FvH4_3g44966

Protein of unknown function (DUF3752)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001274
Physical Location & Seq
Forward (+)
37458593 .. 37459871
1279 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44966.t1

Sequence Viewer

Length: 1212 bp
ATGAAACCGAAAGTCAACGATAAGAAGAAGAAGAAGAGAGATCATGAGATTAATGATGATTCTGATTCTGTTTCTGGTGGTGATCCGCAAGCGGTTGTGCGAGACTTGTTGAAGGAGTTTCCGAATATTGGGTGCGATTTGAAGCAGCTTTTGCAATTGATCGATAGCGGCAAAGCGGTCGATATAACCGGAATAGCTCAGAAATCTCTGATCAAGCCTCTCAGAGGTCTCTTCCTTTGCTTGCATCTCAAGGAACTAAATCACGGAGCTTTCGTGTTGCCTCCACATGTTCCTCCAACTCTGGAGGTTATTGGGTCCATGATCACGCCTTGTTTAGATGATGATGATGCTCGACCAACTGAAAGAAGAAGGCCAATTGGGCCGGAGATGCCGACGCCGGAGCTACTTGCAGCTGCTGCCAAATTGAGAGAGGCAGAGGCGGAATTGAGGGAAGCTCAGTTGGAAGAAAAAGGTAATCGGGTTTTTATAGGACCTGCACCGCGTGTTGTAGTGGCTGAAGCTGAATCAGCTAACCAGGGTGATCGTTTCGAAGAGGTGACAAGGATCATGGAAGCAGAGGATGATAGCTTGTATGACATATTGGGAGTAGACCACTATGTGTCTGCTGATGACATCAAGAAAAGGTACTGGAAGTTGTCGCTTTTGGTGCACCCTGATAAATGCTCACACCCTCAAGCCAGCCAAGCATTTGTGAAGCTGAACAAGGCGTTTAAGGAGTTACAGGATCCGGAGAAGCGGAGAGCGCTGGATGAGAAGATTGAGCTCAAAGAGAAGCAGGAGAGATTCAAAAAGAGAAACATGGCAGGTGATGTGAGTGTTGTTAAATCGGCACCAAGAAGAGATGAGTGGATGACAGAGCTCCCACGGGAGAGGAAACATGGTATGAGTACAATGCAATCGACAATGTTTAGCAGCAATGCTAAAGAAGGGCGTGGAGATACTGGTTGTTGGACTGATGCTCCATCTGAAAGAGCTCGGAAGGCTAAGATGGATTACTTAAAAGCCTATAATGAGTTTTCATCGCTTGCTTCGAATGAGGAGGAGAGGAAGAGGAGTTTAGATGCAGAGTTGATGGACAGATGCAATAAAGAGAAACGATCGAAGTCATTGTTACAAAAGCACAAAGAGGAGATTAGAGCGAGCCGTTTGAAGAGGAAGCACTCCAAGCAACAGGTGGTAGACAAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001664 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0050780
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3752 DnaJ

Protein Analysis

404

Amino Acids

45.87

Weight (kDa)

8.78

Isoelectric Point (pI)

