Rmu_sc0001687.1_g000002

Protein of unknown function (DUF3752)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001687.1
OG0001274
Physical Location & Seq
Forward (+)
16867 .. 18480
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001687.1_g000002.1.cds

Sequence Viewer

Length: 1614 bp
atgggcagtaaaagtaagaggatgaaacatctaggctacacgagtaaccattctgtccgttcatcaatttcctctccttcaagctccgacgattctaaacgcttttccaagacgcgtcatctccaccgtcctgatgacacttccaagagaggcaaacagaggcaaagggacaaggccaagaaacagaggagcaaaagtgaggcaaggagaatgaaatcgaaagacaacgataagaagaagcagagagcttatgagattgatgatgattctgggtcttcaagcggtggtgatccgcaagcggttctgcgagacttgttgaaggaatttcctaatattgggtgcgatttgaagcagctattgcaagtgatcgatgggggcaaagcggttgacataaatggactagctcagaaacctctgatcaagcctctcaggactctgttcctttccttgaatctcaaggaactgaatcacggaggttttgtactgcctccaaatgttcgtccgactttggaggttgttggttccatgattacgtcttctttagatcatgttgttcctcctgagagaaggccaattggcccagagaagccgtcgcgggagatacttgatgctgcagccaaattgagggaggcagaggccgagttaagggaagctgagttggaagaaaaagatgatggggtttttattggacctgcaccacatgctgtggcggctgaagctgaatcagctaaccaggctgaccggtttgaagaggtgacaaggatcacggaagcagaggatgattgcccctatgagatactaggatcaaaccacaatgtgtcggctgataacatcaagaaaaggtactggaagttgtcacttttggtgcaccctgataaatgctcacaccctcaagccaaccaagcatttgtgaagctgaacaaggcctttaaagagctacaggatccggagaagcggagagcactggatgagaagattgaactcagggagaagcaggagagattcattgttgagttgagggcaatgcgggaagcagcaatgtggagaaagatgcaggggatttccatggagggtgatgaggagctactggcaggtgatgtgactggtgttaaagcggcacgaaaaagagatgagtggatgacagagcttccaccagagaggaaacatggtatgagtacaaggcagtcgacaatgtttagcaggagtgctaaacaaaggcgtggggataccagtggatggactgatgctccttctgaaagagcttggaaagctaagatggagtacttacaagcctataatgagtttgcatcacttgcttcgaatgaggaggataggaagaggagtttagtggcagagttgatggacaattacaagaaagataaacgatcgaaatcattgctgcaaaagcaccaagatgagattagagcaaagagaaaatactccaagcaacgggcagaggaggaagactgggtggggaaacatccgtggaagccttgggaccgtgagaaagatttggcaggtggtagacaacgtgtgaaattggattcaagaaacatgactcgaggtttgtcatctcggttttctggaggatcataccagaggaacttcctttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001664 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0050780
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3752 DnaJ

Protein Analysis

537

Amino Acids

61.37

Weight (kDa)

9.54

Isoelectric Point (pI)

