Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0001714

Physical Location & Seq

Genomic Coordinates Forward (+)

66763048 .. 66766182

Gene Structure 3135 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG515600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1626 bp

ATGTCAGAACCTCAGCCACCCTCCGAGCCAGAGAACGAGGAGTCCGCCTCACTCAGCAAAAAGGCCGCCAAGAAAGAAGCCGCCAAGCTCGAGAAGCTCCGTCGCCGCCAGGAGCAAGAAGCGGCGGCGGCGGCTTCTCGCGCTGAGTCCGCCGAGCCGGAAGACCCACTCGCCGCCAACTACGGCGACTTGCCGCTCATTCAGCTCCAATCGATGAGGATCGCCGACGTCAACGACTGGACGGAGGTCGGGGCGCTGACGGAGGCGTTGAAGGACCAGAAGGTTCTGATACGCGGCCGCGCGCAGACGATCCGAGCCGTCGGGAAGAACATGGCGTTTATTGTCGTCAGAGAGAAGGGCTTCACGGTTCAGTGCGTCGCCACGGTGCAGCCGGAGATTGTCAGCCGTCAGATGGTCAAGTACGTGGCGGCGTTGAACCGCGAGTCCATTATTGACATTGAAGGGATTGTGTCTGTTCCTGGCGTGGCAATTAAAGGCGCTTCACAGCAGGTGGAGGTGCAAGTCAGAAAGTTGTACTGTATAAGTAAGGCGGCTGTTTTGCCTATCAACATTGAGGATGCTGCTCGAAGTGATGCCGAAATTGAAAAAGCTAATCAGGCTGGAGAACAACTGGTTCGTGTAAATCAGGATACTCGCTTGAACTTCCGAGTACTTGACTTGCGAACACCGGCTAACCAGGGGATTTTCCGAATCCAGAGTCAAGTTGGAACTATCTTCAGGCAGTTCTTGCTGTCTGAAGGCTTTTTTGAAATCCACTCACCAAAACTGATCGCTGGTTCAAGTGAAGGTGGGGCTTCGGTGTTTAAACTGGACTACAAGGGGCAACCTGCCTGCCTTGCCCAGTCACCGCAGCTCCACAAGCAGATGGCCATCTGTGGTGACTTTGGTCGTATTTTTGAGATTGGTCCTGTTTTCAGAGCTGAGGACTCCTATACACATAGGCATCTATGTGAGTTCACTGGTCTCGATATTGAGATGGAGATTAAGTGGAGCTATTTTGAGGTTATGGATATTGTTAGCAGGTTGTTTGTTACTATGTTTGACTATTTGAACAAAAATTGTGAGAAGGAACTTGAAGCTGTACGGAGGCAGTATCCCTTCGAACCACTAAAGTACTTGCCTGAGACTCTACGTCTCACTTTTGCGGAAGGTGTTCAAATGCTGAAGGATGCTGGTGTCGAAGTAGATCCTATGGGGGACCTAAATACTGAAGCAGAGAGGAAGTTGGGCCAATTAGTTTTGGAAAAATATGGCACTGAGTTCTACATACTTCACCGCTATCCGTTGGCTGTGAGGCCTTTCTATACCATGCCTTGCTGTGACAATAAGGAGTACAGTAATTCATTTGATGTCTTCATTCGCGGTGAGGAAATTATATCAGGTGCGCAACGTGTCCATGTACCAGAGTTCTTGGCTGAACGTGCTGAAGCACGTGGAATTGAAGTCAAGACAATATCAACATATATTGATTCCTTCAGATATGGTGCACCTCCCCATGGTGGATTTGGAGTAGGGCTGGAGCGTGTTGTGATGCTCTTCTGTGGTTTGAATAACATCCGCAAAACATCCTTATTCCCCCGTGATCCGCTTCGGCTAGCTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004815 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006422 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0012505 GO:0016070 GO:0016874 GO:0016875 GO:0017101 GO:0019538 GO:0019752 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045202 GO:0046483 GO:0046686 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0090304 GO:0098542 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2 tRNA_anti-codon

Protein Analysis

541

Amino Acids

60.71

Weight (kDa)

5.77

Isoelectric Point (pI)