51.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 197 - 257 4.6e-19 DnaJ domain
DUF3752 PF12572 303 - 389 3.9e-12 Protein of unknown function (DUF3752)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 815
Acc36I ACCTGC 2 cut(s) 502, 815
AccB1I GGYRCC 1 cut(s) 850
AccI GTMKAC 2 cut(s) 609, 1200
AccII CGCG 1 cut(s) 502
AccIII TCCGGA 1 cut(s) 748
AciI CCGC 7 cut(s) 86, 92, 168, 176, 440, 500, 757
AclWI GGATC 4 cut(s) 77, 572, 740, 753
AcuI CTGAAG 1 cut(s) 537
AcyI GRCGYC 1 cut(s) 395
AdeI CACNNNGTG 2 cut(s) 503, 619
AfaI GTAC 2 cut(s) 647, 910
AfeI AGCGCT 1 cut(s) 765
AfiI CCNNNNNNNGG 2 cut(s) 128, 224
AflIII ACRYGT 1 cut(s) 286
AgsI TTSAA 4 cut(s) 112, 142, 808, 1171
AjnI CCWGG 1 cut(s) 534
Alw21I GWGCWC 4 cut(s) 672, 786, 882, 997
Alw26I GTCTC 2 cut(s) 96, 233
Alw44I GTGCAC 1 cut(s) 668
AlwI GGATC 4 cut(s) 77, 572, 740, 753
AlwNI CAGNNNCTG 1 cut(s) 416
Aor13HI TCCGGA 1 cut(s) 748
Aor51HI AGCGCT 1 cut(s) 765
AoxI GGCC 2 cut(s) 371, 380
ApaLI GTGCAC 1 cut(s) 668
ApeKI GCWGC 5 cut(s) 145, 410, 413, 416, 933
AseI ATTAAT 1 cut(s) 51
AspLEI GCGC 1 cut(s) 766
AspS9I GGNCC 3 cut(s) 315, 380, 491
AsuHPI GGTGA 4 cut(s) 92, 551, 568, 839
AsuII TTCGAA 2 cut(s) 549, 1052
AvaII GGWCC 2 cut(s) 315, 491
BaeGI GKGCMC 1 cut(s) 672
BamHI GGATCC 1 cut(s) 745
BanI GGYRCC 1 cut(s) 850
BanII GRGCYC 3 cut(s) 786, 882, 997
Bbv12I GWGCWC 4 cut(s) 672, 786, 882, 997
BbvI GCAGC 5 cut(s) 157, 400, 403, 422, 945
BccI CCATC 3 cut(s) 991, 1003, 1087
BceAI ACGGC 1 cut(s) 1149
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciT130I CCWGG 1 cut(s) 536
BclI TGATCA 2 cut(s) 210, 321
BcoDI GTCTC 2 cut(s) 96, 233
BfoI RGCGCY 1 cut(s) 767
BfuAI ACCTGC 2 cut(s) 502, 815
BglI GCCNNNNNGGC 1 cut(s) 379
BisI GCNGC 6 cut(s) 146, 169, 411, 414, 417, 934
BlsI GCNGC 6 cut(s) 147, 170, 412, 415, 418, 935
Bme1390I CCNGG 1 cut(s) 536
Bme18I GGWCC 2 cut(s) 315, 491
BmgT120I GGNCC 3 cut(s) 315, 380, 491
BmiI GGNNCC 3 cut(s) 316, 747, 852
BmrFI CCNGG 1 cut(s) 536
BmsI GCATC 6 cut(s) 253, 337, 378, 967, 1072, 1091
BplI GAGNNNNNCTC 2 cut(s) 439, 471
BpmI CTGGAG 1 cut(s) 323
Bpu14I TTCGAA 2 cut(s) 549, 1052
BpuEI CTTGAG 2 cut(s) 233, 678
Bsa29I ATCGAT 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 395
BsaI GGTCTC 1 cut(s) 233
BsaJI CCNNGG 2 cut(s) 535, 884
BsaWI WCCGGW 2 cut(s) 188, 748
BsaXI ACNNNNNCTCC 4 cut(s) 258, 288, 964, 994
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 224
Bse1I ACTGG 2 cut(s) 653, 967
Bse3DI GCAATG 1 cut(s) 943
BseAI TCCGGA 1 cut(s) 748
BseBI CCWGG 1 cut(s) 536
BseCI ATCGAT 1 cut(s) 162
BseDI CCNNGG 2 cut(s) 535, 884
BseGI GGATG 3 cut(s) 586, 775, 876
BseLI CCNNNNNNNGG 2 cut(s) 128, 224
BseMI GCAATG 1 cut(s) 943
