RchiOBHm_Chr5g0082081

Protein of unknown function (DUF3752)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001274
Physical Location & Seq
Forward (+)
87956612 .. 87957774
1163 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35630

Sequence Viewer

Length: 1095 bp
ATGAGATTGATGATGATTCTGGGTCTTCGAGCGGTGCTTTTGGAAGTGATTGATGGGGGCAAAGCGGTTGACATAAATGGACTAGCTCAGAAACATCTGATCAAGCCTCTCAGGACTCTATTCCTTTCCTTGAATCTCAAGGAACTGAATCACGGAGGTTTTGTACTGCGTCCAAATGTTCGTCTAACTTTGGAGGTTGTTGGTCCCATGATTGCGCCATCTTTAGATCATGTTGTTCCTCCTGAGAGAAGGCCAATTGGCCCGGAGAAGCCGTCGCCGGAGATACTTGATGCTGCAGCCAAGTTGAGAGATGCAGAGGCCGAGTTAAGGGAAGCTGAGTTGGAAGAAAAAGATGATGGGGTTTTTATTGGACCTGCACCACGTGTTGTGGCTGTTGAAGCTGAATCGGCTAGCCAAGATGATTGGTTTGAAGAGGTGACAAGGATCATGGAAGCAGAGGATGATAGCTCCTATGAGATACTAGGAGAAAGCCATTATGCGTCTGCTGATAACATTAAGAAAAGGTACTGGAAGTTGTCACTTTTGGTGCACCCTGATAAATGCTCACACCCTCAAGCCAACCAAGCATTTGTGAAGCTGTACAAGGCCTTTAAAGAACTACAGGATCCGAAGAAGCGGAGAGCGCTGGATGAGAAGATTGAGCTCAGGGAGAAGCAGGAGAGATTCGTTGTTAAGTTGAGGGCAATGCGGGAAGCAGCAATGTGGAGAAAGATGCAGGGGATTTCCATGGAGGGTGATGAGGAGCTACTAGCAGGAGATGTGAGTGGTGTTAAAGTGGCACGAAAAAGAGATGAGTGGATGACAGAGCTTCCACCGGAGAGGAAACATGGTATGAGTACAATGCAATCGACAATGTTTAGCAGGAGTGCTAAACAAGGGCGTGGGGATATTAGTGGATGGACTGATGCTCCTTCTGAAAGAGCTTGGAAAGCTAAGATGGATTACTTACAAGCCTATAATGAGTTTTCATCACTTGCTTCGAATGAGGAGGAGAGGAAGAGGAGTCTAGAAGCAGAGTTGATGGACAATTACAAGAAAGATAAACGATCAAAATCATTGCTACAAAAGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001664 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0050780
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3752 DnaJ

Protein Analysis

364

Amino Acids

41.51

Weight (kDa)