48.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2	PF00152	215 - 535	4.1e-77	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001714)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26870 AT4G31180 AT4G31180
fragaria_vesca	FvH4_2g15870 FvH4_2g15910 FvH4_2g30550
malus_domestica	MD08G1134400.v1.1 MD12G1153800.v1.1 MD14G1143300.v1.1 MD15G1113400.v1.1
prunus_persica	Prupe.1G463800_v2.0.a1 Prupe.4G141600_v2.0.a1 Prupe.8G055000_v2.0.a1
pyrus_communis	pycom08g11340 pycom15g10170 pycom16g11710
rosa_chinensis	RchiOBHm_Chr5g0032141 RchiOBHm_Chr5g0032161 RchiOBHm_Chr6g0278871 RchiOBHm_Chr6g0312531
rosa_laevigata	RLG00000010321 RLG00000033368
rosa_multiflora	Rmu_co8427103.1_g000001 Rmu_sc0003031.1_g000023 Rmu_sc0005375.1_g000002 Rmu_sc0005375.1_g000011 Rmu_sc0008363.1_g000011 Rmu_sc0043080.1_g000001
rosa_roxburghii	Rroxscaffold_1G00048240 Rroxscaffold_1G00048270 Rroxscaffold_7G00156770
rosa_rugosa	Rorug06G0119800 Rorug06G0120000 Rorug06G0123400 Rorug06G0396100 Rorug06G0396200
rosa_samantha	Rh5DG228900 Rh6AG228300 Rh6AG513500 Rh6BG233900 Rh6BG234000 Rh6BG524200 Rh6BG526500 Rh6CG236300 Rh6CG531400 Rh6DG515600
rosa_wichuraiana	Rw6G020030 Rw6G044650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	499
AatII	GACGTC	1 cut(s)	231
Acc16I	TGCGCA	1 cut(s)	1407
Acc36I	ACCTGC	3 cut(s)	499, 856, 1032
AccBSI	CCGCTC	1 cut(s)	196
AccII	CGCG	6 cut(s)	141, 294, 300, 302, 441, 1383
AclWI	GGATC	4 cut(s)	227, 304, 1202, 1598
AcoI	YGGCCR	2 cut(s)	295, 888
AcuI	CTGAAG	6 cut(s)	721, 777, 1205, 1251, 1467, 1480
AcvI	CACGTG	1 cut(s)	1454
AcyI	GRCGYC	1 cut(s)	228
AfaI	GTAC	7 cut(s)	422, 536, 672, 1104, 1136, 1355, 1422
AfiI	CCNNNNNNNGG	3 cut(s)	412, 1517, 1520
AflIII	ACRYGT	1 cut(s)	1411
AhdI	GACNNNNNGTC	1 cut(s)	1152
AjnI	CCWGG	3 cut(s)	108, 478, 696
AloI	GAACNNNNNNTCC	4 cut(s)	26, 58, 428, 460
AluBI	AGCT	9 cut(s)	88, 97, 205, 611, 874, 941, 1014, 1100, 1619
AluI	AGCT	9 cut(s)	88, 97, 205, 611, 874, 941, 1014, 1100, 1619
Alw21I	GWGCWC	1 cut(s)	1510
Alw26I	GTCTC	3 cut(s)	989, 1139, 1160
Alw44I	GTGCAC	1 cut(s)	1506
AlwI	GGATC	4 cut(s)	227, 304, 1202, 1598
Ama87I	CYCGRG	1 cut(s)	89
AoxI	GGCC	5 cut(s)	63, 295, 888, 1249, 1316
ApaLI	GTGCAC	1 cut(s)	1506
ApeKI	GCWGC	3 cut(s)	388, 581, 871
ArsI	GACNNNNNNTTYG	2 cut(s)	703, 735
Asp700I	GAANNNNTTC	2 cut(s)	359, 1173
