Rw5G049400

Protein of unknown function (DUF3752)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001274
Physical Location & Seq
Forward (+)
85641977 .. 85645104
3128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G049400.1

Sequence Viewer

Length: 1314 bp
ATGAAATCGAAAGACAACGATAAGAAGAAGCAGAGAGCTTATGAGATTGATGATGATTCTGGGTCTTCGAGCGGTGGTGATCCGCAAGCGGTTGTGCGAGACTTGTTGAAGGAATTTCCTAATATTGGGTGCGATTTGAAGCAGCTTTTGCAAGTGATCGATGGGGGCAAAGCGGTTGACATAAATGGACTAGCTCAGAGACCTCTGATCAAGCCTCTCAGGACTCTGTTCCTTTGCTTGAATCTCAAGGAACTGAATCACGGAGGTTTTGTATTGCCTCCAAATGTTCGTCCAACTTTGGAGGTTGTTGGTTCCATGATTACGCCTTCTTTAGATAATGTTGTTCCTCCTGAGAGAAGGCCAATTGGCCCGGAGAAGCCGTCGCCGGAGATACTTGATGCTGCAGCCAAATTGAGGGAGGCAGAGGCCGAGTTAAGGGAAGCGTTGGAAGAAAAAGATGATGGGGTTTTTATTGGACCTGCACCACGTGCTGTGGCGGCTGAAGCTGAATCAGCTAACCAGGCTGACCGGTTTGAAGAGGTGACAAGGATCACGGAAGCAGAGGATGATTGCCCCTATGAGATACTAGGATCAAACCACAATGTGTCTGCTGATAGCATCAAGAAAAGGTACTGGAAGTTGTCACTTTTGGTGCACCCTGATAAATGCTCACACCCTCAAGCCAACCAAGCATTTGTGAAGCTGAACAAGGCCTTTAAAGAGCTACAGGATCCGCAGAAGCGGAGAGCACTGGATGAGAAGATTGAGCTCAGGGAGAAGCAGGAGAGATTCATTCTACTGGCAGGTGATGTGAGTGGTGTTAAAGCGGCACGAAAAAGAGATGAGTGGATGACAGAGCTTCCACCAGAGAGGAAACATGGTATGAGTCCAAGGCAGTCGACAATGTTTAGCAGGAGTGCTAAACAAGGGCGTGGGGATACCAGTGGATGGACTGATGCTCCTTCTGAAAGAGCTTGGAAAGCTAAGATGGAGTACTTACAAGCCTATAATGAGTTTGCATCACTTGCTTCGAATGAGGAGGAGAGGAAGAGGAGTTTAGTGGCAGAGTTGATGGACAATTACAAGAAAGATAAACGATCGAAATCATTGCTGCAAAAGCACCAAGATGAGATTAGAGCAAAGAGAAAATACTCCAAGCAACAGGCAGAGGAGGAAGACTGGAACTGTTGCAGGTACCAAAAAGCGCAATGGATGGTAGAAGGATATGAACAGCAGATACATAATTCGTTTTATTTCCTTTCTGATCCCATCACTCCAAACAACAGACAGAAGAATACTACAGAATATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001664 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0050780
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3752 DnaJ

Protein Analysis

437

Amino Acids

49.81

Weight (kDa)

6.71

Isoelectric Point (pI)