BseMII CTCAG 3 cut(s) 212, 235, 470
BseNI ACTGG 2 cut(s) 653, 967
BseRI GAGGAG 4 cut(s) 1073, 1076, 1087, 1163
BseSI GKGCMC 1 cut(s) 672
BseXI GCAGC 5 cut(s) 157, 400, 403, 422, 945
BsgI GTGCAG 1 cut(s) 480
Bsh1236I CGCG 1 cut(s) 502
Bsh1285I CGRYCG 2 cut(s) 180, 1121
BshFI GGCC 2 cut(s) 373, 382
BshNI GGYRCC 1 cut(s) 850
BshVI ATCGAT 1 cut(s) 162
BsiEI CGRYCG 2 cut(s) 180, 1121
BsiHKAI GWGCWC 4 cut(s) 672, 786, 882, 997
BsiSI CCGG 4 cut(s) 189, 383, 398, 749
BslI CCNNNNNNNGG 2 cut(s) 128, 224
BsmAI GTCTC 2 cut(s) 96, 233
BsnI GGCC 2 cut(s) 373, 382
Bso31I GGTCTC 1 cut(s) 233
Bsp119I TTCGAA 2 cut(s) 549, 1052
Bsp1286I GDGCHC 4 cut(s) 672, 786, 882, 997
Bsp13I TCCGGA 1 cut(s) 748
Bsp143I GATC 9 cut(s) 40, 82, 159, 210, 321, 541, 564, 745, 1118
BspACI CCGC 7 cut(s) 86, 92, 168, 176, 440, 500, 757
BspANI GGCC 2 cut(s) 373, 382
BspCNI CTCAG 3 cut(s) 211, 234, 469
BspDI ATCGAT 1 cut(s) 162
BspEI TCCGGA 1 cut(s) 748
BspFNI CGCG 1 cut(s) 502
BspHI TCATGA 1 cut(s) 43
BspLI GGNNCC 3 cut(s) 316, 747, 852
BspMI ACCTGC 2 cut(s) 502, 815
BspPI GGATC 4 cut(s) 77, 572, 740, 753
BspT104I TTCGAA 2 cut(s) 549, 1052
BspT107I GGYRCC 1 cut(s) 850
BspTNI GGTCTC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 943
BsrI ACTGG 2 cut(s) 653, 967
BssECI CCNNGG 2 cut(s) 535, 884
BssMI GATC 9 cut(s) 40, 82, 159, 210, 321, 541, 564, 745, 1118
BssNI GRCGYC 1 cut(s) 395
Bst2UI CCWGG 1 cut(s) 536
Bst6I CTCTTC 6 cut(s) 29, 236, 546, 853, 1064, 1166
BstACI GRCGYC 1 cut(s) 395
BstAPI GCANNNNNTGC 2 cut(s) 151, 416
BstBI TTCGAA 2 cut(s) 549, 1052
BstC8I GCNNGC 5 cut(s) 90, 242, 700, 1047, 1162
BstDEI CTNAG 4 cut(s) 198, 221, 456, 1005
BstDSI CCRYGG 1 cut(s) 884
BstENI CCTNNNNNAGG 1 cut(s) 222
BstF5I GGATG 3 cut(s) 586, 775, 876
BstFNI CGCG 1 cut(s) 502
BstH2I RGCGCY 1 cut(s) 767
BstHHI GCGC 1 cut(s) 766
BstKTI GATC 9 cut(s) 43, 85, 162, 213, 324, 544, 567, 748, 1121
BstMAI GTCTC 2 cut(s) 96, 233
BstMBI GATC 9 cut(s) 40, 82, 159, 210, 321, 541, 564, 745, 1118
BstMCI CGRYCG 2 cut(s) 180, 1121
BstNI CCWGG 1 cut(s) 536
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 1 cut(s) 534
BstSLI GKGCMC 1 cut(s) 672
BstUI CGCG 1 cut(s) 502
BstV1I GCAGC 5 cut(s) 157, 400, 403, 422, 945
BstX2I RGATCY 1 cut(s) 745
BstYI RGATCY 1 cut(s) 745
Bsu15I ATCGAT 1 cut(s) 162
BsuRI GGCC 2 cut(s) 373, 382
BsuTUI ATCGAT 1 cut(s) 162
BtgI CCRYGG 1 cut(s) 884
BtgZI GCGATG 1 cut(s) 1026
BtsCI GGATG 3 cut(s) 586, 775, 876
BveI ACCTGC 2 cut(s) 502, 815
Cac8I GCNNGC 5 cut(s) 90, 242, 700, 1047, 1162
CaiI CAGNNNCTG 1 cut(s) 416
CciI TCATGA 1 cut(s) 43
CfoI GCGC 1 cut(s) 766
Cfr13I GGNCC 3 cut(s) 315, 380, 491
ClaI ATCGAT 1 cut(s) 162
CseI GACGC 1 cut(s) 403