AspLEI	GCGC	6 cut(s)	143, 256, 302, 304, 500, 1408
AspS9I	GGNCC	4 cut(s)	274, 926, 1219, 1249
AsuHPI	GGTGA	5 cut(s)	771, 858, 911, 1286, 1397
AsuII	TTCGAA	1 cut(s)	1122
AsuNHI	GCTAGC	1 cut(s)	1615
AvaI	CYCGRG	1 cut(s)	89
AvaII	GGWCC	3 cut(s)	274, 926, 1219
BaeGI	GKGCMC	1 cut(s)	1510
BalI	TGGCCA	1 cut(s)	890
BbrPI	CACGTG	1 cut(s)	1454
BbsI	GAAGAC	2 cut(s)	168, 1366
Bbv12I	GWGCWC	1 cut(s)	1510
BbvCI	CCTCAGC	2 cut(s)	12, 942
BbvI	GCAGC	3 cut(s)	400, 568, 883
BccI	CCATC	4 cut(s)	406, 880, 899, 991
BceAI	ACGGC	3 cut(s)	199, 302, 390
BciT130I	CCWGG	3 cut(s)	110, 480, 698
BciVI	GTATCC	2 cut(s)	643, 1125
BcoDI	GTCTC	3 cut(s)	989, 1139, 1160
BfaI	CTAG	1 cut(s)	1616
BfoI	RGCGCY	2 cut(s)	257, 501
BfuAI	ACCTGC	3 cut(s)	499, 856, 1032
BfuI	GTATCC	2 cut(s)	643, 1125
BmcAI	AGTACT	2 cut(s)	672, 1136
Bme1390I	CCNGG	3 cut(s)	110, 480, 698
Bme18I	GGWCC	3 cut(s)	274, 926, 1219
BmeRI	GACNNNNNGTC	1 cut(s)	1152
BmeT110I	CYCGRG	1 cut(s)	89
BmgT120I	GGNCC	4 cut(s)	274, 926, 1219, 1249
BmiI	GGNNCC	1 cut(s)	1220
BmrFI	CCNGG	3 cut(s)	110, 480, 698
BmrI	ACTGGG	1 cut(s)	856
BmsI	GCATC	5 cut(s)	568, 583, 973, 1180, 1542
BmtI	GCTAGC	1 cut(s)	1619
BmuI	ACTGGG	1 cut(s)	856
BoxI	GACNNNNGTC	2 cut(s)	245, 906
BpiI	GAAGAC	2 cut(s)	168, 1366
BplI	GAGNNNNNCTC	2 cut(s)	32, 64
BpmI	CTGGAG	2 cut(s)	642, 1559
Bpu10I	CCTNAGC	2 cut(s)	12, 942
Bpu14I	TTCGAA	1 cut(s)	1122
Bsa29I	ATCGAT	1 cut(s)	212
BsaAI	YACGTR	2 cut(s)	424, 1454
BsaBI	GATNNNNATC	2 cut(s)	218, 890
BsaHI	GRCGYC	1 cut(s)	228
BsaI	GGTCTC	1 cut(s)	989
BsaJI	CCNNGG	3 cut(s)	381, 697, 1516
BsaXI	ACNNNNNCTCC	4 cut(s)	858, 888, 992, 1022
Bsc4I	CCNNNNNNNGG	3 cut(s)	412, 1517, 1520
Bse118I	RCCGGY	1 cut(s)	688
Bse1I	ACTGG	5 cut(s)	242, 636, 834, 862, 985
Bse8I	GATNNNNATC	2 cut(s)	218, 890
BseBI	CCWGG	3 cut(s)	110, 480, 698
BseCI	ATCGAT	1 cut(s)	212
BseDI	CCNNGG	3 cut(s)	381, 697, 1516
BseGI	GGATG	4 cut(s)	583, 1195, 1575, 1586
BseJI	GATNNNNATC	2 cut(s)	218, 890
BseLI	CCNNNNNNNGG	3 cut(s)	412, 1517, 1520
BseMII	CTCAG	6 cut(s)	26, 67, 135, 933, 1134, 1269
BseNI	ACTGG	5 cut(s)	242, 636, 834, 862, 985
BsePI	GCGCGC	1 cut(s)	300
BseRI	GAGGAG	1 cut(s)	53
BseSI	GKGCMC	1 cut(s)	1510
BseX3I	CGGCCG	1 cut(s)	295
BseXI	GCAGC	3 cut(s)	400, 568, 883
BsgI	GTGCAG	1 cut(s)	407
Bsh1236I	CGCG	6 cut(s)	141, 294, 300, 302, 441, 1383