57.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 192 - 252 9e-18 DnaJ domain
DUF3752 PF12572 288 - 394 2.1e-13 Protein of unknown function (DUF3752)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 794
Acc36I ACCTGC 3 cut(s) 487, 794, 1182
Acc65I GGTACC 1 cut(s) 1194
AccB1I GGYRCC 1 cut(s) 1194
AccB7I CCANNNNNTGG 1 cut(s) 948
AccBSI CCGCTC 1 cut(s) 72
AccI GTMKAC 1 cut(s) 899
AciI CCGC 8 cut(s) 72, 83, 89, 173, 497, 734, 742, 827
AclWI GGATC 6 cut(s) 74, 557, 598, 725, 738, 1259
AcsI RAATTY 1 cut(s) 113
AcuI CTGAAG 1 cut(s) 522
AcvI CACGTG 1 cut(s) 488
AdeI CACNNNGTG 2 cut(s) 488, 604
AfaI GTAC 3 cut(s) 632, 995, 1196
AfiI CCNNNNNNNGG 4 cut(s) 125, 414, 435, 948
AgeI ACCGGT 1 cut(s) 528
AgsI TTSAA 4 cut(s) 109, 139, 241, 536
AjnI CCWGG 1 cut(s) 519
Alw21I GWGCWC 3 cut(s) 657, 751, 771
Alw26I GTCTC 2 cut(s) 93, 193
Alw44I GTGCAC 1 cut(s) 653
AlwI GGATC 6 cut(s) 74, 557, 598, 725, 738, 1259
AoxI GGCC 4 cut(s) 359, 367, 426, 711
ApaLI GTGCAC 1 cut(s) 653
ApeKI GCWGC 4 cut(s) 142, 401, 404, 1111
ApoI RAATTY 1 cut(s) 113
AsiGI ACCGGT 1 cut(s) 528
Asp718I GGTACC 1 cut(s) 1194
AspLEI GCGC 1 cut(s) 1207
AspS9I GGNCC 2 cut(s) 368, 476
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 3 cut(s) 89, 553, 818
AsuII TTCGAA 1 cut(s) 1031
AvaII GGWCC 1 cut(s) 476
BaeGI GKGCMC 1 cut(s) 657
BamHI GGATCC 1 cut(s) 730
BanI GGYRCC 1 cut(s) 1194
BanII GRGCYC 1 cut(s) 771
BbrPI CACGTG 1 cut(s) 488
BbsI GAAGAC 2 cut(s) 57, 1182
Bbv12I GWGCWC 3 cut(s) 657, 751, 771
BbvI GCAGC 4 cut(s) 154, 388, 416, 1098
BccI CCATC 7 cut(s) 155, 455, 942, 982, 1066, 1207, 1277
BceAI ACGGC 1 cut(s) 364
BcgI CGANNNNNNTGC 2 cut(s) 1090, 1124
BciT130I CCWGG 1 cut(s) 521
BciVI GTATCC 1 cut(s) 931
BclI TGATCA 1 cut(s) 207
BcnI CCSGG 1 cut(s) 371
BcoDI GTCTC 2 cut(s) 93, 193
BfaI CTAG 2 cut(s) 191, 587
BfmI CTRYAG 3 cut(s) 402, 725, 1299
BfuAI ACCTGC 3 cut(s) 487, 794, 1182
BfuI GTATCC 1 cut(s) 931
BisI GCNGC 6 cut(s) 143, 402, 405, 498, 828, 1112
BlsI GCNGC 6 cut(s) 144, 403, 406, 499, 829, 1113
BmcAI AGTACT 1 cut(s) 995
Bme1390I CCNGG 2 cut(s) 371, 521
Bme18I GGWCC 1 cut(s) 476
BmgT120I GGNCC 2 cut(s) 368, 476
BmiI GGNNCC 3 cut(s) 313, 732, 1196
BmrFI CCNGG 2 cut(s) 371, 521
BmsI GCATC 4 cut(s) 388, 627, 946, 1028
BpiI GAAGAC 2 cut(s) 57, 1182