6.51

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 157 - 217 2.2e-16 DnaJ domain
DUF3752 PF12572 278 - 364 2e-11 Protein of unknown function (DUF3752)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 382
AccBSI CCGCTC 1 cut(s) 32
AciI CCGC 4 cut(s) 32, 65, 637, 709
AclWI GGATC 3 cut(s) 452, 620, 633
AcvI CACGTG 1 cut(s) 383
AdeI CACNNNGTG 1 cut(s) 383
AfaI GTAC 4 cut(s) 165, 527, 602, 859
AfeI AGCGCT 1 cut(s) 645
AfiI CCNNNNNNNGG 1 cut(s) 327
AflIII ACRYGT 1 cut(s) 382
AgsI TTSAA 3 cut(s) 133, 398, 431
Alw21I GWGCWC 2 cut(s) 552, 666
Alw44I GTGCAC 1 cut(s) 548
AlwI GGATC 3 cut(s) 452, 620, 633
Aor51HI AGCGCT 1 cut(s) 645
AoxI GGCC 4 cut(s) 251, 259, 318, 606
ApaLI GTGCAC 1 cut(s) 548
ApeKI GCWGC 3 cut(s) 293, 296, 716
AspLEI GCGC 2 cut(s) 217, 646
AspS9I GGNCC 3 cut(s) 203, 260, 371
AsuC2I CCSGG 1 cut(s) 263
AsuHPI GGTGA 2 cut(s) 448, 767
AsuII TTCGAA 1 cut(s) 1001
AsuNHI GCTAGC 1 cut(s) 410
AvaII GGWCC 2 cut(s) 203, 371
BaeGI GKGCMC 1 cut(s) 552
BamHI GGATCC 1 cut(s) 625
BanII GRGCYC 1 cut(s) 666
BbrPI CACGTG 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 17
Bbv12I GWGCWC 2 cut(s) 552, 666
BbvI GCAGC 3 cut(s) 280, 308, 728
BccI CCATC 6 cut(s) 47, 226, 350, 912, 952, 1036
BceAI ACGGC 1 cut(s) 256
BclI TGATCA 1 cut(s) 99
BcnI CCSGG 1 cut(s) 263
BfaI CTAG 5 cut(s) 83, 411, 482, 770, 1028
BfmI CTRYAG 2 cut(s) 294, 620
BfoI RGCGCY 1 cut(s) 647
BfuAI ACCTGC 1 cut(s) 382
BisI GCNGC 3 cut(s) 294, 297, 717
BlsI GCNGC 3 cut(s) 295, 298, 718
Bme1390I CCNGG 1 cut(s) 263
Bme18I GGWCC 2 cut(s) 203, 371
BmgT120I GGNCC 3 cut(s) 203, 260, 371
BmiI GGNNCC 2 cut(s) 205, 627
BmrFI CCNGG 1 cut(s) 263
BmsI GCATC 4 cut(s) 280, 301, 723, 916
BmtI GCTAGC 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 17
Bpu10I CCTNAGC 1 cut(s) 665
Bpu14I TTCGAA 1 cut(s) 1001
BpuEI CTTGAG 2 cut(s) 122, 558
BpuMI CCSGG 1 cut(s) 263
BsaAI YACGTR 1 cut(s) 383
BsaBI GATNNNNATC 1 cut(s) 1072
BsaJI CCNNGG 1 cut(s) 747
BsaWI WCCGGW 1 cut(s) 835
BsaXI ACNNNNNCTCC 6 cut(s) 147, 177, 257, 287, 913, 943
Bsc4I CCNNNNNNNGG 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 533
Bse3DI GCAATG 3 cut(s) 711, 726, 1076
Bse8I GATNNNNATC 1 cut(s) 1072
BseDI CCNNGG 1 cut(s) 747
BseGI GGATG 4 cut(s) 466, 655, 825, 923
BseJI GATNNNNATC 1 cut(s) 1072
BseLI CCNNNNNNNGG 1 cut(s) 327
BseMI GCAATG 3 cut(s) 711, 726, 1076