Bsh1285I	CGRYCG	1 cut(s)	298
BshFI	GGCC	5 cut(s)	65, 297, 890, 1251, 1318
BshVI	ATCGAT	1 cut(s)	212
BsiEI	CGRYCG	1 cut(s)	298
BsiHKAI	GWGCWC	1 cut(s)	1510
BsiHKCI	CYCGRG	1 cut(s)	89
BsiSI	CCGG	3 cut(s)	158, 392, 689
BslFI	GGGAC	1 cut(s)	1232
BslI	CCNNNNNNNGG	3 cut(s)	412, 1517, 1520
BsmAI	GTCTC	3 cut(s)	989, 1139, 1160
BsmBI	CGTCTC	1 cut(s)	1160
BsmFI	GGGAC	1 cut(s)	1232
BsnI	GGCC	5 cut(s)	65, 297, 890, 1251, 1318
Bso31I	GGTCTC	1 cut(s)	989
BsoBI	CYCGRG	1 cut(s)	89
Bsp119I	TTCGAA	1 cut(s)	1122
Bsp1286I	GDGCHC	1 cut(s)	1510
Bsp143I	GATC	5 cut(s)	219, 309, 789, 1207, 1603
Bsp19I	CCATGG	1 cut(s)	1516
BspANI	GGCC	5 cut(s)	65, 297, 890, 1251, 1318
BspCNI	CTCAG	6 cut(s)	25, 66, 136, 934, 1135, 1270
BspDI	ATCGAT	1 cut(s)	212
BspFNI	CGCG	6 cut(s)	141, 294, 300, 302, 441, 1383
BspLI	GGNNCC	1 cut(s)	1220
BspMI	ACCTGC	3 cut(s)	499, 856, 1032
BspOI	GCTAGC	1 cut(s)	1619
BspPI	GGATC	4 cut(s)	227, 304, 1202, 1598
BspQI	GCTCTTC	1 cut(s)	1562
BspT104I	TTCGAA	1 cut(s)	1122
BspTNI	GGTCTC	1 cut(s)	989
BsrBI	CCGCTC	1 cut(s)	196
BsrFI	RCCGGY	1 cut(s)	688
BsrI	ACTGG	5 cut(s)	242, 636, 834, 862, 985
BssAI	RCCGGY	1 cut(s)	688
BssECI	CCNNGG	3 cut(s)	381, 697, 1516
BssHII	GCGCGC	1 cut(s)	300
BssMI	GATC	5 cut(s)	219, 309, 789, 1207, 1603
BssNI	GRCGYC	1 cut(s)	228
BssT1I	CCWWGG	1 cut(s)	1516
Bst2UI	CCWGG	3 cut(s)	110, 480, 698
Bst4CI	ACNGT	4 cut(s)	367, 385, 539, 1358
Bst6I	CTCTTC	1 cut(s)	1562
BstACI	GRCGYC	1 cut(s)	228
BstAPI	GCANNNNNTGC	1 cut(s)	748
BstBAI	YACGTR	2 cut(s)	424, 1454
BstBI	TTCGAA	1 cut(s)	1122
BstC8I	GCNNGC	3 cut(s)	302, 853, 1617
BstDEI	CTNAG	6 cut(s)	12, 53, 144, 942, 1143, 1278
BstDSI	CCRYGG	2 cut(s)	381, 1516
BstF5I	GGATG	4 cut(s)	583, 1195, 1575, 1586
BstFNI	CGCG	6 cut(s)	141, 294, 300, 302, 441, 1383
BstH2I	RGCGCY	2 cut(s)	257, 501
BstHHI	GCGC	6 cut(s)	143, 256, 302, 304, 500, 1408
BstKTI	GATC	5 cut(s)	222, 312, 792, 1210, 1606
BstMAI	GTCTC	3 cut(s)	989, 1139, 1160
BstMBI	GATC	5 cut(s)	219, 309, 789, 1207, 1603
BstMCI	CGRYCG	1 cut(s)	298
BstNI	CCWGG	3 cut(s)	110, 480, 698
BstPAI	GACNNNNGTC	2 cut(s)	245, 906
BstSCI	CCNGG	3 cut(s)	108, 478, 696
BstSLI	GKGCMC	1 cut(s)	1510
BstUI	CGCG	6 cut(s)	141, 294, 300, 302, 441, 1383
BstV1I	GCAGC	3 cut(s)	400, 568, 883
BstV2I	GAAGAC	2 cut(s)	168, 1366
BstX2I	RGATCY	1 cut(s)	1207
BstYI	RGATCY	1 cut(s)	1207