Bpu10I CCTNAGC 1 cut(s) 770
Bpu14I TTCGAA 1 cut(s) 1031
BpuEI CTTGAG 2 cut(s) 230, 663
BpuMI CCSGG 1 cut(s) 371
Bsa29I ATCGAT 1 cut(s) 159
BsaAI YACGTR 1 cut(s) 488
BsaBI GATNNNNATC 1 cut(s) 1102
BsaI GGTCTC 1 cut(s) 193
BsaJI CCNNGG 1 cut(s) 890
BsaWI WCCGGW 1 cut(s) 528
BsaXI ACNNNNNCTCC 6 cut(s) 255, 285, 365, 395, 943, 973
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 414, 435, 948
Bse118I RCCGGY 1 cut(s) 528
Bse1I ACTGG 5 cut(s) 638, 756, 804, 942, 1184
Bse3DI GCAATG 2 cut(s) 1106, 1214
Bse8I GATNNNNATC 1 cut(s) 1102
BseBI CCWGG 1 cut(s) 521
BseCI ATCGAT 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 890
BseGI GGATG 5 cut(s) 571, 760, 855, 953, 1218
BseJI GATNNNNATC 1 cut(s) 1102
BseLI CCNNNNNNNGG 4 cut(s) 125, 414, 435, 948
BseMI GCAATG 2 cut(s) 1106, 1214
BseMII CTCAG 4 cut(s) 209, 232, 342, 784
BseNI ACTGG 5 cut(s) 638, 756, 804, 942, 1184
BseRI GAGGAG 4 cut(s) 1052, 1055, 1066, 1184
BseSI GKGCMC 1 cut(s) 657
BseXI GCAGC 4 cut(s) 154, 388, 416, 1098
BsgI GTGCAG 1 cut(s) 465
Bsh1285I CGRYCG 1 cut(s) 1100
BshFI GGCC 4 cut(s) 361, 369, 428, 713
BshNI GGYRCC 1 cut(s) 1194
BshTI ACCGGT 1 cut(s) 528
BshVI ATCGAT 1 cut(s) 159
BsiEI CGRYCG 1 cut(s) 1100
BsiHKAI GWGCWC 3 cut(s) 657, 751, 771
BsiSI CCGG 3 cut(s) 371, 386, 529
BslI CCNNNNNNNGG 4 cut(s) 125, 414, 435, 948
BsmAI GTCTC 2 cut(s) 93, 193
BsnI GGCC 4 cut(s) 361, 369, 428, 713
Bso31I GGTCTC 1 cut(s) 193
Bsp119I TTCGAA 1 cut(s) 1031
Bsp1286I GDGCHC 3 cut(s) 657, 751, 771
Bsp143I GATC 8 cut(s) 79, 156, 207, 549, 590, 730, 1097, 1264
BspACI CCGC 8 cut(s) 72, 83, 89, 173, 497, 734, 742, 827
BspANI GGCC 4 cut(s) 361, 369, 428, 713
BspCNI CTCAG 4 cut(s) 208, 231, 343, 783
BspDI ATCGAT 1 cut(s) 159
BspLI GGNNCC 3 cut(s) 313, 732, 1196
BspMAI CTGCAG 1 cut(s) 406
BspMI ACCTGC 3 cut(s) 487, 794, 1182
BspPI GGATC 6 cut(s) 74, 557, 598, 725, 738, 1259
BspT104I TTCGAA 1 cut(s) 1031
BspT107I GGYRCC 1 cut(s) 1194
BspTNI GGTCTC 1 cut(s) 193
BsrBI CCGCTC 1 cut(s) 72
BsrDI GCAATG 2 cut(s) 1106, 1214
BsrFI RCCGGY 1 cut(s) 528
BsrI ACTGG 5 cut(s) 638, 756, 804, 942, 1184
BssAI RCCGGY 1 cut(s) 528
BssECI CCNNGG 1 cut(s) 890
BssMI GATC 8 cut(s) 79, 156, 207, 549, 590, 730, 1097, 1264
BssT1I CCWWGG 1 cut(s) 890
