12, 65, 156, 954, 1155, 1290
HpySE526I	ACGT	7 cut(s)	240, 435, 1165, 1423, 1453, 1465, 1612
Hsp92I	GRCGYC	1 cut(s)	240
Hsp92II	CATG	4 cut(s)	346, 1345, 1433, 1532
HspAI	GCGC	6 cut(s)	153, 266, 312, 314, 510, 1418
KspI	CCGCGG	1 cut(s)	147
Kzo9I	GATC	5 cut(s)	231, 321, 801, 1219, 1615
LguI	GCTCTTC	1 cut(s)	1574
Lsp1109I	GCAGC	4 cut(s)	121, 412, 580, 895
LweI	GCATC	5 cut(s)	580, 595, 985, 1192, 1554
MaeI	CTAG	1 cut(s)	1628
MaeII	ACGT	7 cut(s)	240, 435, 1165, 1423, 1453, 1465, 1612
MaeIII	GTNAC	4 cut(s)	876, 911, 1063, 1352
MalI	GATC	5 cut(s)	233, 323, 803, 1221, 1617
MbiI	CCGCTC	2 cut(s)	208, 1621
MboI	GATC	5 cut(s)	231, 321, 801, 1219, 1615
MboII	GAAGA	4 cut(s)	349, 739, 1378, 1561
MflI	RGATCY	1 cut(s)	1219
MhlI	GDGCHC	2 cut(s)	30, 1522
MlsI	TGGCCA	1 cut(s)	902
MluCI	AATT	6 cut(s)	501, 612, 1090, 1265, 1372, 1470
MluNI	TGGCCA	1 cut(s)	902
MlyI	GAGTC	6 cut(s)	62, 167, 464, 739, 953, 1153
MmeI	TCCRAC	2 cut(s)	241, 718
Mox20I	TGGCCA	1 cut(s)	902
MroXI	GAANNNNTTC	2 cut(s)	371, 1185
MscI	TGGCCA	1 cut(s)	902
MseI	TTAA	4 cut(s)	446, 504, 837, 1017
MslI	CAYNNNNRTG	1 cut(s)	981
Msp20I	TGGCCA	1 cut(s)	902
MspA1I	CMGCKG	1 cut(s)	146
MspI	CCGG	4 cut(s)	170, 404, 701, 1624
MspR9I	CCNGG	4 cut(s)	35, 122, 492, 710
MssI	GTTTAAAC	1 cut(s)	838
MvaI	CCWGG	4 cut(s)	35, 122, 492, 710
MvnI	CGCG	7 cut(s)	146, 153, 188, 306, 312, 314, 453
NcoI	CCATGG	1 cut(s)	1528
NdeII	GATC	5 cut(s)	231, 321, 801, 1219, 1615
NheI	GCTAGC	1 cut(s)	1627
NlaIII	CATG	4 cut(s)	346, 1345, 1433, 1532
NlaIV	GGNNCC	3 cut(s)	39, 177, 1232
NmeAIII	GCCGAG	1 cut(s)	190
NmuCI	GTSAC	3 cut(s)	876, 911, 1352
NotI	GCGGCCGC	2 cut(s)	138, 307
NsbI	TGCGCA	1 cut(s)	1419
NspV	TTCGAA	1 cut(s)	1134
PaeR7I	CTCGAG	1 cut(s)	101
PaqCI	CACCTGC	1 cut(s)	511
PauI	GCGCGC	1 cut(s)	312
PceI	AGGCCT	1 cut(s)	1330
PciSI	GCTCTTC	1 cut(s)	1574
PdmI	GAANNNNTTC	2 cut(s)	371, 1185
PfeI	GAWTC	2 cut(s)	723, 1502
PleI	GAGTC	6 cut(s)	61, 166, 463, 738, 953, 1153
PmaCI	CACGTG	2 cut(s)	1466, 1613
PmeI	GTTTAAAC	1 cut(s)	838
PmlI	CACGTG	2 cut(s)	1466, 1613
PpsI	GAGTC	6 cut(s)	61, 166, 463, 738, 953, 1153
Ppu21I	YACGTR	3 cut(s)	436, 1466, 1613
PpuMI	RGGWCCY	2 cut(s)	175, 1231
PshAI	GACNNNNGTC	2 cut(s)	257, 918
Psp5II	RGGWCCY	2 cut(s)	175, 1231