BstZI	CGGCCG	1 cut(s)	295
Bsu15I	ATCGAT	1 cut(s)	212
BsuI	GTATCC	2 cut(s)	643, 1125
BsuRI	GGCC	5 cut(s)	65, 297, 890, 1251, 1318
BsuTUI	ATCGAT	1 cut(s)	212
BtgI	CCRYGG	2 cut(s)	381, 1516
BtsCI	GGATG	4 cut(s)	583, 1195, 1575, 1586
BtsIMutI	CAGTG	3 cut(s)	377, 978, 1275
BveI	ACCTGC	3 cut(s)	499, 856, 1032
Cac8I	GCNNGC	3 cut(s)	302, 853, 1617
CciNI	GCGGCCGC	1 cut(s)	295
CfoI	GCGC	6 cut(s)	143, 256, 302, 304, 500, 1408
Cfr10I	RCCGGY	1 cut(s)	688
Cfr13I	GGNCC	4 cut(s)	274, 926, 1219, 1249
ClaI	ATCGAT	1 cut(s)	212
CseI	GACGC	1 cut(s)	364
Csp6I	GTAC	7 cut(s)	421, 535, 671, 1103, 1135, 1354, 1421
CviAII	CATG	4 cut(s)	331, 1330, 1418, 1517
CviQI	GTAC	7 cut(s)	421, 535, 671, 1103, 1135, 1354, 1421
DdeI	CTNAG	6 cut(s)	12, 53, 144, 942, 1143, 1278
DpnI	GATC	5 cut(s)	221, 311, 791, 1209, 1605
DpnII	GATC	5 cut(s)	219, 309, 789, 1207, 1603
DraI	TTTAAA	1 cut(s)	826
DriI	GACNNNNNGTC	1 cut(s)	1152
EaeI	YGGCCR	2 cut(s)	295, 888
EagI	CGGCCG	1 cut(s)	295
Eam1104I	CTCTTC	1 cut(s)	1562
Eam1105I	GACNNNNNGTC	1 cut(s)	1152
EarI	CTCTTC	1 cut(s)	1562
EciI	GGCGGA	2 cut(s)	34, 139
EclXI	CGGCCG	1 cut(s)	295
Eco130I	CCWWGG	1 cut(s)	1516
Eco147I	AGGCCT	1 cut(s)	1318
Eco31I	GGTCTC	1 cut(s)	989
Eco47I	GGWCC	3 cut(s)	274, 926, 1219
Eco52I	CGGCCG	1 cut(s)	295
Eco57I	CTGAAG	6 cut(s)	721, 777, 1205, 1251, 1467, 1480
Eco72I	CACGTG	1 cut(s)	1454
Eco88I	CYCGRG	1 cut(s)	89
EcoO109I	RGGNCCY	1 cut(s)	1219
EcoRII	CCWGG	3 cut(s)	108, 478, 696
EcoT14I	CCWWGG	1 cut(s)	1516
ErhI	CCWWGG	1 cut(s)	1516
Esp3I	CGTCTC	1 cut(s)	1160
FaeI	CATG	4 cut(s)	334, 1333, 1421, 1520
FaqI	GGGAC	1 cut(s)	1232
FatI	CATG	4 cut(s)	330, 1329, 1417, 1516
FokI	GGATG	4 cut(s)	590, 1202, 1562, 1573
FspBI	CTAG	1 cut(s)	1616
FspI	TGCGCA	1 cut(s)	1407
GlaI	GCGC	6 cut(s)	142, 255, 301, 303, 499, 1407
GsuI	CTGGAG	2 cut(s)	642, 1559
HaeII	RGCGCY	2 cut(s)	257, 501
HaeIII	GGCC	5 cut(s)	65, 297, 890, 1251, 1318
HapII	CCGG	3 cut(s)	158, 392, 689
HgaI	GACGC	1 cut(s)	364
HhaI	GCGC	6 cut(s)	143, 256, 302, 304, 500, 1408
Hin1I	GRCGYC	1 cut(s)	228
Hin1II	CATG	4 cut(s)	334, 1333, 1421, 1520
Hin6I	GCGC	6 cut(s)	141, 254, 300, 302, 498, 1406
HinP1I	GCGC	6 cut(s)	141, 254, 300, 302, 498, 1406
HincII	GTYRAC	1 cut(s)	232
HindII	GTYRAC	1 cut(s)	232
HinfI	GANTC	8 cut(s)	41, 146, 443, 711, 718, 947, 1147, 1490