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 1 cut(s) 1187
Bst6I CTCTTC 2 cut(s) 531, 1043
BstAPI GCANNNNNTGC 3 cut(s) 148, 488, 1025
BstBAI YACGTR 1 cut(s) 488
BstBI TTCGAA 1 cut(s) 1031
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 5 cut(s) 195, 218, 351, 770, 984
BstF5I GGATG 5 cut(s) 571, 760, 855, 953, 1218
BstHHI GCGC 1 cut(s) 1207
BstKTI GATC 8 cut(s) 82, 159, 210, 552, 593, 733, 1100, 1267
BstMAI GTCTC 2 cut(s) 93, 193
BstMBI GATC 8 cut(s) 79, 156, 207, 549, 590, 730, 1097, 1264
BstMCI CGRYCG 1 cut(s) 1100
BstNI CCWGG 1 cut(s) 521
BstSCI CCNGG 2 cut(s) 369, 519
BstSFI CTRYAG 3 cut(s) 402, 725, 1299
BstSLI GKGCMC 1 cut(s) 657
BstV1I GCAGC 4 cut(s) 154, 388, 416, 1098
BstV2I GAAGAC 2 cut(s) 57, 1182
BstX2I RGATCY 1 cut(s) 730
BstYI RGATCY 1 cut(s) 730
Bsu15I ATCGAT 1 cut(s) 159
BsuI GTATCC 1 cut(s) 931
BsuRI GGCC 4 cut(s) 361, 369, 428, 713
BsuTUI ATCGAT 1 cut(s) 159
BtsCI GGATG 5 cut(s) 571, 760, 855, 953, 1218
BtsIMutI CAGTG 2 cut(s) 749, 949
BveI ACCTGC 3 cut(s) 487, 794, 1182
Cac8I GCNNGC 1 cut(s) 87
CfoI GCGC 1 cut(s) 1207
Cfr10I RCCGGY 1 cut(s) 528
Cfr13I GGNCC 2 cut(s) 368, 476
ClaI ATCGAT 1 cut(s) 159
Csp6I GTAC 3 cut(s) 631, 994, 1195
CspAI ACCGGT 1 cut(s) 528
CviAII CATG 2 cut(s) 316, 878
CviQI GTAC 3 cut(s) 631, 994, 1195
DdeI CTNAG 5 cut(s) 195, 218, 351, 770, 984
DpnI GATC 8 cut(s) 81, 158, 209, 551, 592, 732, 1099, 1266
DpnII GATC 8 cut(s) 79, 156, 207, 549, 590, 730, 1097, 1264
DraI TTTAAA 1 cut(s) 718
DraIII CACNNNGTG 2 cut(s) 488, 604
Eam1104I CTCTTC 2 cut(s) 531, 1043
EarI CTCTTC 2 cut(s) 531, 1043
Ecl136II GAGCTC 1 cut(s) 769
Eco130I CCWWGG 1 cut(s) 890
Eco147I AGGCCT 1 cut(s) 713
Eco24I GRGCYC 1 cut(s) 771
Eco31I GGTCTC 1 cut(s) 193
Eco47I GGWCC 1 cut(s) 476
Eco53kI GAGCTC 1 cut(s) 769
Eco57I CTGAAG 1 cut(s) 522
Eco72I CACGTG 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 769
EcoRII CCWGG 1 cut(s) 519
EcoT14I CCWWGG 1 cut(s) 890
EcoT38I GRGCYC 1 cut(s) 771
ErhI CCWWGG 1 cut(s) 890
FaeI CATG 2 cut(s) 319, 881
FaiI YATR 9 cut(s) 42, 182, 317, 579, 879, 884, 1008, 1227, 1242
FatI CATG 2 cut(s) 315, 877
FbaI TGATCA 1 cut(s) 207
FblI GTMKAC 1 cut(s) 899
Fnu4HI GCNGC 6 cut(s) 143, 402, 405, 498, 828, 1112
FokI GGATG 5 cut(s) 578, 767, 862, 960, 1225
FriOI GRGCYC 1 cut(s) 771
