Rh4BG082100

Protein of unknown function (DUF3752)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001274
Physical Location & Seq
Reverse (-)
14310229 .. 14319904
9676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG082100.1

Sequence Viewer

Length: 1554 bp
ATGAAATGTGAACGGGCTGGTTGGACCCTAGAAGTGGGCCTCAACTTGTCCTATATTCATGATGTTGGGCCTTTAGCGTGTACCAAAACCCAAAACTTGGATTTATTAGAGGTGTGGTTTCAACAGTGCTCTGAATTTGATTGCAAAATGGGGAGTAAAAGCAAGAGGAAGAAGCAATTGCCGGAATCGAGCGACGATGATTCACCGTCGTCGTCTCCTTCAAGCTCTGACGATTCCGACCGCTCCTCGAAACGGCGTCGTCGCCACCGTCACAGAAGCGACGACGGCTCCAGAAGAGAGAAAGAAAGGAGAAGGGAGAGGAGAGAGAAACGGCGAAGTAAGAGAGAGGCGAGGCGGAGCAAATCGAAGACCAAGACGAAGACGAAGAAGAAGAAGAGGGACTATGACTCCGGTTCGGGGTCTTCGAGTGGTGGCGGTGATTCTGAGCCGGAGAGGAGCCGGATTGAGCCGCAAGAGGTTTTGCGAGACTTGTTCAGTGAATTTCCTAATGTTGGGGACGATTTGAAGCAGCTTTTGCAAATGATTGATAGTGGGCAAGCAGTTGATATAAAGGGAATATCTGAAAGATCTTTGATTAAGCATCTAAAGAAGTTATTCCTTTCATTAGAACTCAAGGAAAGTGATGGAGTTTTTGTACTGCCTTCAAATGTTCGTCCCACCTTGGAGGTTGTTGGGGTCATGATTCCAATGTCTGTAGATCTTAAATCTTCATTAAATGAGATGCATTCAGAACAACGGGATGAGGAATACAAACAAGTGGTTGGTGATGATAATATGGCATCGCCATGTCCGGAGGATAATGTTGCTGGTCCTAACAGAAGGATAATTGGCCCTGCAATGCCATCGGCCGAGCTACTTGCTGCTGCAGCCAAACTGACAGAGGCACAGGCCGAGCTCAGGGAAGTGGAGTTGGAAGAGGATGCTGGAATGTTTATAGGGCCTCCACCACCTGCTGTTGTTGCTGAAGTTGAATCAGCTAATGAAGCTGAACGGTTTGAAGAGGTCACGAGGATCATAGATGCTGAGGAGAATAGTCCATATGATGTTCTAGGAGCAAACCAAAATATGGCTCCTGAGAACATAAAGAAAAGGTACTGGAAGATGTCTCTTTTGGTGCACCCTGATAAATGCTCACATCCTCAAGCTCAACAAGCATTTGTCAAGTTGAACAAAGCTTTTAAAGATTTACAAGATCCAGAAAAGCGGAAAGTGCTAGATGAGAAGATTAAACTCAAGGAGGAGCATGAGAAATTTAAGGTGGAGTTGCGAGCAATGCGGGAGGCTGCACAATGGAGAAAGTTACAAGGAATATCCATGGAGGGTGATGATGAGCTATTGGCAGACATGGATGTTAAAGCGGCACCACAAAGAGATGAGTGGATGACAACGCTTCCTCCTGAGAGGAGAGTAAGTCTTTCATTTTTTTGGTTTCATTTTTTATTTGACCTCTGGATTGGAGCTAACCTTATGATAAAATCTGTTGTCTGTCTATTACACATGTTCTTGTTGATATTTGTGGCTACTCTAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001664 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0050780
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3752 DnaJ

Protein Analysis

517

Amino Acids

58.91

Weight (kDa)

6.44

Isoelectric Point (pI)