HpaII	CCGG	3 cut(s)	158, 392, 689
HphI	GGTGA	5 cut(s)	771, 858, 911, 1286, 1397
Hpy166II	GTNNAC	3 cut(s)	232, 978, 1508
Hpy188III	TCNNGA	6 cut(s)	91, 322, 647, 715, 986, 1468
Hpy8I	GTNNAC	3 cut(s)	232, 978, 1508
Hpy99I	CGWCG	4 cut(s)	105, 230, 323, 380
HpyCH4III	ACNGT	4 cut(s)	367, 385, 539, 1358
HpyCH4IV	ACGT	6 cut(s)	228, 423, 1153, 1411, 1441, 1453
HpyCH4V	TGCA	3 cut(s)	388, 520, 1508
HpyF3I	CTNAG	6 cut(s)	12, 53, 144, 942, 1143, 1278
HpySE526I	ACGT	6 cut(s)	228, 423, 1153, 1411, 1441, 1453
Hsp92I	GRCGYC	1 cut(s)	228
Hsp92II	CATG	4 cut(s)	334, 1333, 1421, 1520
HspAI	GCGC	6 cut(s)	141, 254, 300, 302, 498, 1406
Kzo9I	GATC	5 cut(s)	219, 309, 789, 1207, 1603
LguI	GCTCTTC	1 cut(s)	1562
LmnI	GCTCC	7 cut(s)	102, 112, 210, 879, 1011, 1540, 1624
Lsp1109I	GCAGC	3 cut(s)	400, 568, 883
LweI	GCATC	5 cut(s)	568, 583, 973, 1180, 1542
MaeI	CTAG	1 cut(s)	1616
MaeII	ACGT	6 cut(s)	228, 423, 1153, 1411, 1441, 1453
MaeIII	GTNAC	4 cut(s)	864, 899, 1051, 1340
MalI	GATC	5 cut(s)	221, 311, 791, 1209, 1605
MbiI	CCGCTC	1 cut(s)	196
MboI	GATC	5 cut(s)	219, 309, 789, 1207, 1603
MboII	GAAGA	5 cut(s)	173, 337, 727, 1366, 1549
MflI	RGATCY	1 cut(s)	1207
MhlI	GDGCHC	1 cut(s)	1510
MlsI	TGGCCA	1 cut(s)	890
MluCI	AATT	7 cut(s)	489, 600, 1078, 1253, 1360, 1392, 1458
MluNI	TGGCCA	1 cut(s)	890
MlyI	GAGTC	6 cut(s)	50, 155, 452, 727, 941, 1141
MmeI	TCCRAC	1 cut(s)	706
Mox20I	TGGCCA	1 cut(s)	890
MroXI	GAANNNNTTC	2 cut(s)	359, 1173
MscI	TGGCCA	1 cut(s)	890
MseI	TTAA	3 cut(s)	492, 825, 1005
MslI	CAYNNNNRTG	1 cut(s)	969
Msp20I	TGGCCA	1 cut(s)	890
MspI	CCGG	3 cut(s)	158, 392, 689
MspR9I	CCNGG	3 cut(s)	110, 480, 698
MssI	GTTTAAAC	1 cut(s)	826
MvaI	CCWGG	3 cut(s)	110, 480, 698
MvnI	CGCG	6 cut(s)	141, 294, 300, 302, 441, 1383
NcoI	CCATGG	1 cut(s)	1516
NdeII	GATC	5 cut(s)	219, 309, 789, 1207, 1603
NheI	GCTAGC	1 cut(s)	1615
NlaIII	CATG	4 cut(s)	334, 1333, 1421, 1520
NlaIV	GGNNCC	1 cut(s)	1220
NmeAIII	GCCGAG	1 cut(s)	178
NmuCI	GTSAC	3 cut(s)	864, 899, 1340
NotI	GCGGCCGC	1 cut(s)	295
NsbI	TGCGCA	1 cut(s)	1407
NspV	TTCGAA	1 cut(s)	1122
PaeR7I	CTCGAG	1 cut(s)	89
PaqCI	CACCTGC	1 cut(s)	499
PauI	GCGCGC	1 cut(s)	300
PceI	AGGCCT	1 cut(s)	1318
PciSI	GCTCTTC	1 cut(s)	1562
PdmI	GAANNNNTTC	2 cut(s)	359, 1173
PfeI	GAWTC	2 cut(s)	711, 1490
PleI	GAGTC	6 cut(s)	49, 154, 451, 726, 941, 1141