54.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1082, 1508
Acc36I ACCTGC 3 cut(s) 700, 1082, 1508
AccB7I CCANNNNNTGG 1 cut(s) 1236
AccI GTMKAC 2 cut(s) 1187, 1525
AccII CGCG 2 cut(s) 115, 595
AccIII TCCGGA 1 cut(s) 946
AciI CCGC 9 cut(s) 282, 293, 299, 383, 595, 710, 955, 1027, 1115
AclWI GGATC 6 cut(s) 284, 770, 811, 938, 951, 1597
AcsI RAATTY 1 cut(s) 323
AcuI CTGAAG 1 cut(s) 735
AdeI CACNNNGTG 1 cut(s) 817
AfaI GTAC 4 cut(s) 483, 845, 1177, 1283
AfiI CCNNNNNNNGG 5 cut(s) 335, 624, 645, 1236, 1449
AflIII ACRYGT 2 cut(s) 113, 1531
AgeI ACCGGT 1 cut(s) 741
AgsI TTSAA 8 cut(s) 81, 279, 319, 349, 451, 749, 980, 1548
AjnI CCWGG 1 cut(s) 732
Alw21I GWGCWC 2 cut(s) 870, 964
Alw26I GTCTC 1 cut(s) 303
Alw44I GTGCAC 1 cut(s) 866
AlwI GGATC 6 cut(s) 284, 770, 811, 938, 951, 1597
Ama87I CYCGRG 1 cut(s) 1560
Aor13HI TCCGGA 1 cut(s) 946
AoxI GGCC 5 cut(s) 174, 569, 577, 636, 924
ApaLI GTGCAC 1 cut(s) 866
ApeKI GCWGC 5 cut(s) 352, 611, 614, 1034, 1399
ApoI RAATTY 1 cut(s) 323
ArsI GACNNNNNNTTYG 2 cut(s) 1549, 1581
AsiGI ACCGGT 1 cut(s) 741
AspS9I GGNCC 3 cut(s) 578, 689, 1498
AsuHPI GGTGA 4 cut(s) 299, 766, 1085, 1106
AsuII TTCGAA 1 cut(s) 1319
AvaI CYCGRG 1 cut(s) 1560
AvaII GGWCC 2 cut(s) 689, 1498
BaeGI GKGCMC 1 cut(s) 870
BamHI GGATCC 1 cut(s) 943
BauI CACGAG 1 cut(s) 40
BbsI GAAGAC 3 cut(s) 267, 528, 1470
Bbv12I GWGCWC 2 cut(s) 870, 964
BbvI GCAGC 5 cut(s) 364, 598, 626, 1046, 1386
BccI CCATC 5 cut(s) 365, 668, 1230, 1270, 1354
BceAI ACGGC 1 cut(s) 574
BcgI CGANNNNNNTGC 2 cut(s) 1378, 1412
BciT130I CCWGG 1 cut(s) 734
BciVI GTATCC 1 cut(s) 1219
BclI TGATCA 1 cut(s) 417
BcoDI GTCTC 1 cut(s) 303
BfaI CTAG 3 cut(s) 32, 401, 800
BfmI CTRYAG 2 cut(s) 612, 938
BfuAI ACCTGC 3 cut(s) 700, 1082, 1508
BfuI GTATCC 1 cut(s) 1219
BisI GCNGC 7 cut(s) 353, 612, 615, 711, 1035, 1116, 1400
BlsI GCNGC 7 cut(s) 354, 613, 616, 712, 1036, 1117, 1401
BmcAI AGTACT 1 cut(s) 1283
Bme1390I CCNGG 1 cut(s) 734
Bme18I GGWCC 2 cut(s) 689, 1498
BmeT110I CYCGRG 1 cut(s) 1560
BmgT120I GGNCC 3 cut(s) 578, 689, 1498
BmiI GGNNCC 3 cut(s) 523, 945, 1499
BmrFI CCNGG 1 cut(s) 734
BmrI ACTGGG 1 cut(s) 1477
BmsI GCATC 4 cut(s) 598, 1041, 1234, 1316
BmuI ACTGGG 1 cut(s) 1477
BpiI GAAGAC 3 cut(s) 267, 528, 1470
BpmI CTGGAG 1 cut(s) 1605
Bpu14I TTCGAA 1 cut(s) 1319
BpuEI CTTGAG 2 cut(s) 440, 876
Bsa29I ATCGAT 1 cut(s) 369
BsaBI GATNNNNATC 1 cut(s) 1390
BsaJI CCNNGG 3 cut(s) 1065, 1484, 1493