69.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 353 - 413 3.6e-17 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 979
Acc36I ACCTGC 1 cut(s) 979
AccB1I GGYRCC 1 cut(s) 1381
AccB7I CCANNNNNTGG 2 cut(s) 97, 1087
AccBSI CCGCTC 1 cut(s) 243
AccIII TCCGGA 1 cut(s) 811
AciI CCGC 7 cut(s) 241, 355, 435, 470, 1225, 1297, 1379
AclWI GGATC 2 cut(s) 1040, 1208
AcoI YGGCCR 1 cut(s) 867
AcsI RAATTY 3 cut(s) 134, 500, 1271
AcuI CTGAAG 1 cut(s) 1005
AcyI GRCGYC 1 cut(s) 256
AfaI GTAC 3 cut(s) 82, 657, 1115
AfiI CCNNNNNNNGG 7 cut(s) 34, 97, 252, 417, 512, 918, 1087
AflIII ACRYGT 1 cut(s) 1518
AgsI TTSAA 7 cut(s) 122, 222, 526, 666, 992, 1019, 1189
Alw21I GWGCWC 3 cut(s) 131, 918, 1140
Alw26I GTCTC 3 cut(s) 219, 480, 1131
Alw44I GTGCAC 1 cut(s) 1136
AlwI GGATC 2 cut(s) 1040, 1208
Aor13HI TCCGGA 1 cut(s) 811
AoxI GGCC 6 cut(s) 37, 68, 850, 867, 909, 959
ApaLI GTGCAC 1 cut(s) 1136
ApeKI GCWGC 5 cut(s) 529, 881, 884, 887, 1304
ApoI RAATTY 3 cut(s) 134, 500, 1271
Asp700I GAANNNNTTC 1 cut(s) 614
AspS9I GGNCC 6 cut(s) 24, 37, 68, 830, 851, 959
AsuHPI GGTGA 4 cut(s) 195, 449, 797, 1355
AvaII GGWCC 2 cut(s) 24, 830
BaeGI GKGCMC 1 cut(s) 1140
BanI GGYRCC 1 cut(s) 1381
BanII GRGCYC 1 cut(s) 918
BauI CACGAG 1 cut(s) 1027
BbsI GAAGAC 3 cut(s) 374, 386, 414
Bbv12I GWGCWC 3 cut(s) 131, 918, 1140
BbvCI CCTCAGC 1 cut(s) 1044
BbvI GCAGC 5 cut(s) 541, 868, 871, 899, 1291
BccI CCATC 2 cut(s) 638, 871
BceAI ACGGC 3 cut(s) 269, 301, 347
BcgI CGANNNNNNTGC 4 cut(s) 846, 860, 880, 894
BcoDI GTCTC 3 cut(s) 219, 480, 1131
BfaI CTAG 4 cut(s) 29, 1070, 1235, 1547
BfmI CTRYAG 2 cut(s) 714, 885
BfuAI ACCTGC 1 cut(s) 979
BglII AGATCT 2 cut(s) 587, 718
BisI GCNGC 7 cut(s) 470, 530, 882, 885, 888, 1305, 1380
BlsI GCNGC 7 cut(s) 471, 531, 883, 886, 889, 1306, 1381
Bme18I GGWCC 2 cut(s) 24, 830
BmgT120I GGNCC 6 cut(s) 24, 37, 68, 830, 851, 959
BmiI GGNNCC 5 cut(s) 26, 289, 458, 1092, 1383
BmsI GCATC 5 cut(s) 610, 732, 809, 931, 1030
BpiI GAAGAC 3 cut(s) 374, 386, 414
BpmI CTGGAG 1 cut(s) 274
Bpu10I CCTNAGC 2 cut(s) 917, 1044
BpuEI CTTGAG 3 cut(s) 617, 1146, 1238
BsaHI GRCGYC 1 cut(s) 256
BsaJI CCNNGG 2 cut(s) 681, 1335
BsaWI WCCGGW 2 cut(s) 410, 811
Bsc4I CCNNNNNNNGG 7 cut(s) 34, 97, 252, 417, 512, 918, 1087