Csp6I GTAC 2 cut(s) 646, 909
CviAII CATG 6 cut(s) 44, 287, 319, 568, 820, 899
CviQI GTAC 2 cut(s) 646, 909
DdeI CTNAG 4 cut(s) 198, 221, 456, 1005
DpnI GATC 9 cut(s) 42, 84, 161, 212, 323, 543, 566, 747, 1120
DpnII GATC 9 cut(s) 40, 82, 159, 210, 321, 541, 564, 745, 1118
DraIII CACNNNGTG 2 cut(s) 503, 619
Eam1104I CTCTTC 6 cut(s) 29, 236, 546, 853, 1064, 1166
EarI CTCTTC 6 cut(s) 29, 236, 546, 853, 1064, 1166
EciI GGCGGA 1 cut(s) 455
Ecl136II GAGCTC 3 cut(s) 784, 880, 995
Eco24I GRGCYC 3 cut(s) 786, 882, 997
Eco31I GGTCTC 1 cut(s) 233
Eco47I GGWCC 2 cut(s) 315, 491
Eco47III AGCGCT 1 cut(s) 765
Eco53kI GAGCTC 3 cut(s) 784, 880, 995
Eco57I CTGAAG 1 cut(s) 537
EcoICRI GAGCTC 3 cut(s) 784, 880, 995
EcoNI CCTNNNNNAGG 1 cut(s) 222
EcoO109I RGGNCCY 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 534
EcoT38I GRGCYC 3 cut(s) 786, 882, 997
FaeI CATG 6 cut(s) 47, 290, 322, 571, 823, 902
FatI CATG 6 cut(s) 43, 286, 318, 567, 819, 898
FbaI TGATCA 2 cut(s) 210, 321
FblI GTMKAC 2 cut(s) 609, 1200
Fnu4HI GCNGC 6 cut(s) 146, 169, 411, 414, 417, 934
FokI GGATG 3 cut(s) 593, 782, 883
FriOI GRGCYC 3 cut(s) 786, 882, 997
Fsp4HI GCNGC 6 cut(s) 146, 169, 411, 414, 417, 934
GlaI GCGC 1 cut(s) 765
GluI GCNGC 6 cut(s) 146, 169, 411, 414, 417, 934
GsuI CTGGAG 1 cut(s) 323
HaeII RGCGCY 1 cut(s) 767
HaeIII GGCC 2 cut(s) 373, 382
HapII CCGG 4 cut(s) 189, 383, 398, 749
HgaI GACGC 1 cut(s) 403
HhaI GCGC 1 cut(s) 766
Hin1I GRCGYC 1 cut(s) 395
Hin1II CATG 6 cut(s) 47, 290, 322, 571, 823, 902
Hin6I GCGC 1 cut(s) 764
HinP1I GCGC 1 cut(s) 764
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 4 cut(s) 59, 65, 524, 804
HpaII CCGG 4 cut(s) 189, 383, 398, 749
HphI GGTGA 4 cut(s) 92, 551, 568, 839
Hpy166II GTNNAC 4 cut(s) 16, 610, 670, 1201
Hpy188I TCNGA 7 cut(s) 64, 123, 201, 210, 224, 988, 999
Hpy188III TCNNGA 4 cut(s) 44, 302, 637, 749
Hpy8I GTNNAC 4 cut(s) 16, 610, 670, 1201
Hpy99I CGWCG 1 cut(s) 397
HpyAV CCTTC 4 cut(s) 106, 363, 941, 994
HpyCH4V TGCA 8 cut(s) 154, 244, 410, 497, 670, 916, 1085, 1104
HpyF3I CTNAG 4 cut(s) 198, 221, 456, 1005
Hsp92I GRCGYC 1 cut(s) 395
Hsp92II CATG 6 cut(s) 47, 290, 322, 571, 823, 902
HspAI GCGC 1 cut(s) 764
Kpn2I TCCGGA 1 cut(s) 748
Ksp22I TGATCA 2 cut(s) 210, 321
Kzo9I GATC 9 cut(s) 40, 82, 159, 210, 321, 541, 564, 745, 1118
LmnI GCTCC 4 cut(s) 266, 400, 885, 985
Lsp1109I GCAGC 5 cut(s) 157, 400, 403, 422, 945
LweI GCATC 6 cut(s) 253, 337, 378, 967, 1072, 1091
MaeIII GTNAC 3 cut(s) 556, 738, 1131
MalI GATC 9 cut(s) 42, 84, 161, 212, 323, 543, 566, 747, 1120
MboI GATC 9 cut(s) 40, 82, 159, 210, 321, 541, 564, 745, 1118
MfeI CAATTG 2 cut(s) 155, 375
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 4 cut(s) 672, 786, 882, 997