Psp6I	CCWGG	4 cut(s)	33, 120, 490, 708
PspCI	CACGTG	2 cut(s)	1466, 1613
PspGI	CCWGG	4 cut(s)	33, 120, 490, 708
PspN4I	GGNNCC	3 cut(s)	39, 177, 1232
PspPI	GGNCC	5 cut(s)	175, 286, 938, 1231, 1261
PspPPI	RGGWCCY	2 cut(s)	175, 1231
PsuI	RGATCY	1 cut(s)	1219
PteI	GCGCGC	1 cut(s)	312
RsaI	GTAC	7 cut(s)	434, 548, 684, 1116, 1148, 1367, 1434
RsaNI	GTAC	7 cut(s)	433, 547, 683, 1115, 1147, 1366, 1433
RseI	CAYNNNNRTG	1 cut(s)	981
SacII	CCGCGG	1 cut(s)	147
SapI	GCTCTTC	1 cut(s)	1574
SaqAI	TTAA	4 cut(s)	446, 504, 837, 1017
Sau3AI	GATC	5 cut(s)	231, 321, 801, 1219, 1615
Sau96I	GGNCC	5 cut(s)	175, 286, 938, 1231, 1261
ScaI	AGTACT	2 cut(s)	684, 1148
SchI	GAGTC	6 cut(s)	62, 167, 464, 739, 953, 1153
ScrFI	CCNGG	4 cut(s)	35, 122, 492, 710
SduI	GDGCHC	2 cut(s)	30, 1522
SfaNI	GCATC	5 cut(s)	580, 595, 985, 1192, 1554
Sfr274I	CTCGAG	1 cut(s)	101
Sfr303I	CCGCGG	1 cut(s)	147
SfuI	TTCGAA	1 cut(s)	1134
SgrBI	CCGCGG	1 cut(s)	147
SinI	GGWCC	4 cut(s)	175, 286, 938, 1231
SlaI	CTCGAG	1 cut(s)	101
SmiMI	CAYNNNNRTG	1 cut(s)	981
SmlI	CTYRAG	1 cut(s)	101
SmoI	CTYRAG	1 cut(s)	101
Sse9I	AATT	6 cut(s)	501, 612, 1090, 1265, 1372, 1470
SseBI	AGGCCT	1 cut(s)	1330
SspMI	CTAG	1 cut(s)	1628
StuI	AGGCCT	1 cut(s)	1330
StyD4I	CCNGG	4 cut(s)	33, 120, 490, 708
StyI	CCWWGG	1 cut(s)	1528
TaaI	ACNGT	4 cut(s)	379, 397, 551, 1370
TaiI	ACGT	7 cut(s)	243, 438, 1168, 1426, 1456, 1468, 1615
TaqI	TCGA	7 cut(s)	102, 224, 598, 999, 1134, 1212, 1393
TasI	AATT	6 cut(s)	501, 612, 1090, 1265, 1372, 1470
TatI	WGTACW	4 cut(s)	546, 682, 1146, 1365
TfiI	GAWTC	2 cut(s)	723, 1502
Tru1I	TTAA	4 cut(s)	446, 504, 837, 1017
Tru9I	TTAA	4 cut(s)	446, 504, 837, 1017
TscAI	CASTG	3 cut(s)	389, 997, 1294
TseFI	GTSAC	3 cut(s)	876, 911, 1352
TseI	GCWGC	4 cut(s)	134, 400, 593, 883
Tsp45I	GTSAC	3 cut(s)	876, 911, 1352
TspDTI	ATGAA	2 cut(s)	1365, 1378
TspGWI	ACGGA	6 cut(s)	101, 269, 287, 820, 1132, 1305
TspRI	CASTG	3 cut(s)	389, 997, 1294
VneI	GTGCAC	1 cut(s)	1518
VpaK11BI	GGWCC	4 cut(s)	175, 286, 938, 1231
XcmI	CCANNNNNNNNNTGG	2 cut(s)	734, 1535
XhoI	CTCGAG	1 cut(s)	101
XmnI	GAANNNNTTC	2 cut(s)	371, 1185
XspI	CTAG	1 cut(s)	1628
ZraI	GACGTC	1 cut(s)	241
ZrmI	AGTACT	2 cut(s)	684, 1148

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.