PmaCI	CACGTG	1 cut(s)	1454
PmeI	GTTTAAAC	1 cut(s)	826
PmlI	CACGTG	1 cut(s)	1454
PpsI	GAGTC	6 cut(s)	49, 154, 451, 726, 941, 1141
Ppu21I	YACGTR	2 cut(s)	424, 1454
PpuMI	RGGWCCY	1 cut(s)	1219
PshAI	GACNNNNGTC	2 cut(s)	245, 906
Psp5II	RGGWCCY	1 cut(s)	1219
Psp6I	CCWGG	3 cut(s)	108, 478, 696
PspCI	CACGTG	1 cut(s)	1454
PspGI	CCWGG	3 cut(s)	108, 478, 696
PspN4I	GGNNCC	1 cut(s)	1220
PspPI	GGNCC	4 cut(s)	274, 926, 1219, 1249
PspPPI	RGGWCCY	1 cut(s)	1219
PsuI	RGATCY	1 cut(s)	1207
PteI	GCGCGC	1 cut(s)	300
RsaI	GTAC	7 cut(s)	422, 536, 672, 1104, 1136, 1355, 1422
RsaNI	GTAC	7 cut(s)	421, 535, 671, 1103, 1135, 1354, 1421
RseI	CAYNNNNRTG	1 cut(s)	969
SapI	GCTCTTC	1 cut(s)	1562
SaqAI	TTAA	3 cut(s)	492, 825, 1005
Sau3AI	GATC	5 cut(s)	219, 309, 789, 1207, 1603
Sau96I	GGNCC	4 cut(s)	274, 926, 1219, 1249
ScaI	AGTACT	2 cut(s)	672, 1136
SchI	GAGTC	6 cut(s)	50, 155, 452, 727, 941, 1141
ScrFI	CCNGG	3 cut(s)	110, 480, 698
SduI	GDGCHC	1 cut(s)	1510
SfaNI	GCATC	5 cut(s)	568, 583, 973, 1180, 1542
Sfr274I	CTCGAG	1 cut(s)	89
SfuI	TTCGAA	1 cut(s)	1122
SinI	GGWCC	3 cut(s)	274, 926, 1219
SlaI	CTCGAG	1 cut(s)	89
SmiMI	CAYNNNNRTG	1 cut(s)	969
SmlI	CTYRAG	1 cut(s)	89
SmoI	CTYRAG	1 cut(s)	89
Sse9I	AATT	7 cut(s)	489, 600, 1078, 1253, 1360, 1392, 1458
SseBI	AGGCCT	1 cut(s)	1318
SspMI	CTAG	1 cut(s)	1616
StuI	AGGCCT	1 cut(s)	1318
StyD4I	CCNGG	3 cut(s)	108, 478, 696
StyI	CCWWGG	1 cut(s)	1516
TaaI	ACNGT	4 cut(s)	367, 385, 539, 1358
TaiI	ACGT	6 cut(s)	231, 426, 1156, 1414, 1444, 1456
TaqI	TCGA	6 cut(s)	90, 212, 586, 987, 1122, 1200
TasI	AATT	7 cut(s)	489, 600, 1078, 1253, 1360, 1392, 1458
TatI	WGTACW	4 cut(s)	534, 670, 1134, 1353
TfiI	GAWTC	2 cut(s)	711, 1490
Tru1I	TTAA	3 cut(s)	492, 825, 1005
Tru9I	TTAA	3 cut(s)	492, 825, 1005
TscAI	CASTG	3 cut(s)	377, 985, 1282
TseFI	GTSAC	3 cut(s)	864, 899, 1340
TseI	GCWGC	3 cut(s)	388, 581, 871
Tsp45I	GTSAC	3 cut(s)	864, 899, 1340
TspDTI	ATGAA	2 cut(s)	1353, 1366
TspGWI	ACGGA	5 cut(s)	89, 257, 275, 1120, 1293
TspRI	CASTG	3 cut(s)	377, 985, 1282
VneI	GTGCAC	1 cut(s)	1506
VpaK11BI	GGWCC	3 cut(s)	274, 926, 1219
XcmI	CCANNNNNNNNNTGG	2 cut(s)	722, 1523
XhoI	CTCGAG	1 cut(s)	89
XmnI	GAANNNNTTC	2 cut(s)	359, 1173
XspI	CTAG	1 cut(s)	1616
ZraI	GACGTC	1 cut(s)	229
ZrmI	AGTACT	2 cut(s)	672, 1136

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.