BsaWI WCCGGW 2 cut(s) 741, 946
BsaXI ACNNNNNCTCC 6 cut(s) 181, 211, 465, 495, 1231, 1261
Bsc4I CCNNNNNNNGG 5 cut(s) 335, 624, 645, 1236, 1449
Bse118I RCCGGY 1 cut(s) 741
Bse1I ACTGG 6 cut(s) 851, 969, 1092, 1108, 1230, 1472
Bse3DI GCAATG 3 cut(s) 1029, 1044, 1394
Bse8I GATNNNNATC 1 cut(s) 1390
BseAI TCCGGA 1 cut(s) 946
BseBI CCWGG 1 cut(s) 734
BseCI ATCGAT 1 cut(s) 369
BseDI CCNNGG 3 cut(s) 1065, 1484, 1493
BseGI GGATG 6 cut(s) 27, 784, 973, 1143, 1241, 1480
BseJI GATNNNNATC 1 cut(s) 1390
BseLI CCNNNNNNNGG 5 cut(s) 335, 624, 645, 1236, 1449
BseMI GCAATG 3 cut(s) 1029, 1044, 1394
BseMII CTCAG 5 cut(s) 419, 442, 552, 645, 997
BseNI ACTGG 6 cut(s) 851, 969, 1092, 1108, 1230, 1472
BseRI GAGGAG 5 cut(s) 202, 1094, 1340, 1354, 1472
BseSI GKGCMC 1 cut(s) 870
BseXI GCAGC 5 cut(s) 364, 598, 626, 1046, 1386
BsgI GTGCAG 1 cut(s) 678
Bsh1236I CGCG 2 cut(s) 115, 595
Bsh1285I CGRYCG 1 cut(s) 1388
BshFI GGCC 5 cut(s) 176, 571, 579, 638, 926
BshTI ACCGGT 1 cut(s) 741
BshVI ATCGAT 1 cut(s) 369
BsiEI CGRYCG 1 cut(s) 1388
BsiHKAI GWGCWC 2 cut(s) 870, 964
BsiHKCI CYCGRG 1 cut(s) 1560
BsiSI CCGG 2 cut(s) 742, 947
BslFI GGGAC 2 cut(s) 182, 1511
BslI CCNNNNNNNGG 5 cut(s) 335, 624, 645, 1236, 1449
BsmAI GTCTC 1 cut(s) 303
BsmFI GGGAC 2 cut(s) 182, 1511
BsnI GGCC 5 cut(s) 176, 571, 579, 638, 926
BsoBI CYCGRG 1 cut(s) 1560
Bsp119I TTCGAA 1 cut(s) 1319
Bsp1286I GDGCHC 2 cut(s) 870, 964
Bsp13I TCCGGA 1 cut(s) 946
Bsp143I GATC 9 cut(s) 289, 366, 417, 544, 762, 803, 943, 1385, 1589
Bsp19I CCATGG 1 cut(s) 1065
BspACI CCGC 9 cut(s) 282, 293, 299, 383, 595, 710, 955, 1027, 1115
BspANI GGCC 5 cut(s) 176, 571, 579, 638, 926
BspCNI CTCAG 5 cut(s) 418, 441, 553, 646, 996
BspDI ATCGAT 1 cut(s) 369
BspEI TCCGGA 1 cut(s) 946
BspFNI CGCG 2 cut(s) 115, 595
BspLI GGNNCC 3 cut(s) 523, 945, 1499
BspMAI CTGCAG 1 cut(s) 616
BspMI ACCTGC 3 cut(s) 700, 1082, 1508
BspPI GGATC 6 cut(s) 284, 770, 811, 938, 951, 1597
BspT104I TTCGAA 1 cut(s) 1319
BsrDI GCAATG 3 cut(s) 1029, 1044, 1394
BsrFI RCCGGY 1 cut(s) 741
BsrI ACTGG 6 cut(s) 851, 969, 1092, 1108, 1230, 1472
BssAI RCCGGY 1 cut(s) 741
BssECI CCNNGG 3 cut(s) 1065, 1484, 1493
BssMI GATC 9 cut(s) 289, 366, 417, 544, 762, 803, 943, 1385, 1589
BssSI CACGAG 1 cut(s) 40
BssT1I CCWWGG 2 cut(s) 1065, 1493
Bst2BI CACGAG 1 cut(s) 40
Bst2UI CCWGG 1 cut(s) 734
Bst4CI ACNGT 2 cut(s) 128, 1502
Bst6I CTCTTC 2 cut(s) 744, 1331