BseMII CTCAG 5 cut(s) 101, 124, 234, 327, 679
BseNI ACTGG 1 cut(s) 533
BseRI GAGGAG 4 cut(s) 776, 1022, 1025, 1036
BseSI GKGCMC 1 cut(s) 552
BseXI GCAGC 3 cut(s) 280, 308, 728
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 4 cut(s) 253, 261, 320, 608
BsiHKAI GWGCWC 2 cut(s) 552, 666
BsiSI CCGG 3 cut(s) 263, 278, 836
BslFI GGGAC 1 cut(s) 189
BslI CCNNNNNNNGG 1 cut(s) 327
BsmFI GGGAC 1 cut(s) 189
BsnI GGCC 4 cut(s) 253, 261, 320, 608
Bsp119I TTCGAA 1 cut(s) 1001
Bsp1286I GDGCHC 2 cut(s) 552, 666
Bsp1407I TGTACA 1 cut(s) 600
Bsp143I GATC 5 cut(s) 99, 226, 444, 625, 1067
Bsp19I CCATGG 1 cut(s) 747
BspACI CCGC 4 cut(s) 32, 65, 637, 709
BspANI GGCC 4 cut(s) 253, 261, 320, 608
BspCNI CTCAG 5 cut(s) 100, 123, 235, 328, 678
BspLI GGNNCC 2 cut(s) 205, 627
BspMAI CTGCAG 1 cut(s) 298
BspMI ACCTGC 1 cut(s) 382
BspOI GCTAGC 1 cut(s) 414
BspPI GGATC 3 cut(s) 452, 620, 633
BspT104I TTCGAA 1 cut(s) 1001
BsrBI CCGCTC 1 cut(s) 32
BsrDI GCAATG 3 cut(s) 711, 726, 1076
BsrGI TGTACA 1 cut(s) 600
BsrI ACTGG 1 cut(s) 533
BssECI CCNNGG 1 cut(s) 747
BssMI GATC 5 cut(s) 99, 226, 444, 625, 1067
BssT1I CCWWGG 1 cut(s) 747
Bst6I CTCTTC 2 cut(s) 426, 1013
BstAUI TGTACA 1 cut(s) 600
BstBAI YACGTR 1 cut(s) 383
BstBI TTCGAA 1 cut(s) 1001
BstC8I GCNNGC 1 cut(s) 412
BstDEI CTNAG 6 cut(s) 87, 110, 243, 336, 665, 954
BstDSI CCRYGG 1 cut(s) 747
BstF5I GGATG 4 cut(s) 466, 655, 825, 923
BstH2I RGCGCY 1 cut(s) 647
BstHHI GCGC 2 cut(s) 217, 646
BstKTI GATC 5 cut(s) 102, 229, 447, 628, 1070
BstMBI GATC 5 cut(s) 99, 226, 444, 625, 1067
BstMWI GCNNNNNNNGC 6 cut(s) 398, 407, 584, 643, 950, 1087
BstSCI CCNGG 1 cut(s) 261
BstSFI CTRYAG 2 cut(s) 294, 620
BstSLI GKGCMC 1 cut(s) 552
BstV1I GCAGC 3 cut(s) 280, 308, 728
BstV2I GAAGAC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BsuRI GGCC 4 cut(s) 253, 261, 320, 608
BtgI CCRYGG 1 cut(s) 747
BtsCI GGATG 4 cut(s) 466, 655, 825, 923
BveI ACCTGC 1 cut(s) 382
Cac8I GCNNGC 1 cut(s) 412
CfoI GCGC 2 cut(s) 217, 646
Cfr13I GGNCC 3 cut(s) 203, 260, 371
CseI GACGC 2 cut(s) 158, 489
Csp6I GTAC 4 cut(s) 164, 526, 601, 858
CviAII CATG 5 cut(s) 208, 230, 448, 748, 848
CviQI GTAC 4 cut(s) 164, 526, 601, 858
DdeI CTNAG 6 cut(s) 87, 110, 243, 336, 665, 954
DpnI GATC 5 cut(s) 101, 228, 446, 627, 1069
DpnII GATC 5 cut(s) 99, 226, 444, 625, 1067
DraI TTTAAA 1 cut(s) 613