Bse1I ACTGG 1 cut(s) 1121
Bse3DI GCAATG 2 cut(s) 864, 1299
BseAI TCCGGA 1 cut(s) 811
BseDI CCNNGG 2 cut(s) 681, 1335
BseGI GGATG 5 cut(s) 766, 946, 1156, 1375, 1407
BseLI CCNNNNNNNGG 7 cut(s) 34, 97, 252, 417, 512, 918, 1087
BseMI GCAATG 2 cut(s) 864, 1299
BseMII CTCAG 5 cut(s) 435, 931, 1035, 1086, 1410
BseNI ACTGG 1 cut(s) 1121
BseRI GAGGAG 6 cut(s) 235, 334, 469, 1061, 1274, 1438
BseSI GKGCMC 1 cut(s) 1140
BseX3I CGGCCG 1 cut(s) 867
BseXI GCAGC 5 cut(s) 541, 868, 871, 899, 1291
BsgI GTGCAG 1 cut(s) 1290
Bsh1285I CGRYCG 2 cut(s) 241, 870
BshFI GGCC 6 cut(s) 39, 70, 852, 869, 911, 961
BshNI GGYRCC 1 cut(s) 1381
BsiEI CGRYCG 2 cut(s) 241, 870
BsiHKAI GWGCWC 3 cut(s) 131, 918, 1140
BsiSI CCGG 5 cut(s) 182, 411, 449, 460, 812
BslFI GGGAC 3 cut(s) 413, 530, 660
BslI CCNNNNNNNGG 7 cut(s) 34, 97, 252, 417, 512, 918, 1087
BsmAI GTCTC 3 cut(s) 219, 480, 1131
BsmBI CGTCTC 1 cut(s) 219
BsmFI GGGAC 3 cut(s) 413, 530, 660
BsmI GAATGC 1 cut(s) 745
BsnI GGCC 6 cut(s) 39, 70, 852, 869, 911, 961
Bsp1286I GDGCHC 3 cut(s) 131, 918, 1140
Bsp13I TCCGGA 1 cut(s) 811
Bsp143I GATC 4 cut(s) 587, 718, 1032, 1213
Bsp19I CCATGG 1 cut(s) 1335
BspACI CCGC 7 cut(s) 241, 355, 435, 470, 1225, 1297, 1379
BspANI GGCC 6 cut(s) 39, 70, 852, 869, 911, 961
BspCNI CTCAG 5 cut(s) 436, 930, 1036, 1087, 1411
BspEI TCCGGA 1 cut(s) 811
BspHI TCATGA 2 cut(s) 58, 699
BspLI GGNNCC 5 cut(s) 26, 289, 458, 1092, 1383
BspMAI CTGCAG 1 cut(s) 889
BspMI ACCTGC 1 cut(s) 979
BspPI GGATC 2 cut(s) 1040, 1208
BspT107I GGYRCC 1 cut(s) 1381
BsrBI CCGCTC 1 cut(s) 243
BsrDI GCAATG 2 cut(s) 864, 1299
BsrI ACTGG 1 cut(s) 1121
BssECI CCNNGG 2 cut(s) 681, 1335
BssMI GATC 4 cut(s) 587, 718, 1032, 1213
BssNI GRCGYC 1 cut(s) 256
BssSI CACGAG 1 cut(s) 1027
BssT1I CCWWGG 2 cut(s) 681, 1335
Bst2BI CACGAG 1 cut(s) 1027
Bst4CI ACNGT 4 cut(s) 126, 207, 269, 1014
Bst6I CTCTTC 4 cut(s) 289, 389, 930, 1014
BstACI GRCGYC 1 cut(s) 256
BstAPI GCANNNNNTGC 1 cut(s) 535
BstC8I GCNNGC 2 cut(s) 558, 1290
BstDEI CTNAG 5 cut(s) 444, 917, 1044, 1095, 1419
BstDSI CCRYGG 1 cut(s) 1335
BstF5I GGATG 5 cut(s) 766, 946, 1156, 1375, 1407
BstKTI GATC 4 cut(s) 590, 721, 1035, 1216
BstMAI GTCTC 3 cut(s) 219, 480, 1131