Fsp4HI GCNGC 6 cut(s) 143, 402, 405, 498, 828, 1112
FspBI CTAG 2 cut(s) 191, 587
GlaI GCGC 1 cut(s) 1206
GluI GCNGC 6 cut(s) 143, 402, 405, 498, 828, 1112
HaeIII GGCC 4 cut(s) 361, 369, 428, 713
HapII CCGG 3 cut(s) 371, 386, 529
HhaI GCGC 1 cut(s) 1207
Hin1II CATG 2 cut(s) 319, 881
Hin6I GCGC 1 cut(s) 1205
HinP1I GCGC 1 cut(s) 1205
HincII GTYRAC 2 cut(s) 178, 900
HindII GTYRAC 2 cut(s) 178, 900
HinfI GANTC 7 cut(s) 56, 223, 241, 256, 509, 789, 886
HpaII CCGG 3 cut(s) 371, 386, 529
HphI GGTGA 3 cut(s) 89, 553, 818
Hpy166II GTNNAC 3 cut(s) 178, 655, 900
Hpy188I TCNGA 4 cut(s) 198, 207, 967, 1264
Hpy188III TCNNGA 3 cut(s) 220, 350, 622
Hpy8I GTNNAC 3 cut(s) 178, 655, 900
Hpy99I CGWCG 1 cut(s) 385
HpyAV CCTTC 5 cut(s) 103, 336, 351, 972, 1214
HpyCH4III ACNGT 1 cut(s) 1187
HpyCH4IV ACGT 1 cut(s) 487
HpyCH4V TGCA 7 cut(s) 151, 404, 482, 655, 1019, 1114, 1191
HpyF3I CTNAG 5 cut(s) 195, 218, 351, 770, 984
HpySE526I ACGT 1 cut(s) 487
Hsp92II CATG 2 cut(s) 319, 881
HspAI GCGC 1 cut(s) 1205
KpnI GGTACC 1 cut(s) 1198
Ksp22I TGATCA 1 cut(s) 207
Kzo9I GATC 8 cut(s) 79, 156, 207, 549, 590, 730, 1097, 1264
LmnI GCTCC 1 cut(s) 964
Lsp1109I GCAGC 4 cut(s) 154, 388, 416, 1098
LweI GCATC 4 cut(s) 388, 627, 946, 1028
MaeI CTAG 2 cut(s) 191, 587
MaeII ACGT 1 cut(s) 487
MaeIII GTNAC 2 cut(s) 541, 642
MalI GATC 8 cut(s) 81, 158, 209, 551, 592, 732, 1099, 1266
MbiI CCGCTC 1 cut(s) 72
MboI GATC 8 cut(s) 79, 156, 207, 549, 590, 730, 1097, 1264
MboII GAAGA 8 cut(s) 37, 57, 461, 548, 772, 1060, 1187, 1303
MfeI CAATTG 1 cut(s) 363
MflI RGATCY 1 cut(s) 730
MhlI GDGCHC 3 cut(s) 657, 751, 771
MluCI AATT 5 cut(s) 113, 363, 410, 1078, 1243
MlyI GAGTC 2 cut(s) 217, 895
MmeI TCCRAC 2 cut(s) 317, 426
MseI TTAA 3 cut(s) 434, 717, 822
MslI CAYNNNNRTG 1 cut(s) 1125
MspI CCGG 3 cut(s) 371, 386, 529
MspR9I CCNGG 2 cut(s) 371, 521
MunI CAATTG 1 cut(s) 363
MvaI CCWGG 1 cut(s) 521
NciI CCSGG 1 cut(s) 371
NdeII GATC 8 cut(s) 79, 156, 207, 549, 590, 730, 1097, 1264
NlaIII CATG 2 cut(s) 319, 881
NlaIV GGNNCC 3 cut(s) 313, 732, 1196
NmeAIII GCCGAG 1 cut(s) 454
NmuCI GTSAC 2 cut(s) 541, 642
NspV TTCGAA 1 cut(s) 1031
PaqCI CACCTGC 1 cut(s) 794
PceI AGGCCT 1 cut(s) 713
PfeI GAWTC 5 cut(s) 56, 241, 256, 509, 789
PflMI CCANNNNNTGG 1 cut(s) 948
PinAI ACCGGT 1 cut(s) 528