DraIII CACNNNGTG 1 cut(s) 383
Eam1104I CTCTTC 2 cut(s) 426, 1013
EarI CTCTTC 2 cut(s) 426, 1013
Ecl136II GAGCTC 1 cut(s) 664
Eco130I CCWWGG 1 cut(s) 747
Eco147I AGGCCT 1 cut(s) 608
Eco24I GRGCYC 1 cut(s) 666
Eco47I GGWCC 2 cut(s) 203, 371
Eco47III AGCGCT 1 cut(s) 645
Eco53kI GAGCTC 1 cut(s) 664
Eco72I CACGTG 1 cut(s) 383
EcoICRI GAGCTC 1 cut(s) 664
EcoT14I CCWWGG 1 cut(s) 747
EcoT38I GRGCYC 1 cut(s) 666
ErhI CCWWGG 1 cut(s) 747
FaeI CATG 5 cut(s) 211, 233, 451, 751, 851
FaqI GGGAC 1 cut(s) 189
FatI CATG 5 cut(s) 207, 229, 447, 747, 847
FauI CCCGC 1 cut(s) 702
FbaI TGATCA 1 cut(s) 99
Fnu4HI GCNGC 3 cut(s) 294, 297, 717
FokI GGATG 4 cut(s) 473, 662, 832, 930
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 3 cut(s) 294, 297, 717
FspBI CTAG 5 cut(s) 83, 411, 482, 770, 1028
GlaI GCGC 2 cut(s) 216, 645
GluI GCNGC 3 cut(s) 294, 297, 717
HaeII RGCGCY 1 cut(s) 647
HaeIII GGCC 4 cut(s) 253, 261, 320, 608
HapII CCGG 3 cut(s) 263, 278, 836
HgaI GACGC 2 cut(s) 158, 489
HhaI GCGC 2 cut(s) 217, 646
Hin1II CATG 5 cut(s) 211, 233, 451, 751, 851
Hin6I GCGC 2 cut(s) 215, 644
HinP1I GCGC 2 cut(s) 215, 644
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 7 cut(s) 16, 115, 133, 148, 404, 684, 1024
HpaII CCGG 3 cut(s) 263, 278, 836
HphI GGTGA 2 cut(s) 448, 767
Hpy166II GTNNAC 2 cut(s) 70, 550
Hpy188I TCNGA 4 cut(s) 90, 99, 630, 937
Hpy188III TCNNGA 3 cut(s) 112, 242, 1028
Hpy8I GTNNAC 2 cut(s) 70, 550
Hpy99I CGWCG 1 cut(s) 277
HpyAV CCTTC 2 cut(s) 243, 942
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 6 cut(s) 296, 314, 377, 550, 736, 865
HpyF10VI GCNNNNNNNGC 6 cut(s) 398, 407, 584, 643, 950, 1087
HpyF3I CTNAG 6 cut(s) 87, 110, 243, 336, 665, 954
HpySE526I ACGT 1 cut(s) 382
Hsp92II CATG 5 cut(s) 211, 233, 451, 751, 851
HspAI GCGC 2 cut(s) 215, 644
Ksp22I TGATCA 1 cut(s) 99
Kzo9I GATC 5 cut(s) 99, 226, 444, 625, 1067
LmnI GCTCC 3 cut(s) 473, 763, 934
Lsp1109I GCAGC 3 cut(s) 280, 308, 728
LweI GCATC 4 cut(s) 280, 301, 723, 916
MaeI CTAG 5 cut(s) 83, 411, 482, 770, 1028
MaeII ACGT 1 cut(s) 382
MaeIII GTNAC 2 cut(s) 436, 537
MalI GATC 5 cut(s) 101, 228, 446, 627, 1069
MbiI CCGCTC 1 cut(s) 32
MboI GATC 5 cut(s) 99, 226, 444, 625, 1067
MboII GAAGA 6 cut(s) 17, 356, 443, 643, 667, 1030
MfeI CAATTG 1 cut(s) 255
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 2 cut(s) 552, 666