BstMBI GATC 4 cut(s) 587, 718, 1032, 1213
BstMCI CGRYCG 2 cut(s) 241, 870
BstMWI GCNNNNNNNGC 8 cut(s) 285, 535, 887, 980, 1004, 1172, 1231, 1294
BstNSI RCATGY 1 cut(s) 1522
BstSFI CTRYAG 2 cut(s) 714, 885
BstSLI GKGCMC 1 cut(s) 1140
BstV1I GCAGC 5 cut(s) 541, 868, 871, 899, 1291
BstV2I GAAGAC 3 cut(s) 374, 386, 414
BstX2I RGATCY 3 cut(s) 587, 718, 1213
BstYI RGATCY 3 cut(s) 587, 718, 1213
BstZI CGGCCG 1 cut(s) 867
BsuRI GGCC 6 cut(s) 39, 70, 852, 869, 911, 961
BtgI CCRYGG 1 cut(s) 1335
BtgZI GCGATG 1 cut(s) 786
BtsCI GGATG 5 cut(s) 766, 946, 1156, 1375, 1407
BtsIMutI CAGTG 2 cut(s) 131, 502
BveI ACCTGC 1 cut(s) 979
Cac8I GCNNGC 2 cut(s) 558, 1290
CciI TCATGA 2 cut(s) 58, 699
Cfr13I GGNCC 6 cut(s) 24, 37, 68, 830, 851, 959
CseI GACGC 1 cut(s) 245
Csp6I GTAC 3 cut(s) 81, 656, 1114
CviAII CATG 7 cut(s) 59, 700, 807, 1265, 1336, 1366, 1519
CviQI GTAC 3 cut(s) 81, 656, 1114
DdeI CTNAG 5 cut(s) 444, 917, 1044, 1095, 1419
DpnI GATC 4 cut(s) 589, 720, 1034, 1215
DpnII GATC 4 cut(s) 587, 718, 1032, 1213
DraI TTTAAA 1 cut(s) 1201
EaeI YGGCCR 1 cut(s) 867
EagI CGGCCG 1 cut(s) 867
Eam1104I CTCTTC 4 cut(s) 289, 389, 930, 1014
EarI CTCTTC 4 cut(s) 289, 389, 930, 1014
EciI GGCGGA 1 cut(s) 370
Ecl136II GAGCTC 1 cut(s) 916
EclXI CGGCCG 1 cut(s) 867
Eco130I CCWWGG 2 cut(s) 681, 1335
Eco24I GRGCYC 1 cut(s) 918
Eco47I GGWCC 2 cut(s) 24, 830
Eco52I CGGCCG 1 cut(s) 867
Eco53kI GAGCTC 1 cut(s) 916
Eco57I CTGAAG 1 cut(s) 1005
EcoICRI GAGCTC 1 cut(s) 916
EcoO109I RGGNCCY 1 cut(s) 959
EcoT14I CCWWGG 2 cut(s) 681, 1335
EcoT22I ATGCAT 1 cut(s) 747
EcoT38I GRGCYC 1 cut(s) 918
ErhI CCWWGG 2 cut(s) 681, 1335
Esp3I CGTCTC 1 cut(s) 219
FaeI CATG 7 cut(s) 62, 703, 810, 1268, 1339, 1369, 1522
FaqI GGGAC 3 cut(s) 413, 530, 660
FatI CATG 7 cut(s) 58, 699, 806, 1264, 1335, 1365, 1518
FauI CCCGC 1 cut(s) 1290
FauNDI CATATG 1 cut(s) 1060
Fnu4HI GCNGC 7 cut(s) 470, 530, 882, 885, 888, 1305, 1380
FokI GGATG 5 cut(s) 773, 953, 1143, 1382, 1414
FriOI GRGCYC 1 cut(s) 918
Fsp4HI GCNGC 7 cut(s) 470, 530, 882, 885, 888, 1305, 1380
FspBI CTAG 4 cut(s) 29, 1070, 1235, 1547
GluI GCNGC 7 cut(s) 470, 530, 882, 885, 888, 1305, 1380
GsuI CTGGAG 1 cut(s) 274
HaeIII GGCC 6 cut(s) 39, 70, 852, 869, 911, 961