PkrI GCNGC 6 cut(s) 144, 403, 406, 499, 829, 1113
Ple19I CGATCG 1 cut(s) 1100
PleI GAGTC 2 cut(s) 217, 894
PmaCI CACGTG 1 cut(s) 488
PmlI CACGTG 1 cut(s) 488
PpsI GAGTC 2 cut(s) 217, 894
Ppu21I YACGTR 1 cut(s) 488
Psp124BI GAGCTC 1 cut(s) 771
Psp6I CCWGG 1 cut(s) 519
PspCI CACGTG 1 cut(s) 488
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 3 cut(s) 313, 732, 1196
PspPI GGNCC 2 cut(s) 368, 476
PsrI GAACNNNNNNTAC 2 cut(s) 1221, 1253
PstI CTGCAG 1 cut(s) 406
PsuI RGATCY 1 cut(s) 730
PvuI CGATCG 1 cut(s) 1100
RsaI GTAC 3 cut(s) 632, 995, 1196
RsaNI GTAC 3 cut(s) 631, 994, 1195
RseI CAYNNNNRTG 1 cut(s) 1125
SacI GAGCTC 1 cut(s) 771
SalI GTCGAC 1 cut(s) 898
SaqAI TTAA 3 cut(s) 434, 717, 822
SatI GCNGC 6 cut(s) 143, 402, 405, 498, 828, 1112
Sau3AI GATC 8 cut(s) 79, 156, 207, 549, 590, 730, 1097, 1264
Sau96I GGNCC 2 cut(s) 368, 476
ScaI AGTACT 1 cut(s) 995
SchI GAGTC 2 cut(s) 217, 895
ScrFI CCNGG 2 cut(s) 371, 521
SduI GDGCHC 3 cut(s) 657, 751, 771
SfaNI GCATC 4 cut(s) 388, 627, 946, 1028
SfcI CTRYAG 3 cut(s) 402, 725, 1299
SfuI TTCGAA 1 cut(s) 1031
SinI GGWCC 1 cut(s) 476
SmiMI CAYNNNNRTG 1 cut(s) 1125
SmlI CTYRAG 2 cut(s) 245, 678
SmoI CTYRAG 2 cut(s) 245, 678
Sse9I AATT 5 cut(s) 113, 363, 410, 1078, 1243
SseBI AGGCCT 1 cut(s) 713
SsiI CCGC 8 cut(s) 72, 83, 89, 173, 497, 734, 742, 827
SspI AATATT 2 cut(s) 124, 1307
SspMI CTAG 2 cut(s) 191, 587
SstI GAGCTC 1 cut(s) 771
StuI AGGCCT 1 cut(s) 713
StyD4I CCNGG 2 cut(s) 369, 519
StyI CCWWGG 1 cut(s) 890
TaaI ACNGT 1 cut(s) 1187
TaiI ACGT 1 cut(s) 490
TaqI TCGA 6 cut(s) 8, 68, 159, 899, 1031, 1100
TasI AATT 5 cut(s) 113, 363, 410, 1078, 1243
TatI WGTACW 1 cut(s) 993
TauI GCSGC 2 cut(s) 500, 830
TfiI GAWTC 5 cut(s) 56, 241, 256, 509, 789
Tru1I TTAA 3 cut(s) 434, 717, 822
Tru9I TTAA 3 cut(s) 434, 717, 822
TscAI CASTG 2 cut(s) 756, 949
TseFI GTSAC 2 cut(s) 541, 642
TseI GCWGC 4 cut(s) 142, 401, 404, 1111
Tsp45I GTSAC 2 cut(s) 541, 642
TspDTI ATGAA 3 cut(s) 17, 781, 1242
TspGWI ACGGA 2 cut(s) 276, 569
TspRI CASTG 2 cut(s) 756, 949
Van91I CCANNNNNTGG 1 cut(s) 948
VneI GTGCAC 1 cut(s) 653
VpaK11BI GGWCC 1 cut(s) 476
XapI RAATTY 1 cut(s) 113
XmiI GTMKAC 1 cut(s) 899
XspI CTAG 2 cut(s) 191, 587
ZrmI AGTACT 1 cut(s) 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.