MluCI AATT 2 cut(s) 255, 1048
MlyI GAGTC 2 cut(s) 109, 1033
MmeI TCCRAC 1 cut(s) 321
MseI TTAA 5 cut(s) 326, 516, 612, 693, 792
MspI CCGG 3 cut(s) 263, 278, 836
MspR9I CCNGG 1 cut(s) 263
MunI CAATTG 1 cut(s) 255
MwoI GCNNNNNNNGC 6 cut(s) 398, 407, 584, 643, 950, 1087
NciI CCSGG 1 cut(s) 263
NcoI CCATGG 1 cut(s) 747
NdeII GATC 5 cut(s) 99, 226, 444, 625, 1067
NheI GCTAGC 1 cut(s) 410
NlaIII CATG 5 cut(s) 211, 233, 451, 751, 851
NlaIV GGNNCC 2 cut(s) 205, 627
NmeAIII GCCGAG 1 cut(s) 346
NmuCI GTSAC 2 cut(s) 436, 537
NspV TTCGAA 1 cut(s) 1001
PceI AGGCCT 1 cut(s) 608
PfeI GAWTC 5 cut(s) 16, 133, 148, 404, 684
PkrI GCNGC 3 cut(s) 295, 298, 718
PleI GAGTC 2 cut(s) 109, 1032
PmaCI CACGTG 1 cut(s) 383
PmlI CACGTG 1 cut(s) 383
PpsI GAGTC 2 cut(s) 109, 1032
Ppu21I YACGTR 1 cut(s) 383
Psp124BI GAGCTC 1 cut(s) 666
PspCI CACGTG 1 cut(s) 383
PspN4I GGNNCC 2 cut(s) 205, 627
PspPI GGNCC 3 cut(s) 203, 260, 371
PstI CTGCAG 1 cut(s) 298
PsuI RGATCY 1 cut(s) 625
RsaI GTAC 4 cut(s) 165, 527, 602, 859
RsaNI GTAC 4 cut(s) 164, 526, 601, 858
SacI GAGCTC 1 cut(s) 666
SaqAI TTAA 5 cut(s) 326, 516, 612, 693, 792
SatI GCNGC 3 cut(s) 294, 297, 717
Sau3AI GATC 5 cut(s) 99, 226, 444, 625, 1067
Sau96I GGNCC 3 cut(s) 203, 260, 371
SchI GAGTC 2 cut(s) 109, 1033
ScrFI CCNGG 1 cut(s) 263
SduI GDGCHC 2 cut(s) 552, 666
SfaNI GCATC 4 cut(s) 280, 301, 723, 916
SfcI CTRYAG 2 cut(s) 294, 620
SfuI TTCGAA 1 cut(s) 1001
SinI GGWCC 2 cut(s) 203, 371
SmlI CTYRAG 2 cut(s) 137, 573
SmoI CTYRAG 2 cut(s) 137, 573
Sse9I AATT 2 cut(s) 255, 1048
SseBI AGGCCT 1 cut(s) 608
SsiI CCGC 4 cut(s) 32, 65, 637, 709
SspMI CTAG 5 cut(s) 83, 411, 482, 770, 1028
SstI GAGCTC 1 cut(s) 666
StuI AGGCCT 1 cut(s) 608
StyD4I CCNGG 1 cut(s) 261
StyI CCWWGG 1 cut(s) 747
TaiI ACGT 1 cut(s) 385
TaqI TCGA 3 cut(s) 28, 869, 1001
TasI AATT 2 cut(s) 255, 1048
TatI WGTACW 3 cut(s) 163, 600, 857
TfiI GAWTC 5 cut(s) 16, 133, 148, 404, 684
Tru1I TTAA 5 cut(s) 326, 516, 612, 693, 792
Tru9I TTAA 5 cut(s) 326, 516, 612, 693, 792
TseFI GTSAC 2 cut(s) 436, 537
TseI GCWGC 3 cut(s) 293, 296, 716
Tsp45I GTSAC 2 cut(s) 436, 537
TspDTI ATGAA 1 cut(s) 978
TspGWI ACGGA 1 cut(s) 168
VneI GTGCAC 1 cut(s) 548
VpaK11BI GGWCC 2 cut(s) 203, 371
XbaI TCTAGA 1 cut(s) 1027
XspI CTAG 5 cut(s) 83, 411, 482, 770, 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.