HapII CCGG 5 cut(s) 182, 411, 449, 460, 812
HgaI GACGC 1 cut(s) 245
Hin1I GRCGYC 1 cut(s) 256
Hin1II CATG 7 cut(s) 62, 703, 810, 1268, 1339, 1369, 1522
HindIII AAGCTT 1 cut(s) 1194
HinfI GANTC 7 cut(s) 185, 200, 233, 407, 440, 703, 992
HpaII CCGG 5 cut(s) 182, 411, 449, 460, 812
HphI GGTGA 4 cut(s) 195, 449, 797, 1355
Hpy166II GTNNAC 3 cut(s) 11, 81, 1138
Hpy188I TCNGA 6 cut(s) 133, 229, 238, 445, 583, 751
Hpy188III TCNNGA 9 cut(s) 59, 291, 700, 812, 1027, 1094, 1217, 1418, 1471
Hpy8I GTNNAC 3 cut(s) 11, 81, 1138
Hpy99I CGWCG 7 cut(s) 197, 211, 214, 261, 264, 284, 287
HpyAV CCTTC 4 cut(s) 228, 306, 672, 834
HpyCH4III ACNGT 4 cut(s) 126, 207, 269, 1014
HpyCH4V TGCA 7 cut(s) 144, 538, 745, 857, 887, 1138, 1307
HpyF10VI GCNNNNNNNGC 8 cut(s) 285, 535, 887, 980, 1004, 1172, 1231, 1294
HpyF3I CTNAG 5 cut(s) 444, 917, 1044, 1095, 1419
Hsp92I GRCGYC 1 cut(s) 256
Hsp92II CATG 7 cut(s) 62, 703, 810, 1268, 1339, 1369, 1522
Kpn2I TCCGGA 1 cut(s) 811
Kzo9I GATC 4 cut(s) 587, 718, 1032, 1213
LmnI GCTCC 8 cut(s) 248, 293, 357, 456, 1073, 1096, 1261, 1478
Lsp1109I GCAGC 5 cut(s) 541, 868, 871, 899, 1291
LweI GCATC 5 cut(s) 610, 732, 809, 931, 1030
MaeI CTAG 4 cut(s) 29, 1070, 1235, 1547
MaeIII GTNAC 3 cut(s) 269, 1024, 1320
MalI GATC 4 cut(s) 589, 720, 1034, 1215
MbiI CCGCTC 1 cut(s) 243
MboI GATC 4 cut(s) 587, 718, 1032, 1213
MfeI CAATTG 1 cut(s) 176
MflI RGATCY 3 cut(s) 587, 718, 1213
MhlI GDGCHC 3 cut(s) 131, 918, 1140
MluCI AATT 5 cut(s) 134, 176, 500, 846, 1271
MlyI GAGTC 1 cut(s) 401
MmeI TCCRAC 2 cut(s) 261, 912
Mph1103I ATGCAT 1 cut(s) 747
MroI TCCGGA 1 cut(s) 811
MroXI GAANNNNTTC 1 cut(s) 614
MseI TTAA 7 cut(s) 597, 723, 734, 1200, 1248, 1275, 1374
MslI CAYNNNNRTG 1 cut(s) 805
MspI CCGG 5 cut(s) 182, 411, 449, 460, 812
MunI CAATTG 1 cut(s) 176
Mva1269I GAATGC 1 cut(s) 745
MwoI GCNNNNNNNGC 8 cut(s) 285, 535, 887, 980, 1004, 1172, 1231, 1294
NcoI CCATGG 1 cut(s) 1335
NdeI CATATG 1 cut(s) 1060
NdeII GATC 4 cut(s) 587, 718, 1032, 1213
NlaIII CATG 7 cut(s) 62, 703, 810, 1268, 1339, 1369, 1522
NlaIV GGNNCC 5 cut(s) 26, 289, 458, 1092, 1383
NmeAIII GCCGAG 2 cut(s) 895, 937
NmuCI GTSAC 2 cut(s) 269, 1024
NsiI ATGCAT 1 cut(s) 747
NspI RCATGY 1 cut(s) 1522
PagI TCATGA 2 cut(s) 58, 699