BstAPI GCANNNNNTGC 3 cut(s) 358, 701, 1313
BstBI TTCGAA 1 cut(s) 1319
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 6 cut(s) 405, 428, 561, 654, 983, 1272
BstDSI CCRYGG 2 cut(s) 1065, 1484
BstF5I GGATG 6 cut(s) 27, 784, 973, 1143, 1241, 1480
BstFNI CGCG 2 cut(s) 115, 595
BstKTI GATC 9 cut(s) 292, 369, 420, 547, 765, 806, 946, 1388, 1592
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 9 cut(s) 289, 366, 417, 544, 762, 803, 943, 1385, 1589
BstMCI CGRYCG 1 cut(s) 1388
BstNI CCWGG 1 cut(s) 734
BstNSI RCATGY 1 cut(s) 704
BstSCI CCNGG 1 cut(s) 732
BstSFI CTRYAG 2 cut(s) 612, 938
BstSLI GKGCMC 1 cut(s) 870
BstUI CGCG 2 cut(s) 115, 595
BstV1I GCAGC 5 cut(s) 364, 598, 626, 1046, 1386
BstV2I GAAGAC 3 cut(s) 267, 528, 1470
BstX2I RGATCY 1 cut(s) 943
BstYI RGATCY 1 cut(s) 943
Bsu15I ATCGAT 1 cut(s) 369
BsuI GTATCC 1 cut(s) 1219
BsuRI GGCC 5 cut(s) 176, 571, 579, 638, 926
BsuTUI ATCGAT 1 cut(s) 369
BtgI CCRYGG 2 cut(s) 1065, 1484
BtsCI GGATG 6 cut(s) 27, 784, 973, 1143, 1241, 1480
BtsIMutI CAGTG 2 cut(s) 962, 1237
BveI ACCTGC 3 cut(s) 700, 1082, 1508
Cac8I GCNNGC 1 cut(s) 297
Cfr10I RCCGGY 1 cut(s) 741
Cfr13I GGNCC 3 cut(s) 578, 689, 1498
ClaI ATCGAT 1 cut(s) 369
CseI GACGC 2 cut(s) 104, 121
Csp6I GTAC 4 cut(s) 482, 844, 1176, 1282
CspAI ACCGGT 1 cut(s) 741
CviAII CATG 6 cut(s) 526, 548, 701, 1066, 1166, 1555
CviQI GTAC 4 cut(s) 482, 844, 1176, 1282
DdeI CTNAG 6 cut(s) 405, 428, 561, 654, 983, 1272
DpnI GATC 9 cut(s) 291, 368, 419, 546, 764, 805, 945, 1387, 1591
DpnII GATC 9 cut(s) 289, 366, 417, 544, 762, 803, 943, 1385, 1589
DraI TTTAAA 1 cut(s) 931
DraIII CACNNNGTG 1 cut(s) 817
Eam1104I CTCTTC 2 cut(s) 744, 1331
EarI CTCTTC 2 cut(s) 744, 1331
Eco130I CCWWGG 2 cut(s) 1065, 1493
Eco147I AGGCCT 1 cut(s) 926
Eco47I GGWCC 2 cut(s) 689, 1498
Eco57I CTGAAG 1 cut(s) 735
Eco88I CYCGRG 1 cut(s) 1560
EcoRII CCWGG 1 cut(s) 732
EcoT14I CCWWGG 2 cut(s) 1065, 1493
ErhI CCWWGG 2 cut(s) 1065, 1493
FaeI CATG 6 cut(s) 529, 551, 704, 1069, 1169, 1558
FaqI GGGAC 2 cut(s) 182, 1511
FatI CATG 6 cut(s) 525, 547, 700, 1065, 1165, 1554
FauI CCCGC 2 cut(s) 588, 1020
FbaI TGATCA 1 cut(s) 417
FblI GTMKAC 2 cut(s) 1187, 1525
Fnu4HI GCNGC 7 cut(s) 353, 612, 615, 711, 1035, 1116, 1400
FokI GGATG 6 cut(s) 34, 791, 980, 1150, 1248, 1467
Fsp4HI GCNGC 7 cut(s) 353, 612, 615, 711, 1035, 1116, 1400
FspBI CTAG 3 cut(s) 32, 401, 800