MluCI AATT 4 cut(s) 155, 375, 422, 443
MmeI TCCRAC 3 cut(s) 320, 441, 950
MroI TCCGGA 1 cut(s) 748
MseI TTAA 4 cut(s) 51, 732, 843, 1019
MspA1I CMGCKG 1 cut(s) 413
MspI CCGG 4 cut(s) 189, 383, 398, 749
MspR9I CCNGG 1 cut(s) 536
MunI CAATTG 2 cut(s) 155, 375
MvaI CCWGG 1 cut(s) 536
MvnI CGCG 1 cut(s) 502
NdeII GATC 9 cut(s) 40, 82, 159, 210, 321, 541, 564, 745, 1118
NlaIII CATG 6 cut(s) 47, 290, 322, 571, 823, 902
NlaIV GGNNCC 3 cut(s) 316, 747, 852
NmuCI GTSAC 1 cut(s) 556
NspI RCATGY 1 cut(s) 290
NspV TTCGAA 2 cut(s) 549, 1052
PagI TCATGA 1 cut(s) 43
PaqCI CACCTGC 1 cut(s) 815
PciI ACATGT 1 cut(s) 286
PcsI WCGNNNNNNNCGW 2 cut(s) 270, 1049
PfeI GAWTC 4 cut(s) 59, 65, 524, 804
PkrI GCNGC 6 cut(s) 147, 170, 412, 415, 418, 935
Ple19I CGATCG 1 cut(s) 1121
PpuMI RGGWCCY 1 cut(s) 491
PscI ACATGT 1 cut(s) 286
PshBI ATTAAT 1 cut(s) 51
Psp124BI GAGCTC 3 cut(s) 786, 882, 997
Psp5II RGGWCCY 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 534
PspGI CCWGG 1 cut(s) 534
PspN4I GGNNCC 3 cut(s) 316, 747, 852
PspPI GGNCC 3 cut(s) 315, 380, 491
PspPPI RGGWCCY 1 cut(s) 491
PstNI CAGNNNCTG 1 cut(s) 416
PsuI RGATCY 1 cut(s) 745
PvuI CGATCG 1 cut(s) 1121
PvuII CAGCTG 1 cut(s) 413
RsaI GTAC 2 cut(s) 647, 910
RsaNI GTAC 2 cut(s) 646, 909
SacI GAGCTC 3 cut(s) 786, 882, 997
SaqAI TTAA 4 cut(s) 51, 732, 843, 1019
SatI GCNGC 6 cut(s) 146, 169, 411, 414, 417, 934
Sau3AI GATC 9 cut(s) 40, 82, 159, 210, 321, 541, 564, 745, 1118
Sau96I GGNCC 3 cut(s) 315, 380, 491
ScrFI CCNGG 1 cut(s) 536
SduI GDGCHC 4 cut(s) 672, 786, 882, 997
SfaNI GCATC 6 cut(s) 253, 337, 378, 967, 1072, 1091
SfiI GGCCNNNNNGGCC 1 cut(s) 379
SfuI TTCGAA 2 cut(s) 549, 1052
SinI GGWCC 2 cut(s) 315, 491
SmlI CTYRAG 2 cut(s) 248, 693
SmoI CTYRAG 2 cut(s) 248, 693
Sse9I AATT 4 cut(s) 155, 375, 422, 443
SsiI CCGC 7 cut(s) 86, 92, 168, 176, 440, 500, 757
SspI AATATT 1 cut(s) 127
SstI GAGCTC 3 cut(s) 786, 882, 997
StyD4I CCNGG 1 cut(s) 534
TaqI TCGA 7 cut(s) 162, 180, 352, 549, 920, 1052, 1121
TasI AATT 4 cut(s) 155, 375, 422, 443
TatI WGTACW 1 cut(s) 908
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 4 cut(s) 59, 65, 524, 804
Tru1I TTAA 4 cut(s) 51, 732, 843, 1019
Tru9I TTAA 4 cut(s) 51, 732, 843, 1019
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 5 cut(s) 145, 410, 413, 416, 933
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 2 cut(s) 17, 1029
TspGWI ACGGA 1 cut(s) 279
VneI GTGCAC 1 cut(s) 668
VpaK11BI GGWCC 2 cut(s) 315, 491
VspI ATTAAT 1 cut(s) 51
XagI CCTNNNNNAGG 1 cut(s) 222
XceI RCATGY 1 cut(s) 290
XcmI CCANNNNNNNNNTGG 1 cut(s) 1192
XmiI GTMKAC 2 cut(s) 609, 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.