PaqCI CACCTGC 1 cut(s) 979
PciI ACATGT 1 cut(s) 1518
PcsI WCGNNNNNNNCGW 2 cut(s) 265, 422
PctI GAATGC 1 cut(s) 745
PdmI GAANNNNTTC 1 cut(s) 614
PfeI GAWTC 6 cut(s) 185, 200, 233, 440, 703, 992
PflMI CCANNNNNTGG 2 cut(s) 97, 1087
PkrI GCNGC 7 cut(s) 471, 531, 883, 886, 889, 1306, 1381
PleI GAGTC 1 cut(s) 401
PpsI GAGTC 1 cut(s) 401
PscI ACATGT 1 cut(s) 1518
Psp124BI GAGCTC 1 cut(s) 918
PspN4I GGNNCC 5 cut(s) 26, 289, 458, 1092, 1383
PspPI GGNCC 6 cut(s) 24, 37, 68, 830, 851, 959
PstI CTGCAG 1 cut(s) 889
PsuI RGATCY 3 cut(s) 587, 718, 1213
RsaI GTAC 3 cut(s) 82, 657, 1115
RsaNI GTAC 3 cut(s) 81, 656, 1114
RseI CAYNNNNRTG 1 cut(s) 805
SacI GAGCTC 1 cut(s) 918
SaqAI TTAA 7 cut(s) 597, 723, 734, 1200, 1248, 1275, 1374
SatI GCNGC 7 cut(s) 470, 530, 882, 885, 888, 1305, 1380
Sau3AI GATC 4 cut(s) 587, 718, 1032, 1213
Sau96I GGNCC 6 cut(s) 24, 37, 68, 830, 851, 959
SchI GAGTC 1 cut(s) 401
SduI GDGCHC 3 cut(s) 131, 918, 1140
SfaNI GCATC 5 cut(s) 610, 732, 809, 931, 1030
SfcI CTRYAG 2 cut(s) 714, 885
SinI GGWCC 2 cut(s) 24, 830
SmiMI CAYNNNNRTG 1 cut(s) 805
SmlI CTYRAG 3 cut(s) 632, 1161, 1253
SmoI CTYRAG 3 cut(s) 632, 1161, 1253
Sse9I AATT 5 cut(s) 134, 176, 500, 846, 1271
SsiI CCGC 7 cut(s) 241, 355, 435, 470, 1225, 1297, 1379
SspMI CTAG 4 cut(s) 29, 1070, 1235, 1547
SstI GAGCTC 1 cut(s) 918
StyI CCWWGG 2 cut(s) 681, 1335
TaaI ACNGT 4 cut(s) 126, 207, 269, 1014
TaqI TCGA 4 cut(s) 188, 248, 365, 425
TasI AATT 5 cut(s) 134, 176, 500, 846, 1271
TatI WGTACW 1 cut(s) 655
TauI GCSGC 2 cut(s) 472, 1382
TfiI GAWTC 6 cut(s) 185, 200, 233, 440, 703, 992
Tru1I TTAA 7 cut(s) 597, 723, 734, 1200, 1248, 1275, 1374
Tru9I TTAA 7 cut(s) 597, 723, 734, 1200, 1248, 1275, 1374
TscAI CASTG 2 cut(s) 131, 502
TseFI GTSAC 2 cut(s) 269, 1024
TseI GCWGC 5 cut(s) 529, 881, 884, 887, 1304
Tsp45I GTSAC 2 cut(s) 269, 1024
TspDTI ATGAA 7 cut(s) 17, 47, 612, 720, 1017, 1428, 1442
TspRI CASTG 2 cut(s) 131, 502
Van91I CCANNNNNTGG 2 cut(s) 97, 1087
VneI GTGCAC 1 cut(s) 1136
VpaK11BI GGWCC 2 cut(s) 24, 830
XapI RAATTY 3 cut(s) 134, 500, 1271
XceI RCATGY 1 cut(s) 1522
XmnI GAANNNNTTC 1 cut(s) 614
XspI CTAG 4 cut(s) 29, 1070, 1235, 1547
Zsp2I ATGCAT 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.