GluI GCNGC 7 cut(s) 353, 612, 615, 711, 1035, 1116, 1400
GsuI CTGGAG 1 cut(s) 1605
HaeIII GGCC 5 cut(s) 176, 571, 579, 638, 926
HapII CCGG 2 cut(s) 742, 947
HgaI GACGC 2 cut(s) 104, 121
Hin1II CATG 6 cut(s) 529, 551, 704, 1069, 1169, 1558
HincII GTYRAC 2 cut(s) 388, 1188
HindII GTYRAC 2 cut(s) 388, 1188
HinfI GANTC 9 cut(s) 92, 266, 433, 451, 466, 722, 1002, 1544, 1558
HpaII CCGG 2 cut(s) 742, 947
HphI GGTGA 4 cut(s) 299, 766, 1085, 1106
Hpy166II GTNNAC 4 cut(s) 388, 868, 1188, 1526
Hpy188I TCNGA 5 cut(s) 88, 408, 417, 504, 1255
Hpy188III TCNNGA 7 cut(s) 131, 430, 560, 835, 947, 1548, 1584
Hpy8I GTNNAC 4 cut(s) 388, 868, 1188, 1526
Hpy99I CGWCG 2 cut(s) 92, 595
HpyAV CCTTC 4 cut(s) 87, 313, 561, 1260
HpyCH4III ACNGT 2 cut(s) 128, 1502
HpyCH4IV ACGT 2 cut(s) 533, 1531
HpyCH4V TGCA 7 cut(s) 361, 614, 695, 868, 1054, 1307, 1402
HpyF3I CTNAG 6 cut(s) 405, 428, 561, 654, 983, 1272
HpySE526I ACGT 2 cut(s) 533, 1531
Hsp92II CATG 6 cut(s) 529, 551, 704, 1069, 1169, 1558
Kpn2I TCCGGA 1 cut(s) 946
Ksp22I TGATCA 1 cut(s) 417
Kzo9I GATC 9 cut(s) 289, 366, 417, 544, 762, 803, 943, 1385, 1589
LmnI GCTCC 4 cut(s) 89, 189, 1081, 1252
Lsp1109I GCAGC 5 cut(s) 364, 598, 626, 1046, 1386
LweI GCATC 4 cut(s) 598, 1041, 1234, 1316
MaeI CTAG 3 cut(s) 32, 401, 800
MaeII ACGT 2 cut(s) 533, 1531
MaeIII GTNAC 4 cut(s) 44, 754, 855, 1099
MalI GATC 9 cut(s) 291, 368, 419, 546, 764, 805, 945, 1387, 1591
MboI GATC 9 cut(s) 289, 366, 417, 544, 762, 803, 943, 1385, 1589
MboII GAAGA 8 cut(s) 247, 267, 528, 674, 761, 985, 1348, 1475
MfeI CAATTG 1 cut(s) 573
MflI RGATCY 1 cut(s) 943
MhlI GDGCHC 2 cut(s) 870, 964
MluCI AATT 6 cut(s) 66, 323, 573, 620, 1366, 1538
MluI ACGCGT 1 cut(s) 113
MlyI GAGTC 2 cut(s) 427, 1552
MmeI TCCRAC 3 cut(s) 111, 527, 639
MroI TCCGGA 1 cut(s) 946
MseI TTAA 3 cut(s) 644, 930, 1110
MslI CAYNNNNRTG 1 cut(s) 1413
MspI CCGG 2 cut(s) 742, 947
MspR9I CCNGG 1 cut(s) 734
MunI CAATTG 1 cut(s) 573
MvaI CCWGG 1 cut(s) 734
MvnI CGCG 2 cut(s) 115, 595
NcoI CCATGG 1 cut(s) 1065
NdeII GATC 9 cut(s) 289, 366, 417, 544, 762, 803, 943, 1385, 1589
NlaIII CATG 6 cut(s) 529, 551, 704, 1069, 1169, 1558
NlaIV GGNNCC 3 cut(s) 523, 945, 1499
NmeAIII GCCGAG 1 cut(s) 664
NmuCI GTSAC 3 cut(s) 754, 855, 1099
NspI RCATGY 1 cut(s) 704
NspV TTCGAA 1 cut(s) 1319
PaeR7I CTCGAG 1 cut(s) 1560
PaqCI CACCTGC 2 cut(s) 1082, 1508
PceI AGGCCT 1 cut(s) 926
PfeI GAWTC 7 cut(s) 92, 266, 451, 466, 722, 1002, 1544
PflMI CCANNNNNTGG 1 cut(s) 1236
PinAI ACCGGT 1 cut(s) 741
PkrI GCNGC 7 cut(s) 354, 613, 616, 712, 1036, 1117, 1401
Ple19I CGATCG 1 cut(s) 1388
PleI GAGTC 2 cut(s) 427, 1552
PpsI GAGTC 2 cut(s) 427, 1552
Psp6I CCWGG 1 cut(s) 732
PspGI CCWGG 1 cut(s) 732
PspN4I GGNNCC 3 cut(s) 523, 945, 1499
PspPI GGNCC 3 cut(s) 578, 689, 1498
PspXI VCTCGAGB 1 cut(s) 1560
PstI CTGCAG 1 cut(s) 616
PsuI RGATCY 1 cut(s) 943
PvuI CGATCG 1 cut(s) 1388
RsaI GTAC 4 cut(s) 483, 845, 1177, 1283
RsaNI GTAC 4 cut(s) 482, 844, 1176, 1282
RseI CAYNNNNRTG 1 cut(s) 1413
SalI GTCGAC 1 cut(s) 1186
SaqAI TTAA 3 cut(s) 644, 930, 1110
SatI GCNGC 7 cut(s) 353, 612, 615, 711, 1035, 1116, 1400
Sau3AI GATC 9 cut(s) 289, 366, 417, 544, 762, 803, 943, 1385, 1589
Sau96I GGNCC 3 cut(s) 578, 689, 1498
ScaI AGTACT 1 cut(s) 1283
SchI GAGTC 2 cut(s) 427, 1552
ScrFI CCNGG 1 cut(s) 734
SduI GDGCHC 2 cut(s) 870, 964
SfaNI GCATC 4 cut(s) 598, 1041, 1234, 1316
SfcI CTRYAG 2 cut(s) 612, 938
Sfr274I CTCGAG 1 cut(s) 1560
SfuI TTCGAA 1 cut(s) 1319
SinI GGWCC 2 cut(s) 689, 1498
SlaI CTCGAG 1 cut(s) 1560
SmiMI CAYNNNNRTG 1 cut(s) 1413
SmlI CTYRAG 3 cut(s) 455, 891, 1560
SmoI CTYRAG 3 cut(s) 455, 891, 1560
Sse9I AATT 6 cut(s) 66, 323, 573, 620, 1366, 1538
SseBI AGGCCT 1 cut(s) 926
SsiI CCGC 9 cut(s) 282, 293, 299, 383, 595, 710, 955, 1027, 1115
SspI AATATT 1 cut(s) 334
SspMI CTAG 3 cut(s) 32, 401, 800
StuI AGGCCT 1 cut(s) 926
StyD4I CCNGG 1 cut(s) 732
StyI CCWWGG 2 cut(s) 1065, 1493
TaaI ACNGT 2 cut(s) 128, 1502
TaiI ACGT 2 cut(s) 536, 1534
TaqI TCGA 6 cut(s) 218, 369, 1187, 1319, 1388, 1561
TasI AATT 6 cut(s) 66, 323, 573, 620, 1366, 1538
TatI WGTACW 3 cut(s) 481, 1175, 1281
TauI GCSGC 2 cut(s) 713, 1118
TfiI GAWTC 7 cut(s) 92, 266, 451, 466, 722, 1002, 1544
Tru1I TTAA 3 cut(s) 644, 930, 1110
Tru9I TTAA 3 cut(s) 644, 930, 1110
TscAI CASTG 2 cut(s) 969, 1237
TseFI GTSAC 3 cut(s) 754, 855, 1099
TseI GCWGC 5 cut(s) 352, 611, 614, 1034, 1399
Tsp45I GTSAC 3 cut(s) 754, 855, 1099
TspDTI ATGAA 4 cut(s) 38, 51, 227, 994
TspGWI ACGGA 4 cut(s) 47, 486, 782, 1473
TspRI CASTG 2 cut(s) 969, 1237
Van91I CCANNNNNTGG 1 cut(s) 1236
VneI GTGCAC 1 cut(s) 866
VpaK11BI GGWCC 2 cut(s) 689, 1498
XapI RAATTY 1 cut(s) 323
XceI RCATGY 1 cut(s) 704
XhoI CTCGAG 1 cut(s) 1560
XmiI GTMKAC 2 cut(s) 1187, 1525
XspI CTAG 3 cut(s) 32, 401, 800
ZrmI AGTACT 1 cut(s) 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.